http://identifiers.org/pubmed/5445796
type: PublicationXref
id: 5445796
author: Greengard, P
author: Kuo, JF
db: Pubmed
title: Cyclic nucleotide-dependent protein kinases. VI. Isolation and partial purification of a protein kinase activated by guanosine 3',5'-monophosphate
source: J Biol Chem 245:2493-8
year: 1970

UnificationXref10
type: UnificationXref
id: REACT_23977
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_23977.1

Activated cGMP-dependent protein kinase (PKG)
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry3
component stoichiometry: Stoichiometry2
component: cGMP-dependent protein kinase
component: Cyclic GMP
xref: UnificationXref7
xref: UnificationXref8
cellular location: cytosol
comment: Reactome DB_ID: 427305

UnificationXref3
type: UnificationXref
id: REACT_24826
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24826.2

UnificationXref4
type: UnificationXref
id: CHEBI:16356
db: ChEBI

Stoichiometry3
type: Stoichiometry
physical entity: Cyclic GMP
stoichiometric coefficient: 1.0

UnificationXref1
type: UnificationXref
id: GO:0005829
db: GENE ONTOLOGY

Stoichiometry2
type: Stoichiometry
physical entity: cGMP-dependent protein kinase
stoichiometric coefficient: 1.0

UnificationXref2
type: UnificationXref
id: 427304
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=427304

Cyclic GMP
type: SmallMoleculeReference
name: Guanosine 3',5'-cyclic phosphate
name: Guanosine 3',5'-cyclic monophosphate
name: 3',5'-Cyclic GMP
name: 3',5'-Cyclic GMP [ChEBI:16356]
xref: UnificationXref4

Reactome
type: Provenance
comment: http://www.reactome.org

cGMP-dependent protein kinase
type: PhysicalEntity
data source: Reactome
xref: UnificationXref2
xref: UnificationXref3
cellular location: cytosol
display name: PKG
comment: Reactome DB_ID: 427304

cytosol
type: CellularLocationVocabulary
xref: UnificationXref1

Cyclic GMP
type: SmallMolecule
entity reference: Cyclic GMP
data source: Reactome
name: Guanosine 3',5'-cyclic phosphate
name: Guanosine 3',5'-cyclic monophosphate
name: 3',5'-Cyclic GMP
xref: UnificationXref6
xref: UnificationXref5
cellular location: cytosol
comment: Reactome DB_ID: 30991
display name: cGMP

http://identifiers.org/pubmed/15545263
type: PublicationXref
id: 15545263
author: Hofmann, F
db: Pubmed
title: The biology of cyclic GMP-dependent protein kinases
source: J Biol Chem 280:1-4
year: 2005

UnificationXref8
type: UnificationXref
id: REACT_24100
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24100.1

UnificationXref7
type: UnificationXref
id: 427305
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=427305

cGMP stimulates Protein Kinase G
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/15545263
xref: http://identifiers.org/pubmed/10209042
xref: UnificationXref10
xref: UnificationXref9
xref: http://identifiers.org/pubmed/5445796
participant stoichiometry: Stoichiometry1
left: cGMP-dependent protein kinase
left: Cyclic GMP
comment: Reviewed: Kunapuli, SP, 2010-06-07
comment: Authored: Akkerman, JW, 2009-06-03
comment: Edited: Jupe, S, 2010-06-07
comment: Protein Kinase G (PKG) is a homodimer held together by a leucine zipper present in the N terminus. Each member of the dimer has two cyclic GMP (cGMP) binding sites, one low affinity and one high affinity. PKG was first described in various arthropods. Mammals have two PKG genes, prkg1 and prkg2, that encode PKG1 (cGKI) and PKG2 (cGKII). The N terminus (the first 90-100 residues) of PKG1 is encoded by two alternatively spliced exons that produce the isoforms PKG1alpha and PKG1beta. Both are cytosolic. PKG1 is present in high concentrations (>0.1 µM) in all smooth muscles, platelets, cerebellum, hippocampus, dorsal root ganglia, neuromuscular endplate, and kidney. PKG1beta is the predominant PKG isoform in platelets. PKG1 is required for the inhibition of platelet activation by NO/cGMP. PKG2 is anchored at the plasma membrane by myristoylation of the N-terminal Gly-2 residue. PKG2 phosphorylates cystic fibrosis transmembrane conductance regulator.
right: Activated cGMP-dependent protein kinase (PKG)

Stoichiometry1
type: Stoichiometry
physical entity: Cyclic GMP
stoichiometric coefficient: 2.0

UnificationXref6
type: UnificationXref
id: REACT_24220
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_24220.2

UnificationXref5
type: UnificationXref
id: 30991
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=30991

http://identifiers.org/pubmed/10209042
type: PublicationXref
id: 10209042
author: Siess, W
author: Ruth, P
author: Fässler, R
author: Klatt, P
author: Hofmann, F
author: Massberg, S
author: Sausbier, M
author: Pfeifer, A
author: Bauer, M
author: Krombach, F
db: Pubmed
title: Increased adhesion and aggregation of platelets lacking cyclic guanosine 3',5'-monophosphate kinase I
source: J Exp Med 189:1255-64
year: 1999

UnificationXref9
type: UnificationXref
id: 427308
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=427308