Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Caliciviridae; Norovirus.
Norwalk virus N-terminal nonstructural protein is associated with disassembly of the Golgi complex in transfected cells
Identification of further proteolytic cleavage sites in the Southampton calicivirus polyprotein by expression of the viral protease in E. coli
Polyprotein processing in Southampton virus: identification of 3C-like protease cleavage sites by in vitro mutagenesis
Completion of the Norwalk virus genome sequence
Sequence and genomic organization of Norwalk virus
Submitted (01-AUG-2000) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (07-NOV-1995) Michele E. Hardy, Molecular Virology, Baylor College of Medicine, Houston, TX 77006, USA
Submitted (09-AUG-1993) M.K. Estes, Molecular Virology, Baylor College of Medicine, Houston, TX 77006, USA
REVIEWED REFSEQ: This record has been curated by NCBI staff. The\nreference sequence was derived from M87661.\nOn Jun 1, 2006 this sequence version replaced NC_001959.1.\nCOMPLETENESS: full length.
The mature peptides were added by the NCBI staff following publications Liu et all (1996, 1999), that (tentatively) determined processing map by site-directed mutagenesis or by direct sequencing of the cleavage products for closely related Southampton virus (a Norwalk-like virus); ORF1; sequence homologies to 2C helicase, 3C protease, and 3D RNA-dependent RNA polymerase of picornavirus
N-terminal leader p48; 2A2-2B-like membrane protein; distant homolog of H-rev107-like parechovirus 2a2, a putative regulator of cell proliferation; the c-terminal membrane-binding portion contributes to the Golgi disassembly and, therefore, functionally similar to the picornavirus 2B protein
2C-L; probable ortholog of the 2C protein of picornaviruses; the calicivirus NTPase was found in membranous replication complexes
3A-L; located in the polyprotein between NTPase and VPg; second most variable region of the calicivirus polyprotein; the FCV ortholog was detected in membranous replication complexes
For Southampton calicivirus, both N-terminal and C-terminal cleavage sites have been confirmed by direct sequencing. In caliciviruses, VPg can also exist in the form of precursor with the upstream protein which is thought to function as a membrane anchor
Chymotrypsin-like cysteine proteinase; For Southampton calicivirus, both N-terminal and C-terminal cleavage sites have been confirmed by direct sequencing. The FCV proteinase (Pro) can exist in the form of a stable Pro-Pol precursor
the FCV polymerase (Pol) can exist in the form of a stable Pro-Pol precursor; RNA-dependent RNA polymerase
ORF2; 58kd capsid protein; major capsid protein
ORF3; minor structural protein; small basic protein