Phaeodactylum tricornutum CCAP 1055/1 chromosome 31, whole genome shotgun sequence.
Eukaryota; Stramenopiles; Bacillariophyta; Bacillariophyceae; Bacillariophycidae; Naviculales; Phaeodactylaceae; Phaeodactylum.
The Phaeodactylum genome reveals the evolutionary history of diatom genomes
Submitted (15-JUN-2017) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (12-AUG-2008) US DOE Joint Genome Institute, 2800 Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA
PROVISIONAL REFSEQ: This record has not yet been subject to final\nNCBI review. The reference sequence is identical to CM000633.\nURL -- http://www.jgi.doe.gov/phaeodactylum\nJGI Project ID: 3634397\nContacts: Chris Bowler (cbowler@biologie.ens.fr)\n Igor Grigoriev (ivgrigoriev@lbl.gov)\nThe clone of P. tricornutum that was sequenced is CCAP1055/1 and is\navailable from the Culture collection of Algae and Protozoa\n(http://www.ccap.ac.uk). This clone represents a monoclonal culture\nderived from a fusiform cell in May 2003 from strain CCCP632, which\nwas originally isolated in 1956 off Blackpool (U.K.). It has been\nmaintained in culture continuously in F/2 medium.\nAnnotation was done by the JGI Annotation team and the Diatom\nConsortium.\nChromosomes 3 and 11 are complete and present in GenBank records\nCP001142 and CP001141, respectively.\nThe JGI and collaborators endorse the principles for the\ndistribution and use of large scale sequencing data adopted by the\nlarger genome sequencing community and urge users of this data to\nfollow them. It is our intention to publish the work of this\nproject in a timely fashion and we welcome collaborative\ninteraction on the project and analysis.\n(http://www.genome.gov/page.cfm?pageID=10506376)\nAnnotated scaffolds were added in December 2008.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: INSDC submitter\nAnnotation Status :: Full annotation\nAnnotation Pipeline :: Eukaryotic Annotation Propagation Pipeline\n##Genome-Annotation-Data-END##
Has EST support. present: 1 || present: 1
Plastidic enolase (phosphopyruvate hydratase), possesses N-terminal bipartite plastid targeting sequence
translocator of the inner chloroplast envelope membrane 110k
putative 110 kD chloroplast inner envelope protein Tic 110 (IEP110), component of protein translocator of inner chloroplast membrane, contains putative bipartite presequence
heat shock protein, N terminus contains putative ER signal peptide (SignalP score = 1.000); putatively chloroplast targeted
Precise function unknown. A member of a family of related epimerases and dehydratases using bound NAD+ as co-factor. Based on sequence similarity, potentially carries out either of two reactions: (1) UDP-D-glucuronate = UDP-D-xylose + CO2, or (2) dTDP-D-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O.
CPS III, carbamoyl-phosphate synthase mitochindrial precursor
CPS III is a mitochondrial enzyme that catalyzes the irreversible formation of carbamoyl phosphate from bicarbonate and ammonium or glutamine. This reperesents the first commited step of the urea cycle. CPS III is known to have an absolute requirement for N-acetylglutamate. EST support appears to be consitutive with higher numbers of ESTs for ammonium and urea grown cultures.
mitochondrial transit peptide predicted at N-terminus
contains HECT-domain; putative element in ubiquitination cycle
contains bipartite plastid targeting presequence, signal- and transit-peptides predicted at N-terminus with phenylalanine at signal peptide cleavage site
Acetylornithine aminotransferase catalyzes the following reaction, N2-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate. This gene is very well conserved in cyanobacteria and plants. In the diatom, it is upregulated under conditions of nitrate starvation.
precursor of desaturase omega-3 desaturase
membrane bound, di-iron oxidoreductase, containing three 'histidine box' motifs. Similar to omega-3 desaturases. Probably involved in the biosynthesis of eicosapentaenoic and docasahexaenoic acids.