Anopheles coluzzii mitochondrion, complete genome.
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae; Anophelinae; Anopheles.
Submitted (08-JUL-2022) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (01-JUN-2022) WELLCOME SANGER INSTITUTE, Tree of Life, Wellcome Genome Campus, Hinxton CB10 1SA, United Kingdom
PROVISIONAL REFSEQ: This record has not yet been subject to final\nNCBI review. The reference sequence is identical to OX030894.\nThe assembly AcolN3 is based on 59x PacBio HiFi data, 10X Genomics\nChromium data, and Arima Hi-C data generated by the Anopheles\nReference Genomes project. The assembly process included the\nfollowing sequence of steps: initial PacBio assembly generation\nwith hifiasm, retained haplotig separation with purge_dups,\nshort-read polishing using FreeBayes-called variants from 10X\nGenomics Chromium reads aligned with LongRanger, and Hi-C based\nscaffolding with SALSA2. The mitochondrial genome was assembled\nusing MitoHifi. Finally, the assembly was analysed and manually\nimproved using gEVAL. Chromosome-scale scaffolds confirmed by the\nHi-C data have been named, ordered and oriented based on published\ndata.\nCOMPLETENESS: full length.
TAA stop codon is completed by the addition of 3' A residues to the mRNA