Melitaea cinxia chromosome 29, ilMelCinx1.1, whole genome shotgun sequence.
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Papilionoidea; Nymphalidae; Nymphalinae; Melitaea.
The Glanville fritillary genome retains an ancient karyotype and reveals selective chromosomal fusions in Lepidoptera
REFSEQ INFORMATION: The reference sequence is identical to\nHG992238.1.\nAssembly name: ilMelCinx1.1\nThe genomic sequence for this RefSeq record is from the\nwhole-genome assembly released by the WELLCOME SANGER INSTITUTE on\n2021/02/16.\n\n##Genome-Assembly-Data-START##\nAssembly Provider :: WELLCOME SANGER INSTITUTE\nAssembly Method :: SOAPdenovo v. 1.05; Newbler v. 2.6; MIP\n Scaffolder v. MARCH-2012\nAssembly Name :: MelCinx1.0\nLong Assembly Name :: Melitaea cinxia 1.0\nGenome Coverage :: 70.0x\nSequencing Technology :: Illumina; 454; SOLiD\n##Genome-Assembly-Data-END##\n\n##Genome-Annotation-Data-START##\nAnnotation Provider :: NCBI\nAnnotation Status :: Full annotation\nAnnotation Name :: Melitaea cinxia Annotation Release\n 100\nAnnotation Version :: 100\nAnnotation Pipeline :: NCBI eukaryotic genome annotation\n pipeline\nAnnotation Software Version :: 9.0\nAnnotation Method :: Best-placed RefSeq; Gnomon\nFeatures Annotated :: Gene; mRNA; CDS; ncRNA\n##Genome-Annotation-Data-END##
Derived by automated computational analysis using gene prediction method: cmsearch.
COORDINATES: nucleotide motif:Rfam:12.0:RF00001
Derived by automated computational analysis using gene prediction method: Gnomon.
COORDINATES: polyA evidence [ECO:0006239]
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 100% coverage of the annotated genomic feature by RNAseq alignments, including 4 samples with support for all annotated introns
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 1 Protein
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 1 Protein, and 100% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 1 Protein, and 26% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 15 Proteins, and 69% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: tRNAscan-SE.
transfer RNA arginine (anticodon ACG); Derived by automated computational analysis using gene prediction method: tRNAscan-SE.
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 100% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins, and 37% coverage of the annotated genomic feature by RNAseq alignments
probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
putative gamma-glutamylcyclotransferase CG2811, transcript variant X1
putative gamma-glutamylcyclotransferase CG2811, transcript variant X2
putative gamma-glutamylcyclotransferase CG2811 isoform X1
putative gamma-glutamylcyclotransferase CG2811 isoform X2
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 100% coverage of the annotated genomic feature by RNAseq alignments, including 2 samples with support for all annotated introns
pyruvate dehydrogenase (acetyl-transferring) kinase, mitochondrial
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 100% coverage of the annotated genomic feature by RNAseq alignments, including 7 samples with support for all annotated introns
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 63% coverage of the annotated genomic feature by RNAseq alignments, including 1 sample with support for all annotated introns
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 12 Proteins
mediator of RNA polymerase II transcription subunit 16
The sequence of the model RefSeq protein was modified relative to this genomic sequence to represent the inferred CDS: substituted 1 base at 1 genomic stop codon; Derived by automated computational analysis using gene prediction method: Gnomon.
LOW QUALITY PROTEIN: mediator of RNA polymerase II transcription subunit 16
gastrula zinc finger protein XlCGF26.1-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 8 Proteins, and 56% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins, and 43% coverage of the annotated genomic feature by RNAseq alignments
gastrula zinc finger protein XlCGF57.1-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 5 Proteins, and 56% coverage of the annotated genomic feature by RNAseq alignments
probable methyltransferase-like protein 25
small nucleolar RNA SNORA16B/SNORA16A family
COORDINATES: nucleotide motif:Rfam:12.0:RF00190
transfer RNA methionine (anticodon CAU); Derived by automated computational analysis using gene prediction method: tRNAscan-SE.
transfer RNA isoleucine (anticodon AAU); Derived by automated computational analysis using gene prediction method: tRNAscan-SE.
ATP-dependent zinc metalloprotease YME1L
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins, and 93% coverage of the annotated genomic feature by RNAseq alignments
androgen-dependent TFPI-regulating protein-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 3 Proteins, and 89% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 8 Proteins, and 69% coverage of the annotated genomic feature by RNAseq alignments
low density lipoprotein receptor adapter protein 1-A
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 8 Proteins, and 17% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 9 Proteins, and 88% coverage of the annotated genomic feature by RNAseq alignments
eukaryotic translation initiation factor 4 gamma-like
The sequence of the model RefSeq transcript was modified relative to this genomic sequence to represent the inferred CDS: deleted 2 bases in 1 codon; Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins
The sequence of the model RefSeq protein was modified relative to this genomic sequence to represent the inferred CDS: deleted 2 bases in 1 codon; Derived by automated computational analysis using gene prediction method: Gnomon.
LOW QUALITY PROTEIN: eukaryotic translation initiation factor 4 gamma-like
3-hydroxy-3-methylglutaryl-coenzyme A reductase
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 7 Proteins, and 84% coverage of the annotated genomic feature by RNAseq alignments, including 4 samples with support for all annotated introns
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 17 Proteins, and 100% coverage of the annotated genomic feature by RNAseq alignments, including 44 samples with support for all annotated introns
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 19 Proteins, and 45% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 4 Proteins
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 5 Proteins
The sequence of the model RefSeq transcript was modified relative to this genomic sequence to represent the inferred CDS: deleted 1 base in 1 codon
The sequence of the model RefSeq protein was modified relative to this genomic sequence to represent the inferred CDS: deleted 1 base in 1 codon; Derived by automated computational analysis using gene prediction method: Gnomon.
LOW QUALITY PROTEIN: ornithine decarboxylase antizyme
glucose-6-phosphatase catalytic subunit 1
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 3 Proteins, and 64% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 18 Proteins
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 4 Proteins, and 86% coverage of the annotated genomic feature by RNAseq alignments
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
adenosine 5'-monophosphoramidase HINT2-like
adenosine 5'-monophosphoramidase HINT1-like
circadian clock-controlled protein daywake-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 90% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 3 Proteins
alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 10 Proteins
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 6 Proteins
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 13 Proteins
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 5 Proteins, and 58% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 9 Proteins
chorion class CA protein ERA.1-like, transcript variant X2
chorion class CA protein ERA.1-like, transcript variant X1
chorion class A proteins Ld24-like isoform X2
chorion class CA protein ERA.1-like isoform X1
chorion class CA protein ERA.1-like, transcript variant X3
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins, and 85% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 1 Protein, and 94% coverage of the annotated genomic feature by RNAseq alignments, including 4 samples with support for all annotated introns
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 19 Proteins
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 100% coverage of the annotated genomic feature by RNAseq alignments, including 116 samples with support for all annotated introns
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 7 Proteins
endoplasmic reticulum junction formation protein lunapark-A-like
transfer RNA tryptophan (anticodon CCA); Derived by automated computational analysis using gene prediction method: tRNAscan-SE.
endoplasmic reticulum junction formation protein lunapark-B-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 3 Proteins, and 44% coverage of the annotated genomic feature by RNAseq alignments
transfer RNA aspartic acid (anticodon GUC); Derived by automated computational analysis using gene prediction method: tRNAscan-SE.
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 4 Proteins, and 18% coverage of the annotated genomic feature by RNAseq alignments
COORDINATES: nucleotide motif:Rfam:12.0:RF00026
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 3 Proteins, and 37% coverage of the annotated genomic feature by RNAseq alignments
bromodomain adjacent to zinc finger domain protein 2B-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 27 Proteins
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 659 Proteins
all trans-polyprenyl-diphosphate synthase PDSS2-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 18 Proteins, and 10% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 1 Protein, and 20% coverage of the annotated genomic feature by RNAseq alignments
spliceosome-associated protein CWC27 homolog
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 100% coverage of the annotated genomic feature by RNAseq alignments, including 3 samples with support for all annotated introns
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 9 Proteins, and 25% coverage of the annotated genomic feature by RNAseq alignments
probable serine/threonine-protein kinase DDB_G0278901
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 4 Proteins, and 20% coverage of the annotated genomic feature by RNAseq alignments
acyl-CoA-binding domain-containing protein 5A
nascent polypeptide-associated complex subunit alpha
LHFPL tetraspan subfamily member 2 protein
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 5 Proteins, and 43% coverage of the annotated genomic feature by RNAseq alignments
isochorismatase domain-containing protein 1-like, transcript variant X1
isochorismatase domain-containing protein 1-like, transcript variant X2
isochorismatase domain-containing protein 1-like isoform X2
isochorismatase domain-containing protein 1-like isoform X1
isochorismatase domain-containing protein 1-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 5 Proteins, and 69% coverage of the annotated genomic feature by RNAseq alignments
coatomer subunit zeta-1, transcript variant X1
coatomer subunit zeta-1, transcript variant X2
coatomer subunit zeta-1, transcript variant X6
coatomer subunit zeta-1, transcript variant X5
coatomer subunit zeta-1, transcript variant X3
coatomer subunit zeta-1, transcript variant X4
tigger transposable element-derived protein 6-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins, and 35% coverage of the annotated genomic feature by RNAseq alignments
transfer RNA glutamic acid (anticodon UUC); Derived by automated computational analysis using gene prediction method: tRNAscan-SE.
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 6 Proteins, and 20% coverage of the annotated genomic feature by RNAseq alignments
transfer RNA asparagine (anticodon GUU); Derived by automated computational analysis using gene prediction method: tRNAscan-SE.
translation machinery-associated protein 7 homolog
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 5 Proteins, and 100% coverage of the annotated genomic feature by RNAseq alignments, including 217 samples with support for all annotated introns
phosphatidylinositol 4-phosphate 5-kinase type-1 gamma-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 78% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins, and 41% coverage of the annotated genomic feature by RNAseq alignments
PTB domain-containing adapter protein ced-6
uncharacterized LOC123667925, transcript variant X2
uncharacterized protein LOC123667925 isoform X2
uncharacterized LOC123667925, transcript variant X1
uncharacterized protein LOC123667925 isoform X1
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins, and 23% coverage of the annotated genomic feature by RNAseq alignments
putative ATP-dependent RNA helicase TDRD12
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 6 Proteins, and 58% coverage of the annotated genomic feature by RNAseq alignments
uncharacterized LOC123668001, transcript variant X2
uncharacterized LOC123668001, transcript variant X1
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 3 Proteins, and 21% coverage of the annotated genomic feature by RNAseq alignments
coiled-coil domain-containing protein 13
transfer RNA valine (anticodon UAC); Derived by automated computational analysis using gene prediction method: tRNAscan-SE.
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 1 Protein, and 34% coverage of the annotated genomic feature by RNAseq alignments
mitochondrial import inner membrane translocase subunit TIM50-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins, and 50% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins, and 84% coverage of the annotated genomic feature by RNAseq alignments, including 49 samples with support for all annotated introns
piggyBac transposable element-derived protein 4-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 45 Proteins, and 23% coverage of the annotated genomic feature by RNAseq alignments
segmentation polarity homeobox protein engrailed-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 46 Proteins, and 11% coverage of the annotated genomic feature by RNAseq alignments
altered inheritance of mitochondria protein 3-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins, and 76% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins, and 100% coverage of the annotated genomic feature by RNAseq alignments
steroidogenic acute regulatory protein-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 6 Proteins, and 76% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 18 Proteins, and 64% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 277 Proteins, and 78% coverage of the annotated genomic feature by RNAseq alignments
guanine nucleotide-binding protein G(f) subunit alpha
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 6 Proteins, and 63% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 2 Proteins, and 8% coverage of the annotated genomic feature by RNAseq alignments
mothers against decapentaplegic homolog 3
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 1 Protein, and 11% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 9 Proteins, and 15% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 11 Proteins, and 45% coverage of the annotated genomic feature by RNAseq alignments
probable inactive tRNA-specific adenosine deaminase-like protein 3
alpha-globin transcription factor CP2-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 57% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 1 Protein, and 62% coverage of the annotated genomic feature by RNAseq alignments
COORDINATES: nucleotide motif:Rfam:12.0:RF00619
differentially expressed in FDCP 8 homolog
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 17 Proteins, and 61% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 30 Proteins
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 1 Protein, and 81% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 18 Proteins, and 7% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 100% coverage of the annotated genomic feature by RNAseq alignments, including 5 samples with support for all annotated introns
transfer RNA alanine (anticodon AGC); Derived by automated computational analysis using gene prediction method: tRNAscan-SE.
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 100% coverage of the annotated genomic feature by RNAseq alignments, including 1 sample with support for all annotated introns
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 63% coverage of the annotated genomic feature by RNAseq alignments, including 4 samples with support for all annotated introns
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 6 Proteins, and 34% coverage of the annotated genomic feature by RNAseq alignments
transfer RNA proline (anticodon CGG); Derived by automated computational analysis using gene prediction method: tRNAscan-SE.
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 11 Proteins
vacuolar protein sorting-associated protein 53 homolog
polypeptide N-acetylgalactosaminyltransferase 1-like
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 3 Proteins, and 100% coverage of the annotated genomic feature by RNAseq alignments, including 2 samples with support for all annotated introns
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 4 Proteins, and 28% coverage of the annotated genomic feature by RNAseq alignments
uncharacterized LOC123667812, transcript variant X1
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 1 Protein, and 72% coverage of the annotated genomic feature by RNAseq alignments
uncharacterized LOC123667812, transcript variant X2
putative leucine-rich repeat-containing protein DDB_G0290503
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 17 Proteins, and 66% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 7 Proteins, and 77% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 11 Proteins, and 80% coverage of the annotated genomic feature by RNAseq alignments
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 80% coverage of the annotated genomic feature by RNAseq alignments, including 54 samples with support for all annotated introns
Derived by automated computational analysis using gene prediction method: Gnomon. Supporting evidence includes similarity to: 10 Proteins, and 73% coverage of the annotated genomic feature by RNAseq alignments