ubiquitin (UBC 7)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref313
xref: UnificationXref314
feature: FragmentFeature47
cellular location: cytosol
comment: Reactome DB_ID: 939184
display name: UBC(457-532)

ubiquitin (UBC 6)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref312
xref: UnificationXref311
feature: FragmentFeature46
cellular location: cytosol
comment: Reactome DB_ID: 939191
display name: UBC(381-456)

ubiquitin (UBC 9)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref317
xref: UnificationXref318
feature: FragmentFeature49
cellular location: cytosol
comment: Reactome DB_ID: 939165
display name: UBC(609-684)

ubiquitin (UBC 8)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref315
xref: UnificationXref316
feature: FragmentFeature48
cellular location: cytosol
comment: Reactome DB_ID: 939239
display name: UBC(533-608)

ubiquitin (UBC 3)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref305
xref: UnificationXref306
feature: FragmentFeature43
cellular location: cytosol
comment: Reactome DB_ID: 939258
display name: UBC(153-228)

ubiquitin (UBC 5)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref310
xref: UnificationXref309
feature: FragmentFeature45
cellular location: cytosol
comment: Reactome DB_ID: 939232
display name: UBC(305-380)

ubiquitin (UBC 4)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref308
xref: UnificationXref307
feature: FragmentFeature44
cellular location: cytosol
comment: Reactome DB_ID: 939192
display name: UBC(229-304)

ubiquitin
type: Protein
data source: Reactome
member physical entity: ubiquitin (UBC 8)
member physical entity: ubiquitin (UBB 2)
member physical entity: UBB(1-76)
member physical entity: ubiquitin (UBB 3)
member physical entity: ubiquitin (UBC 3)
member physical entity: ubiquitin (UBC 9)
member physical entity: UBA52(1-76)
member physical entity: ubiquitin (UBC 2)
member physical entity: ubiquitin (UBC 7)
member physical entity: UBC(1-76)
member physical entity: ubiquitin (UBC 4)
member physical entity: RPS27A(1-76)
member physical entity: ubiquitin (UBC 5)
member physical entity: ubiquitin (UBC 6)
xref: UnificationXref357
xref: UnificationXref356
cellular location: endosome membrane
comment: Converted from EntitySet in Reactome
comment: Reactome DB_ID: 184258

N-myristoyl GAG (P04591) protein
type: Protein
entity reference: gag
data source: Reactome
xref: UnificationXref327
xref: UnificationXref326
feature: ModificationFeature5
cellular location: endosome membrane
comment: Reactome DB_ID: 184361

plasma membrane
type: CellularLocationVocabulary
xref: UnificationXref54

RPS27A(1-76)
type: Protein
entity reference: UBA80
data source: Reactome
name: ubiquitin (RPS27A)
xref: UnificationXref329
xref: UnificationXref328
feature: FragmentFeature50
cellular location: endosome membrane
comment: Reactome DB_ID: 939238
display name: RPS27A

UniProt:P50750 CDK9
type: ProteinReference
name: CDK9
name: TAK
name: CDC2L4
xref: UnificationXref79
comment: FUNCTION Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement. RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription. AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and N…
organism: Homo sapiens

UBB(1-76)
type: Protein
entity reference: UniProt:P0CG47 UBB
data source: Reactome
name: ubiquitin (UBB 1)
xref: UnificationXref294
xref: UnificationXref295
feature: FragmentFeature38
cellular location: cytosol
comment: Reactome DB_ID: 939214
display name: UBB

UBA52(1-76)
type: Protein
entity reference: UniProt:P62987 UBA52
data source: Reactome
name: ubiquitin (UBA52)
xref: UnificationXref292
xref: UnificationXref291
feature: FragmentFeature37
cellular location: cytosol
comment: Reactome DB_ID: 939203
display name: UBA52

RPS27A(1-76)
type: Protein
entity reference: UBA80
data source: Reactome
name: ubiquitin (RPS27A)
xref: UnificationXref289
xref: UnificationXref288
feature: FragmentFeature36
cellular location: cytosol
comment: Reactome DB_ID: 939205
display name: RPS27A

ubiquitin
type: Protein
data source: Reactome
member physical entity: UBC(1-76)
member physical entity: ubiquitin (UBC 3)
member physical entity: RPS27A(1-76)
member physical entity: ubiquitin (UBC 4)
member physical entity: ubiquitin (UBC 6)
member physical entity: UBA52(1-76)
member physical entity: ubiquitin (UBB 3)
member physical entity: UBB(1-76)
member physical entity: ubiquitin (UBC 8)
member physical entity: ubiquitin (UBC 2)
member physical entity: ubiquitin (UBC 5)
member physical entity: ubiquitin (UBC 7)
member physical entity: ubiquitin (UBB 2)
member physical entity: ubiquitin (UBC 9)
xref: UnificationXref320
xref: UnificationXref319
cellular location: cytosol
comment: Converted from EntitySet in Reactome
comment: Reactome DB_ID: 113595
display name: Ub

N-myristoyltransferase 2
type: Protein
entity reference: NMT2
data source: Reactome
xref: UnificationXref284
xref: UnificationXref283
feature: FragmentFeature35
cellular location: cytosol
comment: Reactome DB_ID: 184377
display name: NMT2

N-myristoyl GAG (P04591) protein
type: Protein
entity reference: gag
data source: Reactome
xref: UnificationXref281
xref: UnificationXref280
feature: ModificationFeature5
cellular location: cytosol
comment: Reactome DB_ID: 184427

ubiquitin (UBC 2)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref303
xref: UnificationXref304
feature: FragmentFeature42
cellular location: cytosol
comment: Reactome DB_ID: 939164
display name: UBC(77-152)

UBC(1-76)
type: Protein
entity reference: UBC
data source: Reactome
name: ubiquitin (UBC 1)
xref: UnificationXref302
xref: UnificationXref301
feature: FragmentFeature41
cellular location: cytosol
comment: Reactome DB_ID: 939188
display name: UBC

ubiquitin (UBB 3)
type: Protein
entity reference: UniProt:P0CG47 UBB
data source: Reactome
xref: UnificationXref299
xref: UnificationXref298
feature: FragmentFeature40
cellular location: cytosol
comment: Reactome DB_ID: 939230
display name: UBB(153-228)

ubiquitin (UBB 2)
type: Protein
entity reference: UniProt:P0CG47 UBB
data source: Reactome
xref: UnificationXref296
xref: UnificationXref297
feature: FragmentFeature39
cellular location: cytosol
comment: Reactome DB_ID: 939213
display name: UBB(77-152)

PML39
type: ProteinReference
name: UniProt:A5D8V6 VPS37C
name: VPS37C
xref: UnificationXref375
comment: FUNCTION Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.SUBUNIT Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoechiometry. Interacts with TSG101, VPS28, MVB12A and MVB12B. Interacts with HGS and STAM2. Interacts with CEP55.PTM Phosphorylated by TBK1.SIMILARITY Belongs to the VPS37 family.SIMILARITY Contains 1 VPS37 C-terminal domain.
organism: Homo sapiens

HMGA1_HUMAN
type: Protein
entity reference: UniProt:P17096 HMGA1
data source: Reactome
name: High mobility group protein HMG-I/HMG-Y
name: HMGI/Y
xref: UnificationXref216
xref: UnificationXref215
feature: FragmentFeature30
cellular location: nucleoplasm
comment: Reactome DB_ID: 3004623
display name: HMGA1

Matrix
type: Protein
data source: Reactome
member physical entity: MA (P04591) protein
member physical entity: MA (P04585) protein
xref: UnificationXref225
xref: UnificationXref226
cellular location: nucleoplasm
comment: Converted from EntitySet in Reactome
comment: Reactome DB_ID: 175256
display name: MA

VIF (P69723) protein
type: Protein
entity reference: vif
data source: Reactome
xref: UnificationXref219
xref: UnificationXref218
cellular location: nucleoplasm
comment: Reactome DB_ID: 175527

MA (P04585) protein
type: Protein
entity reference: gag-pol
data source: Reactome
xref: UnificationXref223
xref: UnificationXref224
feature: FragmentFeature32
cellular location: nucleoplasm
comment: Reactome DB_ID: 3006250

MA (P04591) protein
type: Protein
entity reference: gag
data source: Reactome
xref: UnificationXref222
xref: UnificationXref221
feature: FragmentFeature31
cellular location: nucleoplasm
comment: Reactome DB_ID: 175251

p6 protein
type: Protein
data source: Reactome
member physical entity: p6 (P04585)
member physical entity: p6 (P04591)
xref: UnificationXref234
xref: UnificationXref233
cellular location: nucleoplasm
comment: Converted from EntitySet in Reactome
comment: Reactome DB_ID: 175430

RD
type: ProteinReference
name: RDBP
name: UniProt:P18615 NELFE
name: NELFE
xref: UnificationXref150
comment: FUNCTION Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.SUBUNIT The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE.TISSUE SPECIFICITY Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.DOMAIN The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.PTM Sumoylated.SIMILARITY Belongs to the RRM NELF-E family.SIMILARITY Contains 1 RRM (RNA recognition motif) domain.
organism: Homo sapiens

VPU (P05919) protein
type: Protein
entity reference: UniProt:P05919 vpu
data source: Reactome
xref: UnificationXref227
xref: UnificationXref228
cellular location: nucleoplasm
comment: Reactome DB_ID: 175200

Membrane binding and targetting of GAG proteins
type: Pathway
data source: Reactome
pathway component: Monoubiquitination of N-myristoyl GAG polyprotein
pathway component: Monoubiquitinated N-myristoyl GAG polyprotein is targeted to the late endosomal vesicle membrane by the ESCRT-I complex
pathway component: Transport of GAG to the Plasma Membrane
pathway component: N-myristoylation of GAG polyprotein by NMT2
xref: http://identifiers.org/pubmed/16683918
xref: http://identifiers.org/pubmed/15473846
xref: RelationshipXref14
xref: UnificationXref419
xref: UnificationXref418
xref: PublicationXref38
pathway order: PathwayStep16
pathway order: PathwayStep18
pathway order: PathwayStep17
pathway order: PathwayStep15
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: One of the mysteries of Gag protein involvement in HIV virion assembly is how the proteins are targeted to the proper membrane for budding. Infectious retroviruses do not bud from all of the available membrane surfaces within an infected cell, but primarily from the plasma membrane, which constitutes a small proportion of the total membrane surface in most cells. In polarized cells, the sites of budding are further restricted to the basolateral membrane.
comment: Authored: Gillespie, ME, 2013-03-06
organism: Homo sapiens

p6 (P04585)
type: Protein
entity reference: gag-pol
data source: Reactome
xref: UnificationXref232
xref: UnificationXref231
feature: FragmentFeature34
cellular location: nucleoplasm
comment: Reactome DB_ID: 175300

p6 (P04591)
type: Protein
entity reference: gag
data source: Reactome
xref: UnificationXref229
xref: UnificationXref230
feature: FragmentFeature33
cellular location: nucleoplasm
comment: Reactome DB_ID: 175152

GAG Polyprotein (P04591)
type: Protein
entity reference: gag
data source: Reactome
xref: UnificationXref269
xref: UnificationXref270
cellular location: cytosol
comment: Reactome DB_ID: 184393

Stoichiometry20
type: Stoichiometry
physical entity: DNA-directed RNA polymerase II 23 kDa polypeptide (EC 2.7.7.6) (RPB25) (XAP4) (RPB5)
stoichiometric coefficient: 1.0

SPT4H1 protein
type: Protein
entity reference: SUPT4H
data source: Reactome
name: Transcription initiation protein SPT4 homolog 1
xref: UnificationXref159
xref: UnificationXref160
feature: FragmentFeature25
cellular location: nucleoplasm
comment: Reactome DB_ID: 64905
display name: SUPT4H1

phospho-RD
type: Protein
entity reference: RD
data source: Reactome
name: p-NELFE
xref: UnificationXref153
xref: UnificationXref152
feature: FragmentFeature24
feature: ModificationFeature3
cellular location: nucleoplasm
comment: Reactome DB_ID: 170698
display name: p-RDBP

Negative elongation factor B
type: Protein
entity reference: KIAA1182
data source: Reactome
name: NELF-B protein
name: Cofactor of BRCA1
name: NELF-B
xref: UnificationXref149
xref: UnificationXref148
feature: FragmentFeature23
cellular location: nucleoplasm
comment: Reactome DB_ID: 112427
display name: NELFB

Negative elongation factor A
type: Protein
entity reference: P/OKcl.15
data source: Reactome
name: P/OKCL.15
name: NELFA(2-528)
name: Wolf-Hirshhorn syndrome candidate 2 protein
name: NELF-A protein
xref: UnificationXref146
xref: UnificationXref145
feature: FragmentFeature22
cellular location: nucleoplasm
comment: Reactome DB_ID: 112426
display name: NELF-A

IN (Integrase) (P04585) protein
type: Protein
entity reference: gag-pol
data source: Reactome
xref: UnificationXref200
xref: UnificationXref199
feature: FragmentFeature28
cellular location: nucleoplasm
comment: Reactome DB_ID: 175288

BAF protein
type: Protein
entity reference: UniProt:O75531 BANF1
data source: Reactome
xref: UnificationXref197
xref: UnificationXref196
feature: FragmentFeature27
cellular location: nucleoplasm
comment: Reactome DB_ID: 175030
display name: BANF1

REV (P04618) protein
type: Protein
entity reference: rev
data source: Reactome
xref: UnificationXref185
xref: UnificationXref186
cellular location: nucleoplasm
comment: Reactome DB_ID: 175380

Transcription elongation factor SPT5
type: Protein
entity reference: SPT5H
data source: Reactome
name: p-SUPT5H
name: SPT5H_HUMAN
xref: UnificationXref162
xref: UnificationXref163
feature: FragmentFeature26
feature: ModificationFeature4
cellular location: nucleoplasm
comment: Reactome DB_ID: 3009372
display name: SUPT5H

http://identifiers.org/pubmed/22762019
type: PublicationXref
id: 22762019
author: Kräusslich, Hans-Georg
author: Sundquist, WI
db: Pubmed
title: HIV-1 Assembly, Budding, and Maturation
source: Cold Spring Harb Perspect Med 2:a006924
year: 2012

VPR (P69726) protein
type: Protein
entity reference: UniProt:P69726 vpr
data source: Reactome
xref: UnificationXref213
xref: UnificationXref212
cellular location: nucleoplasm
comment: Reactome DB_ID: 175516

PC4 and SFRS1-interacting protein
type: Protein
entity reference: DFS70
data source: Reactome
name: PSIP1_HUMAN
xref: UnificationXref210
xref: UnificationXref211
feature: FragmentFeature29
cellular location: nucleoplasm
comment: Reactome DB_ID: 3008841
display name: PSIP1

Stoichiometry19
type: Stoichiometry
physical entity: p-S2,S5-POLR2A
stoichiometric coefficient: 1.0

Stoichiometry18
type: Stoichiometry
physical entity: DNA-directed RNA polymerase II 14.4 kDa polypeptide (EC 2.7.7.6) (RPB6) (RPB14.4)
stoichiometric coefficient: 1.0

Stoichiometry15
type: Stoichiometry
physical entity: FCP1P protein
stoichiometric coefficient: 1.0

Stoichiometry14
type: Stoichiometry
physical entity: Tat:P-TEFb(Cyclin T1:Cdk9) complex
stoichiometric coefficient: 1.0

Stoichiometry17
type: Stoichiometry
physical entity: RPABC4
stoichiometric coefficient: 1.0

Stoichiometry16
type: Stoichiometry
physical entity: POLR2H
stoichiometric coefficient: 1.0

Stoichiometry11
type: Stoichiometry
physical entity: C-2K
stoichiometric coefficient: 1.0

Stoichiometry10
type: Stoichiometry
physical entity: CycT1
stoichiometric coefficient: 1.0

Stoichiometry13
type: Stoichiometry
physical entity: Tat (P04608)
stoichiometric coefficient: 1.0

Stoichiometry12
type: Stoichiometry
physical entity: P-TEFb(Cyclin T1:Cdk9) complex
stoichiometric coefficient: 1.0

UniProt:P04578 env
type: ProteinReference
name: env
xref: UnificationXref19
comment: FUNCTION The surface protein gp120 (SU) attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. This interaction induces a structural rearrangement creating a high affinity binding site for a chemokine coreceptor like CXCR4 and/or CCR5. This peculiar 2 stage receptor-interaction strategy allows gp120 to maintain the highly conserved coreceptor-binding site in a cryptic conformation, protected from neutralizing antibodies. Since CD4 also displays a binding site for the disulfide-isomerase P4HB/PDI, a P4HB/PDI-CD4-CXCR4-gp120 complex may form. In that complex, P4HB/PDI could reach and reduce gp120 disulfide bonds, causing major conformational changes in gp120. TXN, another PDI family member could also be involved in disulfide rearrangements in Env during fusion. These changes are transmitted to the transmembrane protein gp41 and are thought to activate its fusogenic potential by unmasking its fusion peptide.FUNCTION The surface protein gp120 is a ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses. These interactions allow capture of viral particles at mucosal surfaces by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they take up antigen, process it, and present it to T-cells following migration to lymphoid organs. HIV subverts the migration properties of dendritic cells to gain access to CD4+ T-cells in lymph nodes. Virus transmission to permissive T-cells occurs either in trans (without DCs infection, through viral capture and transmission), or in cis (following DCs productive infection, through the usual CD4-gp120 interaction), thereby inducing a robust infection. In trans infection, bound virions remain infectious…
organism: Human immunodeficiency virus 1

Transcription initiation factor RAP30
type: Protein
entity reference: UniProt:P13984 GTF2F2
data source: Reactome
name: GTF2F2(2-249)
name: Transcription initiation factor IIF, beta subunit
xref: UnificationXref133
xref: UnificationXref132
feature: FragmentFeature19
cellular location: nucleoplasm
comment: Reactome DB_ID: 65567
display name: TFIIF-beta

Transcription initiation factor IIF, alpha subunit
type: Protein
entity reference: UniProt:P35269 GTF2F1
data source: Reactome
name: GTF2F1(2-517)
name: Transcription initiation factor RAP74
xref: UnificationXref135
xref: UnificationXref136
feature: FragmentFeature20
cellular location: nucleoplasm
comment: Reactome DB_ID: 65565
display name: TFIIF-alpha

HSPC130
type: Protein
entity reference: TH1
data source: Reactome
name: Negative elongation factor C/D
name: NELF-C/D
name: TH1-like protein
name: NELF-C/D protein
xref: UnificationXref142
xref: UnificationXref143
feature: FragmentFeature21
cellular location: nucleoplasm
comment: Reactome DB_ID: 112428
display name: NELFCD

DNA-directed RNA polymerase II 13.3 kDa polypeptide
type: Protein
entity reference: POLR2J
data source: Reactome
name: POLR2J
xref: UnificationXref118
xref: UnificationXref119
feature: FragmentFeature15
cellular location: nucleoplasm
comment: Reactome DB_ID: 63531
display name: RPB11

POLR2G
type: Protein
entity reference: UniProt:P62487 POLR2G
data source: Reactome
name: DNA-directed RNA polymerase II 19 kDa polypeptide
xref: UnificationXref122
xref: UnificationXref121
feature: FragmentFeature16
cellular location: nucleoplasm
comment: Reactome DB_ID: 63517
display name: RPB7

DNA-directed RNA polymerase II 16 kDa polypeptide
type: Protein
entity reference: UniProt:O15514 POLR2D
data source: Reactome
name: POLR2D(2-142)
xref: UnificationXref125
xref: UnificationXref124
feature: FragmentFeature17
cellular location: nucleoplasm
comment: Reactome DB_ID: 63508
display name: RPB4

DNA-directed RNA polymerase II 7.6 kDa polypeptide
type: Protein
entity reference: POLR2L
data source: Reactome
name: HsRPABC5
name: RPABC5
name: POLR2L
name: DNA-directed RNA polymerase II 7.6 kDa polypeptide (EC 2.7.7.6) (RPB10) (RPB7.6)
name: RPB7.6
xref: UnificationXref128
xref: UnificationXref127
feature: FragmentFeature18
cellular location: nucleoplasm
comment: Reactome DB_ID: 83715
display name: RPB10

RNA polymerase II subunit 3
type: Protein
entity reference: A-152E5.7
data source: Reactome
name: RPB31
name: RPB33
name: DNA-directed RNA polymerase II 33 kDa polypeptide
name: POLR2C
xref: UnificationXref109
xref: UnificationXref110
feature: FragmentFeature12
cellular location: nucleoplasm
comment: Reactome DB_ID: 63506
display name: RPB3

DNA-directed RNA polymerase II 140 kDa polypeptide
type: Protein
entity reference: UniProt:P30876 POLR2B
data source: Reactome
name: RNA polymerase II subunit 2
name: POLR2B
xref: UnificationXref113
xref: UnificationXref112
feature: FragmentFeature13
cellular location: nucleoplasm
comment: Reactome DB_ID: 63504
display name: RPB2

RPB14.5
type: Protein
entity reference: UniProt:P36954 POLR2I
data source: Reactome
name: POLR2I
name: DNA-directed RNA polymerase II 14.5 kDa polypeptide
xref: UnificationXref115
xref: UnificationXref116
feature: FragmentFeature14
cellular location: nucleoplasm
comment: Reactome DB_ID: 63525
display name: RPB9

Stoichiometry31
type: Stoichiometry
physical entity: TFIIF
stoichiometric coefficient: 1.0

Stoichiometry30
type: Stoichiometry
physical entity: Transcription initiation factor IIF, alpha subunit
stoichiometric coefficient: 1.0

Stoichiometry23
type: Stoichiometry
physical entity: RPB14.5
stoichiometric coefficient: 1.0

Stoichiometry24
type: Stoichiometry
physical entity: DNA-directed RNA polymerase II 13.3 kDa polypeptide
stoichiometric coefficient: 1.0

Stoichiometry21
type: Stoichiometry
physical entity: RNA polymerase II subunit 3
stoichiometric coefficient: 1.0

Stoichiometry22
type: Stoichiometry
physical entity: DNA-directed RNA polymerase II 140 kDa polypeptide
stoichiometric coefficient: 1.0

UniProt:O15514 POLR2D
type: ProteinReference
name: POLR2D
xref: UnificationXref123
comment: SUBUNIT Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex.SIMILARITY Belongs to the eukaryotic RPB4 RNA polymerase subunit family.
organism: Homo sapiens

Stoichiometry27
type: Stoichiometry
physical entity: DNA-directed RNA polymerase II 7.6 kDa polypeptide
stoichiometric coefficient: 1.0

Stoichiometry28
type: Stoichiometry
physical entity: RNA Polymerase II holoenzyme complex (hyperphosphorylated)
stoichiometric coefficient: 1.0

Stoichiometry25
type: Stoichiometry
physical entity: POLR2G
stoichiometric coefficient: 1.0

Stoichiometry26
type: Stoichiometry
physical entity: DNA-directed RNA polymerase II 16 kDa polypeptide
stoichiometric coefficient: 1.0

Stoichiometry29
type: Stoichiometry
physical entity: Transcription initiation factor RAP30
stoichiometric coefficient: 1.0

http://identifiers.org/pubmed/16482214
type: PublicationXref
id: 16482214
author: Mizuuchi, M
author: Craigie, R
author: Burke TR, Jr
author: Li, M
db: Pubmed
title: Retroviral DNA integration: reaction pathway and critical intermediates
source: EMBO J 25:1295-304
year: 2006

p-S2,S5-POLR2A
type: Protein
entity reference: POLR2
data source: Reactome
name: RNA Pol II (Ser2 phosphorylated) largest subunit
name: DNA-directed RNA polymerase II largest subunit
xref: UnificationXref103
xref: UnificationXref104
feature: FragmentFeature10
feature: ModificationFeature1
feature: ModificationFeature2
feature: EntityFeature1
cellular location: nucleoplasm
comment: Reactome DB_ID: 113420
display name: RPB1

DNA-directed RNA polymerase II 23 kDa polypeptide (EC 2.7.7.6) (RPB25) (XAP4) (RPB5)
type: Protein
entity reference: POLR2E
data source: Reactome
name: POLR2E
name: HsRPABC1
name: RPABC1
name: RPB5
name: RPB25
name: DNA-directed RNA polymerase II 23 kDa polypeptide
xref: UnificationXref106
xref: UnificationXref107
feature: FragmentFeature11
cellular location: nucleoplasm
comment: Reactome DB_ID: 83713
display name: XAP4

RPABC4
type: Protein
entity reference: POLR2K
data source: Reactome
name: RPB7.0
name: RPB10alpha
name: ABC10-alpha
name: DNA-directed RNA polymerases I, II, and III 7.0 kDa polypeptide
xref: UnificationXref96
xref: UnificationXref97
feature: FragmentFeature8
cellular location: nucleoplasm
comment: Reactome DB_ID: 63537
display name: POLR2K

http://identifiers.org/pubmed/8883604
type: PublicationXref
id: 8883604
author: Farnet, CM
author: Bushman, FD
db: Pubmed
title: HIV cDNA integration: molecular biology and inhibitor development
source: AIDS 10:S3-11
year: 1996

DNA-directed RNA polymerase II 14.4 kDa polypeptide (EC 2.7.7.6) (RPB6) (RPB14.4)
type: Protein
entity reference: POLR2F
data source: Reactome
name: RPB14.4
name: DNA-directed RNA polymerase II 14.4 kDa polypeptide
name: HsRPABC2
name: POLR2F(1-127)
name: RPABC2
xref: UnificationXref99
xref: UnificationXref100
feature: FragmentFeature9
cellular location: nucleoplasm
comment: Reactome DB_ID: 83714
display name: RPB6

FCP1P protein
type: Protein
entity reference: UniProt:Q9Y5B0 CTDP1
data source: Reactome
xref: UnificationXref90
xref: UnificationXref91
feature: FragmentFeature6
cellular location: nucleoplasm
comment: Reactome DB_ID: 113416
display name: CTDP1

POLR2H
type: Protein
entity reference: POLR2H
data source: Reactome
name: DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide
name: RPABC3
name: RPB17
xref: UnificationXref93
xref: UnificationXref94
feature: FragmentFeature7
cellular location: nucleoplasm
comment: Reactome DB_ID: 63523
display name: RPB8

C-2K
type: Protein
entity reference: UniProt:P50750 CDK9
data source: Reactome
name: Serine/threonine-protein kinase PITALRE
name: Cell division protein kinase 9
name: Cdk 9 protein
xref: UnificationXref80
xref: UnificationXref81
feature: FragmentFeature5
cellular location: nucleoplasm
comment: Reactome DB_ID: 51801
display name: CDK9

Tat (P04608)
type: Protein
entity reference: UniProt:P04608 tat
data source: Reactome
xref: UnificationXref86
xref: UnificationXref85
cellular location: nucleoplasm
comment: Reactome DB_ID: 175856

VPU (P05919)
type: Protein
entity reference: UniProt:P05919 vpu
data source: Reactome
xref: UnificationXref69
xref: UnificationXref68
cellular location: plasma membrane
comment: Reactome DB_ID: 3149423

CycT1
type: Protein
entity reference: CCNT1
data source: Reactome
name: Cyclin T
name: Cyclin T1
xref: UnificationXref78
xref: UnificationXref77
feature: FragmentFeature4
cellular location: nucleoplasm
comment: Reactome DB_ID: 51611
display name: CCNT1

Stoichiometry40
type: Stoichiometry
physical entity: Transcription elongation factor SPT5
stoichiometric coefficient: 1.0

Stoichiometry42
type: Stoichiometry
physical entity: SPT4H1 protein
stoichiometric coefficient: 1.0

Stoichiometry41
type: Stoichiometry
physical entity: DSIF complex
stoichiometric coefficient: 1.0

Stoichiometry32
type: Stoichiometry
physical entity: RNA Polymerase II holoenzyme complex (hyperphosphorylated):TFIIF complex
stoichiometric coefficient: 1.0

Stoichiometry33
type: Stoichiometry
physical entity: HSPC130
stoichiometric coefficient: 1.0

Stoichiometry34
type: Stoichiometry
physical entity: Negative elongation factor A
stoichiometric coefficient: 1.0

Stoichiometry35
type: Stoichiometry
physical entity: Negative elongation factor B
stoichiometric coefficient: 1.0

Stoichiometry36
type: Stoichiometry
physical entity: phospho-RD
stoichiometric coefficient: 1.0

Stoichiometry37
type: Stoichiometry
physical entity: phospho-NELF complex
stoichiometric coefficient: 1.0

Stoichiometry38
type: Stoichiometry
physical entity: HIV-1 template:capped HIV-1 transcript hybrid
stoichiometric coefficient: 1.0

Stoichiometry39
type: Stoichiometry
physical entity: SPT4H1 protein
stoichiometric coefficient: 1.0

http://identifiers.org/pubmed/11527981
type: PublicationXref
id: 11527981
author: Gordon, JI
author: Farazi, TA
author: Waksman, G
db: Pubmed
title: The biology and enzymology of protein N-myristoylation
source: J Biol Chem 276:39501-4
year: 2001

http://identifiers.org/pubmed/15677323
type: PublicationXref
id: 15677323
author: Engelman, A
db: Pubmed
title: The ups and downs of gene expression and retroviral DNA integration
source: Proc Natl Acad Sci U S A 102:1275-6
year: 2005

Stoichiometry53
type: Stoichiometry
physical entity: Rev-multimer
stoichiometric coefficient: 1.0

Stoichiometry52
type: Stoichiometry
physical entity: Rev-bound HIV-1 mRNA
stoichiometric coefficient: 1.0

Stoichiometry51
type: Stoichiometry
physical entity: REV (P04618) protein
stoichiometric coefficient: 1.0

Stoichiometry50
type: Stoichiometry
physical entity: HIV-1 mRNA
stoichiometric coefficient: 1.0

gag
type: ProteinReference
name: UniProt:P04591 gag
xref: UnificationXref220
comment: FUNCTION Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu.FUNCTION Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.FUNCTION Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers.PTM Capsid protein p24 is phosphorylated.MISCELLANEOUS HIV-1 lineages are divided in three main groups, M (for Major), O (for Outlier), and N (for New, or Non-M, Non-O). The vast majority of strains found worldwide belong to the group M. Group O seems to be endemic to and largely confined to Cameroon and neighboring countries in West Central Africa, where these viruses represent a small minority of HIV-1 strains. The group N is represented by a limited number of isolates from Cameroonian persons. The group M is further subdivided in 9 clades or subtypes (A to D, F to H, J and K).SIMILARITY Belongs to the primate lentivirus group gag polyprotein family.SIMILARITY Contains 2 CCHC-type zinc fingers.
organism: Human immunodeficiency virus 1

Stoichiometry49
type: Stoichiometry
physical entity: HIV-1 template:capped HIV-1 transcript hybrid
stoichiometric coefficient: 1.0

Stoichiometry47
type: Stoichiometry
physical entity: RNA Polymerase II holoenzyme complex (hyperphosphorylated):TFIIF complex
stoichiometric coefficient: 1.0

Stoichiometry48
type: Stoichiometry
physical entity: phospho-NELF complex
stoichiometric coefficient: 1.0

Stoichiometry45
type: Stoichiometry
physical entity: Tat:P-TEFb(Cyclin T1:Cdk9) complex
stoichiometric coefficient: 1.0

cytosol
type: CellularLocationVocabulary
xref: UnificationXref1

Stoichiometry46
type: Stoichiometry
physical entity: FCP1P protein
stoichiometric coefficient: 1.0

Stoichiometry43
type: Stoichiometry
physical entity: Transcription elongation factor SPT5
stoichiometric coefficient: 1.0

Stoichiometry44
type: Stoichiometry
physical entity: phospho-DSIF complex
stoichiometric coefficient: 1.0

http://identifiers.org/pubmed/15613341
type: PublicationXref
id: 15613341
author: Skowronski, J
author: Hill, BT
db: Pubmed
title: Human N-myristoyltransferases form stable complexes with lentiviral nef and other viral and cellular substrate proteins
source: J Virol 79:1133-41
year: 2005

Stoichiometry62
type: Stoichiometry
physical entity: HMGA1_HUMAN
stoichiometric coefficient: 1.0

Stoichiometry61
type: Stoichiometry
physical entity: VPR (P69726) protein
stoichiometric coefficient: 1.0

gag-pol
type: ProteinReference
name: UniProt:P04585 gag-pol
xref: UnificationXref198
comment: FUNCTION Gag-Pol polyprotein and Gag polyprotein may regulate their own translation, by the binding genomic RNA in the 5'-UTR. At low concentration, Gag-Pol and Gag would promote translation, whereas at high concentration, the polyproteins encapsidate genomic RNA and then shutt off translation.FUNCTION Matrix protein p17 has two main functions: in infected cell, it targets Gag and Gag-pol polyproteins to the plasma membrane via a multipartite membrane-binding signal, that includes its myristoylated N-terminus. The second function is to play a role in nuclear localization of the viral genome at the very start of cell infection. Matrix protein is the part of the pre-integration complex. It binds in the cytoplasm the human BAF protein which prevent autointegration of the viral genome, and might be included in virions at the ration of zero to 3 BAF dimer per virion. The myristoylation signal and the NLS thus exert conflicting influences its subcellular localization. The key regulation of these motifs might be phosphorylation of a portion of MA molecules on the C-terminal tyrosine at the time of virus maturation, by virion-associated cellular tyrosine kinase. Implicated in the release from host cell mediated by Vpu.FUNCTION Capsid protein p24 forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. Most core are conical, with only 7% tubular. The core is constituted by capsid protein hexamer subunits. The core is disassembled soon after virion entry. Interaction with human PPIA/CYPA protects the virus from restriction by human TRIM5-alpha and from an unknown antiviral activity in human cells. This capsid restriction by TRIM5 is one of the factors which restricts HIV-1 to the human species.FUNCTION Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers. Facilitates rearangement of nucleic acid secondary structure during retrotranscription of genomic RNA. This ca…
organism: Human immunodeficiency virus 1

Stoichiometry64
type: Stoichiometry
physical entity: Matrix
stoichiometric coefficient: 1.0

Stoichiometry63
type: Stoichiometry
physical entity: VIF (P69723) protein
stoichiometric coefficient: 1.0

Stoichiometry60
type: Stoichiometry
physical entity: PC4 and SFRS1-interacting protein
stoichiometric coefficient: 1.0

Stoichiometry58
type: Stoichiometry
physical entity: viral DNA with 3' sticky ends
stoichiometric coefficient: 1.0

Stoichiometry59
type: Stoichiometry
physical entity: IN bound to sticky 3' ends of viral DNA
stoichiometric coefficient: 1.0

Stoichiometry54
type: Stoichiometry
physical entity: BAF protein
stoichiometric coefficient: 1.0

Stoichiometry55
type: Stoichiometry
physical entity: IN (Integrase) (P04585) protein
stoichiometric coefficient: 2.0

Stoichiometry56
type: Stoichiometry
physical entity: viral minus strand DNA with sticky 3' end
stoichiometric coefficient: 1.0

Stoichiometry57
type: Stoichiometry
physical entity: viral plus strand DNA with sticky 3' end
stoichiometric coefficient: 1.0

KIAA1182
type: ProteinReference
name: UniProt:Q8WX92 NELFB
name: NELFB
name: COBRA1
xref: UnificationXref147
comment: FUNCTION Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding.SUBUNIT The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE. Interacts with the first BRCT repeat of BRCA1. Interacts with KIAA1191.TISSUE SPECIFICITY Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.SIMILARITY Belongs to the NELF-B family.
organism: Homo sapiens

http://identifiers.org/pubmed/9356449
type: PublicationXref
id: 9356449
author: Lewis, DE
author: Herrmann, CH
author: Aguilar-Cordova, E
author: Tang, DN
author: Yang, X
author: Rice, AP
author: Gold, MO
db: Pubmed
title: TAK, an HIV Tat-associated kinase, is a member of the cyclin-dependent family of protein kinases and is induced by activation of peripheral blood lymphocytes and differentiation of promonocytic cell lines
source: Proc Natl Acad Sci U S A 94:12331-6
year: 1997

http://identifiers.org/pubmed/15218037
type: PublicationXref
id: 15218037
author: Krausslich, HG
author: Stray, KM
author: Sundquist, WI
author: Morham, SG
author: Ghaffarian, S
author: Garrus, JE
author: Muller, B
author: McKinnon, R
author: Stuchell, MD
db: Pubmed
title: The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding
source: J Biol Chem 279:36059-71
year: 2004

Stoichiometry66
type: Stoichiometry
physical entity: REV (P04618) protein
stoichiometric coefficient: 1.0

Stoichiometry65
type: Stoichiometry
physical entity: VPU (P05919) protein
stoichiometric coefficient: 1.0

Stoichiometry68
type: Stoichiometry
physical entity: BAF protein
stoichiometric coefficient: 1.0

Stoichiometry67
type: Stoichiometry
physical entity: p6 protein
stoichiometric coefficient: 1.0

Stoichiometry69
type: Stoichiometry
physical entity: PC4 and SFRS1-interacting protein
stoichiometric coefficient: 1.0

Stoichiometry70
type: Stoichiometry
physical entity: VPR (P69726) protein
stoichiometric coefficient: 1.0

Stoichiometry71
type: Stoichiometry
physical entity: HMGA1_HUMAN
stoichiometric coefficient: 1.0

Stoichiometry72
type: Stoichiometry
physical entity: VIF (P69723) protein
stoichiometric coefficient: 1.0

Stoichiometry73
type: Stoichiometry
physical entity: Matrix
stoichiometric coefficient: 1.0

Stoichiometry74
type: Stoichiometry
physical entity: VPU (P05919) protein
stoichiometric coefficient: 1.0

Stoichiometry75
type: Stoichiometry
physical entity: REV (P04618) protein
stoichiometric coefficient: 1.0

Glycosylated Envelope glycoprotein gp160
type: Protein
entity reference: UniProt:P04578 env
data source: Reactome
xref: UnificationXref32
xref: UnificationXref31
cellular location: Golgi membrane
comment: Reactome DB_ID: 3149443

Envelope glycoprotein gp160
type: Protein
entity reference: UniProt:P04578 env
data source: Reactome
xref: UnificationXref20
xref: UnificationXref21
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 189343

Transmembrane protein gp41 (P04578)
type: Protein
entity reference: UniProt:P04578 env
data source: Reactome
xref: UnificationXref41
xref: UnificationXref42
feature: FragmentFeature2
cellular location: cytosol
comment: Reactome DB_ID: 189359

Surface protein gp120 (P04578)
type: Protein
entity reference: UniProt:P04578 env
data source: Reactome
xref: UnificationXref38
xref: UnificationXref37
feature: FragmentFeature1
cellular location: cytosol
comment: Reactome DB_ID: 189269

VPR (P69726) protein
type: Protein
entity reference: UniProt:P69726 vpr
data source: Reactome
xref: UnificationXref8
xref: UnificationXref7
cellular location: mitochondrial intermembrane space
comment: Reactome DB_ID: 180888

VPR (P69726) protein
type: Protein
entity reference: UniProt:P69726 vpr
data source: Reactome
xref: UnificationXref4
xref: UnificationXref5
cellular location: cytosol
comment: Reactome DB_ID: 173193

Stoichiometry79
type: Stoichiometry
physical entity: IN bound to sticky 3' ends of viral DNA
stoichiometric coefficient: 1.0

Stoichiometry78
type: Stoichiometry
physical entity: genomic DNA with staggered 5' ends
stoichiometric coefficient: 1.0

Stoichiometry77
type: Stoichiometry
physical entity: IN bound to sticky 3' ends of viral DNA
stoichiometric coefficient: 1.0

Stoichiometry76
type: Stoichiometry
physical entity: p6 protein
stoichiometric coefficient: 1.0

Stoichiometry81
type: Stoichiometry
physical entity: IN:viral DNA bound to host genomic DNA
stoichiometric coefficient: 1.0

Stoichiometry82
type: Stoichiometry
physical entity: genomic DNA with staggered 5' ends
stoichiometric coefficient: 1.0

Stoichiometry80
type: Stoichiometry
physical entity: Host genomic DNA
stoichiometric coefficient: 1.0

Stoichiometry85
type: Stoichiometry
physical entity: viral minus strand DNA (ful-length)
stoichiometric coefficient: 1.0

Stoichiometry86
type: Stoichiometry
physical entity: linear duplex viral DNA
stoichiometric coefficient: 1.0

Stoichiometry83
type: Stoichiometry
physical entity: IN (Integrase) (P04585) protein
stoichiometric coefficient: 2.0

Stoichiometry84
type: Stoichiometry
physical entity: viral plus strand DNA (full-length)
stoichiometric coefficient: 1.0

Furin
type: Protein
entity reference: PACE
data source: Reactome
xref: UnificationXref49
xref: UnificationXref50
feature: FragmentFeature3
cellular location: Golgi membrane
comment: Reactome DB_ID: 157049
display name: FURIN

VPU (P05919)
type: Protein
entity reference: UniProt:P05919 vpu
data source: Reactome
xref: UnificationXref60
xref: UnificationXref61
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 180550

VPU (P05919)
type: Protein
entity reference: UniProt:P05919 vpu
data source: Reactome
xref: UnificationXref64
xref: UnificationXref65
cellular location: Golgi membrane
comment: Reactome DB_ID: 3149439

http://identifiers.org/pubmed/12202041
type: PublicationXref
id: 12202041
author: Chen, H
author: Berry, C
author: Ecker, JR
author: Bushman, FD
author: Shinn, P
author: Schroder, AR
db: Pubmed
title: HIV-1 integration in the human genome favors active genes and local hotspots
source: Cell 110:521-9
year: 2002

Stoichiometry88
type: Stoichiometry
physical entity: N-myristoyl GAG (P04591) protein
stoichiometric coefficient: 1.0

Integration of viral DNA into host genomic DNA
type: Pathway
data source: Reactome
pathway component: Gap repair completes provirus integration
pathway component: Transesterification to connect viral DNA 3' ends to host DNA 5' ends
pathway component: Target DNA binding
xref: http://identifiers.org/pubmed/16482214
xref: UnificationXref263
xref: http://identifiers.org/pubmed/16291214
xref: UnificationXref264
xref: http://identifiers.org/pubmed/9188609
xref: http://identifiers.org/pubmed/8041787
xref: RelationshipXref8
xref: http://identifiers.org/pubmed/16175173
xref: PublicationXref18
pathway order: PathwayStep11
pathway order: PathwayStep13
pathway order: PathwayStep12
comment: Edited: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22
comment: Reviewed: Bushman, FD, 2006-10-30 22:19:13
comment: Authored: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22
comment: Following nuclear entry, the viral preintegration complex (PIC) must select a site for integration in a host cell chromosome, and then carry out the chemical steps of the reaction. At the chromosomal level, HIV has been found to favor active transcription units for integration. Subsequent studies established that the cellular PSIP1/LEDGF/p75 protein is important in this reaction. PSIP1/LEDGF/p75 binds tightly to HIV integrase, and also to chromatin. Knocking down PSIP1/LEDGF/p75 in cells resulted in several perturbations of integration targeting in vivo, including reduced integration in transcription units. Thus PSIP1/LEDGF/p75 has been hypothesized to act as a tethering factor that dictates at least in part the placement of HIV integration sites. The integration target DNA is also expected to be coated with nucleosomes. Tests of integration into mononucleosomes in vitro have shown that wrapping integration target DNA actually boosts integration activity. Kinked positions on the DNA gyre are particularly favored for integration. Integration does not take place at a unique sequence in the integration target DNA (i.e. it is not like a restriction enzyme). However, favored and disfavored primary sequences can be detected when many integration sites are aligned. Synthesis and testing of favored HIV integration sites showed that they were favored for integration by PICs in vitro. After a target DNA is bound, the integration reactions take place via a single-step transesterification. Integration of both ends of the viral DNA, followed by melting of the target DNA segments between the points of joining, yields single stranded gaps at each host-virus DNA junction, and a two base overhang derived from the viral DNA. The manner by which this intermediate is subsequently repaired to yield the fully integrated provirus is unclear. For many parasitic DNA replication reactions, the parasite carries out reaction steps only up to a point that the host cannot easily reverse, forcing…
organism: Human immunodeficiency virus 1

Stoichiometry87
type: Stoichiometry
physical entity: Host genomic DNA
stoichiometric coefficient: 1.0

Stoichiometry89
type: Stoichiometry
physical entity: ubiquitin
stoichiometric coefficient: 1.0

Stoichiometry94
type: Stoichiometry
physical entity: VPS37A
stoichiometric coefficient: 1.0

Stoichiometry95
type: Stoichiometry
physical entity: VPS28
stoichiometric coefficient: 1.0

Stoichiometry96
type: Stoichiometry
physical entity: VPS37B
stoichiometric coefficient: 1.0

Stoichiometry97
type: Stoichiometry
physical entity: VPS37C
stoichiometric coefficient: 1.0

Stoichiometry90
type: Stoichiometry
physical entity: N-myristoyl GAG (P04591) protein
stoichiometric coefficient: 1.0

Stoichiometry91
type: Stoichiometry
physical entity: ubiquitin
stoichiometric coefficient: 1.0

http://identifiers.org/pubmed/1310932
type: PublicationXref
id: 1310932
author: Sil, A
author: Pryciak, PM
author: Varmus, HE
db: Pubmed
title: Retroviral integration into minichromosomes in vitro
source: EMBO J 11:291-303
year: 1992

Stoichiometry92
type: Stoichiometry
physical entity: Tumor susceptibility gene 101 protein
stoichiometric coefficient: 1.0

Stoichiometry93
type: Stoichiometry
physical entity: VPS37D
stoichiometric coefficient: 1.0

nucleoplasm
type: CellularLocationVocabulary
xref: UnificationXref75

http://identifiers.org/pubmed/21289126
type: PublicationXref
id: 21289126
author: Ono, Akira
author: Chukkapalli, Vineela
author: Derse, David
author: Inlora, Jingga
db: Pubmed
title: Gag localization and virus-like particle release mediated by the matrix domain of human T-lymphotropic virus type 1 Gag are less dependent on phosphatidylinositol-(4,5)-bisphosphate than those mediated by the matrix domain of HIV-1 Gag
source: J. Virol. 85:3802-10
year: 2011

Stoichiometry99
type: Stoichiometry
physical entity: N-myristoyl GAG
stoichiometric coefficient: 1.0

NMT2
type: ProteinReference
name: UniProt:O60551 NMT2
xref: UnificationXref282
comment: FUNCTION Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.CATALYTIC ACTIVITY Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.SIMILARITY Belongs to the NMT family.CAUTION Sequence AAF36406.2 was incorrectly indicated as originating from bovine.
organism: Homo sapiens

Stoichiometry98
type: Stoichiometry
physical entity: ubiquitin
stoichiometric coefficient: 1.0

http://identifiers.org/pubmed/2015625
type: PublicationXref
id: 2015625
author: Malim, MH
author: Cullen, BR
db: Pubmed
title: HIV-1 structural gene expression requires the binding of multiple Rev monomers to the viral RRE: implications for HIV-1 latency
source: Cell 65:241-8
year: 1991

UnificationXref289
type: UnificationXref
id: REACT_24666
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24666.2

UnificationXref288
type: UnificationXref
id: 939205
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939205

UniProt:Q9H9H4 VPS37B
type: ProteinReference
name: VPS37B
xref: UnificationXref372
comment: FUNCTION Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.SUBUNIT Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoechiometry. Interacts with TSG101, VPS28, MVB12A and MVB12B. Interacts with CEP55. Interacts with IST1.TISSUE SPECIFICITY Widely expressed. Expressed in macrophages and lymphocytes.SIMILARITY Belongs to the VPS37 family.SIMILARITY Contains 1 VPS37 C-terminal domain.
organism: Homo sapiens

UnificationXref287
type: UnificationXref
id: P62979
db: UniProt

UnificationXref286
type: UnificationXref
id: REACT_115774
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_115774.2

UnificationXref285
type: UnificationXref
id: 184392
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184392

UnificationXref284
type: UnificationXref
id: REACT_117497
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_117497.1

UnificationXref283
type: UnificationXref
id: 184377
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184377

UnificationXref282
type: UnificationXref
id: O60551
db: UniProt

UnificationXref281
type: UnificationXref
id: REACT_117898
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_117898.2

UnificationXref280
type: UnificationXref
id: 184427
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184427

http://identifiers.org/pubmed/8163529
type: PublicationXref
id: 8163529
author: Ruysschaert, J M
author: Basak, A
author: Marshall, G
author: Vandenbranden, M
author: Howard, S C
author: Jean, F
author: Jacob, G S
author: Kompelli, A
author: Cogniaux, J
author: Decroly, E
db: Pubmed
title: The convertases furin and PC1 can both cleave the human immunodeficiency virus (HIV)-1 envelope glycoprotein gp160 into gp120 (HIV-1 SU) and gp41 (HIV-I TM)
source: J. Biol. Chem. 269:12240-7
year: 1994

http://identifiers.org/pubmed/22553496
type: PublicationXref
id: 22553496
author: Malim, Michael H
author: Bieniasz, Paul D
db: Pubmed
title: HIV Restriction Factors and Mechanisms of Evasion
source: Cold Spring Harb Perspect Med 2:a006940
year: 2012

HIV-1 mRNA
type: Rna
entity reference: EMBL:AF033819
data source: Reactome
xref: UnificationXref183
xref: UnificationXref182
cellular location: nucleoplasm
comment: Reactome DB_ID: 165533

Spliced Env mRNA
type: Rna
entity reference: EMBL:AF033819
data source: Reactome
xref: UnificationXref14
xref: UnificationXref15
cellular location: cytosol
comment: Reactome DB_ID: 187215

HIV-1 unspliced RNA
type: Rna
entity reference: EMBL:AF033819
data source: Reactome
xref: UnificationXref12
xref: UnificationXref13
cellular location: cytosol
comment: Reactome DB_ID: 187209

UnificationXref279
type: UnificationXref
id: MOD:00068
db: MOD

UnificationXref276
type: UnificationXref
id: CHEBI:15346
db: ChEBI

UnificationXref275
type: UnificationXref
id: REACT_13331
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_13331.2

UnificationXref278
type: UnificationXref
id: REACT_2732
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_2732.2

UnificationXref277
type: UnificationXref
id: 76194
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=76194

UnificationXref272
type: UnificationXref
id: REACT_115916
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_115916.3

UnificationXref271
type: UnificationXref
id: 187213
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=187213

UnificationXref274
type: UnificationXref
id: 184174
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184174

UnificationXref273
type: UnificationXref
id: CHEBI:15532
db: ChEBI

UnificationXref270
type: UnificationXref
id: REACT_9790
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9790.3

UniProt:P62987 UBA52
type: ProteinReference
name: UBCEP2
name: UBA52
xref: UnificationXref290
comment: FUNCTION Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.FUNCTION Ribosomal protein L40 is a component of the 60S subunit of the ribosome.MISCELLANEOUS Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.MISCELLANEOUS For a better understanding, features related to ubiquitin are only indicated for the first chain.SIMILARITY In the N-terminal section; belongs to the ubiquitin family.SIMILARITY In the …
organism: Homo sapiens

endosome membrane
type: CellularLocationVocabulary
xref: UnificationXref325

http://identifiers.org/pubmed/20082634
type: PublicationXref
id: 20082634
author: Tazi, Jamal
author: Ayadi, Lilia
author: Marchand, Virginie
author: Bakkour, Nadia
author: Aboufirassi, Amina
author: Branlant, Christiane
db: Pubmed
title: Alternative splicing: regulation of HIV-1 multiplication as a target for therapeutic action
source: FEBS J. 277:867-76
year: 2010

UnificationXref294
type: UnificationXref
id: 939214
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939214

UBA80
type: ProteinReference
name: UniProt:P62979 RPS27A
name: RPS27A
name: UBCEP1
xref: UnificationXref287
comment: FUNCTION Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.FUNCTION Ribosomal protein S27a is a component of the 40S subunit of the ribosome.MISCELLANEOUS Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.MISCELLANEOUS For a better understanding, features related to ubiquitin are only indicated for the first chain.SIMILARITY In the N-terminal section; belongs to the ubiquitin family.SIMILARITY In the…
organism: Homo sapiens

UnificationXref293
type: UnificationXref
id: P0CG47
db: UniProt

UnificationXref296
type: UnificationXref
id: 939213
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939213

UnificationXref295
type: UnificationXref
id: REACT_24747
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24747.2

UnificationXref298
type: UnificationXref
id: 939230
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939230

UnificationXref297
type: UnificationXref
id: REACT_24504
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24504.2

CoA-SH
type: SmallMoleculeReference
name: Coenzyme A
name: CoA
name: CoA [ChEBI:15346]
xref: UnificationXref276

UnificationXref299
type: UnificationXref
id: REACT_24537
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24537.2

UnificationXref290
type: UnificationXref
id: P62987
db: UniProt

UnificationXref292
type: UnificationXref
id: REACT_24649
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24649.2

UnificationXref291
type: UnificationXref
id: 939203
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939203

UnificationXref230
type: UnificationXref
id: REACT_7519
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7519.3

UnificationXref233
type: UnificationXref
id: 175430
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175430

UnificationXref234
type: UnificationXref
id: REACT_7114
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7114.2

UnificationXref231
type: UnificationXref
id: 175300
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175300

UnificationXref232
type: UnificationXref
id: REACT_7718
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7718.3

UnificationXref238
type: UnificationXref
id: REACT_7748
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7748.1

UnificationXref237
type: UnificationXref
id: 175158
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175158

UnificationXref236
type: UnificationXref
id: REACT_7635
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7635.2

WBSCR24
type: ProteinReference
name: UniProt:Q86XT2 VPS37D
name: VPS37D
xref: UnificationXref363
comment: FUNCTION Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.SUBUNIT Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoechiometry. Interacts with TSG101 and MVB12A.DISEASE Note=VPS37D is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.SIMILARITY Belongs to the VPS37 family.SIMILARITY Contains 1 VPS37 C-terminal domain.
organism: Homo sapiens

UnificationXref235
type: UnificationXref
id: 175416
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175416

UnificationXref239
type: UnificationXref
id: 177527
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=177527

UnificationXref240
type: UnificationXref
id: REACT_9170
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9170.1

UnificationXref241
type: UnificationXref
id: 175520
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175520

UnificationXref242
type: UnificationXref
id: REACT_9149
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9149.1

UnificationXref243
type: UnificationXref
id: 175224
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175224

UnificationXref244
type: UnificationXref
id: REACT_9176
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9176.1

UnificationXref245
type: UnificationXref
id: 175108
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175108

UnificationXref247
type: UnificationXref
id: 175339
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175339

UnificationXref246
type: UnificationXref
id: REACT_9054
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9054.2

UnificationXref249
type: UnificationXref
id: 175148
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175148

UnificationXref248
type: UnificationXref
id: REACT_9250
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9250.1

Adenosine 5'-triphosphate
type: SmallMolecule
entity reference: Adenosine 5'-triphosphate
data source: Reactome
xref: UnificationXref169
xref: UnificationXref170
cellular location: nucleoplasm
comment: Reactome DB_ID: 29358
display name: ATP

Adenosine 5'-diphosphate
type: SmallMolecule
entity reference: ADP [ChEBI:16761]
data source: Reactome
xref: UnificationXref172
xref: UnificationXref173
cellular location: nucleoplasm
comment: Reactome DB_ID: 113582
display name: ADP

myristoyl-CoA
type: SmallMolecule
entity reference: Myristoyl-CoA
data source: Reactome
xref: UnificationXref275
xref: UnificationXref274
cellular location: cytosol
comment: Reactome DB_ID: 184174
display name: MYS-CoA

coenzyme A
type: SmallMolecule
entity reference: CoA-SH
data source: Reactome
xref: UnificationXref278
xref: UnificationXref277
cellular location: cytosol
comment: Reactome DB_ID: 76194
display name: CoA-SH

UnificationXref255
type: UnificationXref
id: 175411
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175411

UnificationXref256
type: UnificationXref
id: REACT_7761
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7761.3

Adenosine 5'-triphosphate
type: SmallMoleculeReference
name: ATP
name: ATP [ChEBI:15422]
xref: UnificationXref168

UnificationXref253
type: UnificationXref
id: 175036
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175036

UnificationXref254
type: UnificationXref
id: REACT_7783
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7783.3

UnificationXref251
type: UnificationXref
id: 164523
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=164523

UnificationXref252
type: UnificationXref
id: REACT_9048
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9048.2

UnificationXref250
type: UnificationXref
id: REACT_9115
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9115.1

UnificationXref259
type: UnificationXref
id: 175486
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175486

UnificationXref258
type: UnificationXref
id: REACT_7750
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7750.1

UnificationXref257
type: UnificationXref
id: 175124
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175124

RelationshipXref1
type: RelationshipXref
id: GO:0004252
db: GENE ONTOLOGY
relationship type: gene ontology term for cellular function

RelationshipXref2
type: RelationshipXref
id: 156993
db: Reactome Database ID Release 45
relationship type: Same Catalyst Activity
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=156993

RelationshipXref3
type: RelationshipXref
id: GO:0019082
db: GENE ONTOLOGY
relationship type: gene ontology term for cellular process

RelationshipXref4
type: RelationshipXref
id: GO:0016301
db: GENE ONTOLOGY
relationship type: gene ontology term for cellular function

RelationshipXref5
type: RelationshipXref
id: 170699
db: Reactome Database ID Release 45
relationship type: Same Catalyst Activity
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=170699

RelationshipXref6
type: RelationshipXref
id: GO:0008907
db: GENE ONTOLOGY
relationship type: gene ontology term for cellular function

RelationshipXref7
type: RelationshipXref
id: 175070
db: Reactome Database ID Release 45
relationship type: Same Catalyst Activity
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175070

UnificationXref264
type: UnificationXref
id: REACT_8990
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_8990.2

RelationshipXref8
type: RelationshipXref
id: GO:0075713
db: GENE ONTOLOGY
relationship type: gene ontology term for cellular process

UnificationXref265
type: UnificationXref
id: 165027
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=165027

UnificationXref266
type: UnificationXref
id: REACT_6161
db: Reactome
id version: 5
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6161.5

RelationshipXref9
type: RelationshipXref
id: GO:0006369
db: GENE ONTOLOGY
relationship type: gene ontology term for cellular process

UnificationXref267
type: UnificationXref
id: 167168
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=167168

UnificationXref260
type: UnificationXref
id: REACT_7455
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7455.1

UnificationXref261
type: UnificationXref
id: 164506
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=164506

UnificationXref262
type: UnificationXref
id: REACT_9001
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9001.2

UnificationXref263
type: UnificationXref
id: 175567
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175567

http://identifiers.org/pubmed/12407101
type: PublicationXref
id: 12407101
author: De Clercq, E
author: Debyser, Z
author: Van Beeumen, J
author: Cherepanov, P
author: Proost, P
author: Maertens, G
author: Engelborghs, Y
author: Devreese, B
db: Pubmed
title: HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells
source: J Biol Chem 278:372-81
year: 2003

POLR2F
type: ProteinReference
name: UniProt:P61218 POLR2F
name: POLRF
xref: UnificationXref98
comment: SIMILARITY Belongs to the archaeal RpoK/eukaryotic RPB6 RNA polymerase subunit family.
organism: Homo sapiens

UnificationXref269
type: UnificationXref
id: 184393
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184393

UnificationXref268
type: UnificationXref
id: REACT_6241
db: Reactome
id version: 4
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6241.4

http://identifiers.org/pubmed/9525617
type: PublicationXref
id: 9525617
author: Coren, LV
author: Henderson, LE
author: Oroszlan, S
author: Ott, DE
author: Arthur, LO
author: Sowder RC, 2nd
author: Yoshinaka, Y
author: Kane, BP
author: Johnson, DG
author: Copeland, TD
db: Pubmed
title: Ubiquitin is covalently attached to the p6Gag proteins of human immunodeficiency virus type 1 and simian immunodeficiency virus and to the p12Gag protein of Moloney murine leukemia virus
source: J Virol 72:2962-8
year: 1998

http://identifiers.org/pubmed/1548743
type: PublicationXref
id: 1548743
author: Serabyn, SA
author: Bess Jr, JW
author: Fenselau, C
author: Henderson, LE
author: Arthur, LO
author: Bryant, DK
author: Johnson, DG
author: Bowers, MA
author: Sowder II, RC
db: Pubmed
title: Gag proteins of the highly replicative MN strain of human immunodeficiency virus type 1: posttranslational modifications, proteolytic processings, and complete amino acid sequences
source: J Virol 66:1856-65
year: 1992

http://identifiers.org/pubmed/9491887
type: PublicationXref
id: 9491887
author: Fang, SM
author: Wei, P
author: Jones, KA
author: Garber, ME
author: Fischer, WH
db: Pubmed
title: A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA
source: Cell 92:451-62
year: 1998

ubiquitin (UBC 7)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref404
xref: UnificationXref405
feature: FragmentFeature81
cellular location: plasma membrane
comment: Reactome DB_ID: 3149468
display name: UBC(457-532)

ubiquitin (UBC 6)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref403
xref: UnificationXref402
feature: FragmentFeature80
cellular location: plasma membrane
comment: Reactome DB_ID: 3149459
display name: UBC(381-456)

ubiquitin (UBC 9)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref409
xref: UnificationXref408
feature: FragmentFeature83
cellular location: plasma membrane
comment: Reactome DB_ID: 3149466
display name: UBC(609-684)

ubiquitin (UBC 8)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref407
xref: UnificationXref406
feature: FragmentFeature82
cellular location: plasma membrane
comment: Reactome DB_ID: 3149462
display name: UBC(533-608)

N-myristoyl GAG
type: Protein
entity reference: gag
data source: Reactome
xref: UnificationXref412
xref: UnificationXref413
feature: ModificationFeature5
cellular location: plasma membrane
comment: Reactome DB_ID: 184380

http://identifiers.org/pubmed/8041787
type: PublicationXref
id: 8041787
author: Ellison, V
author: Brown, PO
db: Pubmed
title: A stable complex between integrase and viral DNA ends mediates human immunodeficiency virus integration in vitro
source: Proc Natl Acad Sci U S A 91:7316-20
year: 1994

UnificationXref201
type: UnificationXref
id: 175079
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175079

UnificationXref200
type: UnificationXref
id: REACT_7052
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7052.2

http://identifiers.org/pubmed/1329090
type: PublicationXref
id: 1329090
author: Pryciak, PM
author: Muller, HP
author: Varmus, HE
db: Pubmed
title: Simian virus 40 minichromosomes as targets for retroviral integration in vivo
source: Proc Natl Acad Sci U S A 89:9237-41
year: 1992

UnificationXref202
type: UnificationXref
id: REACT_7325
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7325.2

UnificationXref203
type: UnificationXref
id: 175429
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175429

UnificationXref204
type: UnificationXref
id: REACT_7330
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7330.2

http://identifiers.org/pubmed/9188609
type: PublicationXref
id: 9188609
author: Miller, MD
author: Bushman, FD
db: Pubmed
title: Human immunodeficiency virus type 1 preintegration complexes: studies of organization and composition
source: J Virol 71:5382-90
year: 1997

UnificationXref205
type: UnificationXref
id: 175146
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175146

UnificationXref206
type: UnificationXref
id: REACT_7525
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7525.1

UnificationXref207
type: UnificationXref
id: 175332
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175332

UnificationXref208
type: UnificationXref
id: REACT_7266
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7266.1

UnificationXref209
type: UnificationXref
id: O75475
db: UniProt

UnificationXref212
type: UnificationXref
id: 175516
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175516

UnificationXref211
type: UnificationXref
id: REACT_161460
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_161460.2

UnificationXref210
type: UnificationXref
id: 3008841
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3008841

UnificationXref84
type: UnificationXref
id: P04608
db: UniProt

UnificationXref85
type: UnificationXref
id: 175856
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175856

UnificationXref86
type: UnificationXref
id: REACT_7042
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7042.3

UnificationXref87
type: UnificationXref
id: 167237
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=167237

UnificationXref88
type: UnificationXref
id: REACT_6496
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6496.2

UnificationXref89
type: UnificationXref
id: Q9Y5B0
db: UniProt

UniProt:P35269 GTF2F1
type: ProteinReference
name: RAP74
name: GTF2F1
xref: UnificationXref134
comment: FUNCTION TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.SUBUNIT Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1.INDUCTION Up-regulated in response to enterovirus 71 (EV71) infection.PTM Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases.SIMILARITY Belongs to the TFIIF alpha subunit family.CAUTION Was reported (PubMed:10428810) to have a protein kinase activity and to autophosphorylates on Ser-385 and Thr-389.
organism: Homo sapiens

UnificationXref90
type: UnificationXref
id: 113416
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=113416

UnificationXref92
type: UnificationXref
id: P52434
db: UniProt

UnificationXref91
type: UnificationXref
id: REACT_3445
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_3445.2

UnificationXref94
type: UnificationXref
id: REACT_3575
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_3575.2

UnificationXref93
type: UnificationXref
id: 63523
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=63523

UnificationXref219
type: UnificationXref
id: REACT_7442
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7442.2

UnificationXref217
type: UnificationXref
id: P69723
db: UniProt

UnificationXref218
type: UnificationXref
id: 175527
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175527

UnificationXref215
type: UnificationXref
id: 3004623
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3004623

UnificationXref216
type: UnificationXref
id: REACT_160982
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_160982.1

UnificationXref213
type: UnificationXref
id: REACT_7779
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7779.2

UnificationXref214
type: UnificationXref
id: P17096
db: UniProt

UnificationXref221
type: UnificationXref
id: 175251
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175251

UnificationXref220
type: UnificationXref
id: P04591
db: UniProt

UnificationXref223
type: UnificationXref
id: 3006250
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3006250

UnificationXref222
type: UnificationXref
id: REACT_7234
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7234.2

UnificationXref97
type: UnificationXref
id: REACT_2516
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_2516.2

UnificationXref98
type: UnificationXref
id: P61218
db: UniProt

UnificationXref95
type: UnificationXref
id: P53803
db: UniProt

UniProt:Q9UK41 VPS28
type: ProteinReference
name: VPS28
xref: UnificationXref369
comment: FUNCTION Component of the ESCRT-I complex, a regulator of vesicular trafficking process.SUBUNIT Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoechiometry. Interacts with TSG101, VPS37B, VPS37C, MVB12A and MVB12B. Interacts WITH VPS36; the interaction mediates the association with the ESCRT-II complex. Interacts with SNF8 and VPS25. Interacts with CEP55.SIMILARITY Belongs to the VPS28 family.SIMILARITY Contains 1 VPS28 C-terminal domain.SIMILARITY Contains 1 VPS28 N-terminal domain.
organism: Homo sapiens

UnificationXref96
type: UnificationXref
id: 63537
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=63537

http://identifiers.org/pubmed/2406030
type: PublicationXref
id: 2406030
author: Tiley, LS
author: Rusche, JR
author: Malim, MH
author: McCarn, DF
author: Hauber, J
author: Cullen, BR
db: Pubmed
title: HIV-1 structural gene expression requires binding of the Rev trans-activator to its RNA target sequence
source: Cell 60:675-83
year: 1990

UnificationXref99
type: UnificationXref
id: 83714
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=83714

UnificationXref228
type: UnificationXref
id: REACT_7432
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7432.2

UnificationXref229
type: UnificationXref
id: 175152
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175152

UnificationXref224
type: UnificationXref
id: REACT_161232
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_161232.1

UnificationXref225
type: UnificationXref
id: 175256
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175256

UnificationXref226
type: UnificationXref
id: REACT_7306
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7306.2

UnificationXref227
type: UnificationXref
id: 175200
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175200

http://identifiers.org/pubmed/2002555
type: PublicationXref
id: 2002555
author: Sodroski, J
author: Furman, C
author: Olshevsky, U
author: Helseth, E
db: Pubmed
title: Human immunodeficiency virus type 1 gp120 envelope glycoprotein regions important for association with the gp41 transmembrane glycoprotein
source: J Virol 65:2119-23
year: 1991

Human immunodeficiency virus 1
type: BioSource
xref: UnificationXref2

Homo sapiens
type: BioSource
xref: UnificationXref47

Synthesis and processing of ENV and VPU
type: Pathway
data source: Reactome
pathway component: Transport of trimeric ENV precursor to Golgi apparatus
pathway component: Transport of trimeric gp41:gp120 to plasma membrane
pathway component: Synthesis of VPU protein
pathway component: Transport of VPU to Plasma Membrane
pathway component: Glycosylation of ENV polyprotein
pathway component: Splicing of HIV RNA transcript
pathway component: Synthesis of ENV polyprotein
pathway component: Folding and Oligomerization of ENV glycoprotein
pathway component: Transport of VPU to Golgi apparatus
pathway component: Cleavage of the viral Env gp160 precursor polyprotein
xref: http://identifiers.org/pubmed/22762019
xref: UnificationXref73
xref: RelationshipXref3
xref: UnificationXref72
pathway order: PathwayStep3
pathway order: PathwayStep1
pathway order: PathwayStep2
pathway order: PathwayStep5
pathway order: PathwayStep4
pathway order: PathwayStep7
pathway order: PathwayStep10
pathway order: PathwayStep8
pathway order: PathwayStep9
pathway order: PathwayStep6
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: The two viral membrane proteins, Env and the accessory protein Vpu, which are encoded by the same mRNA, are translated on the rough ER. All virion components need to traffic from their point of synthesis to sites of assembly on the plasma membrane. Env is an integral membrane protein. It is inserted cotranslationally into ER membranes and then travels through the cellular secretory pathway where it is glycosylated, assembled into trimeric complexes, processed into the gp41 and gp120 subunits by the cellular protease furin.
comment: Edited: Gillespie, ME, 2013-01-29
comment: Authored: Gillespie, ME, 2013-03-06
organism: Human immunodeficiency virus 1

http://identifiers.org/pubmed/11112487
type: PublicationXref
id: 11112487
author: Coren, LV
author: Schubert, U
author: Gagliardi, TD
author: Ott, DE
author: Chertova, EN
db: Pubmed
title: Ubiquitination of HIV-1 and MuLV Gag
source: Virology 278:111-21
year: 2000

http://identifiers.org/pubmed/16175173
type: PublicationXref
id: 16175173
author: Lewinski, M
author: Hoffmann, C
author: Hannenhalli, S
author: Bushman, FD
author: Leipzig, J
author: Ciuffi, A
author: Barr, S
db: Pubmed
title: Genome-wide analysis of retroviral DNA integration
source: Nat Rev Microbiol 3:848-58
year: 2005

UniProt:P05919 vpu
type: ProteinReference
name: vpu
xref: UnificationXref59
comment: SUBUNIT May form pentamers or hexamers. Forms ternary complexes, by interacting with human CD4 and BTRC, and with human BST2 and BTRC.MISCELLANEOUS HIV-1 lineages are divided in three main groups, M (for Major), O (for Outlier), and N (for New, or Non-M, Non-O). The vast majority of strains found worldwide belong to the group M. Group O seems to be endemic to and largely confined to Cameroon and neighboring countries in West Central Africa, where these viruses represent a small minority of HIV-1 strains. The group N is represented by a limited number of isolates from Cameroonian persons. The group M is further subdivided in 9 clades or subtypes (A to D, F to H, J and K).SIMILARITY Belongs to the HIV-1 VPU protein family.
organism: Human immunodeficiency virus 1

POLR2J
type: ProteinReference
name: UniProt:P52435 POLR2J
name: POLR2J1
xref: UnificationXref117
comment: SUBUNIT Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. Interacts with AATF.TISSUE SPECIFICITY Ubiquitously expressed. High expression was found in heart and skeletal muscle.SIMILARITY Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.
organism: Homo sapiens

UniProt:Q8NEZ2 VPS37A
type: ProteinReference
name: VPS37A
name: HCRP1
xref: UnificationXref366
comment: FUNCTION Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.SUBUNIT Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoechiometry. Interacts with TSG101, VPS28 and HGS.TISSUE SPECIFICITY Widely expressed. Examined tissues include heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. More abundant in liver. Strongly decreased or undetected in hepatomas.DISEASE Spastic paraplegia autosomal recessive 53 (SPG53) [MIM:614898]: A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. Complicated forms are characterized by the addition of such neurological features as spastic quadriparesis, seizures, dementia, amyotrophy, extrapyramidal disturbance, cerebral or cerebellar atrophy, optic atrophy, and peripheral neuropathy, as well as by extra neurological manifestations such as dysmorphism, albinism, retinitis pigmentosa, deafness, dementia, amyotrophy and ichthyosis. SPG53 is characterized by pronounced early onset spastic paraparesis of upper and lower limbs, mild intellectual disability, kyphosis, pectus carinatum and hypertrichosis. Note=The disease is caused by mutations affecting the gene represented in this entry.SIMILARITY Belongs to the VPS37 family.SIMILARITY Contains 1 VPS37 C-terminal domain.
organism: Homo sapiens

DFS70
type: ProteinReference
name: UniProt:O75475 PSIP1
name: PSIP2
name: PSIP1
name: LEDGF
xref: UnificationXref209
comment: FUNCTION Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.SUBUNIT Monomer. Interacts with IFRD1/PC4. Isoform 2 interacts with SFRS1. Isoform 1 interacts POGZ and CDCA7L. Isoform 1 interacts with lentiviral integrase and acts as a chromatin tethering factor to the chromosomal DNA. Isoform 1 interacts in particular with the human HIV-1 integrase protein (HIV-1 IN), determining its nuclear localization, its tight association with chromatin and its protection from the proteasome. Isoform 1 interacts also with HIV-2 IN. Isoform 2 does not interact with HIV-1 IN.TISSUE SPECIFICITY Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.DOMAIN Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain).DISEASE Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.SIMILARITY Belongs to the HDGF family.SIMILARITY Contains 1 PWWP domain.
organism: Homo sapiens

UnificationXref403
type: UnificationXref
id: REACT_165036
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_165036.1

UnificationXref402
type: UnificationXref
id: 3149459
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149459

UnificationXref401
type: UnificationXref
id: REACT_164711
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164711.1

UnificationXref400
type: UnificationXref
id: 3149458
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149458

UnificationXref407
type: UnificationXref
id: REACT_164147
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164147.1

UnificationXref406
type: UnificationXref
id: 3149462
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149462

UnificationXref405
type: UnificationXref
id: REACT_165059
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_165059.1

http://identifiers.org/pubmed/10757782
type: PublicationXref
id: 10757782
author: Gaynor, RB
author: Ivanov, D
author: Kwak, YT
author: Guo, J
db: Pubmed
title: Domains in the SPT5 protein that modulate its transcriptional regulatory properties
source: Mol Cell Biol 20:2970-83
year: 2000

UnificationXref404
type: UnificationXref
id: 3149468
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149468

UnificationXref409
type: UnificationXref
id: REACT_164888
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164888.1

UnificationXref408
type: UnificationXref
id: 3149466
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149466

UnificationXref410
type: UnificationXref
id: 3149456
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149456

UnificationXref416
type: UnificationXref
id: 3149434
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149434

UnificationXref415
type: UnificationXref
id: REACT_165330
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_165330.1

UnificationXref418
type: UnificationXref
id: 174490
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=174490

UnificationXref417
type: UnificationXref
id: REACT_163872
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_163872.1

UnificationXref412
type: UnificationXref
id: 184380
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184380

UnificationXref411
type: UnificationXref
id: REACT_164785
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164785.1

UnificationXref414
type: UnificationXref
id: 3149449
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149449

UnificationXref413
type: UnificationXref
id: REACT_164477
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164477.1

UnificationXref419
type: UnificationXref
id: REACT_115893
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_115893.3

UnificationXref420
type: UnificationXref
id: 174495
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=174495

UnificationXref421
type: UnificationXref
id: REACT_115860
db: Reactome
id version: 5
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_115860.5

http://identifiers.org/pubmed/17129629
type: PublicationXref
id: 17129629
author: Welman, Mélanie
author: Lemay, Guy
author: Cohen, Eric A
db: Pubmed
title: Role of envelope processing and gp41 membrane spanning domain in the formation of human immunodeficiency virus type 1 (HIV-1) fusion-competent envelope glycoprotein complex
source: Virus Res. 124:103-12
year: 2007

POLR2H
type: ProteinReference
name: UniProt:P52434 POLR2H
xref: UnificationXref92
comment: FUNCTION DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.SUBUNIT Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively. Directly interacts with POLR2A.SIMILARITY Belongs to the eukaryotic RPB8 RNA polymerase subunit family.
organism: Homo sapiens

http://identifiers.org/pubmed/16728975
type: PublicationXref
id: 16728975
author: Roux, Kenneth H
author: Lifson, Jeffrey D
author: Taylor, Kenneth A
author: Ofek, Gilad A
author: Chertova, Elena
author: Liu, J
author: Zhu, Ping
author: Grisé, Henry
author: Bess, Julian
db: Pubmed
title: Distribution and three-dimensional structure of AIDS virus envelope spikes
source: Nature 441:847-52
year: 2006

http://identifiers.org/pubmed/16291214
type: PublicationXref
id: 16291214
author: Lewinski, MK
author: Bushman, FD
db: Pubmed
title: Retroviral DNA integration--mechanism and consequences
source: Adv Genet 55:147-81
year: 2005

SequenceSite12
type: SequenceSite
position status: EQUAL
sequence position: 961

SequenceSite11
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite14
type: SequenceSite
position status: EQUAL
sequence position: 150

SequenceSite13
type: SequenceSite
position status: EQUAL
sequence position: 2

SequenceSite10
type: SequenceSite
position status: EQUAL
sequence position: 372

SequenceSite19
type: SequenceSite
position status: EQUAL
sequence position: 5

SequenceSite16
type: SequenceSite
position status: EQUAL
sequence position: 58

SequenceSite15
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite18
type: SequenceSite
position status: EQUAL
sequence position: 127

SequenceSite17
type: SequenceSite
position status: EQUAL
sequence position: 1

UniProt:P17096 HMGA1
type: ProteinReference
name: HMGIY
name: HMGA1
xref: UnificationXref214
comment: FUNCTION HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.SUBUNIT Interacts with HIPK2 (By similarity). Interacts with HIV-1 pre-integration complex.PTM Constitutively phosphorylated on two or three sites. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity.PTM HMG-Y is not methylated.PTM Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.DISEASE Note=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B.SIMILARITY Belongs to the HMGA family.SIMILARITY Contains 3 A.T hook DNA-binding domains.
organism: Homo sapiens

POLR2K
type: ProteinReference
name: UniProt:P53803 POLR2K
xref: UnificationXref95
comment: FUNCTION DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.SIMILARITY Belongs to the archaeal RpoP/eukaryotic RPC10 RNA polymerase subunit family.
organism: Homo sapiens

http://identifiers.org/pubmed/15802467
type: PublicationXref
id: 15802467
author: Holman, AG
author: Coffin, JM
db: Pubmed
title: Symmetrical base preferences surrounding HIV-1, avian sarcoma/leukosis virus, and murine leukemia virus integration sites
source: Proc Natl Acad Sci U S A 102:6103-7
year: 2005

mitochondrial intermembrane space
type: CellularLocationVocabulary
xref: UnificationXref6

http://identifiers.org/pubmed/15509564
type: PublicationXref
id: 15509564
author: Bieniasz, PD
author: Martin-Serrano, J
author: Eastman, SW
author: Chung, W
author: Zang, T
db: Pubmed
title: Identification of human VPS37C, a component of endosomal sorting complex required for transport-I important for viral budding
source: J Biol Chem 280:628-36
year: 2005

PACE
type: ProteinReference
name: UniProt:P09958 FURIN
name: FUR
name: FURIN
name: PCSK3
xref: UnificationXref48
comment: FUNCTION Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.CATALYTIC ACTIVITY Release of mature proteins from their proproteins by cleavage of -Arg-Xaa-Yaa-Arg-|-Zaa- bonds, where Xaa can be any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and vWF from their respective precursors.COFACTOR Calcium.ENZYME REGULATION Could be inhibited by the not secondly cleaved propeptide.SUBUNIT Interacts with FLNA (By similarity). Binds to PACS1 which mediates TGN localization and connection to clathrin adapters.TISSUE SPECIFICITY Seems to be expressed ubiquitously.DOMAIN Contains a cytoplasmic domain responsible for its TGN localization and recycling from the cell surface.PTM The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation.PTM Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms.SIMILARITY Belongs to the peptidase S8 family. Furin subfamily.SIMILARITY Contains 1 homo B/P domain.
organism: Homo sapiens

HIV Transcription Termination
type: Pathway
data source: Reactome
xref: RelationshipXref9
xref: UnificationXref267
xref: UnificationXref268
comment: Edited: Matthews, L, 2005-07-26 23:29:22
comment: Termination of HIV-1 transcription is believed to be mechanistically the same as termination of endogenous human messages.
comment: Authored: Matthews, L, Rice, AP, 2005-07-27 00:00:00
organism: Human immunodeficiency virus 1

http://identifiers.org/pubmed/2556643
type: PublicationXref
id: 2556643
author: Green, MR
author: Zapp, ML
db: Pubmed
title: Sequence-specific RNA binding by the HIV-1 Rev protein
source: Nature 342:714-6
year: 1989

Reactome
type: Provenance
comment: http://www.reactome.org

http://identifiers.org/pubmed/16260736
type: PublicationXref
id: 16260736
author: Ambrosio, AL
author: Engelman, A
author: Ellenberger, T
author: Cherepanov, P
author: Rahman, S
db: Pubmed
title: Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75
source: Proc Natl Acad Sci U S A 102:17308-13
year: 2005

http://identifiers.org/pubmed/12663786
type: PublicationXref
id: 12663786
author: Bieniasz, PD
author: Martin-Serrano, J
author: Zang, T
db: Pubmed
title: Role of ESCRT-I in retroviral budding
source: J Virol 77:4794-804
year: 2003

RelationshipXref11
type: RelationshipXref
id: 184469
db: Reactome Database ID Release 45
relationship type: Same Catalyst Activity
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184469

RelationshipXref10
type: RelationshipXref
id: GO:0004379
db: GENE ONTOLOGY
relationship type: gene ontology term for cellular function

RelationshipXref12
type: RelationshipXref
id: GO:0008565
db: GENE ONTOLOGY
relationship type: gene ontology term for cellular function

RelationshipXref13
type: RelationshipXref
id: 3159230
db: Reactome Database ID Release 45
relationship type: Same Catalyst Activity
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3159230

RelationshipXref14
type: RelationshipXref
id: GO:0046788
db: GENE ONTOLOGY
relationship type: gene ontology term for cellular process

SequenceSite151
type: SequenceSite
position status: EQUAL
sequence position: 228

SequenceSite150
type: SequenceSite
position status: EQUAL
sequence position: 153

SequenceSite153
type: SequenceSite
position status: EQUAL
sequence position: 76

UnificationXref60
type: UnificationXref
id: 180550
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=180550

SequenceSite152
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref61
type: UnificationXref
id: REACT_9354
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9354.3

SequenceSite155
type: SequenceSite
position status: EQUAL
sequence position: 152

SequenceSite154
type: SequenceSite
position status: EQUAL
sequence position: 77

SequenceSite157
type: SequenceSite
position status: EQUAL
sequence position: 228

SequenceSite156
type: SequenceSite
position status: EQUAL
sequence position: 153

SequenceSite82
type: SequenceSite
position status: EQUAL
sequence position: 153

SequenceSite83
type: SequenceSite
position status: EQUAL
sequence position: 228

SequenceSite80
type: SequenceSite
position status: EQUAL
sequence position: 77

SequenceSite81
type: SequenceSite
position status: EQUAL
sequence position: 152

SequenceSite76
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite75
type: SequenceSite
position status: EQUAL
sequence position: 76

SequenceSite74
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite73
type: SequenceSite
position status: EQUAL
sequence position: 498

SequenceSite79
type: SequenceSite
position status: EQUAL
sequence position: 76

UnificationXref59
type: UnificationXref
id: P05919
db: UniProt

SequenceSite78
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite77
type: SequenceSite
position status: EQUAL
sequence position: 76

SequenceSite158
type: SequenceSite
position status: EQUAL
sequence position: 229

UnificationXref56
type: UnificationXref
id: REACT_164721
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164721.1

http://identifiers.org/pubmed/9557688
type: PublicationXref
id: 9557688
author: Hoffmann, C
author: Bushman, FD
author: Carteau, S
db: Pubmed
title: Chromosome structure and human immunodeficiency virus type 1 cDNA integration: centromeric alphoid repeats are a disfavored target
source: J Virol 72:4005-14
year: 1998

SequenceSite159
type: SequenceSite
position status: EQUAL
sequence position: 304

UnificationXref55
type: UnificationXref
id: 189840
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=189840

UnificationXref58
type: UnificationXref
id: REACT_163857
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_163857.1

UnificationXref57
type: UnificationXref
id: 173647
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=173647

UnificationXref52
type: UnificationXref
id: 171288
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=171288

http://identifiers.org/pubmed/16311605
type: PublicationXref
id: 16311605
author: Poeschla, E
author: Hoffmann, C
author: Llano, M
author: Ecker, JR
author: Bushman, FD
author: Shinn, P
author: Leipzig, J
author: Ciuffi, A
db: Pubmed
title: A role for LEDGF/p75 in targeting HIV DNA integration
source: Nat Med 11:1287-9
year: 2005

UnificationXref51
type: UnificationXref
id: MI:0355
db: MI

UnificationXref54
type: UnificationXref
id: GO:0005886
db: GENE ONTOLOGY

UnificationXref53
type: UnificationXref
id: REACT_163905
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_163905.1

SequenceSite164
type: SequenceSite
position status: EQUAL
sequence position: 457

SequenceSite163
type: SequenceSite
position status: EQUAL
sequence position: 456

UnificationXref50
type: UnificationXref
id: REACT_4414
db: Reactome
id version: 4
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_4414.4

SequenceSite162
type: SequenceSite
position status: EQUAL
sequence position: 381

SequenceSite161
type: SequenceSite
position status: EQUAL
sequence position: 380

SequenceSite168
type: SequenceSite
position status: EQUAL
sequence position: 609

SequenceSite167
type: SequenceSite
position status: EQUAL
sequence position: 608

SequenceSite166
type: SequenceSite
position status: EQUAL
sequence position: 533

SequenceSite165
type: SequenceSite
position status: EQUAL
sequence position: 532

SequenceSite160
type: SequenceSite
position status: EQUAL
sequence position: 305

SequenceSite70
type: SequenceSite
position status: EQUAL
sequence position: 488

SequenceSite71
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite72
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite63
type: SequenceSite
position status: EQUAL
sequence position: 2

SequenceSite62
type: SequenceSite
position status: EQUAL
sequence position: 107

SequenceSite65
type: SequenceSite
position status: EQUAL
sequence position: 2

SequenceSite64
type: SequenceSite
position status: EQUAL
sequence position: 132

rev
type: ProteinReference
name: UniProt:P04618 rev
xref: UnificationXref184
comment: FUNCTION Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. These pre-mRNAs carry a recognition sequence called Rev responsive element (RRE) located in the env gene, that is not present in fully spliced viral mRNAs (early transcripts). This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs. Rev itself is translated from a fully spliced mRNA that readily exits the nucleus. Rev's nuclear localization signal (NLS) binds directly to KPNB1/Importin beta-1 without previous binding to KPNA1/Importin alpha-1. KPNB1 binds to the GDP bound form of RAN (Ran-GDP) and targets Rev to the nucleus. In the nucleus, the conversion from Ran-GDP to Ran-GTP dissociates Rev from KPNB1 and allows Rev's binding to the RRE in viral pre-mRNAs. Rev multimerization on the RRE via cooperative assembly exposes its nuclear export signal (NES) to the surface. Rev can then form a complex with XPO1/CRM1 and Ran-GTP, leading to nuclear export of the complex. Conversion from Ran-GTP to Ran-GDP mediates dissociation of the Rev/RRE/XPO1/RAN complex, so that Rev can return to the nucleus for a subsequent round of export. Beside KPNB1, also seems to interact with TNPO1/Transportin-1, RANBP5/IPO5 and IPO7/RANBP7 for nuclear import. The nucleoporin-like HRB/RIP is an essential cofactor that probably indirectly interacts with Rev to release HIV RNAs from the perinuclear region to the cytoplasm (By similarity). Interacts with DDX1; the interaction is necessary for proper subcellular localization of this protein.PTM Phosphorylated by protein kinase CK2. Presence of, and maybe binding to the N-terminus of the regulatory beta subunit of CK2 is necessary for CK2-mediated Rev's phosphorylation.MISCELLANEOUS HIV-1 lineages are divided in three main groups, M (for Major), O (for Outlier), and N (for New, or Non-M…
organism: Human immunodeficiency virus 1

SequenceSite67
type: SequenceSite
position status: EQUAL
sequence position: 449

SequenceSite66
type: SequenceSite
position status: EQUAL
sequence position: 132

UnificationXref49
type: UnificationXref
id: 157049
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=157049

SequenceSite69
type: SequenceSite
position status: EQUAL
sequence position: 441

UnificationXref48
type: UnificationXref
id: P09958
db: UniProt

SequenceSite68
type: SequenceSite
position status: EQUAL
sequence position: 500

A-152E5.7
type: ProteinReference
name: POLR2C
name: UniProt:P19387 POLR2C
xref: UnificationXref108
comment: SUBUNIT Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB11/POLR2J and RPB3/POLR2C subunits interact with each other.SIMILARITY Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.
organism: Homo sapiens

UnificationXref47
type: UnificationXref
id: 9606
db: NCBI Taxonomy

UnificationXref46
type: UnificationXref
id: REACT_165266
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_165266.1

SequenceSite169
type: SequenceSite
position status: EQUAL
sequence position: 684

UnificationXref45
type: UnificationXref
id: 189281
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=189281

POLR2E
type: ProteinReference
name: UniProt:P19388 POLR2E
xref: UnificationXref105
comment: SUBUNIT Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively (By similarity). In RNA Pol II, this subunit is present in 2-fold molar excess over the other subunits. Interacts with URI1. Interacts with HBV protein X.SIMILARITY Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family.
organism: Homo sapiens

UnificationXref44
type: UnificationXref
id: REACT_164426
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164426.1

UnificationXref43
type: UnificationXref
id: 189315
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=189315

UnificationXref42
type: UnificationXref
id: REACT_164136
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164136.1

UnificationXref41
type: UnificationXref
id: 189359
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=189359

UnificationXref40
type: UnificationXref
id: REACT_164340
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164340.1

SequenceSite133
type: SequenceSite
position status: EQUAL
sequence position: 251

SequenceSite132
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite135
type: SequenceSite
position status: EQUAL
sequence position: 397

SequenceSite134
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref80
type: UnificationXref
id: 51801
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=51801

UnificationXref81
type: UnificationXref
id: REACT_3988
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_3988.2

SequenceSite131
type: SequenceSite
position status: EQUAL
sequence position: 390

UnificationXref82
type: UnificationXref
id: 167183
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=167183

UniProt:P62487 POLR2G
type: ProteinReference
name: POLR2G
name: RPB7
xref: UnificationXref120
comment: FUNCTION DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA.SUBUNIT Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex.SIMILARITY Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.
organism: Homo sapiens

SequenceSite130
type: SequenceSite
position status: EQUAL
sequence position: 2

UnificationXref83
type: UnificationXref
id: REACT_6577
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6577.2

UniProt:P04608 tat
type: ProteinReference
name: tat
xref: UnificationXref84
comment: SUBUNIT Interacts with host CCNT1. Associates with the P-TEFb complex composed at least of Tat, P-TEFb (CDK9 and CCNT1), TAR RNA, RNA Pol II. Recruits the HATs CREBBP, TAF1/TFIID, EP300, PCAF and GCN5L2. Interacts with host KAT5/Tip60; this interaction targets the latter to degradation. Interacts with the host deacetylase SIRT1. Interacts with host capping enzyme RNGTT; this interaction stimulates RNGTT. Binds to host KDR, and to the host integrins ITGAV/ITGB3 and ITGA5/ITGB1. Interacts with host KPNB1/importin beta-1 without previous binding to KPNA1/importin alpha-1. Interacts with EIF2AK2. Interacts with host nucleosome assembly protein NAP1L1; this interaction may be required for the transport of Tat within the nucleus, since the two proteins interact at the nuclear rim. Interacts with host C1QBP/SF2P32; this interaction involves lysine-acetylated Tat. Interacts with the host chemokine receptors CCR2, CCR3 and CXCR4. Interacts with host DPP4/CD26; this interaction may trigger an anti-proliferative effect. Interacts with host LDLR. Interacts with the host extracellular matrix metalloproteinase MMP1. Interacts with host PRMT6; this interaction mediates Tat's methylation. Interacts with, and is ubiquitinated by MDM2/Hdm2. Interacts with host PSMC3 and HTATIP2. Interacts with STAB1; this interaction may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T cells by binding to the same domain than HDAC1 (By similarity). Interacts (when acetylated on Lys-50 and Lys-51) with human CDK13, thereby increasing HIV-1 mRNA splicing and promoting the production of the doubly spliced HIV-1 protein Nef.MISCELLANEOUS This truncated variant has a premature stop codon. It may have arose as a consequence of tissue culture passaging.MISCELLANEOUS HIV-1 lineages are divided in three main groups, M (for Major), O (for Outlier), and N (for New, or Non-M, Non-O). The vast majority of strains found worldwide belong to the group M. Group O seems to be endemic to and large…
organism: Human immunodeficiency virus 1

SequenceSite99
type: SequenceSite
position status: EQUAL
sequence position: 608

SequenceSite98
type: SequenceSite
position status: EQUAL
sequence position: 533

SequenceSite97
type: SequenceSite
position status: EQUAL
sequence position: 532

SequenceSite96
type: SequenceSite
position status: EQUAL
sequence position: 457

SequenceSite95
type: SequenceSite
position status: EQUAL
sequence position: 456

Catalysis5
type: Catalysis
data source: Reactome
controlled: Monoubiquitinated N-myristoyl GAG polyprotein is targeted to the late endosomal vesicle membrane by the ESCRT-I complex
control type: ACTIVATION
xref: RelationshipXref12
xref: RelationshipXref13
controller: ESCRT-I

UnificationXref74
type: UnificationXref
id: MI:0359
db: MI

Catalysis6
type: Catalysis
data source: Reactome
controlled: Transport of GAG to the Plasma Membrane
control type: ACTIVATION
xref: RelationshipXref12
xref: RelationshipXref13
controller: ESCRT-I

UnificationXref73
type: UnificationXref
id: REACT_163660
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_163660.1

UnificationXref76
type: UnificationXref
id: O60563
db: UniProt

UnificationXref75
type: UnificationXref
id: GO:0005654
db: GENE ONTOLOGY

SequenceSite136
type: SequenceSite
position status: EQUAL
sequence position: 1

Catalysis1
type: Catalysis
data source: Reactome
controlled: Cleavage of the viral Env gp160 precursor polyprotein
control type: ACTIVATION
xref: RelationshipXref2
xref: RelationshipXref1
controller: Furin

UnificationXref78
type: UnificationXref
id: REACT_2892
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_2892.2

SequenceSite137
type: SequenceSite
position status: EQUAL
sequence position: 221

UnificationXref77
type: UnificationXref
id: 51611
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=51611

Catalysis2
type: Catalysis
data source: Reactome
controlled: Phosphorylation of DSIF by the P-TEFb(Cyclin T1:Cdk9) complex
control type: ACTIVATION
xref: RelationshipXref5
xref: RelationshipXref4
controller: Tat-containing early elongation complex with hyperphosphorylated Pol II CTD and phospho-NELF

SequenceSite138
type: SequenceSite
position status: EQUAL
sequence position: 1

Catalysis3
type: Catalysis
data source: Reactome
controlled: Transesterification to connect viral DNA 3' ends to host DNA 5' ends
control type: ACTIVATION
xref: RelationshipXref7
xref: RelationshipXref6
controller: IN:viral DNA bound to host genomic DNA with staggered ends

SequenceSite139
type: SequenceSite
position status: EQUAL
sequence position: 285

UnificationXref79
type: UnificationXref
id: P50750
db: UniProt

Catalysis4
type: Catalysis
data source: Reactome
controlled: N-myristoylation of GAG polyprotein by NMT2
control type: ACTIVATION
xref: RelationshipXref10
xref: RelationshipXref11
controller: N-myristoyltransferase 2

SequenceSite146
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite145
type: SequenceSite
position status: EQUAL
sequence position: 76

UnificationXref391
type: UnificationXref
id: REACT_164792
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164792.1

SequenceSite144
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref390
type: UnificationXref
id: 3149455
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149455

SequenceSite143
type: SequenceSite
position status: EQUAL
sequence position: 76

SequenceSite142
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref71
type: UnificationXref
id: REACT_163863
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_163863.1

ADP [ChEBI:16761]
type: SmallMoleculeReference
name: ADP
name: Adenosine 5'-diphosphate
xref: UnificationXref171

SequenceSite141
type: SequenceSite
position status: EQUAL
sequence position: 355

UnificationXref72
type: UnificationXref
id: 171286
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=171286

SequenceSite140
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref70
type: UnificationXref
id: 3149432
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149432

UnificationXref397
type: UnificationXref
id: REACT_165426
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_165426.1

SequenceSite91
type: SequenceSite
position status: EQUAL
sequence position: 304

UnificationXref396
type: UnificationXref
id: 3149467
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149467

SequenceSite92
type: SequenceSite
position status: EQUAL
sequence position: 305

POLR2L
type: ProteinReference
name: UniProt:P62875 POLR2L
xref: UnificationXref126
comment: SIMILARITY Belongs to the archaeal RpoN/eukaryotic RPB10 RNA polymerase subunit family.
organism: Homo sapiens

UnificationXref399
type: UnificationXref
id: REACT_164423
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164423.1

SequenceSite93
type: SequenceSite
position status: EQUAL
sequence position: 380

UnificationXref398
type: UnificationXref
id: 3149464
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149464

SequenceSite94
type: SequenceSite
position status: EQUAL
sequence position: 381

UnificationXref393
type: UnificationXref
id: REACT_165448
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_165448.1

UnificationXref392
type: UnificationXref
id: 3149469
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149469

UnificationXref395
type: UnificationXref
id: REACT_165285
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_165285.1

UnificationXref394
type: UnificationXref
id: 3149465
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149465

SequenceSite90
type: SequenceSite
position status: EQUAL
sequence position: 229

SequenceSite89
type: SequenceSite
position status: EQUAL
sequence position: 228

UnificationXref198
type: UnificationXref
id: P04585
db: UniProt

SequenceSite88
type: SequenceSite
position status: EQUAL
sequence position: 153

UnificationXref199
type: UnificationXref
id: 175288
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175288

SequenceSite85
type: SequenceSite
position status: EQUAL
sequence position: 76

UnificationXref194
type: UnificationXref
id: REACT_6228
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6228.3

SequenceSite84
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref195
type: UnificationXref
id: O75531
db: UniProt

SequenceSite87
type: SequenceSite
position status: EQUAL
sequence position: 152

UnificationXref196
type: UnificationXref
id: 175030
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175030

SequenceSite86
type: SequenceSite
position status: EQUAL
sequence position: 77

UnificationXref197
type: UnificationXref
id: REACT_7027
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7027.2

UnificationXref65
type: UnificationXref
id: REACT_164984
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164984.1

UnificationXref190
type: UnificationXref
id: REACT_6379
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6379.3

UnificationXref64
type: UnificationXref
id: 3149439
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149439

UnificationXref191
type: UnificationXref
id: 165532
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=165532

UnificationXref63
type: UnificationXref
id: REACT_163666
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_163666.1

UnificationXref192
type: UnificationXref
id: REACT_6517
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6517.2

UnificationXref62
type: UnificationXref
id: 3149433
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149433

UnificationXref193
type: UnificationXref
id: 165033
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=165033

http://identifiers.org/pubmed/1549584
type: PublicationXref
id: 1549584
author: Syu, W J
author: Wolf, A
author: Du, B
author: Essex, M
author: Matsuda, M
author: Lee, T H
author: Tan, S
author: Lee, W R
db: Pubmed
title: Nonrandom distribution of gp120 N-linked glycosylation sites important for infectivity of human immunodeficiency virus type 1
source: Proc. Natl. Acad. Sci. U.S.A. 89:2213-7
year: 1992

SequenceSite149
type: SequenceSite
position status: EQUAL
sequence position: 152

UnificationXref69
type: UnificationXref
id: REACT_164892
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164892.1

UnificationXref68
type: UnificationXref
id: 3149423
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149423

SequenceSite147
type: SequenceSite
position status: EQUAL
sequence position: 76

UnificationXref67
type: UnificationXref
id: REACT_163732
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_163732.1

SequenceSite148
type: SequenceSite
position status: EQUAL
sequence position: 77

UnificationXref66
type: UnificationXref
id: 3149440
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149440

endoplasmic reticulum membrane
type: CellularLocationVocabulary
xref: UnificationXref18

SequenceSite39
type: SequenceSite
position status: EQUAL
sequence position: 2

UnificationXref11
type: UnificationXref
id: AF033819
db: EMBL

UnificationXref12
type: UnificationXref
id: 187209
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=187209

SequenceSite37
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref13
type: UnificationXref
id: REACT_9274
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_9274.2

SUPT4H
type: ProteinReference
name: UniProt:P63272 SUPT4H1
name: SUPT4H1
name: SPT4H
xref: UnificationXref158
comment: FUNCTION Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.SUBUNIT Interacts with SUPT5H to form DSIF. DSIF interacts with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of CDK9 and cyclin-T (CCNT1 or CCNT2). DSIF interacts with RNA polymerase II, and this interaction is reduced by phosphorylation of the C-terminal domain (CTD) of POLR2A by P-TEFb. DSIF also interacts with the NELF complex, which is composed of WHSC2/NELFA, COBRA1/NELFB, TH1L/NELFD and RDBP/NELFE, and this interaction occurs following prior binding of DSIF to RNA polymerase II. DSIF also interacts with HRMT1L2/PRMT1, HTATSF1/TATSF1, RNGTT/CAP1A, SKB1/PRMT5, SUPT6H, and can interact with PIN1.TISSUE SPECIFICITY Widely expresse…
organism: Homo sapiens

SequenceSite38
type: SequenceSite
position status: EQUAL
sequence position: 67

UnificationXref14
type: UnificationXref
id: 187215
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=187215

UnificationXref182
type: UnificationXref
id: 165533
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=165533

UnificationXref181
type: UnificationXref
id: REACT_6251
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6251.3

UnificationXref180
type: UnificationXref
id: 167235
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=167235

UnificationXref10
type: UnificationXref
id: REACT_7969
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7969.1

SequenceSite31
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref19
type: UnificationXref
id: P04578
db: UniProt

UnificationXref186
type: UnificationXref
id: REACT_7459
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_7459.2

SequenceSite32
type: SequenceSite
position status: EQUAL
sequence position: 117

UnificationXref185
type: UnificationXref
id: 175380
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=175380

UnificationXref184
type: UnificationXref
id: P04618
db: UniProt

SequenceSite30
type: SequenceSite
position status: EQUAL
sequence position: 125

UnificationXref183
type: UnificationXref
id: REACT_6408
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6408.3

SequenceSite35
type: SequenceSite
position status: EQUAL
sequence position: 2

UnificationXref15
type: UnificationXref
id: REACT_164050
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164050.1

SequenceSite36
type: SequenceSite
position status: EQUAL
sequence position: 142

UnificationXref16
type: UnificationXref
id: 187211
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=187211

UnificationXref189
type: UnificationXref
id: 165542
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=165542

SequenceSite33
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref17
type: UnificationXref
id: REACT_163953
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_163953.1

UnificationXref188
type: UnificationXref
id: REACT_6419
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6419.2

SequenceSite34
type: SequenceSite
position status: EQUAL
sequence position: 172

UnificationXref18
type: UnificationXref
id: GO:0005789
db: GENE ONTOLOGY

UnificationXref187
type: UnificationXref
id: 165535
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=165535

UnificationXref8
type: UnificationXref
id: REACT_8975
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_8975.2

UnificationXref9
type: UnificationXref
id: 180855
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=180855

UnificationXref4
type: UnificationXref
id: 173193
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=173193

UnificationXref5
type: UnificationXref
id: REACT_8060
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_8060.2

UnificationXref6
type: UnificationXref
id: GO:0005758
db: GENE ONTOLOGY

UnificationXref7
type: UnificationXref
id: 180888
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=180888

SequenceSite26
type: SequenceSite
position status: EQUAL
sequence position: 275

UnificationXref1
type: UnificationXref
id: GO:0005829
db: GENE ONTOLOGY

SequenceSite27
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite28
type: SequenceSite
position status: EQUAL
sequence position: 1174

UnificationXref3
type: UnificationXref
id: P69726
db: UniProt

SequenceSite29
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref2
type: UnificationXref
id: 11676
db: NCBI Taxonomy

UnificationXref171
type: UnificationXref
id: CHEBI:16761
db: ChEBI

UnificationXref170
type: UnificationXref
id: REACT_5903
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_5903.1

UnificationXref173
type: UnificationXref
id: REACT_3850
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_3850.1

UnificationXref172
type: UnificationXref
id: 113582
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=113582

SequenceSite20
type: SequenceSite
position status: EQUAL
sequence position: 2

UnificationXref175
type: UnificationXref
id: REACT_6628
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6628.1

SequenceSite21
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref174
type: UnificationXref
id: 170705
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=170705

SequenceSite22
type: SequenceSite
position status: EQUAL
sequence position: 1970

UnificationXref177
type: UnificationXref
id: REACT_6633
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6633.2

SequenceSite23
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref176
type: UnificationXref
id: 170710
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=170710

SequenceSite24
type: SequenceSite
position status: EQUAL
sequence position: 210

UnificationXref179
type: UnificationXref
id: REACT_6316
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6316.2

SequenceSite25
type: SequenceSite
position status: EQUAL
sequence position: 2

UnificationXref178
type: UnificationXref
id: 170704
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=170704

SequenceSite61
type: SequenceSite
position status: EQUAL
sequence position: 2

SequenceSite60
type: SequenceSite
position status: EQUAL
sequence position: 530

UnificationXref169
type: UnificationXref
id: 29358
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=29358

POLR2
type: ProteinReference
name: UniProt:P24928 POLR2A
name: POLR2A
xref: UnificationXref101
comment: FUNCTION DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.CATALYTIC ACTIVITY Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SUBUNIT Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. The phosphorylated C-terminal domain interacts with FNBP3 and SYNCRIP. Interacts with SAFB/SAFB1. Interacts with CCNL1 and MYO1C (By similarity). Interacts with CCNL2 and SFRS19. …
organism: Homo sapiens

UnificationXref160
type: UnificationXref
id: REACT_5720
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_5720.2

UnificationXref30
type: UnificationXref
id: GO:0000139
db: GENE ONTOLOGY

UnificationXref31
type: UnificationXref
id: 3149443
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149443

UnificationXref32
type: UnificationXref
id: REACT_165340
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_165340.1

UnificationXref33
type: UnificationXref
id: 3149446
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149446

UnificationXref34
type: UnificationXref
id: REACT_164089
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164089.1

UnificationXref35
type: UnificationXref
id: 174491
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=174491

SequenceSite59
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref36
type: UnificationXref
id: REACT_163888
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_163888.1

http://identifiers.org/pubmed/16775314
type: PublicationXref
id: 16775314
author: Habermann, A
author: Krausslich, HG
author: Jager, S
author: Gottwein, E
db: Pubmed
title: Cumulative mutations of ubiquitin acceptor sites in human immunodeficiency virus type 1 gag cause a late budding defect
source: J Virol 80:6267-75
year: 2006

UnificationXref37
type: UnificationXref
id: 189269
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=189269

UnificationXref168
type: UnificationXref
id: CHEBI:15422
db: ChEBI

SequenceSite57
type: SequenceSite
position status: EQUAL
sequence position: 1148

UnificationXref38
type: UnificationXref
id: REACT_164192
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164192.1

UnificationXref167
type: UnificationXref
id: REACT_6495
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6495.2

SequenceSite58
type: SequenceSite
position status: EQUAL
sequence position: 1435

UnificationXref39
type: UnificationXref
id: 189280
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=189280

UnificationXref166
type: UnificationXref
id: 170707
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=170707

SequenceSite55
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref165
type: UnificationXref
id: REACT_2797
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_2797.3

SequenceSite56
type: SequenceSite
position status: EQUAL
sequence position: 89

SequenceSite53
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref164
type: UnificationXref
id: 112420
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=112420

SequenceSite54
type: SequenceSite
position status: EQUAL
sequence position: 1087

UnificationXref163
type: UnificationXref
id: REACT_160383
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_160383.1

SequenceSite51
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref162
type: UnificationXref
id: 3009372
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3009372

SequenceSite52
type: SequenceSite
position status: EQUAL
sequence position: 117

UnificationXref161
type: UnificationXref
id: O00267
db: UniProt

P/OKcl.15
type: ProteinReference
name: NELFA
name: UniProt:Q9H3P2 NELFA
name: WHSC2
xref: UnificationXref144
comment: FUNCTION Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex.SUBUNIT The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE. Interacts with the RNA polymerase II complex when it is not phosphorylated by P-TEFb.TISSUE SPECIFICITY Ubiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney.DOMAIN The HDAg-like domain is essential for transcriptional repression, and mediates the interaction with the RNA polymerase II complex.SIMILARITY Belongs to the NELF-A family.SIMILARITY Contains 1 HDAg-like domain.CAUTION PubMed:12612062 has shown that it is not involved in Wolf-Hirschhorn syndrome.
organism: Homo sapiens

SequenceSite50
type: SequenceSite
position status: EQUAL
sequence position: 380

UnificationXref158
type: UnificationXref
id: P63272
db: UniProt

UnificationXref159
type: UnificationXref
id: 64905
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=64905

UnificationXref20
type: UnificationXref
id: 189343
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=189343

UnificationXref21
type: UnificationXref
id: REACT_165112
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_165112.1

UnificationXref24
type: UnificationXref
id: 174493
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=174493

SequenceSite48
type: SequenceSite
position status: EQUAL
sequence position: 580

UnificationXref25
type: UnificationXref
id: REACT_163798
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_163798.1

SequenceSite49
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref22
type: UnificationXref
id: 174494
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=174494

UnificationXref23
type: UnificationXref
id: REACT_163787
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_163787.1

SequenceSite44
type: SequenceSite
position status: EQUAL
sequence position: 590

UnificationXref28
type: UnificationXref
id: 171291
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=171291

UnificationXref155
type: UnificationXref
id: REACT_6596
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6596.1

UnificationXref29
type: UnificationXref
id: REACT_163952
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_163952.1

SequenceSite45
type: SequenceSite
position status: EQUAL
sequence position: 2

UnificationXref154
type: UnificationXref
id: 170709
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=170709

UnificationXref26
type: UnificationXref
id: 189843
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=189843

SequenceSite46
type: SequenceSite
position status: EQUAL
sequence position: 528

UnificationXref157
type: UnificationXref
id: REACT_6586
db: Reactome
id version: 4
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6586.4

UnificationXref27
type: UnificationXref
id: REACT_165165
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_165165.1

SequenceSite47
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref156
type: UnificationXref
id: 167074
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=167074

SequenceSite40
type: SequenceSite
position status: EQUAL
sequence position: 249

UnificationXref151
type: UnificationXref
id: MOD:00696
db: MOD

SequenceSite41
type: SequenceSite
position status: EQUAL
sequence position: 2

UnificationXref150
type: UnificationXref
id: P18615
db: UniProt

SequenceSite42
type: SequenceSite
position status: EQUAL
sequence position: 517

UnificationXref153
type: UnificationXref
id: REACT_6460
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_6460.2

SequenceSite43
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref152
type: UnificationXref
id: 170698
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=170698

VPS37C
type: Protein
entity reference: PML39
data source: Reactome
xref: UnificationXref376
xref: UnificationXref377
feature: FragmentFeature69
cellular location: endosome membrane
comment: Reactome DB_ID: 184438

UnificationXref134
type: UnificationXref
id: P35269
db: UniProt

VPS37B
type: Protein
entity reference: UniProt:Q9H9H4 VPS37B
data source: Reactome
xref: UnificationXref373
xref: UnificationXref374
feature: FragmentFeature68
cellular location: endosome membrane
comment: Reactome DB_ID: 184293

UnificationXref135
type: UnificationXref
id: 65565
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=65565

VPS28
type: Protein
entity reference: UniProt:Q9UK41 VPS28
data source: Reactome
xref: UnificationXref371
xref: UnificationXref370
feature: FragmentFeature67
cellular location: endosome membrane
comment: Reactome DB_ID: 184342

UnificationXref338
type: UnificationXref
id: 939187
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939187

UnificationXref132
type: UnificationXref
id: 65567
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=65567

VPS37A
type: Protein
entity reference: UniProt:Q8NEZ2 VPS37A
data source: Reactome
xref: UnificationXref367
xref: UnificationXref368
feature: FragmentFeature66
cellular location: endosome membrane
comment: Reactome DB_ID: 184333

UnificationXref339
type: UnificationXref
id: REACT_27662
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27662.2

UnificationXref133
type: UnificationXref
id: REACT_5029
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_5029.2

VPS37D
type: Protein
entity reference: WBSCR24
data source: Reactome
xref: UnificationXref364
xref: UnificationXref365
feature: FragmentFeature65
cellular location: endosome membrane
comment: Reactome DB_ID: 184289

UnificationXref336
type: UnificationXref
id: 939247
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939247

UnificationXref130
type: UnificationXref
id: REACT_4889
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_4889.1

Tumor susceptibility gene 101 protein
type: Protein
entity reference: UniProt:Q99816 TSG101
data source: Reactome
name: TSG101(2-390)
name: TS101_HUMAN
xref: UnificationXref362
xref: UnificationXref361
feature: FragmentFeature64
cellular location: endosome membrane
comment: Reactome DB_ID: 2980549
display name: TSG101

UnificationXref337
type: UnificationXref
id: REACT_27644
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27644.2

UnificationXref131
type: UnificationXref
id: P13984
db: UniProt

ubiquitin (UBC 9)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref355
xref: UnificationXref354
feature: FragmentFeature63
cellular location: endosome membrane
comment: Reactome DB_ID: 939237
display name: UBC(609-684)

UnificationXref334
type: UnificationXref
id: 939251
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939251

ubiquitin (UBC 8)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref352
xref: UnificationXref353
feature: FragmentFeature62
cellular location: endosome membrane
comment: Reactome DB_ID: 939221
display name: UBC(533-608)

UnificationXref335
type: UnificationXref
id: REACT_27803
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27803.2

ubiquitin
type: Protein
data source: Reactome
member physical entity: ubiquitin (UBC 2)
member physical entity: ubiquitin (UBB 3)
member physical entity: ubiquitin (UBC 4)
member physical entity: ubiquitin (UBC 8)
member physical entity: ubiquitin (UBB 1)
member physical entity: ubiquitin (UBC 7)
member physical entity: ubiquitin (UBC 9)
member physical entity: ubiquitin (UBA52)
member physical entity: ubiquitin (RPS27A)
member physical entity: ubiquitin (UBC 3)
member physical entity: ubiquitin (UBB 2)
member physical entity: ubiquitin (UBC 5)
member physical entity: ubiquitin (UBC 1)
member physical entity: ubiquitin (UBC 6)
xref: UnificationXref410
xref: UnificationXref411
cellular location: plasma membrane
comment: Converted from EntitySet in Reactome
comment: Reactome DB_ID: 3149456

ubiquitin (RPS27A)
type: Protein
entity reference: UBA80
data source: Reactome
xref: UnificationXref382
xref: UnificationXref383
feature: FragmentFeature70
cellular location: plasma membrane
comment: Reactome DB_ID: 3149457
display name: RPS27A(1-76)

UnificationXref333
type: UnificationXref
id: REACT_27953
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27953.2

ubiquitin (UBA52)
type: Protein
entity reference: UniProt:P62987 UBA52
data source: Reactome
xref: UnificationXref384
xref: UnificationXref385
feature: FragmentFeature71
cellular location: plasma membrane
comment: Reactome DB_ID: 3149452
display name: UBA52(1-76)

UnificationXref332
type: UnificationXref
id: 939240
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939240

http://identifiers.org/pubmed/15308744
type: PublicationXref
id: 15308744
author: Poeschla, EM
author: Peretz, M
author: Llano, M
author: Vanegas, M
author: Chung, S
author: Fregoso, O
author: Saenz, D
db: Pubmed
title: LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration complexes
source: J Virol 78:9524-37
year: 2004

UnificationXref331
type: UnificationXref
id: REACT_27824
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27824.2

UnificationXref330
type: UnificationXref
id: 939189
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939189

UnificationXref139
type: UnificationXref
id: 113425
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=113425

UnificationXref138
type: UnificationXref
id: REACT_4708
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_4708.1

UnificationXref137
type: UnificationXref
id: 109631
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=109631

UnificationXref136
type: UnificationXref
id: REACT_4868
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_4868.2

UnificationXref349
type: UnificationXref
id: REACT_27588
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27588.2

ubiquitin (UBC 2)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref340
xref: UnificationXref341
feature: FragmentFeature56
cellular location: endosome membrane
comment: Reactome DB_ID: 939161
display name: UBC(77-152)

UnificationXref143
type: UnificationXref
id: REACT_4038
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_4038.3

phosphorylated residue
type: SequenceModificationVocabulary
xref: UnificationXref151

UBC(1-76)
type: Protein
entity reference: UBC
data source: Reactome
name: ubiquitin (UBC 1)
xref: UnificationXref339
xref: UnificationXref338
feature: FragmentFeature55
cellular location: endosome membrane
comment: Reactome DB_ID: 939187
display name: UBC

UnificationXref144
type: UnificationXref
id: Q9H3P2
db: UniProt

ubiquitin (UBC 4)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref345
xref: UnificationXref344
feature: FragmentFeature58
cellular location: endosome membrane
comment: Reactome DB_ID: 939172
display name: UBC(229-304)

UnificationXref145
type: UnificationXref
id: 112426
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=112426

ubiquitin (UBC 3)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref342
xref: UnificationXref343
feature: FragmentFeature57
cellular location: endosome membrane
comment: Reactome DB_ID: 939170
display name: UBC(153-228)

UnificationXref146
type: UnificationXref
id: REACT_2992
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_2992.3

UnificationXref345
type: UnificationXref
id: REACT_27324
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27324.2

UBB(1-76)
type: Protein
entity reference: UniProt:P0CG47 UBB
data source: Reactome
name: ubiquitin (UBB 1)
xref: UnificationXref333
xref: UnificationXref332
feature: FragmentFeature52
cellular location: endosome membrane
comment: Reactome DB_ID: 939240
display name: UBB

UnificationXref346
type: UnificationXref
id: 939196
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939196

UBA52(1-76)
type: Protein
entity reference: UniProt:P62987 UBA52
data source: Reactome
name: ubiquitin (UBA52)
xref: UnificationXref330
xref: UnificationXref331
feature: FragmentFeature51
cellular location: endosome membrane
comment: Reactome DB_ID: 939189
display name: UBA52

UnificationXref140
type: UnificationXref
id: REACT_3841
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_3841.1

UnificationXref347
type: UnificationXref
id: REACT_27321
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27321.2

ubiquitin (UBB 3)
type: Protein
entity reference: UniProt:P0CG47 UBB
data source: Reactome
xref: UnificationXref337
xref: UnificationXref336
feature: FragmentFeature54
cellular location: endosome membrane
comment: Reactome DB_ID: 939247
display name: UBB(153-228)

UnificationXref141
type: UnificationXref
id: Q8IXH7
db: UniProt

http://identifiers.org/pubmed/7956067
type: PublicationXref
id: 7956067
author: Pryciak, PM
author: Muller, HP
author: Varmus, HE
db: Pubmed
title: Retroviral integration machinery as a probe for DNA structure and associated proteins
source: Cold Spring Harb Symp Quant Biol 58:533-41
year: 1993

UnificationXref348
type: UnificationXref
id: 939166
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939166

ubiquitin (UBB 2)
type: Protein
entity reference: UniProt:P0CG47 UBB
data source: Reactome
xref: UnificationXref335
xref: UnificationXref334
feature: FragmentFeature53
cellular location: endosome membrane
comment: Reactome DB_ID: 939251
display name: UBB(77-152)

UnificationXref142
type: UnificationXref
id: 112428
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=112428

ubiquitin (UBC 6)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref349
xref: UnificationXref348
feature: FragmentFeature60
cellular location: endosome membrane
comment: Reactome DB_ID: 939166
display name: UBC(381-456)

ubiquitin (UBC 5)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref347
xref: UnificationXref346
feature: FragmentFeature59
cellular location: endosome membrane
comment: Reactome DB_ID: 939196
display name: UBC(305-380)

UnificationXref342
type: UnificationXref
id: 939170
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939170

UnificationXref341
type: UnificationXref
id: REACT_27835
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27835.2

http://identifiers.org/pubmed/1317268
type: PublicationXref
id: 1317268
author: Pryciak, PM
author: Varmus, HE
db: Pubmed
title: Nucleosomes, DNA-binding proteins, and DNA sequence modulate retroviral integration target site selection
source: Cell 69:769-80
year: 1992

UnificationXref344
type: UnificationXref
id: 939172
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939172

ubiquitin (UBC 7)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref350
xref: UnificationXref351
feature: FragmentFeature61
cellular location: endosome membrane
comment: Reactome DB_ID: 939199
display name: UBC(457-532)

UnificationXref343
type: UnificationXref
id: REACT_27708
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27708.2

UnificationXref148
type: UnificationXref
id: 112427
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=112427

UniProt:Q9Y5B0 CTDP1
type: ProteinReference
name: CTDP1
name: FCP1
xref: UnificationXref89
comment: FUNCTION Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M-phase-promoting factor (MPF)/CDK1 inactivation.CATALYTIC ACTIVITY A phosphoprotein + H(2)O = a protein + phosphate.SUBUNIT Homodimer. Interacts with GTF2F1. Interacts with WDR77, SNRPB and SNRNP70.TISSUE SPECIFICITY Ubiquitously expressed. Isoform 3 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and placenta.PTM Phosphorylated. In the presence of TFIIF, the phosphorylated form has an increased CTD phosphatase activity. The phosphorylation is required for the physical interaction with GTF2F1.DISEASE Congenital cataracts, facial dysmorphism, and neuropathy (CCFDN) [MIM:604168]: An autosomal recessive developmental disorder characterized by a complex clinical phenotype with seemingly unrelated features involving multiple organs and systems. Developmental abnormalities include congenital cataracts and microcorneae, hypomyelination of the peripheral nervous system, impaired physical growth, delayed early motor and intellectual development, facial dysmorphism and hypogonadism. Central nervous system involvement, with cerebral and spinal cord atrophy, may be the result of disrupted development with superimposed degenerative changes. Affected individuals are prone to severe rhabdomyolysis after viral infections and to serious complications related to general anesthesia (such as pulmonary edema and epileptic seizures). Note=The disease is caused by mutations affecting the gene represented in this entry.SIMILARITY Contains 1 BRCT domain.SIMILARITY Contains 1 FCP1 homology domain.
organism: Homo sapiens

UnificationXref147
type: UnificationXref
id: Q8WX92
db: UniProt

UnificationXref340
type: UnificationXref
id: 939161
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939161

UnificationXref149
type: UnificationXref
id: REACT_2263
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_2263.3

UnificationXref314
type: UnificationXref
id: REACT_24496
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24496.2

SequenceSite4
type: SequenceSite
position status: EQUAL
sequence position: 856

UnificationXref315
type: UnificationXref
id: 939239
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939239

SequenceSite5
type: SequenceSite
position status: EQUAL
sequence position: 108

UnificationXref312
type: UnificationXref
id: REACT_24153
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24153.2

SequenceSite6
type: SequenceSite
position status: EQUAL
sequence position: 794

UnificationXref313
type: UnificationXref
id: 939184
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939184

SequenceSite7
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref318
type: UnificationXref
id: REACT_24885
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24885.2

UnificationXref112
type: UnificationXref
id: 63504
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=63504

SequenceSite8
type: SequenceSite
position status: EQUAL
sequence position: 726

UnificationXref319
type: UnificationXref
id: 113595
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=113595

UnificationXref113
type: UnificationXref
id: REACT_4642
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_4642.2

SequenceSite9
type: SequenceSite
position status: EQUAL
sequence position: 1

UnificationXref316
type: UnificationXref
id: REACT_24411
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24411.2

UnificationXref110
type: UnificationXref
id: REACT_2538
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_2538.2

UnificationXref317
type: UnificationXref
id: 939165
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939165

UnificationXref111
type: UnificationXref
id: P30876
db: UniProt

CCNT1
type: ProteinReference
name: UniProt:O60563 CCNT1
xref: UnificationXref76
comment: FUNCTION Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.SUBUNIT Cyclin-T1 is the predominant cyclin that associates with CDK9 to form a heterodimer called P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Interacts with the transactivation region of HIV-1, HIV-2 and SIV Tat. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with MDFIC.TISSUE SPECIFICITY Ubiquitously expressed.MISCELLANEOUS Interaction between Tat and cyclin-T1 requires zinc.SIMILARITY Belongs to the cyclin family. Cyclin C subfamily.
organism: Homo sapiens

UnificationXref117
type: UnificationXref
id: P52435
db: UniProt

UnificationXref116
type: UnificationXref
id: REACT_2931
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_2931.2

UnificationXref115
type: UnificationXref
id: 63525
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=63525

UnificationXref114
type: UnificationXref
id: P36954
db: UniProt

UnificationXref311
type: UnificationXref
id: 939191
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939191

SequenceSite1
type: SequenceSite
position status: EQUAL
sequence position: 33

UnificationXref310
type: UnificationXref
id: REACT_24675
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24675.2

UnificationXref119
type: UnificationXref
id: REACT_2381
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_2381.2

SequenceSite3
type: SequenceSite
position status: EQUAL
sequence position: 512

UnificationXref118
type: UnificationXref
id: 63531
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=63531

SequenceSite2
type: SequenceSite
position status: EQUAL
sequence position: 511

ubiquitin (UBB 2)
type: Protein
entity reference: UniProt:P0CG47 UBB
data source: Reactome
xref: UnificationXref389
xref: UnificationXref388
feature: FragmentFeature73
cellular location: plasma membrane
comment: Reactome DB_ID: 3149463
display name: UBB(77-152)

UnificationXref323
type: UnificationXref
id: 184323
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184323

ubiquitin (UBB 1)
type: Protein
entity reference: UniProt:P0CG47 UBB
data source: Reactome
xref: UnificationXref386
xref: UnificationXref387
feature: FragmentFeature72
cellular location: plasma membrane
comment: Reactome DB_ID: 3149453
display name: UBB(1-76)

UnificationXref324
type: UnificationXref
id: REACT_115708
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_115708.2

ubiquitin (UBC 1)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref392
xref: UnificationXref393
feature: FragmentFeature75
cellular location: plasma membrane
comment: Reactome DB_ID: 3149469
display name: UBC(1-76)

UnificationXref325
type: UnificationXref
id: GO:0010008
db: GENE ONTOLOGY

ubiquitin (UBB 3)
type: Protein
entity reference: UniProt:P0CG47 UBB
data source: Reactome
xref: UnificationXref391
xref: UnificationXref390
feature: FragmentFeature74
cellular location: plasma membrane
comment: Reactome DB_ID: 3149455
display name: UBB(153-228)

UnificationXref326
type: UnificationXref
id: 184361
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184361

UnificationXref120
type: UnificationXref
id: P62487
db: UniProt

ubiquitin (UBC 3)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref397
xref: UnificationXref396
feature: FragmentFeature77
cellular location: plasma membrane
comment: Reactome DB_ID: 3149467
display name: UBC(153-228)

UnificationXref327
type: UnificationXref
id: REACT_116460
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_116460.2

UnificationXref121
type: UnificationXref
id: 63517
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=63517

ubiquitin (UBC 2)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref394
xref: UnificationXref395
feature: FragmentFeature76
cellular location: plasma membrane
comment: Reactome DB_ID: 3149465
display name: UBC(77-152)

UnificationXref328
type: UnificationXref
id: 939238
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939238

UnificationXref122
type: UnificationXref
id: REACT_2758
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_2758.2

ubiquitin (UBC 5)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref401
xref: UnificationXref400
feature: FragmentFeature79
cellular location: plasma membrane
comment: Reactome DB_ID: 3149458
display name: UBC(305-380)

UnificationXref329
type: UnificationXref
id: REACT_27897
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27897.2

UnificationXref123
type: UnificationXref
id: O15514
db: UniProt

ubiquitin (UBC 4)
type: Protein
entity reference: UBC
data source: Reactome
xref: UnificationXref398
xref: UnificationXref399
feature: FragmentFeature78
cellular location: plasma membrane
comment: Reactome DB_ID: 3149464
display name: UBC(229-304)

UnificationXref124
type: UnificationXref
id: 63508
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=63508

UnificationXref126
type: UnificationXref
id: P62875
db: UniProt

UnificationXref125
type: UnificationXref
id: REACT_5494
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_5494.2

UnificationXref128
type: UnificationXref
id: REACT_5392
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_5392.2

UnificationXref127
type: UnificationXref
id: 83715
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=83715

UnificationXref320
type: UnificationXref
id: REACT_3316
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_3316.3

UnificationXref129
type: UnificationXref
id: 109909
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=109909

UnificationXref322
type: UnificationXref
id: REACT_116777
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_116777.1

UnificationXref321
type: UnificationXref
id: 184369
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184369

SequenceSite129
type: SequenceSite
position status: EQUAL
sequence position: 684

UniProt:O75531 BANF1
type: ProteinReference
name: BAF
name: BCRG1
name: BANF1
xref: UnificationXref195
comment: FUNCTION Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.SUBUNIT Homodimer. Heterodimerizes with BAFL. Interacts with ANKLE2/LEM4, leading to decreased phosphorylation by VRK1 and promoting dephosphorylation by protein phosphatase 2A (PP2A). Binds non-specifically to double-stranded DNA, and is found as a hexamer or dodecamer upon DNA binding. Binds to LEM domain-containing nuclear proteins such as LEMD3/MAN1, TMPO/LAP2 and EMD (emerin). Interacts with CRX and LMNA (lamin-A). Binds linker histone H1.1 and core histones H3 with in vitro affinities of 500-900 and 100-200 nM. Interacts with HIV-1 pre-integration complex in cytoplasm by binding to viral matrix protein and Gag polyprotein.TISSUE SPECIFICITY Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.DOMAIN Has a helix-hairpin-helix (HhH) structural motif conserved among proteins that bind non-specifically to DNA.DOMAIN LEM domain proteins bind centrally on the BAF dimer, whereas DNA binds to the left and right sides.PTM Ser-4 is the major site of phosphorylation as compared to Thr-2 and Thr-3. Phosphorylation on Thr-2; Thr-3 and Ser-4 disrupts its ability to bind DNA and reduces its ability to b…
organism: Homo sapiens

SequenceSite126
type: SequenceSite
position status: EQUAL
sequence position: 533

SequenceSite125
type: SequenceSite
position status: EQUAL
sequence position: 532

SequenceSite128
type: SequenceSite
position status: EQUAL
sequence position: 609

SequenceSite127
type: SequenceSite
position status: EQUAL
sequence position: 608

Synthesis of VPU protein
type: BiochemicalReaction
data source: Reactome
xref: UnificationXref63
xref: UnificationXref62
left: Spliced Env mRNA
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: The VPU protein is produced
comment: Edited: Gillespie, ME, 2013-05-23
comment: Authored: Gillespie, ME, 2013-03-06
right: VPU (P05919)

UnificationXref379
type: UnificationXref
id: REACT_27580
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27580.1

Cleavage of the viral Env gp160 precursor polyprotein
type: BiochemicalReaction
data source: Reactome
EC number: 3.4.21
xref: UnificationXref53
xref: UnificationXref52
xref: http://identifiers.org/pubmed/8163529
left: Trimeric ENV precursor
comment: The trimeric gp160 complexes are cleaved into the gp41 and gp120 subunits by the cellular protease furin.
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: Edited: Gillespie, ME, 2013-05-23
comment: Authored: Gopinathrao, G, 2006-02-09 20:02:24
right: Trimeric gp120:gp41 oligomer

UnificationXref378
type: UnificationXref
id: 184398
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184398

Transport of trimeric gp41:gp120 to plasma membrane
type: BiochemicalReaction
data source: Reactome
xref: UnificationXref58
xref: http://identifiers.org/pubmed/2002555
xref: UnificationXref57
left: Trimeric gp120:gp41 oligomer
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: The cleaved and assembled gp41:gp121 complexes are transport to teh plasma membrane. This complex ultimately arrives via the cellular secretion pathway. Env is an integral membrane protein shuttled through the ER and Golgi where it was glycosylated and cleaved into the gp41 and gp120 subunits. The trimeric complex is brought to the plasma membrane by the host vesicular transport system. Only 7-14 trimers are present per virion.
comment: Edited: Gillespie, ME, 2013-05-23
comment: Authored: Gillespie, ME, 2013-03-06
right: Trimeric gp120:gp41 oligomer

UnificationXref376
type: UnificationXref
id: 184438
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184438

Transport of trimeric ENV precursor to Golgi apparatus
type: BiochemicalReaction
data source: Reactome
xref: UnificationXref36
xref: UnificationXref35
xref: http://identifiers.org/pubmed/17129629
left: Trimeric ENV precursor
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: The trimeric ENV precursor complex is transported from the ER to the Golgi.
comment: Edited: Gillespie, ME, 2013-05-23
comment: Authored: Gillespie, ME, 2013-03-06
right: Trimeric ENV precursor

UnificationXref377
type: UnificationXref
id: REACT_27780
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27780.1

Folding and Oligomerization of ENV glycoprotein
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/16728975
xref: UnificationXref29
xref: UnificationXref28
participant stoichiometry: Stoichiometry1
left: Envelope glycoprotein gp160
comment: The monomeric GP160 ENV precursor protein assembles into a trimer.
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: Edited: Gillespie, ME, 2013-05-23
comment: Authored: Gopinathrao, G, 2006-04-02 11:51:26
right: Trimeric ENV precursor

UnificationXref374
type: UnificationXref
id: REACT_27802
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27802.1

Glycosylation of ENV polyprotein
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/1549584
xref: UnificationXref25
xref: UnificationXref24
left: Envelope glycoprotein gp160
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: There are numerous N-linked glycosylation sites that are important for infectivity of human immunodeficiency virus type 1. With more than 20 consensus N-linked glycosylation sites in gp120 it is expected that a number are important for virion function.
comment: Edited: Gillespie, ME, 2013-05-23
comment: Authored: Gillespie, ME, 2013-03-06
right: Envelope glycoprotein gp160

UnificationXref375
type: UnificationXref
id: A5D8V6
db: UniProt

Synthesis of ENV polyprotein
type: BiochemicalReaction
data source: Reactome
xref: UnificationXref22
xref: UnificationXref23
left: Spliced Env mRNA
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: Edited: Gillespie, ME, 2013-05-23
comment: The ENV precursor protein gp160 is synthesized.
comment: Authored: Gillespie, ME, 2013-03-06
right: Envelope glycoprotein gp160

UnificationXref372
type: UnificationXref
id: Q9H9H4
db: UniProt

Splicing of HIV RNA transcript
type: BiochemicalReaction
data source: Reactome
xref: UnificationXref17
xref: http://identifiers.org/pubmed/20082634
xref: UnificationXref16
left: HIV-1 unspliced RNA
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: Edited: Gillespie, ME, 2013-01-29
comment: Authored: Gillespie, ME, 2013-03-06
comment: HIV is characterized by the production of multiple-spliced RNA species. The genomic fragmant is processesed creating multiple mRNA fragments.
right: Spliced Env mRNA

UnificationXref373
type: UnificationXref
id: 184293
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184293

Trimeric ENV precursor
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry2
component: Envelope glycoprotein gp160
xref: UnificationXref26
xref: UnificationXref27
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 189843

Translocation of Vpr to the mitochondria
type: BiochemicalReaction
data source: Reactome
xref: UnificationXref10
xref: UnificationXref9
xref: http://identifiers.org/pubmed/10620603
left: VPR (P69726) protein
comment: Authored: Matthews, L, 2006-05-15 07:16:46
comment: Edited: Matthews, L, 2006-06-02 10:55:30
comment: Reviewed: Zhao, RY, 2006-07-11 22:08:52
comment: Vpr translocates to the mitochondria.
right: VPR (P69726) protein

UnificationXref370
type: UnificationXref
id: 184342
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184342

Trimeric ENV precursor
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry3
component: Glycosylated Envelope glycoprotein gp160
xref: UnificationXref33
xref: UnificationXref34
cellular location: Golgi membrane
comment: Reactome DB_ID: 3149446

EMBL:AF033819
type: RnaReference
name: EMBL:AF033819 EMBL:AF033819
xref: UnificationXref11
organism: Human immunodeficiency virus 1

UnificationXref371
type: UnificationXref
id: REACT_27654
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27654.1

Trimeric gp120:gp41 oligomer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry7
component stoichiometry: Stoichiometry5
component: gp41 homotrimer
component: gp120 homotrimer
xref: UnificationXref46
xref: UnificationXref45
cellular location: cytosol
comment: Reactome DB_ID: 189281

SequenceSite121
type: SequenceSite
position status: EQUAL
sequence position: 380

SequenceSite122
type: SequenceSite
position status: EQUAL
sequence position: 381

SequenceSite123
type: SequenceSite
position status: EQUAL
sequence position: 456

SequenceSite124
type: SequenceSite
position status: EQUAL
sequence position: 457

SequenceSite120
type: SequenceSite
position status: EQUAL
sequence position: 305

Phosphorylation of DSIF by the P-TEFb(Cyclin T1:Cdk9) complex
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/10757782
xref: UnificationXref178
xref: UnificationXref179
left: Adenosine 5'-triphosphate
left: Tat-containing early elongation complex with hyperphosphorylated Pol II CTD and phospho-NELF
comment: Phosphorylation of the Spt5 subunit of DSIF by P-TEFb(Cyclin T1:Cdk9) results in the conversion of DSIF to an elongation factor (Ivanov al. 2000).
comment: Authored: Matthews, L, Rice, AP, 2005-07-27 00:00:00
comment: Edited: Matthews, L, 2006-01-10 20:59:19
right: Adenosine 5'-diphosphate
right: Tat-containing early elongation complex with hyperphosphorylated Pol II CTD ( phospho-NELF phospho DSIF)

N-myristoylglycine
type: SequenceModificationVocabulary
xref: UnificationXref279

Formation of the P-TEFb(Cyclin T1:Cdk9) complex
type: BiochemicalReaction
data source: Reactome
xref: UnificationXref180
xref: UnificationXref181
xref: http://identifiers.org/pubmed/9356449
xref: http://identifiers.org/pubmed/9491887
left: CycT1
left: C-2K
comment: Tat associates with the Cyclin T1 subunit of the cellular kinase complex TAK/P-TEFb and recruits this complex to the TAR sequence in the HIV-1 RNA (Wei et al. 1998). This association between TAT, TAR and TAK/P-TEFb is believed to bring the catalytic subunit of TAK/P-TEFb in close proximity to Pol II where it hyperphosphorylates the CTD of Pol II .
right: P-TEFb(Cyclin T1:Cdk9) complex

SequenceSite119
type: SequenceSite
position status: EQUAL
sequence position: 304

Multimerization of Rev
type: BiochemicalReaction
data source: Reactome
xref: UnificationXref194
xref: http://identifiers.org/pubmed/2015625
xref: UnificationXref193
left: Rev-bound HIV-1 mRNA
left: Rev-multimer
comment: Reviewed: Kumar, A, 2007-01-31 22:47:49
comment: Edited: Matthews, L, 2005-07-26 23:04:06
comment: In order for Rev to function, multiple molecules must bind sequentiallly to the RRE (Malim and Cullen 1991).
comment: Authored: Matthews, L, Rice, AP, 2005-07-27 00:00:00
right: Rev multimer-bound HIV-1 mRNA

SequenceSite118
type: SequenceSite
position status: EQUAL
sequence position: 229

Target DNA binding
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/12202041
xref: http://identifiers.org/pubmed/1760846
xref: http://identifiers.org/pubmed/9557688
xref: http://identifiers.org/pubmed/16311605
xref: http://identifiers.org/pubmed/16291214
xref: UnificationXref245
xref: PublicationXref18
xref: http://identifiers.org/pubmed/15802467
xref: UnificationXref246
xref: http://identifiers.org/pubmed/15677323
xref: http://identifiers.org/pubmed/16175173
xref: http://identifiers.org/pubmed/15314653
xref: http://identifiers.org/pubmed/16260736
left: Host genomic DNA
left: IN bound to sticky 3' ends of viral DNA in PIC
comment: How the PIC finds favored sites on target DNA has not been fully clarified. Active genes are favored for integration, and favored sequences at the site of integration also influence the reaction. Studies of cells depeleted in PSIP1/LEDGF/p75 suggest that this protein acts as a tethering factor binding HIV PICs near integration target DNA. Access of PICs to sites on chromosomes may be significant, since centromeric alphoid repeats are disfavored for integration, perhaps due to wrapping in compact centromeric heterochromatin. Nucleosomes bound to the integration template also affect target site selection and integration complex binding.
comment: Edited: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22
comment: Reviewed: Bushman, FD, 2006-10-30 22:19:13
comment: Authored: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22
right: IN:viral DNA bound to host genomic DNA with staggered ends
right: viral PIC proteins

SequenceSite117
type: SequenceSite
position status: EQUAL
sequence position: 228

Transesterification to connect viral DNA 3' ends to host DNA 5' ends
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/1329090
xref: http://identifiers.org/pubmed/15308744
xref: PublicationXref28
xref: http://identifiers.org/pubmed/9557688
xref: http://identifiers.org/pubmed/7956067
xref: http://identifiers.org/pubmed/1310932
xref: UnificationXref251
xref: PublicationXref18
xref: UnificationXref252
xref: http://identifiers.org/pubmed/8883604
xref: http://identifiers.org/pubmed/12407101
xref: http://identifiers.org/pubmed/15677323
xref: http://identifiers.org/pubmed/1317268
xref: http://identifiers.org/pubmed/16260736
left: IN:viral DNA bound to host genomic DNA with staggered ends
left: viral PIC proteins
comment: Edited: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22
comment: Reviewed: Bushman, FD, 2006-10-30 22:19:13
comment: Authored: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22
comment: The first chemical step of integration involves a single step transesterification, in which the recessed 3' hydroxyl of the viral DNA becomes covalently joined to a protruding 5' end in the target DNA. This step at the same time cleaves the target DNA.
right: Integration intermediate
right: viral PIC proteins

SequenceSite116
type: SequenceSite
position status: EQUAL
sequence position: 153

Gap repair completes provirus integration
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/1760846
xref: http://identifiers.org/pubmed/16291214
xref: UnificationXref261
xref: UnificationXref262
xref: http://identifiers.org/pubmed/16175173
xref: PublicationXref18
participant stoichiometry: Stoichiometry83
left: Integration intermediate
left: viral PIC proteins
comment: The mechanism by which the integration reaction is completed has not been fully clarified. Unfolding of the integration intermediate resulting from the IN-catalyzed transesterification produces a branched DNA molecule. Denaturation of the host DNA between the points of joining produces DNA gaps at each host-virus DNA junction. How these gaps are repaired is unclear. Well studied host cell gap repair enzymes can carry out this repair step on model virus-host DNA junctions in vitro, providing candidate enzymes. However, efforts to show importance in vivo are complicated by the fact that the functions are either redundant or lethal when mutated. Because the strand transfer complex formed at the completion of integration is quite stable, there may be a requirement for a disassembly step to remove integrase and potentially other proteins to allow access of the gap repair machinery. In order to complete the last stages of integration, the viral proteins must be removed, and the gaps at the host virus DNA junctions repaired. The sequence in which the dissembly of PIC occus is not yet understood.
comment: Edited: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22
comment: Reviewed: Bushman, FD, 2006-10-30 22:19:13
comment: Authored: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22
right: Integrated provirus
right: IN (Integrase) (P04585) protein
right: viral PIC proteins

SequenceSite115
type: SequenceSite
position status: EQUAL
sequence position: 152

Rev molecules assemble onto the RRE RNA sequence through their ARM sequence
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/2556643
xref: http://identifiers.org/pubmed/2406030
xref: UnificationXref266
xref: UnificationXref265
left: REV (P04618) protein
left: HIV-1 mRNA
comment: Edited: Matthews, L, 2005-07-26 23:29:39
comment: Nuclear export of the unspliced and partially spliced HIV-1 transcripts requires the association of the HIV-1 Rev protein with a cis-acting RNA sequence known as the Rev Response Element (RRE) located within the env gene. The RRE forms a stem loop structure that associates with an arginine-rich RNA binding motif (ARM) within Rev.
comment: Reviewed: Kumar, A, 2007-01-31 22:47:49
comment: Authored: Matthews, L, Rice, AP, 2005-07-27 00:00:00
right: Rev-bound HIV-1 mRNA

SequenceSite114
type: SequenceSite
position status: EQUAL
sequence position: 77

Synthesis of GAG polyprotein
type: BiochemicalReaction
data source: Reactome
xref: UnificationXref271
xref: UnificationXref272
xref: PublicationXref38
left: HIV-1 unspliced RNA
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: Authored: Gillespie, ME, 2013-03-06
comment: Gag is translated from the unspliced viral RNA on free ribosomes in the cytoplasm. The products of the pro and pol genes are also synthesized from the unspliced viral RNA, but never as parts of an independent polyprotein. They are initially contained within the Gag-Pro or Gag-Pro-Pol fusion protein, the product of translational readthrough
right: GAG Polyprotein (P04591)

UnificationXref100
type: UnificationXref
id: REACT_3193
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_3193.2

Tat-containing early elongation complex with hyperphosphorylated Pol II CTD and phospho-NELF
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry15
component stoichiometry: Stoichiometry14
component stoichiometry: Stoichiometry32
component stoichiometry: Stoichiometry37
component stoichiometry: Stoichiometry41
component stoichiometry: Stoichiometry38
component: Tat:P-TEFb(Cyclin T1:Cdk9) complex
component: phospho-NELF complex
component: FCP1P protein
component: RNA Polymerase II holoenzyme complex (hyperphosphorylated):TFIIF complex
component: DSIF complex
component: HIV-1 template:capped HIV-1 transcript hybrid
xref: UnificationXref166
xref: UnificationXref167
cellular location: nucleoplasm
comment: Reactome DB_ID: 170707

Trimeric gp120:gp41 oligomer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry9
component stoichiometry: Stoichiometry8
component: gp41 homotrimer
component: gp120 homotrimer
xref: UnificationXref56
xref: UnificationXref55
cellular location: plasma membrane
comment: Reactome DB_ID: 189840

UnificationXref102
type: UnificationXref
id: MOD:00046
db: MOD

gp41 homotrimer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry6
component: Transmembrane protein gp41 (P04578)
xref: UnificationXref44
xref: UnificationXref43
cellular location: cytosol
comment: Reactome DB_ID: 189315

UnificationXref101
type: UnificationXref
id: P24928
db: UniProt

gp120 homotrimer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry4
component: Surface protein gp120 (P04578)
xref: UnificationXref39
xref: UnificationXref40
cellular location: cytosol
comment: Reactome DB_ID: 189280

UnificationXref389
type: UnificationXref
id: REACT_164681
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164681.1

Transport of VPU to Golgi apparatus
type: BiochemicalReaction
data source: Reactome
xref: UnificationXref66
xref: http://identifiers.org/pubmed/22553496
xref: UnificationXref67
left: VPU (P05919)
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: VPU is shuttled through the ER:Golgi protein expression pathway.
comment: Edited: Gillespie, ME, 2013-05-23
comment: Authored: Gillespie, ME, 2013-03-06
right: VPU (P05919)

P-TEFb(Cyclin T1:Cdk9) complex
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry10
component stoichiometry: Stoichiometry11
component: CycT1
component: C-2K
xref: UnificationXref82
xref: UnificationXref83
cellular location: nucleoplasm
comment: Reactome DB_ID: 167183

Transport of VPU to Plasma Membrane
type: BiochemicalReaction
data source: Reactome
xref: UnificationXref71
xref: UnificationXref70
xref: http://identifiers.org/pubmed/22553496
left: VPU (P05919)
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: Edited: Gillespie, ME, 2013-05-23
comment: Authored: Gillespie, ME, 2013-03-06
comment: Once transported to the plasma membrane the VPU protein will be incorporated into the assembling virus. The Vpu accessory protein is found to be required for efficient virion release from some cell lines but completely dispensible in others.
right: VPU (P05919)

Tat:P-TEFb(Cyclin T1:Cdk9) complex
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry13
component stoichiometry: Stoichiometry12
component: Tat (P04608)
component: P-TEFb(Cyclin T1:Cdk9) complex
xref: UnificationXref88
xref: UnificationXref87
cellular location: nucleoplasm
comment: Reactome DB_ID: 167237

UnificationXref385
type: UnificationXref
id: REACT_164033
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164033.1

UnificationXref107
type: UnificationXref
id: REACT_5583
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_5583.2

UnificationXref386
type: UnificationXref
id: 3149453
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149453

UnificationXref108
type: UnificationXref
id: P19387
db: UniProt

UnificationXref387
type: UnificationXref
id: REACT_165137
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_165137.1

UnificationXref109
type: UnificationXref
id: 63506
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=63506

UnificationXref388
type: UnificationXref
id: 3149463
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149463

UnificationXref381
type: UnificationXref
id: REACT_115855
db: Reactome
id version: 3
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_115855.3

UnificationXref103
type: UnificationXref
id: 113420
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=113420

UnificationXref382
type: UnificationXref
id: 3149457
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149457

UnificationXref104
type: UnificationXref
id: REACT_3260
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_3260.2

UnificationXref383
type: UnificationXref
id: REACT_164837
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_164837.1

UnificationXref105
type: UnificationXref
id: P19388
db: UniProt

UnificationXref384
type: UnificationXref
id: 3149452
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=3149452

UnificationXref106
type: UnificationXref
id: 83713
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=83713

SequenceSite112
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite113
type: SequenceSite
position status: EQUAL
sequence position: 76

SequenceSite110
type: SequenceSite
position status: EQUAL
sequence position: 153

UnificationXref380
type: UnificationXref
id: 184269
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184269

SequenceSite111
type: SequenceSite
position status: EQUAL
sequence position: 228

SequenceSite104
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite103
type: SequenceSite
position status: EQUAL
sequence position: 76

SequenceSite106
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite105
type: SequenceSite
position status: EQUAL
sequence position: 76

SequenceSite108
type: SequenceSite
position status: EQUAL
sequence position: 77

SequenceSite107
type: SequenceSite
position status: EQUAL
sequence position: 76

Stoichiometry9
type: Stoichiometry
physical entity: gp41 homotrimer
stoichiometric coefficient: 3.0

Transport of GAG to the Plasma Membrane
type: BiochemicalReaction
data source: Reactome
xref: UnificationXref416
xref: http://identifiers.org/pubmed/21289126
xref: UnificationXref417
left: monoubiquitinated N-myristoyl GAG (P04591) protein
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: Authored: Gillespie, ME, 2013-03-06
comment: Assembling Gag molecules are largely derived from the rapidly diffusing cytoplasmic pool. Gag membrane targeting requires myristoylation and a subset of GAG molecules are shuttled to the plasma membrane in this way.
right: monoubiquitinated N-myristoyl GAG (P04591) protein

UniProt:P13984 GTF2F2
type: ProteinReference
name: GTF2F2
name: RAP30
xref: UnificationXref131
comment: FUNCTION TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.CATALYTIC ACTIVITY ATP + H(2)O = ADP + phosphate.SUBUNIT Heterodimer of an alpha and a beta subunit. Interacts with HTATSF1 and GPBP1 (By similarity). Interacts with URI1.SIMILARITY Belongs to the TFIIF beta subunit family.
organism: Homo sapiens

SequenceSite109
type: SequenceSite
position status: EQUAL
sequence position: 152

UnificationXref359
type: UnificationXref
id: REACT_116878
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_116878.1

Monoubiquitination of N-myristoyl GAG polyprotein
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/9525617
xref: UnificationXref324
xref: http://identifiers.org/pubmed/11112487
xref: http://identifiers.org/pubmed/16775314
xref: UnificationXref323
left: ubiquitin
left: N-myristoyl GAG (P04591) protein
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: Cytosolic N-myristoyl Gag polyprotein is conjugated with a single molecule of ubiquitin. Conjugation is typically to one of two lysine residues in the p6 domain of Gag but can be to lysine residues in the MA, CA, NC, and SP2 domains of the protein. The specific host cell E2 and E3 proteins that mediate Gag ubiquitination have not been identified. The same studies that first identified the p6 ubiquitination sites in Gag also called the biological significance of Gag ubiquitination into question by demonstrating that Gag proteins in which the p6 ubiquitination sites had been removed by mutagenesis could still assemble efficiently into infectious viral particles (Ott et al. 1998, 2000). More recent work, however, has identified additional ubiquitination sites throughout the carboxyterminal region of the Gag polyprotein, and when all of these sites are removed by mutagenesis, both viral assembly involving the mutant Gag polyprotein and infectivity of the resulting viral particles are sharply reduced (Gottwein et al. 2006).
comment: Authored: Gillespie, ME, 2013-03-06
right: monoubiquitinated N-myristoyl GAG (P04591) protein

Stoichiometry6
type: Stoichiometry
physical entity: Transmembrane protein gp41 (P04578)
stoichiometric coefficient: 3.0

UnificationXref358
type: UnificationXref
id: 184475
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184475

Monoubiquitinated N-myristoyl GAG polyprotein is targeted to the late endosomal vesicle membrane by the ESCRT-I complex
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/15509564
xref: UnificationXref381
xref: UnificationXref380
xref: http://identifiers.org/pubmed/12663786
xref: http://identifiers.org/pubmed/15218037
left: monoubiquitinated N-myristoyl GAG (P04591) protein
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: Monoubiquitinated N-myristoyl Gag polyprotein associates with the ESCRT-1 complex at an endosomal membrane (Eastman et al. 2005; Martin-Serrano et al. 2003; Stuchell et al. 2004).
comment: Authored: Gillespie, ME, 2013-03-06
right: monoubiquitinated N-myristoyl GAG (P04591) protein

Stoichiometry5
type: Stoichiometry
physical entity: gp120 homotrimer
stoichiometric coefficient: 3.0

UnificationXref357
type: UnificationXref
id: REACT_27496
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27496.1

Stoichiometry8
type: Stoichiometry
physical entity: gp120 homotrimer
stoichiometric coefficient: 3.0

UnificationXref356
type: UnificationXref
id: 184258
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184258

Stoichiometry7
type: Stoichiometry
physical entity: gp41 homotrimer
stoichiometric coefficient: 3.0

N-myristoylation of GAG polyprotein by NMT2
type: BiochemicalReaction
data source: Reactome
EC number: 2.3.1.97
xref: http://identifiers.org/pubmed/9506952
xref: http://identifiers.org/pubmed/1548743
xref: UnificationXref286
xref: http://identifiers.org/pubmed/15613341
xref: http://identifiers.org/pubmed/11527981
xref: UnificationXref285
left: myristoyl-CoA
left: GAG Polyprotein (P04591)
comment: The amino terminal glycine residue of HIV-1 Gag polyprotein is myristoylated (Henderson et al. 1992). Myristoylation of newly synthesized Gag occurs in the cytosol of the infected host cell, with myristoyl-CoA as the myristate donor and the host cell NMT2 enzyme as the catalyst. Human cells express two isoforms of N-myristoyl transferase (NMT) (Giang and Cravatt 1998). The argumant that the second isoform catalyzes this reaction is indirect, based on the the observations that a stable enzyme:substrate complex forms transiently during the reaction (Farazi et al. 2001), and that Gag polyprotein can be found complexed with NMT2 (but not NMT1) in HIV-1-infected human cells (Hill and Skowronski 2005).
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: Authored: Gillespie, ME, 2013-03-06
right: coenzyme A
right: N-myristoyl GAG (P04591) protein

Stoichiometry2
type: Stoichiometry
physical entity: Envelope glycoprotein gp160
stoichiometric coefficient: 3.0

Stoichiometry1
type: Stoichiometry
physical entity: Envelope glycoprotein gp160
stoichiometric coefficient: 3.0

Stoichiometry4
type: Stoichiometry
physical entity: Surface protein gp120 (P04578)
stoichiometric coefficient: 3.0

Stoichiometry3
type: Stoichiometry
physical entity: Glycosylated Envelope glycoprotein gp160
stoichiometric coefficient: 3.0

UnificationXref350
type: UnificationXref
id: 939199
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939199

UnificationXref351
type: UnificationXref
id: REACT_27339
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27339.2

UnificationXref354
type: UnificationXref
id: 939237
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939237

UnificationXref355
type: UnificationXref
id: REACT_27795
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27795.2

UnificationXref352
type: UnificationXref
id: 939221
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939221

UnificationXref353
type: UnificationXref
id: REACT_27837
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27837.2

SequenceSite100
type: SequenceSite
position status: EQUAL
sequence position: 609

SequenceSite101
type: SequenceSite
position status: EQUAL
sequence position: 684

SequenceSite102
type: SequenceSite
position status: EQUAL
sequence position: 1

http://identifiers.org/pubmed/1760846
type: PublicationXref
id: 1760846
author: Engelman, A
author: Craigie, R
author: Mizuuchi, K
db: Pubmed
title: HIV-1 DNA integration: mechanism of viral DNA cleavage and DNA strand transfer
source: Cell 67:1211-21
year: 1991

http://identifiers.org/pubmed/16683918
type: PublicationXref
id: 16683918
author: Resh, MD
author: Perlman, M
db: Pubmed
title: Identification of an intracellular trafficking and assembly pathway for HIV-1 gag
source: Traffic 7:731-45
year: 2006

O-phospho-L-serine
type: SequenceModificationVocabulary
xref: UnificationXref102

UnificationXref368
type: UnificationXref
id: REACT_27927
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27927.1

UnificationXref367
type: UnificationXref
id: 184333
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184333

UnificationXref369
type: UnificationXref
id: Q9UK41
db: UniProt

UnificationXref360
type: UnificationXref
id: Q99816
db: UniProt

UnificationXref361
type: UnificationXref
id: 2980549
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2980549

UnificationXref362
type: UnificationXref
id: REACT_160839
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_160839.2

UnificationXref363
type: UnificationXref
id: Q86XT2
db: UniProt

UnificationXref364
type: UnificationXref
id: 184289
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=184289

UnificationXref365
type: UnificationXref
id: REACT_27635
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_27635.1

Synthesis and organization of GAG, GAGPOL polyproteins
type: Pathway
data source: Reactome
pathway component: Synthesis of GAG polyprotein
pathway component: Membrane binding and targetting of GAG proteins
xref: UnificationXref421
xref: RelationshipXref3
xref: UnificationXref420
xref: PublicationXref38
pathway order: PathwayStep19
pathway order: PathwayStep14
display name: Synthesis And Processing Of GAG, GAGPOL Polyproteins
comment: Reviewed: Dubé, Mathieu, 2013-05-21
comment: Authored: Gillespie, ME, 2013-03-06
comment: Evidence suggests that the RNA molecules used for the synthesis of Gag and Gag-Pro-Pol are not the same molecules that are packaged into virions. Gag proteins do not appear to aggregate around and capture the RNA contained in the polyribosome from which they emerged, but rather bind to and ultimately encapsidate free transcripts elsewhere. During the replication of retroviruses, large numbers of Gag molecules must be generated to serve as precursors to the structural proteins of the virions. Retroviruses have developed a mechanism that permits expression of the Gag protein at high levels relative to the protein sequences encoded in the pro and pol genes, while retaining coregulated expression. This linkage results from the use of the same initiation codon in the same mRNA to express the gag, pro, and pol genes. Translation of this RNA leads occasionally to synthesis of a fusion protein that is usually called the Gag-Pol precursor but is now more appropriately called the Gag-Pro-Pol precursor
organism: Human immunodeficiency virus 1

UnificationXref366
type: UnificationXref
id: Q8NEZ2
db: UniProt

SPT5H
type: ProteinReference
name: SPT5
name: UniProt:O00267 SUPT5H
name: SUPT5H
xref: UnificationXref161
comment: FUNCTION Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.SUBUNIT Interacts with SUPT4H1 to form DSIF. DSIF interacts with the positive transcription elongation factor b complex (P-TEFb complex), which is composed of CDK9 and cyclin-T (CCNT1 or CCNT2). DSIF interacts with RNA polymerase II, and this interaction is reduced by phosphorylation of the C-terminal domain (CTD) of POLR2A by P-TEFb. DSIF also interacts with the NELF complex, which is composed of WHSC2/NELFA, COBRA1/NELFB, TH1L/NELFD and RDBP/NELFE, and this interaction occurs following prior binding of DSIF to RNA polymerase II. DSIF also interacts with PRMT1/HRMT1L2, HTATSF1/TATSF1, RNGTT/CAP1A, PRMT5/SKB1, SUPT6H, and can interact with PIN1. Component of a complex which is …
organism: Homo sapiens

Same Catalyst Activity
type: RelationshipTypeVocabulary

http://identifiers.org/pubmed/15314653
type: PublicationXref
id: 15314653
author: Mitchell, RS
author: Chen, H
author: Ecker, JR
author: Beitzel, BF
author: Bushman, FD
author: Shinn, P
author: Berry, CC
author: Schroder, AR
db: Pubmed
title: Retroviral DNA integration: ASLV, HIV, and MLV show distinct target site preferences
source: PLoS Biol 2:E234
year: 2004

HIV-1 template:capped HIV-1 transcript hybrid
type: PhysicalEntity
data source: Reactome
xref: UnificationXref157
xref: UnificationXref156
cellular location: nucleoplasm
comment: Reactome DB_ID: 167074

viral minus strand DNA with sticky 3' end
type: PhysicalEntity
data source: Reactome
xref: UnificationXref201
xref: UnificationXref202
cellular location: nucleoplasm
comment: Reactome DB_ID: 175079

Rev-multimer
type: PhysicalEntity
data source: Reactome
xref: UnificationXref190
xref: UnificationXref189
cellular location: nucleoplasm
comment: Reactome DB_ID: 165542

Host genomic DNA
type: PhysicalEntity
data source: Reactome
xref: UnificationXref238
xref: UnificationXref237
cellular location: nucleoplasm
comment: Reactome DB_ID: 175158

viral plus strand DNA with sticky 3' end
type: PhysicalEntity
data source: Reactome
xref: UnificationXref204
xref: UnificationXref203
cellular location: nucleoplasm
comment: Reactome DB_ID: 175429

UBC
type: ProteinReference
name: UniProt:P0CG48 UBC
xref: UnificationXref300
comment: FUNCTION Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.MISCELLANEOUS Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.MISCELLANEOUS For the sake of clarity sequence features are annotated only for the first chain, and are not repeated for each of the following chains.SIMILARITY Belongs to the ubiquitin family.SIMILARITY Contains 9 ubiquitin-like domains.
organism: Homo sapiens

UniProt:P0CG47 UBB
type: ProteinReference
name: UBB
xref: UnificationXref293
comment: FUNCTION Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.MISCELLANEOUS Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.MISCELLANEOUS The mRNA encoding variant UBB(+1) is produced by an unknown mechanism involving the deletion of a GT dinucleotide in the close proximity of a GAGAG motif (PubMed:9422699). This variant mRNA is found in normal brain, but the encoded protein accumulates only in brain …
organism: Homo sapiens

UniProt:Q99816 TSG101
type: ProteinReference
name: TSG101
xref: UnificationXref360
comment: FUNCTION Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.SUBUNIT Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoechiometry. Interacts with VPS37A, VPS37B and VPS37C. Interacts with ubiquitin, stathmin, GMCL, DMAP1 and AATF (By similarity). Interacts with HGS; the interaction mediates the association with the ESCRT-0 complex. Interacts with GGA1 and GGA3. Interacts (via UEV domain) with PDCD6IP/AIP1. Interacts with VPS28, SNF8 and VPS36. Self-associates. Interacts with MVB12A; the association appears to be mediated by the TSG101-VPS37 binary subcomplex. Interacts with VPS37D. Interacts with LRSAM1. Interacts with CEP55; the interaction is required for cytokinesis but not for viral budding. Interacts with PDCD6. Interacts with HIV-1 p6. Interacts with human spumavirus Gag. Interacts with HTLV-1 Gag. Interacts with Ebola virus VP40. Interacts with EIAV p9; the interaction has been shown in vitro. Interacts with MGRN1.TISSUE SPECIFICITY Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.DOMAIN The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.DOMAIN The coiled coil domain may interact…
organism: Homo sapiens

TH1
type: ProteinReference
name: HSPC130
name: TH1L
name: NELFCD
name: NELFD
name: UniProt:Q8IXH7 NELFCD
xref: UnificationXref141
comment: FUNCTION Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.SUBUNIT The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE. Interacts with ARAF1.TISSUE SPECIFICITY Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal and cardiac muscle, adrenal, thyroid, kidney and pancreas.SIMILARITY Belongs to the NELF-D family.
organism: Homo sapiens

UniProt:P30876 POLR2B
type: ProteinReference
name: POLR2B
xref: UnificationXref111
comment: CATALYTIC ACTIVITY Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SUBUNIT Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. Interacts with WDR82. Interacts with MEN1.SIMILARITY Belongs to the RNA polymerase beta chain family.
organism: Homo sapiens

http://identifiers.org/pubmed/10620603
type: PublicationXref
id: 10620603
author: Susin, SA
author: Druillennec, S
author: Vieira, HL
author: Hoebeke, J
author: Briand, JP
author: Reed, JC
author: Roques, BP
author: Loeffler, M
author: Costantini, P
author: Xie, ZH
author: Jacotot, E
author: Zamzami, N
author: Ravagnan, L
author: Daugas, E
author: Kroemer, G
author: Cointe, D
author: Ferri, KF
author: Irinopoulou, T
db: Pubmed
title: The HIV-1 viral protein R induces apoptosis via a direct effect on the mitochondrial permeability transition pore
source: J Exp Med 191:33-46
year: 2000

UniProt:P36954 POLR2I
type: ProteinReference
name: POLR2I
xref: UnificationXref114
comment: SUBUNIT Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.SIMILARITY Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.SIMILARITY Contains 1 TFIIS-type zinc finger.
organism: Homo sapiens

viral minus strand DNA (ful-length)
type: PhysicalEntity
data source: Reactome
xref: UnificationXref255
xref: UnificationXref256
cellular location: nucleoplasm
comment: Reactome DB_ID: 175411

genomic DNA with staggered 5' ends
type: PhysicalEntity
data source: Reactome
xref: UnificationXref241
xref: UnificationXref242
cellular location: nucleoplasm
comment: Reactome DB_ID: 175520

viral plus strand DNA (full-length)
type: PhysicalEntity
data source: Reactome
xref: UnificationXref254
xref: UnificationXref253
cellular location: nucleoplasm
comment: Reactome DB_ID: 175036

UniProt:P69726 vpr
type: ProteinReference
name: vpr
xref: UnificationXref3
comment: MISCELLANEOUS HIV-1 lineages are divided in three main groups, M (for Major), O (for Outlier), and N (for New, or Non-M, Non-O). The vast majority of strains found worldwide belong to the group M. Group O seems to be endemic to and largely confined to Cameroon and neighboring countries in West Central Africa, where these viruses represent a small minority of HIV-1 strains. The group N is represented by a limited number of isolates from Cameroonian persons. The group M is further subdivided in 9 clades or subtypes (A to D, F to H, J and K).SIMILARITY Belongs to the HIV-1 VPR protein family.
organism: Human immunodeficiency virus 1

vif
type: ProteinReference
name: UniProt:P69723 vif
xref: UnificationXref217
comment: FUNCTION Counteracts the innate antiviral activity of human APOBEC3F and APOBEC3G. Forms a complex with host APOBEC3F and APOBEC3G thus preventing the entry of these lethally hypermutating enzymes into progeny virions. Recruits an active E3 ubiquitin ligase complex composed of elongin BC, CUL5, and RBX2 to induce polyubiquitination of APOBEC3G and APOBEC3F. In turn, they are directed to the 26S proteasome for degradation. Vif interaction with APOBEC3G also blocks its cytidine deaminase activity in a proteasome-independent manner, suggesting a dual inhibitory mechanism. May interact directly with APOBEC3G mRNA in order to inhibit its translation. Seems to play a role in viral morphology by affecting the stability of the viral nucleoprotein core. Finally, Vif also contributes to the G2 cell cycle arrest observed in HIV infected cells.SUBUNIT Homomultimer; in vitro and presumably in vivo. Interacts with viral RNA and Pr55Gag precursor; these interactions mediate Vif incorporation into the virion. Interacts with the viral reverse transcriptase. Interacts with human APOBEC3F and APOBEC3G. Interacts with host UBCE7IP1 isoform 3/ZIN and possibly with SAT. Interacts with host tyrosine kinases HCK and FYN; these interactions may decrease level of phosphorylated APOBEC3G incorporation into virions. Interacts with host ABCE1; this interaction may play a role in protecting viral RNA from damage during viral assembly. Forms an E3 ligase complex by interacting with human CUL5 and elongin BC complex (TCEB1 and TCEB2). Interacts with host MDM2; this interaction targets Vif for degradation by the proteasome.INDUCTION Expressed late during infection in a Rev-dependent manner.PTM Highly phosphorylated on serines and threonines residues. Thr-96 and Ser-165 are phosphorylated by the mitogen activated kinase MAP4K1. As the HIV-1 replication can be activated by stress and mitogens, these phosphorylations could be involved in this process. Ser-144 phosphorylation may inhibit elongin BC com…
organism: Human immunodeficiency virus 1

PublicationXref38
type: PublicationXref
id: 0-87969-571-4
author: Wills, JW
author: Swanstrom, R
db: ISBN
title: Synthesis, Assembly, and Processing of Viral Proteins
source: Retroviruses (Book)
year: 1997

http://identifiers.org/pubmed/15473846
type: PublicationXref
id: 15473846
author: Morita, E
author: Sundquist, WI
db: Pubmed
title: Retrovirus budding
source: Annu Rev Cell Dev Biol 20:395-425
year: 2004

Myristoyl-CoA
type: SmallMoleculeReference
name: Tetradecanoyl-CoA [ChEBI:15532]
name: Tetradecanoyl-CoA
xref: UnificationXref273

ESCRT-I
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry97
component stoichiometry: Stoichiometry94
component stoichiometry: Stoichiometry92
component stoichiometry: Stoichiometry95
component stoichiometry: Stoichiometry93
component stoichiometry: Stoichiometry96
component: VPS37A
component: VPS28
component: Tumor susceptibility gene 101 protein
component: VPS37B
component: VPS37D
component: VPS37C
xref: UnificationXref379
xref: UnificationXref378
cellular location: endosome membrane
comment: Reactome DB_ID: 184398

monoubiquitinated N-myristoyl GAG (P04591) protein
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry99
component stoichiometry: Stoichiometry98
component: N-myristoyl GAG
component: ubiquitin
xref: UnificationXref415
xref: UnificationXref414
cellular location: plasma membrane
comment: Reactome DB_ID: 3149449

EntityFeature1
type: EntityFeature
feature location: SequenceInterval10
comment: Phosphorylated (Ser5) CTD of RNA pol II largest subunit

http://identifiers.org/pubmed/9506952
type: PublicationXref
id: 9506952
author: Cravatt, BF
author: Giang, DK
db: Pubmed
title: A second mammalian N-myristoyltransferase
source: J Biol Chem 273:6595-8
year: 1998

gene ontology term for cellular function
type: RelationshipTypeVocabulary
xref: UnificationXref51

UnificationXref300
type: UnificationXref
id: P0CG48
db: UniProt

PublicationXref28
type: PublicationXref
author: Bushman, FD
title: Retroviral cDNA integration: mechanism, applications and inhibition.(Ed) Setlow JK
source: Genetic Engineering Principles and Methods 20:41-62
year: 1998

gene ontology term for cellular process
type: RelationshipTypeVocabulary
xref: UnificationXref74

Golgi membrane
type: CellularLocationVocabulary
xref: UnificationXref30

IN bound to sticky 3' ends of viral DNA
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry58
component stoichiometry: Stoichiometry55
component: viral DNA with 3' sticky ends
component: IN (Integrase) (P04585) protein
xref: UnificationXref208
xref: UnificationXref207
cellular location: nucleoplasm
comment: Reactome DB_ID: 175332

viral DNA with 3' sticky ends
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry57
component stoichiometry: Stoichiometry56
component: viral plus strand DNA with sticky 3' end
component: viral minus strand DNA with sticky 3' end
xref: UnificationXref206
xref: UnificationXref205
cellular location: nucleoplasm
comment: Reactome DB_ID: 175146

Integration intermediate
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry81
component stoichiometry: Stoichiometry82
component: genomic DNA with staggered 5' ends
component: IN:viral DNA bound to host genomic DNA
xref: UnificationXref250
xref: UnificationXref249
cellular location: nucleoplasm
comment: Reactome DB_ID: 175148

IN:viral DNA bound to host genomic DNA
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry79
component stoichiometry: Stoichiometry80
component: Host genomic DNA
component: IN bound to sticky 3' ends of viral DNA
xref: UnificationXref247
xref: UnificationXref248
cellular location: nucleoplasm
comment: Reactome DB_ID: 175339

viral PIC proteins
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry74
component stoichiometry: Stoichiometry73
component stoichiometry: Stoichiometry69
component stoichiometry: Stoichiometry75
component stoichiometry: Stoichiometry70
component stoichiometry: Stoichiometry71
component stoichiometry: Stoichiometry72
component stoichiometry: Stoichiometry68
component stoichiometry: Stoichiometry76
component: Matrix
component: REV (P04618) protein
component: BAF protein
component: p6 protein
component: VIF (P69723) protein
component: VPU (P05919) protein
component: VPR (P69726) protein
component: HMGA1_HUMAN
component: PC4 and SFRS1-interacting protein
xref: UnificationXref239
xref: UnificationXref240
cellular location: nucleoplasm
comment: Reactome DB_ID: 177527

UnificationXref309
type: UnificationXref
id: 939232
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939232

IN:viral DNA bound to host genomic DNA with staggered ends
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry77
component stoichiometry: Stoichiometry78
component: genomic DNA with staggered 5' ends
component: IN bound to sticky 3' ends of viral DNA
xref: UnificationXref243
xref: UnificationXref244
cellular location: nucleoplasm
comment: Reactome DB_ID: 175224

monoubiquitinated N-myristoyl GAG (P04591) protein
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry88
component stoichiometry: Stoichiometry89
component: ubiquitin
component: N-myristoyl GAG (P04591) protein
xref: UnificationXref322
xref: UnificationXref321
cellular location: cytosol
comment: Reactome DB_ID: 184369

UnificationXref308
type: UnificationXref
id: REACT_24880
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24880.2

monoubiquitinated N-myristoyl GAG (P04591) protein
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry90
component stoichiometry: Stoichiometry91
component: ubiquitin
component: N-myristoyl GAG (P04591) protein
xref: UnificationXref359
xref: UnificationXref358
cellular location: endosome membrane
comment: Reactome DB_ID: 184475

UnificationXref307
type: UnificationXref
id: 939192
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939192

UnificationXref306
type: UnificationXref
id: REACT_24765
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24765.2

Integrated provirus
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry87
component stoichiometry: Stoichiometry86
component: linear duplex viral DNA
component: Host genomic DNA
xref: UnificationXref260
xref: UnificationXref259
cellular location: nucleoplasm
display name: provirus
comment: Reactome DB_ID: 175486

UnificationXref305
type: UnificationXref
id: 939258
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939258

linear duplex viral DNA
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry85
component stoichiometry: Stoichiometry84
component: viral minus strand DNA (ful-length)
component: viral plus strand DNA (full-length)
xref: UnificationXref257
xref: UnificationXref258
cellular location: nucleoplasm
comment: Reactome DB_ID: 175124

UnificationXref304
type: UnificationXref
id: REACT_24312
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24312.2

UnificationXref303
type: UnificationXref
id: 939164
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939164

UnificationXref302
type: UnificationXref
id: REACT_24844
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24844.2

UnificationXref301
type: UnificationXref
id: 939188
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=939188

PublicationXref18
type: PublicationXref
author: Varmus, HE
author: Hughes, SH
author: Coffin, JM
title: Integration
source: Retroviruses (Book) 5:161-204
year: 1997

RNA Polymerase II holoenzyme complex (hyperphosphorylated)
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry16
component stoichiometry: Stoichiometry23
component stoichiometry: Stoichiometry17
component stoichiometry: Stoichiometry27
component stoichiometry: Stoichiometry25
component stoichiometry: Stoichiometry20
component stoichiometry: Stoichiometry22
component stoichiometry: Stoichiometry19
component stoichiometry: Stoichiometry21
component stoichiometry: Stoichiometry24
component stoichiometry: Stoichiometry18
component stoichiometry: Stoichiometry26
component: POLR2G
component: RNA polymerase II subunit 3
component: DNA-directed RNA polymerase II 23 kDa polypeptide (EC 2.7.7.6) (RPB25) (XAP4) (RPB5)
component: DNA-directed RNA polymerase II 14.4 kDa polypeptide (EC 2.7.7.6) (RPB6) (RPB14.4)
component: RPABC4
component: RPB14.5
component: DNA-directed RNA polymerase II 16 kDa polypeptide
component: DNA-directed RNA polymerase II 13.3 kDa polypeptide
component: DNA-directed RNA polymerase II 140 kDa polypeptide
component: DNA-directed RNA polymerase II 7.6 kDa polypeptide
component: POLR2H
component: p-S2,S5-POLR2A
xref: UnificationXref130
xref: UnificationXref129
cellular location: nucleoplasm
comment: Reactome DB_ID: 109909

TFIIF
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry29
component stoichiometry: Stoichiometry30
component: Transcription initiation factor RAP30
component: Transcription initiation factor IIF, alpha subunit
xref: UnificationXref137
xref: UnificationXref138
cellular location: nucleoplasm
comment: Reactome DB_ID: 109631

phospho-NELF complex
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry33
component stoichiometry: Stoichiometry34
component stoichiometry: Stoichiometry36
component stoichiometry: Stoichiometry35
component: Negative elongation factor B
component: Negative elongation factor A
component: phospho-RD
component: HSPC130
xref: UnificationXref154
xref: UnificationXref155
cellular location: nucleoplasm
comment: Reactome DB_ID: 170709

DSIF complex
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry39
component stoichiometry: Stoichiometry40
component: SPT4H1 protein
component: Transcription elongation factor SPT5
xref: UnificationXref165
xref: UnificationXref164
cellular location: nucleoplasm
comment: Reactome DB_ID: 112420

RNA Polymerase II holoenzyme complex (hyperphosphorylated):TFIIF complex
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry31
component stoichiometry: Stoichiometry28
component: TFIIF
component: RNA Polymerase II holoenzyme complex (hyperphosphorylated)
xref: UnificationXref139
xref: UnificationXref140
cellular location: nucleoplasm
comment: Reactome DB_ID: 113425

IN bound to sticky 3' ends of viral DNA in PIC
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry59
component stoichiometry: Stoichiometry63
component stoichiometry: Stoichiometry66
component stoichiometry: Stoichiometry61
component stoichiometry: Stoichiometry60
component stoichiometry: Stoichiometry64
component stoichiometry: Stoichiometry65
component stoichiometry: Stoichiometry62
component stoichiometry: Stoichiometry67
component stoichiometry: Stoichiometry54
component: Matrix
component: REV (P04618) protein
component: BAF protein
component: IN bound to sticky 3' ends of viral DNA
component: p6 protein
component: VIF (P69723) protein
component: VPU (P05919) protein
component: VPR (P69726) protein
component: HMGA1_HUMAN
component: PC4 and SFRS1-interacting protein
xref: UnificationXref235
xref: UnificationXref236
cellular location: nucleoplasm
comment: Reactome DB_ID: 175416

Tat-containing early elongation complex with hyperphosphorylated Pol II CTD ( phospho-NELF phospho DSIF)
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry48
component stoichiometry: Stoichiometry46
component stoichiometry: Stoichiometry44
component stoichiometry: Stoichiometry49
component stoichiometry: Stoichiometry47
component stoichiometry: Stoichiometry45
component: Tat:P-TEFb(Cyclin T1:Cdk9) complex
component: phospho-NELF complex
component: FCP1P protein
component: RNA Polymerase II holoenzyme complex (hyperphosphorylated):TFIIF complex
component: phospho-DSIF complex
component: HIV-1 template:capped HIV-1 transcript hybrid
xref: UnificationXref176
xref: UnificationXref177
cellular location: nucleoplasm
comment: Reactome DB_ID: 170710

phospho-DSIF complex
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry43
component stoichiometry: Stoichiometry42
component: SPT4H1 protein
component: Transcription elongation factor SPT5
xref: UnificationXref174
xref: UnificationXref175
cellular location: nucleoplasm
comment: Reactome DB_ID: 170705

Rev-bound HIV-1 mRNA
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry50
component stoichiometry: Stoichiometry51
component: REV (P04618) protein
component: HIV-1 mRNA
xref: UnificationXref188
xref: UnificationXref187
cellular location: nucleoplasm
comment: Reactome DB_ID: 165535

Rev multimer-bound HIV-1 mRNA
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry52
component stoichiometry: Stoichiometry53
component: Rev-bound HIV-1 mRNA
component: Rev-multimer
xref: UnificationXref191
xref: UnificationXref192
cellular location: nucleoplasm
comment: Reactome DB_ID: 165532