Erwinia tasmaniensis Et1/99 plasmid pET09, complete sequence.
type: Entry
definition: Erwinia tasmaniensis Et1/99 plasmid pET09, complete sequence.
sequence version: NC_010695.1
sequence date: 2022-12-24
sequence: sequence
division: CON
label: NC_010695.1
type: RefSeq
SIO_000068: refseq
dblink: PRJNA224116
dblink: SAMEA2272215
keyword: RefSeq
keyword: erwinia
source: Erwinia tasmaniensis Et1/99
organism: Erwinia tasmaniensis Et1/99
taxonomy: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Erwinia.
reference: 1
references: 18462403
reference: 2
comment: REFSEQ INFORMATION: The reference sequence is identical to\nCU468128.1.\nThis project was carried out by\n*Max Planck Institute for Molecular Genetics, Berlin, Germany\n (principle investigator);\n*Julius Kuehn Institute for Plant Protection in Fruit Crops and\n Viticulture, Dossenheim, Germany\n-------------- Genome Center\n Center: Max Planck Institute for Molecular Genetics\n Center code: MPIMG\n--------------.\nThe annotation was added by the NCBI Prokaryotic Genome Annotation\nPipeline (PGAP). Information about PGAP can be found here:\nhttps://www.ncbi.nlm.nih.gov/genome/annotation_prok/\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: NCBI RefSeq\nAnnotation Date :: 12/24/2022 02:11:33\nAnnotation Pipeline :: NCBI Prokaryotic Genome\n Annotation Pipeline (PGAP)\nAnnotation Method :: Best-placed reference protein\n set; GeneMarkS-2+\nAnnotation Software revision :: 6.4\nFeatures Annotated :: Gene; CDS; rRNA; tRNA; ncRNA\nGenes (total) :: 3,784\nCDSs (total) :: 3,667\nGenes (coding) :: 3,584\nCDSs (with protein) :: 3,584\nGenes (RNA) :: 117\nrRNAs :: 8, 7, 7 (5S, 16S, 23S)\ncomplete rRNAs :: 8, 7, 7 (5S, 16S, 23S)\ntRNAs :: 81\nncRNAs :: 14\nPseudo Genes (total) :: 83\nCDSs (without protein) :: 83\nPseudo Genes (ambiguous residues) :: 0 of 83\nPseudo Genes (frameshifted) :: 40 of 83\nPseudo Genes (incomplete) :: 52 of 83\nPseudo Genes (internal stop) :: 25 of 83\nPseudo Genes (multiple problems) :: 29 of 83\nCRISPR Arrays :: 2\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.

sequence
subclass of: SO_0000155
sequence length: 9299
topology: circular
so has quality: SO_0000988
location: 1..9299
location: region:1-9299:1
RO_0002162: 465817

NC_010695
type: RefSeq
SIO_000068: refseq

PRJNA224116
type: BioProject
SIO_000068: bioproject

SAMEA2272215
type: BioSample
SIO_000068: biosample

1
title: The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia
author: Kube, M.
author: Migdoll, A.M.
author: Muller, I.
author: Kuhl, H.
author: Beck, A.
author: Reinhardt, R.
author: Geider, K.
journal: Environ Microbiol
volume: 10
issue: 9
pages: 2211-2222
year: 2008
pubmed: 18462403

18462403
type: PubMed
SIO_000068: pubmed

2
title: Direct Submission
author: Kube, M.
author: Beck, A.
author: Geider, K.
author: Reinhardt, R.
journal: Submitted (27-AUG-2007) Max Planck Institute for Molecular Genetics, proScience Ihnestrasse 73, D-14195 Berlin, Germany

position:1:1
position: 1
reference: sequence
type: ForwardStrandPosition
type: ExactPosition

position:9299:1
position: 9299
reference: sequence
type: ForwardStrandPosition
type: ExactPosition

feature:1-9299:1:source
so part of: sequence
organism: Erwinia tasmaniensis Et1/99
mol type: genomic DNA
strain: Et1/99
type material: type strain of Erwinia tasmaniensis
plasmid: pET09

465817
type: Taxonomy
SIO_000068: taxonomy

ETA_RS00005
type: Gene
subclass of: SO_0000704
RO_0002162: 465817
locus tag: ETA_RS00005
old locus tag: ETA_pET090010
so part of: sequence
location: 1..954
location: region:1-954:1
so has part: feature:1-954:1:exon
subclass of: SO_0000010

position:954:1
position: 954
reference: sequence
type: ForwardStrandPosition
type: ExactPosition

urn:…
SIO_000300: 1
SIO_000628: feature:1-954:1:exon

ETA_RS00005
type: Coding Sequence
subclass of: SO_0000316
RO_0002162: 465817
locus tag: ETA_RS00005
old locus tag: ETA_pET090010
inference: COORDINATES: similar to AA sequence:RefSeq:WP_016946646.1
note: Derived by automated computational analysis using gene prediction method: Protein Homology.
codon start: 1
transl table: 11
product: replication initiation protein
translation: MSELVVFKANELAMSRYDLTEHETKLILCCVALLNPTVENPTRADRTVSFTYQQYAEMMGLKADNAYHRLNSATSELMTRTVEIIYPTGDVSKRIFQWVNLAEFNRRTQSLILVFSEDILPYLFQIKRFIKYNLEHVKSFENKYSMRIYEWLLKELTQRKTHKANIEISLKDFKFMLMLDKNYPEFKELNRWVLKAAMKDINSHSNMNLSIEKRGRPADTLIFQVELDKQIDLVSELAKESIPRSTKSAATTPESSLHSGLGKILHDALTAKIQLTSFEAKFLSDMQSKYDLNGSFSWLSSKQQTTLEKILAKYGRL
SIO_010081: ETA_RS00005
subclass of: SO_0000673
so part of: ETA_RS00005
location: 1..954
location: region:1-954:1
so has part: feature:1-954:1:exon

WP_012443151.1
type: Protein
SIO_000068: ncbiprotein

urn:…
SIO_000300: 1
SIO_000628: feature:1-954:1:exon

ETA_RS00010
type: Gene
subclass of: SO_0000704
RO_0002162: 465817
locus tag: ETA_RS00010
old locus tag: ETA_pET090020
so part of: sequence
location: 962..1387
location: region:962-1387:1
so has part: feature:962-1387:1:exon
subclass of: SO_0000010

position:962:1
position: 962
reference: sequence
type: ForwardStrandPosition
type: ExactPosition

position:1387:1
position: 1387
reference: sequence
type: ForwardStrandPosition
type: ExactPosition

urn:…
SIO_000300: 1
SIO_000628: feature:962-1387:1:exon

ETA_RS00010
type: Coding Sequence
subclass of: SO_0000316
RO_0002162: 465817
locus tag: ETA_RS00010
old locus tag: ETA_pET090020
inference: COORDINATES: similar to AA sequence:RefSeq:WP_006816427.1
note: Derived by automated computational analysis using gene prediction method: Protein Homology.
codon start: 1
transl table: 11
product: helix-turn-helix domain-containing protein
translation: MALVSVTEAARLTGKNRATIHRYIKQGKLSQSTDATGSKKIETSELLRVFGAISASPATALQYDAMQHDATVENELPVAAMQQKIAMLEQLLESKDKEIQRQDDHIDSLKKAMLLIESKLPATPESATQPVMKKSWQFWKK
SIO_010081: ETA_RS00010
subclass of: SO_0000673
so part of: ETA_RS00010
location: 962..1387
location: region:962-1387:1
so has part: feature:962-1387:1:exon

WP_012443152.1
type: Protein
SIO_000068: ncbiprotein

urn:…
SIO_000300: 1
SIO_000628: feature:962-1387:1:exon

ETA_RS00015
type: Gene
subclass of: SO_0000704
RO_0002162: 465817
locus tag: ETA_RS00015
old locus tag: ETA_pET090030
so part of: sequence
location: 1674..3665
location: region:1674-3665:1
so has part: feature:1674-3665:1:exon
subclass of: SO_0000010

position:1674:1
position: 1674
reference: sequence
type: ForwardStrandPosition
type: ExactPosition

position:3665:1
position: 3665
reference: sequence
type: ForwardStrandPosition
type: ExactPosition

urn:…
SIO_000300: 1
SIO_000628: feature:1674-3665:1:exon

ETA_RS00015
type: Coding Sequence
subclass of: SO_0000316
RO_0002162: 465817
locus tag: ETA_RS00015
old locus tag: ETA_pET090030
inference: COORDINATES: similar to AA sequence:RefSeq:WP_000379077.1
note: Derived by automated computational analysis using gene prediction method: Protein Homology.
codon start: 1
transl table: 11
product: site-specific DNA-methyltransferase
translation: MQTELIKELNKVLKAFPQFWNGDKLHRSMVSEAISKKQPDLIKALVTNEKIKSVYGTDVDGVLIFDFDKLRSLLKYKEYWNNSFTKYRNKVGLSSEGKYLDYSSDVVLDFPFKDCFLEGGMTKEDCGKNEIYYNENIAADEIDRLFSPKALVKTARYDKNGKSSKITKISVDDNLIIKGNNLIALNLLKLTHKEKVKLIYIDPPYNTGSDGFKYNDKFNHSTWLTFIKNRLESAKELLSDDAFICCHIDDSESHYLKVLMDMVFGADNYINTFYIQVRYANKTLKQDMSFHKQIEQVHIYRKSNKAEPNLNETEKGFEKYTHYIEELKDGKEMVLGGKKTIIFDESSYRVLKKEPSEIGLKEIWATGSILDGNSSGRFFRDFLSDRKKTDGLGVLYKVYGIGDDGFDYRYFTGPKKESATKGKYYQGVPTAQLNNKNAQNTTPIENFYDLASYFGNCRHEGGVELRSGKKPEFLLKTIINHFSNQNDLILDFHLGSATTCAVAHKMGRRYIGIEQMDYIKDICIPRMVNVINGDKTGISKDVGWQGGGGFIYTELMELNAYFVHEIQKAQSTEELEKLFAVMKIEAHLNYQVALENVLCAEYEVDGIFRKVAFSELELHEQKQLLIEILDKNQLYVNASDMDDSDLNISESDKAFTRSFYGME
SIO_010081: ETA_RS00015
subclass of: SO_0000673
so part of: ETA_RS00015
location: 1674..3665
location: region:1674-3665:1
so has part: feature:1674-3665:1:exon

WP_012443153.1
type: Protein
SIO_000068: ncbiprotein

urn:…
SIO_000300: 1
SIO_000628: feature:1674-3665:1:exon

ETA_RS00020
type: Gene
subclass of: SO_0000704
RO_0002162: 465817
locus tag: ETA_RS00020
old locus tag: ETA_pET090040
so part of: sequence
location: 3670..6306
location: region:3670-6306:1
so has part: feature:3670-6306:1:exon
subclass of: SO_0000010

position:3670:1
position: 3670
reference: sequence
type: ForwardStrandPosition
type: ExactPosition

position:6306:1
position: 6306
reference: sequence
type: ForwardStrandPosition
type: ExactPosition

urn:…
SIO_000300: 1
SIO_000628: feature:3670-6306:1:exon

ETA_RS00020
type: Coding Sequence
subclass of: SO_0000316
RO_0002162: 465817
locus tag: ETA_RS00020
old locus tag: ETA_pET090040
inference: COORDINATES: similar to AA sequence:RefSeq:WP_015966093.1
GO function: GO:0005524 - ATP binding [Evidence IEA]; GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 - hydrolase activity [Evidence IEA]
note: Derived by automated computational analysis using gene prediction method: Protein Homology.
codon start: 1
transl table: 11
product: DEAD/DEAH box helicase family protein
translation: MAKTPKSAGSEKLLFNKLKQFDEPGLFHDHYETPGYVQENLKDALRPYQHGALRYLHYTQRKREEALLHYRHLLFHMATGAGKTMVMAGTILYLFKEYGYQNFIFFVHTDAIIQKTRENLLNPQSPKYLFSQELEIDGEKITIAPVETFPSVPERNTIYLKLSTIHKMHDELNSYRENSITYEDLKEIPLVLLGDEAHHFNAGTKAKGKLKTSPENEEQTWESTIENVLALRPDNRLFEYTATIDLANKDIGNKYRDRVVYQYDLKQFMSDGYSKKVMLLEANQNDGDKMLDAVLLSQYRKLVAADNGITGFKPVILFKSNKIAISKVKQEEFSQLIAAMTPESVRRHMANKKVQLSSDTSIWHKVIQRYADSDLVTVIGQIQEDFNDFNLLNVNKSDLLEENPVLLNTLENTDNPVRAVFAVAKVNEGWDVLNLYDIVRISEQASSSKTGTDSEAQLIGRGARYFPFMYDGKRSFTRRFDTSAKDLSVLEQLHYHTINEPAYIKTLHASLEQADIDVHQDGGGTIEHARLKEDFKKSAVYQTGRLYFNEVEEIESSSRNWETYSLETRFEIPYQTAGEESLDNLTGTTTGMTKPEPLILDERFYRKAMQRVSFYALDNLQRFFPKLTGIREFIRSDAYLGKLIITVTVPQSLDFSAVPAKEKLHLLETVLLRLSENVRRNDQKVKGTYRFISQPVKEVIKDYSLHIDPSVVINQKITAAQTIGKKWYVYDNAILNQLEHRLVKTLETFMPKLRARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTRNADNAYWQVFLEPKGDDRLLDDAWKERMLETLNDRERIVIDENEDVRLVGIKFFANSQMDAFVSDMQNRLNDGESLETASISLPM
SIO_010081: ETA_RS00020
subclass of: SO_0000673
so part of: ETA_RS00020
location: 3670..6306
location: region:3670-6306:1
so has part: feature:3670-6306:1:exon

WP_012443154.1
type: Protein
SIO_000068: ncbiprotein

urn:…
SIO_000300: 1
SIO_000628: feature:3670-6306:1:exon

ETA_RS00025
type: Gene
subclass of: SO_0000704
RO_0002162: 465817
locus tag: ETA_RS00025
old locus tag: ETA_pET090050
so part of: sequence
location: complement(6349..6942)
location: region:6349-6942:-1
so has part: feature:6349-6942:-1:exon
subclass of: SO_0000010

position:6349:-1
position: 6349
reference: sequence
type: ReverseStrandPosition
type: ExactPosition

position:6942:-1
position: 6942
reference: sequence
type: ReverseStrandPosition
type: ExactPosition

urn:…
SIO_000300: 1
SIO_000628: feature:6349-6942:-1:exon

ETA_RS00025
type: Coding Sequence
subclass of: SO_0000316
RO_0002162: 465817
locus tag: ETA_RS00025
old locus tag: ETA_pET090050
inference: COORDINATES: similar to AA sequence:RefSeq:WP_010891053.1
note: Derived by automated computational analysis using gene prediction method: Protein Homology.
codon start: 1
transl table: 11
product: mobilization protein MobX
translation: MSDEIKQIAALIGHHQALEKRVNTLTEEFQAASAQLQRQSEILSKVIRELDSASGNMTETVRKSVISAMDQVAQELKQAGIEQQKPAVNALNQVVKTANDSVNAMRREMSRYTWKSAIYLVLTIFFVLACSVTALTWFMNDGYSRIAEMQRMEAVWQKKAPLADISRCDGKPCVKVDTSTTYGDKGDKENTWMIIKK
SIO_010081: ETA_RS00025
subclass of: SO_0000673
so part of: ETA_RS00025
location: complement(6349..6942)
location: region:6349-6942:-1
so has part: feature:6349-6942:-1:exon

WP_012443155.1
type: Protein
SIO_000068: ncbiprotein

urn:…
SIO_000300: 1
SIO_000628: feature:6349-6942:-1:exon

ETA_RS00030
type: Gene
subclass of: SO_0000704
RO_0002162: 465817
gene: mobA
locus tag: ETA_RS00030
old locus tag: ETA_pET090060
so part of: sequence
location: complement(6955..8064)
location: region:6955-8064:-1
so has part: feature:6955-8064:-1:exon
subclass of: SO_0000010

position:6955:-1
position: 6955
reference: sequence
type: ReverseStrandPosition
type: ExactPosition

position:8064:-1
position: 8064
reference: sequence
type: ReverseStrandPosition
type: ExactPosition

urn:…
SIO_000300: 1
SIO_000628: feature:6955-8064:-1:exon

ETA_RS00030
type: Coding Sequence
subclass of: SO_0000316
RO_0002162: 465817
gene: mobA
locus tag: ETA_RS00030
old locus tag: ETA_pET090060
inference: COORDINATES: similar to AA sequence:RefSeq:WP_012443156.1
note: Derived by automated computational analysis using gene prediction method: Protein Homology.
codon start: 1
transl table: 11
product: mobilization protein MobA
translation: MASYHLSVKTGGKGSASPHAAYIAREGKYAREKDSDLEHKESGNMPAWAAHKPAEFWKAADTSERANGCTYREIEVALPHELKPEQRLELVRDFVRQEIGDRHAYQFAVHNPKAAIAGGEQPHAHIMFSERLNDGIHRDPEQYFRRANNKEPERGGAKKVRFGETPTGRKEHLVAQRERWADLQNEYLERYHHADRVDARSLKAQGIDREPERHLGAGQVQRFDTGQLQAILASREAERQAQQSRDERDSVIDVTTSLREALSERDNVSVKPAQEPEPEAAAGITFDFEKEPDKLNALVSDAMKGIQEEIDLQFLVNDAMAEFQEIHQEMERQKERIALAEKQRQQEKERLRIAEQKRQKPDKGWSFSR
SIO_010081: ETA_RS00030
subclass of: SO_0000673
so part of: ETA_RS00030
location: complement(6955..8064)
location: region:6955-8064:-1
so has part: feature:6955-8064:-1:exon

WP_012443156.1
type: Protein
SIO_000068: ncbiprotein

urn:…
SIO_000300: 1
SIO_000628: feature:6955-8064:-1:exon

ETA_RS00035
type: Gene
subclass of: SO_0000704
RO_0002162: 465817
gene: traD
locus tag: ETA_RS00035
old locus tag: ETA_pET090070
so part of: sequence
location: 8227..8694
location: region:8227-8694:1
so has part: feature:8227-8694:1:exon
subclass of: SO_0000010

position:8227:1
position: 8227
reference: sequence
type: ForwardStrandPosition
type: ExactPosition

position:8694:1
position: 8694
reference: sequence
type: ForwardStrandPosition
type: ExactPosition

urn:…
SIO_000300: 1
SIO_000628: feature:8227-8694:1:exon

ETA_RS00035
type: Coding Sequence
subclass of: SO_0000316
RO_0002162: 465817
gene: traD
locus tag: ETA_RS00035
old locus tag: ETA_pET090070
inference: COORDINATES: similar to AA sequence:RefSeq:WP_016517641.1
note: Derived by automated computational analysis using gene prediction method: Protein Homology.
codon start: 1
transl table: 11
product: conjugal transfer protein TraD
translation: MAKSEKHEQWAADRVQYIRGLKSPNEQQRLMVILTDKADKTAQDVKTLSLLIQAEQAAEKAQEARARVMNLIQAEKRAEAKAARKARDNALYQSAGLLIMSGLVDSQTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTV
SIO_010081: ETA_RS00035
subclass of: SO_0000673
so part of: ETA_RS00035
location: 8227..8694
location: region:8227-8694:1
so has part: feature:8227-8694:1:exon

WP_012443157.1
type: Protein
SIO_000068: ncbiprotein

urn:…
SIO_000300: 1
SIO_000628: feature:8227-8694:1:exon