Trypanosoma brucei brucei TREU927 chromosome 10, whole genome shotgun sequence.
Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Trypanosoma.
The genome of the African trypanosome Trypanosoma brucei
Comparative genomics of trypanosomatid parasitic protozoa
Submitted (10-APR-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (14-APR-2005) Wellcome Trust Genome Campus, The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK
PROVISIONAL REFSEQ: This record has not yet been subject to final\nNCBI review. The reference sequence is identical to CM000208.\nTrypanosoma brucei chromosomes 9 to 11 were isolated and then\nsequenced by the Wellcome Trust Sanger Institute, using a whole\nchromosome shotgun approach. The sequences are therefore, likely to\nrepresent a 'mosaic' assembly of both chromosomal homologs.\nDetails of Trypanosoma sequencing at the Sanger Institute are\navailable on the World Wide Web. (URL,\nhttp://www.sanger.ac.uk/Projects/T_brucei/) and via the GeneDB\ndatabase (www.genedb.org).\nPredicted protein coding regions (>30 codons) were manually revised\nin the light of similarity data, domain information and orthology\nto Leishmania major and Trypanosoma cruzi. Gene products were\nassigned putative functions based on similarity with experimentally\ncharacterized gene products or the presence of predicted protein\ndomains. Products were labelled either 'hypothetical protein', or\n'hypothetical protein, conserved' when showing significant\nsimilarity with proteins or domains of unknown function from other\norganisms. Short predicted coding sequences (less than 150 codons)\nwith no additional evidence of coding potential were labeled\n'hypothetical protein, unlikely'.\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: INSDC submitter\nAnnotation Status :: Full annotation\nAnnotation Pipeline :: Eukaryotic Annotation Propagation Pipeline\n##Genome-Annotation-Data-END##
single VAT derivative of TREU (Trypanosomiasis Research Edinburgh University) 927/4 (GPAL/KE/70/EATRO 1534)
expression site-associated gene ESAG protein
variant surface glycoprotein (VSG, pseudogene), putative
expression site-associated gene (ESAG, pseudogene), putative
retrotransposon hot spot protein (RHS, pseudogene), putative
cytochrome C oxidase subunit VI, putative
mitochondrial structure specific endonuclease I (SSE-1), putative
curated by M. Parsons, P. Ward, J. Mottram; GB:AAA19805.3: dual-specificity tyrosine-(Y)- phosphorylation regulated kinase TbPK4 {Trypanosoma brucei;}
similar to SP:Q96PY6: Serine/threonine-protein kinase Nek1 (EC 2.7.1.37) (NimA-relatedprotein kinase 1) (NY-REN-55 antigen). {Homo sapiens}; curated by M. Parsons, P. Ward, J. Mottram
ATP-dependent DEAD/H RNA helicase, putative
similar to GB:CAB95532.1: 40S ribosomal protein S11, probable {Trypanosoma brucei}; similar to 40S ribosomal protein S11. (Swiss-Prot:P42756) (Dunaliella tertiolecta)
2-oxoisovalerate dehydrogenase subunit alpha
structural maintenance of chromosome 4, putative
similar to Structural maintenance of chromosome 4. (Swiss-Prot:Q12267) (Saccharomyces cerevisiae); similar to GB:AAS44544.1: structural maintenance of chromosome protein 4 {Trypanosoma cruzi); similar to SP:Q8CG47: Structural maintenance of chro
vesicle-associated membrane protein, putative
Curated by Mark Field. similar to Vesicle- associated membrane protein 722 (AtVAMP722) (Synaptobrevin- related protein 1). (Swiss-Prot:P47192) (Arabidopsis thaliana;)
ungrouped kinesin (curated by B. Wickstead, Univ. of Oxford)
DNA repair and recombination helicase protein PIF1, putative
Curated by P. Englund. similar to DNA repair and recombination protein pif1, mitochondrial precursor. (Swiss-Prot:Q9UUA2) (Schizosaccharomyces pombe); similar to DNA repair and recombination protein PIF1, mitochondrial precursor. (Swiss-Prot:P0727
GPI-Anchor Signal predicted for Tb10.70.7150 by DGPI v2.04 with cleavage site probability 0.24939999 near 361; similar to TolA protein. (Swiss-Prot:P19934) (Escherichia coli;)
serine carboxypeptidase III precursor, putative
Curated by J. Mottram. similar to Serine carboxypeptidase III precursor (EC 3.4.16.5) (CP-MIII). (Swiss-Prot:P21529) (Hordeum vulgare)
Curated by J. Mottram. GB:AAO74600.1: serine carboxypeptidase precursor {Trypanosoma cruzi;}
Curated by J. Mottram. similar to Serine carboxypeptidase III precursor (EC 3.4.16.5). (Swiss- Prot:32826)(Arabidopsis thaliana)
similar to T-complex protein 1, delta subunit (TCP-1-delta) (CCT-delta). (Swiss-Prot:P47208) (Caenorhabditis elegans;)
cell division protein kinase 2 homolog 1
curated by M. Parsons, P. Ward, J. Mottram; identical to SP:P38973: Cell division control protein 2 homolog 1 (EC 2.7.1.-). {Trypanosoma brucei brucei;}
similar to 40S ribosomal protein S23. (Swiss- Prot:P39028) (Homo sapiens;Mus musculus;Rattus norvegicus)
similar to 33 kDa inner dynein arm light chain, axonemal (p33). (Swiss-Prot:Q26630) (Strongylocentrotus purpuratus;)
identical to GB:AAF32302.2: epsilon tubulin {Trypanosoma brucei}; similar to Tubulin epsilon chain (Epsilon tubulin). (Swiss- Prot:Q9UJT0) (Homo sapiens); similar to Tubulin beta-1 chain. (Swiss-Prot:P18241) (Brugia pahangi;)
katanin p60-like protein 2 (curated by B. Wickstead, Univ. of Oxford)
serine/threonine-protein kinase, putative
hypoxanthine-guanine phosphoribosyltransferase, putative
hypoxanthine-guanine phosphoribosyltransferase
similar to SP:Q8VDF3: Death-associated protein kinase 2 (EC 2.7.1.37) (DAP kinase 2) (DAP-kinase related protein 1) (DRP-1). {Mus musculus}; similar to SP:Q9ES74: Serine/threonine-protein kinase Nek7 (EC 2.7.1.37) (NimA-relatedprotein kinase 7). {
similar to Methionyl-tRNA synthetase, mitochondrial (EC 6.1.1.10) (Methionine--tRNA ligase) (MetRS). (Swiss-Prot:O74634) (Candida albicans;)
similar to SP:P55745: Ras-related protein Rab- 21. {Canis familiaris}
proteasome regulatory non-ATP-ase subunit 5
similar to ATP synthase lipid-binding protein, mitochondrial precursor(EC 3.6.3.14) (ATP synthase proteolipid P2) (ATPase protein 9) (ATPase subunit C). (Swiss-Prot:Q06055) (Homo sapiens)
dual specificity protein phosphatase, putative
identical to GB:AAL86696.1: histone deacetylase-like protein HDO1 {Trypanosoma brucei;} (PMID:12100550)
similar to Nucleotide-binding protein 1 (NBP 1). (Swiss-Prot:Q9R060) (Mus musculus;)
weak similarity to syntaxin and t-SNARE proteins Curated by Mark Field.
contains one TmHMM; similar to Protein kinase byr2 (EC 2.7.1.-) (Protein kinase ste8) (MAPK kinasekinase) (MAPKKK). (Swiss-Prot:P28829) (Schizosaccharomyces pombe) curated by M. Parsons, P. Ward, J. Mottram
similar to GB:CAC14329.1: major vault protein {Leishmania major}; similar to Major vault protein alpha (MVP-alpha) (Swiss-Prot:P34118) (Dictyostelium discoideum); similar to Major vault protein (MVP) (Lung resistance- related protein). (Swiss-Prot
member of ARP6 family (curated by B. Wickstead, Univ. of Oxford)
curated by M. Parsons, P. Ward, J. Mottram
identical to SP:P41166: Elongation factor 1- alpha (EF-1-alpha). {Trypanosoma brucei brucei}
identical to SP:P41166: Elongation factor 1- alpha (EF-1-alpha). {Trypanosoma brucei brucei;}
most (but not all) Tryp predicetd proteins have a substantial N-yerminal extension (of 100aa)
mitochondrial processing peptidase alpha subunit (pseudogene), putative
ubiquitin carboxyl-terminal hydrolase, putative
ubiquitin-conjugating enzyme variant Kua homologue, putative
vacuolar protein sorting-associated protein 35, putative
similar to SP:Q9QYJ0: DnaJ homolog subfamily A member 2 (mDj3). {Mus musculus;}
dihydrolipoamide acetyltransferase, putative
similar to La protein homolog (La ribonucleoprotein) (La autoantigen homolog). (Swiss- Prot:P40796) (Drosophila melanogaster)
identical to:GB:AAO72980.1: major surface protease-like protein C {Trypanosoma brucei;} PMID:12707278
receptor-type adenylate cyclase GRESAG 4, putative
weak similarity to chaperone protein DNAJ
greatest similarity to plant glyoxysomal MDH
mitochondrial; identical to GB:AAC27101.1: malate dehydrogenase {Trypanosoma brucei;}
lysosomal alpha-mannosidase precursor, putative
contains WD40 repeats, similar to U3 small nucleolar RNA interacting protein 2
eukaryotic translation initiation factor 5, putative
DNA-directed RNA polymerase III largest subunit
GPI-Anchor Signal predicted for Tb10.70.4830 by DGPI v2.04 with cleavage site probability 0.702 near 476
splicing factor PTSR1 interacting protein
GPI-Anchor Signal predicted for Tb10.70.4600 by DGPI v2.04, no cleavage site predicted
proteasome regulatory non-ATPase subunit 11
Curated by J. Mottram. identical to GB:AAL72634.1: proteasome regulatory non-ATP-ase subunit 11 {Trypanosoma brucei}; similar to 26S proteasome regulatory subunit rpn11. (Swiss-Prot:P41878) (Schizosaccharomyces pombe)
Curated by J. Mottram; identical to GB:AAL72634.1: proteasome regulatory non-ATP-ase subunit 11 {Trypanosoma brucei}; similar to 26S proteasome regulatory subunit rpn11. (Swiss-Prot:P41878) (Schizosaccharomyces pombe)
glycerol-3-phosphate acyltransferase, putative
cytochrome C oxidase assembly protein, putative
delta-1-pyrroline-5-carboxylate dehydrogenase, putative
similar to Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial precursor(EC 1.5.1.12) (P5C dehydrogenase). (Swiss-Prot:P30038) (Homo sapiens)
similar to Long-chain-fatty-acid--CoA ligase 4 (EC 6.2.1.3) (Long-chain acyl-CoAsynthetase 4) (LACS 4). (Swiss-Prot:O35547) (Rattus norvegicus); similar to GB:AAM68991.1: long chain fatty acyl CoA synthetase 6 {Leishmania major}
curated by M. Parsons, P. Ward, J. Mottram; probably only fragment as end of contig; similar to GB:AAM69001.1: serine-threonine protein kinase 2, putative {Leishmania major}
60S acidic ribosomal protein P2, putative
GPI-Anchor Signal predicted for Tb10.70.4020 by DGPI v2.04 with cleavage site probability 0.408 near 77
Curated by J. Mottram; identical to GB:AAF91246.1: proteasome regulatory ATPase subunit 4 {Trypanosoma brucei}
GB:AAO63566.1: RNA editing complex protein MP99 {Trypanosoma brucei;} PMID:12649499
GPI-Anchor Signal predicted for Tb10.70.3780 by DGPI v2.04 with cleavage site probability 0.684 near 286
NADH-dependent fumarate reductase, putative
identical to GB:AAK73815.1: aspartate aminotransferase {Trypanosoma brucei;} PMID:11443076
vacuolar ATP synthase subunit D, putative
TcBr homologs are much shorter at C terminus
phosphatidylinositol-4-phosphate 5-kinase, putative
similar to Probable phosphatidylinositol-4- phosphate 5-kinase MSS4 (EC 2.7.1.68)(1- phosphatidylinositol-4-phosphate kinase) (PIP5K) (PtdIns(4)P-5-kinase) (Diphosphoinositide kinase). (Swiss- Prot:P38994) (Saccharomyces cerevisiae;)
ATP-dependent DEAD-box RNA helicase, putative
methylglutaconyl-CoA hydratase mitochondrial precursor
similar to Methylglutaconyl-CoA hydratase, mitochondrial precursor (EC 4.1.2.18)(AU-specific RNA- binding enoyl-CoA hydratase) (AU-binding enoyl- CoAhydratase) (muAUH). (Swiss-Prot:Q9JLZ3) (Mus musculus)
GPI-Anchor Signal predicted for Tb10.70.3270 by DGPI v2.04 with cleavage site probability 0.702 near 653
similar to Serine palmitoyltransferase 2 (EC 2.3.1.50) (Long chain basebiosynthesis protein 2) (LCB 2) (Serine-palmitoyl-CoA transferase 2)(SPT 2). (Swiss- Prot:O15270) (Homo sapiens)
GPI-Anchor Signal predicted for Tb10.70.3125 by DGPI v2.04, no cleavage site predicted
2-oxoisovalerate dehydrogenase subunit beta
similar to 2-oxoisovalerate dehydrogenase beta subunit, mitochondrial precursor(EC 1.2.4.4) (Branched- chain alpha-keto acid dehydrogenase E1component beta chain) (BCKDH E1-beta). (Swiss-Prot:P21839) (Bos taurus;)
GPI-Anchor Signal predicted for Tb10.70.2850 by DGPI v2.04 with cleavage site probability 0.51600003 near 231
GPI-Anchor Signal predicted for Tb10.70.2840 by DGPI v2.04 with cleavage site probability 0.51600003 near 224
GPI-Anchor Signal predicted for Tb10.70.2820 by DGPI v2.04, no cleavage site predicted
identical to:GB:AAK62674.1: PUF1 {Trypanosoma brucei;} PMID:11897132
stress-inducible protein STI1-like, putative
leucine carboxyl methyltransferase, putative
GPI-Anchor Signal predicted for Tb10.70.2750 by DGPI v2.04, no cleavage site predicted
mRNA capping methyltransferase, putative
similar to 6-phosphofructo-2-kinase/fructose- 2,6-biphosphatase 4 (6PF-2-K/Fru-2,6-P2ASE testis-type isozyme). (Swiss-Prot:Q16877) (Homo sapiens)
ARP2/3 complex subunit 3 (curated by B. Wickstead, Univ. of Oxford)
mitochondrial inner membrane signal peptidase, putative
dolichyl-phosphate beta-D-mannosyltransferase precursor, putative
peptidyl-prolyl cis-trans isomerase, putative
similar to FK506-binding protein 1 (FKBP) (Peptidyl-prolyl cis-trans isomerase)(PPIase) (EC 5.2.1.8) (Rapamycin-binding protein). (Swiss-Prot:P20081) (Saccharomyces cerevisiae)
similar to Phosphatidylinositol-4-phosphate 5- kinase type II alpha (EC 2.7.1.149)(PIP5KII-alpha) (1- phosphatidylinositol-4-phosphate 5-kinase)(PtdIns(4)P-5- kinase B isoform) (Diphosphoinositide kinase). (Swiss- Prot:O70172) (Mus musculus;)
tRNA-dihydrouridine synthase 2, putative
similar to tRNA-dihydrouridine synthase 2 (EC 1.-.-.-) (Swiss-Prot:P53720) (Saccharomyces cerevisiae)
similar to DnaJ homolog subfamily C member 7 (Tetratricopeptide repeat protein 2)(TPR repeat protein 2) (Swiss-Prot:Q99615) (Homo sapiens)
small nuclear ribonucleoprotein, putative
similar to Ubiquitin-like protein DSK2. (Swiss- Prot:P48510) (Saccharomyces cerevisiae;)
curated by M. Parsons, P. Ward, J. Mottram; similar to SP:P54666: Cell division control protein 2 homolog 3 (EC 2.7.1.-). {Trypanosoma brucei brucei;}
ubiquitin/ribosomal protein S27a, putative
GPI-Anchor Signal predicted for Tb10.70.2100 by DGPI v2.04 with cleavage site probability 1.72 near 189
TPR-repeat-containing chaperone protein DnaJ
similar over 419aa to SP:Q99615: DnaJ homolog subfamily C member 7 (Tetratricopeptide repeat protein 2)(TPR repeat protein 2). {Homo sapiens;}
contains probable cyclic nucleotide-binding domain
pre-mRNA splicing factor ATP-dependent RNA helicase, putative
similar to ATP-dependent helicase DHX8 (RNA helicase HRH1) (DEAH-box protein 8) (Swiss-Prot:Q14562) (Homo sapiens)
similar to HIRA-interacting protein 5 (mHIRIP5) (Swiss-Prot:Q9QZ23) (Mus musculus)
eukaryotic translation initiation factor 6
similar to 40S ribosomal protein S18. (Swiss- Prot:P34788) (Arabidopsis thaliana)
similar to 60S ribosomal protein L24 (L30) (Swiss-Prot:P83732) (Rattus norvegicus)
endonuclease/exonuclease/phosphatase, putative
GPI-Anchor Signal predicted for Tb10.70.1510 by DGPI v2.04, no cleavage site predicted possible inositol polyphosphate-5-phosphatase
similar to Protein phosphatase 2C (EC 3.1.3.16) (PP2C) (Swiss-Prot:P49444) (Paramecium tetraurelia); similar to Protein phosphatase 2C (EC 3.1.3.16) (PP2C). (Swiss-Prot:P36982) (Leishmania donovani chagasi)
fructose-bisphosphate aldolase glycosomal
contains DNAJ domain; similar to GB:AAC63051.1: heat shock protein {Trypanosoma cruzi;}
N-acetyltransferase subunit Nat1, putative
GPI-Anchor Signal predicted for Tb10.70.1310 by DGPI v2.04, no cleavage site predicted; similar to SP:Q01889: PAG1 protein precursor. {Trypanosoma brucei brucei;}
similar to GB:AAG59622.1: procyclin-associated gene 2 polypeptide {Trypanosoma brucei;}
GPI-Anchor Signal predicted for Tb10.70.1290 by DGPI v2.04 with cleavage site probability 0.344 near 210
GPI-Anchor Signal predicted for Tb10.70.1280 by DGPI v2.04 with cleavage site probability 0.344 near 206
identical to GB:AAC02215: valosin-containing protein homolog (Trypanosoma brucei)
GPI-Anchor Signal predicted for Tb10.70.1170 by DGPI v2.04, no cleavage site predicted
contains Zn-finger in Ran binding domain
translation elongation factor 1-beta, putative
universal minicircle sequence binding protein (UMSBP), putative
similar to DNA-binding protein HEXBP (Hexamer- binding protein). (Swiss-Prot:Q04832) (Leishmania major;)
curated by J. Mottram curated by J. Mottram
phosphatidylinositol N-acetylglucosaminyltransferase subunit c
similar to Succinate semialdehyde dehydrogenase (EC 1.2.1.24) (NAD(+)-dependentsuccinic semialdehyde dehydrogenase). (Swiss-Prot:P51650) (Rattus norvegicus)
check underlying sequence - possible longer orf
XRND: pers. comm. C. Clayton, University of Heidelberg; similar to Protein dhp1 (Swiss- Prot:P40848) (Schizosaccharomyces pombe)
similar to GB:AAH08065.1: Di-Ras2 {Homo sapiens}
similar to Major vault protein beta (MVP-beta). (Swiss-Prot:P54659) (Dictyostelium discoideum); similar to Major vault protein (MVP) (Lung resistance- related protein). (Swiss-Prot:Q14764) (Homo sapiens)
serine/threonine protein phosphatase 2b catalytic subunit A2, putative
GPI-Anchor Signal predicted for Tb10.70.0330 by DGPI v2.04 with cleavage site probability 0.74819994 near 163
serine/threonine protein phosphatase, putative
ATP-dependent DEAD/H RNA helicase HEL64, putative
identical to Putative DEAD-box RNA helicase HEL64. (Swiss-Prot:Q26696) (Trypanosoma brucei brucei); similar to P68-like protein. (Swiss-Prot:P24782) (Schizosaccharomyces pombe;)
COP-coated vesicle membrane protein Erv25 precursor
similar to Dynein light chain (Swiss- Prot:Q94758) (Schistosoma mansoni) and to Dynein light chain 1, cytoplasmic. (Swiss-Prot:Q02647) (Saccharomyces cerevisiae;)
tyrosine specific protein phosphatase, putative
vacuolar protein sorting-associated protein 45, putative
similar to Vacuolar protein sorting-associated protein 45 (rvps45). (Swiss-Prot:O08700) (Rattus norvegicus); similar to Vacuolar protein-sorting protein 45 homolog (AtVPS45). (Swiss-Prot:O49048) (Arabidopsis thaliana;)
developmentally regulated GTP-binding protein, putative
glyceraldehyde 3-phosphate dehydrogenase cytosolic
identical to SP:P10097: Glyceraldehyde 3- phosphate dehydrogenase, cytosolic (GAPDH). {Trypanosoma brucei brucei;}
dipeptidyl-peptidase 8-like serine peptidase
chromatin assembly factor 1 subunit B, putative
myo-inositol-1-phosphate synthase, putative
similar to Lysosomal acid phosphatase precursor (EC 3.1.3.2) (LAP). (Swiss-Prot:P11117) (Homo sapiens)
identical to SP:Q01889: PAG1 protein precursor {Trypanosoma brucei brucei}
GPI-Anchor Signal predicted for Tb10.6k15.3520 by DGPI v2.04 with cleavage site probability 0.14400001 near 89
cysteine-rich acidic integral membrane protein precursor
identical/ similar to SP:Q03650: Cysteine-rich, acidic integral membrane protein precursor. {Trypanosoma brucei brucei}
terminal uridylyltransferase 3, putative
succinyl-CoA ligase [GDP-forming] beta-chain, putative
similar to Exosome complex exonuclease RRP41 (EC 3.1.13.-) (Ribosomal RNAprocessing protein 41) (p12A). (Swiss-Prot:Q9NPD3) (Homo sapiens;) similar to GB:CAC39258.1: Rrp41p homologue {Trypanosoma brucei;}
similar to SP:Q8ITY4: Pre-mRNA branch site p14-like protein. {Caenorhabditis elegans;} similar to Pre-mRNA branch site protein p14 (CGI-110) (HSPC175) (Ht006). (Swiss-Prot:Q9Y3B4) (Homo sapiens;)
GPI-Anchor Signal predicted for Tb10.6k15.3180 by DGPI v2.04 with cleavage site probability 3.7410002 near 177
similar to Cullin homolog 4B (CUL-4B). (Swiss- Prot:Q13620) (Homo sapiens;)
similar to HECT domain and RCC1-like domain protein 3. (Swiss-Prot:Q15034) (Homo sapiens;)
folylpolyglutamate-dihydrofolate synthetase, putative
dihydrolipoamide acetyltransferase precursor, putative
mitochondrial ATP-dependent zinc metallopeptidase, putative
similar to TBC1 domain family member 8 (Vascular Rab-GAP/TBC-containing protein)(AD 3) (Swiss-Prot:O95759) (Homo sapiens) and (Swiss-Prot:Q9Z1A9) (Mus musculus)
protein transport protein Sec23A, putative
ubiquitin fusion degradation protein, putative
COP9 signalosome complex subunit, putative
similar over 201aa to GB:AAF60803.1: Cop-9 signalosome subunit protein 4 {Caenorhabditis elegans;}
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
weakly similar to WD-repeat protein 9. (Swiss- Prot:Q9NSI6) (Homo sapiens)
similar to T-complex protein 1, theta subunit (TCP-1-theta) (CCT-theta). (Swiss-Prot:P50990) (Homo sapiens)
GPI-Anchor Signal predicted for Tb10.6k15.2300 by DGPI v2.04, no cleavage site predicted
eukaryotic translation initiation factor 3 subunit 8, putative
similar to GB:CAA06683.1: poly(A)-specific ribonuclease {Homo sapiens;}
similar to SP:Q06221: Glucose transporter 1B/1C/1D/1F/2B (Trypanosoma brucei); GB:AAA92489.1: hexose transporter {Trypanosoma brucei;}
similar to PIR:T09134: GB|AAC32772.1|GP|3452213|AF031926 GTP-binding protein homolog RAB1 - Trypanosoma brucei {Trypanosoma brucei;}
similarity to ferrodoxin-like sucrose cleavage protein
possible ubiquinone biosynthesis protein; possible ABC1 kinase
similarity to putative plant adhesion molecules; similar to TBC1 domain family member 1 (Swiss- Prot:Q86TI0) (Homo sapiens)
similarity to putative plant adhesion molecules
kinetoplast DNA-associated protein, putative
ethanolaminephosphotransferase, putative
GPI-Anchor Signal predicted for Tb10.6k15.1510 by DGPI v2.04 with cleavage site probability 0.172 near 686
possible eukaryotic translation initiation factor 3 gamma subunit
similar to SP:Q14562: ATP-dependent helicase DHX8 (RNA helicase HRH1) (DEAH-box protein 8). {Homo sapiens}
similar to Isoleucyl-tRNA synthetase, cytoplasmic (EC 6.1.1.5) (Isoleucine--tRNAligase) (IleRS) (IRS). (Swiss-Prot:P41252) (Homo sapiens;)
(curated by B. Wickstead, Univ. of Oxford)
GPI-Anchor Signal predicted for Tb10.6k15.1110 by DGPI v2.04 with cleavage site probability 0.48000002 near 336
similar to Adiponectin receptor protein 1. (Swiss-Prot:Q91VH1) (Mus musculus)
phosphoribosylpyrophosphate synthetase, putative
similar to Ribose-phosphate pyrophosphokinase (EC 2.7.6.1) (RPPK) (Phosphoribosylpyrophosphate synthetase) (P-Rib-PP synthetase) (PRPP synthetase). (Swiss-Prot:P42816) (Bacillus caldolyticus)
expression site-associated gene 3 (ESAG3)-like protein
similar to GB:AAD31319.2: Mx-interacting protein kinase PKM {Mesocricetus auratus}
identical to GB:AAF91248.1: proteasome regulatory ATPase subunit 6 {Trypanosoma brucei}
similar to Thyroid receptor interacting protein 12 (TRIP12). (Swiss-Prot:Q14669) (Homo sapiens); similar to Ubiquitin fusion degradation protein 4 (UB fusion protein 4). (Swiss-Prot:P33202) (Saccharomyces cerevisiae)
gamma-tubulin complex subunit 2 (curated by B. Wickstead, Univ. of Oxford); similar to Gamma-tubulin complex component 2 (GCP-2) (Spindle pole body proteinSpc97 homolog) (hSpc97) (hGCP2) (h103p) (Swiss- Prot:Q9BSJ2) (Homo sapiens)
mitochondrial intermediate peptidase, putative
procyclin-associated gene 4 (PAG4) polypeptide
GPI-Anchor Signal predicted for Tb10.6k15.0070 by DGPI v2.04 with cleavage site probability 1.131 near 356
similar to PAG1 protein precursor. (Swiss- Prot:Q01889) (Trypanosoma brucei brucei)
GPI-Anchor Signal predicted for Tb10.6k15.0040 by DGPI v2.04, no cleavage site predicted
GPI-Anchor Signal predicted for Tb10.6k15.0030 by DGPI v2.04 with cleavage site probability 0.64800006 near 107
GPI-Anchor Signal predicted for Tb10.6k15.0020 by DGPI v2.04 with cleavage site probability 0.64800006 near 119; identical to Procyclic form specific polypeptide B1-alpha precursor (Procyclin B1-alpha) (PARP). (Swiss- Prot:P08469) (Trypanosoma brucei
microtubule-associated protein, putative
some similarity to L major hypothetical, and in syntenic location.
structural maintenance of chromosome 2, putative
similar to Structural maintenance of chromosome 2 (Chromosome scaffold proteinScII). (Swiss-Prot:Q90988) (Gallus gallus); similar to Structural maintenance of chromosome 2 (Chromosome-associated proteinE) (Chromosome assembly protein XCAP-E). (Swi
high similarity to L major possible ribosomal protein, but little similarity to other spp = labelled conserved hypothetical wrt Lm
minichromosome maintenance complex subunit
PFAM search identifies MCM (minichromosome maintenance) domain and zinc finger; similar to DNA replication licensing factor MCM8 (Minichromosome maintenance 8). (Swiss-Prot:Q9UJA3) (Homo sapiens;)
ARP2/3 complex subunit 5 (curated by B. Wickstead, Univ. of Oxford)
low-ish similarity; but syntenic with CDS 3' to this in L major also
based on PFAM hit, clustering with hypothetical genes from multiple species
similar to Dual specificity protein phosphatase 10 (EC 3.1.3.48) (EC 3.1.3.16)(Mitogen-activated protein kinase phosphatase 5) (MAP kinasephosphatase 5) (MKP-5). (Swiss-Prot:Q9Y6W6) (Homo sapiens;)
match to Lm hypothetical CDS that is also adjacent to dual specificity phosphatase-like CDS
gene in T cruzi experimentally characterised
low similarity to zinc finger domain and cDNAs from macaque and human Curated by J. Mottram.
similarity to cDNA sequence from T cruzi, shown to have domain similarity to other adenosine deaminase genes; SMART/PFAM searches reveal relatively low similarity scores to adenosine deaminases from multiple species
Glycine-rich, supporting evidence from SMART and PFAM, similar to Glycine-rich cell wall structural protein precursor. (Swiss-Prot:P27483) (Arabidopsis thaliana;)
deoxyribodipyrimidine photolyase, putative
ubiquitin activating E1 enzyme, putative
similar to Autophagy protein APG7 (APG12 activating enzyme) (Swiss-Prot:P38862) (Saccharomyces cerevisiae)
GPI-Anchor Signal predicted for Tb10.26.0690 by DGPI v2.04 with cleavage site probability 1.026 near 330
protein phosphatase 2a regulatory subunit, putative
similar to 40S ribosomal protein S3. (Swiss- Prot:Q06559) (Drosophila melanogaster)
nicotinate phosphoribosyltransferase, putative
33 kDa inner dynein arm light chain axonemal
NAD or NADP dependent oxidoreductase, putative
similar to GB:AAK26163.1: kynurenine aminotransferase III {Homo sapiens}
similar to Syntaxin 41 (AtSYP41) (AtTLG2a). (Swiss-Prot:O65359) (Arabidopsis thaliana)
GPI-Anchor Signal predicted for Tb10.389.1690 by DGPI v2.04 with cleavage site probability 1.5480001 near 1464
ribulose-5-phosphate 3-epimerase, putative
cytosolic nonspecific dipeptidase, putative
weak similarity to Cytosolic nonspecific dipeptidase (Glutamate carboxypeptidase-likeprotein 1). (Swiss-Prot:Q96KP4) (Homo sapiens;)
UDP-Gal/UDP-GlcNAc-dependent glycosyltransferase
GPI-Anchor Signal predicted for Tb10.389.1220 by DGPI v2.04, no cleavage site predicted
LIH: member fo a family containing a previously- unrecognized domain.
pyruvate dehydrogenase E1 component subunit alpha
cholinephosphate cytidylyltransferase A, putative
3' 5'-cyclic nucleotide phosphodiesterase
similar to Calcium/calmodulin-dependent 3',5'- cyclic nucleotide phosphodiesterase 1C (Swiss-Prot: Q63421) (Rattus norvegicus)
vacuolar protein sorting complex subunit, putative
calpain-like cysteine peptidase, putative
similar to Dynein light chain 2B, cytoplasmic. (Swiss-Prot:Q8TF09) (Homo sapiens;)
UTP-glucose-1-phosphate uridylyltransferase 2, putative
GPI-Anchor Signal predicted for Tb10.389.0300 by DGPI v2.04, no cleavage site predicted
actin related protein 2/3 complex, putative
ARP2/3 complex subunit 1 (curated by B. Wickstead, Univ. of Oxford)
cyclophilin type peptidyl-prolyl cis-trans isomerase, putative
similar to Peptidyl-prolyl cis-trans isomerase ppi1 (EC 5.2.1.8) (Cyclophilinppi1). (Swiss-Prot:P87051) (Schizosaccharomyces pombe)
similar to 30S ribosomal protein S8. (Swiss- Prot:Q8G086) (Brucella melitensis biovar Suis;)
DNA-directed RNA polymerase II, putative
DNA-directed RNA polymerases II subunit, putative
similar to Cytoplasmic dynein light chain (T- complex testis-specific protein 1homolog) (Protein CW-1). (Swiss-Prot:Q15763) (Homo sapiens;Bos taurus;)
diphosphomevalonate decarboxylase, putative
serine/threonine protein phosphatase type 5
GPI-Anchor Signal predicted for Tb10.389.0030 by DGPI v2.04, no cleavage site predicted
phosphatidic acid phosphatase alpha, putative
calcium-dependent lipid binding protein, putative
similar to Synaptotagmin IV (SytIV). (Swiss- Prot:P50232) (Rattus norvegicus)
GPI-anchor transamidase subunit 8 (GPI8)
tubulin tyrosine ligase protein, putative
identical to GB:AAF29446.1: aconitase {Trypanosoma brucei brucei;} (PMID:10644738)
vacuolar ATP synthase subunit c, putative
GPI-Anchor Signal predicted for Tb10.61.2830 by DGPI v2.04, no cleavage site predicted
protein transport protein Sec13, putative
similar to Adapter-related protein complex 4 sigma 1 subunit (Sigma subunit of AP-4) (AP-4 adapter complex sigma subunit) (Swiss-Prot:Q9Y587) (Homo sapiens)
GPI-Anchor Signal predicted for Tb10.61.2500 by DGPI v2.04, no cleavage site predicted
similar to MAP kinase kinase kinase wis4 (EC 2.7.1.-) (MAP kinase kinase kinasewak1) (MAP kinase kinase kinase wik1). (Swiss-Prot:O14299) (Schizosaccharomyces pombe;) curated by M. Parsons, P. Ward, J. Mottram
similar to SP:Q96PY6: Serine/threonine-protein kinase Nek1 (EC 2.7.1.37) (NimA-relatedprotein kinase 1) (NY-REN-55 antigen). {Homo sapiens;} curated by M. Parsons, P. Ward, J. Mottram
proteasome regulatory non-ATPase subunit 8
identical to GB:AAL72631.1: proteasome regulatory non-ATP-ase subunit 8 {Trypanosoma brucei}; similar to 26S proteasome non-ATPase regulatory subunit 7 (26S proteasomeregulatory subunit S12) (Proteasome subunit p40) (Mov34 protein). (Swiss-Prot:
similar to 40S ribosomal protein S2 (Swiss- Prot:P49688) (Arabidopsis thaliana); similar to SP:O43992: 40S ribosomal protein S2. {Leishmania amazonensis}
similar to Syntaxin 72 (AtSYP72) (Swiss- Prot:Q94KK6) (Arabidopsis thaliana)
curated by M. Parsons, P. Ward, J. Mottram curated by M. Parsons, P. Ward, J. Mottram; probably only partial sequence at end of contig
sarcoplasmic reticulum glycoprotein, putative
GPI-Anchor Signal predicted for Tb10.61.1700 by DGPI v2.04 with cleavage site probability 1.5480001 near 1217
intraflagellar transport protein component, putative
synaptobrevin-type vesicle transport protein, putative
similar to Protein transport protein SEC22 (Protein SLY2) (Swiss-Prot:P22214) (Saccharomyces cerevisiae)
RNA polymerase III C11 subunit, putative
contains TFIIS domain; similar to DNA-directed RNA polymerases III 12.5 kDa polypeptide (EC 2.7.7.6) (Swiss-Prot:Q04307) (Saccharomyces cerevisiae)
similar to Myosin light chain kinase (EC 2.7.1.117) (MLCK). (Swiss-Prot:P25323) (Dictyostelium discoideum;) curated by M. Parsons, P. Ward, J. Mottram
hypothetical protein, conserved (pseudogene)
DNA-directed RNA polymerase subunit alpha
U3 snoRNA-associated protein UTP11, putative
signal recognition particle protein, putative
FKBP-type peptidyl-prolyl cis-trans isomerase conserved
similar over 81aa to Early endosome antigen 1 (Endosome-associated protein p162). (Swiss-Prot:Q15075) (Homo sapiens;); UniProt:Q18930 kinase anchor protein {Caenorhabditis elegans;}
tRNA pseudouridine synthase A-like protein
anaphase promoting complex subunit 10-like protein
proteasome regulatory non-ATP-ase subunit 9
likely role in F-actin nucleation as part of complex with ARP3 (curated by B. Wickstead, Univ. of Oxford)
transcription initiation factor TFIID-like protein
similar to Lipase precursor (EC 3.1.1.3) (Triacylglycerol lipase). (Swiss-Prot:P21811) (Rhizopus niveus;Rhizopus oryzae;)
mitogen-activated protein kinase, putative
eukaryotic translation initiation factor 4E
similar to Eukaryotic translation initiation factor 4E type 3 (eIF4E type 3)(eIF-4E type 3) (mRNA cap- binding protein type 3) (Eukaryotictranslation initiation factor 4E-like 3) (eIF4E-like protein 4E-LP). (Swiss- Prot:Q8BMB3) (Mus musculus;)
peptidylprolyl isomerase-like protein, putative
ATP-dependent zinc metallopeptidase, putative
potassium voltage-gated channel, putative
expression site-associated gene 4 (ESAG4) protein, putative
similar to SP:Q26721: Receptor-type adenylate cyclase ESAG 4 (EC 4.6.1.1) (ATPpyrophosphate- lyase) (Adenylyl cyclase) (Expression site- associatedprotein 4) (Antigen 4). {Trypanosoma brucei brucei}
UDP-Gal or UDP-GlcNAc-dependent glycosyltransferase (pseudogene), putative
variant surface glycoprotein (VSG), putative