Saccharomyces cerevisiae S288C chromosome I, complete sequence.
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources
The nucleotide sequence of chromosome I from Saccharomyces cerevisiae
Submitted (13-JAN-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
Submitted (11-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA
REVIEWED REFSEQ: This record has been curated by SGD. The reference\nsequence is identical to BK006935.\n\nOn Apr 26, 2011 this sequence version replaced NC_001133.8.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: SGD\nAnnotation Status :: Full Annotation\nAnnotation Version :: R64-3-1\nURL :: http://www.yeastgenome.org/\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
TEL01L; Telomeric region on the left arm of Chromosome I; composed of an X element core sequence, X element combinatorial repeats, and a short terminal stretch of telomeric repeats
ARS102; Autonomously Replicating Sequence
EXISTENCE:mutant phenotype:GO:0030437 ascospore formation [PMID:12586695]
hypothetical protein; member of the seripauperin multigene family encoded mainly in subtelomeric regions
hypothetical protein; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
EXISTENCE:direct assay:GO:0071944 cell periphery [PMID:26928762]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:8893857]
Putative permease; member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide
ARS103; Autonomously Replicating Sequence; replication origin of very weak function
hypothetical protein; shows sequence similarity to FLO1 and other flocculins
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762]
hypothetical protein; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene
hypothetical protein; may interact with ribosomes, based on co-purification experiments
hypothetical protein; identified by expression profiling and mass spectrometry
EXISTENCE:mutant phenotype:GO:0000128 flocculation [PMID:19420680|PMID:19087208]
EXISTENCE:mutant phenotype:GO:0031589 cell-substrate adhesion [PMID:19087208]
Lectin-like protein with similarity to Flo1p; thought to be expressed and involved in flocculation
ARS104; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004354 glutamate dehydrogenase (NADP+) activity [PMID:11562373]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:4126]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14576278|PMID:16823961]
EXISTENCE:mutant phenotype:GO:0004354 glutamate dehydrogenase (NADP+) activity [PMID:9287019]
EXISTENCE:mutant phenotype:GO:0006537 glutamate biosynthetic process [PMID:9287019]
NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095]
Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3
EXISTENCE:direct assay:GO:0000721 (R,R)-butanediol dehydrogenase activity [PMID:10938079]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11914276|PMID:14562095]
EXISTENCE:mutant phenotype:GO:0006066 alcohol metabolic process [PMID:10938079]
EXISTENCE:mutant phenotype:GO:0034079 butanediol biosynthetic process [PMID:19966022]
NAD-dependent (R,R)-butanediol dehydrogenase; catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11583615|PMID:14562095]
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:11583615]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:20348449]
EXISTENCE:genetic interaction:GO:0000055 ribosomal large subunit export from nucleus [PMID:11583615|PMID:20348449]
Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:7814381]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:16002399]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:15173200]
EXISTENCE:mutant phenotype:GO:0030433 ubiquitin-dependent ERAD pathway [PMID:7814381]
EXISTENCE:mutant phenotype:GO:0051082 unfolded protein binding [PMID:15173200]
EXISTENCE:physical interaction:GO:0005515 protein binding [PMID:16002399]
Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16030250]
EXISTENCE:genetic interaction:GO:0001403 invasive growth in response to glucose limitation [PMID:12150916]
EXISTENCE:genetic interaction:GO:0004862 cAMP-dependent protein kinase inhibitor activity [PMID:16278446|PMID:16924114]
EXISTENCE:genetic interaction:GO:0007124 pseudohyphal growth [PMID:12150916]
EXISTENCE:genetic interaction:GO:0010255 glucose mediated signaling pathway [PMID:12150916]
EXISTENCE:genetic interaction:GO:0046580 negative regulation of Ras protein signal transduction [PMID:16793550]
EXISTENCE:mutant phenotype:GO:0001403 invasive growth in response to glucose limitation [PMID:12150916]
EXISTENCE:mutant phenotype:GO:0007124 pseudohyphal growth [PMID:12150916]
EXISTENCE:mutant phenotype:GO:0010255 glucose mediated signaling pathway [PMID:12150916]
EXISTENCE:physical interaction:GO:0032794 GTPase activating protein binding [PMID:16793550]
Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication
ARS105; Autonomously Replicating Sequence
ubiquitin-protein transferase activating protein PEX22
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:24390141]
EXISTENCE:direct assay:GO:0031398 positive regulation of protein ubiquitination [PMID:25162638]
EXISTENCE:direct assay:GO:0097027 ubiquitin-protein transferase activator activity [PMID:22085930]
EXISTENCE:direct assay:GO:1902499 positive regulation of protein autoubiquitination [PMID:25162638]
EXISTENCE:mutant phenotype:GO:0016562 protein import into peroxisome matrix, receptor recycling [PMID:17452527]
Putative peroxisomal membrane protein; required for import of peroxisomal proteins; functionally complements a Pichia pastoris pex22 mutation
EXISTENCE:direct assay:GO:0003987 acetate-CoA ligase activity [PMID:8910545]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:9711835]
EXISTENCE:direct assay:GO:0006085 acetyl-CoA biosynthetic process [PMID:8910545]
EXISTENCE:direct assay:GO:0016880 acid-ammonia (or amide) ligase activity [PMID:18305111]
EXISTENCE:mutant phenotype:GO:0019654 acetate fermentation [PMID:1363452]
Acetyl-coA synthetase isoform; along with Acs2p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions
flavin adenine dinucleotide transporter FLC2
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:16717099]
EXISTENCE:genetic interaction:GO:0030148 sphingolipid biosynthetic process [PMID:27462707]
EXISTENCE:mutant phenotype:GO:0006457 protein folding [PMID:16717099]
EXISTENCE:mutant phenotype:GO:0009272 fungal-type cell wall biogenesis [PMID:16717099]
EXISTENCE:mutant phenotype:GO:0015230 FAD transmembrane transporter activity [PMID:16717099]
EXISTENCE:mutant phenotype:GO:0015883 FAD transport [PMID:16717099]
EXISTENCE:mutant phenotype:GO:0055074 calcium ion homeostasis [PMID:25573187]
EXISTENCE:mutant phenotype:GO:0055085 transmembrane transport [PMID:16717099]
EXISTENCE:mutant phenotype:GO:0071476 cellular hypotonic response [PMID:25573187]
Putative calcium channel involved in calcium release under hypotonic stress; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; targeted to vacuole via AP-3 pathway; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
oleate-activated transcription factor OAF1
EXISTENCE:direct assay:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:9288897]
EXISTENCE:direct assay:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:10428786]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:19111667|PMID:19158363]
EXISTENCE:direct assay:GO:0089716 Pip2-Oaf1 complex [PMID:9288897]
EXISTENCE:genetic interaction:GO:0061429 positive regulation of transcription from RNA polymerase II promoter by oleic acid [PMID:9288897]
EXISTENCE:mutant phenotype:GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding [PMID:9288897]
EXISTENCE:mutant phenotype:GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific [PMID:8972187|PMID:10428786]
EXISTENCE:mutant phenotype:GO:0032000 positive regulation of fatty acid beta-oxidation [PMID:8972187]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:8972187]
EXISTENCE:mutant phenotype:GO:0061429 positive regulation of transcription from RNA polymerase II promoter by oleic acid [PMID:9288897]
EXISTENCE:physical interaction:GO:0089716 Pip2-Oaf1 complex [PMID:8972187]
Oleate-activated transcription factor; subunit of a heterodimeric complex with Pip2p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; regulates chromatin silencing at telomeres; involved in diauxic shift; OAF1 has a paralog, PIP2, that arose from the whole genome duplication
Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor
ERMES complex Ca(2+)-binding regulatory GTPase GEM1
EXISTENCE:direct assay:GO:0005509 calcium ion binding [PMID:21036903]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:15479738|PMID:16407407]
EXISTENCE:genetic interaction:GO:0000001 mitochondrion inheritance [PMID:18245340]
EXISTENCE:genetic interaction:GO:0055091 phospholipid homeostasis [PMID:21825164]
EXISTENCE:mutant phenotype:GO:0000001 mitochondrion inheritance [PMID:18245340]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:15479738]
EXISTENCE:mutant phenotype:GO:0010821 regulation of mitochondrion organization [PMID:21825164]
EXISTENCE:mutant phenotype:GO:0015886 heme transport [PMID:32265272]
EXISTENCE:mutant phenotype:GO:1990456 mitochondrion-endoplasmic reticulum membrane tethering [PMID:21825164|PMID:23682313]
EXISTENCE:physical interaction:GO:0032865 ERMES complex [PMID:21825164|PMID:21945531]
Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:9382872]
EXISTENCE:direct assay:GO:0005824 outer plaque of spindle pole body [PMID:9670012]
EXISTENCE:direct assay:GO:0005876 spindle microtubule [PMID:9382872]
EXISTENCE:direct assay:GO:0044877 protein-containing complex binding [PMID:12970190|PMID:9670012]
EXISTENCE:direct assay:GO:0051010 microtubule plus-end binding [PMID:12970190]
EXISTENCE:direct assay:GO:0061499 outer plaque of mitotic spindle pole body [PMID:9585415]
EXISTENCE:direct assay:GO:0099609 microtubule lateral binding [PMID:9382872]
EXISTENCE:direct assay:GO:1905721 mitotic spindle astral microtubule end [PMID:12970190]
EXISTENCE:genetic interaction:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:17967947]
EXISTENCE:mutant phenotype:GO:0007020 microtubule nucleation [PMID:18573877]
EXISTENCE:mutant phenotype:GO:0030953 astral microtubule organization [PMID:12970190]
EXISTENCE:mutant phenotype:GO:0031122 cytoplasmic microtubule organization [PMID:9606209]
EXISTENCE:mutant phenotype:GO:1990734 astral microtubule anchoring at mitotic spindle pole body [PMID:12970190]
EXISTENCE:physical interaction:GO:0031578 mitotic spindle orientation checkpoint signaling [PMID:17967947]
EXISTENCE:physical interaction:GO:0110121 gamma-tubulin complex localization to cytoplasmic side of mitotic spindle pole body [PMID:9670012]
Gamma-tubulin small complex (gamma-TuSC) receptor; recruits the gamma-TuSC complex to the cytoplasmic side of the SPB, connecting nuclear microtubules to the SPB; involved in astral microtubule formation, stabilization, and with Stu2p, anchoring astral MTs at the cytoplasmic face of the SPB, and regulating plus-end MT dynamics; regulated by Cdc5 kinase
EXISTENCE:direct assay:GO:0005759 mitochondrial matrix [PMID:27532772]
EXISTENCE:genetic interaction:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:27532772|PMID:27532773]
EXISTENCE:mutant phenotype:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:27532772]
EXISTENCE:physical interaction:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:27532773]
Protein involved in Fe-S cluster transfer to mitochondrial clients; protects [4Fe-4S] clusters from damage due to oxidative stress by acting along with Nfu1p at a late step in the transfer of [4Fe-4S] clusters from the ISA complex to mitochondrial client proteins like lipoate synthase and succinate dehydrogenase; sequence similarity to human BOLA family member, BOLA3, mutations of which are associated with Multiple Mitochondria Dysfunctions Syndrome (MMDS2)
EXISTENCE:genetic interaction:GO:0044572 [4Fe-4S] cluster assembly [PMID:27532773]
EXISTENCE:genetic interaction:GO:0106035 protein maturation by [4Fe-4S] cluster transfer [PMID:27532772]
EXISTENCE:mutant phenotype:GO:0044572 [4Fe-4S] cluster assembly [PMID:27532773]
EXISTENCE:physical interaction:GO:0044572 [4Fe-4S] cluster assembly [PMID:27532773]
Mitochondrial matrix protein involved in Fe-S cluster biogenesis; facilitates [4Fe-2S] cluster inception into mitochondrial proteins such as lipoate synthase and succinate dehydrogenase; interacts and may function with Grx5p at an early step in Fe-S cluster biosynthesis; forms dimeric complexes with Grx5p and Nfu1p that alter the stability of shared Fe/S clusters; sequence similarity to human BOLA family member, BOLA1 and S. pombe uvi31, a putative DNA repair protein
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:19570983|PMID:14576278|PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0031405 lipoic acid binding [PMID:32974306]
EXISTENCE:genetic interaction:GO:0006730 one-carbon metabolic process [PMID:10871621]
EXISTENCE:mutant phenotype:GO:0005960 glycine cleavage complex [PMID:9020168]
EXISTENCE:mutant phenotype:GO:0006546 glycine catabolic process [PMID:9020168]
EXISTENCE:mutant phenotype:GO:0009249 protein lipoylation [PMID:19570983]
H subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF
EXISTENCE:curator inference:GO:0003723 RNA binding [PMID:10523662]
EXISTENCE:direct assay:GO:0005847 mRNA cleavage and polyadenylation specificity factor complex [PMID:10523662|PMID:12819204|PMID:9099738]
EXISTENCE:mutant phenotype:GO:0006378 mRNA polyadenylation [PMID:10523662|PMID:9303317]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:1508188]
EXISTENCE:mutant phenotype:GO:0098789 pre-mRNA cleavage required for polyadenylation [PMID:10523662]
EXISTENCE:physical interaction:GO:0030846 termination of RNA polymerase II transcription, poly(A)-coupled [PMID:12819204]
Subunit of holo-CPF; holo-CPF is a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII
EXISTENCE:direct assay:GO:0000139 Golgi membrane [PMID:11157978]
EXISTENCE:direct assay:GO:0005789 endoplasmic reticulum membrane [PMID:11157978]
EXISTENCE:direct assay:GO:0030134 COPII-coated ER to Golgi transport vesicle [PMID:11157978]
EXISTENCE:genetic interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:11157978]
Protein localized to COPII-coated vesicles; forms a complex with Erv41p; involved in the membrane fusion stage of transport
Rho family guanine nucleotide exchange factor CDC24
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:10655592]
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:8300560]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10655592|PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:10655592|PMID:22842922]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:10655592]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:10087263]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:10087263]
EXISTENCE:direct assay:GO:0120171 Cdc24p-Far1p-Gbetagamma complex [PMID:10087263]
EXISTENCE:genetic interaction:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:7565673]
EXISTENCE:mutant phenotype:GO:0000282 cellular bud site selection [PMID:7019215]
EXISTENCE:mutant phenotype:GO:0000750 pheromone-dependent signal transduction involved in conjugation with cellular fusion [PMID:7565673]
EXISTENCE:mutant phenotype:GO:0007096 regulation of exit from mitosis [PMID:12234925]
EXISTENCE:mutant phenotype:GO:0031106 septin ring organization [PMID:16371506]
EXISTENCE:mutant phenotype:GO:0043577 chemotropism [PMID:9428768]
EXISTENCE:mutant phenotype:GO:0072697 protein localization to cell cortex [PMID:16325503]
EXISTENCE:physical interaction:GO:0120171 Cdc24p-Far1p-Gbetagamma complex [PMID:10087263]
Guanine nucleotide exchange factor (GEF) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress; thermosensitivity of the cdc24-4 mutant in the presence of sorbitol is functionally complemented by human CDC42
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:11509671|PMID:11792824|PMID:10611233]
EXISTENCE:direct assay:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:1316273]
EXISTENCE:direct assay:GO:0042144 vacuole fusion, non-autophagic [PMID:14573462]
EXISTENCE:genetic interaction:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:19823668]
EXISTENCE:genetic interaction:GO:0007089 traversing start control point of mitotic cell cycle [PMID:2147225|PMID:2574633]
EXISTENCE:mutant phenotype:GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity [PMID:1316273|PMID:8387915]
EXISTENCE:mutant phenotype:GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle [PMID:19823668]
EXISTENCE:mutant phenotype:GO:0005634 nucleus [PMID:11509671]
EXISTENCE:mutant phenotype:GO:0007033 vacuole organization [PMID:14573462]
EXISTENCE:mutant phenotype:GO:0007089 traversing start control point of mitotic cell cycle [PMID:2907481|PMID:3062366]
EXISTENCE:mutant phenotype:GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity [PMID:1316273|PMID:8387915]
EXISTENCE:mutant phenotype:GO:0042144 vacuole fusion, non-autophagic [PMID:14573462]
G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote G1 to S phase transition; plays a role in regulating transcription of other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis; acetyl-CoA induces CLN3 transcription in response to nutrient repletion to promote cell-cycle entry; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1
Upstream open reading frame (uORF) in 5' untranslated region of CLN3 gene, regulate translation
EXISTENCE:direct assay:GO:0004408 holocytochrome-c synthase activity [PMID:3034577]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:24769239]
EXISTENCE:direct assay:GO:0005758 mitochondrial intermembrane space [PMID:1656231]
EXISTENCE:direct assay:GO:0018063 cytochrome c-heme linkage [PMID:3034577]
Cytochrome c heme lyase (holocytochrome c synthase); attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in mitochondrial intermembrane space; human homolog HCCS implicated in microphthalmia with linear skin defects (MLS), and can complement yeast null mutant
ARS106; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0004743 pyruvate kinase activity [PMID:12063246]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14718557|PMID:27385335]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:16622836]
EXISTENCE:mutant phenotype:GO:0004743 pyruvate kinase activity [PMID:323230]
EXISTENCE:mutant phenotype:GO:0006090 pyruvate metabolic process [PMID:323230]
EXISTENCE:mutant phenotype:GO:0006096 glycolytic process [PMID:323230]
Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
hypothetical protein; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:23002146]
EXISTENCE:direct assay:GO:0005777 peroxisome [PMID:36164978]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:19448108|PMID:23002146]
EXISTENCE:direct assay:GO:0010494 cytoplasmic stress granule [PMID:26777405]
EXISTENCE:genetic interaction:GO:0002181 cytoplasmic translation [PMID:21076151]
EXISTENCE:genetic interaction:GO:1903833 positive regulation of cellular response to amino acid starvation [PMID:23899355]
Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid; localizes to cytosol and peroxisomes
EXISTENCE:direct assay:GO:0001732 formation of cytoplasmic translation initiation complex [PMID:12008673]
EXISTENCE:direct assay:GO:0003743 translation initiation factor activity [PMID:12008673|PMID:9624054]
EXISTENCE:direct assay:GO:0003924 GTPase activity [PMID:19029250|PMID:16461768|PMID:17189426|PMID:12507428]
EXISTENCE:direct assay:GO:0005525 GTP binding [PMID:21335519|PMID:17189426]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22842922]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]
EXISTENCE:direct assay:GO:0022627 cytosolic small ribosomal subunit [PMID:9624054]
EXISTENCE:direct assay:GO:0031369 translation initiation factor binding [PMID:10982835]
EXISTENCE:direct assay:GO:0033290 eukaryotic 48S preinitiation complex [PMID:17242201]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:21335519|PMID:12507428]
EXISTENCE:direct assay:GO:0070181 small ribosomal subunit rRNA binding [PMID:19029250]
EXISTENCE:mutant phenotype:GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:22751017]
EXISTENCE:mutant phenotype:GO:0001732 formation of cytoplasmic translation initiation complex [PMID:17242201|PMID:12507428|PMID:17189426]
EXISTENCE:mutant phenotype:GO:0003924 GTPase activity [PMID:12471154]
EXISTENCE:mutant phenotype:GO:0006446 regulation of translational initiation [PMID:12507428]
EXISTENCE:mutant phenotype:GO:0042255 ribosome assembly [PMID:22770215]
EXISTENCE:mutant phenotype:GO:0042256 cytosolic ribosome assembly [PMID:12507428]
Translation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2
EXISTENCE:direct assay:GO:0000444 MIS12/MIND type complex [PMID:14633972]
EXISTENCE:direct assay:GO:0000922 spindle pole [PMID:12455957]
EXISTENCE:direct assay:GO:0007059 chromosome segregation [PMID:12455957]
EXISTENCE:mutant phenotype:GO:0034501 protein localization to kinetochore [PMID:19893618]
Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)
Non-essential hypothetical protein; expression induced in response to heat stress; FUN19 has a paralog, YOR338W, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000171 ribonuclease MRP activity [PMID:9620854]
EXISTENCE:direct assay:GO:0000172 ribonuclease MRP complex [PMID:9620854]
EXISTENCE:direct assay:GO:0000294 nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay [PMID:14729943]
EXISTENCE:direct assay:GO:0003723 RNA binding [PMID:23700311]
EXISTENCE:direct assay:GO:0004526 ribonuclease P activity [PMID:9620854]
EXISTENCE:direct assay:GO:0005655 nucleolar ribonuclease P complex [PMID:9620854]
EXISTENCE:direct assay:GO:0034965 intronic box C/D RNA processing [PMID:18713869]
EXISTENCE:mutant phenotype:GO:0000171 ribonuclease MRP activity [PMID:9620854]
EXISTENCE:mutant phenotype:GO:0004526 ribonuclease P activity [PMID:9620854]
EXISTENCE:mutant phenotype:GO:0006364 rRNA processing [PMID:9620854]
EXISTENCE:mutant phenotype:GO:0008033 tRNA processing [PMID:9620854]
EXISTENCE:physical interaction:GO:0000172 ribonuclease MRP complex [PMID:17881380]
Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
EXISTENCE:curator inference:GO:0000384 first spliceosomal transesterification activity [PMID:12554883]
EXISTENCE:curator inference:GO:0000386 second spliceosomal transesterification activity [PMID:12554883]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12359070]
EXISTENCE:direct assay:GO:0071006 U2-type catalytic step 1 spliceosome [PMID:12554883]
EXISTENCE:direct assay:GO:0071007 U2-type catalytic step 2 spliceosome [PMID:12554883]
EXISTENCE:direct assay:GO:0071014 post-mRNA release spliceosomal complex [PMID:12554883]
EXISTENCE:genetic interaction:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:19016306]
EXISTENCE:mutant phenotype:GO:0000350 generation of catalytic spliceosome for second transesterification step [PMID:19016306]
Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
EXISTENCE:direct assay:GO:0008157 protein phosphatase 1 binding [PMID:16537909]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:16537909]
EXISTENCE:mutant phenotype:GO:0019888 protein phosphatase regulator activity [PMID:16537909]
EXISTENCE:mutant phenotype:GO:0043666 regulation of phosphoprotein phosphatase activity [PMID:16537909]
Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; protein overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:14981247]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:16452633]
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:16452633|PMID:36125415]
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:10637288|PMID:36125415]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:26928762]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:20059749]
EXISTENCE:direct assay:GO:0006906 vesicle fusion [PMID:11001046]
EXISTENCE:direct assay:GO:0030658 transport vesicle membrane [PMID:10637288]
EXISTENCE:direct assay:GO:0031201 SNARE complex [PMID:9195974]
EXISTENCE:mutant phenotype:GO:0006887 exocytosis [PMID:9195971]
EXISTENCE:mutant phenotype:GO:0006893 Golgi to plasma membrane transport [PMID:8374953]
EXISTENCE:mutant phenotype:GO:0006897 endocytosis [PMID:11029060]
Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0000146 microfilament motor activity [PMID:11381095]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:16823961|PMID:14576278]
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:12725728|PMID:20439999]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:20439999]
EXISTENCE:direct assay:GO:0031941 filamentous actin [PMID:11381095]
EXISTENCE:direct assay:GO:0051015 actin filament binding [PMID:11381095]
EXISTENCE:mutant phenotype:GO:0007533 mating type switching [PMID:8625407]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:11030653|PMID:9219698|PMID:9288973]
EXISTENCE:mutant phenotype:GO:0048309 endoplasmic reticulum inheritance [PMID:14691136|PMID:16890529]
EXISTENCE:physical interaction:GO:0008298 intracellular mRNA localization [PMID:10212145|PMID:11030653]
Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:15189990]
EXISTENCE:mutant phenotype:GO:0071469 cellular response to alkaline pH [PMID:15189990]
EXISTENCE:mutant phenotype:GO:0071472 cellular response to salt stress [PMID:15189990]
EXISTENCE:physical interaction:GO:0031204 post-translational protein targeting to membrane, translocation [PMID:21097665]
Tail-anchored ER membrane hypothetical protein; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0032079 positive regulation of endodeoxyribonuclease activity [PMID:23299942]
EXISTENCE:direct assay:GO:0070337 3'-flap-structured DNA binding [PMID:23299942]
EXISTENCE:direct assay:GO:0070338 5'-flap-structured DNA binding [PMID:23299942]
EXISTENCE:genetic interaction:GO:0000736 double-strand break repair via single-strand annealing, removal of nonhomologous ends [PMID:18471978]
EXISTENCE:mutant phenotype:GO:0000736 double-strand break repair via single-strand annealing, removal of nonhomologous ends [PMID:18471978]
5'- and 3'-flap DNA binding protein; required for recruitment of Rad1p-Rad10p to single-strand annealing intermediates with 3' non-homologous tails for removal during double-strand break repair; forms a complex with Rad1p-Rad10p and stimulates its endonuclease activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
aminophospholipid-translocating P4-type ATPase DRS2
EXISTENCE:direct assay:GO:0005802 trans-Golgi network [PMID:12221123|PMID:15090616|PMID:10601336]
EXISTENCE:genetic interaction:GO:0000749 response to pheromone triggering conjugation with cellular fusion [PMID:25378585]
EXISTENCE:genetic interaction:GO:0006886 intracellular protein transport [PMID:12221123]
EXISTENCE:genetic interaction:GO:0006892 post-Golgi vesicle-mediated transport [PMID:10601336]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:12631737|PMID:16195350]
EXISTENCE:genetic interaction:GO:0090555 phosphatidylethanolamine flippase activity [PMID:16452632]
EXISTENCE:genetic interaction:GO:0140345 phosphatidylcholine flippase activity [PMID:16452632]
EXISTENCE:genetic interaction:GO:0140346 phosphatidylserine flippase activity [PMID:16452632]
EXISTENCE:mutant phenotype:GO:0006892 post-Golgi vesicle-mediated transport [PMID:10601336]
EXISTENCE:mutant phenotype:GO:0032456 endocytic recycling [PMID:24272750]
EXISTENCE:mutant phenotype:GO:0045332 phospholipid translocation [PMID:15249668|PMID:16452632]
EXISTENCE:mutant phenotype:GO:0090555 phosphatidylethanolamine flippase activity [PMID:16452632]
EXISTENCE:mutant phenotype:GO:0140346 phosphatidylserine flippase activity [PMID:8633245|PMID:15249668|PMID:16452632]
EXISTENCE:physical interaction:GO:1990530 Cdc50p-Drs2p complex [PMID:15090616]
Trans-golgi network aminophospholipid translocase (flippase); P4-type ATPase; maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation, endocytosis, protein trafficking between the Golgi and endosomal system and the cellular response to mating pheromone; autoinhibited by its C-terminal tail; localizes to the trans-Golgi network
Non-protein-coding RNA; substrate of RNase P, possibly involved in rRNA processing, specifically maturation of 20S precursor into the mature 18S rRNA
EXISTENCE:direct assay:GO:0005730 nucleolus [PMID:10838225]
EXISTENCE:direct assay:GO:0030687 preribosome, large subunit precursor [PMID:11583614]
EXISTENCE:mutant phenotype:GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16710831]
EXISTENCE:mutant phenotype:GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16710831]
EXISTENCE:mutant phenotype:GO:0042273 ribosomal large subunit biogenesis [PMID:7739558]
Essential nuclear protein; constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus
EXISTENCE:direct assay:GO:0005933 cellular bud [PMID:12782684]
EXISTENCE:genetic interaction:GO:0016192 vesicle-mediated transport [PMID:17121813]
EXISTENCE:mutant phenotype:GO:0031536 positive regulation of exit from mitosis [PMID:27798238]
EXISTENCE:mutant phenotype:GO:0061510 asymmetric protein localization to new mitotic spindle pole body [PMID:19948498]
Protein similar to GDP/GTP exchange factors; without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
dolichyl-phosphate-mannose-protein mannosyltransferase PMT2
EXISTENCE:direct assay:GO:0004169 dolichyl-phosphate-mannose-protein mannosyltransferase activity [PMID:21956107]
EXISTENCE:direct assay:GO:0097582 dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt2p dimer complex [PMID:12551906]
EXISTENCE:direct assay:GO:0097584 dolichyl-phosphate-mannose-protein mannosyltransferase Pmt5p-Pmt2p dimer complex [PMID:12551906]
EXISTENCE:genetic interaction:GO:0004169 dolichyl-phosphate-mannose-protein mannosyltransferase activity [PMID:21956107|PMID:7852348|PMID:8543034]
EXISTENCE:genetic interaction:GO:0032527 protein exit from endoplasmic reticulum [PMID:21147851]
EXISTENCE:genetic interaction:GO:0035269 protein O-linked mannosylation [PMID:21231968|PMID:7852348|PMID:8543034]
EXISTENCE:genetic interaction:GO:0071712 ER-associated misfolded protein catabolic process [PMID:21147851]
EXISTENCE:genetic interaction:GO:1900101 regulation of endoplasmic reticulum unfolded protein response [PMID:21231968|PMID:23704572]
EXISTENCE:mutant phenotype:GO:0004169 dolichyl-phosphate-mannose-protein mannosyltransferase activity [PMID:7852348|PMID:8543034]
EXISTENCE:mutant phenotype:GO:0006493 protein O-linked glycosylation [PMID:9184828]
EXISTENCE:mutant phenotype:GO:0035269 protein O-linked mannosylation [PMID:7852348|PMID:8543034|PMID:18182384]
EXISTENCE:mutant phenotype:GO:0071712 ER-associated misfolded protein catabolic process [PMID:18182384]
EXISTENCE:mutant phenotype:GO:1900101 regulation of endoplasmic reticulum unfolded protein response [PMID:23704572]
EXISTENCE:physical interaction:GO:0097582 dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt2p dimer complex [PMID:21956107|PMID:8543034]
Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; functions as a heterodimer with Pmt2p but can also pair with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication
nucleoside transmembrane transporter FUN26
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:21349851|PMID:19001347|PMID:26928762]
EXISTENCE:direct assay:GO:0005337 nucleoside transmembrane transporter activity [PMID:10827169|PMID:25035431]
EXISTENCE:direct assay:GO:0015205 nucleobase transmembrane transporter activity [PMID:25035431]
EXISTENCE:direct assay:GO:0015851 nucleobase transport [PMID:25035431]
EXISTENCE:direct assay:GO:0015858 nucleoside transport [PMID:10827169|PMID:25035431]
EXISTENCE:direct assay:GO:0055085 transmembrane transport [PMID:10827169]
EXISTENCE:mutant phenotype:GO:0034257 nicotinamide riboside transmembrane transporter activity [PMID:21349851]
EXISTENCE:mutant phenotype:GO:0034258 nicotinamide riboside transport [PMID:21349851]
High affinity, broad selectivity, nucleoside/nucleobase transporter; vacuolar membrane localized transporter which may regulate the balance of nicotinamide riboside (NmR) levels between the cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis; equilibrative nucleoside transporter (ENT) family member
CCR4-NOT core exoribonuclease subunit CCR4
EXISTENCE:direct assay:GO:0000175 3'-5'-exoribonuclease activity [PMID:11889048]
EXISTENCE:direct assay:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:11889048]
EXISTENCE:direct assay:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:11889048]
EXISTENCE:direct assay:GO:0000932 P-body [PMID:18611963]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:11889048]
EXISTENCE:direct assay:GO:0030015 CCR4-NOT core complex [PMID:10490603]
EXISTENCE:direct assay:GO:0032968 positive regulation of transcription elongation by RNA polymerase II [PMID:21406554]
EXISTENCE:genetic interaction:GO:0000076 DNA replication checkpoint signaling [PMID:17158920]
EXISTENCE:genetic interaction:GO:0000175 3'-5'-exoribonuclease activity [PMID:11889048]
EXISTENCE:genetic interaction:GO:0006260 DNA replication [PMID:17158920]
EXISTENCE:genetic interaction:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11404327]
EXISTENCE:genetic interaction:GO:0048478 replication fork protection [PMID:17158920]
EXISTENCE:mutant phenotype:GO:0000175 3'-5'-exoribonuclease activity [PMID:11239395|PMID:11889048]
EXISTENCE:mutant phenotype:GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [PMID:11889048]
EXISTENCE:mutant phenotype:GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening [PMID:11410650|PMID:17158920]
EXISTENCE:mutant phenotype:GO:0006368 transcription elongation by RNA polymerase II promoter [PMID:11404327]
EXISTENCE:mutant phenotype:GO:0007089 traversing start control point of mitotic cell cycle [PMID:15075273]
EXISTENCE:physical interaction:GO:0006357 regulation of transcription by RNA polymerase II [PMID:10490603]
EXISTENCE:physical interaction:GO:0016593 Cdc73/Paf1 complex [PMID:9891041]
Component of the CCR4-NOT transcriptional complex; CCR4-NOT is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:18466297]
EXISTENCE:mutant phenotype:GO:0002098 tRNA wobble uridine modification [PMID:18755837]
Protein required for modification of wobble nucleosides in tRNA; acts with Elongator complex, Kti11p, and Kti12p; has a potential role in regulatory interactions between microtubules and the cell cycle; forms a stable heterodimer with Kti11p
EXISTENCE:direct assay:GO:0000775 chromosome, centromeric region [PMID:23028372]
EXISTENCE:direct assay:GO:0003677 DNA binding [PMID:20075079]
EXISTENCE:direct assay:GO:0003682 chromatin binding [PMID:20075079]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0031934 mating-type region heterochromatin [PMID:19956593]
EXISTENCE:direct assay:GO:0140658 ATP-dependent chromatin remodeler activity [PMID:20075079|PMID:23779104]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:23779104]
EXISTENCE:mutant phenotype:GO:0000183 rDNA heterochromatin formation [PMID:19956593]
EXISTENCE:mutant phenotype:GO:0000729 DNA double-strand break processing [PMID:22960743|PMID:22960744]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:23779104|PMID:33141948|PMID:20075079]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:19956593]
EXISTENCE:mutant phenotype:GO:0031507 heterochromatin formation [PMID:21388962]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:19956593]
EXISTENCE:mutant phenotype:GO:0033120 positive regulation of RNA splicing [PMID:32234239]
EXISTENCE:mutant phenotype:GO:0140658 ATP-dependent chromatin remodeler activity [PMID:19956593]
Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate
EXISTENCE:direct assay:GO:0005619 ascospore wall [PMID:23966878]
EXISTENCE:direct assay:GO:0005628 prospore membrane [PMID:23966878]
EXISTENCE:direct assay:GO:0005811 lipid droplet [PMID:24390141]
EXISTENCE:genetic interaction:GO:0030476 ascospore wall assembly [PMID:23966878]
Protein Involved in spore wall assembly; localizes to lipid droplets found on or outside of the prospore membrane; shares similarity with Lds2p and Rrt8p, and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants
EXISTENCE:direct assay:GO:0004672 protein kinase activity [PMID:16319894]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:22289182|PMID:14562095]
EXISTENCE:genetic interaction:GO:0060917 regulation of (1->6)-beta-D-glucan biosynthetic process [PMID:17531808]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:12372297]
EXISTENCE:mutant phenotype:GO:0045719 negative regulation of glycogen biosynthetic process [PMID:12372297]
PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication
ARS107; Autonomously Replicating Sequence
protein phosphatase 2A structural subunit TPD3
EXISTENCE:direct assay:GO:0000159 protein phosphatase type 2A complex [PMID:17550305|PMID:8600023]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:12388751]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:12388751]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:12388751]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:12388751]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12388751]
EXISTENCE:direct assay:GO:0043332 mating projection tip [PMID:12388751]
EXISTENCE:mutant phenotype:GO:0004722 protein serine/threonine phosphatase activity [PMID:17550305]
EXISTENCE:mutant phenotype:GO:0006417 regulation of translation [PMID:10329624]
EXISTENCE:mutant phenotype:GO:0007094 mitotic spindle assembly checkpoint signaling [PMID:9001215]
Regulatory subunit A of the heterotrimeric PP2A complex; the heterotrimeric protein phosphatase 2A (PP2A) complex also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and transcription by RNA polymerase III
bifunctional N-glycosylase/AP lyase NTG1
EXISTENCE:direct assay:GO:0000703 oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity [PMID:9020769]
EXISTENCE:direct assay:GO:0003906 DNA-(apurinic or apyrimidinic site) endonuclease activity [PMID:14500818]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10207101|PMID:19029246|PMID:24034606]
EXISTENCE:direct assay:GO:0006281 DNA repair [PMID:8855249]
EXISTENCE:direct assay:GO:0006284 base-excision repair [PMID:14500818]
EXISTENCE:direct assay:GO:0006285 base-excision repair, AP site formation [PMID:9826748]
EXISTENCE:direct assay:GO:0008534 oxidized purine nucleobase lesion DNA N-glycosylase activity [PMID:8855249]
EXISTENCE:mutant phenotype:GO:0034599 cellular response to oxidative stress [PMID:19074198]
EXISTENCE:mutant phenotype:GO:0090297 positive regulation of mitochondrial DNA replication [PMID:17116696|PMID:19074198]
DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; required for maintaining mitochondrial genome integrity; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0005484 SNAP receptor activity [PMID:12453154]
EXISTENCE:direct assay:GO:0005768 endosome [PMID:12453154]
EXISTENCE:mutant phenotype:GO:0006896 Golgi to vacuole transport [PMID:12453154]
EXISTENCE:physical interaction:GO:0006896 Golgi to vacuole transport [PMID:12453154]
Endosomal SNARE related to mammalian syntaxin 8
Rpd3L histone deacetylase complex subunit DEP1
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:36283334]
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:36283334]
EXISTENCE:direct assay:GO:0070210 Rpd3L-Expanded complex [PMID:19040720]
EXISTENCE:mutant phenotype:GO:0000122 negative regulation of transcription by RNA polymerase II [PMID:16314178|PMID:24358376|PMID:8056324]
EXISTENCE:mutant phenotype:GO:0006357 regulation of transcription by RNA polymerase II [PMID:21646424|PMID:8056324]
EXISTENCE:mutant phenotype:GO:0030174 regulation of DNA-templated DNA replication initiation [PMID:19417103]
EXISTENCE:mutant phenotype:GO:0045944 positive regulation of transcription by RNA polymerase II [PMID:20398213|PMID:8056324]
EXISTENCE:mutant phenotype:GO:0061186 negative regulation of silent mating-type cassette heterochromatin formation [PMID:16286008]
EXISTENCE:mutant phenotype:GO:0061188 negative regulation of rDNA heterochromatin formation [PMID:16286008]
Component of the Rpd3L histone deacetylase complex; required for diauxic shift-induced histone H2B deposition onto rDNA genes; transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation; localizes to both the nucleus and mitochondria where it may play a role in mitophagy
EXISTENCE:direct assay:GO:0004123 cystathionine gamma-lyase activity [PMID:1577698]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22842922]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:14562095|PMID:22842922]
EXISTENCE:direct assay:GO:0047804 cysteine-S-conjugate beta-lyase activity [PMID:29436228]
EXISTENCE:direct assay:GO:1904828 positive regulation of hydrogen sulfide biosynthetic process [PMID:31582588]
EXISTENCE:mutant phenotype:GO:0004123 cystathionine gamma-lyase activity [PMID:10509018|PMID:8366024]
EXISTENCE:mutant phenotype:GO:0019343 cysteine biosynthetic process via cystathionine [PMID:10509018]
EXISTENCE:mutant phenotype:GO:0019344 cysteine biosynthetic process [PMID:8366024]
EXISTENCE:mutant phenotype:GO:0019346 transsulfuration [PMID:8366024]
Cystathionine gamma-lyase; catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0000812 Swr1 complex [PMID:14645854|PMID:14690608]
EXISTENCE:direct assay:GO:0006338 chromatin remodeling [PMID:14645854]
EXISTENCE:mutant phenotype:GO:0006338 chromatin remodeling [PMID:14690608]
EXISTENCE:mutant phenotype:GO:0007029 endoplasmic reticulum organization [PMID:12868057]
hypothetical protein; component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:16407407]
EXISTENCE:genetic interaction:GO:0015914 phospholipid transport [PMID:19556461]
EXISTENCE:mutant phenotype:GO:0007005 mitochondrion organization [PMID:19556461]
EXISTENCE:mutant phenotype:GO:0007031 peroxisome organization [PMID:30908556]
EXISTENCE:mutant phenotype:GO:0045040 protein insertion into mitochondrial outer membrane [PMID:17410204]
EXISTENCE:mutant phenotype:GO:0051654 establishment of mitochondrion localization [PMID:13679517]
EXISTENCE:mutant phenotype:GO:0070096 mitochondrial outer membrane translocase complex assembly [PMID:20026336|PMID:15239954]
EXISTENCE:mutant phenotype:GO:1990456 mitochondrion-endoplasmic reticulum membrane tethering [PMID:19556461]
EXISTENCE:physical interaction:GO:0001401 SAM complex [PMID:17410204]
EXISTENCE:physical interaction:GO:0032865 ERMES complex [PMID:13679517|PMID:17410204]
EXISTENCE:physical interaction:GO:0070096 mitochondrial outer membrane translocase complex assembly [PMID:20026336]
Subunit of both the ERMES and the SAM complex; component of ERMES complex which acts as a molecular tether between the mitochondria and the ER, necessary for efficient phospholipid exchange between organelles and for mitophagy; SAM/TOB complex component that functions in the assembly of outer membrane beta-barrel proteins; involved in mitochondrial inheritance and morphology; ERMES complex is often co-localized with peroxisomes and concentrated areas of pyruvate dehydrogenase
Nem1-Spo7 phosphatase regulatory subunit SPO7
EXISTENCE:direct assay:GO:0004721 phosphoprotein phosphatase activity [PMID:15889145|PMID:25359770]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:9822591]
EXISTENCE:direct assay:GO:0061709 reticulophagy [PMID:31802527]
EXISTENCE:direct assay:GO:1903740 positive regulation of phosphatidate phosphatase activity [PMID:25359770]
EXISTENCE:genetic interaction:GO:0071072 negative regulation of phospholipid biosynthetic process [PMID:15889145]
EXISTENCE:mutant phenotype:GO:0006998 nuclear envelope organization [PMID:9822591]
EXISTENCE:mutant phenotype:GO:0071071 regulation of phospholipid biosynthetic process [PMID:30201607]
EXISTENCE:physical interaction:GO:0071595 Nem1-Spo7 phosphatase complex [PMID:9822591]
Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear/ER membrane association and activation of Pah1p, a phosphatidate phosphatase involved in the production of diacylglycerol DAG for lipid droplet biogenesis; phosphatase activity of the Nem1p-Spo7p complex is inhibited by Ice2p; regulates nuclear growth by controlling phospholipid biosynthesis; required for normal nuclear envelope morphology, premeiotic replication, and sporulation
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:14562095|PMID:24769239|PMID:14576278|PMID:16823961]
EXISTENCE:direct assay:GO:0005741 mitochondrial outer membrane [PMID:27226123|PMID:16407407]
EXISTENCE:genetic interaction:GO:0007005 mitochondrion organization [PMID:27226123]
EXISTENCE:genetic interaction:GO:0055091 phospholipid homeostasis [PMID:27226123]
Integral mitochondrial outer membrane (MOM) protein; dosage suppressor of an MDM10 null that reduces ERMES-related phenotypes, such as alterations in mitochondrial morphology, protein complex assembly, and lipid profile; dosage suppressor of MDM12, MDM34, and MMM1 null mutant growth defects; novel mechanism of MOM import involving Tom70p, the TOM complex, and the TIM23 complex, requiring mitochondrial membrane potential and processing by the IMP complex for correct biogenesis
EXISTENCE:mutant phenotype:GO:0006621 protein retention in ER lumen [PMID:10359606]
EXISTENCE:mutant phenotype:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:10359606]
Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP2 has a paralog, ERP4, that arose from the whole genome duplication
EXISTENCE:curator inference:GO:0005829 cytosol [PMID:9023104]
EXISTENCE:curator inference:GO:0006414 translational elongation [PMID:9023104]
Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE analysis; target of K. lactis zymocin; can mutate to suppress +1 frameshift mutations in proline codons
EXISTENCE:direct assay:GO:0000049 tRNA binding [PMID:25853343]
EXISTENCE:direct assay:GO:0000209 protein polyubiquitination [PMID:20462952]
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:10745074]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:10347213|PMID:11914276]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:8755907|PMID:11914276]
EXISTENCE:direct assay:GO:0005844 polysome [PMID:16413483]
EXISTENCE:direct assay:GO:0006457 protein folding [PMID:8947547]
EXISTENCE:direct assay:GO:0006606 protein import into nucleus [PMID:10347213]
EXISTENCE:direct assay:GO:0006616 SRP-dependent cotranslational protein targeting to membrane, translocation [PMID:8754838]
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:8755907]
EXISTENCE:direct assay:GO:0016887 ATP hydrolysis activity [PMID:7737974|PMID:18706386]
EXISTENCE:direct assay:GO:0035617 stress granule disassembly [PMID:24291094]
EXISTENCE:direct assay:GO:0042026 protein refolding [PMID:18706386|PMID:9674429]
EXISTENCE:direct assay:GO:0051082 unfolded protein binding [PMID:9789005]
EXISTENCE:direct assay:GO:0072318 clathrin coat disassembly [PMID:23913685]
EXISTENCE:genetic interaction:GO:0006606 protein import into nucleus [PMID:10347213]
EXISTENCE:genetic interaction:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:27178214]
EXISTENCE:mutant phenotype:GO:0002181 cytoplasmic translation [PMID:11279042]
EXISTENCE:mutant phenotype:GO:0006626 protein targeting to mitochondrion [PMID:8754838]
EXISTENCE:mutant phenotype:GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process [PMID:27178214]
EXISTENCE:mutant phenotype:GO:0072671 mitochondria-associated ubiquitin-dependent protein catabolic process [PMID:32118579]
ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; required for ubiquitin-dependent degradation of short-lived proteins; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, cell wall; 98% identical to paralog Ssa2p with different functional specificity in propagation of yeast [URE3] prions, vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils
translation elongation factor 1 subunit beta
EXISTENCE:direct assay:GO:0005085 guanyl-nucleotide exchange factor activity [PMID:17925388]
EXISTENCE:direct assay:GO:0032232 negative regulation of actin filament bundle assembly [PMID:19095653]
EXISTENCE:genetic interaction:GO:0006449 regulation of translational termination [PMID:19545407]
EXISTENCE:mutant phenotype:GO:0005853 eukaryotic translation elongation factor 1 complex [PMID:8420802]
EXISTENCE:mutant phenotype:GO:0006414 translational elongation [PMID:10409717]
EXISTENCE:mutant phenotype:GO:1990145 maintenance of translational fidelity [PMID:10409717]
Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site; human homolog EEF1B2 can complement yeast efb1 mutants
EXISTENCE:curator inference:GO:0005730 nucleolus [PMID:8674114]
EXISTENCE:direct assay:GO:0031428 box C/D RNP complex [PMID:10094313]
EXISTENCE:physical interaction:GO:0031428 box C/D RNP complex [PMID:10733567]
C/D box small nucleolar RNA (snoRNA); commonly referred to as U18; guides 2'-O-methylation of large subunit (LSU) rRNA at positions A649 and C650
CORVET complex membrane-binding subunit VPS8
EXISTENCE:direct assay:GO:0005770 late endosome [PMID:19828734]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:8969229]
EXISTENCE:direct assay:GO:0033263 CORVET complex [PMID:17488625]
EXISTENCE:direct assay:GO:0051020 GTPase binding [PMID:19828734]
EXISTENCE:mutant phenotype:GO:0006623 protein targeting to vacuole [PMID:20173035]
EXISTENCE:mutant phenotype:GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway [PMID:19828734]
EXISTENCE:mutant phenotype:GO:0043495 protein-membrane adaptor activity [PMID:19828734]
EXISTENCE:physical interaction:GO:0051020 GTPase binding [PMID:19828734]
Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
ARS108; Autonomously Replicating Sequence
transcription factor TFIIIC subunit TFC3
EXISTENCE:direct assay:GO:0000127 transcription factor TFIIIC complex [PMID:1279682|PMID:1474578|PMID:2002052|PMID:2180956]
EXISTENCE:direct assay:GO:0001002 RNA polymerase III type 1 promoter sequence-specific DNA binding [PMID:2404611|PMID:2649882]
EXISTENCE:direct assay:GO:0001003 RNA polymerase III type 2 promoter sequence-specific DNA binding [PMID:2404611]
EXISTENCE:direct assay:GO:0006383 transcription by RNA polymerase III [PMID:3633923|PMID:8464480|PMID:2183033]
EXISTENCE:direct assay:GO:0008301 DNA binding, bending [PMID:2002052]
EXISTENCE:direct assay:GO:0042791 5S class rRNA transcription by RNA polymerase III [PMID:2183033]
EXISTENCE:mutant phenotype:GO:0042791 5S class rRNA transcription by RNA polymerase III [PMID:8083243]
EXISTENCE:mutant phenotype:GO:0071168 protein localization to chromatin [PMID:18708580]
Subunit of RNA polymerase III transcription initiation factor complex; part of TauB domain of TFIIIC that binds DNA at BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding; largest of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); colocalizes with condensin at pol III genes and several ETC (extra TFIIIC) sites; may have a role in recruiting or stabilizing Scc2/4 and condensin on chromosomes
EXISTENCE:direct assay:GO:0005543 phospholipid binding [PMID:17897934]
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:22932476]
EXISTENCE:direct assay:GO:0005643 nuclear pore [PMID:10684247]
EXISTENCE:direct assay:GO:0005829 cytosol [PMID:22932476]
EXISTENCE:direct assay:GO:0044613 nuclear pore central transport channel [PMID:18046406]
EXISTENCE:direct assay:GO:0044615 nuclear pore nuclear basket [PMID:10684247]
EXISTENCE:genetic interaction:GO:0006607 NLS-bearing protein import into nucleus [PMID:15039779]
EXISTENCE:genetic interaction:GO:0017056 structural constituent of nuclear pore [PMID:11387327]
EXISTENCE:genetic interaction:GO:0060188 regulation of protein desumoylation [PMID:17403926]
EXISTENCE:mutant phenotype:GO:0000972 transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:20098417|PMID:20932479|PMID:21036941]
EXISTENCE:mutant phenotype:GO:0000973 post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery [PMID:20932479]
EXISTENCE:mutant phenotype:GO:0006302 double-strand break repair [PMID:17538013]
EXISTENCE:mutant phenotype:GO:0006607 NLS-bearing protein import into nucleus [PMID:11535617]
EXISTENCE:mutant phenotype:GO:0006611 protein export from nucleus [PMID:11535617]
EXISTENCE:mutant phenotype:GO:0006913 nucleocytoplasmic transport [PMID:11535617]
EXISTENCE:mutant phenotype:GO:0008298 intracellular mRNA localization [PMID:21036941]
EXISTENCE:mutant phenotype:GO:0016973 poly(A)+ mRNA export from nucleus [PMID:12411502|PMID:21036941]
EXISTENCE:mutant phenotype:GO:0017056 structural constituent of nuclear pore [PMID:17418788]
EXISTENCE:mutant phenotype:GO:0030466 silent mating-type cassette heterochromatin formation [PMID:21818277]
EXISTENCE:mutant phenotype:GO:0031990 mRNA export from nucleus in response to heat stress [PMID:18258809]
EXISTENCE:mutant phenotype:GO:0034398 telomere tethering at nuclear periphery [PMID:11862215|PMID:21818277]
EXISTENCE:mutant phenotype:GO:0051276 chromosome organization [PMID:21818277]
EXISTENCE:physical interaction:GO:0017056 structural constituent of nuclear pore [PMID:17418788]
FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; involved in gene tethering at the nuclear periphery; relocalizes to the cytosol in response to hypoxia; both NUP1 and NUP60 are homologous to human NUP153
EXISTENCE:physical interaction:GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport [PMID:11157978]
Member of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0042800 histone H3K4 methyltransferase activity [PMID:11742990|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0000723 telomere maintenance [PMID:11742990]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:11687631]
EXISTENCE:mutant phenotype:GO:0031509 subtelomeric heterochromatin formation [PMID:11687631|PMID:11752412|PMID:11805083]
EXISTENCE:mutant phenotype:GO:0042800 histone H3K4 methyltransferase activity [PMID:11752412|PMID:11805083]
EXISTENCE:physical interaction:GO:0048188 Set1C/COMPASS complex [PMID:11687631|PMID:11742990|PMID:11752412]
Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7
replication factor A subunit protein RFA1
EXISTENCE:direct assay:GO:0000794 condensed nuclear chromosome [PMID:9679065]
EXISTENCE:direct assay:GO:0003690 double-stranded DNA binding [PMID:7761422]
EXISTENCE:direct assay:GO:0003697 single-stranded DNA binding [PMID:8804316]
EXISTENCE:direct assay:GO:0003729 mRNA binding [PMID:23222640]
EXISTENCE:direct assay:GO:0005662 DNA replication factor A complex [PMID:2554144]
EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:15282802]
EXISTENCE:direct assay:GO:0006265 DNA topological change [PMID:22885009]
EXISTENCE:direct assay:GO:0006268 DNA unwinding involved in DNA replication [PMID:2554144]
EXISTENCE:direct assay:GO:0006289 nucleotide-excision repair [PMID:8910442]
EXISTENCE:direct assay:GO:0030491 heteroduplex formation [PMID:12226081]
EXISTENCE:direct assay:GO:0032079 positive regulation of endodeoxyribonuclease activity [PMID:34764291]
EXISTENCE:direct assay:GO:0043565 sequence-specific DNA binding [PMID:7761422]
EXISTENCE:genetic interaction:GO:0000722 telomere maintenance via recombination [PMID:17202155]
EXISTENCE:genetic interaction:GO:0000724 double-strand break repair via homologous recombination [PMID:9679065]
EXISTENCE:mutant phenotype:GO:0000781 chromosome, telomeric region [PMID:14702040]
EXISTENCE:mutant phenotype:GO:0006260 DNA replication [PMID:7969128]
EXISTENCE:mutant phenotype:GO:0006281 DNA repair [PMID:7969128]
EXISTENCE:mutant phenotype:GO:0006312 mitotic recombination [PMID:7969128]
EXISTENCE:mutant phenotype:GO:0007131 reciprocal meiotic recombination [PMID:12072452]
EXISTENCE:mutant phenotype:GO:0010833 telomere maintenance via telomere lengthening [PMID:22354040]
EXISTENCE:mutant phenotype:GO:0016567 protein ubiquitination [PMID:18342608]
EXISTENCE:mutant phenotype:GO:0032079 positive regulation of endodeoxyribonuclease activity [PMID:34764291]
EXISTENCE:mutant phenotype:GO:1905776 positive regulation of DNA helicase activity [PMID:34764291]
EXISTENCE:physical interaction:GO:0007004 telomere maintenance via telomerase [PMID:2554144]
EXISTENCE:physical interaction:GO:0016567 protein ubiquitination [PMID:18342608]
EXISTENCE:physical interaction:GO:0045184 establishment of protein localization [PMID:2554144]
Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved ssDNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; promotes recruitment, helicase and nuclease activities of Dna2p; role in DNA catenation/decatenation pathway of chromosome disentangling; relocalizes to the cytosol in response to hypoxia
tRNA splicing endonuclease subunit SEN34
EXISTENCE:direct assay:GO:0000213 tRNA-intron endonuclease activity [PMID:9200603]
EXISTENCE:direct assay:GO:0000214 tRNA-intron endonuclease complex [PMID:9200603]
EXISTENCE:direct assay:GO:0000379 tRNA-type intron splice site recognition and cleavage [PMID:9200603]
EXISTENCE:mutant phenotype:GO:0000213 tRNA-intron endonuclease activity [PMID:9200603]
EXISTENCE:mutant phenotype:GO:0000379 tRNA-type intron splice site recognition and cleavage [PMID:9200603]
Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease (Sen complex) is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the CBP1 mRNA at the mitochondrial surface; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
ARS101; ARS containing multiple redundant ORC binding sites; ORC is the acronym for origin recognition complex
YARCTy1-1; Ty1 element, LTR retrotransposon of the Copia (Pseudoviridae) group; contains genes TYA Gag and TYB Pol, encoding proteins involved in structure and function of virus-like particles, flanked by two direct repeats; mutated in S288C
EXISTENCE:direct assay:GO:0005634 nucleus [PMID:9448009]
Retrotransposon TYA Gag and TYB Pol genes; Gag processing produces capsid proteins, Pol is cleaved to produce protease, reverse transcriptase and integrase activities; in YARCTy1-1 TYB is mutant and probably non-functional; protein product forms cytoplasmic foci upon DNA replication stress
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; Gag processing produces capsid proteins; in YARCTy1-1 TYB is mutant and probably non-functional
Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE analysis; one of 5 nuclear tRNA genes containing the tDNA-anticodon TGC (mature tRNA may be UGC or may contain modified bases), decodes GCA and probably GCG codons into alanine, one of 16 nuclear tRNAs for alanine
EXISTENCE:direct assay:GO:0000131 incipient cellular bud site [PMID:11452010]
EXISTENCE:direct assay:GO:0005934 cellular bud tip [PMID:11452010]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:11452010]
EXISTENCE:direct assay:GO:0006355 regulation of DNA-templated transcription [PMID:15601868]
EXISTENCE:direct assay:GO:0019888 protein phosphatase regulator activity [PMID:16107882]
EXISTENCE:direct assay:GO:0030837 negative regulation of actin filament polymerization [PMID:19217430]
EXISTENCE:direct assay:GO:0032880 regulation of protein localization [PMID:19217430]
EXISTENCE:direct assay:GO:1990615 Kelch-containing formin regulatory complex [PMID:24828508]
EXISTENCE:genetic interaction:GO:0008360 regulation of cell shape [PMID:12477789]
EXISTENCE:genetic interaction:GO:0030837 negative regulation of actin filament polymerization [PMID:19217430]
EXISTENCE:genetic interaction:GO:0032465 regulation of cytokinesis [PMID:24828508]
EXISTENCE:genetic interaction:GO:0090337 regulation of formin-nucleated actin cable assembly [PMID:24828508]
EXISTENCE:mutant phenotype:GO:0001100 negative regulation of exit from mitosis [PMID:34633288]
EXISTENCE:mutant phenotype:GO:0006355 regulation of DNA-templated transcription [PMID:15601868]
EXISTENCE:mutant phenotype:GO:0007010 cytoskeleton organization [PMID:16107882]
EXISTENCE:mutant phenotype:GO:0019888 protein phosphatase regulator activity [PMID:16107882]
EXISTENCE:mutant phenotype:GO:0030837 negative regulation of actin filament polymerization [PMID:19217430]
EXISTENCE:mutant phenotype:GO:0060627 regulation of vesicle-mediated transport [PMID:24828508]
Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; forms a complex with Kel1p and Kel2p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress
phosphoribosylaminoimidazolesuccinocarboxamide synthase
EXISTENCE:direct assay:GO:0004639 phosphoribosylaminoimidazolesuccinocarboxamide synthase activity [PMID:1756975]
EXISTENCE:direct assay:GO:0006164 purine nucleotide biosynthetic process [PMID:1756975]
EXISTENCE:direct assay:GO:0006189 'de novo' IMP biosynthetic process [PMID:1756975]
EXISTENCE:mutant phenotype:GO:0004639 phosphoribosylaminoimidazolesuccinocarboxamide synthase activity [PMID:5767024|PMID:5767025]
EXISTENCE:mutant phenotype:GO:0006164 purine nucleotide biosynthetic process [PMID:5767025|PMID:5767024]
EXISTENCE:mutant phenotype:GO:0006189 'de novo' IMP biosynthetic process [PMID:5767024|PMID:5767025]
N-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetase; required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress
EXISTENCE:direct assay:GO:0006468 protein phosphorylation [PMID:16319894]
EXISTENCE:mutant phenotype:GO:0007059 chromosome segregation [PMID:12386167]
Nonessential serine/threonine protein kinase; possible role in DNA damage response; influences tolerance to high levels of ethanol
EXISTENCE:direct assay:GO:0004674 protein serine/threonine kinase activity [PMID:11404483|PMID:23579499]
EXISTENCE:direct assay:GO:0005816 spindle pole body [PMID:10662594|PMID:12832486]
EXISTENCE:direct assay:GO:0005935 cellular bud neck [PMID:12832486]
EXISTENCE:genetic interaction:GO:1903024 positive regulation of ascospore-type prospore membrane formation [PMID:32788308]
EXISTENCE:mutant phenotype:GO:0000281 mitotic cytokinesis [PMID:11267871]
EXISTENCE:mutant phenotype:GO:0004674 protein serine/threonine kinase activity [PMID:23579499]
EXISTENCE:mutant phenotype:GO:0007096 regulation of exit from mitosis [PMID:11267871]
EXISTENCE:mutant phenotype:GO:0051229 meiotic spindle disassembly [PMID:17660551|PMID:32788308]
EXISTENCE:mutant phenotype:GO:1903024 positive regulation of ascospore-type prospore membrane formation [PMID:32788308]
EXISTENCE:mutant phenotype:GO:1904750 negative regulation of protein localization to nucleolus [PMID:32788308]
Hippo-like kinase of the Mitotic Exit Network; promotes exit by activating the Dbf2p kinase; component of a non-canonical Hippo pathway with Sps1p required for prospore membrane closure, spindle disassembly and sustained release of Cdc14p during meiotic anaphase II; complexes with Sps1p and contributes to its phosphorylation; phosphorylates the RNAPII CTD during mitosis; localizes to the bud neck and SPB during anaphase and telophase; relocalizes to the cytoplasm upon DNA replication stress
ARS110; Autonomously Replicating Sequence; originally referred to as ADE1 ARS
Member of the seripauperin multigene family; active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme
Putative integral membrane protein; member of DUP240 gene family
Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE analysis; can mutate to suppress amber nonsense mutations
EXISTENCE:direct assay:GO:0030371 translation repressor activity [PMID:27609601]
EXISTENCE:direct assay:GO:0043022 ribosome binding [PMID:27609601]
EXISTENCE:direct assay:GO:0043555 regulation of translation in response to stress [PMID:27609601]
Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE analysis
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:26928762]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:11056382]
Putative integral membrane hypothetical protein; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family
EXISTENCE:direct assay:GO:0000329 fungal-type vacuole membrane [PMID:19001347|PMID:26928762]
Putative integral membrane protein; member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
EXISTENCE:mutant phenotype:GO:0005737 cytoplasm [PMID:12101299]
Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
pheromone-regulated DUP240 family protein PRM9
EXISTENCE:direct assay:GO:0005886 plasma membrane [PMID:12101299]
EXISTENCE:physical interaction:GO:0005783 endoplasmic reticulum [PMID:12925749]
Pheromone-regulated protein; contains 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family; PRM9 has a paralog, PRM8, that arose from a segmental duplication
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:26928762|PMID:12925749]
EXISTENCE:direct assay:GO:0005794 Golgi apparatus [PMID:12925749]
EXISTENCE:direct assay:GO:0016020 membrane [PMID:12925749]
EXISTENCE:genetic interaction:GO:0016050 vesicle organization [PMID:12925749]
EXISTENCE:mutant phenotype:GO:0005783 endoplasmic reticulum [PMID:12101299]
EXISTENCE:mutant phenotype:GO:0005886 plasma membrane [PMID:12101299]
EXISTENCE:physical interaction:GO:0016050 vesicle organization [PMID:12925749]
Putative integral membrane protein, involved in vesicle formation; forms complex with Mst27p; member of DUP240 gene family; binds COPI and COPII vesicles; MST28 has a paralog, MST27, that arose from a segmental duplication
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:18427809|PMID:11914276|PMID:14576278|PMID:16823961]
EXISTENCE:mutant phenotype:GO:0009437 carnitine metabolic process [PMID:11329169]
Outer mitochondrial carnitine acetyltransferase; minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated
EXISTENCE:direct assay:GO:0005739 mitochondrion [PMID:32034123]
hypothetical protein; emerging ORF that arose de novo from non-genic locus; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; localizes to mitochondria
oxysterol-binding protein related protein SWH1
EXISTENCE:direct assay:GO:0000138 Golgi trans cisterna [PMID:11408574]
EXISTENCE:direct assay:GO:0005635 nuclear envelope [PMID:12727870]
EXISTENCE:direct assay:GO:0005769 early endosome [PMID:11408574]
EXISTENCE:direct assay:GO:0005783 endoplasmic reticulum [PMID:12727870]
EXISTENCE:direct assay:GO:0008289 lipid binding [PMID:20008566]
EXISTENCE:direct assay:GO:0015918 sterol transport [PMID:20008566]
EXISTENCE:direct assay:GO:0071561 nucleus-vacuole junction [PMID:25420878]
EXISTENCE:direct assay:GO:0120015 sterol transfer activity [PMID:20008566]
EXISTENCE:genetic interaction:GO:0006887 exocytosis [PMID:17004323]
EXISTENCE:genetic interaction:GO:0006897 endocytosis [PMID:15173322]
EXISTENCE:genetic interaction:GO:0015918 sterol transport [PMID:16585271]
EXISTENCE:genetic interaction:GO:0030011 maintenance of cell polarity [PMID:17004323]
EXISTENCE:genetic interaction:GO:0034727 piecemeal microautophagy of the nucleus [PMID:15367582]
Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats and FFAT motif; interacts with ER anchor Scs2p at the nucleus-vacuole junction; regulated by sterol binding; SWH1 has a paralog, OSH2, that arose from the whole genome duplication
EXISTENCE:direct assay:GO:0009277 fungal-type cell wall [PMID:9483793|PMID:11956747]
EXISTENCE:mutant phenotype:GO:0005537 mannose binding [PMID:9851992]
Lectin-like protein involved in flocculation; cell wall protein that binds mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; important for co-flocculation with other yeasts, mediating interaction with specific species; FLO1 has a paralog, FLO5, that arose from a segmental duplication
ARS111; Autonomously Replicating Sequence
Pseudogenic fragment with similarity to flocculins; YAR061W has a paralog, YHR212W-A, that arose from a segmental duplication
Pseudogenic fragment with similarity to flocculins; YAR064W has a paralog, YHR213W-B, that arose from a segmental duplication
Putative GPI protein; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; YAR066W has a paralog, YHR214W, that arose from a segmental duplication
Fungal-specific hypothetical protein; induced in respiratory-deficient cells; YAR068W has a paralog, YHR214W-A, that arose from a segmental duplication
ARS112; Autonomously Replicating Sequence
EXISTENCE:direct assay:GO:0003993 acid phosphatase activity [PMID:8817921]
EXISTENCE:direct assay:GO:0005576 extracellular region [PMID:8817921]
One of three repressible acid phosphatases; glycoprotein that is transported to the cell surface by the secretory pathway; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2; PHO11 has a paralog, PHO12, that arose from a segmental duplication
TEL01R; Telomeric region on the right arm of Chromosome I; composed of an X element core sequence and a short terminal stretch of telomeric repeats