LOCUS       PNECG                   2355 bp    DNA     circular BCT 21-APR-1996
DEFINITION  Plasmid pNE131 (from S.epidermidis), complete genome.
ACCESSION   M12730
VERSION     M12730.1  GI:150829
KEYWORDS    MLS antibiotics resistance; complete genome;
            macrolide-lincosamide-streptogramin B resistance; plasmid;
            replication and maintenance protein.
SOURCE      Plasmid pNE131
  ORGANISM  Plasmid pNE131
            other sequences; plasmids; Plasmid.
REFERENCE   1  (bases 696 to 1586)
  AUTHORS   Lampson,B.C. and Parisi,J.T.
  TITLE     Naturally occurring Staphylococcus epidermidis plasmid expressing
            constitutive macrolide-lincosamide-streptogramin B resistance
            contains a deleted attenuator
  JOURNAL   J. Bacteriol. 166 (2), 479-483 (1986)
   PUBMED   3084450
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Lampson,B.C. and Parisi,J.T.
  TITLE     Nucleotide sequence of the constitutive
            macrolide-lincosamide-streptogramin B resistance plasmid pNE131
            from Staphylococcus epidermidis and homologies with Staphylococcus
            aureus plasmids pE194 and pSN2
  JOURNAL   J. Bacteriol. 167 (3), 888-892 (1986)
   PUBMED   3091582
COMMENT     Original source text: Plasmid pNE131 (clone: pNE131.) DNA. Draft
            entry and clean copy sequence for [2] kindly provided by
            J.T.Parisi, 04-AUG-1986. Ribosome binding sites are located at
            positions 754-759, 769-773, and 1944-1948; a -35 region at
            1889-1899, and -10 regions at 733-738, and 1927-1932.
FEATURES             Location/Qualifiers
     source          1..2355
                     /db_xref="taxon:2608"
                     /mol_type="genomic DNA"
                     /clone="pNE131."
                     /specific_host="Staphylococcus epidermidis"
                     /plasmid="Plasmid pNE131"
                     /organism="Plasmid pNE131"
     CDS             join(1959..2355,1..92)
                     /db_xref="GI:150830"
                     /codon_start=1
                     /protein_id="AAA98295.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIV
                     QLISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEE
                     GNIIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALYLIIILSRTS
                     IT"
                     /product="replication and maintenance protein"
                     /transl_table=11
     gene            706..1550
                     /gene="ermM"
     -35_signal      706..715
                     /gene="ermM"
     -10_signal      733..738
                     /gene="ermM"
     mRNA            746..1550
                     /gene="ermM"
     CDS             780..1514
                     /protein_id="AAA98296.1"
                     /gene="ermM"
                     /transl_table=11
                     /db_xref="GI:150831"
                     /codon_start=1
                     /product="macrolide-lincosamide-streptogramin
                     B-resistance protein"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF
                     TLELVQRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNI
                     PYNISTDIIRKIVFDSIADEIYLIVEYGFAKRLLNTKRSFALFLMAEVDISILSMVPR
                     EYFHPKPKVNSSLIRLNRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH
                     AGIDDLNNISFEQFLSLFNSYKLFNK"
     terminator      1513..1546
                     /gene="ermM"
                     /note="putative"
BASE COUNT      911 a    359 c    371 g    714 t
ORIGIN      1 bp upstream of TaqI site.
        1 tcgaatttgg gaactttgag caagaggcaa atgaaaaaca agaaaatgca ctttatctga
       61 ttattattct ttcaaggact agtataacat aaatcgtcta caaatagaca aaaaacctgc
      121 acgcttaatg tagatcaaaa gcttaacgca aatgaaatag attgacctcc caataacacc
      181 acgttagtta ttgggagtca atctatgaaa tgcgattaag ctttttctaa ttcacataag
      241 cgtgcaggtt taaagtacat aaaaaatata atgaaaaaaa gcatcattat actaacgtta
      301 taccaacatt atactaattg cttattccaa tttcctattg gttggaacca acaggcgtta
      361 gtgtgttgtt gagttggtac tttcatggga ttaatcccat gaaaccccca accaactcgc
      421 caaagctttg gctaacacac acgccattcc aaccaatagt tttctcggca ttaaagccat
      481 gctctgacgc ttaaatgcac taatgcctta aaaaaacatt aaagtctaac acactagact
      541 tattttcatt cgtaattaag tcgttaaacc gtgtgctcta cgaccaaaag tataaaacct
      601 ttaagaactt tcttttttct tgtaaaaaaa gaaactagat aaatctctca tatcttttat
      661 tcaataatcg catcagattg cagtataaat ttaacgatca ctcatcatgt tcatatttat
      721 cagagctcgt gctataatta tactaatttt ataaggagga aaaaataaag agggttataa
      781 tgaacgagaa aaatataaaa cacagtcaaa actttattac ttcaaaacat aatatagata
      841 aaataatgac aaatataaga ttaaatgaac atgataatat ctttgaaatc ggctcaggaa
      901 aagggcattt tacccttgaa ttagtacaga ggtgtaattt cgtaactgcc attgaaatag
      961 accataaatt atgcaaaact acagaaaata aacttgttga tcacgataat ttccaagttt
     1021 taaacaagga tatattgcag tttaaatttc ctaaaaacca atcctataaa atatttggta
     1081 atatacctta taacataagt acggatataa tacgcaaaat tgtttttgat agtatagctg
     1141 atgagattta tttaatcgtg gaatacgggt ttgctaaaag attattaaat acaaaacgct
     1201 cattcgcatt atttttaatg gcagaagttg atatttctat attaagtatg gttccaagag
     1261 aatattttca tcctaaacct aaagtgaata gctcacttat cagattaaat agaaaaaaat
     1321 caagaatatc acacaaagat aaacagaagt ataattattt cgttatgaaa tgggttaaca
     1381 aagaatacaa gaaaatattt acaaaaaatc aatttaacaa ttccttaaaa catgcaggaa
     1441 ttgacgattt aaacaatatt agctttgaac aattcttatc tcttttcaat agctataaat
     1501 tatttaataa gtaagttaag ggatgcataa actgcatcct taacttgttt ttcgtgtacc
     1561 tattttttgt gaatcgatta tgtcttttgc gcattcactt cttttctata taaatatgag
     1621 cgacagataa gcgtcggaaa agcagcaaaa agtttccttt ttgctgttgg agcatggggg
     1681 ttcagggggt gcagtatctg acgtcaatgc cgagcgaaag cgagccgaag ggtagcattt
     1741 acgttagata accccctgat atgctccgac gctttatata gaaaagaaga ttcaactagg
     1801 taaaatctta atataggttg agatgataag gtttataagg aatttgtttg ttctaatttt
     1861 tcactcattt tgttctaatt tcttttaaca aatgttcttt tttttttaga acagttatga
     1921 tatagttaga atagtttaaa ataaggagtg agaaaaagat gaaagaaaga tatggaacag
     1981 tctataaagg ctctcagagg ctcatagacg aagaaagtgg agaagtcata gaggtagaca
     2041 agttataccg taaacaaacg tctggtaact tcgtaaaggc atatatagtg caattaataa
     2101 gtatgttaga tatgattggc ggaaaaaaac ttaaaatcgt taactatatc ctagataatg
     2161 tccacttaag taacaataca atgatagcta caacaagaga aatagcaaaa gctacaggaa
     2221 caagtctaca aacagtaata acaacactta aaatcttaga agaaggaaat attataaaaa
     2281 gaaaaactgg agtattaatg ttaaaccctg aactactaat gagaggcgac gaccaaaaac
     2341 aaaaatacct cttac
//
