Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 chromosome, complete genome.
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales; Campylobacteraceae; Campylobacter.
Re-annotation and re-analysis of the Campylobacter jejuni NCTC11168 genome sequence
The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences
Submitted (12-DEC-2008) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (07-AUG-2006) Submitted on behalf of the Campylobacter sequencing team, Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA. In Collaboration with the Pathogen Molecular Biology Department at The London School of Hygiene & Tropical Medicine, London, Keppel Street, WC1E 7HT. E-mail:ozan.gundogdu@lshtm.ac.uk
Submitted (09-FEB-2000) Submitted on behalf of the Campylobacter sequencing team, Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA E-mail: parkhill@sanger.ac.uk
REVIEWED REFSEQ: This record has been curated by NCBI staff. The\nreference sequence was derived from AL111168.\nRefSeq Category: Reference Genome\n FGS: First Genome sequenced\n MOD: Model Organism\n UPR: UniProt Genome\nNotes:\nDetails of C. jejuni sequencing at the Sanger Centre are available\non the World Wide Web.\n(URL, http://www.sanger.ac.uk/Projects/C_jejuni/).\nCOMPLETENESS: full length.
binds to the dnaA-box as an ATP-bound complex at the origin of replication during the initiation of chromosomal replication; can also affect transcription of multiple genes including itself.
chromosomal replication initiation protein
binds the polymerase to DNA and acts as a sliding clamp
negatively supercoils closed circular double-stranded DNA
DESCRIPTION:Gene expression, mutation analysis[PMID:15632441]
Cj0004 mutants showed wild-type rates of formate-dependent respiration but were unable to respire with sulphite or metabisulphite as electron donors. Periplasmic extracts of wild-type NCTC11168 showed a symmetrical absorption peak (552nm) after addition of sulphite, demonstrating the reduction of cytochrome C. No cytochrome C reduction was observed after addition of sulphite to periplasmic extracts of the Cj0004c mutant
glutamate synthase is composed of subunits alpha and beta; beta subunit is a flavin adenine dinucleotide-NADPH dependent oxidoreductase; provides electrons to the alpha subunit, which binds L-glutamine and 2-oxoglutarate and forms L-glutamate
RNH2; RNase HII; binds manganese; endonuclease which specifically degrades the RNA of RNA-DNA hybrids
EXISTENCE:Gene expression[PMID:15231804]
DESCRIPTION:Gene expression[PMID:15158262]
catalyzes the dehydration of 2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate in valine and isoleucine biosynthesis
DESCRIPTION:Gene expression, mutation analysis[PMID:15632440]
fumarylacetoacetate (FAA) hydrolase family protein
Catalyzes two discrete reactions in the de novo synthesis of purines: the cleavage of adenylosuccinate and succinylaminoimidazole carboxamide ribotide
Catalyzes the rate-limiting step in dNTP synthesis
ribonucleotide-diphosphate reductase subunit alpha
sodium:dicarboxylate family transmembrane symporter
flavin dependent thymidylate synthase; ThyX; thymidylate synthase complementing protein; catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate; the enzyme from Mycobacterium tuberculosis forms homotetramers; uses FAD as a cofactor
CTP synthase; cytidine triphosphate synthetase; catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen; in Escherichia coli this enzyme forms a homotetramer
single-stranded-DNA-specific exonuclease
tRNA Ala anticodon TGC, Cove score 92.14
tRNA Ile anticodon GAT, Cove score 93.24
type IIS restriction/modification enzyme
DESCRIPTION:Gene expression, mutation analysis[PMID:17768253]
flagellar basal body rod modification protein; acts as a scaffold for the assembly of hook proteins onto the flagellar basal body rod
Bacterial signal recognition particle RNA; Updated (2006) note: Running Rfam search revealed new non-coding RNA families within Campylobacter jejuni. Rfam RF00169, Bacterial signal recognition particle RNA was identified within CDS. This SRP is a univerally conserved ribonucleoprotein involved in the co-translational targeting of proteins to membranes
catalyzes a sulfuration reaction to synthesize 2-thiouridine at the U34 position of tRNAs
One of three proteins involved in switching the direction of the flagellar rotation
with FliG and FliN makes up the switch complex which is involved in switching the direction of the flagella rotation
DESCRIPTION::Gene expression, mutation analysis[PMID:17768253]
DESCRIPTION:Gene expression, mutation analysis[PMID:11292815]
DESCRIPTION:Gene expression, mutation analysis[PMID:14985343]
sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; this sigma factor directs late flagellar biosynthesis genes
flagellar biosynthesis RNA polymerase sigma factor
positive regulator of class III flagellar genes
2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase
catalyzes the formation of 3-dehydroshikimate from 3-dehydroquinate in chorismate biosynthesis
DESCRIPTION: similar to AA sequence:INSD:AAC44524.1
DESCRIPTION: similar to AA sequence:INSD:AAF16680.1
DESCRIPTION: similar to AA sequence:INSD:AAF16679.1
DESCRIPTION: similar to AA sequence:INSD:AAF16678.1
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine
catalyzes the formation of fumarate from aspartate
functions in anaerobic transport of C4-dicarboxylate compounds such as fumarate; similar to dcuB
involved in the peptidyltransferase reaction during translation
essential GTPase; exhibits high exchange rate for GTP/GDP; associates with 50S ribosomal subunit; involved in regulation of chromosomal replication
catalyzes the formation of glutamate 5-phosphate from glutamate in proline biosynthesis
modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet) which is important in translation initiation; inactivation of this gene in Escherichia coli severely impairs growth
catalyzes the formation of biotinyl-5'-AMP, also acts as a transcriptional repressor of the biotin operon
biotin--[acetyl-CoA-carboxylase] synthetase
Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit B' is part of the membrane proton channel.
Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit B is part of the membrane proton channel.
Produces ATP from ADP in the presence of a proton gradient across the membrane. The delta subunit is part of the catalytic core of the ATP synthase complex
produces ATP from ADP in the presence of a proton gradient across the membrane; the alpha chain is a catalytic subunit
Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is a regulatory subunit
Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is a regulatory subunit
part of catalytic core of ATP synthase; alpha(3)beta(3)gamma(1)delta(1)epsilon(1); involved in producing ATP from ADP in the presence of the proton motive force across the membrane
MotA/TolQ/ExbB proton channel family protein
forms dimers; may be involved in cell envelope integrity; interacts with outer membrane proteins and with the C-terminal domain of inner membrane protein TolA
FKBP-type peptidyl-prolyl cis-trans isomerase
malonyl CoA-acyl carrier protein transacylase
DESCRIPTION:Gene expression, mutation analysis[PMID:21489995]
catalyzes the hydrolysis of 6-amino-6-deoxyfutalosine
catalyzes the carboxylation of acetyl-CoA to malonyl-CoA; forms a tetramer of AccA2D2 subunits
acetyl-CoA carboxylase carboxyl transferase subunit beta
catalyzes the formation of 4-hydroxyphenylpyruvate from prephenate
zinc-dependent; catalyzes the deacetylation of UDP-(3-O-acyl)-N-acetylglucosamine to UDP-3-O-(3-hydroxytetradecanoyl)-glucosamine in the second step of lipid A biosynthesis
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase
catalyzes the formation of O-phospho-L-homoserine from L-homoserine in threonine biosynthesis from asparate
Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits during initiation of protein synthesis. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex
associates with free 30S ribosomal subunits; essential for efficient processing of 16S rRNA; in Escherichia coli rbfA is induced by cold shock
ABC transporter substrate-binding protein
methyl-accepting chemotaxis signal transduction protein
repeat 1: identical to complement(238817..239964) and 1492913..1493990 (approximate numbers)
TAT pathway signal sequence domain-containing protein
catalyzes the formation of L-aspartate 4-semialdehyde from L-homoserine
23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB
RpmE; there appears to be two types of ribosomal proteins L31 in bacterial genomes; some contain a CxxC motif while others do not; Bacillus subtilis has both types; the proteins in this cluster have the CXXC motif; RpmE is found in exponentially growing Bacilli while YtiA was found after exponential growth; expression of ytiA is controlled by a zinc-specific transcriptional repressor; RpmE contains one zinc ion and a CxxC motif is responsible for this binding; forms an RNP particle along with proteins L5, L18, and L25 and 5S rRNA; found crosslinked to L2 and L25 and EF-G; may be near the peptidyltransferase site of the 50S ribosome
in Escherichia coli RsmE methylates the N3 position of the U1498 base in 16S rRNA; cells lacking this function can grow, but are outcompeted by wild-type; SAM-dependent m(3)U1498 methyltransferase
16S rRNA (uracil(1498)-N(3))-methyltransferase
molybdenum cofactor biosynthesis protein A; together with moaC, is involved in the conversion of a guanosine derivative (GXP) into molybdopterin precursor Z
cyclic pyranopterin monophosphate synthase
UbiA prenyltransferase family catalyzes the transfer of a prenyl group to various acceptors with hydrophobic ring structures in the biosynthesis of respiratory quinones, hemes, chlorophylls, vitamin E, and shikonin
tRNA delta(2)-isopentenylpyrophosphate transferase; IPP transferase; isopentenyltransferase; involved in tRNA modification; in Escherichia coli this enzyme catalyzes the addition of a delta2-isopentenyl group from dimethylallyl diphosphate to the N6-nitrogen of adenosine adjacent to the anticodon of tRNA species that read codons starting with uracil; further tRNA modifications may occur; mutations in miaA result in defects in translation efficiency and fidelity
tRNA Glu anticodon TTC, Cove score 66.15
DESCRIPTION:Gene expression, mutation analysis[PMID:8025686]
iron-uptake ABC transporter ATP-binding protein
iron-uptake ABC transporter substrate-binding protein
EXISTENCE:Gene expression[PMID:15632442]
in Escherichia coli SbmA is involved in uptake of microcin J25; functions along with FhuA, TonB, and ExbB/D in this capacity; in Sinorhizobium meliloti, BacA is essential and required for symbiosis; defects appear to affect the cell envelope
phosphoribosylglycinamide formyltransferase
cleaves off formyl group from N-terminal methionine residues of newly synthesized proteins; binds iron(2+)
hydrolyzes proteins to small peptides; with the ATPase subunits ClpA or ClpX, ClpP degrades specific substrates
ATP-dependent Clp protease proteolytic subunit
DESCRIPTION:Gene expression, protein purification and characterization[PMID:7670637]
Tig; RopA; peptidyl-prolyl cis/trans isomerase; promotes folding of newly synthesized proteins; binds ribosomal 50S subunit; forms a homodimer
involved in the first step of tetrahydrofolate biosynthesis; catalyzes the formation of formate and 2-amino-4-hydroxy-6-(erythro-1,2, 3-trihydroxypropyl)dihydropteridine triphosphate from GTP and water; forms a homopolymer
involved in type III protein export during flagellum assembly
Catalyzes first step of the de novo purine nucleotide biosynthetic pathway
catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate
4-hydroxy-tetrahydrodipicolinate reductase
threonyl-tRNA synthetase; catalyzes the formation of threonyl-tRNA(Thr) from threonine and tRNA(Thr); catalyzes a two-step reaction, first charging a threonine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA
IF-3 has several functions that are required and promote translation initiation including; preventing association of 70S by binding to 30S; monitoring codon-anticodon interactions by promoting disassociation of fMet-tRNA(fMet) from initiation complexes formed on leaderless mRNAs or incorrectly bound noninitiatior tRNAs and complexes with noncanonical start sites; stimulates codon-anticodon interactions at P-site; involved in moving mRNA to the P-site; and in recycling subunits
catalyzes the reduction of N-acetyl-5-glutamyl phosphate to N-acetyl-L-glutamate 5-semialdehyde in arginine biosynthesis and the reduction of N-acetyl-gamma-aminoadipyl-phosphate to N-acetyl-L-aminoadipate-semialdehyde in lysine biosynthesis; involved in both the arginine and lysine biosynthetic pathways; lysine is produced via the AAA pathway, lysine from alpha-aminoadipate
N-acetyl-gamma-glutamyl-phosphate reductase
catalyzes the phosphorylation of N-acetyl-L-glutamate to form N-acetyl-L-glutamate 5-phosphate
catalyzes the methyl esterification of L-isoaspartyl residues that are formed in damaged proteins
protein-L-isoaspartate O-methyltransferase
catalyzes the formation of 5-phospho-alpha-D-ribose 1-diphosphate and nicotinate from nicotinate D-ribonucleotide and diphosphate
B2 or R2 protein; type 1b enzyme; catalyzes the rate-limiting step in dNTP synthesis; converts nucleotides to deoxynucleotides; forms a homodimer and then a multimeric complex with NrdE
ribonucleotide-diphosphate reductase subunit beta
involved in fifth step of pyrimidine biosynthesis; converts orotidine 5'-phosphate and diphosphate to orotate and 5-phospho-alpha-D-ribose 1-diphosphate
Rrf; Frr; ribosome-recycling factor; release factor 4; RF4; recycles ribosomes upon translation termination along with release factor RF-3 and elongation factor EF-G; A GTPase-dependent process results in release of 50S from 70S; inhibited by release factor RF-1; essential for viability; structurally similar to tRNAs
mechanosensitive ion channel family protein
binds directly to 23S ribosomal RNA prior to in vitro assembly of the 50S ribosomal subunit
EXISTENCE:Protein crystalography[PMID:16287129]
molybdenum cofactor biosynthesis protein MoaC; MoaC; along with MoaA is involved in conversion of a guanosine derivative into molybdopterin precursor Z; involved in molybdenum cofactor biosynthesis
cyclic pyranopterin monophosphate synthase accessory protein
catalyzes the formation of N-carbamoyl-L-aspartate from (S)-dihydroorotate in pyrimidine biosynthesis
repeat 1: identical to 147606..148684 and 1492913..1493990 (approximate numbers)
EXISTENCE:Gene expression[PMID:14985343]
DESCRIPTION:Gene expression, mutation analysis[PMID:12107136]
Characterisation work within Campylobacter jejuni shows that Cj0264c is responsible for both trimethylamine-N-oxide (TMAO) and dimethyl sulfoxide (DMSO) reduction
catalyzes the transamination of the branched-chain amino acids to their respective alpha-keto acids
branched-chain amino acid aminotransferase
in Pseudomonas aeruginosa this enzyme is a trimer of dimers; essential for membrane formation; performs third step of type II fatty acid biosynthesis; catalyzes dehydration of (3R)-hydroxyacyl-ACP to trans-2-acyl-ACP
catalyzes the addition of (R)-3-hydroxytetradecanoyl to the glucosamine disaccharide in lipid A biosynthesis
acyl-[acyl-carrier-protein]--UDP-N- acetylglucosamine O-acyltransferase
binds and unfolds substrates as part of the ClpXP protease
ATP-dependent protease ATP-binding subunit ClpX
functions in MreBCD complex in some organisms
in some organisms this protein is a transmembrane protein while in others it is periplasmic; involved in some organisms with other components of the MreBCD complex and with penicillin binding proteins in the periplasm or cell wall
four CarB-CarA dimers form the carbamoyl phosphate synthetase holoenzyme that catalyzes the production of carbamoyl phosphate; CarB is responsible for the amidotransferase activity
carbamoyl phosphate synthase large subunit
Important for the balance of metabolites in the pentose-phosphate pathway
necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites; arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus
catalyzes the formation of lipid A disaccharide from UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate, lipid A disaccharide is a precursor of lipid A that anchors LPS to the OM
catalyzes the conversion of a phosphate monoester to an alcohol and a phosphate
Converts L-aspartate to beta-alanine and provides the major route of beta-alanine production in bacteria. Beta-alanine is essential for the biosynthesis of pantothenate (vitamin B5)
pantoate--beta-alanine ligase; catalyzes the formation of (R)-pantothenate from pantoate and beta-alanine
catalyzes the formation of tetrahydrofolate and 2-dehydropantoate from 5,10-methylenetetrahydrofolate and 3-methyl-2-oxobutanoate
3-methyl-2-oxobutanoate hydroxymethyltransferase
molybdenum ABC transporter ATP-binding protein
molybdenum ABC transporter substrate-binding lipoprotein
catalyzes the formation of S-adenosyl-4-methylthionine-2-oxobutanoate and 7,8-diaminononanoate from S-adenosyl-L-methionine and 8-amino-7-oxononanoate
adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA
the Ctc family of proteins consists of two types, one that contains the N-terminal ribosomal protein L25 domain only which in Escherichia coli binds the 5S rRNA while a subset of proteins contain a C-terminal extension that is involved in the stress response
50S ribosomal protein L25/general stress protein Ctc
Enables the recycling of peptidyl-tRNAs produced at termination of translation
bifunctional chorismate mutase/prephenate dehydratase
catalyzes the formation of L-histidinol phosphate from imidazole-acetol phosphate and glutamate in histidine biosynthesis
the MS-ring anchors the flagellum to the cytoplasmic membrane; part of the flagellar basal body which consists of four rings L, P, S, and M mounted on a central rod
binds to and inhibits the function of flagella specific ATPase FliI
catalyzes the formation of 1-deoxy-D-xylulose 5-phosphate from pyruvate and D-glyceraldehyde 3-phosphate
Catalyzes the carbon methylation reaction in the biosynthesis of ubiquinone
ubiquinone/menaquinone biosynthesis methyltransferase
bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides
catalyzes the formation of 3-phosphonooxypyruvate and glutamate from O-phospho-L-serine and 2-oxoglutarate; required both in major phosphorylated pathway of serine biosynthesis and in the biosynthesis of pyridoxine
3-phosphoserine/phosphohydroxythreonine aminotransferase
FabH; beta-ketoacyl-acyl carrier protein synthase III; catalyzes the condensation of acetyl-CoA with malonyl-ACP to initiate cycles of fatty acid elongation; differs from 3-oxoacyl-(acyl carrier protein) synthase I and II in that it utilizes CoA thioesters as primers rather than acyl-ACPs
involved in acylation of glycerol-3-phosphate to form 1-acyl-glycerol-3 phosphate for use in phospholipid biosynthesis; functions with PlsY
some L32 proteins have zinc finger motifs consisting of CXXC while others do not
catalyzes the formation of nucleoside triphosphate from ATP and nucleoside diphosphate
EXISTENCE:Gene expression[PMID:10515927]
membrane protein responsible for substrate specificity switching from rod/hook-type export to filament-type export
with MotA forms the ion channels that couple flagellar rotation to proton/sodium motive force across the membrane and forms the stator elements of the rotary flagellar machine
With MotB forms the ion channels that couple flagellar rotation to proton/sodium motive force across the membrane and forms the stator elements of the rotary flagellar machine
has 3'-5' exonuclease, 5'-3' exonuclease and 5'-3'polymerase activities, primarily functions to fill gaps during DNA replication and repair
EXISTENCE:Gene expression, mutation analysis[PMID:15731081]
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by uvrB, the uvrA molecules dissociate
N-(5'-phosphoribosyl)anthranilate isomerase
catalyzes the formation of L-tryptophan from L-serine and 1-(indol-3-yl)glycerol 3-phosphate
catalyzes the formation of indole and glyceraldehyde 3-phosphate from indoleglycerol phosphate in tryptophan biosynthesis
This enzyme catalyses the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin in the biosynthetic pathway of tetrahydrofolate
involved in acylation of glycerol-3-phosphate to form 1-acyl-glycerol-3 phosphate for use in phospholipid biosynthesis; functions with PlsX
glycerol-3-phosphate acyltransferase PlsY
catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate
lipoprotein signal peptidase; integral membrane protein that removes signal peptides from prolipoproteins during lipoprotein biosynthesis
catalyzes the oxygen-independent formation of protoporphyrinogen-IX from coproporphyrinogen-III
DESCRIPTION:Gene expression, mutation analysis[PMID:16048946]
EXISTENCE:Gene expression[PMID:15201231]
DESCRIPTION:Gene expression, mutation analysis, protein purification and characterization[PMID:15728904]
DESCRIPTION:Gene expression, mutation analysis[PMID:15201231]
ferredoxin domain-containing integral membrane protein
a small basic protein that is one of the last in the subunit assembly; omission does not prevent assembly but the subunit is inactive; binds central domain of 16S rRNA
Involved in the metabolism of aromatic amino acids
nucleotide binding property based on structural studies of Haemophilus influenzae crystallized protein in PDB Accession Number 1IN0 and NMR studies of Escherichia coli YajQ; the YajQ protein from Pseudomonas synringae appears to play a role in activation of bateriophage phi6 segment L transcription
ferric reductase-like transmembrane protein
in Escherichia coli this periplasmic enzyme was found to encode the periplasmic catalytic subunit of an oxidoreductase; sulfite oxidase activity not demonstrated; requires inner membrane anchor protein YedZ
type 1 subfamily; involved in last step of pyrimidine biosynthesis; converts orotidine 5'-phosphate to UMP and carbon dioxide; OMP decarboxylase; OMPDCase; OMPdecase
Regulates rRNA biosynthesis by transcriptional antitermination
transcription antitermination protein NusB
RibE; 6,7-diimethyl-8-ribityllumazine synthase; DMRL synthase; lumazine synthase; beta subunit of riboflavin synthase; condenses 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate to generate 6,6-dimethyl-8-lumazine (DMRL); riboflavin synthase then uses 2 molecules of DMRL to produce riboflavin (vitamin B12); involved in the last steps of riboflavin biosynthesis; forms a 60mer (icosahedral shell) in both Bacillus subtilis and Escherichia coli; in Bacillus subtilis this 60mer is associated with the riboflavin synthase subunit (alpha) while in Escherichia coli it is not
catalyzes the formation of 2-dehydro-3-deoxy-D-octonate 8-phosphate from phosphoenolpyruvate and D-arabinose 5-phosphate in LPS biosynthesis
2-dehydro-3-deoxyphosphooctonate aldolase
EngA; essential Neisserial GTPase; synchronizes cellular events by interacting with multiple targets with tandem G-domains; overexpression in Escherichia coli suppresses rrmJ mutation; structural analysis of the Thermotoga maritima ortholog shows different nucleotide binding affinities in the two binding domains
catalyzes the formation of shikimate 3-phosphate from shikimate in aromatic amino acid biosynthesis
tryptophanyl-tRNA synthetase; catalyzes a two-step reaction, first charging a tryptophan molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA
seryl-tRNA synthetase; catalyzes a two-step reaction, first charging a serine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA
type III; catalyzes the formation of (R)-4'-phosphopantothenate from (R)-pantothenate in coenzyme A biosynthesis; type III pantothenate kinases are not subject to feedback inhibition from coenzyme A and have a high Km for ATP
allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; some Mycoplasma proteins contain an N-terminal fusion to an unknown domain
glutamyl-tRNA(Gln) amidotransferase subunit C
DESCRIPTION:Gene expression, mutation analysis[PMID:9765558]
DESCRIPTION:Protein purification and characterization[PMID:22665794]
DESCRIPTION:Gene expression[PMID:1732205]
lysyl-tRNA synthetase; class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA molecules for lysine that exist in this organism and in addition can charge the tRNAPyl with lysine in the presence of LysRS1
DESCRIPTION:Gene expression[PMID:2060796]
catalyzes the reaction of glycine with 5,10-methylenetetrahydrofolate to form L-serine and tetrahydrofolate
AroE; catalyzes the conversion of shikimate to 3-dehydroshikimate
transfers the N-acyl diglyceride moiety to the prospective N-terminal cysteine in prolipoprotein
prolipoprotein diacylglyceryl transferase
part of three member fumarate reductase enzyme complex FrdABC which catalyzes the reduction of fumarate to succinate during anaerobic respiration; FrdAB are the catalytic subcomplex consisting of a flavoprotein subunit and an iron-sulfur subunit, respectively; FrdC is the cytochrome b-556 subunit
part of three member fumarate reductase enzyme complex FrdABC which catalyzes the reduction of fumarate to succinate during anaerobic respiration; FrdAB are the catalytic subcomplex consisting of a flavoprotein subunit and an iron-sulfur subunit, respectively; FrdC is the cytochrome b-556 subunit; the catalytic subunits are similar to succinate dehydrogenase SdhAB
EXISTENCE:Gene expression[PMID:16045618]
UDP-N-acetylmuramoylalanine--D-glutamate ligase; involved in peptidoglycan biosynthesis; cytoplasmic; catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine during cell wall formation
UDP-N-acetylmuramoylalanine--D-glutamate ligase
First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan
phospho-N-acetylmuramoyl-pentapeptide- transferase
catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Catalyzes the first of the two reduction steps in the elongation cycle of fatty acid synthesis
succinate dehydrogenase flavoprotein subunit
succinate dehydrogenase iron-sulfur protein
carries the fatty acid chain in fatty acid biosynthesis
FabF; beta-ketoacyl-ACP synthase II, KASII; catalyzes a condensation reaction in fatty acid biosynthesis: addition of an acyl acceptor of two carbons from malonyl-ACP; required for the elongation of short-chain unsaturated acyl-ACP
catalyzes the carboxylation of acetyl-CoA to malonyl-CoA; forms a tetramer composed of two alpha (AccA) and two beta (AccD) subunits; one of the two catalytic subunits that can form the acetyl CoA carboxylase enzyme together with a carrier protein
acetyl-CoA carboxylase carboxyl transferase subunit alpha
catalyzes the interconversion of D-ribulose 5-phosphate to xylulose 5-phosphate
TPP riboswitch (THI element); Updated (2006) note: Running Rfam search revealed new non-coding RNA families within Campylobacter jejuni. Rfam RF00059, TPP riboswitch (THI element) was identified within CDS. Vitamin B(1) in its active form thiamin pyrophosphate (TPP) is an essential coenzyme that is synthesised by coupling of pyrimidine and thiazole moieties in bacteria. The previously detected thiamin-regulatory element, thi box (PMID:12376536) was extended, resulting in a new, highly conserved RNA secondary structure, the THI element. Analysis of operon structures identified a large number of new candidate thiamin-regulated genes, mostly transporters, in various prokaryotic organisms (PMID:1147090)
thiamine biosynthesis protein ThiC; required for the synthesis of the hydromethylpyrimidine moiety of thiamine
catalyzes the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine from N6-(dimethylallyl)adenosine (i(6)A)
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
modifies transcription through interactions with RNA polymerase affecting elongation, readthrough, termination, and antitermination
catalyzes branch migration in Holliday junction intermediates
DESCRIPTION:Gene expression, mutation analysis[PMID:16339953]
DESCRIPTION:Protein purification and characterization, mutation analysis[PMID:17339325]
EXISTENCE:Protein purification and characterization[PMID:16681372]
This family of heme binding proteins are found mainly in bacteria. Characterised within Campylobacter jejuni. Ctb protein is shown to be involved in moderating oxygen flux within Campylobacter jejuni.
DESCRIPTION:Gene expression, mutation analysis[PMID:16045618]
NssR (Nitrosative stress sensing Regulator) controls the expression of a nitrosative stress-responsive regulon in Campylobacter jejuni which includes ctb (Cj0465c) and cgb (Cj1586).
amino acid ABC transporter ATP-binding protein
tRNA Thr anticodon TGT, Cove score 96.83
tRNA Tyr anticodon GTA, Cove score 64.32
tRNA Gly anticodon TCC, Cove score 96.94
tRNA Thr anticodon GGT, Cove score 83.71
EF-Tu; promotes GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis; when the tRNA anticodon matches the mRNA codon, GTP hydrolysis results; the inactive EF-Tu-GDP leaves the ribosome and release of GDP is promoted by elongation factor Ts; many prokaryotes have two copies of the gene encoding EF-Tu
in Escherichia coli BM108, a mutation that results in lack of L33 synthesis had no effect on ribosome synthesis or function; there are paralogous genes in several bacterial genomes, and a CXXC motif for zinc binding and an upstream regulation region of the paralog lacking this motif that are regulated by zinc similar to other ribosomal proteins like L31; the proteins in this group lack the CXXC motif
tRNA Trp anticodon CCA, Cove score 74.26
forms a complex with SecY and SecG; SecYEG forms a protein-conducting channel to which secA binds and translocates targeted polypeptides across the cytoplasmic membrane, a process driven by ATP and a proton-motive force
Modulates Rho-dependent transcription termination
transcription termination/antitermination protein NusG
in Escherichia coli and Methanococcus, this protein autoregulates expression; the binding site in the mRNA mimics the binding site in the 23S rRNA
binds the two ribosomal protein L7/L12 dimers and anchors them to the large ribosomal subunit
present in two forms; L12 is normal, while L7 is aminoacylated at the N-terminal serine; the only multicopy ribosomal protein; 4:1 ratio of L7/L12 per ribosome; two L12 dimers bind L10; critically important for translation efficiency and fidelity; stimulates GTPase activity of translation factors
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates; beta subunit is part of the catalytic core which binds with a sigma factor to produce the holoenzyme
DNA-directed RNA polymerase subunit beta
DNA-directed RNA polymerase subunit beta'
interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone; located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side; mutations in the S12 gene confer streptomycin resistance
binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit
EF-G; promotes GTP-dependent translocation of the ribosome during translation; many organisms have multiple copies of this gene
tRNA Arg anticodon CCT, Cove score 69.48
methyltransferase domain-containing protein
involved in tryptophan biosynthesis; amino acid biosynthesis; converts 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate to C(1)-(3-indolyl)-glycerol 3-phosphate and carbon dioxide and water
rhodanese-like domain-containing protein
protoheme ferro-lyase; catalyzes the insertion of a ferrous ion into protoporphyrin IX to form protoheme; involved in protoheme biosynthesis; in some organisms this protein is membrane-associated while in others it is cytosolic
alanyl-tRNA synthetase; catalyzes a two-step reaction, first charging an alanyl molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA
Maf; overexpression in Bacillus subtilis inhibits septation in the dividing cell
DESCRIPTION: similar to AA sequence:INSD:CAA73776.1
catalyzes the formation of (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate and L-aspartate in purine biosynthesis; SAICAR synthase
phosphoribosylaminoimidazolesuccinocarboxamide synthase
phosphoribosylformylglycinamidine synthase
catalyzes the formation of 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and L-glutamine in purine biosynthesis
phosphoribosylformylglycinamidine synthase I
1-acyl-SN-glycerol-3-phosphate acyltransferase
forms a junction between the M-ring and FlgB during flagella biosynthesis
with FlgF and B makes up the proximal portion of the flagellar basal body rod
with FlgF and C makes up the proximal portion of the flagellar basal body rod; Vibrio parahaemolyticus protein is associated with the polar flagella
aminodeoxychorismate lyase family protein
EXISTENCE:Gene expression[PMID:14702320]
succinyl-CoA synthetase subunit beta; catalyzes the interconversion of succinyl-CoA and succinate
2-oxoglutarate-acceptor oxidoreductase subunit OorD
2-oxoglutarate-acceptor oxidoreductase subunit OorA
2-oxoglutarate-acceptor oxidoreductase subunit OorB
2-oxoglutarate-acceptor oxidoreductase subunit OorC
catalyzes the formation of glutamate-1-semialdehyde from glutamyl-tRNA(Glu) and NADPH; the second step of the pathway is catalyzed by glutamate-1-semialdehyde aminomutase which results in the formation of 5-aminolevulinic acid; functions in porphyrin (tetrapyrroles) biosynthesis; the crystal structure showed a C-terminal dimerization domain that appears to be absent in Chlamydial proteins
prolyl-tRNA synthetase; catalyzes the formation of prolyl-tRNA(Pro) from proline and tRNA(Pro)
transformation of porphobilinogen to hydroxymethylbilane in porphyrin biosynthesis
3-octaprenyl-4-hydroxybenzoate carboxy-lyase
flagellar capping protein; involved in flagellin assembly
Updated (2006) note: Rfam RF00010, Bacterial RNase P class A has now been identified within CDS. Further support given to product function; rnpB, RNA component of RNase P, similar to many e.g. EMBL:BBRNASEP Z30953 B.burgdorferi (B31) RNase P RNA gene, wublastn score; E = 9.3e-14, 69% identity in 204 bp overlap and 74% identity in 71 bp overlap
Involved in peptide bond synthesis; alters the affinity of the ribosome for aminoacyl-tRNA
Catalyzes the phosphorylation of L-glutamate during the proline biosynthesis pathway
pyridine nucleotide-disulfide oxidoreductase
Characterised members of the Multi Antimicrobial Extrusion (MATE) family function as drug/sodium antiporters. These proteins mediate resistance to a wide range of cationic dyes, fluroquinolones,aminoglycosides and other structurally diverse antibodies and drugs. MATE proteins are found in bacteria, archaea and eukaryotes.
tRNA Met anticodon CAT, Cove score 88.22
tRNA Gln anticodon TTG, Cove score 71.34
bifunctional enzyme DHBP synthase/GTP cyclohydrolase II; functions in riboflavin synthesis; converts GTP to 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine; converts ribulose 5-phopshate to 3,4-dihydroxy-2-butanone 4-phosphate
3,4-dihydroxy-2-butanone-4-phosphate synthase
catalyzes the formation of 2-acetolactate from pyruvate, leucine resistant; also known as acetolactate synthase 3 large subunit
acetolactate synthase 3 catalytic subunit
with IlvI catalyzes the formation of 2-acetolactate from pyruvate, the small subunit is required for full activity and valine sensitivity; E.coli produces 3 isoenzymes of acetolactate synthase which differ in specificity to substrates, valine sensitivity and affinity for cofactors; also known as acetolactate synthase 3 small subunit
adds the O-linked and N-linked 3(R)-hydroxy fatty acids to the glucosamine disaccharide during lipid A biosynthesis
UDP-3-O-acylglucosamine N-acyltransferase
Synthesizes oQ from preQ1 in a single S-adenosylmethionine-requiring step
S-adenosylmethionine--tRNA ribosyltransferase-isomerase
This family of proteins are involved in a sec-independent translocation mechanism. They are involved in export of redox proteins with a twin arginine leader motif (S/T-R-R-X-F-L-K). The sec-independent pathway is termed TAT for twin-arginine translocation system. Transport proteins with bound cofactors that require folding prior to export are mainly moved.
Sec-independent protein translocase TatC
mediates the export of protein precursors bearing twin-arginine signal peptides
catalyzes the formation of 4-phospho-L-aspartate from L-aspartate and ATP, in Bacillus, lysine sensitive; regulated by response to starvation.
catalyzes the DNA-template-directed extension of the 3'-end of a DNA strand; the delta' subunit seems to interact with the gamma subunit to transfer the beta subunit on the DNA
DESCRIPTION: similar to AA sequence:INSD:CAB59901.1
this protein catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction; essential for DNA replication and repair of damaged DNA; similar to ligase LigB
catalyzes the formation of FMN from riboflavin and the formation of FAD from FMN; in Bacillus the ribC gene has both flavokinase and FAD synthetase activities
bifunctional riboflavin kinase/FMN adenylyltransferase
major antigenic peptide PEB-cell binding factor
catalyzes the formation of glycerone phosphate and glyceraldehyde 3-phosphate from fructose 1,6, bisphosphate
sodium-dependent transmembrane transport protein
macrolide export ATP-binding protein/permease
DESCRIPTION:Protein purification and characterization[PMID:8809765]
phosphate ABC transporter substrate-binding protein
ATP-binding protein; PstABCS is an ATP dependent phosphate uptake system which is responsible for inorganic phosphate uptake during phosphate starvation
phosphate ABC transporter ATP-binding protein
hydrogenase isoenzymes formation protein
hydrogenase nickel incorporation protein HypA
required for the assembly and function of the DNAX complex which is required for the assembly of the beta subunit onto primed DNA
catalyzes the formation of (R)-2,3-dihydroxy-3-methylbutanoate from (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and isoleucine biosynthesis
DESCRIPTION:Gene expression, mutation analysis[PMID:16194595]
similar to RuvC resolvase with substantial differences; NMR structural information suggests this protein is monomeric; unknown cellular function
Holliday junction resolvase-like protein
this stereospecific enzymes reduces the S isomer of methionine sulfoxide while MsrB reduces the R form; provides protection against oxidative stress
essential enzyme that recycles AMP in active cells; converts ATP and AMP to two molecules of ADP
aspartyl-tRNA synthetase; catalyzes a two-step reaction, first charging an aspartate molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; contains discriminating and non-discriminating subtypes
catalyzes the phosphorylation of NAD to NADP
Era; Escherichia coli Ras-like protein; Bex; Bacillus Era-complementing segment; essential protein in Escherichia coli that is involved in many cellular processes; GTPase; binds the cell membrane through apparent C-terminal domain; mutants are arrested during the cell cycle; Streptococcus pneumoniae Era binds to RNA and Escherichia coli Era binds 16S rRNA and 30S ribosome
heat shock protein involved in degradation of misfolded proteins
ATP-dependent protease ATP-binding subunit HslU
ATP-dependent protease peptidase subunit
in Escherichia coli this protein is wrapped around the base of the L1 stalk
catalyzes the formation of 2-N(omega)-(L-arginino)succinate from L-citrulline and L-aspartate in arginine biosynthesis, AMP-forming
sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; sigma 54 factor is responsible for the expression of enzymes involved in nitrogen assimilation and metabolism; the rhizobia often have 2 copies of this sigma factor; in Rhizobium etli RpoN1 shown to be involved in the assimilation of several nitrogen and carbon sources during free-living aerobic growth and RpoN2 is involved in symbiotic nitrogen fixation; in Bradyrhizobium both RpoN1 and N2 are functional in free-living and symbiotic conditions, rpoN1 gene was regulated in response to oxygen
functions in anaerobic transport of C4-dicarboxylate compounds such as fumarate; similar to DcuA; DcuA and DcuB function as independent and mutually redundant C4-dicarboxylate (aspartate, malate, fumarate and succinate) transporters
One of the components of the high-affinity ATP-driven potassium transport (or KDP) system, which catalyzes the hydrolysis of ATP coupled with the exchange of hydrogen and potassium ions
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. The beta-hairpin of the Uvr-B subunit is inserted between the strands, where it probes for the presence of a lesion
binding of PriA to forked DNA starts the assembly of the primosome, also possesses 3'-5' helicase activity
catalyzes the conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate into 4-hydroxy-3-methyl-2-en-1-yl diphosphate; involved in isoprenoid synthesis
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
part of the flagellar basal body which consists of four rings L,P, S and M mounted on a central rod
EXISTENCE:Gene expression, mutation analysis[PMID:16436427]
AckA utilizes acetate and can acetylate CheY which increases signal strength during flagellar rotation; utilizes magnesium and ATP; also involved in conversion of acetate to aceyl-CoA
GTPase; similar structure to tubulin; forms ring-shaped polymers at the site of cell division; other proteins such as FtsA, ZipA, and ZapA, interact with and regulate FtsZ function
makes up the distal portion of the flagellar basal body rod
5-(carboxyamino)imidazole ribonucleotide mutase
glycyl-tRNA synthetase subunit alpha; GlyRS; class II aminoacyl tRNA synthetase; tetramer of alpha(2)beta(2); catalyzes a two-step reaction; first charging a glycine molecule by linking its carboxyl group to the alpha-phosphate of ATP; second by transfer of the aminoacyl-adenylate to its tRNA
catalyzes the transfer of 2-keto-3-deoxy-D-manno-octulosonic acid to lipid A
3-deoxy-D-manno-octulosonic acid transferase
binds to lower part of 30S body where it stabilizes two domains; required for efficient assembly of 30S; in Escherichia coli this protein has nuclease activity
methylates guanosine-37 in various tRNAs; uses S-adenosyl-L-methionine to transfer methyl group to tRNA
this protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site
phospho-2-dehydro-3-deoxyheptonate aldolase
catalyzes DNA-template-directed extension of the 3'- end of a DNA strand by one nucleotide at a time; main replicative polymerase
forms a trimer; related to eukaryotic protein gephyrin; functions during molybdenum cofactor biosynthesis
molybdenum cofactor biosynthesis protein MogA
type I phosphodiesterase/nucleotide pyrophosphatase
DESCRIPTION: similar to AA sequence:INSD:AAC45420.1
Acts as a negative regulator of the grpE-dnaK-dnaJ and groELS class I heat shock operons by preventing heat-shock induction
with DnaK and DnaJ acts in response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins; may act as a thermosensor
DESCRIPTION:Gene expression, protein purification and characterization[PMID:10024560]
heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria
EXISTENCE:Gene expression[PMID:15758235]
catalyzes the formation of oxalozcetate and L-glutamate from L-aspartate and 2-oxoglutarate
catalyzes the formation of agmatine from arginine in putrescine and spermidine biosynthesis
histidyl-tRNA synthetase; catalyzes a two-step reaction, first charging a histidine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class II aminoacyl-tRNA synthetase; forms homodimers; some organisms have a paralogous gene, hisZ, that is similar to hisS and produces a protein that performs the first step in histidine biosynthesis along with HisG
catalyzes the reversible phosphoryl transfer from adenosine triphosphate (ATP) to thymidine monophosphate (dTMP) to form thymidine diphosphate (dTDP)
Catalyzes the conversion of ATP and pantetheine 4'-phosphate to diphosphate and 3'-dephospho-coA
3-polyprenyl-4-hydroxybenzoate decarboxylase
required for the assembly of the flagellar basal body P-ring
flagellar basal body P-ring biosynthesis protein FlgA
binding-protein dependent transport system permease
methionine ABC transporter ATP-binding protei
valyl-tRNA synthetase; ValRS; converts valine ATP and tRNA(Val) to AMP PPi and valyl-tRNA(Val); class-I aminoacyl-tRNA synthetase type 1 subfamily; has a posttransfer editing process to hydrolyze mischarged Thr-tRNA(Val) which is done by the editing domain
periplasmic; catalytic subunit; with NapBC catalyzes the reduction of nitrate to nitrite; NapAB receives electrons from NapC
part of NapHG quinol dehydrogenase; couples electron transfer from ubiquinone-ubiquinol couple via NapC/B to NapA; secreted by twin arginine translocation pathway
quinol dehydrogenase periplasmic subunit
part of NapHG quinol dehydrogenase; couples electron transfer from ubiquinone-ubiquinol couple via NapC/B to NapA
produces formate from formyl-tetrahydrofolate which is the major source of formate for PurT in de novo purine nucleotide biosynthesis; has a role in one-carbon metabolism; forms a homohexamer; activated by methionine and inhibited by glycine
DESCRIPTION:Gene expression, mutation analysis[PMID:1460570]
UDP-N-acetylmuramoyl-tripeptide D-alanyl-D-alanine ligase
D-alanyl-alanine synthetase A; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli
plays an essential role in ATP-dependent branch migration of the Holliday junction
cysteinyl-tRNA synthetase; catalyzes a two-step reaction; charges a cysteine by linking its carboxyl group to the alpha-phosphate of ATP then transfers the aminoacyl-adenylate to its tRNA
lipid ABC transporter ATP-binding protein/permease
catalyzes the conversion of dihydroorotate to orotate in the pyrimidine biosynthesis pathway; uses a flavin nucleotide as an essential cofactor; class 2 enzymes are monomeric and compared to the class 1 class 2 possess an extended N terminus, which plays a role in the membrane association of the enzyme and provides the binding site for the respiratory quinones that serve as physiological electron acceptors
catalyzes the formation of 4-hydroxy-tetrahydrodipicolinate from L-aspartate 4-semialdehyde and pyruvate in lysine and diaminopimelate biosynthesis
4-hydroxy-tetrahydrodipicolinate synthase
catalyzes the formation of nicotinamide adenine dinucleotide (NAD) from nicotinic acid adenine dinucleotide (NAAD) using either ammonia or glutamine as the amide donor and ATP; ammonia-utilizing enzymes include the ones from Bacillus and Escherichia coli while glutamine-utilizing enzymes include the Mycobacterial one; forms homodimers
transfers the gamma-phosphate of ATP to the 4' position of a tetraacyldisaccharide 1-phosphate intermediate to form tetraacyldisaccharide 1,4'-bis-phosphate
catalyzes the formation of L-threonine from O-phospho-L-homoserine
CMP-2-keto-3-deoxyoctulosonic acid synthetase; catalyzes the formation of CMP-3-deoxy-D-manno-octulosonate from CTP and 3-deoxy-D-manno-octulosonate which is incorporated into LPS
3-deoxy-manno-octulosonate cytidylyltransferase
FliP, with proteins FliQ and FliR, forms the core of the central channel in the flagella export apparatus
forms a homotrimer; catalyzes the acetylation of glucosamine-1-phosphate and uridylation of N-acetylglucosamine-1-phosphate to produce UDP-GlcNAc; function in cell wall synthesis
bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase
catalyzes the conjugation of cysteine to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, which is then decarboxylated to form 4'-phosphopantotheine
bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase
mediates pseudouridylation (positions 38, 39, 40) at the tRNA anticodon region which contributes to the structural stability
catalyzes the formation of 2-oxobutanoate from L-threonine
catalyzes the formation of 5-methyl-uridine at position 54 in all tRNAs
tRNA/tmRNA (uracil-C(5))-methyltransferase
catalyzes the conversion of citrate to isocitrate and the conversion of 2-methylaconitate to 2-methylisocitrate
methylated-DNA--protein-cysteine methyltransferase
methionyl-tRNA synthetase; MetRS; adds methionine to tRNA(Met) with cleavage of ATP to AMP and diphosphate; some MetRS enzymes form dimers depending on a C-terminal domain that is also found in other proteins such as Trbp111 in Aquifex aeolicus and the cold-shock protein CsaA from Bacillus subtilis while others do not; four subfamilies exist based on sequence motifs and zinc content
catalyzes the formation of D-fructose 6-phosphate from fructose-1,6-bisphosphate
molybdopterin-guanine dinucleotide biosynthesis protein
charges one glutamine molecule and pairs it with tRNA(Gln)
catalyzes the decarboxylaton of phospatidyl-L-sering to phosphatidylethanolamine
Converts (S)-4-amino-5-oxopentanoate to 5-aminolevulinate during the porphyrin biosynthesis pathway
glutamate-1-semialdehyde aminotransferase
catalyzes the formation of 5,10-methenyltetrahydrofolate from 5,10-methylenetetrahydrofolate and subsequent formation of 10-formyltetrahydrofolate from 5,10-methenyltetrahydrofolate
bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase
adds enolpyruvyl to UDP-N-acetylglucosamine as a component of cell wall formation; gram-positive bacteria have 2 copies of MurA which are active
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
para-aminobenzoate synthase glutamine amidotransferase subunit II
15 bp stem-loop; possible termination of replication?
membrane protein involved in the flagellar export apparatus
primary rRNA binding protein; helps nucleate assembly of 30S; binds directly to the 16S rRNA and an intersubunit bridge to the 23S rRNA; autoregulates translation through interactions with the mRNA leader sequence
tRNA Leu anticodon CAA, Cove score 75.00
tRNA Gly anticodon GCC, Cove score 88.10
sensory transduction transcriptional regulator
catalyzes the formation of 3-phosphonooxypyruvate from 3-phospho-D-glycerate in serine biosynthesis; can also reduce alpha ketoglutarate to form 2-hydroxyglutarate
in Escherichia coli this protein is involved in binding to the leader sequence of mRNAs and is itself bound to the 30S subunit; autoregulates expression via a C-terminal domain; in most gram negative organisms this protein is composed of 6 repeats of the S1 domain while in gram positive there are 4 repeats; the S1 nucleic acid-binding domain is found associated with other proteins
catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP); functions in the nonmevalonate isoprenoid biosynthesis pathway
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
catalyzes the formation of 5-O-(1-carboxyvinyl)-3-phosphoshikimate from phosphoenolpyruvate and 3-phosphoshikimate in tryptophan biosynthesis
3-phosphoshikimate 1-carboxyvinyltransferase
catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a tetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 2 subfamily
catalyzes a two-step reaction, first charging a phenylalanine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; forms a heterotetramer of alpha(2)beta(2); binds two magnesium ions per tetramer; type 1 subfamily
phenylalanine--tRNA ligase subunit alpha
4-methyl-5(beta-hydroxyethyl)-thiazole monophosphate synthesis protein
glutamine transporter ATP-binding protein
tRNA (cytidine(34)-2'-O)-methyltransferase
converts L-alanine to D-alanine which is used in cell wall biosynthesis; binds one pyridoxal phosphate per monomer; forms a homodimer
DESCRIPTION:Gene expression, mutation analysis[PMID:9034314]
EXISTENCE:Protein purification[PMID:8063109]
catalyzes the formation of 5-phospho-alpha-D-ribose 1-phosphate from D-ribose 5-phosphate and ATP
DESCRIPTION:Protein purification and characterization, mutation analysis[PMID:16689801]
DESCRIPTION:Protein purification and characterization[PMID:17631313]
bifunctional adhesin/ABC transporter aspartate/glutamate-binding protein
catalyzes a salvage reaction resulting in the formation of AMP which is metabolically less costly than a de novo synthesis
catalyzes the removal of N-terminal amino acids preferably leucine from various peptides
catalyzes the formation of arginine from (N-L-arginino)succinate
PEP carboxykinase; PEP carboxylase; PEPCK; catalyzes the phosphorylation and decarboxylation of oxaloacetate to form phosphoenolpyruvate using ATP
sodium:amino-acid symporter family protein
Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit C is part of the membrane proton channel F0
tRNA Leu anticodon GAG, Cove score 55.92
2-acylglycerophosphoethanolamine acyltransferase
tRNA Asp anticodon GTC, Cove score 91.78
tRNA Val anticodon TAC, Cove score 90.03
tRNA Lys anticodon TTT, Cove score 81.97
functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond SecA1; SecA2 is not essential and seems to play a role in secretion of a subset of proteins
participates with LolB in the incorporation of lipoprotein into the outer membrane
outer-membrane lipoprotein carrier protein
peptidyl-arginine deiminase family protein
bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
phosphoribosylformylglycinamidine synthase II
in Escherichia coli this protein is involved in the biosynthesis of the hypermodified nucleoside 5-methylaminomethyl-2-thiouridine, which is found in the wobble position of some tRNAs and affects ribosomal frameshifting; shows potassium-dependent dimerization and GTP hydrolysis; also involved in regulation of glutamate-dependent acid resistance and activation of gadE
functions to insert inner membrane proteins into the IM in Escherichia coli; interacts with transmembrane segments; functions in both Sec-dependent and -independent membrane insertion; similar to Oxa1p in mitochondria
membrane protein insertion efficiency factor
in Escherichia coli transcription of this gene is enhanced by polyamines
amino acid transporter substrate-binding protein
DESCRIPTION: similar to AA sequence:INSD:CAA85396.1
oxidoreductase ferredoxin-type electron transport protein
EXISTENCE:Protein crystalography[PMID:22949186]
catalyzes the formation of L-citrulline from carbamoyl phosphate and L-ornithine in arginine biosynthesis and degradation
catalyzes the formation of porphobilinogen from 5-aminolevulinate
catalyzes the conversion of GTP to formate and 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine and diphosphate
sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; this is the primary sigma factor of bacteria
phosphoglycerate/bisphosphoglycerate mutase
catalyzes the formation of 3-dehydroquinate from 3-deoxy-arabino-heptulonate 7-phosphate; functions in aromatic amino acid biosynthesis
Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr)
tRNA Arg anticodon GCG, Cove score 83.29
branched-chain amino acid ABC transporter permease
branched-chain amino acid ABC transporter substrate-binding protein
catalyzes the formation of 4-aspartyl phosphate from aspartate 4-semialdehyde
sigma-54 associated transcriptional activator
purine/pyrimidine phosphoribosyltransferase
GTP-binding protein LepA; binds to the ribosome on the universally-conserved alpha-sarcin loop
DESCRIPTION:Gene expression, mutation analysis[PMID:15793099]
multidrug efflux system outer membrane protein
EXISTENCE:Gene expression[PMID:16303882]
mutlidrug efflux system membrane fusion protein
DESCRIPTION:Gene expression, mutation analysis[PMID:16303882]
multidrug efflux system membrane protein
Conjugates Arg from its aminoacyl-tRNA to the N-termini of proteins containing an N-terminal aspartate or glutamate
biotin carboxylase; catalyses the carboxylation of the carrier protein which then transfers the carboxyl group to form malonyl-CoA, which in turn controls the rate of fatty acid metabolism
cell division/peptidoglycan biosynthesis protein
UDP-diphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase; involved in cell wall formation; inner membrane-associated; last step of peptidoglycan synthesis
UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
in Escherichia coli this enzyme functions in thiamine biosynthesis along with thiFSGI and iscS; with ThiFSG catalyzes the formation of thiazole phosphate from tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate; forms a complex with ThiG; contains an iron-sulfur center
functions in thiamine (vitamin B1) biosynthesis; in Bacillus subtilis this enzyme catalyzes the formation of thiazole from dehydroxyglycine and 1-deoxy-D-xylulose-5-phosphate and ThiS-thiocarboxylate
catalyzes the adenylation of ThiS which is involved in the formation of 5-methyl-4-(beta-hydroxyethyl)thiazole phosphate
dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE), catalyzes the hydrolysis of N-succinyl-L,Ldiaminopimelate L,L-SDAP to L,L-diaminopimelate and succinate. It is a metalloprotease containing dinuclear active sites. Its structure is similar to the carboxypeptidase G2 from Pseudomonas sp. strain RS-16 and the aminopeptidase from Aeromonas proteolytica.
recombination and DNA strand exchange inhibitor protein; MutS2; MutS-II; involved in blocking homologous and homeologous recombination; has ATPase activity stimulated by recombination intermediates; inhibits DNA strand exchange
Catalyzes the formation of UDP-N-acetylmuramoyl-L-alanine from UDP-N-acetylmuramate and L-alanine in peptidoglycan synthesis
carbon-nitrogen hydrolase family protein
catalyzes the synthesis of xanthosine monophosphate by the NAD+ dependent oxidation of inosine monophosphate
allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases; reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA
aspartyl/glutamyl-tRNA amidotransferase subunit A
isoleucyl-tRNA synthetase; IleRS; catalyzes the formation of isoleucyl-tRNA(Ile) from isoleucine and tRNA(Ile); since isoleucine and other amino acids such as valine are similar, there are additional editing function in this enzyme; one is involved in hydrolysis of activated valine-AMP and the other is involved in deacylation of mischarged Val-tRNA(Ile); there are two active sites, one for aminoacylation and one for editing; class-I aminoacyl-tRNA synthetase family type 1 subfamily; some organisms carry two different copies of this enzyme
DESCRIPTION:Gene expression, mutation analysis[PMID:1895937]
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase
binds cooperatively with S18 to the S15-16S complex, allowing platform assembly to continue with S11 and S21
binds to single stranded DNA and may facilitate the binding and interaction of other proteins to DNA
binds as a heterodimer with protein S6 to the central domain of the 16S rRNA; helps stabilize the platform of the 30S subunit
EXISTENCE:Gene expression[PMID:11895937]
binds to flagellin and appears to stabilize flagellin during flagella assembly
catalyzes the formation of L-proline from pyrroline-5-carboxylate
catalyzes the formation of uroporphyrinogen-III from hydroxymethylbilane; functions in tetrapyrrole and heme biosynthesis
bifunctional folylpolyglutamate synthase/dihydrofolate synthase
leucyl-tRNA synthetase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl-tRNA synthetase does not recognize the anticodon loop of the tRNA, but instead recognition is dependent on a conserved discriminator base A37 and a long arm; an editing domain hydrolyzes misformed products; in Methanothermobacter thermautotrophicus this enzyme associates with prolyl-tRNA synthetase
forms a complex with SecD and YajC; SecDFyajC stimulates the proton motive force-driven protein translocation; seems to modulate the cycling of SecA by stabilizing its membrane-inserted state and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane; in some organisms, such as Bacillus subtilis, SecD is fused to SecF
part of the preprotein secretory system; when complexed with proteins SecF and YajC, SecDFyajC stimulates the proton motive force-driven protein translocation, and appears to be required for the release of mature proteins from the extracytoplasmic side of the membrane
member of preprotein translocase; forms a heterotrimer with SecD and SecF; links the SecD/SecF/YajC/YidC complex with the SecY/SecE/SecG complex
Transfers the fatty acyl group on membrane lipoproteins
catalyzes the formation of S-adenosylmethionine from methionine and ATP; methionine adenosyltransferase
involved in the import of serine and threonine coupled with the import of sodium
catalyzes the transfer of the carbamoyl moiety from carbamoyl phosphate to L- aspartate in pyrimidine biosynthesis
catalyzes isomerization of specific uridines in RNA to pseudouridine; responsible for residues in T loops of many tRNAs
catalyzes the phosphorylation of 4-diphosphocytidyl-2-C-methyl-D-erythritol in the nonmevalonate pathway of isoprenoid biosynthesis
4-diphosphocytidyl-2C-methyl-D-erythritol kinase
binds to ssrA RNA (tmRNA) and is required for its successful binding to ribosomes; also appears to function in the trans-translation step by promoting accommodation of tmRNA into the ribosomal A site; SmpB protects the tmRNA from RNase R degradation in Caulobacter crescentus; both the tmRNA and SmpB are regulated in cell cycle-dependent manner; functions in release of stalled ribosomes from damaged mRNAs and targeting proteins for degradation
ATP-dependent Clp protease adaptor protein
ATP-dependent Clp protease ATP-binding subunit
leucyltransferase; phenylalanyltransferse; functions in the N-end rule pathway; transfers Leu, Phe, Met, from aminoacyl-tRNAs to N-terminal of proteins with Arg or Lys
leucyl/phenylalanyl-tRNA--protein transferase
this stereospecific enzymes reduces the R isomer of methionine sulfoxide while MsrA reduces the S form; a fusion protein of this enzyme with MsrA and thioredoxin provides protection against oxidative stress in Neisseria gonorrhoeae
CDP-diacylglycerol--serine O-phosphatidyltransferase
phosphatidylserine decarboxylase-like protein
methylates ribosomal protein L11 at multiple amino acid positions; mutations of these genes in Escherichia coli or Thermus thermophilus has no apparent phenotype
50S ribosomal protein L11 methyltransferase
DESCRIPTION: similar to AA sequence:INSD:AAC44858.1
DESCRIPTION:Protein purification and characterization[PMID:16286454]
UDP-N-acetyl-alpha-D-glucosamine C6 dehydratase
DESCRIPTION:Protein purification and characterization, mutation analysis[PMID:18198901]
DESCRIPTION:Protein purification and characterization[PMID:17087520]
UDP-N-acetylbacillosamine N-acetyltransferase
DESCRIPTION:Protein purification and characterization[PMID:16618123]
undecaprenyl phosphate N,N'-diacetylbacillosamine 1-phosphate transferase
DESCRIPTION:Protein purification and characterization[PMID:16186480]
N, N'-diacetylbacillosaminyl-diphospho-undecaprenol alpha-1,3-N-acetylgalactosaminyltransferase
DESCRIPTION:Gene expression[PMID:16356848]
DESCRIPTION:Protein purification and characterization[PMID:20331969]
undecaprenyl-diphosphooligosaccharide--protein glycotransferase
DESCRIPTION:Protein purification and characterization[PMID:18034500]
N-acetylgalactosamine-N, N'-diacetylbacillosaminyl-diphospho-undecaprenol 4-alpha-N-acetylgalactosaminyltransferase
DESCRIPTION:Gene expression, mutation analysis[PMID:16547029]
GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase
DESCRIPTION:Protein purification and characterization[PMID:19159314]
GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase
COORDINATES:Gene expression, mutation analysis[PMID:16547029]
DESCRIPTION:Gene expression, protein purification and characterization[PMID:16498400]
DESCRIPTION:Gene expression, mutation analysis, protein purification and characterization[PMID:15509570]
DESCRIPTION: similar to AA sequence:INSD:AAF34138.2
acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA; essential for survival; plays a role in cell responses to environmental changes
lipid A biosynthesis lauroyl acyltransferase
DESCRIPTION:Gene expression, mutation analysis[PMID:101931344]
DESCRIPTION:Gene expression, mutation analysis[PMID:10712693]
DESCRIPTION:Gene expression, mutation analysis[PMID:11689567]
bifunctional beta-1,4-N-acetylgalactosaminyltransferase/CMP-Neu5Ac synthase
DESCRIPTION:Gene expression, mutation analysis[PMID:11914340]
DESCRIPTION::Gene expression, mutation analysis[PMID:15612919]
catalyzes the isomerization of sedoheptulose 7-phosphate to D-glycero-D-manno-heptose 7-phosphate
bifunctional D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase
DESCRIPTION:Gene expression, mutation analysis[PMID:15612919]
D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase
cbb3-type cytochrome oxidase maturation protein
An RNA-DNA helicase that actively releases nascent mRNAs from paused transcription complexes
catalyzes the DNA-template-directed extension of the 3'-end of a DNA strand; the tau chain serves as a scaffold to help in the dimerizaton of the alpha,epsilon and theta core complex; the gamma chain seems to interact with the delta and delta' subunits to transfer the beta subunit on the DNA
DNA polymerase III subunits gamma and tau
heavy-metal-associated domain-containing protein
DESCRIPTION:Gene expression, protein purification and characterization[PMID:11104668]
arginyl-tRNA synthetase; catalyzes a two-step reaction, first charging an arginine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA synthetase
Essential for recycling GMP and indirectly, cGMP
FliR, with proteins FliP and FliQ, forms the core of the central channel in the flagella export apparatus
EF-Ts; functions during elongation stage of protein translation; forms a dimer; associates with EF-Tu-GDP complex and promotes exchange of GDP to GTP resulting in regeneration of the active form of EF-Tu
one of the last subunits in the assembly of the 30S subunit; absence of S2 does not inhibit assembly but results in an inactive subunit
cyclopropane-fatty-acyl-phospholipid synthase
EXISTENCE:Gene expression[PMID:16041056]
ubiquinol-cytochrome C reductase cytochrome C subunit
ubiquinol-cytochrome C reductase cytochrome B subunit
ubiquinol-cytochrome C reductase iron-sulfur subunit
GidA; glucose-inhibited cell division protein A; involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U) of the wobble uridine base in some tRNAs
tRNA uridine 5-carboxymethylaminomethyl modification protein GidA
DESCRIPTION:Gene expression, mutation analysis[PMID:11289288]
bipartate energy taxis response protein CetB
bipartate energy taxis response protein CetA
PAS domain-containing signal-transduction sensor protein
Catalyzes the reversible hydrolysis of the amide bond within dihydroorotate. This metabolic intermediate is required for the biosynthesis of pyrimidine nucleotides
catalyzes the NAD(P)H-dependent reduction of glycerol 3-phosphate to glycerone phosphate
aspartyl/glutamyl-tRNA amidotransferase subunit B
DESCRIPTION:Gene expression, mutation analysis[PMID:11988522]
catalyzes the hydrolysis of S-ribosylhomocysteine to homocysteine and autoinducer-2
catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine to form methionine
5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase
Produces ATP from ADP in the presence of a proton gradient across the membrane. Subunit A is part of the membrane proton channel F0
Sms; stabilizes the strand-invasion intermediate during the DNA repair; involved in recombination of donor DNA and plays an important role in DNA damage repair after exposure to mutagenic agents
catalyzes the formation of riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine
EXISTENCE:Gene expression[PMID:10206714]
10 kDa chaperonin; Cpn10; GroES; forms homoheptameric ring; binds to one or both ends of the GroEL double barrel in the presence of adenine nucleotides capping it; folding of unfolded substrates initiates in a GroEL-substrate bound and capped by GroES; release of the folded substrate is dependent on ATP binding and hydrolysis in the trans ring
60 kDa chaperone family; promotes refolding of misfolded polypeptides especially under stressful conditions; forms two stacked rings of heptamers to form a barrel-shaped 14mer; ends can be capped by GroES; misfolded proteins enter the barrel where they are refolded when GroES binds; many bacteria have multiple copies of the groEL gene which are active under different environmental conditions; the B.japonicum protein in this cluster is expressed constitutively; in Rhodobacter, Corynebacterium and Rhizobium this protein is essential for growth
DESCRIPTION:Gene expression, mutation analysis[PMID:15554967]
tRNA Asn anticodon GTT, Cove score 84.50
DESCRIPTION:Gene expression, mutation analysis[PMID:15933023]
DESCRIPTION:Gene expression, mutation analysis[PMID:15758235]
glutathione-regulated potassium-efflux system protein
glycyl-tRNA synthetase beta chain; glyS; class II aminoacyl tRNA synthetase; tetramer of alpha(2)beta(2); catalyzes a two-step reaction; first charging a glycine molecule by linking the carboxyl group to the alpha-phosphate of ATP; second by transfer of the aminoacyl-adenylate to its tRNA
involved in the de novo synthesis of pyridoxine (Vitamin B6)
catalyzes oxidation of 4-(phosphohydroxy)-L-threonine into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which decarboxylates to form 1-amino-3-(phosphohydroxy)propan-2-one (3-amino-2-oxopropyl phosphate)
4-hydroxythreonine-4-phosphate dehydrogenase
catalyzes the formation of coproporphyrinogen from uroporphyrinogen III
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision
contains glutamine-hydrolyzing domain and glutamine amidotransferase; GMP-binding domain; functions to produce GMP from XMP in the IMP pathway
catalyzes the formation of N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-D-ribosylamine and glycine in purine biosynthesis
DESCRIPTION:Protein purification and characterization[PMID:7543469]
chaperone Hsp40; co-chaperone with DnaK; Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion
DESCRIPTION: similar to AA sequence:INSD:AAC08021.1
involved in a recombinational process of DNA repair, independent of the recBC complex
Ni/Fe-hydrogenase B-type cytochrome subunit
bifunctional tRNA (mnm(5)s(2)U34)-methyltransferase/FAD-dependent cmnm(5)s(2)U34 oxidoreductase
tyrosyl-tRNA synthetase; catalyzes the formation of tyrosyl-tRNA(Tyr) from tyrosine and tRNA(Tyr)
guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits
DNA-directed RNA polymerase subunit omega
Catalyzes the phosphorylation of UMP to UDP
tRNA (guanine-N(7)-)-methyltransferase; catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA by transferring the methyl residue from S-adenosyl-L-methionine
fibronectin domain-containing lipoprotein
tRNA Met anticodon CAT, Cove score 97.76
tRNA Phe anticodon GAA, Cove score 85.44
Catalyzes the formation of uracil and 5-phospho-alpha-D-ribosy 1-diphosphate from UMP and diphosphate
glutamyl-tRNA synthetase; charges one glutamine molecule and pairs it to its corresponding RNA trinucleotide during protein translation
biotin carboxylase; biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA; catalyzes the formation of malonyl-CoA, which in turn controls the rate of fatty acid metabolism
acetyl-CoA carboxylase, biotin carboxyl carrier protein
Catalyzes the formation of dUTP from dCTP in thymidylate biosynthesis
DESCRIPTION:Gene expression, mutation analysis[PMID:14617187]
DESCRIPTION:Protein purification and characterization[PMID:14960321]
Cj1293 is involved in flagellin glycosylation and has USP-GlcNAc C(6) dehydratase activity. This enzyme catalyzes the first step in the biosynthesis of bacillosamine, a sugar found in Campylobacter jejuni glycosylation motifs; found to exhibit C6 dehydratase; C5 epimerase activity; plays a direct role in CMP-Pse5NAc7NAc or CMP-Pse5NAc7Am pathyways.
DESCRIPTION:Protein purification and characterization[PMID:15790564]
pyridoxal phosphate-dependent aminotransferase specific for UDP-4-keto-6-deoxy-GlcNAc. These results indicate that Cj1294 is involved in the biosynthesis of diacetamidofucosamine, a C4 epimer of diacetamidobacillosamine; utilizes UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose as substrate producing UDP-4-amino-4,6-dideoxy-beta-L-AltNAc; plays a direct role in CMP-Pse5NAc7NAc or CMP-Pse5NAc7Am pathyways
UDP-4-amino-4, 6-dideoxy-N-acetyl-beta-L-altrosamine transaminase
DESCRIPTION:Protein purification and characterization[PMID:19282391]
FabH; beta-ketoacyl-acyl carrier protein synthase III; catalyzes the condensation of acetyl-CoA with malonyl-ACP to initiate cycles of fatty acid elongation; differs from 3-oxoacyl-(acyl carrier protein) synthase I and II in that it utilizes CoA thioesters as primers rather than acyl-ACPs; paralogs which do not contain the N-X-R ACP-binding site motif
DESCRIPTION:Protein purification and characterization[PMID:16728396]
UDP-2,4-diacetamido-2,4, 6-trideoxy-beta-L-altropyranose hydrolase
catalyzes the conversion of 5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]- 1-(5-phosphoribosyl)imidazole-4-carboxamide and glutamine to imidazole-glycerolphosphate, 5-aminoimidazol-4-carboxamideribonucleotide and glutamate; the HisF subunit acts as a cyclase
imidazole glycerol phosphate synthase subunit HisF
with HisF IGPS catalyzes the conversion of phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide ribonucleotide phosphate and glutamine to imidazole-glycerol phosphate, 5-aminoimidazol-4-carboxamide ribonucleotide, and glutamate in histidine biosynthesis; the HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of imidazole-glycerol phosphate and 5-aminoimidazol-4-carboxamide ribonucleotide
imidazole glycerol phosphate synthase subunit HisH
DESCRIPTION:Gene expression, mutation analysis[PMID:11461915]
DESCRIPTION:Gene expression, mutation analysis[PMID:16573681]
PseA has been proposed to play a role in the pseudaminic acid biosynthetic pathway by appearing to synthesize pseudaminic acid (PseAm) directly from PseAc by transfer of an acetamidino group. Plays a direct role in CMP-Pse5NAc7NAc or CMP-Pse5NAc7Am biosynthesis or transfer.
pseudaminic acid biosynthesis protein PseA
DESCRIPTION:Protein purification and characterization[PMID:16120604]
repeat 3; identical to 1263498..1265455 (approx. positions)
GDP/UDP-N,N'-diacetylbacillosamine 2-epimerase
CMP-N,N'-diacetyllegionaminic acid synthase
DESCRIPTION:Gene expression, mutation analysis[PMID:11895937]
DESCRIPTION:Gene expression, mutation analysis[PMID:16673682]
repeat 3; identical to 1246669..1248628 (approx. positions)
FlaB; structural flagella protein; in Helicobacter the flagella are composed of flagellin A and flagellin B; the amounts of each seem to be controlled by environmental conditions
FlaA; structural flagella protein; in Helicobacter the flagella are composed of flagellin A and flagellin B; the amounts of each seem to be controlled by environmental conditions
in most organisms, only the N-terminal domain is present in a single polypeptide; in some archaea this domain is fused to a kinase domain; this gene is essential for growth in Escherichia coli and Bacillus subtilis; the secreted glycoprotease from Pasteurella haemolytica showed specificity for O-sialoglycosylated proteins; the Pyrococcus structure shows DNA-binding properties, iron-binding, ATP-binding, and AP endonuclease activity
tRNA N6-adenosine threonylcarbamoyltransferase
catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate
1-deoxy-D-xylulose 5-phosphate reductoisomerase
DESCRIPTION: similar to AA sequence:INSD:CAA71915.1
DESCRIPTION: similar to AA sequence:INSD:CAA61301.1
DESCRIPTION: similar to AA sequence:INSD:CAA61302.1
DESCRIPTION: similar to AA sequence:INSD:CAA61303.1
DESCRIPTION: similar to AA sequence:INSD:CAA61304.1
enterochelin uptake substrate-binding protein
tRNA Ser anticodon GCT, Cove score 68.31
cytochrome c nitrite reductase cytochrome c552 subunit
cytochrome c nitrite reductase small subunit
catalyzes the reversible transfer of the terminal phosphate of ATP to form a long chain polyphosphate
10Sa RNA (tmRNA), Proteolysis Tag: (A)ANNVKFAPAYAKAA; Updated (2006) note: Rfam domain RF00023, tmRNA also identified within CDS. Further support given to product function
promotes strand exchange during homologous recombination; RuvAB complex promotes branch migration; RuvABC complex scans the DNA during branch migration and resolves Holliday junctions at consensus sequences; forms hexameric rings around opposite DNA arms; requires ATP for branch migration and orientation of RuvAB complex determines direction of migration
class II family (does not require metal); tetrameric enzyme; fumarase C; reversibly converts (S)-malate to fumarate and water; functions in the TCA cycle
Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source
glucosamine--fructose-6-phosphate aminotransferase
HAM1-like protein; Rec-dependent growth; RgdB; yggV; it is suspected that this protein functions to remove misincorporated bases such as xanthine or hypoxanthine
Non-canonical purine NTP pyrophosphatase
catalyzes the formation of selenocysteinyl-tRNA(Sec) from seryl-tRNA(Sec) and L-selenophosphate in selenoprotein biosynthesis
selenocysteine-specific elongation factor
An electron-transfer protein; flavodoxin binds one FMN molecule, which serves as a redox-active prosthetic group
DESCRIPTION:Gene expression, mutation analysis[PMID:7670638]
EXISTENCE:Gene expression[PMID:14735223]
NADH; Catalyzes a key regulatory step in fatty acid biosynthesis
Reversibly isomerizes the ketone sugar dihydroxyacetone phosphate to the aldehyde sugar glyceraldehyde-3-phosphate
Converts 3-phospho-D-glycerate to 3-phospho-D-glyceroyl phosphate during the glycolysis pathway
glyceraldehyde 3-phosphate dehydrogenase
nicotinate-nucleotide adenylyltransferase
interacts with the cytoplasmic MS ring of the basal body and may act to stabilize the MotAB complexes which surround the MS ring
flagellar basal body-associated protein FliL
Catalyzes the formation of holo-ACP, which mediates the essential transfer of acyl fatty acid intermediates during the biosynthesis of fatty acids and lipids
DESCRIPTION:Gene expression, mutation analysis[PMID:10672176]
capsule polysaccharide modification protein
converts ATP and adenylyl sulfate to ADP and 3'-phosphoadenylyl sulfate; in Escherichia coli this enzyme functions in cysteine biosynthesis
D-glycero-D-manno-heptose 1-phosphate guanosyltransferase
D-glycero-D-manno-heptose 7-phosphate kinase
DESCRIPTION:Gene expression, mutation analysis, protein localization[PMID:12392544]
capsular polysaccharide heptosyltransferase
capsule polysaccharide ABC transporter substrate-binding protein
capsule polysaccharide ABC transporter permease
DESCRIPTION: similar to AA sequence:INSD:AAK62274.1
capsule polysaccharide ABC transporter ATP-binding protein
EXISTENCE:Gene expression[PMID:16740937]
DESCRIPTION:Protein purification and characterization[PMID:15364583]
ribosomal protein S12 methylthiotransferase
recognizes the termination signals UGA and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF2; in some organisms control of PrfB protein levels is maintained through a +1 ribosomal frameshifting mechanism; this protein is similar to release factor 1
catalyzes the modification of U13 in tRNA(Glu)
catalyzes the formation of thiamine diphosphate from thiamine phosphate ant ATP
part of the basal body which consists of four rings L, P, S, and M mounted on a central rod; Vibrio parahaemolyticus, Yersinia, Bradyrhizobium and other bacteria have two copies of this and other flagellar genes; the V. parahaemolyticus protein is associated with the polar flagella and the Bradyrhizobium protein is associated with the thick flagellum
EXISTENCE:Gene expression[PMID:14960570]
with FlgL acts as a hook filament junction protein to join the flagellar filament to the hook
type II protein secretion system protein E
type II protein secretion system protein D
DESCRIPTION: similar to AA sequence:INSD:AAC35418.1
outer membrane fibronectin-binding protein
forms a direct contact with the tRNA during translation
in Escherichia coli this protein is one of the earliest assembly proteins in the large subunit
cbb3-type cytochrome C oxidase subunit III
cbb3-type cytochrome C oxidase subunit IV
cbb3-type cytochrome c oxidase subunit II
cbb3-type cytochrome c oxidase subunit I
catalyzes production of carbamoyl phosphate from bicarbonate and glutamine in pyrimidine and arginine biosynthesis pathways; forms an octamer composed of four CarAB dimers
carbamoyl phosphate synthase small subunit
catalyzes the formation of N6-(1,2,-dicarboxyethyl)-AMP from L-aspartate, inosine monophosphate and GTP in AMP biosynthesis
tRNA SeC anticodon TCA, Cove score 35.94
proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase
involved in the production or activity of formate dehydrogenase-H which is active when nitrate is not present during anaerobic growth
formate dehydrogenase cytochrome B subunit
formate dehydrogenase iron-sulfur subunit
carboxynorspermidine/carboxyspermidine decarboxylase
molybdopterin converting factor subunit 1
molybdopterin converting factor subunit 2
CRISPR; Updated (2006) note: Cj1520 has now been removed due to identification of CRISPR repeat region; PMID:11952905, PMID:16612537, PMID:16545108'
catalyzes the formation of 1-(5-phosphoribosyl)-5-aminoimidazole from 2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in purine biosynthesis
phosphoribosylformylglycinamidine cyclo-ligase
catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A; involved in coenzyme A biosynthesis
involved in lysine biosynthesis; DAP epimerase; produces DL-diaminopimelate from LL-diaminopimelate
catalyzes the formation of D-fructose 6-phosphate from D-glucose 6-phosphate
UTP-glucose-1-phosphate uridylyltransferase
Acs; catalyzes the conversion of acetate and CoA to acetyl-CoA
anion uptake ABC transporter ATP-binding protein
repeat 1: identical to 147606..148684 and complement(238817..239964) (approximate numbers)
DESCRIPTION: similar to AA sequence:INSD:AAA86822.1
Catalyzes the transfer of electrons from NADH to ubiquinone
Catalyzes the transfer of electrons from NADH to quinone
The point of entry for the majority of electrons that traverse the respiratory chain eventually resulting in the reduction of oxygen
peptide ABC transporter ATP-binding protein
peptide ABC transporter substrate-binding protein
DESCRIPTION:Gene expression, mutation analysis[PMID:15292134]
efflux pump for the antibacterial peptide microcin J25
multidrug ABC transporter permease/ATP-binding protein
stimulates the activities of the other two initiation factors, IF-2 and IF-3
smallest protein in the large subunit; similar to what is found with protein L31 and L33 several bacterial genomes contain paralogs which may be regulated by zinc; the protein from Thermus thermophilus has a zinc-binding motif and contains a bound zinc ion; the proteins in this group have the motif
located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA; makes contact with the large subunit via RNA-protein interactions and via protein-protein interactions with L5; contacts P-site tRNA
located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA; forms part of the Shine-Dalgarno cleft in the 70S ribosome; interacts with S7 and S18 and IF-3
primary rRNA binding protein; nucleates 30S assembly; involved in translational accuracy with proteins S5 and S12; interacts with protein S5; involved in autogeneously regulating ribosomal proteins by binding to pseudoknot structures in the polycistronic mRNA; interacts with transcription complex and functions similar to protein NusA in antitermination
catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Dimerization of the alpha subunit is the first step in the sequential assembly of subunits to form the holoenzyme
DNA-directed RNA polymerase subunit alpha
is a component of the macrolide binding site in the peptidyl transferase center
long form of enzyme; catalyzes the formation of N'-5'-phosphoribosyl-ATP from phosphoribosyl pyrophosphate; crucial role in histidine biosynthesis; forms active dimers and inactive hexamers which is dependent on concentration of substrates and inhibitors
catalyzes the oxidation of L-histidinol to L-histidinaldehyde and then to L-histidine in histidine biosynthesis; functions as a dimer
catalyzes the formation of 3-(imidazol-4-yl)-2-oxopropyl phosphate from D-ethythro-1-(imidazol-4-yl)glycerol 3-phosphate and histidinol from histidinol phosphate
bifunctional imidazole glycerol-phosphate dehydratase/histidinol phosphatase
catalyzes the formation of 5-(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino-l- (5-phosphoribosyl)imidazole-4-carboxamide from 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide
1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
catalyzes the conversion of 5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]- 1-(5-phosphoribosyl)imidazole-4-carboxamideand glutamine to imidazole-glycerol phosphate, 5-aminoimidazol-4-carboxamideribonucleotide and glutamate; the HisF subunit acts as a cyclase
catalyzes the formation of 1-(5-phosphoribosyl)-AMP from 1-(5-phosphoribosyl)-ATP and the subsequent formation of 1-(5-phosphoribosyl)-5-((5- phosphoribosylamino)methylideneamino)imidazole-4- carboxamide from 1-(5-phosphoribosyl)-AMP in histidine biosynthesis
bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
DESCRIPTION:Gene expression[PMID:15233799]
bifunctional enzyme involved in formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate and 2-C-methyl-D-erythritol 2,4-cyclodiphosphate and CMP from 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate; involved in isoprenoid and isopentenyl-PP biosynthesis; binds divalent cations
bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
binds directly to the 16S rRNA and is involved in post-translational inhibition of arginine and ornithine decarboxylase
recognizes the termination signals UAG and UAA during protein translation a specificity which is dependent on amino acid residues residing in loops of the L-shaped tRNA-like molecule of RF1; this protein is similar to release factor 2
EXISTENCE:Protein purification[PMID:9765558]
hemin uptake system outer membrane receptor
hemin uptake ABC transporter ATP-binding protein
hemin uptake system substrate-binding protein
tRNA Leu anticodon TAA, Cove score 69.07
tRNA Cys anticodon GCA, Cove score 72.19
tRNA Ser anticodon TGA, Cove score 68.51
catalyzes the formation of chorismate from 5-O-(1-carboxyvinyl)-3-phosphoshikimate in aromatic amino acid biosynthesis
cytoplasmic enzyme involved in processing rRNA and some mRNAs; substrates typically have dsRNA regions; forms a homodimer; have N-terminal nuclease and C-terminal RNA-binding domains; requires magnesium as preferred ion for activity
An endonuclease that specifically degrades the RNA strand of RNA-DNA hybrids
synthesizes RNA primers at the replication forks
involved in cell wall formation; peptidoglycan synthesis; cytoplasmic enzyme; catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-aceylmuramoyl-l-alanyl-d-glutamate
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase
catalyzes the formation of ribose 5-phosphate and xylulose 5-phosphate from sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate; can transfer ketol groups between several groups; in Escherichia coli there are two tkt genes, tktA expressed during exponential growth and the tktB during stationary phase
catalyzes the removal of N-terminal amino acids from peptides and arylamides; generally Co(II) however activity has been shown for some methionine aminopeptidases with Zn, Fe, or Mn
converts L-glutamate to D-glutamate, a component of peptidoglycan
exports sodium by using the electrochemical proton gradient to allow protons into the cell; functions in adaptation to high salinity and alkaline pH; activity increases at higher pH; downregulated at acidic pH
DESCRIPTION: similar to AA sequence:INSD:CAA73983.1
tRNA Leu anticodon TAG, Cove score 76.79
tRNA Arg anticodon TCT, Cove score 87.94
tRNA Arg anticodon TCG, Cove score 83.86
tRNA His anticodon GTG, Cove score 77.53
tRNA Pro anticodon TGG, Cove score 88.47
catalyzes the formation of a phosphodiester at the site of a single-strand break in duplex DNA
DESCRIPTION:Mutation analysis, protein purification and characterization[PMID:11985725]
phosphopyruvate hydratase; catalyzes the formation of phosphoenolpyruvate from 2-phospho-D-glycerate in glycolysis
catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs
with proteins FliP and FliR forms the core of the central channel in the flagella export apparatus
catalyzes the reduction of UDP-N-acetylglucosamine enolpyruvate to form UDP-N-acetylmuramate in peptidoglycan biosynthesis
UDP-N-acetylenolpyruvoylglucosamine reductase
3'(2'),5'-bisphosphate nucleotidase CysQ
catalyzes the formation of biotin from dethiobiotin and sulfur
catalyzes the ATP-dependent breakage of single-stranded DNA followed by passage and rejoining, maintains net negative superhelicity
EXISTENCE:Gene expression[PMID:16048946]
forms heterotrimeric complex in the membrane; in bacteria the complex consists of SecY which forms the channel pore and SecE and SecG; the SecG subunit is not essential; in bacteria translocation is driven via the SecA ATPase
located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body; contacts S4 and S8; with S4 and S12 plays a role in translational accuracy; mutations in this gene result in spectinomycin resistance
binds 5S rRNA along with protein L5 and L25
ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance
binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit
located in the peptidyl transferase center and involved in assembly of 30S ribosome subunit; similar to what is observed with proteins L31 and L33, some proteins in this family contain CXXC motifs that are involved in zinc binding; if two copies are present in a genome, then the duplicated copy appears to have lost the zinc-binding motif and is instead regulated by zinc; the proteins in this group appear to contain the zinc-binding motif
part of 50S and 5S/L5/L18/L25 subcomplex; contacts 5S rRNA and P site tRNA; forms a bridge to the 30S subunit in the ribosome by binding to S13
assembly initiator protein; binds to 5' end of 23S rRNA and nucleates assembly of the 50S; surrounds polypeptide exit tunnel
binds to the 23S rRNA between the centers for peptidyl transferase and GTPase
primary binding protein; helps mediate assembly; involved in translation fidelity
one of the stabilizing components for the large ribosomal subunit
located in the peptidyl transferase center and may be involved in peptidyl transferase activity; similar to archaeal L10e
forms a complex with S10 and S14; binds the lower part of the 30S subunit head and the mRNA in the complete ribosome to position it for translation
binds specifically to 23S rRNA during the early stages of 50S assembly; makes contact with all 6 domains of the 23S rRNA in the assembled 50S subunit and ribosome; mutations in this gene result in erythromycin resistance; located near peptidyl-transferase center
protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA
one of the primary rRNA-binding proteins; required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation
binds third domain of 23S rRNA and protein L29; part of exit tunnel
L4 is important during the early stages of 50S assembly; it initially binds near the 5' end of the 23S rRNA
binds directly near the 3' end of the 23S rRNA, where it nucleates assembly of the 50S subunit; essential for peptidyltransferase activity; mutations in this gene confer resistance to tiamulin
NusE; involved in assembly of the 30S subunit; in the ribosome, this protein is involved in the binding of tRNA; in Escherichia coli this protein was also found to be involved in transcription antitermination; NusB/S10 heterodimers bind boxA sequences in the leader RNA of rrn operons which is required for antitermination; binding of NusB/S10 to boxA nucleates assembly of the antitermination complex
catalyzes the transfer of a total of four methyl groups from S-adenosyl-l-methionine (S-AdoMet) to two adjacent adenosine bases A1518 and A1519 in 16S rRNA; mutations in ksgA causes resistance to the translation initiation inhibitor kasugamycin
23S rRNA m2A2503 methyltransferase; methylates the C2 position of the A2530 nucleotide in 23S rRNA; may be involved in antibiotic resistance
23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN
tRNA Met anticodon CAT, Cove score 81.03
tRNA Ser anticodon GGA, Cove score 63.73
tRNA Ala anticodon GGC, Cove score 84.81
tRNA Val anticodon GAC, Cove score 82.27
catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate in leucine biosynthesis; forms a heterodimer of LeuC/D
3-isopropylmalate dehydratase small subunit
dehydratase component, catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate
3-isopropylmalate dehydratase large subunit
catalyzes the oxidation of 3-isopropylmalate to 3-carboxy-4-methyl-2-oxopentanoate in leucine biosynthesis
catalyzes the formation of 2-isopropylmalate from acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis
NADPH-dependent; catalyzes the reduction of 7-cyano-7-deazaguanine to 7-aminomethyl-7-deazaguanine in queuosine biosynthesis
catalyzes the formation of O-succinyl-L-homoserine from succinyl-CoA and L-homoserine in methionine biosynthesis
the hook connects flagellar basal body to the flagellar filament
endonuclease; resolves Holliday structures; forms a complex of RuvABC; the junction binding protein RuvA forms a hexameric ring along with the RuvB helicase and catalyzes branch migration; RuvC then interacts with RuvAB to resolve the Holliday junction by nicking DNA strands of like polarity