Geobacillus subterraneus strain CPW16 plasmid pBsrF30, complete sequence.
Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Geobacillus.
Genome-Wide Identification of 5-Methylcytosine Sites in Bacterial Genomes By High-Throughput Sequencing of MspJI Restriction Fragments
Submitted (07-APR-2020) Genome Biology, NEB, 240 County Rd, Ipswich, MA 01938, USA
REFSEQ INFORMATION: The reference sequence is identical to\nCP051164.1.\nBacteria and source DNA available from NEB collection 695.\nThe annotation was added by the NCBI Prokaryotic Genome Annotation\nPipeline (PGAP). Information about PGAP can be found here:\nhttps://www.ncbi.nlm.nih.gov/genome/annotation_prok/\n\n##Genome-Assembly-Data-START##\nAssembly Date :: 01-DEC-2017\nAssembly Method :: HGAP v. 2.3.0\nAssembly Name :: BsrF_new_BP9_50_HGAP3_1\nGenome Representation :: Full\nExpected Final Version :: Yes\nGenome Coverage :: 145.47x\nSequencing Technology :: PacBio RSII\n##Genome-Assembly-Data-END##\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: NCBI RefSeq\nAnnotation Date :: 04/28/2023 10:59:32\nAnnotation Pipeline :: NCBI Prokaryotic Genome\n Annotation Pipeline (PGAP)\nAnnotation Method :: Best-placed reference protein\n set; GeneMarkS-2+\nAnnotation Software revision :: 6.5\nFeatures Annotated :: Gene; CDS; rRNA; tRNA; ncRNA\nGenes (total) :: 3,650\nCDSs (total) :: 3,516\nGenes (coding) :: 3,375\nCDSs (with protein) :: 3,375\nGenes (RNA) :: 134\nrRNAs :: 9, 10, 10 (5S, 16S, 23S)\ncomplete rRNAs :: 9, 10, 10 (5S, 16S, 23S)\ntRNAs :: 100\nncRNAs :: 5\nPseudo Genes (total) :: 141\nCDSs (without protein) :: 141\nPseudo Genes (ambiguous residues) :: 0 of 141\nPseudo Genes (frameshifted) :: 41 of 141\nPseudo Genes (incomplete) :: 104 of 141\nPseudo Genes (internal stop) :: 18 of 141\nPseudo Genes (multiple problems) :: 21 of 141\nCRISPR Arrays :: 3\n##Genome-Annotation-Data-END##\nCOMPLETENESS: full length.
COORDINATES: similar to AA sequence:RefSeq:WP_012820773.1
Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: similar to AA sequence:RefSeq:WP_015863237.1
COORDINATES: similar to AA sequence:RefSeq:WP_012820775.1
COORDINATES: similar to AA sequence:RefSeq:WP_012820776.1
COORDINATES: similar to AA sequence:RefSeq:WP_012820777.1
GO:0030420 - establishment of competence for transformation [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_015863241.1
GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_012820779.1
GO:0005524 - ATP binding [Evidence IEA]; GO:0008134 - transcription factor binding [Evidence IEA]
sigma 54-interacting transcriptional regulator
COORDINATES: protein motif:HMM:NF024772.2
GO:0055052 - ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing [Evidence IEA]
GO:0140359 - ABC-type transporter activity [Evidence IEA]
ABC transporter substrate-binding protein
COORDINATES: similar to AA sequence:RefSeq:WP_010869911.1
GO:0005524 - ATP binding [Evidence IEA]; GO:0016887 - ATP hydrolysis activity [Evidence IEA]
COORDINATES: protein motif:HMM:NF012738.2
GO:0005524 - ATP binding [Evidence IEA]; GO:0022857 - transmembrane transporter activity [Evidence IEA]
GO:0055085 - transmembrane transport [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_012820746.1
GO:0016740 - transferase activity [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_012820747.1
COORDINATES: similar to AA sequence:RefSeq:WP_018706488.1
COORDINATES: similar to AA sequence:RefSeq:WP_019533120.1
GO:0009316 - 3-isopropylmalate dehydratase complex [Evidence IEA]
GO:0003861 - 3-isopropylmalate dehydratase activity [Evidence IEA]
GO:0009098 - leucine biosynthetic process [Evidence IEA]
3-isopropylmalate dehydratase large subunit
COORDINATES: similar to AA sequence:RefSeq:WP_012820750.1
3-isopropylmalate dehydratase small subunit
COORDINATES: similar to AA sequence:RefSeq:WP_003353599.1
COORDINATES: similar to AA sequence:RefSeq:WP_009361145.1
COORDINATES: similar to AA sequence:RefSeq:WP_013878226.1
GO:0015074 - DNA integration [Evidence IEA]; GO:0006310 - DNA recombination [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_003321774.1
GO:0003886 - DNA (cytosine-5-)-methyltransferase activity [Evidence IEA]; GO:0008168 - methyltransferase activity [Evidence IEA]; GO:1904047 - S-adenosyl-L-methionine binding [Evidence IEA]
GO:0006304 - DNA modification [Evidence IEA]
COORDINATES: protein motif:HMM:NF019449.2
Cfr10I/Bse634I family restriction endonuclease
COORDINATES: protein motif:HMM:NF024252.2
helix-turn-helix transcriptional regulator
COORDINATES: protein motif:HMM:NF013828.2
COORDINATES: similar to AA sequence:RefSeq:WP_011229840.1
GO:0004803 - transposase activity [Evidence IEA]
frameshifted; internal stop; Derived by automated computational analysis using gene prediction method: Protein Homology.
COORDINATES: similar to AA sequence:RefSeq:WP_011229480.1
COORDINATES: similar to AA sequence:RefSeq:WP_008881775.1
COORDINATES: similar to AA sequence:RefSeq:WP_012820758.1
COORDINATES: similar to AA sequence:RefSeq:WP_011229476.1
GO:0003887 - DNA-directed DNA polymerase activity [Evidence IEA]
GO:0006270 - DNA replication initiation [Evidence IEA]
COORDINATES: similar to AA sequence:RefSeq:WP_015863235.1