Zymoseptoria tritici IPO323 chromosome 5, whole genome shotgun sequence.
Zymoseptoria tritici IPO323 (Mycosphaerella graminicola IPO323)
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Zymoseptoria.
Finished Genome of the Fungal Wheat Pathogen Mycosphaerella graminicola Reveals Dispensome Structure, Chromosome Plasticity, and Stealth Pathogenesis
Submitted (06-FEB-2020) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (18-MAY-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA
PROVISIONAL REFSEQ: This record has not yet been subject to final\nNCBI review. The reference sequence is identical to CM001200.\nURL -- http://www.jgi.doe.gov/Mgraminicola\nJGI Project ID: 4000758\nThe DNA was provided by G.H.J. Kema (gert.kema@wur.nl)\nThe strain is available from Fungal Biodiversity Center,\nhttp://www.cbs.knaw.nl\nContacts: Stephen B. Goodwin (sgoodwin@purdue.edu)\n Gert H. J. Kema (gert.Kema@wur.nl)\n Igor Grigoriev (ivgrigoriev@lbl.gov)\nAssembly and annotation done by JGI.\n\n##Genome-Assembly-Data-START##\nAssembly Method :: JAZZ v. 1.0\nAssembly Name :: MYCGR v2.0\nGenome Coverage :: 8.9X\nSequencing Technology :: Sanger\n##Genome-Assembly-Data-END##\n\n##Metadata-START##\nOrganism Display Name :: Mycosphaerella graminicola IPO323\nGOLD Stamp ID :: Gi00795\n##Metadata-END##\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: INSDC submitter\nAnnotation Status :: Full annotation\nAnnotation Pipeline :: Eukaryotic Annotation Propagation Pipeline\n##Genome-Annotation-Data-END##
culture from epitype of Zymoseptoria tritici
probable oxidoreductase ADHosyase superfam
predicted involved in secondary metabolite biosynthesis
Putative transcription factor related to yeast Mcm1
ZPS1 family protein, secreted, probable cell wall
glutamine phosphoribosylpyrophosphate amidotransferase
GPATase_N, Glutamine amidotransferases class-II (GN-AT)_GPAT- type (GPATase domain)catalyzes the first step in purine biosynthesis. Pribosyltran, (Phosphoribosyl transferase domain). PurF domain, Glutamine phosphoribosylpyrophosphate amidotransferase [Nucleotide transport and metabolism].
Beta-Glucosidase (Glycosyl Hydrolase Family 3)
glutaminase A L-glutamine amidohydrolase
Secreted glucaminase A (usually mitochondrial). Large (710aa) secreted protein. Hypothetically conserved in other fungi. Hydrolase class enzyme that catalyzes the deamination of glutamine to form glutamate and ammonium ions. Theoretical pI: 4.62. Glutaminase A is active only at acid pH.Acid-Base Balance.
putative endo alpha-1,4 polygalactosaminidase precusor
putative polygalactosaminidase reductase, belongs to DUF297 superfamily. Secreted
Non transporter ABC protein, ABC-F family, GCN / EF3 type. EF-3 Elongation factor 3 ABC proteins are cytosolic proteins required by fungal ribosomes for in vitro protein synthesis and for in vivo growth. EF-3 stimulates the binding of the EF-1:GTP:aa-tRNA ternary complex to the ribosomal A site by facilitated release of the deacylated tRNA from the E site.
Contains predicted signal peptide. Shows sequence similarity to N. crassa hypothetical protein.
serine/threonine protein kinase, CMGC family, CDC2/CDK subfamily
secreted small (74aa) peptide. Predicted glycosylated at Ser74. No hits with protein databases, neither hypothetical. Probable Mg specific protein (novel gene).Unknown function.
Phytochrome photoreceptors sense red and far-red light through photointerconversion between two stable conformations, a process mediated by a linear tetrapyrrole chromophore. Originally, phytochromes were thought to be confined to photosynthetic organisms including cyanobacteria, but they have been recently discovered in heterotrophic bacteria and fungi, where little is known about their functions.
phosphoenolpyruvate carboxykinase (ATP); phosphopyruvate carboxylase (ATP); phosphoenolpyruvate carboxylase; phosphoenolpyruvate carboxykinase; phosphopyruvate carboxykinase (adenosine triphosphate); PEP carboxylase; PEP carboxykinase; PEPCK (ATP); PEPK; PEPCK; phosphoenolpyruvic carboxylase; phosphoenolpyruvic carboxykinase; phosphoenolpyruvate carboxylase (ATP); phosphopyruvate carboxykinase
beta 1,3-galactosyltransferase. UDP-galactose:N-acetylgalactosamine-alpha-R beta 1,3-galactosyltransferase. Predicted. small (490aa), theoretical pI: 6.08. Conserved protein (reliable hits with fungal hypothetical proteins). Belongs to small gene family.
aminophospholipid-translocating P4-type ATPase
Nitrogen regulatory protein GLN3, Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source
Ca2+-modulated nonselective cation channel polycystin
related to intracellular calcium signaling
chromatin assembly complex 1 subunit B/CAC2
Similar to Saccharomyces cerevisiae CAC2 protein which is a component of the chromatin assembly complex (with Rlf2p and Msi1p) that assembles newly synthesized histones onto recently replicated DNA
Similar to Saccharomyces cerevisiae SPT5, a protein that forms a complex with Spt4p and mediates both activation and inhibition of transcription elongation
Dual-specificity protein phosphatase related to yeast Msg5 that is required for maintenance of a low level of signaling through the cell wall integrity pathway; regulates and is regulated by Slt2p; also required for adaptive response to pheromone.
Peptidase M28 (Pfam: PF04369) with a predicted signal peptide. Similarity to known glutaminyl-peptide cyclotransferases.
Peptidase M24A, methionine aminopeptidase. Seems to lack a signal peptide.
Non-transporter ABC protein belonging to the SMC type. These proteins contain motifs resembling the Walker A and Walker B' motif, and a motif with homology to the signature sequence of the ATP-binding cassette (ABC) family of ATPases. Eukaryotic SMC5 proteins; SMC proteins are large (approximately 110 to 170 kDa), and each is arranged into five recognizable domains.
fumarate hydratase; fumarase; L-malate hydro-lyase
Hypothetical DNA helicase and DNA-dependent ATPase; in yeast involved in DNA repair, required for proper timing of commitment to meiotic recombination and the transition from Meiosis I to Meiosis II; potential Cdc28p substrate
RCC1 domain and calcium binding domain-containing protein
Zinc-binding sites: 6 conserved cysteines and 2 conserved histidines coordinate 2 zinc ions. EFH domain: EF-hand, calcium binding motif; Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins.
ABC transporter, ABC-B family, MDR (II.2) type. TC3.A.1.201. In the yeasts S. cerevisiae and S. pombe these transporters are involved in secretion of mating pheromone.In other fungi they function as MDR transporters. Shows sequence similarity to AtrH of E. nidulans.
glycosyl hydrolase family 28, seems to lack a signal peptide
actin-related protein 2/3 complex subunit ARC19
secreted small (462aa) protein; predicted highly glycosylated, probable proteoglycan. (Probable specific MG protein (novel gene): No reliable hits with protein databases, neither with hypothetical (not significant). Matches occur only at ser, thr or pro.Theoretical pI: 5.67 Unknown function.
small (69aa) secreted peptide. No hits with protein databases, neither with hypothetical. No Trp, Phe, Val and glu. Probable M graminicola specific protein (novel gene). Unknown function.
P450 with unknown function. It may work in a polyketide pathway. This model has low similarity with characterized p450s. This model is located close to a C-3 sterol dehydrogenase/3-beta-hydroxysteroid dehydrogenase and acetyl-CoA C-acetyltransferase and ERG10 (ergosterol biosynthetic pathway).
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism
Putative Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis; integral plasma membrane
belongs to Alkppc superfamily, probable acid phosphatase
In Saccharomyces this gene expresses a F-box protein, which is component of the SCF ubiquitin-ligase complex, required for Cln1p and Cln2p degradation; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, and morphogenesis.
catalytic subunit of casein kinase 2, a Ser/Thr protein kinase; involved in regulation of circadian clock in Neurospora crassa and azole drug susceptibility in Candida albicans; Casein kinase II is a cyclic-nucleotide-independent protein kinase which phosphorylates a broad spectrum of both cytoplasmic and nuclear substrates
Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p
unknown function, DUF1123 superfamily; hypothetical protein unknown function
Histone deacetylase (RPD3/HDA1 superfamily) similar to Saccharomyces cerevisiae HOS3 protein
Endo-Beta-1,3(4)-glucanase/Mix-Linked Glucanase (Signal P secreted)
Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, involved in the first step in mevalonate biosynthesis
fungal transcriptional regulatory protein
Secreted protein, similar to phosphatidylinositol/phosphatidylglycerol transfer protein.
Conserved protein (Hit with hypothetical fungal proteins). Similar to Rot1 (supresor of TOR2). Probable involved in protein folding.
Membrane (probable ER membrane bound) which probably follows the secretory system. Predicted highly glycosylated. No reliable hits with protein databases, neither with hypothetical (not significant). It just matches at serine aa. Theoretical pI: 4.87 Probable M graminicola specific protein (novel gene).
Alpha-Mannosidase I (Signal p secreted) (Glycosyl Hydroloase Family 38)
Ca2+/calmodulin-dependent protein kinase
leucine aminopeptidase/pepditase M28/metalloprotease
secreted small (387aa) protease; contains the M28 domain. Theoretical pI: 5.50 Probably involved in pathogenesis
Homolog of Saccharomyces cerevisiae FUN30, a SWI/SNF2 family protein, over expression of the protein affects chromosome stability; potential Cdc28p substrate
phosphopentosisomerase; phosphoriboisomerase; ribose phosphate isomerase; 5-phosphoribose isomerase; D-ribose 5-phosphate isomerase; D-ribose-5-phosphate ketol-isomerase
phosphatidylethanolamine-binding-like protein
Theoretical pI: 5.66 It contains Raf Kinase Inhibitor Protein (RKIP) domain; belongs to a highly conserved family of phospholipid-binding proteins with no significant sequence homology to other proteins. A number of biological roles for PEBP have been identified including serine protease inhibition, membrane biogenesis, and Raf-1 kinase inhibition.
HMMPfam hit to Peptidase_S28, Serine carboxypeptidase S28. Has a predicted signal peptide.
Low similarity to enol-lactone hydrolases. This gene could encode for a protein part of the ketoadipate pathway.
UDP-N-acetylglucosamine:dolichyl phosphate N-acetylglucosamine-1-phosphate transferase-like protein
UDP-N-acetylglucosamine:dolichyl phosphate N-acetylglucosamine-1-phosphate transferase belongs to Glycosyl transferase, family 4
carbamoyl-phosphate synthase (glutamine-hydrolysing) large subunit
beta carboxy-cis-cis-muconate lactonizing enzyme, cyloisomerase, CLME1
Enzyme part of the ketoadipate pathway, a degradation route for the utilization of aromatic compounds. Putative beta carboxy-cis-cis-muconate lactonizing enzyme (cyloisomerase).
Putative homolog of Saccharomyces cerevisiae RSC4, one of 15 subunits of the Remodel the Structure of Chromatin (RSC) complex; contains two bromodomains
No reliable hits with protein databases, neither with hypothetical (not significant). Probable M graminicola specific protein (novel gene). Unknown function.
putative major facilitator superfamily transporter
MFS-MDR transporter belonging to the DHA2 subfamily. These type of MFS transporters are implicated in MDR and secretion of fungal secondary metabolites.
Probable sulphate transporter, similar to cys14 of N. crassa. Note there are four sulphate transporter-like proteins in this species.
Beta-Glucosidase (Glycosyl Hydrolase Family 3) (Avenacinase-like)
peroxisomal hydratase-dehydrogenase-epimerase
Ca2+-modulated nonselective cation channel polycystin related to intracellular calcium signal
small mitochondrial peptide, predicted. unknown function. No hits with protein databases, neither with hypothetical . small (90 amino acids) peptide; no Trp and Cys. Probable M graminicola specific protein (novel gene).
ABC-G family, PDR type, TC3.A.1.205. Involved in pleiotropic drug resistance
shows sequence similarity to S. cerevisiae FET3 and FET5 proteins. These proteins are ferroxidases involved in iron uptake.
Similar to the Saccharomyces cerevisiae CDC73 subunit of the Paf1p complex, a large complex that binds to and modulates the activity of RNA polymerase II
Glucan 1,3-Beta-Glucosidase (Exo-Beta-1,3-Glucanase) (Full EXG Conserved Domain) (No Signal p secretion predicition, may not be secreted)
Palmitoyltransferase akr1, ankyrin repeat-containing protein akr1
Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats.
Beta-Galactosidase (Glycoside Hydrolase Family 2)
Peptidase S8 (Pfam PF00082) with signal peptide
hypothetical protein, related to lipase 4 precursor (Candida rugosa)
Glucan 1,4-Alpha-Glucosidase (Glucoamylase/Amloglucosidase) (Glycosyl Hydrolase Family 15)
unknown, probably unique to M graminicola; predicted secreted
Catalase is an ubiquitous enzyme found in both prokaryotes and eukaryotes involved in the protection of cells from the toxic effects of peroxides. It calalyses the conversion of hydrogen peroxide to water and molecular oxygen.
putative cation efflux system protein; CDF superfamily; involved in zinc/cadmium resistance
phosphohexose isomerase; phosphohexomutase; oxoisomerase; hexosephosphate isomerase; phosphosaccharomutase; phosphoglucoisomerase; phosphohexoisomerase; phosphoglucose isomerase; glucose phosphate isomerase; hexose phosphate isomerase; D-glucose-6-phosphate ketol-isomerase; GO:0005829:cytosol GO:0005737:cytoplasm
histone acetyltransferase complex subunit
Putative homolog to Saccharomyces cerevisiae ADA2, a transcriptional adaptor protein that is part of the histone acetyltransferase complex ADA/SAGA complexes
Beta-Glucuronidase (Glycosyl Hydrolase Family 2)
In Sacharomyces, this gene expresses a putative membrane protein of unknown function involved in Mn2+ homeostasis; mutants display actin and general growth defects, heterogeneous cell cycle arrests, and pleiotropic defects in cell cycle progression and organelle distribution.