plasma membrane
type: CellularLocationVocabulary
xref: UnificationXref29

Assembly of M2 tetramers
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/10508393
xref: UnificationXref164
xref: UnificationXref165
xref: http://identifiers.org/pubmed/11087364
participant stoichiometry: Stoichiometry64
left: M2
comment: The M2 from influenza A virus is a 97-residue protein with a single transmembrane helix that associates to form a tetramer in the endoplasmic reticulum (Salom et al, 2000). A 15-20-residue segment C-terminal to the membrane-spanning region has been postulated to aid in the stabilization of the tetrameric assembly (Kochendoerfer et al 1999).
comment: Authored: Steel, J, 2007-04-30 20:49:24
comment: Reviewed: Squires, B, Rush, MG, 2007-04-30 20:48:45
right: M2 Tetramer

Binding of M1 to vRNP
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/14722281
xref: http://identifiers.org/pubmed/12490406
xref: http://identifiers.org/pubmed/10438836
xref: http://identifiers.org/pubmed/10644350
xref: http://identifiers.org/pubmed/11531417
xref: UnificationXref153
xref: UnificationXref152
xref: http://identifiers.org/pubmed/11222100
left: M1
left: vRNP destined for Export
comment: Authored: Bortz, E, Garcia-Sastre, A, 2007-02-12 19:39:05
comment: M1 protein binds to viral RNP through its C-terminal domain (Baudin, 2001). The influenza M1 protein accumulates in the infected cell nucleus through a nuclear localization signal (NLS) RKLKR (residues 101-105) in its N-terminus (Ye, 1999). A host cell protein, HSP70, is thought to inhibit M1 binding at nonpermissive temperatures (Hirayama et al., 2004).
comment: Reviewed: Squires, B, 2007-02-12 19:39:22
right: vRNP:M1 for Export

Transport of processed viral proteins to the cell membrane
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/10890900
xref: UnificationXref53
xref: UnificationXref52
left: Glycosylated, palmitylated and folded HA trimer:Lipid Raft Complex
left: palmitylated M2 Tetramer
left: Gycosylated NA Tetramer:Lipid Raft
left: NP:Lipid Raft
comment: Once processed, the viral proteins are transported from the golgi apparatus to the plasma membrane.
comment: Authored: Steel, J, 2007-04-30 20:49:24
comment: Reviewed: Squires, B, Rush, MG, 2007-04-30 20:48:45
right: Glycosylated, palmitylated and folded HA trimer:Lipid Raft Complex
right: NP:Lipid Raft
right: Gycosylated NA Tetramer:Lipid Raft
right: palmitylated M2 Tetramer

Viral Polymerase Assembly
type: BiochemicalReaction
data source: Reactome
xref: UnificationXref111
xref: http://identifiers.org/pubmed/17121807
xref: http://identifiers.org/pubmed/17005651
xref: UnificationXref112
xref: http://identifiers.org/pubmed/15308710
xref: http://identifiers.org/pubmed/11483758
xref: http://identifiers.org/pubmed/8948635
xref: http://identifiers.org/pubmed/3023071
xref: http://identifiers.org/pubmed/17081640
xref: http://identifiers.org/pubmed/3783823
left: PA
left: PB2
left: PB1
comment: The mature ternary influenza viral polymerase complex consists of PB1, PB2, and PA. The N-terminus of PB1 (residues 1-48) interacts with PB2, and amino acids 506-659 in PB1 interact with the PA subunit (Gonzalez, 1996; Perez, 2001). Although monomeric PB1, PB2 and PA, as well as PB1-PB2 and PB1-PA dimers are likely to exist in infected cells, it is believed that most of the polymerase proteins are assembled into the trimeric PB1-PB2-PA complex (Detjen, 1987). Newly synthesized subunits of the polymerase are imported into the nucleus through nuclear localization signals (NLS), which interact with cellular importin family proteins (Jones, 1986; Buolo, 2006). Importin beta-3 (Ran binding protein 5) facilitates nuclear import of PB1 and a PB1-PA dimer (Deng, 2006); coexpression of PA with PB1 was shown to enhance the import of PB1 (Fodor, 2004). A PB1-PB2 dimer has been found to interact with the molecular chaperone heat shock protein 90 (HSP90) to facilitate import (Naito, 2007). The three subunits assembled in the nucleus form a mature ternary polymerase complex that binds viral vRNA or cRNA (Jones, 1986; Buolo, 2006).
comment: Authored: Bortz, E, Garcia-Sastre, A, 2007-02-12 19:39:05
comment: Reviewed: Squires, B, 2007-02-12 19:39:22
right: Viral RNA dependent RNA polymerase

Association with M1 at cell membrane
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/15567494
xref: UnificationXref100
xref: http://identifiers.org/pubmed/15298170
xref: UnificationXref101
xref: http://identifiers.org/pubmed/15731254
xref: PublicationXref2
participant stoichiometry: Stoichiometry21
participant stoichiometry: Stoichiometry20
participant stoichiometry: Stoichiometry22
participant stoichiometry: Stoichiometry34
participant stoichiometry: Stoichiometry32
participant stoichiometry: Stoichiometry23
participant stoichiometry: Stoichiometry33
participant stoichiometry: Stoichiometry19
participant stoichiometry: Stoichiometry35
left: Gycosylated NA Tetramer
left: Glycosylated, palmitylated and folded HA trimer
left: RNP pre-assembly complex
left: PB2
left: PA
left: M1
left: PB1
left: palmitylated M2 Tetramer
left: NEP/NS2
left: NP
comment: Authored: Marsh, G, 2007-04-30 20:49:40
comment: As influenza viruses bud from the plasma membrane of infected cells, complete virions are not seen inside cells. In polarized epithelial cells, assembly and budding of influenza occurs from the apical plasma membrane (Schmitt, 2004). For efficient assembly, all virion components must accumulate at the budding site, and it is believed that the viral glycoprotein accumulation determines the site of virus assembly and budding (Nayak, 2004). M1 is thought to be the bridge between the envelope glycoproteins and the RNPs for assembly (Schmitt, 2004). M2 is also required, because if it is not present RNPs are not packaged into budding virions (McCown, 2005), however it role is not known.
comment: Reviewed: Squires, B, Rush, MG, 2007-04-30 20:48:45
right: Matrix associated RNP pre-assembly complex

Genomic RNA Segment 8
type: Rna
entity reference: H1N1 Genomic RNA Segment 8
data source: Reactome
xref: UnificationXref140
xref: UnificationXref141
cellular location: nucleoplasm
comment: Reactome DB_ID: 188839

Genomic RNA Segment 7
type: Rna
entity reference: H1N1 Genomic RNA Segment 7
data source: Reactome
xref: UnificationXref139
xref: UnificationXref138
cellular location: nucleoplasm
comment: Reactome DB_ID: 188863

Genomic RNA Segment 6
type: Rna
entity reference: H1N1 Genomic RNA Segment 6
data source: Reactome
xref: UnificationXref137
xref: UnificationXref136
cellular location: nucleoplasm
comment: Reactome DB_ID: 188868

Genomic RNA Segment 5
type: Rna
entity reference: H1N1 Genomic RNA Segment 5
data source: Reactome
xref: UnificationXref135
xref: UnificationXref134
cellular location: nucleoplasm
comment: Reactome DB_ID: 188858

Genomic RNA Segment 4
type: Rna
entity reference: H1N1 Genomic RNA Segment 4
data source: Reactome
xref: UnificationXref133
xref: UnificationXref132
cellular location: nucleoplasm
comment: Reactome DB_ID: 188867

Genomic RNA Segment 3
type: Rna
entity reference: EMBL:AF389117 H1N1 Genomic RNA Segment 3
data source: Reactome
xref: UnificationXref131
xref: UnificationXref130
cellular location: nucleoplasm
comment: Reactome DB_ID: 188826

Genomic RNA Segment 2
type: Rna
entity reference: EMBL:AF389116 H1N1 Genomic RNA Segment 2
data source: Reactome
xref: UnificationXref128
xref: UnificationXref129
cellular location: nucleoplasm
comment: Reactome DB_ID: 188827

Genomic RNA Segment 1
type: Rna
entity reference: EMBL:AF389115 H1N1 Genomic RNA Segment 1
data source: Reactome
xref: UnificationXref126
xref: UnificationXref127
cellular location: nucleoplasm
comment: Reactome DB_ID: 188875

http://identifiers.org/pubmed/11222100
type: PublicationXref
id: 11222100
author: Ruigrok, RW
author: Petit, I
author: Weissenhorn, W
author: Baudin, F
db: Pubmed
title: In vitro dissection of the membrane and RNP binding activities of influenza virus M1 protein
source: Virology 281:102-8
year: 2001

http://identifiers.org/pubmed/11087364
type: PublicationXref
id: 11087364
author: DeGrado, WF
author: Salom, D
author: Hill, BR
author: Lear, JD
db: Pubmed
title: pH-dependent tetramerization and amantadine binding of the transmembrane helix of M2 from the influenza A virus
source: Biochemistry 39:14160-70
year: 2000

http://identifiers.org/pubmed/15731254
type: PublicationXref
id: 15731254
author: McCown, MF
author: Pekosz, A
db: Pubmed
title: The influenza A virus M2 cytoplasmic tail is required for infectious virus production and efficient genome packaging
source: J Virol 79:3595-605
year: 2005

Stoichiometry18
type: Stoichiometry
physical entity: palmitylated M2
stoichiometric coefficient: 4.0

Stoichiometry17
type: Stoichiometry
physical entity: Glycosylated, palmitylated and folded HA trimer
stoichiometric coefficient: 1.0

http://identifiers.org/pubmed/11483758
type: PublicationXref
id: 11483758
author: Donis, RO
author: Perez, DR
db: Pubmed
title: Functional analysis of PA binding by influenza a virus PB1: effects on polymerase activity and viral infectivity
source: J Virol 75:8127-36
year: 2001

Stoichiometry19
type: Stoichiometry
physical entity: PB2
stoichiometric coefficient: 37.0

PB2
type: ProteinReference
name: UniProt:P03428 PB2
xref: UnificationXref55
comment: SUBUNIT Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.SIMILARITY Belongs to the influenza viruses PB2 family.
organism: Influenza A virus

Stoichiometry14
type: Stoichiometry
physical entity: Gycosylated NA Tetramer
stoichiometric coefficient: 1.0

Stoichiometry13
type: Stoichiometry
physical entity: Glycosylated NA
stoichiometric coefficient: 4.0

Stoichiometry16
type: Stoichiometry
physical entity: HA folded, glycosylated, and palmitylated
stoichiometric coefficient: 3.0

Stoichiometry15
type: Stoichiometry
physical entity: Lipid Raft
stoichiometric coefficient: 1.0

Stoichiometry10
type: Stoichiometry
physical entity: Lipid Raft
stoichiometric coefficient: 1.0

Stoichiometry12
type: Stoichiometry
physical entity: Lipid Raft
stoichiometric coefficient: 1.0

Stoichiometry11
type: Stoichiometry
physical entity: NP
stoichiometric coefficient: 1.0

Stoichiometry30
type: Stoichiometry
physical entity: NEP/NS2
stoichiometric coefficient: 8.0

Lipid Raft
type: PhysicalEntity
data source: Reactome
xref: UnificationXref30
xref: UnificationXref31
cellular location: plasma membrane
comment: Reactome DB_ID: 195731

http://identifiers.org/pubmed/15567494
type: PublicationXref
id: 15567494
author: Barman, S
author: Nayak, DP
author: Hui, EK
db: Pubmed
title: Assembly and budding of influenza virus
source: Virus Res 106:147-65
year: 2004

Lipid Raft
type: PhysicalEntity
data source: Reactome
xref: UnificationXref9
xref: UnificationXref8
cellular location: Golgi membrane
comment: Reactome DB_ID: 195764

EMBL:AF389116 H1N1 Genomic RNA Segment 2
type: RnaReference
name: H1N1 Genomic RNA Segment 2
xref: UnificationXref83
comment: Influenza A virus (A/Puerto Rico/8/34/Mount Sinai(H1N1)) segment 2, complete sequence.
organism: Influenza A virus

H1N1 Genomic RNA Segment 6
type: RnaReference
name: EMBL:AF389120 H1N1 Genomic RNA Segment 6
xref: UnificationXref80
comment: Influenza A virus (A/Puerto Rico/8/34/Mount Sinai(H1N1)) segment 6, complete sequence.
organism: Influenza A virus

'Importin beta-3 [nucleoplasm]' positively regulates 'Viral Polymerase Assembly'
type: Control
data source: Reactome
xref: UnificationXref119
xref: UnificationXref120
controlled: Viral Polymerase Assembly
control type: ACTIVATION
controller: Ran binding protein-5

H1N1 Genomic RNA Segment 8
type: RnaReference
name: EMBL:AF389122 H1N1 Genomic RNA Segment 8
xref: UnificationXref75
comment: Influenza A virus (A/Puerto Rico/8/34/Mount Sinai(H1N1)) segment 8, complete sequence.
organism: Influenza A virus

'HSP70' negatively regulates 'Binding of M1 to vRNP'
type: Control
data source: Reactome
xref: UnificationXref155
xref: UnificationXref154
controlled: Binding of M1 to vRNP
control type: INHIBITION
controller: heat shock 70kDa protein 1

H1N1 Genomic RNA Segment 4
type: RnaReference
name: EMBL:AF389118 H1N1 Genomic RNA Segment 4
xref: UnificationXref64
comment: Influenza A virus (A/Puerto Rico/8/34/Mount Sinai(H1N1)) segment 4, complete sequence.
organism: Influenza A virus

EMBL:AF389115 H1N1 Genomic RNA Segment 1
type: RnaReference
name: H1N1 Genomic RNA Segment 1
xref: UnificationXref95
comment: Influenza A virus (A/Puerto Rico/8/34/Mount Sinai(H1N1)) segment 1, complete sequence
organism: Influenza A virus

EMBL:AF389117 H1N1 Genomic RNA Segment 3
type: RnaReference
name: H1N1 Genomic RNA Segment 3
xref: UnificationXref92
comment: Influenza A virus (A/Puerto Rico/8/34/Mount Sinai(H1N1)) segment 3, complete sequence.
organism: Influenza A virus

H1N1 Genomic RNA Segment 5
type: RnaReference
name: EMBL:AF389119 H1N1 Genomic RNA Segment 5
xref: UnificationXref89
comment: Influenza A virus (A/Puerto Rico/8/34/Mount Sinai(H1N1)) segment 5, complete sequence.
organism: Influenza A virus

H1N1 Genomic RNA Segment 7
type: RnaReference
name: EMBL:AF389121 H1N1 Genomic RNA Segment 7
xref: UnificationXref86
comment: Influenza A virus (A/Puerto Rico/8/34/Mount Sinai(H1N1)) segment 7, complete sequence.
organism: Influenza A virus

http://identifiers.org/pubmed/17121807
type: PublicationXref
id: 17121807
author: Momose, F
author: Naito, T
author: Nagata, K
author: Kawaguchi, A
db: Pubmed
title: Involvement of hsp90 in assembly and nuclear import of influenza virus RNA polymerase subunits
source: J Virol 81:1339-49
year: 2007

Homo sapiens
type: BioSource
xref: UnificationXref115

'HSP90 [nucleoplasm]' positively regulates 'Viral Polymerase Assembly'
type: Control
data source: Reactome
xref: UnificationXref114
xref: UnificationXref113
controlled: Viral Polymerase Assembly
control type: ACTIVATION
controller: HSP90AA1

Stoichiometry29
type: Stoichiometry
physical entity: PA
stoichiometric coefficient: 8.0

Influenza A virus
type: BioSource
xref: UnificationXref2

Stoichiometry28
type: Stoichiometry
physical entity: M1
stoichiometric coefficient: 8.0

Stoichiometry27
type: Stoichiometry
physical entity: NP
stoichiometric coefficient: 24.0

Stoichiometry26
type: Stoichiometry
physical entity: Genomic RNA Segment 4
stoichiometric coefficient: 1.0

Stoichiometry25
type: Stoichiometry
physical entity: PB1
stoichiometric coefficient: 8.0

Stoichiometry24
type: Stoichiometry
physical entity: PB2
stoichiometric coefficient: 8.0

Stoichiometry23
type: Stoichiometry
physical entity: M1
stoichiometric coefficient: 2992.0

Stoichiometry22
type: Stoichiometry
physical entity: NP
stoichiometric coefficient: 976.0

Stoichiometry21
type: Stoichiometry
physical entity: Gycosylated NA Tetramer
stoichiometric coefficient: 100.0

Stoichiometry20
type: Stoichiometry
physical entity: PB1
stoichiometric coefficient: 37.0

SequenceSite2
type: SequenceSite
position status: EQUAL
sequence position: 732

SequenceSite1
type: SequenceSite
position status: EQUAL
sequence position: 2

NP
type: Protein
entity reference: UniProt:P03466 NP
data source: Reactome
xref: UnificationXref14
xref: UnificationXref13
cellular location: Golgi membrane
comment: Reactome DB_ID: 195740

palmitylated M2
type: Protein
entity reference: UniProt:P06821 M
data source: Reactome
xref: UnificationXref19
xref: UnificationXref18
cellular location: Golgi membrane
comment: Reactome DB_ID: 195741

Glycosylated NA
type: Protein
entity reference: UniProt:P03468 NA
data source: Reactome
xref: UnificationXref4
xref: UnificationXref5
cellular location: Golgi membrane
comment: Reactome DB_ID: 195751

Glycosylated NA
type: Protein
entity reference: UniProt:P03468 NA
data source: Reactome
xref: UnificationXref36
xref: UnificationXref37
cellular location: plasma membrane
comment: Reactome DB_ID: 195750

PublicationXref2
type: PublicationXref
id: 15298170
author: Schmitt, AP
author: Lamb, RA
db: Pubmed
title: Escaping from the cell: assembly and budding of negative-strand RNA viruses
source: Curr Top Microbiol Immunol 283:145-96
year: 2004

HA folded, glycosylated, and palmitylated
type: Protein
entity reference: UniProt:P03452 HA
data source: Reactome
xref: UnificationXref43
xref: UnificationXref42
cellular location: plasma membrane
comment: Reactome DB_ID: 195762

HA folded, glycosylated, and palmitylated
type: Protein
entity reference: UniProt:P03452 HA
data source: Reactome
xref: UnificationXref24
xref: UnificationXref23
cellular location: Golgi membrane
comment: Reactome DB_ID: 195743

NP
type: Protein
entity reference: UniProt:P03466 NP
data source: Reactome
xref: UnificationXref32
xref: UnificationXref33
cellular location: plasma membrane
comment: Reactome DB_ID: 195752

PB2
type: Protein
entity reference: PB2
data source: Reactome
xref: UnificationXref57
xref: UnificationXref56
cellular location: cytosol
comment: Reactome DB_ID: 195946

palmitylated M2
type: Protein
entity reference: UniProt:P06821 M
data source: Reactome
xref: UnificationXref49
xref: UnificationXref48
cellular location: plasma membrane
comment: Reactome DB_ID: 195729

SequenceSite3
type: SequenceSite
position status: EQUAL
sequence position: 2

SequenceSite4
type: SequenceSite
position status: EQUAL
sequence position: 1097

http://identifiers.org/pubmed/10508393
type: PublicationXref
id: 10508393
author: Kochendoerfer, GG
author: DeGrado, WF
author: Kent, SB
author: Wilk-Orescan, R
author: Salom, D
author: Lear, JD
db: Pubmed
title: Total chemical synthesis of the integral membrane protein influenza A virus M2: role of its C-terminal domain in tetramer assembly
source: Biochemistry 38:11905-13
year: 1999

http://identifiers.org/pubmed/3023071
type: PublicationXref
id: 3023071
author: Jones, IM
author: Philpott, KL
author: Reay, PA
db: Pubmed
title: Nuclear location of all three influenza polymerase proteins and a nuclear signal in polymerase PB2
source: EMBO J 5:2371-6
year: 1986

SequenceSite5
type: SequenceSite
position status: EQUAL
sequence position: 2

SequenceSite6
type: SequenceSite
position status: EQUAL
sequence position: 641

UnificationXref9
type: UnificationXref
id: REACT_10385
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10385.2

UnificationXref8
type: UnificationXref
id: 195764
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195764

UnificationXref7
type: UnificationXref
id: REACT_10176
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10176.2

UnificationXref6
type: UnificationXref
id: 195724
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195724

UnificationXref5
type: UnificationXref
id: REACT_10734
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10734.3

UnificationXref96
type: UnificationXref
id: 196480
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=196480

UnificationXref97
type: UnificationXref
id: REACT_10306
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10306.3

UnificationXref94
type: UnificationXref
id: REACT_10406
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10406.3

UnificationXref95
type: UnificationXref
id: AF389115
db: EMBL

UnificationXref98
type: UnificationXref
id: 195925
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195925

http://identifiers.org/pubmed/10890900
type: PublicationXref
id: 10890900
author: Somerharju, P
author: Ehnholm, C
author: Lusa, S
author: Ikonen, E
author: Olkkonen, VM
author: Heino, S
db: Pubmed
title: Dissecting the role of the golgi complex and lipid rafts in biosynthetic transport of cholesterol to the cell surface
source: Proc Natl Acad Sci U S A 97:8375-80
year: 2000

UnificationXref99
type: UnificationXref
id: REACT_10426
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10426.2

http://identifiers.org/pubmed/15308710
type: PublicationXref
id: 15308710
author: Fodor, E
author: Smith, M
db: Pubmed
title: The PA subunit is required for efficient nuclear accumulation of the PB1 subunit of the influenza A virus RNA polymerase complex
source: J Virol 78:9144-53
year: 2004

UniProt:P03433 PA
type: ProteinReference
name: PA
xref: UnificationXref69
comment: FUNCTION Implicated in endonuclease cleavage of capped RNA primers. Displays an elongation factor activity in viral RNA synthesis. Dispensable for viral transcription, but not replication.SUBUNIT Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.SIMILARITY Belongs to the influenza viruses PA family.
organism: Influenza A virus

UnificationXref3
type: UnificationXref
id: P03468
db: UniProt

UnificationXref4
type: UnificationXref
id: 195751
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195751

UnificationXref1
type: UnificationXref
id: GO:0000139
db: GENE ONTOLOGY

UnificationXref2
type: UnificationXref
id: 11320
db: NCBI Taxonomy

PB1
type: ProteinReference
name: UniProt:P03431 PB1
xref: UnificationXref58
comment: FUNCTION RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription.CATALYTIC ACTIVITY Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SUBUNIT Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.SIMILARITY Belongs to the influenza viruses polymerase PB1 family.SIMILARITY Contains 1 RdRp catalytic domain.
organism: Influenza A virus

UnificationXref159
type: UnificationXref
id: GO:0005789
db: GENE ONTOLOGY

UnificationXref87
type: UnificationXref
id: 196477
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=196477

UnificationXref88
type: UnificationXref
id: REACT_10987
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10987.3

UnificationXref89
type: UnificationXref
id: AF389119
db: EMBL

UnificationXref83
type: UnificationXref
id: AF389116
db: EMBL

UnificationXref84
type: UnificationXref
id: 196492
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=196492

UnificationXref85
type: UnificationXref
id: REACT_10334
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10334.3

UnificationXref86
type: UnificationXref
id: AF389121
db: EMBL

UnificationXref91
type: UnificationXref
id: REACT_10499
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10499.3

UnificationXref90
type: UnificationXref
id: 196499
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=196499

UnificationXref93
type: UnificationXref
id: 196489
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=196489

UnificationXref150
type: UnificationXref
id: 192906
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=192906

UnificationXref92
type: UnificationXref
id: AF389117
db: EMBL

cytosol
type: CellularLocationVocabulary
xref: UnificationXref54

UniProt:P03468 NA
type: ProteinReference
name: NA
xref: UnificationXref3
comment: FUNCTION Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.CATALYTIC ACTIVITY Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.ENZYME REGULATION Inhibited by the neuraminidase inhibitors zanamivir (Relenza) and oseltamivir (Tamiflu). These drugs interfere with the release of progeny virus from infected cells and are effective against all influenza strains. Resistance to neuraminidase inhibitors is quite rare.MISCELLANEOUS The influenza A genome consist of 8 RNA segments. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. The mechanism of variation can be the result of point mutations or the result of genetic reassortment between segments of two different strains.SIMILARITY Belongs to the glycosyl hydrolase 34 fa…
organism: Influenza A virus

UniProt:P03466 NP
type: ProteinReference
name: NP
xref: UnificationXref12
comment: FUNCTION Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.SUBUNIT Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases.SIMILARITY Belongs to the influenza viruses nucleoprotein family.
organism: Influenza A virus

UnificationXref155
type: UnificationXref
id: REACT_9918
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9918.1

UnificationXref156
type: UnificationXref
id: P08107
db: UniProt

UnificationXref157
type: UnificationXref
id: 192715
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=192715

UnificationXref158
type: UnificationXref
id: REACT_9660
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9660.3

UnificationXref151
type: UnificationXref
id: REACT_9769
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9769.1

UnificationXref152
type: UnificationXref
id: 192746
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=192746

UnificationXref153
type: UnificationXref
id: REACT_9407
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9407.3

UnificationXref154
type: UnificationXref
id: 192889
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=192889

UnificationXref78
type: UnificationXref
id: 196496
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=196496

UnificationXref79
type: UnificationXref
id: REACT_11005
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_11005.1

UnificationXref76
type: UnificationXref
id: 188874
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188874

UnificationXref77
type: UnificationXref
id: REACT_9345
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9345.3

UnificationXref74
type: UnificationXref
id: REACT_9746
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9746.3

Reactome
type: Provenance
comment: http://www.reactome.org

UnificationXref75
type: UnificationXref
id: AF389122
db: EMBL

UnificationXref72
type: UnificationXref
id: P03508
db: UniProt

UnificationXref73
type: UnificationXref
id: 192845
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=192845

UnificationXref160
type: UnificationXref
id: 195756
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195756

UnificationXref82
type: UnificationXref
id: REACT_10243
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10243.3

UnificationXref161
type: UnificationXref
id: REACT_10359
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10359.3

UnificationXref81
type: UnificationXref
id: 196479
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=196479

UnificationXref80
type: UnificationXref
id: AF389120
db: EMBL

UniProt:P06821 M
type: ProteinReference
name: M
xref: UnificationXref17
comment: ENZYME REGULATION The M2 protein from most influenza A strains is inhibited by amantadine and rimantadine, resulting in viral uncoating incapacity. Emergence of amantadine-resistant variants is usually rapid.MISCELLANEOUS When the channel is activated, one or more imidazole moities of His-37 probably become bi-protonated.SIMILARITY Belongs to the influenza viruses matrix protein M2 family.
organism: Influenza A virus

UniProt:P03452 HA
type: ProteinReference
name: HA
xref: UnificationXref22
comment: FUNCTION Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.SUBUNIT Homotrimer of disulfide-linked HA1-HA2.MISCELLANEOUS Major glycoprotein, comprises over 80% of the envelope proteins present in virus particle.MISCELLANEOUS The extent of infection into host organism is determined by HA. Influenza viruses bud from the apical surface of polarized epithelial cells (e.g. bronchial epithelial cells) into lumen of lungs and are therefore usually pneumotropic. The reason is that HA is cleaved by tryptase clara which is restricted to lungs. However, HAs of H5 and H7 pantropic avian viruses subtypes can be cleaved by furin and subtilisin-type enzymes, allowing the virus to grow in other organs than lungs.MISCELLANEOUS The influenza A genome consist of 8 RNA segments. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. The mechanism of variation can be the result of point mutations or the result of genetic reassortment between segments of two different strains.SIMILARITY Belongs to the influenza viruses hemagglutinin family.
organism: Influenza A virus

UnificationXref164
type: UnificationXref
id: 188544
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188544

UnificationXref165
type: UnificationXref
id: REACT_10123
db: Reactome
id version: 4
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10123.4

UnificationXref162
type: UnificationXref
id: 195797
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195797

UnificationXref163
type: UnificationXref
id: REACT_10501
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10501.2

UnificationXref132
type: UnificationXref
id: 188867
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188867

UnificationXref131
type: UnificationXref
id: REACT_9332
db: Reactome
id version: 5
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9332.5

UnificationXref130
type: UnificationXref
id: 188826
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188826

Ran binding protein-5
type: Protein
entity reference: RANBP5
data source: Reactome
name: Importin beta-3
xref: UnificationXref122
xref: UnificationXref123
feature: FragmentFeature2
cellular location: nucleoplasm
comment: Reactome DB_ID: 192675
display name: IPO5

UnificationXref136
type: UnificationXref
id: 188868
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188868

M
type: ProteinReference
name: UniProt:P03485 M
xref: UnificationXref61
comment: FUNCTION Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place.FUNCTION Determines the virion's shape: spherical or filamentous. Clinical isolates of influenza are characterized by the presence of significant proportion of filamentous virions, whereas after multiple passage on eggs or cell culture, virions have only spherical morphology. Filamentous virions are thought to be important to infect neighboring cells, and spherical virions more suited to spread through aerosol between hosts organisms.SUBUNIT Homodimer and homomultimer. Interacts with NEP. Binds ribonucleocapsid by both interacting with genomic RNA and NP protein. May interact with HA and NA (By similarity). Cannot bind NP without genomic RNA.MISCELLANEOUS Most abundant protein in virion. When expressed alone can form virus-like particles in transfected cells.SIMILARITY Belongs to the influenza viruses Matrix protein M1 family.
organism: Influenza A virus

UnificationXref135
type: UnificationXref
id: REACT_9081
db: Reactome
id version: 5
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9081.5

UnificationXref134
type: UnificationXref
id: 188858
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188858

UnificationXref133
type: UnificationXref
id: REACT_9356
db: Reactome
id version: 5
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9356.5

UnificationXref70
type: UnificationXref
id: 195948
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195948

UnificationXref71
type: UnificationXref
id: REACT_10459
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10459.2

UnificationXref64
type: UnificationXref
id: AF389118
db: EMBL

UnificationXref63
type: UnificationXref
id: REACT_9872
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9872.2

UnificationXref62
type: UnificationXref
id: 169073
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=169073

UnificationXref61
type: UnificationXref
id: P03485
db: UniProt

UnificationXref68
type: UnificationXref
id: REACT_9256
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9256.2

PB1
type: Protein
entity reference: PB1
data source: Reactome
xref: UnificationXref59
xref: UnificationXref60
cellular location: cytosol
comment: Reactome DB_ID: 195940

UnificationXref67
type: UnificationXref
id: 188848
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188848

UnificationXref66
type: UnificationXref
id: REACT_9137
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9137.3

UnificationXref65
type: UnificationXref
id: 188856
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188856

Stoichiometry64
type: Stoichiometry
physical entity: M2
stoichiometric coefficient: 4.0

UnificationXref139
type: UnificationXref
id: REACT_9174
db: Reactome
id version: 5
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9174.5

PA
type: Protein
entity reference: UniProt:P03433 PA
data source: Reactome
xref: UnificationXref70
xref: UnificationXref71
cellular location: cytosol
comment: Reactome DB_ID: 195948

Stoichiometry65
type: Stoichiometry
physical entity: M2
stoichiometric coefficient: 4.0

NEP/NS2
type: Protein
entity reference: UniProt:P03508 NS
data source: Reactome
xref: UnificationXref73
xref: UnificationXref74
cellular location: cytosol
comment: Reactome DB_ID: 192845

UnificationXref137
type: UnificationXref
id: REACT_9195
db: Reactome
id version: 5
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9195.5

M1
type: Protein
entity reference: M
data source: Reactome
xref: UnificationXref62
xref: UnificationXref63
cellular location: cytosol
comment: Reactome DB_ID: 169073

UnificationXref138
type: UnificationXref
id: 188863
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188863

NP
type: Protein
entity reference: UniProt:P03466 NP
data source: Reactome
xref: UnificationXref68
xref: UnificationXref67
cellular location: cytosol
comment: Reactome DB_ID: 188848

UnificationXref69
type: UnificationXref
id: P03433
db: UniProt

PB1
type: Protein
entity reference: PB1
data source: Reactome
xref: UnificationXref108
xref: UnificationXref107
cellular location: nucleoplasm
comment: Reactome DB_ID: 188843

HSP90AA1
type: Protein
entity reference: HSP90AA1
data source: Reactome
xref: UnificationXref118
xref: UnificationXref117
feature: FragmentFeature1
cellular location: cytosol
comment: Reactome DB_ID: 192864
display name: HSP90

PA
type: Protein
entity reference: UniProt:P03433 PA
data source: Reactome
xref: UnificationXref103
xref: UnificationXref104
cellular location: nucleoplasm
comment: Reactome DB_ID: 188877

PB2
type: Protein
entity reference: PB2
data source: Reactome
xref: UnificationXref105
xref: UnificationXref106
cellular location: nucleoplasm
comment: Reactome DB_ID: 188857

UnificationXref141
type: UnificationXref
id: REACT_9171
db: Reactome
id version: 5
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9171.5

UnificationXref140
type: UnificationXref
id: 188839
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188839

UnificationXref143
type: UnificationXref
id: REACT_9646
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9646.2

UnificationXref142
type: UnificationXref
id: 192849
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=192849

Stoichiometry63
type: Stoichiometry
physical entity: M1
stoichiometric coefficient: 1.0

UnificationXref145
type: UnificationXref
id: REACT_9729
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9729.2

Stoichiometry62
type: Stoichiometry
physical entity: vRNP destined for Export
stoichiometric coefficient: 1.0

UnificationXref144
type: UnificationXref
id: 193302
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=193302

UnificationXref147
type: UnificationXref
id: REACT_9574
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9574.2

Stoichiometry61
type: Stoichiometry
physical entity: PB1
stoichiometric coefficient: 1.0

UnificationXref146
type: UnificationXref
id: 192922
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=192922

Stoichiometry60
type: Stoichiometry
physical entity: PB2
stoichiometric coefficient: 1.0

http://identifiers.org/pubmed/10644350
type: PublicationXref
id: 10644350
author: Helenius, A
author: Whittaker, GR
author: Wills, EG
author: Bui, M
db: Pubmed
title: Role of the influenza virus M1 protein in nuclear export of viral ribonucleoproteins
source: J Virol 74:1781-6
year: 2000

UnificationXref60
type: UnificationXref
id: REACT_10363
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10363.2

UnificationXref51
type: UnificationXref
id: REACT_10389
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10389.1

UnificationXref50
type: UnificationXref
id: 195732
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195732

UnificationXref53
type: UnificationXref
id: REACT_10039
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10039.3

UnificationXref52
type: UnificationXref
id: 195730
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195730

Glycosylated, palmitylated and folded HA trimer:Lipid Raft Complex
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry17
component stoichiometry: Stoichiometry15
component: Glycosylated, palmitylated and folded HA trimer
component: Lipid Raft
xref: UnificationXref46
xref: UnificationXref47
cellular location: plasma membrane
comment: Reactome DB_ID: 195755

UnificationXref55
type: UnificationXref
id: P03428
db: UniProt

Glycosylated, palmitylated and folded HA trimer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry16
component: HA folded, glycosylated, and palmitylated
xref: UnificationXref44
xref: UnificationXref45
cellular location: plasma membrane
comment: Reactome DB_ID: 195738

UnificationXref54
type: UnificationXref
id: GO:0005829
db: GENE ONTOLOGY

palmitylated M2 Tetramer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry18
component: palmitylated M2
xref: UnificationXref50
xref: UnificationXref51
cellular location: plasma membrane
comment: Reactome DB_ID: 195732

UnificationXref57
type: UnificationXref
id: REACT_10186
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10186.2

UnificationXref56
type: UnificationXref
id: 195946
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195946

RNP pre-assembly complex
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry30
component stoichiometry: Stoichiometry26
component stoichiometry: Stoichiometry27
component stoichiometry: Stoichiometry25
component stoichiometry: Stoichiometry31
component stoichiometry: Stoichiometry29
component stoichiometry: Stoichiometry28
component stoichiometry: Stoichiometry24
component: Genomic RNA Segment 4
component: PB2
component: PA
component: M1
component: NP
component: PB1
component: NEP/NS2
component: Genomic RNA Segment 8
xref: UnificationXref79
xref: UnificationXref78
cellular location: cytosol
comment: Reactome DB_ID: 196496

UnificationXref148
type: UnificationXref
id: 186959
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=186959

Stoichiometry55
type: Stoichiometry
physical entity: PB1
stoichiometric coefficient: 1.0

UnificationXref59
type: UnificationXref
id: 195940
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195940

Matrix associated RNP pre-assembly complex
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry49
component stoichiometry: Stoichiometry51
component stoichiometry: Stoichiometry48
component stoichiometry: Stoichiometry46
component stoichiometry: Stoichiometry38
component stoichiometry: Stoichiometry39
component stoichiometry: Stoichiometry42
component stoichiometry: Stoichiometry43
component stoichiometry: Stoichiometry36
component stoichiometry: Stoichiometry44
component stoichiometry: Stoichiometry47
component stoichiometry: Stoichiometry52
component stoichiometry: Stoichiometry41
component stoichiometry: Stoichiometry37
component stoichiometry: Stoichiometry40
component stoichiometry: Stoichiometry45
component stoichiometry: Stoichiometry50
component: Gycosylated NA Tetramer
component: Glycosylated, palmitylated and folded HA trimer
component: Genomic RNA Segment 3
component: Genomic RNA Segment 6
component: PB2
component: Genomic RNA Segment 1
component: M1
component: Genomic RNA Segment 5
component: Genomic RNA Segment 4
component: PA
component: PB1
component: Genomic RNA Segment 7
component: palmitylated M2 Tetramer
component: NEP/NS2
component: Genomic RNA Segment 2
component: Genomic RNA Segment 8
component: NP
name: Ribonucleoprotein (RNP) Complex
name: matrix associated complete RNP complex
xref: UnificationXref98
xref: UnificationXref99
cellular location: plasma membrane
display name: Intracellular assembly complex
comment: Reactome DB_ID: 195925

UnificationXref149
type: UnificationXref
id: REACT_9764
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9764.2

Stoichiometry56
type: Stoichiometry
physical entity: NP
stoichiometric coefficient: 3.0

Viral RNA dependent RNA polymerase
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry55
component stoichiometry: Stoichiometry54
component stoichiometry: Stoichiometry53
component: PA
component: PB2
component: PB1
xref: UnificationXref109
xref: UnificationXref110
cellular location: nucleoplasm
display name: Viral Polymerase
comment: Reactome DB_ID: 192720

UnificationXref58
type: UnificationXref
id: P03431
db: UniProt

vRNP destined for Export
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry61
component stoichiometry: Stoichiometry58
component stoichiometry: Stoichiometry60
component stoichiometry: Stoichiometry59
component: PA
component: PB2
component: vRNA (Genomic):NP Complex
component: PB1
xref: UnificationXref147
xref: UnificationXref146
cellular location: nucleoplasm
comment: Reactome DB_ID: 192922

Stoichiometry53
type: Stoichiometry
physical entity: PA
stoichiometric coefficient: 1.0

vRNA (Genomic):NP Complex
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry57
component stoichiometry: Stoichiometry56
component: vRNA (genomic)
component: NP
xref: UnificationXref145
xref: UnificationXref144
cellular location: nucleoplasm
comment: Reactome DB_ID: 193302

Stoichiometry54
type: Stoichiometry
physical entity: PB2
stoichiometric coefficient: 1.0

vRNP:M1 for Export
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry62
component stoichiometry: Stoichiometry63
component: M1
component: vRNP destined for Export
xref: UnificationXref151
xref: UnificationXref150
cellular location: nucleoplasm
comment: Reactome DB_ID: 192906

Stoichiometry59
type: Stoichiometry
physical entity: PA
stoichiometric coefficient: 1.0

M2 Tetramer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry65
component: M2
xref: UnificationXref163
xref: UnificationXref162
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 195797

Stoichiometry57
type: Stoichiometry
physical entity: vRNA (genomic)
stoichiometric coefficient: 1.0

Stoichiometry58
type: Stoichiometry
physical entity: vRNA (Genomic):NP Complex
stoichiometric coefficient: 1.0

UnificationXref114
type: UnificationXref
id: REACT_9915
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9915.1

Stoichiometry50
type: Stoichiometry
physical entity: palmitylated M2 Tetramer
stoichiometric coefficient: 60.0

http://identifiers.org/pubmed/14722281
type: PublicationXref
id: 14722281
author: Hirayama, E
author: Kim, J
author: Hiraki, A
author: Atagi, H
db: Pubmed
title: Heat shock protein 70 is related to thermal inhibition of nuclear export of the influenza virus ribonucleoprotein complex
source: J Virol 78:1263-70
year: 2004

UnificationXref113
type: UnificationXref
id: 192953
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=192953

UnificationXref112
type: UnificationXref
id: REACT_9428
db: Reactome
id version: 4
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9428.4

Stoichiometry52
type: Stoichiometry
physical entity: Genomic RNA Segment 1
stoichiometric coefficient: 1.0

UnificationXref111
type: UnificationXref
id: 192830
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=192830

Stoichiometry51
type: Stoichiometry
physical entity: Genomic RNA Segment 8
stoichiometric coefficient: 1.0

UnificationXref110
type: UnificationXref
id: REACT_9586
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9586.1

UnificationXref46
type: UnificationXref
id: 195755
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195755

UnificationXref45
type: UnificationXref
id: REACT_10978
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10978.1

UnificationXref44
type: UnificationXref
id: 195738
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195738

UnificationXref43
type: UnificationXref
id: REACT_10939
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10939.2

UnificationXref42
type: UnificationXref
id: 195762
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195762

UnificationXref41
type: UnificationXref
id: REACT_10662
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10662.1

UnificationXref40
type: UnificationXref
id: 195735
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195735

Stoichiometry46
type: Stoichiometry
physical entity: Genomic RNA Segment 5
stoichiometric coefficient: 1.0

Stoichiometry47
type: Stoichiometry
physical entity: Genomic RNA Segment 3
stoichiometric coefficient: 1.0

UnificationXref119
type: UnificationXref
id: 192952
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=192952

Stoichiometry48
type: Stoichiometry
physical entity: Glycosylated, palmitylated and folded HA trimer
stoichiometric coefficient: 500.0

Stoichiometry49
type: Stoichiometry
physical entity: PA
stoichiometric coefficient: 45.0

UnificationXref117
type: UnificationXref
id: 192864
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=192864

Stoichiometry42
type: Stoichiometry
physical entity: Genomic RNA Segment 2
stoichiometric coefficient: 1.0

UnificationXref118
type: UnificationXref
id: REACT_9878
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9878.3

UnificationXref49
type: UnificationXref
id: REACT_10694
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10694.2

Stoichiometry43
type: Stoichiometry
physical entity: PB1
stoichiometric coefficient: 45.0

UnificationXref115
type: UnificationXref
id: 9606
db: NCBI Taxonomy

UnificationXref48
type: UnificationXref
id: 195729
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195729

Stoichiometry44
type: Stoichiometry
physical entity: PB2
stoichiometric coefficient: 45.0

UnificationXref116
type: UnificationXref
id: P07900
db: UniProt

UnificationXref47
type: UnificationXref
id: REACT_10581
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10581.1

Stoichiometry45
type: Stoichiometry
physical entity: Genomic RNA Segment 7
stoichiometric coefficient: 1.0

UnificationXref123
type: UnificationXref
id: REACT_9535
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9535.2

Stoichiometry41
type: Stoichiometry
physical entity: NEP/NS2
stoichiometric coefficient: 165.0

UnificationXref122
type: UnificationXref
id: 192675
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=192675

Stoichiometry40
type: Stoichiometry
physical entity: Genomic RNA Segment 6
stoichiometric coefficient: 1.0

nucleoplasm
type: CellularLocationVocabulary
xref: UnificationXref102

http://identifiers.org/pubmed/15298170
type: PublicationXref
id: 15298170
author: Schmitt, AP
author: Lamb, RA
db: Pubmed
title: Escaping from the cell: assembly and budding of negative-strand RNA viruses
source: Curr Top Microbiol Immunol 283:145-96
year: 2004

UnificationXref125
type: UnificationXref
id: REACT_9117
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9117.2

UnificationXref124
type: UnificationXref
id: 188832
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188832

http://identifiers.org/pubmed/8948635
type: PublicationXref
id: 8948635
author: Gonzalez, S
author: Zurcher, T
author: Ortin, J
db: Pubmed
title: Identification of two separate domains in the influenza virus PB1 protein involved in the interaction with the PB2 and PA subunits: a model for the viral RNA polymerase structure
source: Nucleic Acids Res 24:4456-63
year: 1996

UnificationXref121
type: UnificationXref
id: O00410
db: UniProt

UnificationXref120
type: UnificationXref
id: REACT_9924
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9924.1

Stoichiometry1
type: Stoichiometry
physical entity: Glycosylated NA
stoichiometric coefficient: 4.0

http://identifiers.org/pubmed/3783823
type: PublicationXref
id: 3783823
author: St, Angelo C
author: Detjen, BM
author: Krug, RM
author: Katze, MG
db: Pubmed
title: The three influenza virus polymerase (P) proteins not associated with viral nucleocapsids in the infected cell are in the form of a complex
source: J Virol 61:16-22
year: 1987

UnificationXref33
type: UnificationXref
id: REACT_10966
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10966.2

Stoichiometry6
type: Stoichiometry
physical entity: palmitylated M2
stoichiometric coefficient: 4.0

UnificationXref32
type: UnificationXref
id: 195752
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195752

Stoichiometry7
type: Stoichiometry
physical entity: HA folded, glycosylated, and palmitylated
stoichiometric coefficient: 3.0

UnificationXref35
type: UnificationXref
id: REACT_10830
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10830.1

Stoichiometry8
type: Stoichiometry
physical entity: Glycosylated, palmitylated and folded HA trimer
stoichiometric coefficient: 1.0

http://identifiers.org/pubmed/10438836
type: PublicationXref
id: 10438836
author: McInnis, J
author: Ye, Z
author: Liu, T
author: Levandowski, RA
author: Offringa, DP
db: Pubmed
title: Association of influenza virus matrix protein with ribonucleoproteins
source: J Virol 73:7467-73
year: 1999

UnificationXref34
type: UnificationXref
id: 195766
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195766

Stoichiometry9
type: Stoichiometry
physical entity: Lipid Raft
stoichiometric coefficient: 1.0

Stoichiometry2
type: Stoichiometry
physical entity: Glycosylated NA Tetramer
stoichiometric coefficient: 1.0

Stoichiometry3
type: Stoichiometry
physical entity: Lipid Raft
stoichiometric coefficient: 1.0

UnificationXref31
type: UnificationXref
id: REACT_10332
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10332.1

Stoichiometry4
type: Stoichiometry
physical entity: Lipid Raft
stoichiometric coefficient: 1.0

Stoichiometry39
type: Stoichiometry
physical entity: M1
stoichiometric coefficient: 3000.0

UnificationXref30
type: UnificationXref
id: 195731
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195731

Stoichiometry5
type: Stoichiometry
physical entity: NP
stoichiometric coefficient: 1.0

Stoichiometry37
type: Stoichiometry
physical entity: Gycosylated NA Tetramer
stoichiometric coefficient: 100.0

Stoichiometry38
type: Stoichiometry
physical entity: NP
stoichiometric coefficient: 1000.0

Stoichiometry35
type: Stoichiometry
physical entity: palmitylated M2 Tetramer
stoichiometric coefficient: 60.0

Stoichiometry36
type: Stoichiometry
physical entity: Genomic RNA Segment 4
stoichiometric coefficient: 1.0

UnificationXref126
type: UnificationXref
id: 188875
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188875

UnificationXref37
type: UnificationXref
id: REACT_10884
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10884.2

Stoichiometry33
type: Stoichiometry
physical entity: PA
stoichiometric coefficient: 37.0

UnificationXref127
type: UnificationXref
id: REACT_9275
db: Reactome
id version: 5
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9275.5

UnificationXref36
type: UnificationXref
id: 195750
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195750

Stoichiometry34
type: Stoichiometry
physical entity: NEP/NS2
stoichiometric coefficient: 157.0

UnificationXref128
type: UnificationXref
id: 188827
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188827

UnificationXref39
type: UnificationXref
id: REACT_10841
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10841.1

Stoichiometry31
type: Stoichiometry
physical entity: Genomic RNA Segment 8
stoichiometric coefficient: 1.0

UnificationXref129
type: UnificationXref
id: REACT_9251
db: Reactome
id version: 5
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9251.5

UnificationXref38
type: UnificationXref
id: 195728
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195728

Stoichiometry32
type: Stoichiometry
physical entity: Glycosylated, palmitylated and folded HA trimer
stoichiometric coefficient: 500.0

RANBP5
type: ProteinReference
name: IPO5
name: UniProt:O00410 IPO5
name: KPNB3
xref: UnificationXref121
comment: FUNCTION Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.SUBUNIT Binds RPL23A, RPS7 and RPL5. Interacts with H2A, H2B, H3 and H4 histones (By similarity). Binds to HIV-1 Rev.SIMILARITY Belongs to the importin beta family.SIMILARITY Contains 6 HEAT repeats.SIMILARITY Contains 1 importin N-terminal domain.
organism: Homo sapiens

HSP90AA1
type: ProteinReference
name: HSPCA
name: HSPC1
name: HSP90A
name: UniProt:P07900 HSP90AA1
xref: UnificationXref116
comment: FUNCTION Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.SUBUNIT Homodimer. Interacts with AHSA1, FNIP1, HSF1, SMYD3 and TOM34. Interacts with TERT; the interaction, together with PTGES3, is required for correct assembly and stabilization of the TERT holoenzyme complex. Interacts with CHORDC1 and DNAJC7. Interacts with STUB1 and UBE2N; may couple the chaperone and ubiquitination systems.DOMAIN The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.PTM ISGylated.PTM S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.SIMILARITY Belongs to the heat shock protein 90 family.
organism: Homo sapiens

endoplasmic reticulum membrane
type: CellularLocationVocabulary
xref: UnificationXref159

NP:Lipid Raft
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry5
component stoichiometry: Stoichiometry4
component: NP
component: Lipid Raft
xref: UnificationXref15
xref: UnificationXref16
cellular location: Golgi membrane
comment: Reactome DB_ID: 195737

palmitylated M2 Tetramer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry6
component: palmitylated M2
xref: UnificationXref21
xref: UnificationXref20
cellular location: Golgi membrane
comment: Reactome DB_ID: 195761

Gycosylated NA Tetramer:Lipid Raft
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry3
component stoichiometry: Stoichiometry2
component: Glycosylated NA Tetramer
component: Lipid Raft
xref: UnificationXref11
xref: UnificationXref10
cellular location: Golgi membrane
comment: Reactome DB_ID: 195736

Glycosylated NA Tetramer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry1
component: Glycosylated NA
xref: UnificationXref7
xref: UnificationXref6
cellular location: Golgi membrane
comment: Reactome DB_ID: 195724

UnificationXref27
type: UnificationXref
id: 195723
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195723

UnificationXref28
type: UnificationXref
id: REACT_10369
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10369.2

http://identifiers.org/pubmed/17005651
type: PublicationXref
id: 17005651
author: Deng, T
author: Fodor, E
author: Brownlee, GG
author: Thomas, B
author: Akoulitchev, AV
author: Engelhardt, OG
db: Pubmed
title: Role of ran binding protein 5 in nuclear import and assembly of the influenza virus RNA polymerase complex
source: J Virol 80:11911-9
year: 2006

UnificationXref25
type: UnificationXref
id: 195725
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195725

UnificationXref26
type: UnificationXref
id: REACT_10197
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10197.2

UnificationXref29
type: UnificationXref
id: GO:0005886
db: GENE ONTOLOGY

UnificationXref20
type: UnificationXref
id: 195761
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195761

UnificationXref23
type: UnificationXref
id: 195743
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195743

UnificationXref24
type: UnificationXref
id: REACT_11023
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_11023.3

UnificationXref21
type: UnificationXref
id: REACT_10582
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10582.2

UnificationXref22
type: UnificationXref
id: P03452
db: UniProt

UnificationXref100
type: UnificationXref
id: 195926
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195926

UnificationXref101
type: UnificationXref
id: REACT_10077
db: Reactome
id version: 4
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10077.4

UnificationXref102
type: UnificationXref
id: GO:0005654
db: GENE ONTOLOGY

UnificationXref103
type: UnificationXref
id: 188877
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188877

Golgi membrane
type: CellularLocationVocabulary
xref: UnificationXref1

UnificationXref105
type: UnificationXref
id: 188857
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188857

UnificationXref14
type: UnificationXref
id: REACT_10689
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10689.3

Glycosylated, palmitylated and folded HA trimer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry7
component: HA folded, glycosylated, and palmitylated
xref: UnificationXref26
xref: UnificationXref25
cellular location: Golgi membrane
comment: Reactome DB_ID: 195725

UnificationXref104
type: UnificationXref
id: REACT_9134
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9134.2

UnificationXref15
type: UnificationXref
id: 195737
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195737

Glycosylated, palmitylated and folded HA trimer:Lipid Raft Complex
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry8
component stoichiometry: Stoichiometry9
component: Glycosylated, palmitylated and folded HA trimer
component: Lipid Raft
xref: UnificationXref28
xref: UnificationXref27
cellular location: Golgi membrane
comment: Reactome DB_ID: 195723

UnificationXref107
type: UnificationXref
id: 188843
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=188843

Gycosylated NA Tetramer:Lipid Raft
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry12
component stoichiometry: Stoichiometry14
component: Gycosylated NA Tetramer
component: Lipid Raft
xref: UnificationXref40
xref: UnificationXref41
cellular location: plasma membrane
comment: Reactome DB_ID: 195735

UnificationXref16
type: UnificationXref
id: REACT_10315
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10315.2

UnificationXref106
type: UnificationXref
id: REACT_9083
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9083.2

NP:Lipid Raft
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry11
component stoichiometry: Stoichiometry10
component: Lipid Raft
component: NP
xref: UnificationXref35
xref: UnificationXref34
cellular location: plasma membrane
comment: Reactome DB_ID: 195766

UnificationXref17
type: UnificationXref
id: P06821
db: UniProt

UnificationXref109
type: UnificationXref
id: 192720
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=192720

UnificationXref18
type: UnificationXref
id: 195741
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195741

</gene>
type: ProteinReference
name: <gene>
name: HSPA1
name: UniProt:P08107 HSPA1A
name: HSPA1A
name: HSPA1B
xref: UnificationXref156
comment: FUNCTION In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.SUBUNIT Component of the CatSper complex (By similarity). Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with TSC2. Interacts with IRAK1BP1. Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed. Interacts with DNAJC7. Interacts with CHCHD3. Interacts with TRIM5 (via B30.2/SPRY domain).TISSUE SPECIFICITY HSPA1B is testis-specific.INDUCTION By heat shock.SIMILARITY Belongs to the heat shock protein 70 family.
organism: Homo sapiens

UnificationXref108
type: UnificationXref
id: REACT_9265
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9265.2

Gycosylated NA Tetramer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry13
component: Glycosylated NA
xref: UnificationXref38
xref: UnificationXref39
cellular location: plasma membrane
comment: Reactome DB_ID: 195728

UnificationXref19
type: UnificationXref
id: REACT_10510
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10510.3

UnificationXref10
type: UnificationXref
id: 195736
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195736

UnificationXref11
type: UnificationXref
id: REACT_10954
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_10954.2

UnificationXref12
type: UnificationXref
id: P03466
db: UniProt

UnificationXref13
type: UnificationXref
id: 195740
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=195740

http://identifiers.org/pubmed/12490406
type: PublicationXref
id: 12490406
author: Muller, J
author: Ye, Z
author: Liu, T
db: Pubmed
title: Association of influenza virus matrix protein with ribonucleoproteins may control viral growth and morphology
source: Virology 304:89-96
year: 2002

Genomic RNA Segment 5
type: Rna
entity reference: H1N1 Genomic RNA Segment 5
data source: Reactome
xref: UnificationXref91
xref: UnificationXref90
cellular location: cytosol
comment: Reactome DB_ID: 196499

Genomic RNA Segment 3
type: Rna
entity reference: EMBL:AF389117 H1N1 Genomic RNA Segment 3
data source: Reactome
xref: UnificationXref94
xref: UnificationXref93
cellular location: cytosol
comment: Reactome DB_ID: 196489

Genomic RNA Segment 1
type: Rna
entity reference: EMBL:AF389115 H1N1 Genomic RNA Segment 1
data source: Reactome
xref: UnificationXref96
xref: UnificationXref97
cellular location: cytosol
comment: Reactome DB_ID: 196480

http://identifiers.org/pubmed/17081640
type: PublicationXref
id: 17081640
author: Ruigrok, RW
author: Baudin, F
author: Boulo, S
author: Akarsu, H
db: Pubmed
title: Nuclear traffic of influenza virus proteins and ribonucleoprotein complexes
source: Virus Res 124:12-21
year: 2006

vRNA (genomic)
type: Rna
data source: Reactome
member physical entity: Genomic RNA Segment 8
member physical entity: Genomic RNA Segment 3
member physical entity: Genomic RNA Segment 7
member physical entity: Genomic RNA Segment 4
member physical entity: Genomic RNA Segment 6
member physical entity: Genomic RNA Segment 1
member physical entity: Genomic RNA Segment 2
member physical entity: Genomic RNA Segment 5
xref: UnificationXref143
xref: UnificationXref142
cellular location: nucleoplasm
comment: Converted from EntitySet in Reactome
comment: Reactome DB_ID: 192849

Genomic RNA Segment 8
type: Rna
entity reference: H1N1 Genomic RNA Segment 8
data source: Reactome
xref: UnificationXref77
xref: UnificationXref76
cellular location: cytosol
comment: Reactome DB_ID: 188874

Genomic RNA Segment 6
type: Rna
entity reference: H1N1 Genomic RNA Segment 6
data source: Reactome
xref: UnificationXref81
xref: UnificationXref82
cellular location: cytosol
comment: Reactome DB_ID: 196479

Genomic RNA Segment 2
type: Rna
entity reference: EMBL:AF389116 H1N1 Genomic RNA Segment 2
data source: Reactome
xref: UnificationXref84
xref: UnificationXref85
cellular location: cytosol
comment: Reactome DB_ID: 196492

Genomic RNA Segment 7
type: Rna
entity reference: H1N1 Genomic RNA Segment 7
data source: Reactome
xref: UnificationXref88
xref: UnificationXref87
cellular location: cytosol
comment: Reactome DB_ID: 196477

UniProt:P03508 NS
type: ProteinReference
name: NS
xref: UnificationXref72
comment: FUNCTION Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced.SUBUNIT Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1.MISCELLANEOUS Average number present in a viral particle is estimated to be 130-200 molecules.SIMILARITY Belongs to the influenza viruses NEP family.
organism: Influenza A virus

Genomic RNA Segment 4
type: Rna
entity reference: H1N1 Genomic RNA Segment 4
data source: Reactome
xref: UnificationXref66
xref: UnificationXref65
cellular location: cytosol
comment: Reactome DB_ID: 188856

http://identifiers.org/pubmed/11531417
type: PublicationXref
id: 11531417
author: Muller, J
author: Ye, Z
author: Liu, T
author: Levandowski, RA
author: Huang, X
db: Pubmed
title: Effect of influenza virus matrix protein and viral RNA on ribonucleoprotein formation and nuclear export
source: Virology 287:405-16
year: 2001

M2
type: Protein
entity reference: UniProt:P06821 M
data source: Reactome
xref: UnificationXref161
xref: UnificationXref160
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 195756

heat shock 70kDa protein 1
type: Protein
entity reference: </gene>
data source: Reactome
name: HSPA1A(2-641)
xref: UnificationXref158
xref: UnificationXref157
feature: FragmentFeature3
cellular location: nucleoplasm
comment: Reactome DB_ID: 192715
display name: HSP70

M1
type: Protein
entity reference: M
data source: Reactome
xref: UnificationXref149
xref: UnificationXref148
cellular location: nucleoplasm
comment: Reactome DB_ID: 186959

NP
type: Protein
entity reference: UniProt:P03466 NP
data source: Reactome
xref: UnificationXref124
xref: UnificationXref125
cellular location: nucleoplasm
comment: Reactome DB_ID: 188832