Kitasatospora aureofaciens strain DM-1 plasmid unnamed1, complete sequence.
Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales; Streptomycetaceae; Kitasatospora.
TAR Cloning and Integrated Overexpression of 6-demethylchlortetracycline Biosynthetic Gene Cluster in Streptomyces aureofaciens
Submitted (03-APR-2017) 1. Department of Pharmacology 2. Department of Biopharmceutical, 1. School of Pharmacy, Fudan University 2.Shanghai Institute of Pharmaceutical Industry, No. 826 Zhangheng Road Pudong District, Shanghai, Shanghai 201203, China
REFSEQ INFORMATION: The reference sequence is identical to\nCP020568.1.\nThe annotation was added by the NCBI Prokaryotic Genome Annotation\nPipeline (PGAP). Information about PGAP can be found here:\nhttps://www.ncbi.nlm.nih.gov/genome/annotation_prok/\n\n##Genome-Assembly-Data-START##\nAssembly Method :: Newbler\nGenome Coverage :: 23\nSequencing Technology :: 454\n##Genome-Assembly-Data-END##\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: NCBI RefSeq\nAnnotation Date :: 02/10/2023 17:41:36\nAnnotation Pipeline :: NCBI Prokaryotic Genome\n Annotation Pipeline (PGAP)\nAnnotation Method :: Best-placed reference protein\n set; GeneMarkS-2+\nAnnotation Software revision :: 6.4\nFeatures Annotated :: Gene; CDS; rRNA; tRNA; ncRNA\nGenes (total) :: 6,286\nCDSs (total) :: 6,186\nGenes (coding) :: 5,997\nCDSs (with protein) :: 5,997\nGenes (RNA) :: 100\nrRNAs :: 9, 9, 9 (5S, 16S, 23S)\ncomplete rRNAs :: 9, 9, 9 (5S, 16S, 23S)\ntRNAs :: 70\nncRNAs :: 3\nPseudo Genes (total) :: 189\nCDSs (without protein) :: 189\nPseudo Genes (ambiguous residues) :: 0 of 189\nPseudo Genes (frameshifted) :: 62 of 189\nPseudo Genes (incomplete) :: 151 of 189\nPseudo Genes (internal stop) :: 13 of 189\nPseudo Genes (multiple problems) :: 37 of 189\nCRISPR Arrays :: 6\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
COORDINATES: ab initio prediction:GeneMarkS-2+
Derived by automated computational analysis using gene prediction method: GeneMarkS-2+.
COORDINATES: similar to AA sequence:RefSeq:WP_014133191.1
Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: protein motif:HMM:NF024767.2
COORDINATES: similar to AA sequence:RefSeq:WP_008736052.1
GO:0003700 - DNA-binding transcription factor activity [Evidence IEA]
GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]
COORDINATES: protein motif:HMM:NF017542.2
GO:0006260 - DNA replication [Evidence IEA]; GO:0006276 - plasmid maintenance [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_018635389.1
COORDINATES: protein motif:HMM:NF013677.2
GO:0003677 - DNA binding [Evidence IEA]; GO:0004803 - transposase activity [Evidence IEA]
GO:0006313 - transposition, DNA-mediated [Evidence IEA]
COORDINATES: protein motif:HMM:NF033516.0
GO:0004803 - transposase activity [Evidence IEA]
COORDINATES: protein motif:HMM:NF026982.2
restriction endonuclease fold toxin-2 domain-containing protein
COORDINATES: protein motif:HMM:NF015422.2
helicase associated domain-containing protein
COORDINATES: similar to AA sequence:RefSeq:WP_019075787.1
COORDINATES: protein motif:HMM:NF017760.2
GO:0022857 - transmembrane transporter activity [Evidence IEA]
GO:0055085 - transmembrane transport [Evidence IEA]
COORDINATES: protein motif:HMM:NF039924.1
COORDINATES: protein motif:HMM:NF025186.2
COORDINATES: similar to AA sequence:RefSeq:WP_007453328.1
COORDINATES: similar to AA sequence:RefSeq:WP_019056892.1
COORDINATES: protein motif:HMM:NF013828.2
COORDINATES: similar to AA sequence:RefSeq:WP_014139532.1
GO:0004521 - endoribonuclease activity [Evidence IEA]
GO:0043571 - maintenance of CRISPR repeat elements [Evidence IEA]
type I-E CRISPR-associated endoribonuclease Cas2e
COORDINATES: similar to AA sequence:RefSeq:WP_014139531.1
GO:0004520 - endodeoxyribonuclease activity [Evidence IEA]; GO:0046872 - metal ion binding [Evidence IEA]; GO:0003676 - nucleic acid binding [Evidence IEA]
GO:0051607 - defense response to virus [Evidence IEA]; GO:0043571 - maintenance of CRISPR repeat elements [Evidence IEA]
type I-E CRISPR-associated endonuclease Cas1e
COORDINATES: similar to AA sequence:RefSeq:WP_014139530.1
GO:0003674 - molecular_function [Evidence IEA]
type I-E CRISPR-associated protein Cas6/Cse3/CasE
COORDINATES: similar to AA sequence:RefSeq:WP_014139529.1
type I-E CRISPR-associated protein Cas5/CasD
COORDINATES: similar to AA sequence:RefSeq:WP_005477412.1
type I-E CRISPR-associated protein Cas7/Cse4/CasC
COORDINATES: protein motif:HMM:NF021034.2
type I-E CRISPR-associated protein Cse2/CasB
COORDINATES: similar to AA sequence:RefSeq:WP_020115033.1
type I-E CRISPR-associated protein Cse1/CasA
COORDINATES: similar to AA sequence:RefSeq:WP_019984167.1
CRISPR-associated helicase/endonuclease Cas3
COORDINATES: similar to AA sequence:RefSeq:WP_007268522.1
COORDINATES: similar to AA sequence:RefSeq:WP_004947001.1
COORDINATES: protein motif:HMM:NF017103.2
COORDINATES: similar to AA sequence:RefSeq:WP_006373850.1
GO:0008080 - N-acetyltransferase activity [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_018488969.1
COORDINATES: similar to AA sequence:RefSeq:WP_011039386.1
GO:0016491 - oxidoreductase activity [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_011039387.1
isochorismatase family cysteine hydrolase
COORDINATES: similar to AA sequence:RefSeq:WP_011039388.1
helix-turn-helix domain-containing protein
COORDINATES: similar to AA sequence:RefSeq:WP_019063039.1
COORDINATES: similar to AA sequence:RefSeq:WP_014138019.1
COORDINATES: similar to AA sequence:RefSeq:WP_018569250.1
COORDINATES: similar to AA sequence:RefSeq:WP_011109710.1
COORDINATES: protein motif:HMM:NF025052.2
COORDINATES: protein motif:HMM:NF015620.2
COORDINATES: similar to AA sequence:RefSeq:WP_007824764.1
COORDINATES: similar to AA sequence:RefSeq:WP_009152308.1
COORDINATES: similar to AA sequence:RefSeq:WP_014140679.1
COORDINATES: similar to AA sequence:RefSeq:WP_007387433.1
COORDINATES: protein motif:HMM:TIGR00180.1
GO:0007059 - chromosome segregation [Evidence IEA]; GO:0000910 - cytokinesis [Evidence IEA]
ParB/RepB/Spo0J family partition protein
COORDINATES: similar to AA sequence:RefSeq:WP_006545066.1
COORDINATES: similar to AA sequence:RefSeq:WP_016824002.1
COORDINATES: similar to AA sequence:RefSeq:WP_018829358.1
frameshifted; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: protein motif:HMM:NF040051.1
COORDINATES: similar to AA sequence:RefSeq:WP_007268197.1
COORDINATES: similar to AA sequence:RefSeq:WP_007495352.1
COORDINATES: similar to AA sequence:RefSeq:WP_014135508.1
GO:0016787 - hydrolase activity [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_014135507.1
GO:0003824 - catalytic activity [Evidence IEA]
fumarylacetoacetate hydrolase family protein
COORDINATES: similar to AA sequence:RefSeq:WP_014135506.1
COORDINATES: similar to AA sequence:RefSeq:WP_011039354.1
GO:0008701 - 4-hydroxy-2-oxovalerate aldolase activity [Evidence IEA]
incomplete; partial in the middle of a contig; missing C-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: protein motif:HMM:NF012759.2
COORDINATES: similar to AA sequence:RefSeq:WP_014135504.1
COORDINATES: similar to AA sequence:RefSeq:WP_014135503.1
COORDINATES: similar to AA sequence:RefSeq:WP_014135502.1
COORDINATES: similar to AA sequence:RefSeq:WP_011026834.1
programmed frameshift; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: similar to AA sequence:RefSeq:WP_006141891.1
COORDINATES: similar to AA sequence:RefSeq:WP_007463334.1
COORDINATES: similar to AA sequence:RefSeq:WP_011873634.1
COORDINATES: protein motif:HMM:NF024109.2
COORDINATES: similar to AA sequence:RefSeq:WP_020114957.1
COORDINATES: similar to AA sequence:RefSeq:WP_009311895.1
COORDINATES: similar to AA sequence:RefSeq:WP_019327906.1
GO:0045027 - DNA end binding [Evidence IEA]
GO:0006303 - double-strand break repair via nonhomologous end joining [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_019331065.1
COORDINATES: similar to AA sequence:RefSeq:WP_013735107.1
COORDINATES: similar to AA sequence:RefSeq:WP_020215754.1
COORDINATES: similar to AA sequence:RefSeq:WP_016572371.1
GO:0004252 - serine-type endopeptidase activity [Evidence IEA]
GO:0006282 - regulation of DNA repair [Evidence IEA]; GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]
COORDINATES: protein motif:HMM:TIGR02779.1
GO:0005524 - ATP binding [Evidence IEA]; GO:0003910 - DNA ligase (ATP) activity [Evidence IEA]
GO:0006310 - DNA recombination [Evidence IEA]; GO:0006281 - DNA repair [Evidence IEA]
COORDINATES: protein motif:HMM:NF024949.2
helix-turn-helix transcriptional regulator
COORDINATES: similar to AA sequence:RefSeq:WP_018588268.1
COORDINATES: similar to AA sequence:RefSeq:WP_014181271.1
COORDINATES: similar to AA sequence:RefSeq:WP_014151880.1
COORDINATES: similar to AA sequence:RefSeq:WP_014134516.1
COORDINATES: similar to AA sequence:RefSeq:WP_005477086.1
internal stop; incomplete; partial in the middle of a contig; missing N-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: similar to AA sequence:RefSeq:WP_018801738.1
incomplete; partial in the middle of a contig; missing N-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology.
nucleotidyltransferase domain-containing protein
COORDINATES: similar to AA sequence:RefSeq:WP_012998046.1
COORDINATES: protein motif:HMM:NF016334.2
ankyrin repeat domain-containing protein
COORDINATES: similar to AA sequence:RefSeq:WP_011435553.1
COORDINATES: similar to AA sequence:RefSeq:WP_006379839.1
COORDINATES: similar to AA sequence:RefSeq:WP_020139230.1
frameshifted; incomplete; partial in the middle of a contig; missing N-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: similar to AA sequence:RefSeq:WP_007267742.1
COORDINATES: protein motif:HMM:NF040047.1
COORDINATES: similar to AA sequence:RefSeq:WP_006374533.1
COORDINATES: similar to AA sequence:RefSeq:WP_006376342.1
COORDINATES: similar to AA sequence:RefSeq:WP_009310241.1
COORDINATES: similar to AA sequence:RefSeq:WP_020217043.1
GO:0003964 - RNA-directed DNA polymerase activity [Evidence IEA]
GO:0000373 - Group II intron splicing [Evidence IEA]
group II intron reverse transcriptase/maturase
COORDINATES: similar to AA sequence:RefSeq:WP_014677534.1
COORDINATES: similar to AA sequence:RefSeq:WP_015613140.1
frameshifted; incomplete; partial in the middle of a contig; missing C-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: protein motif:HMM:NF025030.2
COORDINATES: similar to AA sequence:RefSeq:WP_020631530.1
COORDINATES: similar to AA sequence:RefSeq:WP_019929407.1
COORDINATES: similar to AA sequence:RefSeq:WP_003952956.1
incomplete; partial in the middle of a contig; missing N-terminus and C-terminus; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: similar to AA sequence:RefSeq:WP_005452654.1
COORDINATES: similar to AA sequence:RefSeq:WP_014178341.1
COORDINATES: similar to AA sequence:RefSeq:WP_005452651.1
COORDINATES: similar to AA sequence:RefSeq:WP_009720961.1
COORDINATES: similar to AA sequence:RefSeq:WP_007073636.1
COORDINATES: similar to AA sequence:RefSeq:WP_018735751.1
COORDINATES: protein motif:HMM:NF017984.2
GO:0019104 - DNA N-glycosylase activity [Evidence IEA]
GO:0036297 - interstrand cross-link repair [Evidence IEA]
crosslink repair DNA glycosylase YcaQ family protein
COORDINATES: similar to AA sequence:RefSeq:WP_018812757.1
COORDINATES: similar to AA sequence:RefSeq:WP_076611777.1
COORDINATES: protein motif:HMM:NF024734.2
type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein
COORDINATES: protein motif:HMM:NF015638.2
COORDINATES: similar to AA sequence:RefSeq:WP_009717347.1
COORDINATES: similar to AA sequence:RefSeq:WP_014061116.1
COORDINATES: similar to AA sequence:RefSeq:WP_009717345.1
COORDINATES: similar to AA sequence:RefSeq:WP_014056015.1
COORDINATES: similar to AA sequence:RefSeq:WP_009327053.1
methyltransferase domain-containing protein
COORDINATES: protein motif:HMM:NF040316.1
COORDINATES: similar to AA sequence:RefSeq:WP_010471577.1
COORDINATES: similar to AA sequence:RefSeq:WP_006139001.1
COORDINATES: similar to AA sequence:RefSeq:WP_006139000.1
COORDINATES: protein motif:HMM:NF016724.2
COORDINATES: protein motif:HMM:NF012406.2
COORDINATES: similar to AA sequence:RefSeq:WP_076611121.1
COORDINATES: protein motif:HMM:NF020622.2
COORDINATES: similar to AA sequence:RefSeq:WP_086558126.1
COORDINATES: similar to AA sequence:RefSeq:WP_016828249.1
COORDINATES: similar to AA sequence:RefSeq:WP_011779742.1
COORDINATES: similar to AA sequence:RefSeq:WP_020576260.1
COORDINATES: similar to AA sequence:RefSeq:WP_014140039.1
COORDINATES: similar to AA sequence:RefSeq:WP_005477250.1
COORDINATES: similar to AA sequence:RefSeq:WP_005477251.1
TnsA-like heteromeric transposase endonuclease subunit
COORDINATES: similar to AA sequence:RefSeq:WP_005477252.1
GO:0015074 - DNA integration [Evidence IEA]
DDE-type integrase/transposase/recombinase
COORDINATES: similar to AA sequence:RefSeq:WP_020550093.1