LOCUS       MSGEFTUA                1500 bp    DNA     linear   BCT 18-JUN-1999
DEFINITION  Mycobacterium leprae Tuf gene for elongation factor Tu, complete
            cds.
ACCESSION   D13869
VERSION     D13869.1  GI:434782
KEYWORDS    EF-Tu; elongation factor Tu.
SOURCE      Mycobacterium leprae
  ORGANISM  Mycobacterium leprae
            Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
            Corynebacterineae; Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Dhandayuthapani,S., Banu,M.J. and Kashiwabara,Y.
  TITLE     Cloning and sequence determination of the gene coding for the
            elongation factor Tu of Mycobacterium leprae
  JOURNAL   J. Biochem. 115 (4), 664-669 (1994)
   PUBMED   8089081
COMMENT     Submitted (07-DEC-1992) to DDBJ by:
            Subranamian Dhandayuthapani
            National Institute for Leprosy Research
            4-2-1 Aobacho, Higashimurayama-shi
            Tokyo 189
            Japan
            Phone:  0423-91-8211
            Fax:    0423-94-9092.
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Mycobacterium leprae"
                     /mol_type="genomic DNA"
                     /isolate="Thai 53"
                     /db_xref="taxon:1769"
                     /clone_lib="lambda gt11"
     repeat_region   20..71
                     /rpt_type=direct
                     /rpt_unit="20..29"
                     /rpt_unit="62..71"
     repeat_region   90..231
                     /rpt_type=direct
                     /rpt_unit="90..99"
                     /rpt_unit="222..231"
     RBS             205..210
                     /standard_name="Shine-Dalgarno sequence"
     gene            219..1409
                     /gene="Tuf"
     CDS             219..1409
                     /gene="Tuf"
                     /function="peptide chain elongtion during protein
                     synthesis"
                     /standard_name="EF-Tu"
                     /note="start codon gtg"
                     /codon_start=1
                     /evidence=not_experimental
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="BAA02982.2"
                     /db_xref="GI:5103687"
                     /translation="MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPNLN
                     ESRAFDQIDNAPEERQRGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMD
                     GAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKSDAVDDEELLELVEMEVRELL
                     AAQEFDEDAPVVRVSALKALEGDAKWVESVTQLMDAVDESIPAPVRETDKPFLMPVED
                     VFTITGRGTVVTGRVERGVVNVNEEVEIVGIRQTTTKTTVTGVEMFRKLLDQGQAGDN
                     VGLLLRGIKREDVERGQVVIKPGTTTPHTEFEGQVYILSKDEGGRHTPFFNNYRPQFY
                     FRTTDVTGVVTLPEGTEMVMPGDNTNISVTLIQPVAMDEGLRFAIREGGPTVGAGRVV
                     KIIK"
     repeat_region   465..729
                     /rpt_type=direct
                     /rpt_unit="465..474"
                     /rpt_unit="720..729"
     repeat_region   883..913
                     /rpt_type=direct
                     /rpt_unit="883..892"
                     /rpt_unit="904..913"
     repeat_region   1169..1369
                     /rpt_type=direct
                     /rpt_unit="1169..1180"
                     /rpt_unit="1358..1369"
ORIGIN      
        1 gctcggtcca agacgcaagg ccggcgaact actccatggt gttcaactcg tactccgaag
       61 tgccggcgaa cgtgtcgaag gagatcatcg cgaaggcgac gggcgagtag gagttgagtg
      121 gtcggaaaag cctacgagtt atgctgacgc ggtcactacc gcggataaat tactaacatt
      181 gctttttaca agcaccaata gtccaggagg acacagaagt ggcgaaggcg aagttcgagc
      241 ggacgaagcc gcacgtcaac atcgggacca tcggtcacgt tgaccacggc aagaccacat
      301 tgaccgcggc tattaccaag gtcctgcatg acaagttccc caacctgaat gagtcgcgcg
      361 cctttgacca gattgacaac gcgcccgagg agcgtcagcg cggtatcacc atcaacattt
      421 cccacgtgga gtatcagacc gagaagcgtc actatgctca cgtcgacgcc ccggggcacg
      481 ccgactacat taagaacatg atcaccggtg cggcccagat ggatggtgcg attctggtgg
      541 tcgctgctac agacggcccg atgccgcaga ctcgcgagca cgtgctgctc gctcgtcagg
      601 tgggtgtacc ttacatcctg gtcgcactta acaagtccga cgccgtggac gacgaggaac
      661 tactcgagct tgtcgagatg gaagtccgtg agttgctggc tgcccaggaa ttcgacgagg
      721 acgccccggt tgtgcgtgtc tcggcattga aggcgctcga gggtgacgcc aagtgggtcg
      781 agtctgtcac acagttgatg gacgctgtcg acgagtcgat cccggctcca gtccgcgaga
      841 ctgacaagcc gttcctgatg cccgttgagg acgtcttcac tatcaccggt cgtggcaccg
      901 tggtcaccgg tcgggtggag cgcggcgtgg tcaacgtgaa cgaggaagtt gagatcgtcg
      961 gcattcgtca gacgaccacc aagaccaccg tcacgggtgt ggaaatgttt cgtaagctgc
     1021 tcgaccaagg tcaggccggt gataacgttg gtctgttgtt gcgtggcatc aagcgcgagg
     1081 acgtcgagcg tggtcaggtg gtcatcaaac ccggcaccac caccccgcat accgagttcg
     1141 aaggccaagt atacatcctg tccaaggacg aaggtggtcg gcacacaccg ttcttcaaca
     1201 actaccgtcc gcagttctac ttccgtacca ctgatgtgac cggtgtggtg actttgccgg
     1261 agggcaccga gatggtgatg ccaggtgaca acaccaacat ctcggtgacg ttgattcagc
     1321 ccgttgccat ggacgagggt ttgcgcttcg cgatccgcga aggtggtccg actgttggtg
     1381 ccggccgggt cgtcaagatc atcaagtaag tcgcatcaac gggccaggtg gttacaacgg
     1441 tccacatttg cgaaacggcg ctcatccgaa aggataggcg cgtttctttt gagtttatgc
//

