Murray Valley encephalitis virus, complete genome.
Viruses; Riboviria; Flaviviridae; Flavivirus.
NS1' of flaviviruses in the Japanese encephalitis virus serogroup is a product of ribosomal frameshifting and plays a role in viral neuroinvasiveness
Characterization of infectious Murray Valley encephalitis virus derived from a stably cloned genome-length cDNA
Sequence of the 3' half of the Murray Valley encephalitis virus genome and mapping of the nonstructural proteins NS1, NS3, and NS5
Submitted (01-AUG-2000) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (22-JUN-1999) Microbiology, University of Western Australia, Verdun St., Nedlands, Perth, WA 6009, Australia
REVIEWED REFSEQ: This record has been curated by NCBI staff. The\nreference sequence was derived from AF161266.\nThe mature peptides were added at NCBI following annotations\navailable for closely related sequences. Not all of them were\nverified against experimental data available for this virus in the\nliterature - therefore, these mature peptides should still be\nconsidered 'putative'.\nCOMPLETENESS: full length.
post-translationally modified and cleaved into three major structural proteins (C, prM, and E) and seven major non-structural proteins (NS1, NS2a, NS2b, NS3, NS4a, NS4b and NS5)
E/NS1 cleavage site evidence: N-terminal amino acid sequencing, PMID: 1702914
NS2B/NS3 cleavage site evidence: N-terminal amino acid sequencing, PMID: 1702914; component of the virus-encoded protease; nonstructural protein 2B
RNA-helicase; ATPase; component of capping enzyme (RNA thriphosphatase); nonstructural protein 3; protease
product of NS2B-3 protease and signalase
NS4B/NS5 cleavage site evidence: N-terminal amino acid sequencing, PMID: 1702914; nonstructural protein 4B
RNA-dependent RNA polymerase; component of capping enzyme (methyltransferase); nonstructural protein 5
Truncated version of polyprotein that will be produced when frameshifting occurs at nt 3557; Truncated version of polyprotein and NS1' protein have been added and annotated with the kind help of Dr. David Karlin (Department of Zoology, University of Oxford, UK) and Dr. Andrew Firth (Department of Pathology, University of Cambridge, UK)
transframe fusion protein expressed via programmed ribosomal frameshifting; when frameshifting occurs, cleavage between NS1 and NS2A fails to occur