Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Rowavirales; Adenoviridae; Mastadenovirus.
The nucleotide sequence and a first generation gene transfer vector of species B human adenovirus serotype 3
Submitted (04-SEP-2008) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA
Submitted (06-JUN-2005) Institute of Molecular Biology, University of Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland
REVIEWED REFSEQ: This record has been curated by Dr Andrew Davison\n(MRC Virology Unit, Glasgow, UK) and Dr Balazs Harrach (Veterinary\nMedical Research Institute, Budapest, Hungary). The reference\nsequence was derived from DQ086466.\nCURATION:\n Proteins: A standard nomenclature has been applied so that\northologs have the same name. Functional information may have been\npropagated from other adenovirus species.\n Protein-coding regions: The initiation codon for each CDS is\nassigned with as much confidence as possible. CDSs presumably\ninherited from the last common ancestor of all adenoviruses are\nindicated as genus-common, and all other CDSs as genus-specific.\nCDSs that have paralogues in one or more genomes are listed as\nmembers of named families. The evolutionary history of E3 and E4 is\ncomplex, frequently involving generation or loss of paralogues in\nwhole or in part, accompanied by rapid sequence divergence. In E3,\nthis is evident in a set of tandemly arranged CDSs encoding\ntransmembrane proteins. All of these are listed as members of the\nMP family, regardless of whether sequence conservation is\ndetectable.\n Transcripts: In mastadenoviruses, transcription arises from\nthe early genes (E1A, E1B, E2A, E2B, E3 and E4), the intermediate\ngenes (IX and IVa2) and the late genes (L, U and E2A-L). Certain\ntranscriptional features are supported by experimental data for\nsome of these genes in a few adenoviruses (particularly human\nadenovirus C). All transcriptional features not marked as\nexperimentally supported are predictions made on the basis of\nconservation and relevance to CDS expression. The degree of\nconfidence in each prediction depends on the feature under\nconsideration. In primate adenoviruses, predictions for E3, E4 and\nthe 5'-regions of E2A and E2B are generally the least certain. In\nnon-primate mastadenoviruses, this trend is accentuated to the\ndegree that predictions for introns in E3 and E4 are not provided,\nand those for the 5'-regions of E2A, E2B and IVa2 and for the\nintron separating the protein-coding exons of 33K are particularly\ntentative. In human adenovirus C, rightward-oriented transcripts\nfrom the late genes (L1-L5) are polyadenylated close downstream\nfrom polyadenylation signals at five locations (the 3'-ends of the\npIIIa, pX, protease, control protein E3 12.5K and fiber CDSs), and\nthose from the E3 genes (E3A and E3B) at two locations (the 3'-ends\nof the membrane protein E3 CR1-beta and control protein E3 14.7K\nCDSs). Only the polyadenylation signals for L3, E3B and L5 are\nconserved among all mastadenoviruses, whereas those for L1 and L2\nare conserved in most mastadenoviruses and those for L4 and E3A in\na few. In addition, potential additional polyadenylation signals\nexist at the 3'-ends of the penton base, pVII, V, pVI and fiber-2\nCDSs in some mastadenoviruses, particularly those with non-primate\nhosts; the corresponding genes are listed as L1A, L1B, L1C, L2A and\nL5A, respectively. The gene corresponding to E3B is termed E3 in\ngenomes lacking the E3A polyadenylation signal. In some instances\nwhere a TATA signal is not convincingly evident (particularly for\nE2A/E2B), a tentative assignment is made and marked as nominal.\nCOMPLETENESS: full length.
alternative splicing utilizing nonconserved sites may produce additional proteins; genus-specific
between L2 and i leaders; precedes protein 13.6K CDS
i-leader protein; similar CDSs are present in some other human mastadenoviruses, but their conservation may merely be a secondary effect of conservation of DNA polymerase encoded on the opposing strand
covalently attached to genome 5'-termini; genus-common
precedes capsid protein precursor pVIII CDS
precedes protein 33K and encapsidation protein 22K CDSs
precedes hexon assembly protein 100K CDS
precedes capsid protein precursor pVI CDS
precedes core protein precursor pVII CDS
precedes capsid protein precursor pIIIa CDS
precedes DNA polymerase and terminal protein precursor pTP CDSs
putative type 2 membrane protein; product Mu; genus-common
cleaved peptide stimulates protease; genus-common
E2A, E2A-L; precedes single-stranded DNA-binding protein CDS
precedes membrane protein E3 RID-beta CDS
precedes membrane protein E3 RID-alpha CDS
precedes membrane glycoprotein E3 CR1-delta CDS
precedes membrane glycoprotein E3 CR1-gamma CDS
precedes membrane glycoprotein E3 CR1-beta CDS
precedes membrane glycoprotein E3 gp19K CDS
precedes membrane glycoprotein E3 CR1-alpha CDS
0 CR1 domains; genus-specific; MP family
binds to MHC-I and inhibits transport; genus-specific; MP family
exon encoding C terminus unidentified; genus-common
capsid protein IV; genus-common; fiber family