Candidatus Endomicrobium trichonymphae plasmid pTGRD1, complete sequence.
Bacteria; Elusimicrobiota; Endomicrobiia; Endomicrobiales; Endomicrobiaceae; Endomicrobium.
Complete genome of the uncultured Termite Group 1 bacteria in a single host protist cell
Revised annotation of the genome of 'Ca. Endomicrobium trichonymphae' phylotype Rs-D17
Submitted (01-OCT-2007) Contact:Yuichi Hongoh Tokyo Institute of Technology, Department of Life Science and Technology; 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8550, Japan
REFSEQ INFORMATION: The reference sequence is identical to\nAP009511.1.\n\nOn Feb 28, 2013 this sequence version replaced NS_000192.1.\nThe wood-feeding termite Reticulitermes speratus (Isoptera;\nRhinotermitidae) was collected in Saitama, Japan. The entire gut\nwas removed from a worker termite, and a single cell of the\ncellulolytic protist Trichonympha agilis (Parabasalia;\nTrichonymphida) in the gut was physically isolated by using a\nmicromanipulator. In order to collect the intracellular symbionts\nof T. agilis, as-yet-uncultivated bacteria designated phylotype\nRs-D17 belonging to the candidate division Termite Group 1, the\nhost T. agilis cell was ruptured in buffer and bacterial cells\nwhich leaked out were collected with a glass capillary. These\ncollected cells were directly subjected to isothermal whole genome\namplification. After checking their purity by clone analysis of 16S\nrRNA gene and its adjacent ITS, the complete genome sequence was\ndetermined using ABI 3730 capillary sequencers and a GS20 system.\nThe annotation was added by the NCBI Prokaryotic Genome Annotation\nPipeline (PGAP). Information about PGAP can be found here:\nhttps://www.ncbi.nlm.nih.gov/genome/annotation_prok/\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: NCBI RefSeq\nAnnotation Date :: 12/30/2022 00:47:37\nAnnotation Pipeline :: NCBI Prokaryotic Genome\n Annotation Pipeline (PGAP)\nAnnotation Method :: Best-placed reference protein\n set; GeneMarkS-2+\nAnnotation Software revision :: 6.4\nFeatures Annotated :: Gene; CDS; rRNA; tRNA; ncRNA\nGenes (total) :: 1,200\nCDSs (total) :: 1,150\nGenes (coding) :: 1,141\nCDSs (with protein) :: 1,141\nGenes (RNA) :: 50\nrRNAs :: 1, 1, 1 (5S, 16S, 23S)\ncomplete rRNAs :: 1, 1, 1 (5S, 16S, 23S)\ntRNAs :: 45\nncRNAs :: 2\nPseudo Genes (total) :: 9\nCDSs (without protein) :: 9\nPseudo Genes (ambiguous residues) :: 0 of 9\nPseudo Genes (frameshifted) :: 2 of 9\nPseudo Genes (incomplete) :: 4 of 9\nPseudo Genes (internal stop) :: 3 of 9\nCRISPR Arrays :: 2\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
a single cell of termite (Reticulitermes speratus) gut protist
COORDINATES: ab initio prediction:GeneMarkS-2+
Derived by automated computational analysis using gene prediction method: GeneMarkS-2+.