Deinococcus radiophilus strain ATCC 27603 plasmid pRadio2, complete sequence.
Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae; Deinococcus.
Single Nanopore flow cell sequencing of Deinococcus radiophilus hints at prophage related plasmids: An amateur research perspective
Submitted (04-NOV-2021) Research, Binomica Labs, 4301 22nd St 3FL Studio 342, Long Island City, NY 11101, USA
REFSEQ INFORMATION: The reference sequence is identical to\nCP086382.1.\nBacteria and source DNA available from ATCC 27603 - Carolina\nBiological Supply.\nThe annotation was added by the NCBI Prokaryotic Genome Annotation\nPipeline (PGAP). Information about PGAP can be found here:\nhttps://www.ncbi.nlm.nih.gov/genome/annotation_prok/\n\n##Genome-Assembly-Data-START##\nAssembly Date :: 11-SEP-2020\nAssembly Method :: Trycycler v. 0.3.2\nGenome Representation :: Full\nExpected Final Version :: No\nGenome Coverage :: 800.0x\nSequencing Technology :: Oxford Nanopore MinION\n##Genome-Assembly-Data-END##\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: NCBI RefSeq\nAnnotation Date :: 12/06/2022 04:26:03\nAnnotation Pipeline :: NCBI Prokaryotic Genome\n Annotation Pipeline (PGAP)\nAnnotation Method :: Best-placed reference protein\n set; GeneMarkS-2+\nAnnotation Software revision :: 6.3\nFeatures Annotated :: Gene; CDS; rRNA; tRNA; ncRNA\nGenes (total) :: 2,840\nCDSs (total) :: 2,779\nGenes (coding) :: 2,720\nCDSs (with protein) :: 2,720\nGenes (RNA) :: 61\nrRNAs :: 3, 3, 3 (5S, 16S, 23S)\ncomplete rRNAs :: 3, 3, 3 (5S, 16S, 23S)\ntRNAs :: 49\nncRNAs :: 3\nPseudo Genes (total) :: 59\nCDSs (without protein) :: 59\nPseudo Genes (ambiguous residues) :: 0 of 59\nPseudo Genes (frameshifted) :: 28 of 59\nPseudo Genes (incomplete) :: 30 of 59\nPseudo Genes (internal stop) :: 12 of 59\nPseudo Genes (multiple problems) :: 10 of 59\nCRISPR Arrays :: 2\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
COORDINATES: ab initio prediction:GeneMarkS-2+
Derived by automated computational analysis using gene prediction method: GeneMarkS-2+.
type I-C CRISPR-associated protein Cas8c/Csd1
COORDINATES: similar to AA sequence:RefSeq:WP_013178146.1
GO:0005575 - cellular_component [Evidence IEA]
GO:0003674 - molecular_function [Evidence IEA]
GO:0043571 - maintenance of CRISPR repeat elements [Evidence IEA]
Derived by automated computational analysis using gene prediction method: Protein Homology.
type I-C CRISPR-associated protein Cas5c
COORDINATES: protein motif:HMM:TIGR01587.1
GO:0004386 - helicase activity [Evidence IEA]
COORDINATES: protein motif:HMM:NF024676.2
COORDINATES: similar to AA sequence:RefSeq:WP_014695784.1
adenosylcobinamide-GDP ribazoletransferase
COORDINATES: similar to AA sequence:RefSeq:WP_014695785.1
GO:0008939 - nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity [Evidence IEA]
GO:0009236 - cobalamin biosynthetic process [Evidence IEA]
nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
COORDINATES: similar to AA sequence:RefSeq:WP_012695323.1
GO:0043752 - adenosylcobinamide kinase activity [Evidence IEA]; GO:0000166 - nucleotide binding [Evidence IEA]
bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase
COORDINATES: similar to AA sequence:RefSeq:WP_012695322.1
GO:0003824 - catalytic activity [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_009675799.1
COORDINATES: similar to AA sequence:RefSeq:WP_010883990.1
COORDINATES: similar to AA sequence:RefSeq:WP_012695311.1
GO:0016880 - acid-ammonia (or amide) ligase activity [Evidence IEA]
adenosylcobinamide-phosphate synthase CbiB
COORDINATES: similar to AA sequence:RefSeq:WP_010883988.1
GO:0042242 - cobyrinic acid a,c-diamide synthase activity [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_014695406.1
GO:0008817 - corrinoid adenosyltransferase activity [Evidence IEA]
cob(I)yrinic acid a,c-diamide adenosyltransferase
COORDINATES: similar to AA sequence:RefSeq:WP_013623225.1
ABC transporter substrate-binding protein
COORDINATES: nucleotide motif:Rfam:14.4:RF00174
cobalamin riboswitch; Derived by automated computational analysis using gene prediction method: cmsearch.
COORDINATES: protein motif:HMM:NF021637.2
COORDINATES: similar to AA sequence:RefSeq:WP_013555635.1
GO:0016158 - 3-phytase activity [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_014684119.1
GO:0004803 - transposase activity [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_013615904.1
incomplete; partial in the middle of a contig; missing C-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: protein motif:HMM:NF013677.2
GO:0003677 - DNA binding [Evidence IEA]; GO:0004803 - transposase activity [Evidence IEA]
GO:0006313 - transposition, DNA-mediated [Evidence IEA]
COORDINATES: protein motif:HMM:NF033516.0
COORDINATES: similar to AA sequence:RefSeq:WP_019008172.1
COORDINATES: similar to AA sequence:RefSeq:WP_015234867.1
COORDINATES: similar to AA sequence:RefSeq:WP_010973783.1
frameshifted; internal stop; incomplete; partial in the middle of a contig; missing N-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: similar to AA sequence:RefSeq:WP_012693001.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623248.1
COORDINATES: similar to AA sequence:RefSeq:WP_017869487.1
COORDINATES: protein motif:HMM:NF025615.2
COORDINATES: similar to AA sequence:RefSeq:WP_015723339.1
COORDINATES: similar to AA sequence:RefSeq:WP_013557430.1
GO:0008663 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase activity [Evidence IEA]; GO:0016788 - hydrolase activity, acting on ester bonds [Evidence IEA]; GO:0000166 - nucleotide binding [Evidence IEA]
GO:0009166 - nucleotide catabolic process [Evidence IEA]
bifunctional 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase
COORDINATES: similar to AA sequence:RefSeq:WP_010883973.1
COORDINATES: similar to AA sequence:RefSeq:WP_013555468.1
COORDINATES: similar to AA sequence:RefSeq:WP_011529275.1
COORDINATES: similar to AA sequence:RefSeq:WP_013555410.1
heme ABC transporter ATP-binding protein
COORDINATES: similar to AA sequence:RefSeq:WP_018683801.1
COORDINATES: similar to AA sequence:RefSeq:WP_004210873.1
COORDINATES: protein motif:HMM:NF012873.2
COORDINATES: similar to AA sequence:RefSeq:WP_007532357.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623203.1
COORDINATES: similar to AA sequence:RefSeq:WP_019905887.1
GO:0008168 - methyltransferase activity [Evidence IEA]
methyltransferase domain-containing protein
COORDINATES: similar to AA sequence:RefSeq:WP_013623216.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623175.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623174.1
COORDINATES: similar to AA sequence:RefSeq:WP_015717862.1
COORDINATES: protein motif:HMM:NF025030.2
COORDINATES: protein motif:HMM:NF024824.2
COORDINATES: similar to AA sequence:RefSeq:WP_013557638.1
GO:0005524 - ATP binding [Evidence IEA]; GO:0004797 - thymidine kinase activity [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_012435908.1
5-carboxymethyl-2-hydroxymuconate Delta-isomerase
COORDINATES: similar to AA sequence:RefSeq:WP_013623235.1
fumarylacetoacetate hydrolase family protein
COORDINATES: similar to AA sequence:RefSeq:WP_017869505.1
GO:0008687 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase activity [Evidence IEA]
3,4-dihydroxyphenylacetate 2,3-dioxygenase
COORDINATES: similar to AA sequence:RefSeq:WP_019586248.1
4-hydroxyphenylacetate 3-monooxygenase, oxygenase component
COORDINATES: similar to AA sequence:RefSeq:WP_006132741.1
COORDINATES: similar to AA sequence:RefSeq:WP_010889479.1
GO:0018480 - 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase activity [Evidence IEA]
GO:1901023 - 4-hydroxyphenylacetate catabolic process [Evidence IEA]
5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase
COORDINATES: similar to AA sequence:RefSeq:WP_010889476.1
helix-turn-helix domain-containing protein
COORDINATES: similar to AA sequence:RefSeq:WP_013623242.1
GO:0047470 - (1,4)-alpha-D-glucan 1-alpha-D-glucosylmutase activity [Evidence IEA]
GO:0005992 - trehalose biosynthetic process [Evidence IEA]
COORDINATES: protein motif:HMM:NF013143.2
GO:0043190 - ATP-binding cassette (ABC) transporter complex [Evidence IEA]; GO:0005886 - plasma membrane [Evidence IEA]
GO:0042626 - ATPase-coupled transmembrane transporter activity [Evidence IEA]
GO:0055085 - transmembrane transport [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_019635886.1
metal ABC transporter ATP-binding protein
COORDINATES: protein motif:HMM:NF013465.2
GO:0046872 - metal ion binding [Evidence IEA]
zinc ABC transporter substrate-binding protein
COORDINATES: similar to AA sequence:RefSeq:WP_013615619.1
metal-dependent transcriptional regulator
COORDINATES: similar to AA sequence:RefSeq:WP_013623228.1
COORDINATES: similar to AA sequence:RefSeq:WP_014695574.1
GO:0008767 - UDP-galactopyranose mutase activity [Evidence IEA]
GO:0009273 - peptidoglycan-based cell wall biogenesis [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_014695575.1
COORDINATES: similar to AA sequence:RefSeq:WP_011525778.1
COORDINATES: similar to AA sequence:RefSeq:WP_010884069.1
COORDINATES: similar to AA sequence:RefSeq:WP_012457036.1
type II toxin-antitoxin system HicB family antitoxin
COORDINATES: similar to AA sequence:RefSeq:WP_003611383.1
type II toxin-antitoxin system HicA family toxin
COORDINATES: similar to AA sequence:RefSeq:WP_017871683.1
GO:0003677 - DNA binding [Evidence IEA]; GO:0008170 - N-methyltransferase activity [Evidence IEA]
GO:0006306 - DNA methylation [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_011530673.1
COORDINATES: similar to AA sequence:RefSeq:WP_010883981.1
GO:0005524 - ATP binding [Evidence IEA]; GO:0003676 - nucleic acid binding [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_013616032.1
COORDINATES: protein motif:HMM:TIGR00180.1
ParB/RepB/Spo0J family partition protein
COORDINATES: similar to AA sequence:RefSeq:WP_012695105.1
COORDINATES: similar to AA sequence:RefSeq:WP_013176909.1
frameshifted; incomplete; partial in the middle of a contig; missing N-terminus and C-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: similar to AA sequence:RefSeq:WP_076611084.1
frameshifted; internal stop; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: similar to AA sequence:RefSeq:WP_076612299.1
frameshifted; incomplete; partial in the middle of a contig; missing N-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: similar to AA sequence:RefSeq:WP_012695212.1
COORDINATES: protein motif:HMM:NF025181.2
COORDINATES: similar to AA sequence:RefSeq:WP_076612364.1
programmed frameshift; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: similar to AA sequence:RefSeq:WP_012695215.1
COORDINATES: similar to AA sequence:RefSeq:WP_010887181.1
COORDINATES: protein motif:HMM:NF024627.2
right-handed parallel beta-helix repeat-containing protein
COORDINATES: protein motif:HMM:NF012321.2
COORDINATES: protein motif:HMM:NF021551.2
CRISPR system precrRNA processing endoribonuclease RAMP protein Cas6
COORDINATES: similar to AA sequence:RefSeq:WP_076612365.1
COORDINATES: similar to AA sequence:RefSeq:WP_013616020.1
GO:0015074 - DNA integration [Evidence IEA]
integrase core domain-containing protein
COORDINATES: protein motif:HMM:NF021006.2
TIGR02221 family CRISPR-associated protein
COORDINATES: similar to AA sequence:RefSeq:WP_019011751.1
COORDINATES: protein motif:HMM:NF014505.2
type II toxin-antitoxin system PemK/MazF family toxin
COORDINATES: similar to AA sequence:RefSeq:WP_017326612.1
type II toxin-antitoxin system Phd/YefM family antitoxin
COORDINATES: similar to AA sequence:RefSeq:WP_014263461.1
type II toxin-antitoxin system VapC family toxin
COORDINATES: protein motif:HMM:NF024754.2
COORDINATES: protein motif:HMM:NF024642.2
COORDINATES: protein motif:HMM:NF013753.2
COORDINATES: similar to AA sequence:RefSeq:WP_014686912.1
COORDINATES: similar to AA sequence:RefSeq:WP_014686913.1
COORDINATES: nucleotide motif:Rfam:14.4:RF00059
TPP riboswitch; Derived by automated computational analysis using gene prediction method: cmsearch.
COORDINATES: similar to AA sequence:RefSeq:WP_013622940.1
GO:0051536 - iron-sulfur cluster binding [Evidence IEA]
GO:0009228 - thiamine biosynthetic process [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_011531045.1
GO:0004789 - thiamine-phosphate diphosphorylase activity [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_013622938.1
COORDINATES: similar to AA sequence:RefSeq:WP_015234120.1
COORDINATES: similar to AA sequence:RefSeq:WP_013622936.1
COORDINATES: similar to AA sequence:RefSeq:WP_011531041.1
hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
COORDINATES: similar to AA sequence:RefSeq:WP_010889494.1
GO:0046872 - metal ion binding [Evidence IEA]; GO:0016491 - oxidoreductase activity [Evidence IEA]
2Fe-2S iron-sulfur cluster-binding protein
COORDINATES: similar to AA sequence:RefSeq:WP_010889495.1
xanthine dehydrogenase family protein subunit M
COORDINATES: similar to AA sequence:RefSeq:WP_019546401.1
xanthine dehydrogenase family protein molybdopterin-binding subunit
COORDINATES: similar to AA sequence:RefSeq:WP_014809560.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623201.1
CpsD/CapB family tyrosine-protein kinase
COORDINATES: similar to AA sequence:RefSeq:WP_011757587.1
GO:0003978 - UDP-glucose 4-epimerase activity [Evidence IEA]
GO:0006012 - galactose metabolic process [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_013623246.1
metalloregulator ArsR/SmtB family transcription factor
COORDINATES: similar to AA sequence:RefSeq:WP_013614165.1
COORDINATES: similar to AA sequence:RefSeq:WP_019011906.1
COORDINATES: similar to AA sequence:RefSeq:WP_019327584.1
GO:0050380 - undecaprenyl-diphosphatase activity [Evidence IEA]
GO:0016311 - dephosphorylation [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_013615994.1
COORDINATES: similar to AA sequence:RefSeq:WP_013615995.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623245.1
COORDINATES: similar to AA sequence:RefSeq:WP_014682927.1
COORDINATES: similar to AA sequence:RefSeq:WP_014682926.1
COORDINATES: similar to AA sequence:RefSeq:WP_010887037.1
COORDINATES: similar to AA sequence:RefSeq:WP_011530958.1
mechanosensitive ion channel family protein
COORDINATES: similar to AA sequence:RefSeq:WP_013622950.1
GO:0050660 - flavin adenine dinucleotide binding [Evidence IEA]; GO:0016627 - oxidoreductase activity, acting on the CH-CH group of donors [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_017871724.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623084.1
GO:0008757 - S-adenosylmethionine-dependent methyltransferase activity [Evidence IEA]
GO:0009877 - nodulation [Evidence IEA]; GO:0009312 - oligosaccharide biosynthetic process [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_013623085.1
COORDINATES: similar to AA sequence:RefSeq:WP_011525626.1
incomplete; partial in the middle of a contig; missing N-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: protein motif:HMM:NF019303.2
COORDINATES: similar to AA sequence:RefSeq:WP_013616005.1
COORDINATES: protein motif:HMM:NF018378.2
D-glucuronyl C5-epimerase family protein
COORDINATES: similar to AA sequence:RefSeq:WP_019010369.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623209.1
COORDINATES: similar to AA sequence:RefSeq:WP_019008445.1
COORDINATES: similar to AA sequence:RefSeq:WP_019008443.1
COORDINATES: similar to AA sequence:RefSeq:WP_010883970.1
COORDINATES: similar to AA sequence:RefSeq:WP_019585668.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623217.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623227.1
GO:0004813 - alanine-tRNA ligase activity [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA]; GO:0000166 - nucleotide binding [Evidence IEA]
GO:0006419 - alanyl-tRNA aminoacylation [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_019008854.1
COORDINATES: protein motif:HMM:NF036531.2
GO:0004866 - endopeptidase inhibitor activity [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_011530006.1
COORDINATES: similar to AA sequence:RefSeq:WP_013557591.1
COORDINATES: similar to AA sequence:RefSeq:WP_013557929.1
GO:0051539 - 4 iron, 4 sulfur cluster binding [Evidence IEA]
GO:0006777 - Mo-molybdopterin cofactor biosynthetic process [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_013623197.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623196.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623194.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623195.1
COORDINATES: protein motif:HMM:NF012744.2
COORDINATES: nucleotide motif:Rfam:14.4:RF01051
cyclic di-GMP riboswitch class I; Derived by automated computational analysis using gene prediction method: cmsearch.
COORDINATES: similar to AA sequence:RefSeq:WP_013623192.1
COORDINATES: similar to AA sequence:RefSeq:WP_019584671.1
COORDINATES: similar to AA sequence:RefSeq:WP_019008396.1
HpcH/HpaI aldolase/citrate lyase family protein
COORDINATES: similar to AA sequence:RefSeq:WP_010888848.1
tellurite-like stress resistance cysteine protease StiP
COORDINATES: similar to AA sequence:RefSeq:WP_019010669.1
COORDINATES: similar to AA sequence:RefSeq:WP_019008394.1
COORDINATES: similar to AA sequence:RefSeq:WP_010888844.1
phosphoribosyltransferase domain-containing protein
COORDINATES: similar to AA sequence:RefSeq:WP_019008392.1
COORDINATES: protein motif:HMM:NF014404.2
COORDINATES: similar to AA sequence:RefSeq:WP_126936537.1
COORDINATES: similar to AA sequence:RefSeq:WP_019008375.1
COORDINATES: similar to AA sequence:RefSeq:WP_014778566.1
COORDINATES: similar to AA sequence:RefSeq:WP_005315964.1
COORDINATES: similar to AA sequence:RefSeq:WP_010888855.1
COORDINATES: similar to AA sequence:RefSeq:WP_003486570.1
COORDINATES: similar to AA sequence:RefSeq:WP_019010953.1
COORDINATES: similar to AA sequence:RefSeq:WP_014686104.1
COORDINATES: similar to AA sequence:RefSeq:WP_011529337.1
COORDINATES: similar to AA sequence:RefSeq:WP_019586339.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623164.1
COORDINATES: similar to AA sequence:RefSeq:WP_010889253.1
helix-turn-helix transcriptional regulator
COORDINATES: similar to AA sequence:RefSeq:WP_010889252.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623152.1
COORDINATES: similar to AA sequence:RefSeq:WP_014432729.1
COORDINATES: similar to AA sequence:RefSeq:WP_013623154.1
COORDINATES: protein motif:HMM:NF013098.2
COORDINATES: protein motif:HMM:NF013077.2
COORDINATES: protein motif:HMM:TIGR00697.1
GO:1990397 - queuosine salvage [Evidence IEA]
COORDINATES: protein motif:HMM:NF016330.2
GO:0008270 - zinc ion binding [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_013623212.1
GO:0008410 - CoA-transferase activity [Evidence IEA]
COORDINATES: protein motif:HMM:NF012387.2
GO:0003755 - peptidyl-prolyl cis-trans isomerase activity [Evidence IEA]
GO:0000413 - protein peptidyl-prolyl isomerization [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_017870399.1
GO:0004521 - endoribonuclease activity [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_017870398.1
GO:0004519 - endonuclease activity [Evidence IEA]; GO:0046872 - metal ion binding [Evidence IEA]; GO:0003676 - nucleic acid binding [Evidence IEA]
GO:0051607 - defense response to virus [Evidence IEA]; GO:0043571 - maintenance of CRISPR repeat elements [Evidence IEA]
type I-C CRISPR-associated endonuclease Cas1c
COORDINATES: similar to AA sequence:RefSeq:WP_011529384.1
GO:0004518 - nuclease activity [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_011634097.1
type I-C CRISPR-associated protein Cas7/Csd2
COORDINATES: protein motif:HMM:NF021243.2
COORDINATES: similar to AA sequence:RefSeq:WP_012695021.1