Zymoseptoria tritici IPO323 chromosome 4, whole genome shotgun sequence.
Zymoseptoria tritici IPO323 (Mycosphaerella graminicola IPO323)
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Zymoseptoria.
Finished Genome of the Fungal Wheat Pathogen Mycosphaerella graminicola Reveals Dispensome Structure, Chromosome Plasticity, and Stealth Pathogenesis
Submitted (06-FEB-2020) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (18-MAY-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA
PROVISIONAL REFSEQ: This record has not yet been subject to final\nNCBI review. The reference sequence is identical to CM001199.\nURL -- http://www.jgi.doe.gov/Mgraminicola\nJGI Project ID: 4000758\nThe DNA was provided by G.H.J. Kema (gert.kema@wur.nl)\nThe strain is available from Fungal Biodiversity Center,\nhttp://www.cbs.knaw.nl\nContacts: Stephen B. Goodwin (sgoodwin@purdue.edu)\n Gert H. J. Kema (gert.Kema@wur.nl)\n Igor Grigoriev (ivgrigoriev@lbl.gov)\nAssembly and annotation done by JGI.\n\n##Genome-Assembly-Data-START##\nAssembly Method :: JAZZ v. 1.0\nAssembly Name :: MYCGR v2.0\nGenome Coverage :: 8.9X\nSequencing Technology :: Sanger\n##Genome-Assembly-Data-END##\n\n##Metadata-START##\nOrganism Display Name :: Mycosphaerella graminicola IPO323\nGOLD Stamp ID :: Gi00795\n##Metadata-END##\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: INSDC submitter\nAnnotation Status :: Full annotation\nAnnotation Pipeline :: Eukaryotic Annotation Propagation Pipeline\n##Genome-Annotation-Data-END##
culture from epitype of Zymoseptoria tritici
Predicted protein has no similarity to anyt protein in databases (June 2006).
Shows sequence similarity to sidC of E. nidulans. This protein encodes a involved in the biosynthesis of ferricrocin Fuarinine C and triacetylfusarinine C, siderophores involved in Iron uptake. Note: this gene is in a cluster with a putative sit1 (MFS) gene, a putative ABC transporter, and a putative gene encoding another protein involved in siderophore biosynthesis.
Shows sequence similarity to siderophore biosynthesis proteins of Aspergilli (though I'm not sure what this designation is based upon) and IucB proteins of Bacteria (involved in siderophore biosynthesis). May have acyltransferase activity. Note this gene is linked to a putative sidC (38917) and SIT1 class protein (75487) involved in siderophore biosynthesis and iron uptake.
putative siderophore-dependent iron transporter
Shows sequence similarity to SIT1 and ARN1 of S. cerevisiae and MirC of E. nidulans. These are Major facilitator superfamily transporters which act with siderophores in iron uptake
ABC transporter, ABC-C family, MRP type. TC3.A.1.208.These transporters mainly transport conjugated compounds and can be involved in metal resistance.
MFS1 putative major facilitator superfamily transporter
MFS1 monosacharide transporter, STL1- like plasma membrane sugar and general transporter, high affinity glucose transport, integral of membrane
succinate dehydrogenase flavoprotein subunit
succinate dehydrogenase (ubiquinone); succinic dehydrogenase; complex II; menaquinol: fumarate oxidoreductase; fumarate reductase complex (that is FRD, involved in anaerobic respiration, repressed in aerobic respiration); succinate dehydrogenase complex (i.
Beta-xylosidase, Glycosyl hydrolases family 43 (PF04616)
Beta-Glucosidase (Glycosyl Hydrolase Family 3) (Signal P secreted)
secreted protein. lightly similar to a predicted protein from Magnaporthe grisea.
proteasome core particle subunit alpha 3
Beta-Ig-H3/fasciclin, fasciclin I family protein
Conserved predicted protein. Probably conserved among filamentous fungi (it matches significantly with hypothetical proteins). Mg 425 amino acids predicted protein. It contains ERM (ezrin, radixin and moesin) domain. Unknown function.
. Similar to CAA81941 HSL1 [Saccharomyces cerevisiae] a Ser/thr protein kinase, coupling septin ring assembly to cell cycle progression.
In Saccharomyces this gene expresses a protein involved in export from the Golgi to plasma membrane; involved in chitin biosynthesis through its role in Chs3p localization; interacts with Arf1p, Bch1p, Fmp50p, Bud7p, and Chs6p.
putative FRE ferric reductase-like transmembrane component
Shows sequence similarity to FRE proteins of S. cerevisiae. These proteins are involved in iron uptake. They are ferroxidase(FET)-dependent reductases.
phosphoenolpyruvate kinase; phosphoenol transphosphorylase pyruvate kinase (phosphorylating); fluorokinase; fluorokinase (phosphorylating); pyruvic kinase; pyruvate phosphotransferase
Bromodomain-containing AAA ATPase protein
Alpha-Glucosidase (No Signal P prediction) (Glycosyl Hydrolase Family 31)
unknown function. No reliable hits with protein databases, neither with hypothetical (not significant). Predicted small (200 amino acids) protein; no Trp and His. Rich in Ala, Ser, Thr, Val, Gly. Probable M graminicola specific protein (novel gene).
Histone acetyltransferase, acetylates lysine 14 on histone H3; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes
P450 with unknown function. This model is locate in a three-gene cluster (p450 + HMG-coA red./2-polyprenylphenol hydroxylase/NADH-cytochrome b5 reductase + FadB which is conserved in A fumigatus and S. nodorum. In G. zeae and M grisea only 2 out of 3 genes were found together.
Major facilitator superfamily member with eight transmembrane regions.
putative ferulic acid Esterase/Feruloyl esterase precursor
Ferulic Acid Esterase/Feruloyl Esterase Precursor
p450 potentially involved in the degradation of fatty acids and/or alkanes. Multiple seq alignment followed by NJ analysis clustered this model with gi41079162; gi3395458, gi29469881, gi70984521 (alkane or fatty acids monooxygenases), and the models GW.2.447.1 ESTEXT_GWP_GW1.C_110666, E_GW.6.936.1, ESTEXT_FGENESH2_PG.C_40295 ESTEXT_GWP_GW1.C_90605.
Proteolysis and peptidolysis. Peptidase S10 domain. Serine peptidases belong to MEROPS peptidase family S10. Unknown function but secreted SCPIII occurrence has been predicted in other fungi and other organisms. Predicted not glycosylated.
guanine nucleotide-binding protein subunit beta-like protein
Belong to TEF-1 and TEAD transcription factor family
DNA-directed RNA polymerase II subunit RPB2
no hits with protein databases. Probable Mg specific protein (novel gene). No Trp and His
Beta-Glucosidase (Glycosyl Hydrolase Family 3)
putative 2-dehydropantoate 2-reductase; Ketopantoate reductase PanE/ApbA family
MgPP2AregA, protein phosphatase 2A regulatory alpha sub-unit
protein phosphatase 2A regulatory alpha sub-unit
cystine transporter cystinosin, transmembranal, CTNS related. PQ loop repeat domains found, critical for the localisation of cystinosin to lysosomes. involved in L-cystine export from lysosomes
conserved hypothetical protein, unknown function, COG4222 Superfamily
Putative homolog of Saccharomyces cerevisiae SWD1, a subunit of the COMPASS (Set1C) complex, which methylates Rad6p ubiquitinated histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
DNA-directed RNA polymerase II subunit RPB1
Beta-Glucosidase (Glycosyl Hydrolase Family 1) (Signal P secreted)
SNF2 family DNA-dependent ATPase domain-containing protein
RNA-binding G protein effector of mating response pathway
In Saccharomyces, this gene expresses an essential, predominantly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
cell division control protein 2 serine/threonine protein kinase
cell division control protein 2; serine/threonine protein kinase, CMGC family, CDC2/CDK subfamily
putative FLP FmtA-like protein, betalactamase
FLP FmtA like protein putative, betalactamase related to penicillin-binding proteins PBP, Beta-lactamase catalyses the opening and hydrolysis of the beta-lactam ring of beta-lactam antibiotics such as penicillin and cephalosporin, probably involved in defense mechanisms, similar to SNM1 from yeast.
P450 monooxygenase putatively acting on a terpene compound or moiety. Similar to three P450s that are part of uncharacterized fungal clusters containing terpene biosynthetic genes. Similarity with these p450s was found by multiple seq alignment and NJ cluster analysis.
RIS1 Group of SWI/SNF DNA-dependent ATPases
No significant hits with protein databases. Predicted small (310 amino acids) protein, with no Trp. Probable Mg specific protein (novel gene) with unknown function.
MFS putative major facilitator superfamily transporter
major facilitator superfamily member, carbohidrate and general substrate transport, permease, putative receptor
phosphoribosyl diphosphate synthase isoform 4
ribose-phosphate diphosphokinase; ribose-phosphate pyrophosphokinase; PRPP synthetase; phosphoribosylpyrophosphate synthetase; PPRibP synthetase; PP-ribose P synthetase; 5-phosphoribosyl-1-pyrophosphate synthetase; 5-phosphoribose pyrophosphory
Glucose-methanol-choline oxidoreductase protein with signal peptide
hypothetical MAPK kinase kinase (MAPKKK), related to S. cerevisiae Ste11 that is involved at least in three pathways including pheromone response, pseudohyphal/invasive growth as well as the high osmolarity response pathway.
secreted peptidase S8, subtilisin-related protease
Peptidase_S8. Subtilase family. Serine protease. Subtilisin, kexin, sedolisin. predicted small (374 aa) secreted protein with probable O-glycosylation at Thr59.
Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; homolog of Saccharomyces cerevisiae SMC1
Putative Swi2/Snf2-related ATPase, component of the SWR1 complex; required for the incorporation of Htz1p into chromatin
unknown function. No reliable hits with protein databases, neither with hypothetical (not significant). Probable M graminicola specific protein (novel gene).
Two cupin domains; metal binding sites. Fungi are believed to utilize oxalate in nutrient availability, pathogenesis, and competition.
HMMPfam hit to Peptidase_S28, Serine carboxypeptidase S28, has a predicted signal peptide.
putative major facilitator superfamily transporter
MFS-MDR transporter belonging to the DHA2 subfamily. These type of MFS transporters are implicated in MDR and secretion of fungal secondary metabolites. Note: this gene is in neighbourhood of a putative MRP-type ABC transporter. Shows sequence similarity to SIT1 and ARN1 of S.
Rho GTPases are regulators of signaling pathways that control actin organization and cell polarity processes in all eukaryotic cells.
ribose-phosphate diphosphokinase; ribose-phosphate pyrophosphokinase; PRPP synthetase; phosphoribosylpyrophosphate synthetase; PPRibP synthetase; PP-ribose P synthetase; 5-phosphoribosyl-1-pyrophosphate synthetase; 5-phosphoribose pyrophosphorylase; 5-phosphoribosyl-alpha-1-pyrophosphate synthetase; phosphoribosyl-diphosphate synthetase; phosphoribosylpyrophosphate synthase; pyrophosphoribosylphosphate synthetase; ribophosphate pyrophosphokinase; ribose-5-phosphate pyrophosphokinase
MAM3 putative, DUF21 domain found adjacento to two CBS domain protein, hemolysin-like, required for normal mitochondrial morphology
Vacuolar protein sorting-associated protein Vps5
Vps5 C terminal like. Vacuolar protein sorting-associated protein Vps5
Heme binding domain at C-terminu. Electron tranference. Usually at ER or mitochondria. This displays an unusual secretory signal (for cytb5) at 21/22 aa in the N-terminus. Predicted transmembrane domain at 1-24 aa at N- terminus. Predicted non-cytosolic topolgy.
A putative member of the Polycomb group (Pc-G) of proteins involved in establishing higher order chromatin structure; similar to transcriptional repressor EZH1
pyruvate decarboxylase; alpha-carboxylase; pyruvic decarboxylase; alpha-ketoacid carboxylase
Hypothetical mitogen-activated protein kinase kinase (MAPKK) involved in cell wall integrity signaling pathway
lipid, fatty acid, and isoprenoid metabolism, sphingolipid transport; Patched family; Sterol-sensing 5TM box; Haem peroxidase; vacuolar membrane; transmembrane vacuolar glycoprotein; involved in sphingolipid metabolism
ABC transporter, ABC-G family, WBC type. To this cluster the human breastcancer related protein and the Drosophila eye pigment (white/brown and scarlet) transporters belong. This WBC / EPD cluster is involved in eye pigment precursor transport and in pleiotropic drug resistance.
multiple inositol polyphosphate phosphatase-like protein
MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED
Predicted highly glycosylation. Hits with few proteins, but these hits are not reliable. Similarity with surface proteins is based only in Ser and Thr composition (a commun feature). Probable M graminicola specific protein (novel gene). unknown function.
general negative regulator of transcription subunit 4
In Saccharomyces this gene express a component of the CCR4-NOT transcription regulatory complex, which represses transcription, at least in part, by inhibiting functional TBP-DNA interactions and also aids in transcription elongation; interacts with C-terminal region of Not1p.
secreted oxidoreductase. Probable O-glycosulated at Thr187. Pyridine nucleotide-oxidoreductase; Pyr_redox domain. FAD-containing flavoprotein. Electron transfer.Amino acid transport and metabolism. Energy production and conversion.
glutaminase A, might be related to protein 47167
glutaminase A L-glutamine amidohydrolase
Secreted glucaminase A (usually mitochondrial). Large (804aa) secreted protein. Hypothetically conserved in other fungi. Hydrolase class enzyme that catalyzes the deamination of glutamine to form glutamate and ammonium ions. Theoretical pI: 5.26. Glutaminase A is active only at acid pH.Acid-Base Balance.
galactosyl transferase, sugar transporter family
major facilitator superfamily and sugar transporter superfamily, transmembranal
C-14 sterol reductase, acts in ergosterol biosynthesis
ABFa alfa-L-arabinofuranosidase-A-like protein
alfa-L-arabinofuranosidase-A related protein, related to protein 80849
The putative protein contains SH3-domains and related to yeast Bem1 that is involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24, Ste5, Ste20, and Rsr1.
Small (226 aa) secreted protein. Theoretical pI: 5.01. threonine- glycine-rich protein. Largely glycosyated (predicted) at thr and ser. Predicted conserved fungal protein (hits with hypothetical proteins from fungi). Unknown function.
Protein required for normal intracellular sterol distribution and for sphingolipid metabolism
Thiol-disulfide isomerase. Thioredoxin domain-containing protein. Redox reactions. Reversible oxidation of an active centre disulfide bond. Posttranslational modification, protein turnover, chaperones/ Energy production and conversion. Probable role in protein secretion.
Fungal transcriptional regulatory protein. Aflatoxin biosynthesis regulatory protein. Fungal conserved protein. Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain. GAL4-like domain. Transcription factor activity.
A predicted transmembrane domain (23aa). Large(327 aa) facing the cytoplasm and 22 aa at extracellular space. Predicted largely conserved fungal protein, on the basis of blastp (it matches with hypothetical proteins). Unknown function.
Protein with WD-40 repeats, WD40 domain is found in eukaryotic proteins with wide variety of functions: adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; PWP1 in yeast is involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; putative member of beta-transducin superfamily.
Common central domain of tyrosinase. Copper monooxygenase. Binds two copper ions via two sets of three conserved histidines. This family is related to pfam00372. Melanin biosynthesis from tyrosine. Defense response. Related to human TRP1 and TRP2 (melanogenic enzymes), and plant PPO (polyphenol oxidases).
SMC2 subunit of the condensin complex, which reorganizes chromosomes during cell division; coiled-coil protein of the SMC family involved in chromosome condensation and segregation
Putative transcription factor involved in regulating the osmo-stress response Saccharomyces cerevisiae; member of the MADS-box family of transcription factors.
signal recognition particle receptor beta subunit
MFS-MDR transporter belonging to the DHA2 subfamily. These type of MFS transporters are implicated in MDR and secretion of fungal secondary metabolites.
putative calnexin, calcium-binding protein precursor cnx1, ER protein
SIN3 component; histone deacetylase complex
P450 with unknown function. This model has low similarity with known or predicted p450s. No significant matches with characterized p450s.