LOCUS       P39765                   181 aa            linear   BCT 03-MAR-2009
DEFINITION  RecName: Full=Bifunctional protein pyrR; Includes: RecName:
            Full=Pyrimidine operon regulatory protein; Includes: RecName:
            Full=Uracil phosphoribosyltransferase; Short=UPRTase.
ACCESSION   P39765
VERSION     P39765.2  GI:20141743
DBSOURCE    UniProtKB: locus PYRR_BACSU, accession P39765;
            class: standard.
            extra accessions:P25982,Q45483
            created: Feb 1, 1995.
            sequence updated: Jan 31, 2002.
            annotation updated: Mar 3, 2009.
            xrefs: M59757.2, AAA21265.2, Z99112.2, CAB13421.1, U48870.1,
            AAB57770.1, B57986, NP_389430.1, 1A3C_A, 1A4X_A, 1A4X_B
            xrefs (non-sequence databases): PDBsum:1A3C, PDBsum:1A4X,
            GeneID:938030, GenomeReviews:AL009126_GR, KEGG:bsu:BSU15470,
            NMPDR:fig|224308.1.peg.1549, SubtiList:BG10712, HOGENOM:P39765,
            BioCyc:BSUB224308:BSU1549-MON, BRENDA:2.4.2.9, GO:0003723,
            GO:0004845, GO:0009116, GO:0006355, GO:0006353, HAMAP:MF_01219,
            InterPro:IPR000836, Pfam:PF00156, PROSITE:PS00103
KEYWORDS    3D-structure; Complete proteome; Glycosyltransferase; RNA-binding;
            Transcription; Transcription regulation; Transcription termination;
            Transferase.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (residues 1 to 181)
  AUTHORS   Quinn,C.L., Stephenson,B.T. and Switzer,R.L.
  TITLE     Functional organization and nucleotide sequence of the Bacillus
            subtilis pyrimidine biosynthetic operon
  JOURNAL   J. Biol. Chem. 266 (14), 9113-9127 (1991)
   PUBMED   1709162
  REMARK    NUCLEOTIDE SEQUENCE [GENOMIC DNA].
            STRAIN=1A610, and JH861
REFERENCE   2  (residues 1 to 181)
  AUTHORS   Turner,R.J., Lu,Y. and Switzer,R.L.
  TITLE     Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr)
            gene cluster by an autogenous transcriptional attenuation mechanism
  JOURNAL   J. Bacteriol. 176 (12), 3708-3722 (1994)
   PUBMED   8206849
  REMARK    SEQUENCE REVISION, AND CHARACTERIZATION.
REFERENCE   3  (residues 1 to 181)
  AUTHORS   Switzer,R.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (??-MAY-1999)
  REMARK    SEQUENCE REVISION TO 34 AND 53.
REFERENCE   4  (residues 1 to 181)
  AUTHORS   Kunst,F., Ogasawara,N., Moszer,I., Albertini,A.M., Alloni,G.,
            Azevedo,V., Bertero,M.G., Bessieres,P., Bolotin,A., Borchert,S.,
            Borriss,R., Boursier,L., Brans,A., Braun,M., Brignell,S.C.,
            Bron,S., Brouillet,S., Bruschi,C.V., Caldwell,B., Capuano,V.,
            Carter,N.M., Choi,S.-K., Codani,J.-J., Connerton,I.F.,
            Cummings,N.J., Daniel,R.A., Denizot,F., Devine,K.M.,
            Duesterhoeft,A., Ehrlich,S.D., Emmerson,P.T., Entian,K.-D.,
            Errington,J., Fabret,C., Ferrari,E., Foulger,D., Fritz,C.,
            Fujita,M., Fujita,Y., Fuma,S., Galizzi,A., Galleron,N., Ghim,S.-Y.,
            Glaser,P., Goffeau,A., Golightly,E.J., Grandi,G., Guiseppi,G.,
            Guy,B.J., Haga,K., Haiech,J., Harwood,C.R., Henaut,A., Hilbert,H.,
            Holsappel,S., Hosono,S., Hullo,M.-F., Itaya,M., Jones,L.-M.,
            Joris,B., Karamata,D., Kasahara,Y., Klaerr-Blanchard,M., Klein,C.,
            Kobayashi,Y., Koetter,P., Koningstein,G., Krogh,S., Kumano,M.,
            Kurita,K., Lapidus,A., Lardinois,S., Lauber,J., Lazarevic,V.,
            Lee,S.-M., Levine,A., Liu,H., Masuda,S., Mauel,C., Medigue,C.,
            Medina,N., Mellado,R.P., Mizuno,M., Moestl,D., Nakai,S., Noback,M.,
            Noone,D., O'Reilly,M., Ogawa,K., Ogiwara,A., Oudega,B., Park,S.-H.,
            Parro,V., Pohl,T.M., Portetelle,D., Porwollik,S., Prescott,A.M.,
            Presecan,E., Pujic,P., Purnelle,B., Rapoport,G., Rey,M.,
            Reynolds,S., Rieger,M., Rivolta,C., Rocha,E., Roche,B., Rose,M.,
            Sadaie,Y., Sato,T., Scanlan,E., Schleich,S., Schroeter,R.,
            Scoffone,F., Sekiguchi,J., Sekowska,A., Seror,S.J., Serror,P.,
            Shin,B.-S., Soldo,B., Sorokin,A., Tacconi,E., Takagi,T.,
            Takahashi,H., Takemaru,K., Takeuchi,M., Tamakoshi,A., Tanaka,T.,
            Terpstra,P., Tognoni,A., Tosato,V., Uchiyama,S., Vandenbol,M.,
            Vannier,F., Vassarotti,A., Viari,A., Wambutt,R., Wedler,E.,
            Wedler,H., Weitzenegger,T., Winters,P., Wipat,A., Yamamoto,H.,
            Yamane,K., Yasumoto,K., Yata,K., Yoshida,K., Yoshikawa,H.-F.,
            Zumstein,E., Yoshikawa,H. and Danchin,A.
  TITLE     The complete genome sequence of the gram-positive bacterium
            Bacillus subtilis
  JOURNAL   Nature 390 (6657), 249-256 (1997)
   PUBMED   9384377
  REMARK    NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
            STRAIN=168
REFERENCE   5  (residues 1 to 181)
  AUTHORS   Pragai,Z., Tjalsma,H., Bolhuis,A., van Dijl,J.M., Venema,G. and
            Bron,S.
  TITLE     The signal peptidase II (Isp) gene of Bacillus subtilis
  JOURNAL   Microbiology (Reading, Engl.) 143 (PT 4), 1327-1333 (1997)
   PUBMED   9141696
  REMARK    NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-173.
            STRAIN=168
REFERENCE   6  (residues 1 to 181)
  AUTHORS   Martinussen,J., Glaser,P., Andersen,P.S. and Saxild,H.H.
  TITLE     Two genes encoding uracil phosphoribosyltransferase are present in
            Bacillus subtilis
  JOURNAL   J. Bacteriol. 177 (1), 271-274 (1995)
   PUBMED   7798145
  REMARK    ENZYME ACTIVITY.
REFERENCE   7  (residues 1 to 181)
  AUTHORS   Turner,R.J., Bonner,E.R., Grabner,G.K. and Switzer,R.L.
  TITLE     Purification and characterization of Bacillus subtilis PyrR, a
            bifunctional pyr mRNA-binding attenuation protein/uracil
            phosphoribosyltransferase
  JOURNAL   J. Biol. Chem. 273 (10), 5932-5938 (1998)
   PUBMED   9488732
  REMARK    CHARACTERIZATION, AND MASS SPECTROMETRY.
REFERENCE   8  (residues 1 to 181)
  AUTHORS   Savacool,H.K. and Switzer,R.L.
  TITLE     Characterization of the interaction of Bacillus subtilis PyrR with
            pyr mRNA by site-directed mutagenesis of the protein
  JOURNAL   J. Bacteriol. 184 (9), 2521-2528 (2002)
   PUBMED   11948166
  REMARK    MUTAGENESIS OF ARG-15; THR-18; ARG-19; HIS-22; ARG-27; THR-41;
            HIS-140; ARG-141; ARG-146 AND LYS-152.
REFERENCE   9  (residues 1 to 181)
  AUTHORS   Tomchick,D.R., Turner,R.J., Switzer,R.L. and Smith,J.L.
  TITLE     Adaptation of an enzyme to regulatory function: structure of
            Bacillus subtilis PyrR, a pyr RNA-binding attenuation protein and
            uracil phosphoribosyltransferase
  JOURNAL   Structure 6 (3), 337-350 (1998)
   PUBMED   9551555
  REMARK    X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
COMMENT     On or before Sep 27, 2005 this sequence version replaced
            gi:11376215, gi:730440.
            [FUNCTION] Regulates transcriptional attenuation of the pyrimidine
            nucleotide (pyr) operon by binding in a uridine-dependent manner to
            specific sites on pyr mRNA. This disrupts an antiterminator hairpin
            in the RNA and favors formation of a downstream transcription
            terminator, leading to a reduced expression of downstream genes.
            [FUNCTION] Also displays a weak uracil phosphoribosyltransferase
            activity which is not physiologically significant.
            [CATALYTIC ACTIVITY] UMP + diphosphate = uracil +
            5-phospho-alpha-D-ribose 1-diphosphate.
            [BIOPHYSICOCHEMICAL PROPERTIES] pH dependence: Optimum pH is 8.2
            for UPRTase activity.
            [SUBUNIT] Homodimer and homohexamer; in equilibrium.
            [MASS SPECTROMETRY] Mass=20263; Mass_error=2; Method=Electrospray;
            Range=1-181; Source=PubMed:9488732.
            [MISCELLANEOUS] Mutagenesis studies identified four amino acid
            residues that seem to be involved directly in binding of the
            protein to pyr mRNA: Thr-18, His-22, Arg-141 and Arg-146. Arg-27
            and Lys-152 were also likely to be involved in RNA-binding, but
            mutations may have altered their subunit-subunit interactions.
            Arg-19 was implicated in pyr regulation, but a specific role in
            RNA-binding could not be demonstrated.
            [MISCELLANEOUS] UMP and UTP incresase the affinity of pyrR for RNA.
            [SIMILARITY] Belongs to the purine/pyrimidine
            phosphoribosyltransferase family. PyrR subfamily.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Bacillus subtilis"
                     /db_xref="taxon:1423"
     gene            1..181
                     /gene="pyrR"
                     /locus_tag="BSU15470"
     Protein         1..181
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /product="Bifunctional protein pyrR"
                     /EC_number="2.4.2.9"
     Region          1..181
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Mature chain"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="Bifunctional protein pyrR. /FTId=PRO_0000183030."
     Region          3..179
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Pribosyltran"
                     /note="Phosphoribosyl transferase domain; cl00309"
                     /db_xref="CDD:119714"
     Region          4..8
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     Region          10..28
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Helical region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     Site            15
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /site_type="mutagenized"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="R->Q: No effect on ability to regulate the pyr
                     operon; no effect on uprtase activity."
     Site            18
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /site_type="mutagenized"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="T->A: No effect on ability to regulate the pyr
                     operon only in presence of excess pyrimidines; reduced
                     affinity for RNA; no effect on UPRTase activity."
     Site            19
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /site_type="mutagenized"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="R->Q: Loss of ability to regulate the pyr operon;
                     no effect on UPRTase activity."
     Site            22
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /site_type="mutagenized"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="H->A: Loss of ability to regulate the pyr operon
                     and to bind to RNA; no effect on UPRTase activity."
     Site            27
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /site_type="mutagenized"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="R->Q: No effect on ability to regulate the pyr
                     operon only in presence of excess pyrimidines; reduced
                     affinity for RNA; no effect on UPRTase activity."
     Region          35..40
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     Region          41..58
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Helical region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     Region          41..42
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Region of interest in the sequence"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Substrate-binding (By similarity)."
     Site            41
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /site_type="mutagenized"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="T->I: Reduced ability to regulate the pyr operon;
                     reduced affinity for RNA; loss of UPRTase activity."
     Region          63..70
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     Region          86..91
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     Region          99..111
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     Region          101..113
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Short sequence motif of biological interest"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="PRPP-binding (By similarity)."
     Region          105..113
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Region of interest in the sequence"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Substrate binding (By similarity)."
     Region          112..124
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Helical region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     Region          128..137
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     Site            138
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /site_type="binding"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Substrate (By similarity)."
     Site            140
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /site_type="mutagenized"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="H->A: Reduced ability to regulate the pyr operon;
                     decreased UPRTase activity."
     Site            141
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /site_type="mutagenized"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="R->Q: Loss of ability to regulate the pyr operon;
                     highly reduced affinity for RNA; no effect on UPRTase
                     activity."
     Region          142..144
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     Site            146
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /site_type="mutagenized"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="R->Q: Reduced ability to regulate the pyr operon,
                     and loss of ability to bind to RNA; no effect on UPRTase
                     activity."
     Region          148..153
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     Site            152
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /site_type="mutagenized"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /note="K->Q: No effect on ability to regulate the pyr
                     operon only in presence of excess pyrimidines; reduced
                     affinity for RNA; no effect on UPRTase activity."
     Region          161..165
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     Site            162
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /site_type="binding"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /note="Substrate (By similarity)."
     Region          167..170
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Helical region"
                     /experiment="experimental evidence, no additional details
                     recorded"
     Region          174..178
                     /gene="pyrR"
                     /locus_tag="BSU15470"
                     /region_name="Beta-strand region"
                     /experiment="experimental evidence, no additional details
                     recorded"
ORIGIN      
        1 mnqkavilde qairraltri ahemiernkg mnncilvgik trgiylakrl aerieqiegn
       61 pvtvgeidit lyrddlskkt sndeplvkga dipvditdqk vilvddvlyt grtvragmda
      121 lvdvgrpssi qlavlvdrgh relpiradyi gkniptskse kvmvqldevd qndlvaiyen
      181 e
//

