Zymoseptoria tritici IPO323 chromosome 8, whole genome shotgun sequence.
Zymoseptoria tritici IPO323 (Mycosphaerella graminicola IPO323)
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Zymoseptoria.
Finished Genome of the Fungal Wheat Pathogen Mycosphaerella graminicola Reveals Dispensome Structure, Chromosome Plasticity, and Stealth Pathogenesis
Submitted (06-FEB-2020) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (18-MAY-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA
PROVISIONAL REFSEQ: This record has not yet been subject to final\nNCBI review. The reference sequence is identical to CM001203.\nURL -- http://www.jgi.doe.gov/Mgraminicola\nJGI Project ID: 4000758\nThe DNA was provided by G.H.J. Kema (gert.kema@wur.nl)\nThe strain is available from Fungal Biodiversity Center,\nhttp://www.cbs.knaw.nl\nContacts: Stephen B. Goodwin (sgoodwin@purdue.edu)\n Gert H. J. Kema (gert.Kema@wur.nl)\n Igor Grigoriev (ivgrigoriev@lbl.gov)\nAssembly and annotation done by JGI.\n\n##Genome-Assembly-Data-START##\nAssembly Method :: JAZZ v. 1.0\nAssembly Name :: MYCGR v2.0\nGenome Coverage :: 8.9X\nSequencing Technology :: Sanger\n##Genome-Assembly-Data-END##\n\n##Metadata-START##\nOrganism Display Name :: Mycosphaerella graminicola IPO323\nGOLD Stamp ID :: Gi00795\n##Metadata-END##\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: INSDC submitter\nAnnotation Status :: Full annotation\nAnnotation Pipeline :: Eukaryotic Annotation Propagation Pipeline\n##Genome-Annotation-Data-END##
culture from epitype of Zymoseptoria tritici
putative major facilitator superfamily transporter
MFS-MDR transporter belonging to the DHA2 subfamily. These type of MFS transporters are implicated in MDR and secretion of fungal secondary metabolites.
putative Arabinan endo-1,5-alpha-L-arabinosidase
Arabinan Endo-1,5-Alpha-L-Arabinosidase (Family 43 contains both B-xylosidase and Arabinan Endo-1,5-Alpha-L-Arabinosidase;in NCBI blast, it's more related to the latter; does contain partial XynB Conserved Domain-judgement call) (SignaL P secreted)
P450 putatively acting on a cyclic terpene compound or moiety. p450 similar to GA14-synthase (gibberellin biosynthesis) and other p450s found next to terpene biosynthetic genes in other fungi
Secreted Peptidase_S10. carboxypeptidase C family. Serine carboxipeptodase. Peptidase_S10 domain. predicted small (629 aa) with theoretical pI: 4.51, Predicted not glycosylated.
proteasome core particle subunit alpha 4
Related to glucanase B [Lysobacter enzymogenes]AAT77161.1
Probable catalytic subunit of a mannosylinositol phosphorylceramide synthase
Nuclear.Theoretical pI: 4.41 No reliable hits with protein databases, neither with hypothetical(not significant). Matches occur only at glutamic treches. Probable M graminicola specific protein(novel gene). Specific transcription factor?
putative, sucrose transport protein, integral to membrane
glucose-6-phosphate dehydrogenase; NADP-glucose-6-phosphate dehydrogenase; Zwischenferment; D-glucose 6-phosphate dehydrogenase; glucose 6-phosphate dehydrogenase (NADP); NADP-dependent glucose 6-phosphate dehydrogenase; 6-phosphoglucose dehydrogenase; Entner-Doudoroff enzyme; G6PDH; GPD
SNF2 family DNA-dependent ATPase domain-containing protein
Homolog of Saccharomyces cerevisiae INO80, a Swi2/Snf2-related ATPase that forms a large complex, containing actin and several actin-related proteins, that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro
putative CDP-alcohol phosphatidyltransferase
Putative CDP-alcohol phosphatidyltransferase. Shows some sequence similarity to cardiolipin synthase. Predicted signal peptide. Predicted membrane localization.
CMGC family, glycogen synthase kinase subfamily
putative secreted protein unknown function, probable unique to M graminicola
secreted small (93aa) peptide. Theoretical pI: 6.51 No hits with protein databases (neither hypothetical). Probable Mg specific protein (novel gene). Unknown function.
Endo-Beta-1,3-Glucanase (Laminarinase-like)
serine/threonine protein kinase, CMGC family, CDC2/CDK subfamily
ribokinase; deoxyribokinase; ribokinase (phosphorylating); D-ribokinase
involved in cell division, GTP binding Also has a motif with Peptidase M14, carboxypeptidase A
C-5 sterol desaturase, catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis
Similar to the Saccharomyces cerevisiae CTR9 subunit of the Paf1p complex, a large complex that binds to and modulates the activity of RNA polymerase II
In Saccharomyces this gene expresses a transcription factor that is activated by a MAP kinase signaling cascade, activates genes involved in mating or pseudohyphal/invasive growth pathways; cooperates with Tec1p transcription factor to regulate genes specific for invasive growth
ABC1 homolog with a nuclear or mitochondrial subcellular location. The exact molecular functions of these proteins is not clear, however yeast ABC1 suppresses a cytochrome b mRNA translation defect and is essential for the electron transfer in the bc 1 complex.
Predicted moderately glycosylated at few thr .Theoretical pI: 4.35 No reliable hits with protein databases. No His. Probable Mg specific protein (novel gene). Unknown function.
succinate dehydrogenase cytochrome B subunit
succinate dehydrogenase (ubiquinone); succinic dehydrogenase; complex II; menaquinol: fumarate oxidoreductase; fumarate reductase complex (that is FRD, involved in anaerobic respiration, repressed in aerobic respiration); succinate dehydrogenase complex (i.
Histone H3, core histone required for chromatin assembly and chromosome function
Predicted secreted (by PSORTII) protein. Probable conserved fungal protein. Unknown function (it matches with fungal hypothetical proteins). Theoretical pI: 8.27
Predicted highly glycosylation.Theoretical pI: 8.27 No hits with protein databases. Unknown function.
Hypothetical chitin deacetylase; in yeast this protein together with Cda1p are involved in the biosynthesis of ascospore wall component, chitosan and required for proper rigidity of the ascospore wall
sugar transporter, plasma membrane protein
Esterase_lipase domain. Co-esterase domain. The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine. Nucleophilic attack on the carbonyl carbon atom of the ester bond. Putative conserved fungal protein(it matches significatively with hypothetical fungal proteins).
Positively charged, Theoretical pI: 9.87. No hits with protein databases. Probable Mg specific protein (novel gene). Function unknown.
nucleosome remodeling complex, CAF-I subunit
Similar to Saccharomyces cerevisiae MSI1 protein, a subunit of CAF-I (chromatin assembly factor I); this complex regulates the RAS/cAMP pathway via sequestration of Npr1p kinase; localizes to the nucleus and cytoplasm and is homologous to human retinoblastoma binding proteins RbAp48 and RbAp46
putative potassium transporter, integral to plasma membrane
urease accessory protein, required for the insertionof a nickel ion at the active site; involved in postranscriptional modification, protein turnover and chaperons, plant-like urease activation
Glycoside hydrolase. . Probably involved in post-translational modification of cell wall mannoside-containing proteins. Probably envolved in cell wall biogenesis and struchture. Theoretical pI: 4.69
inositol/phosphatidylinositol phosphatase activity
RNA polymerase II elongator complex, subunit
succinate--CoA ligase (GDP-forming) subunit alpha
succinate-CoA ligase (GDP-forming); succinyl-CoA synthetase (GDP-forming); succinyl coenzyme A synthetase (guanosine diphosphate-forming); succinate thiokinase; succinic thiokinase; succinyl coenzyme A synthetase; succinate-phosphorylating enzyme; P-enzyme; SCS; G-STK; succinyl coenzyme A synthetase (GDP-forming); succinyl CoA synthetase, a mitochondrial enzyme of the TCA cycle
serine/threonine-protein phosphatase 2B catalytic subunit
Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.
hypothetical protein, similar to; gi|156063792|ref|XP_001597818.1| hypothetical protein SS1G_02014 [Sclerotinia sclerotiorum. Predicted extracellular
ABC transporter, ABC-C family, MRP type. TC3.A.1.208.These transporters mainly transport conjugated compounds and can be involved in metal resistance.
Similar to Saccharomyces cerevisiae TUP1, a aeneral repressor of transcription, forms complex with Cyc8p
LYS9, Saccharopine dehydrogenase, lysine biosynthesis pathway
Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, which is the seventh step in lysine biosynthesis pathway
The predicted gene product is an Endonuclease/exonuclease/phosphatase family protein (Pfam PF03372) and contains WD40 repeat
major facilitator superfamily MFS_1 protein
LysM (lysin motif) domain. peptidoglycan-binding LysM-like. cell wall catabolism. Probably involved in pathogenesis. Invoved in infection.
No hits with protein databases, neither with hypothetical. Probable M graminicola specific protein (novel genes). Small (194aa) protein, location uncertain (similar score at P-sort). It contains a putative secretion signal.
Histone 2A, core histone required for chromatin assembly and chromosome function
eukaryotic peptide chain release factor subunit 1
SCF ubiquitin ligase complex subunit HRT1
Ca2+/calmodulin-dependent protein kinase
calcium/calmodulin dependent protein kinase C
Enhancer of Polycomb, an essential histone H4/H2A acetyltransferase complex protein
malate dehydrogenase; malic dehydrogenase; L-malate dehydrogenase; NAD-L-malate dehydrogenase; malic acid dehydrogenase; NAD-dependent malic dehydrogenase; NAD-malate dehydrogenase; NAD-malic dehydrogenase; malate (NAD) dehydrogenase; NAD-dependent malate dehydrogenase; NAD-specific malate dehydrogenase; NAD-linked malate dehydrogenase; MDH; L-malate-NAD+ oxidoreductase
erp like protein; emp24/gp25L/p24 family/GOLD protein. Members of this family are implicated in bringing cargo forward from the ER and binding to coat proteins by their cytoplasmic domains.
Similar to Saccharomyces cerevisiae CAC1, the largest subunit (p90) of the Chromatin Assembly Complex (CAF-I) with Cac2p and Msi1p that assembles newly synthesized histones onto recently replicated DNA
Oligopeptide transporter superfamily family protein (Pfam: PF03169) with 16 predicted tansmembrane regions.
Peptidase M28 (Pfam: PF04389) with predicted signal pepitide
ABC-G family, PDR type, TC3.A.1.205. Potentially involved in pleiotropic drug resistance
protein phosphatase 2A regulatory beta sub-unit 2
phosphoribosylformylglycinamidine synthase
Non transporter ABC protein, ABC-F family, GCN / EF3 type. EF-3 Elongation factor 3 ABC proteins are cytosolic proteins required by fungal ribosomes for in vitro protein synthesis and for in vivo growth. EF-3 stimulates the binding of the EF-1:GTP:aa-tRNA ternary complex to the ribosomal A site by facilitated release of the deacylated tRNA from the E site.
chromatin remodeling complex SWI/SNF component SWI2
Chromatin remodeling protein, puative catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation
ATP-binding cassette glutathione S-conjugate transporter
ABC transporter, ABC-C family, MRP type.. TC3.A.1.208.These transporters mainly transport conjugated compounds and can be involved in metal resistance. Gene located next to another MRP-type ABC transporter and in area with genes implicated in protein translation initiation/ elongation/ degradation and nuclear protein export.
tRNA adenine-N(1)--methyltransferase non-catalytic subunit
tRNA (adenine-N(1)-)-methyltransferase non-catalytic subunit. Similar to Gcd10p of Saccharomyces cerevisiae. Translation initiation factor EIF3-related.
P450 with unknown function and possibly involved in sterol biosynthesis. nidulans AhbB. Mutation at AhbB locus is associated with increased sensitivity to the ergosterol biosynthesis inhibitor imidazole, increased branching and abnormal conidiophore development relative to wild type at restrictive temperature.
serine/threonine protein kinase complex beta subunit
Similar to YGL208W (S. cerevisiae) which is the one of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane.
chromosome condensation complex Condensin, subunit G
Hypothetical related to p21-activated protein kinases (PAKs); PAK members in S. cerevisiae are Cla4, Ste20 and Skm1 that iteract with the effector of Cdc42 to regulate MAP kinase pathways
L-tryptophan catabolism: kynurenine => anthranilate + L-alanine+ H20.
RAD16 class DNA helicase and DNA-dependent ATPase
Acyltransferase 3; COG-1835 containing domain protein. Fatty acid and phospholipid metabolism. Probable location: plasmatic.
. The predicted gene product is similar to Aspergillus nidulans AfeAp, an adenylate-forming enzyme.
Peptidase_S10. carboxypeptidase C family. Serine carboxipeptodase. Peptidase_S10 domain. predicted small (523 aa) secreted protein with probable O-glycosylation at Thr462
A nucleosome assembly-like protein with more similarity to Homo sapiens TSPY, TSPLY and SET translocation (myeloid leukemia-associated)proteins than Saccharomyces cerevisiae NAP1
Ca2+-modulated nonselective cation channel
nonselective cation channel related to an increase in intracellular Ca2+
Deacylase, similar to. Also similar to succinylglutamate desuccinylase / Aspartoacylase. flavoprotein; iron-sulfur protein. It conatins AstE_AspA Domain and Zn_pep domain. Negatively charged.Theoretical pI: 4.28 Secreted (according to PSortII and SignalP) small (372aa) protein.
1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
p450 potentially involved in the degradation of fatty acids and/or alkanes. Multiple seq alignment followed by NJ analysis clustered this model with gi41079162; gi3395458, gi29469881, gi70984521 (alkane or fatty acids monooxygenases), and the models GW.2.447.1 ESTEXT_GWP_GW1.C_110666, ESTEXT_FGENESH2_PG.C_40295, ESTEXT_FGENESH2_PM.C_50060, ESTEXT_GWP_GW1.C_90605.
Conserved fungal protein. Involved in degradation of complex cell wall polysaccharides; pectin and xylan degradation. Involved in degradation of plant cell walls. Hydrolyzes the feruloyl-arabinose ester bond in arabinoxylans, and the feruloyl-galactose ester bond in pectin.
putative fungal pheromone STE3 a-factor receptor
ABC transporter, ABC-G family, WBC type. To this cluster the human breastcancer related protein and the Drosophila eye pigment (white/brown and scarlet) transporters belong. This WBC / EPD cluster is involved in eye pigment precursor transport and in pleiotropic drug resistance.
Serine and threonine rich protein. Predicted highly O-glysolylated. Probable required for hyphae formation, growth, and Invasion. Probable involved in pathogenesis.
alpha-1, 2-mannosyltransferase glycoside hydrolase family 15
alpha-1, 2-mannosyltransferase belongs to Glycosyl transferase, family 15
Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
RNA binding zinc finger protein, RanBP2-type
AMP-extracellular domain-containing protein
AMP-binding enzyme. A predicted transmembrane domain (22aa). Short N-terminus (18aa) cytoplasmic, and 553 aa containing the AMP domain at the extracellular space. Probable secreted after signal peptide cleavage. Predicted largely conserved fungal protein, on the basis of blastp (it matches with hypothetical proteins).
No hits with protein databases, Probable M graminicola specific protein (novel gene). Theoretical pI: 4.30 Predicted largely O-glycosylated. Unknown function.
peptidase M3A and M3B, thimet/oligopeptidase F