Shigella flexneri 2a str. 301 plasmid pCP301, complete sequence.
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Shigella.
Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157
Submitted (19-JUN-2003) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (27-MAY-2001) State Key Laboratory for Moleclular Virology and Genetic Engineering, Microbial Genome Center of Chinese Ministry of Public Health, 6 Rongjing Eastern Street, BDA, Beijing 100176, P.R.China
REVIEWED REFSEQ: This record has been curated by NCBI staff. The\nreference sequence was derived from AF386526.\nRefSeq Category: Reference Genome\n CLI: Clinical Isolate\n FGS: First Genome sequenced\nCOMPLETENESS: full length.
PhoN2 (Apy), periplasmic phosphatase, apyrase, ATP diphosphohydrolase
probably secreted by the Mxi-Spa secretion machinery; function unknown
secreted by the Mxi-Spa secretion machinery
Similar to Shigella flexneri putative reverse transcriptase ref: NP_085197.1 (405 aa). BLAST with identity of 97% in 405 aa. This CDS contains an in-frame stop codon. The sequence has been checked and is believed to be correct.
Similar to Shigella flexneri putative transposase for IS110 ref: NP_085282.1 (398 aa). BLAST with identity of 98% in 398 aa. This CDS contains an in-frame stop codon. The sequence has been checked and is believed to be correct.
Similar to Shigella flexneri orf, conserved hypothetical protein ref: NP_085259.1 (212 aa). BLAST with identity of 99% in 211 aa. This CDS contains an in-frame stop codon. The sequence has been checked and is believed to be correct.
IS2 OrfB; forms an overlapping reading frame with orfA; may form a fusion protein OrfAB due to ribosomal frameshifting; in some cases OrfB is itself expressed; both OrfA and OrfAB bind terminal inverted repeats
IS2 OrfA; forms an overlapping reading frame with orfB; may form a fusion protein OrfAB due to ribosomal frameshifting; both OrfA and OrfAB bind terminal inverted repeats
IpaC, secreted by the Mxi-Spa secretion machinery, required for entry into epithelial cells
IpaB, secreted by the Mxi-Spa secretion machinery, required for entry into epithelial cells
IpgC, cytoplasmic chaperone for IpaB and IpaC
secreted by the Mxi-Spa machinery, function unknown
Mxi-Spa secretion machinery protein MxiH
Mxi-Spa secretion machinery protein MxiI
Mxi-Spa secretion machinery protein MxiJ
Mxi-Spa secretion machinery protein MxiK
Mxi-Spa secretion machinery protein MxiN
Mxi-Spa secretion machinery protein MxiL
Mxi-Spa secretion machinery protein MxiM
Mxi-Spa secretion machinery protein MxiA
Mxi-Spa secretion machinery protein Spa15
Invasion protein InvC; necessary for efficient entry of S.typhimurium into cultured epithelial cells. Probable catalytic subunit of a protein translocase
Mxi-Spa secretion machinery protein Spa13
Mxi-Spa secretion machinery protein Spa32
Mxi-Spa secretion machinery protein Spa33
part of a type III secretory system probably involved in invasion into eukaryotic cells
surface presentation of antigens protein SpaP
Mxi-Spa secretion machinery protein Spa9
Mxi-Spa secretion machinery protein Spa29
Required for surface presentation of invasion plasmid antigens; required for invasion and for secretion of the three ipa proteins
surface presentation of antigens protein SpaS
Similar to Shigella flexneri IS1294 transposase ref: NP_085231.1 (399 aa). BLAST with identity of 98% in 286 aa. This CDS has been truncated. The sequence has been checked and is believed to be correct.
catalyzes the degradation of periplasmic UDP-glucose to uridine, glucose-1-phosphate and inorganic phosphate; specific for uridine nucleotides
bifunctional UDP-sugar hydrolase/5'-nucleotidase
PhoN1, periplasmic non specific acid ohosphatase
controlled cell death protein; post-segregation toxin; toxin addiction system
invasion plasmid antigen, secreted by the Mxi-Spa secretion machinery
Similar to Shigella flexneri orf, conserved hypothetical protein ref: NP_085397.1 (439 aa). BLAST with identity of 77% in 438 aa. This CDS contains an in-frame stop codon. The sequence has been checked and is believed to be correct.
Transfers myristate or laurate, activated on ACP, to the lipid IVA moiety of (KDO)2-(lauroyl)-lipid IVA
lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase
Similar to Shigella flexneri orf, conserved hypothetical protein ref: NP_085323.1 (272 aa). BLAST with identity of 100% in 272 aa. This CDS contains an in-frame stop codon. The sequence has been checked and is believed to be correct.
cleaves origin of transfer and unwinds; oriT relaxase
conjugal transfer pilus acetylation protein TraX
conjugal transfer fertility inhibition protein FinO
outer membrane protease; involved in virulence in many organisms; OmpT; IcsP; SopA; Pla; PgtE; omptin; in Escherichia coli OmpT can degrade antimicrobial peptides; in Yersinia Pla activates plasminogen during infection; in Shigella flexneria SopA cleaves the autotransporter IcsA