Zymoseptoria tritici IPO323 chromosome 12, whole genome shotgun sequence.
Zymoseptoria tritici IPO323 (Mycosphaerella graminicola IPO323)
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Zymoseptoria.
Finished Genome of the Fungal Wheat Pathogen Mycosphaerella graminicola Reveals Dispensome Structure, Chromosome Plasticity, and Stealth Pathogenesis
Submitted (06-FEB-2020) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (18-MAY-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA
PROVISIONAL REFSEQ: This record has not yet been subject to final\nNCBI review. The reference sequence is identical to CM001207.\nURL -- http://www.jgi.doe.gov/Mgraminicola\nJGI Project ID: 4000758\nThe DNA was provided by G.H.J. Kema (gert.kema@wur.nl)\nThe strain is available from Fungal Biodiversity Center,\nhttp://www.cbs.knaw.nl\nContacts: Stephen B. Goodwin (sgoodwin@purdue.edu)\n Gert H. J. Kema (gert.Kema@wur.nl)\n Igor Grigoriev (ivgrigoriev@lbl.gov)\nAssembly and annotation done by JGI.\n\n##Genome-Assembly-Data-START##\nAssembly Method :: JAZZ v. 1.0\nAssembly Name :: MYCGR v2.0\nGenome Coverage :: 8.9X\nSequencing Technology :: Sanger\n##Genome-Assembly-Data-END##\n\n##Metadata-START##\nOrganism Display Name :: Mycosphaerella graminicola IPO323\nGOLD Stamp ID :: Gi00795\n##Metadata-END##\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: INSDC submitter\nAnnotation Status :: Full annotation\nAnnotation Pipeline :: Eukaryotic Annotation Propagation Pipeline\n##Genome-Annotation-Data-END##
culture from epitype of Zymoseptoria tritici
MVD1, mevalonate pyrophosphate decarboxylase
Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol
No Trp. Predicted highly glycosylated at Thr and Ser. No reliable hits with protein databases, neither with hypothetical (not significant). Probable M graminicola specific protein (novel gene). unknown function.
NEDD4 family E3 ubiquitin-protein ligase
putative beta-galactosidase glycoside hydrolase family 35
Beta-Galactosidase (Glycoside Family 35)
Endo-Rhamnogalacturonase (Glycosyl Hydrolase Family 28) (Signal P secreted)
Small (109aa) secreted protein, predicted. No reliable hits with protein databases, neither with hypothetical (not significant). Probable M graminicola specific protein (novel gene). unknown function.
Secreted beta-lactamase class C, putative. Related to ampicillin, cephalosporine and other penicillin binding proteins. Metal-dependent hydrolases of the beta-lactamase superfamily. Involved in defense mechanisms.
Predicted plasmatic integral protein; high homolgy with hypothetical proteins from many different fungi. Putative conserved fungal protein with unknown function
p450 potentially involved in the degradation of fatty acids and/or alkanes. Multiple seq alignment followed by NJ analysis clustered this model with gi41079162; gi3395458, gi29469881, gi70984521 (alkane or fatty acids monoxygenases), and the models GW.2.447.1 ESTEXT_GWP_GW1.C_110666, E_GW.6.936.1, ESTEXT_FGENESH2_PG.C_40295 ESTEXT_FGENESH2_PM.C_50060.
Large (733aa) predicted integral plasmatic membrane. Probable M graminicola specific protein (novel gene). unknown function.
probable Serine carboxypeptidase, peptidase S10 conserved domain found
putative; emp24/gp25L/p24 family/GOLD. Members of this family are implicated in bringing cargo forward from the ER and binding to coat proteins by their cytoplasmic domains.
predicted conserved fungal protein (homology with fungal hypothetical protein with unknown function), putative mitochondrial, probably membrane bound (specific mitochondrial membrane, not defned). 519aa, pI 5.82. Predicted glycosyated at Ser and Thr.
Member of the major facilitator superfamily of transporters (HOL1 protein-related) with 12 predicted transmembrane regions.
metalloprotease/fungalysin metallopeptidase
Secreted metalloprotease (MEP); Fungalysin metallopeptidase (M36); zinc metallopeptidase; Fungalysin (M36.001) can hydrolyse laminins, elastin, collagen and keratin; they have been related to pathogenesis in fungal pathogens for mammals. The keratinolytic metallopeptidase from Microsporum canium has been used as a vaccine.
asparagine synthase (glutamine-hydrolyzing)
P450 monooxygenase with unknown function.
(pfam PF03169 family) with 17 predicted transmembrane regions.
Large (1060aa) secreted protein; probable fungal conserved protein; homology with fungal hypothetical proteins with unknown function.
small (98aa) peptide, predicted cytoskeleton associated (not bound). pI 5.73. No reliable hits with protein databases, neither with hypothetical (not significant). Probable M graminicola specific protein (novel gene). unknown function.
Non-ribosomal peptide synthetase-like protein
Putative non-ribosomal peptide synthethase gene. High hits with bacterial peptide synthetases involved in o.a. pyoverdine biosynthesis.
biotin-[acetyl-CoA-carboxylase] ligase, putative. Biotin:apoprotein ligase. Acidic (pI 4.38), small (282aa) protein containing GATase1(glutamine amidotransferase)- ScBLP_(biotin-apoprotein ligase)like domains. Covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation.
Beta-Glucosidase (Glycosyl Hydrolase Family 3) (Signal P secreted)
putative secreted alpha-1,2-mannosyltransferase
Secreted alpha-1,2-mannosyltransferase. Glycosyltransferase, group 1.Basic protein, pI 8.81. Probably involved in post-translational modification of cell wall mannoside-containing proteins. Probably envolved in cell wall biogenesis and struchture.Members of this family transfer activated sugars to a variety of substrates, including glycogen, Fructose-6-phosphate and lipopolysaccharides.
Secreted small (249aa) protein; pI 6.52. predicted glycosylated on Thr residuos. No reliable hits with protein databases, neither with hypothetical (not significant). Probable M graminicola specific protein (novel gene). unknown function.
hypothetical alpha-1,2-mannosidase (glyco-hydro 92 superfam). Secreted
Related to yeast Sst2 that is a member of the RGS (regulator of G-protein signaling) family with GTPase-activating protein for Gpa1p inyeast and regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway.
Ca2+-modulated nonselective cation channel polycystin
ATP-binding cassette multidrug transporter
ABC-G family, PDR type, TC3 A.1.205. Involved in pleiotropic drug resistance and azole sensitivity
SDR with unknown function. Could be involved in abscisic acid biosynthesis. Very similar to ABA4 which is part of the abscisic acid gene cluster in B. cinerea.
related to calcium intracelular signaling
The light chain 1 polypeptide is a member of the leucine-rich repeat protein family and binds at or near the ATP hydrolytic site within the motor domain of the gamma heavy chain
Probable conserved secreted small (197aa) peptide. Homology with hypothetical peptides from Chaetomium globosum and Neurospora crassa
unknown protein, putative protein kinase, S-TKc superfam
mitochondrial 54S ribosomal protein YmL9
P450 with unknown function. Multiple seq alignment and NJ analysis clustered this model with gi 39973001 (a P450 in a hybrid PKS-PS cluster and close to a lignin degrading enzyme in M. grisea) and ESTEXT_FGENESH2_PG.C_11264
Ca2+/calmodulin-dependent protein kinase
bifunctional chorismate synthase/flavin reductase
Alpha-1,3-Glucanase (Signal P secreted) (Glycosyl hydrolase Family 71)
calcineurin transcriptional regulator CRZ1
Similar to: lipase 3 precursor; Chain B, Cholesterol Esterase. Peroxisomal, predicted
putative dual spec /; MgPTP7, putative dual spec
Belong to Ras superfamily with GTPases activity.
putative dual spec /; MgPTP2, putative dual spec
MgPP2CL-3, protein phosphatase 2C-like protein 3
putative phosphopantothenoylcysteine decarboxylase
with flavoprotein domain. Also shows some sequence similarity to thymidylate synthase-like DNA metabolism protein of S. pombe.
protein involved in cell divison, with GTP binding motif
Beta-Glucosidase (Glycosyl Hydrolase Family 3)