Mus musculus
type: BioSource
xref: UnificationXref125

GDP-L-fucose
type: SmallMoleculeReference
name: GDP-L-fucose [ChEBI:5221]
xref: UnificationXref145

UniProt:P97260 SCAP
type: ProteinReference
name: SCAP
xref: UnificationXref3
comment: FUNCTION Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway.DOMAIN Cholesterol bound to SSC domain of SCAP or oxysterol bound to INSIG1/2 leads to masking of an ER export signal on SCAP possibly by moving the signal further away from the ER membrane.DISRUPTION PHENOTYPE CHO cells show loss of site-1 cleavage of SREBF1/2, reduced synthesis of cholesterol, a reduced level of LDLR and cholesterol auxotrophy.SIMILARITY Belongs to the WD repeat SCAP family.SIMILARITY Contains 1 SSD (sterol-sensing) domain.SIMILARITY Contains 7 WD repeats.
organism: Cricetulus griseus

SequenceSite5
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite4
type: SequenceSite
position status: EQUAL
sequence position: 1141

SequenceSite7
type: SequenceSite
position status: EQUAL
sequence position: 31

SequenceSite6
type: SequenceSite
position status: EQUAL
sequence position: 225

SequenceSite1
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite3
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite2
type: SequenceSite
position status: EQUAL
sequence position: 1276

INSIG1
type: ProteinReference
name: UniProt:O15503 INSIG1
xref: UnificationXref101
comment: FUNCTION Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.SUBUNIT Binds to the SCAP-SREBF2 complex only in the presence of sterols. Interacts with RNF139. Interacts with HMGCR (via its SSD); the interaction, accelerated by sterols, leads to the recruitment of HMGCR to AMFR/gp78 for its ubiquitination by the sterol-mediated ERAD pathway. Interacts with AMFR/gp78 (via its membrane domain); the interaction recruits HMCR at the ER membrane for its ubiquitination and degradation by the sterol-mediated ERAD pathway.TISSUE SPECIFICITY Expressed in all tissues tested with highest expression in the liver.INDUCTION By insulin.PTM Ubiquitinated. Subsequent to sterol deprivation, the SCAP-SREBF2 complex becomes dissociated from INSIG1, is then ubiquitinated and degraded in proteasomes. Although ubiquitination is required for rapid INSIG1 degradation, it is not required for release of the SCAP-SREBP complex. Ubiquitinated by RNF139.MISCELLANEOUS Expressed at high levels when nuclear SREBP levels are high as a result of sterol deprivation.SIMILARITY Belongs to the INSIG family.
organism: Homo sapiens

SequenceSite8
type: SequenceSite
position status: EQUAL
sequence position: 74

SequenceSite9
type: SequenceSite
position status: EQUAL
sequence position: 190

SequenceSite12
type: SequenceSite
position status: EQUAL
sequence position: 424

SequenceSite11
type: SequenceSite
position status: EQUAL
sequence position: 307

SequenceSite14
type: SequenceSite
position status: EQUAL
sequence position: 650

SequenceSite13
type: SequenceSite
position status: EQUAL
sequence position: 575

SequenceSite16
type: SequenceSite
position status: EQUAL
sequence position: 763

SequenceSite15
type: SequenceSite
position status: EQUAL
sequence position: 725

SequenceSite18
type: SequenceSite
position status: EQUAL
sequence position: 879

SequenceSite17
type: SequenceSite
position status: EQUAL
sequence position: 841

SequenceSite10
type: SequenceSite
position status: EQUAL
sequence position: 269

Homo sapiens
type: BioSource
xref: UnificationXref8

Cricetulus griseus
type: BioSource
xref: UnificationXref2

UnificationXref10
type: UnificationXref
id: 1655733
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655733

UDP-D-galactose [ChEBI:18307]
type: SmallMoleculeReference
name: UDP-D-galactopyranose
name: UDPgalactose
name: UDP-D-galactose
xref: UnificationXref45

UnificationXref17
type: UnificationXref
id: Q9Y5U4
db: UniProt

Fucosylation of Pre-Notch by POFUT1
type: BiochemicalReaction
data source: Reactome
EC number: 2.4.1.221
xref: http://identifiers.org/pubmed/12486116
xref: UnificationXref179
xref: http://identifiers.org/pubmed/21464368
xref: http://identifiers.org/pubmed/11524432
xref: UnificationXref178
xref: http://identifiers.org/pubmed/18347015
xref: http://identifiers.org/pubmed/15692013
participant stoichiometry: Stoichiometry28
participant stoichiometry: Stoichiometry29
left: Noth precursors
left: GDP-L-fucose
comment: Edited: Orlic-Milacic, M, 2012-02-10
comment: Authored: Egan, SE, Orlic-Milacic, M, 2011-11-14
comment: Reviewed: Haw, R, 2012-02-06
comment: Edited: D'Eustachio, P, 2012-02-06
comment: In the endoplasmic reticulum, Notch receptor precursors are fucosylated on conserved serine and threonine residues in their EGF repeats. The consensus fucosylation site sequence is C2-X(4-5)-S/T-C3, where C2 and C3 are the second and third cysteine residue within the EGF repeat, and X(4-5) is four to five amino acid residues of any type. Only those fucosylation sites that are conserved between human, mouse and rat Notch isoforms are annotated. Fucosylation is performed by the endoplasmic reticulum resident O-fucosyl transferase POFUT1. Fucosylation by POFUT1, initially discovered in Drosophila, is considered to be an essential step in Notch processing and production of a fully functional receptor. Fucosylation of mammalian Notch proteins was studied by exogenously expressing mouse Notch1 in Chinese hamster ovary cell lines.
right: Guanosine 5'-Diphosphate
right: Fucosylated Notch precursors

UnificationXref18
type: UnificationXref
id: 1655736
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655736

SREBF2:SCAP Transits to the Golgi
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/12842885
xref: UnificationXref142
xref: UnificationXref143
left: SREBP2:SCAP:CopII Coat
display name: SREBP2:SCAP Transits to the Golgi
comment: Authored: May, B, 2011-09-28
comment: Edited: May, B, 2011-09-28
comment: In low concentrations of cholesterol SCAP interacts with Sec24 of the CopII coat complex causing SCAP:SREBP1A/1C/2 to be transported with the CopII complex from the endoplasmic reticulum to the Golgi.
right: SCAP:SREBF2

UnificationXref15
type: UnificationXref
id: 2317518
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2317518

SREBP1A/1C/2 is retained in the endoplasmic reticulum by SCAP:cholesterol:INSIG
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/12842885
xref: http://identifiers.org/pubmed/9242699
xref: UnificationXref121
xref: http://identifiers.org/pubmed/12202038
xref: http://identifiers.org/pubmed/15452130
xref: http://identifiers.org/pubmed/15728349
xref: UnificationXref120
xref: http://identifiers.org/pubmed/12963821
xref: http://identifiers.org/pubmed/15899885
participant stoichiometry: Stoichiometry11
participant stoichiometry: Stoichiometry12
left: SREBF1A/1C/2:SCAP
left: INSIG
left: Cholesterol
comment: Authored: May, B, 2012-06-10
comment: Edited: May, B, 2012-06-10
comment: SREBPs (SREBP1A/1C/2, SREBFs) bind SCAP in the endoplasmic reticulum membrane. Luminal loop 1 of SCAP binds cholesterol (Adams et al. 2003) which prevents SCAP from interacting with Sec24 in the CopII coat complex and allows SCAP to interact with INSIG instead (Sakai et al. 1997, Yang et al. 2002, Dobrosotskaya et al. 2003, Adams et al. 2004, Feramisco et al. 2005, Sun et al. 2005). These interactions retain SCAP:SREBP1A/1C/2 in the endoplasmic reticulum.
right: SREBP1A/1C/2:SCAP:cholesterol:INSIG

Sialylation of Pre-Notch
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/11530204
xref: UnificationXref96
xref: http://identifiers.org/pubmed/10935626
xref: UnificationXref97
left: CMP-sialic acid
left: Glc,Gal-GlcNAc-Fuc-Pre-Notch
comment: Edited: Orlic-Milacic, M, 2012-02-10
comment: Authored: Egan, SE, Orlic-Milacic, M, 2011-11-14
comment: Using Chinese hamster (Cricetulus griseus) ovary cells that express endogenous Notch1 but do not express endogenous Fringe proteins, Moloney et al. found after introduction of mouse Mfng that mature Notch1 had the Sia-alpha2,3-Gal-beta1,4-GlcNAc-beta1,3-fucitol tetrasaccharide attached to conserved serine and threonine residues in EGF repeats of Notch1 (Moloney et al. 2000). Sialyltransferases that could be involved in formation of this type of tetrasaccharide are St3gal3, St3gal4 and St3gal6 (Harduin-Lepers et al. 2001).
comment: Reviewed: Haw, R, 2012-02-06
comment: Edited: D'Eustachio, P, 2012-02-06
right: Glc,Sia-Gal-GlcNAc-Fuc-Pre-Notch
right: Cytidine-5'-monophosphate

UnificationXref16
type: UnificationXref
id: REACT_148138
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148138.1

UnificationXref13
type: UnificationXref
id: REACT_148265
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148265.1

SequenceSite187
type: SequenceSite
position status: EQUAL
sequence position: 961

UnificationXref14
type: UnificationXref
id: CHEBI:53030
db: ChEBI

SequenceSite188
type: SequenceSite
position status: EQUAL
sequence position: 999

UnificationXref11
type: UnificationXref
id: REACT_148522
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148522.2

SequenceSite189
type: SequenceSite
position status: EQUAL
sequence position: 1037

SREBF2:SCAP Binds CopII Coat Complex
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/12842885
xref: http://identifiers.org/pubmed/9242699
xref: http://identifiers.org/pubmed/12193656
xref: http://identifiers.org/pubmed/19041766
xref: http://identifiers.org/pubmed/15260976
xref: http://identifiers.org/pubmed/15452130
xref: UnificationXref180
xref: UnificationXref181
xref: http://identifiers.org/pubmed/21454655
xref: http://identifiers.org/pubmed/12202038
xref: http://identifiers.org/pubmed/15728349
xref: http://identifiers.org/pubmed/17428919
xref: http://identifiers.org/pubmed/12242332
xref: http://identifiers.org/pubmed/15899885
xref: http://identifiers.org/pubmed/12963821
participant stoichiometry: Stoichiometry32
participant stoichiometry: Stoichiometry31
participant stoichiometry: Stoichiometry30
left: SCAP:SREBF2
left: SC23A_MOUSE
left: S24C_HUMAN
left: SAR1B_CRIGR
display name: SREBP2:SCAP Binds CopII Coat Complex
comment: Authored: May, B, 2011-09-28
comment: Edited: May, B, 2011-09-28
comment: SREBP2 (SREBF2 and by inference SREBP1A and SREBP1C) is a transmembrane protein that binds SCAP in the endoplasmic reticulum membrane (Sakai et al. 1997). In the presence of cholesterol or oxysterols SCAP:SREBP2 binds INSIG and is retained in the endoplasmic reticulum (Espenshade et al. 2002, Yabe et al. 2002, Yang et al. 2002, Adams et al. 2003, Dobrosotskaya et al. 2003, Adams et al. 2004, Radhakrishnan et al. 2004, Sun et al. 2005, Sun et al. 2007, Motamed et al. 2011). At cholesterol concentrations below 5 mol% SCAP changes conformation, SCAP:SREBP2 loses interaction with INSIG, binds the CopII coat complex, and is translocated to the Golgi (Adams et al 2004, Feramisco et al. 2005, Radhakrishnan et al. 2008, Motamed et al. 2011).
right: SREBP2:SCAP:CopII Coat

UnificationXref12
type: UnificationXref
id: 2317524
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2317524

SequenceSite183
type: SequenceSite
position status: EQUAL
sequence position: 694

SequenceSite184
type: SequenceSite
position status: EQUAL
sequence position: 769

SequenceSite185
type: SequenceSite
position status: EQUAL
sequence position: 807

SequenceSite186
type: SequenceSite
position status: EQUAL
sequence position: 923

SequenceSite180
type: SequenceSite
position status: EQUAL
sequence position: 312

UnificationXref19
type: UnificationXref
id: REACT_148247
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148247.2

SequenceSite181
type: SequenceSite
position status: EQUAL
sequence position: 350

SequenceSite182
type: SequenceSite
position status: EQUAL
sequence position: 468

UnificationXref21
type: UnificationXref
id: REACT_148058
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148058.1

UnificationXref20
type: UnificationXref
id: 2317521
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2317521

SREBP2 (SREBF2) is retained in the endoplasmic reticulum by SCAP:INSIG2:oxysterol
type: BiochemicalReaction
data source: Reactome
xref: http://identifiers.org/pubmed/15452130
xref: http://identifiers.org/pubmed/17428920
xref: UnificationXref25
xref: UnificationXref24
participant stoichiometry: Stoichiometry6
left: SCAP:SREBF2
left: INSIG2:oxysterol
comment: Authored: May, B, 2012-06-10
comment: Edited: May, B, 2012-06-10
comment: INSIG binds oxysterols and the INSIG:oxysterol complex interacts with SCAP subunits of the SREBP2:SCAP (SREBF2:SCAP) complex (Adams et al. 2004, Radhakrishnan et al. 2007). This interaction retains the SREBP2:SCAP:INSIG:oxysterol complex in the endoplasmic reticulum. From this reaction it is inferred that SREBP1A (SREBF1A) and SREBP1C (SREBF1C) are retained similarly.
right: SREBF2:SCAP:INSIG2:oxysterol

Galactosylation of Pre-Notch
type: BiochemicalReaction
data source: Reactome
EC number: 2.4.1.38
xref: UnificationXref68
xref: http://identifiers.org/pubmed/11707585
xref: UnificationXref67
left: Glc,GlcNAc-Fuc-Pre-Notch
left: UDP-D-galactopyranose
comment: Edited: Orlic-Milacic, M, 2012-02-10
comment: Authored: Egan, SE, Orlic-Milacic, M, 2011-11-14
comment: Using Chinese hamster (Cricetulus griseus) ovarian cell lines deficient in different galactosyltransferases, Chen et al. established that beta-4-galactosyltransferase 1 (B4galt1) was necessary for galactosylation of beta 1,3-linked N-acetylglucosaminyl group added by fringe enzymes to O-linked fucosyl residues on Notch1. B4galt1-mediated galactosylation of Notch1 was necessary for fringe-induced modulation of Notch1 signaling. Since the genome of C. griseus has not been completely sequenced, it is possible that annotated C. griseus Notch isoforms do not represent full-length proteins. Annotated C. griseus Notch2 in particular seems truncated. Only those fucosylation and glycosylation sites that are conserved between Chinese hamster, mouse, human and rat are shown. Since the exact preference, if any, of fringe enzymes for Notch O-fucose sites is not known, the extension of an O-fucosyl residue at an unknown position is shown.
comment: Reviewed: Haw, R, 2012-02-06
comment: Edited: D'Eustachio, P, 2012-02-06
right: Glc,Gal-GlcNAc-Fuc-Pre-Notch
right: UDP

UnificationXref26
type: UnificationXref
id: GO:0000139
db: GENE ONTOLOGY

UnificationXref27
type: UnificationXref
id: G3I6Z6
db: UniProt

UnificationXref28
type: UnificationXref
id: MOD:00813
db: MOD

UnificationXref29
type: UnificationXref
id: MOD:00812
db: MOD

UnificationXref22
type: UnificationXref
id: 2317523
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2317523

oxysterol
type: SmallMolecule
entity reference: oxysterol
data source: Reactome
xref: UnificationXref16
xref: UnificationXref15
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 2317518

UDP-D-galactopyranose
type: SmallMolecule
entity reference: UDP-D-galactose [ChEBI:18307]
data source: Reactome
name: UDPgalactose
name: UDP-D-galactose
xref: UnificationXref46
xref: UnificationXref47
cellular location: Golgi lumen
comment: Reactome DB_ID: 735682
display name: UDP-Gal

UnificationXref23
type: UnificationXref
id: REACT_148178
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148178.1

UDP
type: SmallMolecule
entity reference: UDP
data source: Reactome
xref: UnificationXref60
xref: UnificationXref59
cellular location: Golgi lumen
comment: Reactome DB_ID: 205687

UnificationXref24
type: UnificationXref
id: 2317528
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2317528

SequenceSite198
type: SequenceSite
position status: EQUAL
sequence position: 251

CMP-sialic acid
type: SmallMolecule
entity reference: CMP-sialic acid
data source: Reactome
xref: UnificationXref71
xref: UnificationXref70
cellular location: Golgi lumen
comment: Reactome DB_ID: 727815
display name: CMP-SA

UnificationXref25
type: UnificationXref
id: REACT_147768
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_147768.1

SequenceSite199
type: SequenceSite
position status: EQUAL
sequence position: 291

Cytidine-5'-monophosphate
type: SmallMolecule
entity reference: CMP [ChEBI:17361]
data source: Reactome
name: cytidine 5'-monophosphate
name: cytidylic acid
xref: UnificationXref74
xref: UnificationXref73
cellular location: Golgi lumen
comment: Reactome DB_ID: 727810
display name: CMP

SequenceSite196
type: SequenceSite
position status: EQUAL
sequence position: 174

Cholesterol
type: SmallMolecule
entity reference: cholest-5-en-3beta-ol
data source: Reactome
xref: UnificationXref99
xref: UnificationXref100
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 193384

SequenceSite197
type: SequenceSite
position status: EQUAL
sequence position: 212

GDP-L-fucose
type: SmallMolecule
entity reference: GDP-L-fucose
data source: Reactome
xref: UnificationXref147
xref: UnificationXref146
cellular location: endoplasmic reticulum lumen
comment: Reactome DB_ID: 1463452
display name: GDP-Fuc

SequenceSite194
type: SequenceSite
position status: EQUAL
sequence position: 26

Guanosine 5'-Diphosphate
type: SmallMolecule
entity reference: Guanosine 5'-diphosphate
data source: Reactome
name: Guanosine Diphosphate
xref: UnificationXref173
xref: UnificationXref174
cellular location: endoplasmic reticulum lumen
comment: Reactome DB_ID: 1467290
display name: GDP

SequenceSite195
type: SequenceSite
position status: EQUAL
sequence position: 2470

SequenceSite192
type: SequenceSite
position status: EQUAL
sequence position: 1199

SequenceSite193
type: SequenceSite
position status: EQUAL
sequence position: 1316

SequenceSite190
type: SequenceSite
position status: EQUAL
sequence position: 1075

SequenceSite191
type: SequenceSite
position status: EQUAL
sequence position: 1161

UnificationXref32
type: UnificationXref
id: REACT_119944
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119944.1

UnificationXref31
type: UnificationXref
id: 2032277
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032277

UnificationXref30
type: UnificationXref
id: MOD:00804
db: MOD

UnificationXref35
type: UnificationXref
id: REACT_118874
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_118874.1

UnificationXref36
type: UnificationXref
id: G3HM56
db: UniProt

http://identifiers.org/pubmed/12202038
type: PublicationXref
id: 12202038
author: Espenshade, PJ
author: Brown, MS
author: Gong, Y
author: Aebersold, R
author: Yang, T
author: Wright, ME
author: Goldstein, JL
author: Yabe, D
db: Pubmed
title: Crucial step in cholesterol homeostasis: sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER
source: Cell 110:489-500
year: 2002

UnificationXref33
type: UnificationXref
id: G3HP78
db: UniProt

UnificationXref34
type: UnificationXref
id: 2032279
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032279

UnificationXref39
type: UnificationXref
id: G3HZC0
db: UniProt

UnificationXref37
type: UnificationXref
id: 2032281
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032281

UnificationXref38
type: UnificationXref
id: REACT_119427
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119427.1

UnificationXref41
type: UnificationXref
id: REACT_119494
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119494.1

UnificationXref40
type: UnificationXref
id: 2032283
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032283

UnificationXref43
type: UnificationXref
id: REACT_120031
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_120031.1

UnificationXref42
type: UnificationXref
id: 2032302
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032302

UnificationXref44
type: UnificationXref
id: GO:0005796
db: GENE ONTOLOGY

UnificationXref45
type: UnificationXref
id: CHEBI:18307
db: ChEBI

UnificationXref46
type: UnificationXref
id: 735682
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=735682

UnificationXref47
type: UnificationXref
id: REACT_23172
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_23172.2

UnificationXref48
type: UnificationXref
id: 2032269
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032269

UnificationXref49
type: UnificationXref
id: REACT_119960
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119960.1

KIAA0079
type: ProteinReference
name: UniProt:P53992 SEC24C
name: SEC24C
xref: UnificationXref122
comment: FUNCTION Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.SUBUNIT Interacts with SLC6A4 (By similarity). COPII is composed of at least five proteins: the Sec23/24 complex, the Sec13/31 complex and Sar1. SEC24C is capable of forming heterodimers with SEC24A. Interacts with TMED2 and TMED10.TISSUE SPECIFICITY Ubiquitous.SIMILARITY Belongs to the SEC23/SEC24 family. SEC24 subfamily.
organism: Homo sapiens

Glc,Gal-GlcNAc-Fuc-Pre-Notch
type: Protein
data source: Reactome
member physical entity: Glc,Gal-GlcNAc-Fuc-Pre-Notch2
member physical entity: Glc,Gal-GlcNAc-Fuc-Pre-Notch1
member physical entity: Glc,Gal-GlcNAc-Fuc-Pre-Notch3
member physical entity: Glc,Gal-GlcNAc-Fuc-Pre-Notch4
xref: UnificationXref57
xref: UnificationXref56
cellular location: Golgi membrane
comment: Reactome DB_ID: 2032300
comment: Converted from EntitySet in Reactome

Glc,GlcNAc-Fuc-Pre-Notch4
type: Protein
entity reference: Notch4
data source: Reactome
xref: UnificationXref40
xref: UnificationXref41
feature: ModificationFeature95
feature: ModificationFeature83
feature: ModificationFeature89
feature: ModificationFeature84
feature: ModificationFeature81
feature: ModificationFeature93
feature: ModificationFeature79
feature: ModificationFeature87
feature: ModificationFeature88
feature: ModificationFeature94
feature: ModificationFeature74
feature: ModificationFeature77
feature: ModificationFeature91
feature: ModificationFeature86
feature: ModificationFeature90
feature: ModificationFeature78
feature: ModificationFeature80
feature: FragmentFeature7
feature: ModificationFeature76
feature: ModificationFeature82
feature: ModificationFeature73
feature: ModificationFeature85
feature: ModificationFeature92
feature: ModificationFeature75
cellular location: Golgi membrane
comment: Reactome DB_ID: 2032283

Glc,GlcNAc-Fuc-Pre-Notch3
type: Protein
entity reference: UniProt:G3HM56 Notch3
data source: Reactome
xref: UnificationXref37
xref: UnificationXref38
feature: ModificationFeature67
feature: ModificationFeature69
feature: ModificationFeature52
feature: ModificationFeature60
feature: ModificationFeature51
feature: ModificationFeature66
feature: ModificationFeature57
feature: ModificationFeature64
feature: ModificationFeature54
feature: ModificationFeature61
feature: ModificationFeature49
feature: ModificationFeature50
feature: ModificationFeature65
feature: ModificationFeature68
feature: ModificationFeature70
feature: ModificationFeature48
feature: ModificationFeature47
feature: ModificationFeature62
feature: ModificationFeature55
feature: ModificationFeature46
feature: ModificationFeature58
feature: ModificationFeature71
feature: ModificationFeature56
feature: ModificationFeature59
feature: ModificationFeature63
feature: ModificationFeature72
feature: ModificationFeature53
feature: FragmentFeature6
cellular location: Golgi membrane
comment: Reactome DB_ID: 2032281

Glc,GlcNAc-Fuc-Pre-Notch2
type: Protein
entity reference: UniProt:G3HP78 Notch2
data source: Reactome
xref: UnificationXref35
xref: UnificationXref34
feature: ModificationFeature36
feature: ModificationFeature35
feature: ModificationFeature45
feature: ModificationFeature44
feature: ModificationFeature43
feature: ModificationFeature42
feature: ModificationFeature38
feature: ModificationFeature39
feature: ModificationFeature37
feature: ModificationFeature40
feature: ModificationFeature41
feature: FragmentFeature5
cellular location: Golgi membrane
comment: Reactome DB_ID: 2032279

Glc,GlcNAc-Fuc-Pre-Notch1
type: Protein
entity reference: UniProt:G3I6Z6 Notch1
data source: Reactome
xref: UnificationXref31
xref: UnificationXref32
feature: ModificationFeature29
feature: ModificationFeature32
feature: ModificationFeature9
feature: ModificationFeature20
feature: ModificationFeature33
feature: ModificationFeature21
feature: FragmentFeature4
feature: ModificationFeature16
feature: ModificationFeature31
feature: ModificationFeature26
feature: ModificationFeature24
feature: ModificationFeature8
feature: ModificationFeature25
feature: ModificationFeature4
feature: ModificationFeature17
feature: ModificationFeature19
feature: ModificationFeature2
feature: ModificationFeature6
feature: ModificationFeature10
feature: ModificationFeature5
feature: ModificationFeature30
feature: ModificationFeature15
feature: ModificationFeature13
feature: ModificationFeature3
feature: ModificationFeature22
feature: ModificationFeature14
feature: ModificationFeature7
feature: ModificationFeature11
feature: ModificationFeature23
feature: ModificationFeature27
feature: ModificationFeature34
feature: ModificationFeature28
feature: ModificationFeature12
feature: ModificationFeature1
feature: ModificationFeature18
cellular location: Golgi membrane
comment: Reactome DB_ID: 2032277

Glc,GlcNAc-Fuc-Pre-Notch
type: Protein
data source: Reactome
member physical entity: Glc,GlcNAc-Fuc-Pre-Notch3
member physical entity: Glc,GlcNAc-Fuc-Pre-Notch2
member physical entity: Glc,GlcNAc-Fuc-Pre-Notch1
member physical entity: Glc,GlcNAc-Fuc-Pre-Notch4
xref: UnificationXref42
xref: UnificationXref43
cellular location: Golgi membrane
comment: Reactome DB_ID: 2032302
comment: Converted from EntitySet in Reactome

INSI2_HUMAN
type: Protein
entity reference: INSIG2
data source: Reactome
name: Insulin-induced gene 2 protein
xref: UnificationXref18
xref: UnificationXref19
feature: FragmentFeature3
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1655736
display name: INSIG2

SCAP tetramer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry26
component: SCAP_CRIGR
xref: UnificationXref139
xref: UnificationXref138
cellular location: Golgi membrane
comment: Reactome DB_ID: 1655751

SCAP_CRIGR
type: Protein
entity reference: UniProt:P97260 SCAP
data source: Reactome
xref: UnificationXref5
xref: UnificationXref4
feature: FragmentFeature1
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1655763
display name: SCAP

SRBP2_HUMAN
type: Protein
entity reference: SREBP2
data source: Reactome
name: SREBP2
name: Sterol regulatory element-binding protein 2
name: SREBF2(1-1141)
xref: UnificationXref11
xref: UnificationXref10
feature: FragmentFeature2
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1655733
display name: SREBF2

SCAP:SREBF2
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry27
component stoichiometry: Stoichiometry25
component: SRBP2_HUMAN
component: SCAP tetramer
xref: UnificationXref141
xref: UnificationXref140
cellular location: Golgi membrane
display name: SCAP:SREBP2
comment: Reactome DB_ID: 1655759

SequenceSite231
type: SequenceSite
position status: EQUAL
sequence position: 1964

SequenceSite232
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite233
type: SequenceSite
position status: EQUAL
sequence position: 392

SequenceSite230
type: SequenceSite
position status: EQUAL
sequence position: 21

SequenceSite229
type: SequenceSite
position status: EQUAL
sequence position: 1141

SequenceSite228
type: SequenceSite
position status: EQUAL
sequence position: 1056

Notch3
type: ProteinReference
name: UniProt:Q61982 Notch3
xref: UnificationXref154
comment: FUNCTION Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May play a role during CNS development.SUBUNIT Interacts with PSMA1 (By similarity). Heterodimer of a C-terminal fragment N(TM) and a N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH3. Interacts with HIF1AN.TISSUE SPECIFICITY Proliferating neuroepithelium.DEVELOPMENTAL STAGE CNS development.PTM Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.PTM Phosphorylated.PTM Hydroxylated by HIF1AN.SIMILARITY Belongs to the NOTCH family.SIMILARITY Contains 5 ANK repeats.SIMILARITY Contains 34 EGF-like domains.SIMILARITY Contains 3 LNR (Lin/Notch) repeats.
organism: Mus musculus

SequenceSite222
type: SequenceSite
position status: EQUAL
sequence position: 702

UnificationXref99
type: UnificationXref
id: 193384
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=193384

SequenceSite223
type: SequenceSite
position status: EQUAL
sequence position: 740

SequenceSite220
type: SequenceSite
position status: EQUAL
sequence position: 486

SequenceSite221
type: SequenceSite
position status: EQUAL
sequence position: 600

http://identifiers.org/pubmed/15899885
type: PublicationXref
id: 15899885
author: Sun, LP
author: Brown, MS
author: Goldstein, JL
author: Li, L
db: Pubmed
title: Insig required for sterol-mediated inhibition of Scap/SREBP binding to COPII proteins in vitro
source: J Biol Chem 280:26483-90
year: 2005

SequenceSite226
type: SequenceSite
position status: EQUAL
sequence position: 940

SequenceSite227
type: SequenceSite
position status: EQUAL
sequence position: 978

SequenceSite224
type: SequenceSite
position status: EQUAL
sequence position: 778

SequenceSite225
type: SequenceSite
position status: EQUAL
sequence position: 817

UnificationXref90
type: UnificationXref
id: REACT_119779
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119779.1

UnificationXref92
type: UnificationXref
id: 2032997
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032997

SequenceSite219
type: SequenceSite
position status: EQUAL
sequence position: 448

UnificationXref91
type: UnificationXref
id: G3I3V5
db: UniProt

UnificationXref94
type: UnificationXref
id: 2033000
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2033000

SequenceSite218
type: SequenceSite
position status: EQUAL
sequence position: 366

UnificationXref93
type: UnificationXref
id: REACT_119098
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119098.1

SequenceSite217
type: SequenceSite
position status: EQUAL
sequence position: 328

UnificationXref96
type: UnificationXref
id: 2033005
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2033005

O-fucosyl-L-threonine
type: SequenceModificationVocabulary
xref: UnificationXref28

UnificationXref95
type: UnificationXref
id: REACT_119184
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119184.1

O-fucosyl-L-serine
type: SequenceModificationVocabulary
xref: UnificationXref29

UnificationXref98
type: UnificationXref
id: CHEBI:16113
db: ChEBI

UnificationXref97
type: UnificationXref
id: REACT_118591
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_118591.2

O-glucosyl-L-serine
type: SequenceModificationVocabulary
xref: UnificationXref30

UnificationXref69
type: UnificationXref
id: CHEBI:16556
db: ChEBI

UnificationXref68
type: UnificationXref
id: REACT_118586
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_118586.2

UnificationXref67
type: UnificationXref
id: 2032355
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032355

UnificationXref66
type: UnificationXref
id: MI:0355
db: MI

Catalysis3
type: Catalysis
data source: Reactome
controlled: Fucosylation of Pre-Notch by POFUT1
control type: ACTIVATION
xref: RelationshipXref5
xref: RelationshipXref6
controller: POFUT1

UnificationXref60
type: UnificationXref
id: REACT_26764
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_26764.1

UnificationXref61
type: UnificationXref
id: Q99PC2
db: UniProt

Catalysis1
type: Catalysis
data source: Reactome
controlled: Galactosylation of Pre-Notch
control type: ACTIVATION
xref: RelationshipXref2
xref: RelationshipXref1
controller: B4galt1 homodimer

Catalysis2
type: Catalysis
data source: Reactome
controlled: Sialylation of Pre-Notch
control type: ACTIVATION
xref: RelationshipXref4
xref: RelationshipXref3
controller: St3gal3/4/6

UnificationXref64
type: UnificationXref
id: 2023202
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2023202

UnificationXref65
type: UnificationXref
id: REACT_120179
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_120179.1

UnificationXref62
type: UnificationXref
id: 2023200
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2023200

UnificationXref63
type: UnificationXref
id: REACT_119496
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119496.1

UnificationXref56
type: UnificationXref
id: 2032300
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032300

UnificationXref55
type: UnificationXref
id: REACT_120308
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_120308.1

UnificationXref58
type: UnificationXref
id: CHEBI:17659
db: ChEBI

UnificationXref57
type: UnificationXref
id: REACT_119375
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119375.1

UnificationXref59
type: UnificationXref
id: 205687
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=205687

UnificationXref50
type: UnificationXref
id: 2032271
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032271

UnificationXref51
type: UnificationXref
id: REACT_119129
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119129.1

UnificationXref52
type: UnificationXref
id: 2032274
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032274

UnificationXref53
type: UnificationXref
id: REACT_119015
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119015.1

UnificationXref54
type: UnificationXref
id: 2032275
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032275

UnificationXref89
type: UnificationXref
id: 2032999
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032999

UnificationXref88
type: UnificationXref
id: Q80WL0
db: UniProt

UnificationXref86
type: UnificationXref
id: 2032998
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032998

UnificationXref87
type: UnificationXref
id: REACT_119275
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119275.1

UnificationXref84
type: UnificationXref
id: REACT_119448
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119448.1

UnificationXref85
type: UnificationXref
id: Q80WK9
db: UniProt

UnificationXref82
type: UnificationXref
id: REACT_119099
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119099.1

UnificationXref83
type: UnificationXref
id: 2032991
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032991

UnificationXref80
type: UnificationXref
id: REACT_119065
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119065.1

SREBP2
type: ProteinReference
name: UniProt:Q12772 SREBF2
name: BHLHD2
name: SREBF2
xref: UnificationXref9
comment: FUNCTION Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.SUBUNIT Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts (via C-terminus domain) with RNF139.TISSUE SPECIFICITY Ubiquitously expressed in adult and fetal tissues.PTM At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.SIMILARITY Belongs to the SREBP family.SIMILARITY Contains 1 bHLH (basic helix-loop-helix) domain.
organism: Homo sapiens

UnificationXref81
type: UnificationXref
id: 2032989
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032989

http://identifiers.org/pubmed/9242699
type: PublicationXref
id: 9242699
author: Brown, MS
author: Cheng, D
author: Sakai, J
author: Nohturfft, A
author: Ho, YK
author: Goldstein, JL
db: Pubmed
title: Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SREBPs) and SREBP cleavage-activating protein
source: J Biol Chem 272:20213-21
year: 1997

UnificationXref78
type: UnificationXref
id: REACT_120164
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_120164.1

UnificationXref77
type: UnificationXref
id: 2032981
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032981

UnificationXref79
type: UnificationXref
id: 2032987
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032987

UnificationXref73
type: UnificationXref
id: 727810
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=727810

UnificationXref74
type: UnificationXref
id: REACT_22895
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_22895.2

UnificationXref75
type: UnificationXref
id: 2032984
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2032984

UnificationXref76
type: UnificationXref
id: REACT_120048
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_120048.1

http://identifiers.org/pubmed/15692013
type: PublicationXref
id: 15692013
author: Lei, L
author: Xu, A
author: Irvine, KD
author: Okajima, T
db: Pubmed
title: Chaperone activity of protein O-fucosyltransferase 1 promotes notch receptor folding
source: Science 307:1599-603
year: 2005

UnificationXref70
type: UnificationXref
id: 727815
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=727815

I79_018123
type: ProteinReference
name: St3gal6
name: UniProt:G3I3V5 St3gal6
xref: UnificationXref91
organism: Cricetulus griseus

UnificationXref71
type: UnificationXref
id: REACT_22966
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_22966.2

UnificationXref72
type: UnificationXref
id: CHEBI:17361
db: ChEBI

Stoichiometry9
type: Stoichiometry
physical entity: INSIG2:oxysterol
stoichiometric coefficient: 4.0

Stoichiometry7
type: Stoichiometry
physical entity: SCAP tetramer
stoichiometric coefficient: 1.0

Stoichiometry8
type: Stoichiometry
physical entity: SRBP2_HUMAN
stoichiometric coefficient: 4.0

Stoichiometry5
type: Stoichiometry
physical entity: INSI2_HUMAN
stoichiometric coefficient: 1.0

Stoichiometry6
type: Stoichiometry
physical entity: INSIG2:oxysterol
stoichiometric coefficient: 4.0

Stoichiometry3
type: Stoichiometry
physical entity: SRBP2_HUMAN
stoichiometric coefficient: 4.0

Stoichiometry4
type: Stoichiometry
physical entity: oxysterol
stoichiometric coefficient: 1.0

Stoichiometry1
type: Stoichiometry
physical entity: SCAP_CRIGR
stoichiometric coefficient: 4.0

Stoichiometry2
type: Stoichiometry
physical entity: SCAP tetramer
stoichiometric coefficient: 1.0

http://identifiers.org/pubmed/12242332
type: PublicationXref
id: 12242332
author: Brown, MS
author: Goldstein, JL
author: Yabe, D
db: Pubmed
title: Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins
source: Proc Natl Acad Sci U S A 99:12753-8
year: 2002

Reactome
type: Provenance
comment: http://www.reactome.org

http://identifiers.org/pubmed/12486116
type: PublicationXref
id: 12486116
author: Shao, L
author: Moloney, DJ
author: Haltiwanger, RS
db: Pubmed
title: Fringe modifies O-fucose on mouse Notch1 at epidermal growth factor-like repeats within the ligand-binding site and the Abruptex region
source: J Biol Chem 278:7775-82
year: 2003

UniProt:G3HE95 Pofut1
type: ProteinReference
name: Pofut1
name: I79_008874
xref: UnificationXref175
comment: At the time of annotation, only a fragment of this protein was annotated in UniProt, under P83337
organism: Cricetulus griseus

http://identifiers.org/pubmed/15260976
type: PublicationXref
id: 15260976
author: Sun, LP
author: Brown, MS
author: Kwon, HJ
author: Goldstein, JL
author: Radhakrishnan, A
db: Pubmed
title: Direct binding of cholesterol to the purified membrane region of SCAP: mechanism for a sterol-sensing domain
source: Mol Cell 15:259-68
year: 2004

SequenceSite208
type: SequenceSite
position status: EQUAL
sequence position: 1182

SequenceSite209
type: SequenceSite
position status: EQUAL
sequence position: 1350

SequenceSite206
type: SequenceSite
position status: EQUAL
sequence position: 1099

SequenceSite207
type: SequenceSite
position status: EQUAL
sequence position: 1137

SequenceSite216
type: SequenceSite
position status: EQUAL
sequence position: 287

SequenceSite215
type: SequenceSite
position status: EQUAL
sequence position: 245

SequenceSite214
type: SequenceSite
position status: EQUAL
sequence position: 207

SequenceSite213
type: SequenceSite
position status: EQUAL
sequence position: 76

SequenceSite212
type: SequenceSite
position status: EQUAL
sequence position: 37

cholest-5-en-3beta-ol
type: SmallMoleculeReference
name: cholesterol
name: cholesterol [ChEBI:16113]
xref: UnificationXref98

SequenceSite211
type: SequenceSite
position status: EQUAL
sequence position: 2318

SequenceSite210
type: SequenceSite
position status: EQUAL
sequence position: 40

SequenceSite203
type: SequenceSite
position status: EQUAL
sequence position: 749

SequenceSite202
type: SequenceSite
position status: EQUAL
sequence position: 672

SequenceSite205
type: SequenceSite
position status: EQUAL
sequence position: 939

SequenceSite204
type: SequenceSite
position status: EQUAL
sequence position: 864

SequenceSite201
type: SequenceSite
position status: EQUAL
sequence position: 446

SequenceSite200
type: SequenceSite
position status: EQUAL
sequence position: 329

Notch4
type: ProteinReference
name: Int-3
name: UniProt:P31695 Notch4
name: Int3
xref: UnificationXref157
comment: FUNCTION Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May regulate branching morphogenesis in the developing vascular system.SUBUNIT Heterodimer of a C-terminal fragment N(TM) and a N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4.TISSUE SPECIFICITY Highly expressed in lung, moderately in heart kidney, and at lower levels in the ovary and skeletal muscle. A very low expression is seen in the brain, intestine, liver and testis.DEVELOPMENTAL STAGE Highly expressed in endothelial cells during embryonic development from 9.0 dpc.PTM Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.PTM Phosphorylated.DISEASE Note=Loss of the extracellular domain causes constitutive activation of the Notch protein, which leads to hyperproliferation of glandular epithelial tissues and development of mammary carcinomas.SIMILARITY Belongs to the NOTCH family.SIMILARITY Contains 5 ANK repeats.SIMILARITY Contains 29 EGF-like domains…
organism: Mus musculus

POFUT1
type: Protein
entity reference: UniProt:G3HE95 Pofut1
data source: Reactome
xref: UnificationXref176
xref: UnificationXref177
feature: FragmentFeature36
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1985676

Fucosylated Notch4 precursor
type: Protein
entity reference: Notch4
data source: Reactome
xref: UnificationXref168
xref: UnificationXref169
feature: ModificationFeature171
feature: ModificationFeature164
feature: ModificationFeature167
feature: ModificationFeature156
feature: ModificationFeature161
feature: ModificationFeature165
feature: ModificationFeature166
feature: ModificationFeature168
feature: ModificationFeature163
feature: ModificationFeature157
feature: FragmentFeature35
feature: ModificationFeature162
feature: ModificationFeature172
feature: ModificationFeature160
feature: ModificationFeature173
feature: ModificationFeature159
feature: ModificationFeature158
feature: ModificationFeature170
feature: ModificationFeature169
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1986568
display name: Fuc-Pre-Notch4

Fucosylated Notch3 precursor
type: Protein
entity reference: Notch3
data source: Reactome
xref: UnificationXref167
xref: UnificationXref166
feature: ModificationFeature146
feature: FragmentFeature34
feature: ModificationFeature150
feature: ModificationFeature152
feature: ModificationFeature153
feature: ModificationFeature154
feature: ModificationFeature144
feature: ModificationFeature143
feature: ModificationFeature147
feature: ModificationFeature155
feature: ModificationFeature142
feature: ModificationFeature145
feature: ModificationFeature149
feature: ModificationFeature151
feature: ModificationFeature148
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1986553
display name: Fuc-Pre-Notch3

Fucosylated Notch2 precursor
type: Protein
entity reference: UniProt:O35516 Notch2
data source: Reactome
xref: UnificationXref165
xref: UnificationXref164
feature: ModificationFeature123
feature: ModificationFeature140
feature: ModificationFeature136
feature: ModificationFeature127
feature: ModificationFeature125
feature: FragmentFeature33
feature: ModificationFeature124
feature: ModificationFeature133
feature: ModificationFeature126
feature: ModificationFeature141
feature: ModificationFeature139
feature: ModificationFeature132
feature: ModificationFeature129
feature: ModificationFeature130
feature: ModificationFeature131
feature: ModificationFeature134
feature: ModificationFeature128
feature: ModificationFeature135
feature: ModificationFeature138
feature: ModificationFeature137
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1986542
display name: Fuc-Pre-Notch2

Fucosylated Notch1 precursor
type: Protein
entity reference: Notch1
data source: Reactome
xref: UnificationXref163
xref: UnificationXref162
feature: FragmentFeature32
feature: ModificationFeature109
feature: ModificationFeature115
feature: ModificationFeature106
feature: ModificationFeature105
feature: ModificationFeature108
feature: ModificationFeature110
feature: ModificationFeature116
feature: ModificationFeature114
feature: ModificationFeature104
feature: ModificationFeature111
feature: ModificationFeature118
feature: ModificationFeature120
feature: ModificationFeature122
feature: ModificationFeature121
feature: ModificationFeature117
feature: ModificationFeature119
feature: ModificationFeature107
feature: ModificationFeature113
feature: ModificationFeature112
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1985704
display name: Fuc-Pre-Notch1

Stoichiometry20
type: Stoichiometry
physical entity: S24C_HUMAN
stoichiometric coefficient: 4.0

UnificationXref180
type: UnificationXref
id: 1655838
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655838

Stoichiometry24
type: Stoichiometry
physical entity: SRBP2_HUMAN
stoichiometric coefficient: 4.0

Stoichiometry23
type: Stoichiometry
physical entity: SAR1B_CRIGR
stoichiometric coefficient: 4.0

Stoichiometry22
type: Stoichiometry
physical entity: SC23A_MOUSE
stoichiometric coefficient: 4.0

UnificationXref181
type: UnificationXref
id: REACT_147890
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_147890.1

Stoichiometry21
type: Stoichiometry
physical entity: SCAP tetramer
stoichiometric coefficient: 1.0

Stoichiometry27
type: Stoichiometry
physical entity: SCAP tetramer
stoichiometric coefficient: 1.0

Stoichiometry28
type: Stoichiometry
physical entity: GDP-L-fucose
stoichiometric coefficient: 70.0

Stoichiometry25
type: Stoichiometry
physical entity: SRBP2_HUMAN
stoichiometric coefficient: 4.0

Stoichiometry26
type: Stoichiometry
physical entity: SCAP_CRIGR
stoichiometric coefficient: 4.0

UnificationXref176
type: UnificationXref
id: 1985676
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1985676

UnificationXref177
type: UnificationXref
id: REACT_119991
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119991.1

UnificationXref178
type: UnificationXref
id: 1986584
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1986584

Stoichiometry29
type: Stoichiometry
physical entity: Guanosine 5'-Diphosphate
stoichiometric coefficient: 70.0

UnificationXref179
type: UnificationXref
id: REACT_118648
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_118648.2

UnificationXref175
type: UnificationXref
id: G3HE95
db: UniProt

UnificationXref174
type: UnificationXref
id: REACT_119366
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119366.1

UnificationXref173
type: UnificationXref
id: 1467290
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1467290

UnificationXref172
type: UnificationXref
id: CHEBI:17552
db: ChEBI

Guanosine 5'-diphosphate
type: SmallMoleculeReference
name: GDP
name: Guanosine diphosphate
name: GDP [ChEBI:17552]
xref: UnificationXref172

UnificationXref171
type: UnificationXref
id: REACT_120130
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_120130.1

Stoichiometry11
type: Stoichiometry
physical entity: Cholesterol
stoichiometric coefficient: 4.0

UnificationXref170
type: UnificationXref
id: 1986582
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1986582

Stoichiometry10
type: Stoichiometry
physical entity: B4galt1
stoichiometric coefficient: 2.0

Stoichiometry13
type: Stoichiometry
physical entity: SREBP1A,1C,2
stoichiometric coefficient: 1.0

Stoichiometry12
type: Stoichiometry
physical entity: INSIG
stoichiometric coefficient: 4.0

Stoichiometry14
type: Stoichiometry
physical entity: SCAP tetramer
stoichiometric coefficient: 1.0

Stoichiometry15
type: Stoichiometry
physical entity: SREBP1A,1C,2
stoichiometric coefficient: 4.0

UnificationXref169
type: UnificationXref
id: REACT_119227
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119227.1

Stoichiometry16
type: Stoichiometry
physical entity: INSIG
stoichiometric coefficient: 4.0

Stoichiometry17
type: Stoichiometry
physical entity: Cholesterol
stoichiometric coefficient: 4.0

Stoichiometry18
type: Stoichiometry
physical entity: SCAP tetramer
stoichiometric coefficient: 1.0

UnificationXref167
type: UnificationXref
id: REACT_119295
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119295.1

UnificationXref168
type: UnificationXref
id: 1986568
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1986568

Stoichiometry19
type: Stoichiometry
physical entity: SCAP:cholesterol
stoichiometric coefficient: 1.0

CMP-sialic acid
type: SmallMoleculeReference
name: ChEBI:59434 CMP-sialic acid
xref: UnificationXref69

UnificationXref165
type: UnificationXref
id: REACT_119580
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119580.1

UnificationXref166
type: UnificationXref
id: 1986553
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1986553

Beta-1,4-galactosyltransferase 1
type: ProteinReference
name: UniProt:Q99PC2 B4galt1
name: B4galt1
name: beta4GalT-1
xref: UnificationXref61
organism: Cricetulus griseus

INSIG2
type: ProteinReference
name: UniProt:Q9Y5U4 INSIG2
xref: UnificationXref17
comment: FUNCTION Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR.SUBUNIT Binds to the SCAP-SREBF2 complex only in the presence of sterols. Interacts with RNF139.MISCELLANEOUS Does not require nuclear SREBPs for its expression. When nuclear SREBP activity is low, is the only form of INSIG present in the cell.SIMILARITY Belongs to the INSIG family.
organism: Homo sapiens

UnificationXref160
type: UnificationXref
id: 1984033
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1984033

UnificationXref162
type: UnificationXref
id: 1985704
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1985704

UnificationXref161
type: UnificationXref
id: REACT_118959
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_118959.1

UnificationXref164
type: UnificationXref
id: 1986542
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1986542

UnificationXref163
type: UnificationXref
id: REACT_119409
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119409.1

UnificationXref154
type: UnificationXref
id: Q61982
db: UniProt

UnificationXref155
type: UnificationXref
id: 1984032
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1984032

UnificationXref156
type: UnificationXref
id: REACT_119457
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119457.1

UnificationXref157
type: UnificationXref
id: P31695
db: UniProt

UnificationXref158
type: UnificationXref
id: 1984031
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1984031

UnificationXref159
type: UnificationXref
id: REACT_119852
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119852.1

UnificationXref153
type: UnificationXref
id: REACT_119738
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119738.1

UnificationXref152
type: UnificationXref
id: 1984029
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1984029

UnificationXref151
type: UnificationXref
id: O35516
db: UniProt

UnificationXref150
type: UnificationXref
id: REACT_119856
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119856.1

UnificationXref145
type: UnificationXref
id: CHEBI:17009
db: ChEBI

UnificationXref146
type: UnificationXref
id: 1463452
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1463452

UnificationXref143
type: UnificationXref
id: REACT_147893
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_147893.1

UnificationXref144
type: UnificationXref
id: GO:0005788
db: GENE ONTOLOGY

UnificationXref149
type: UnificationXref
id: 1984030
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1984030

UnificationXref147
type: UnificationXref
id: REACT_119248
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_119248.2

UnificationXref148
type: UnificationXref
id: Q01705
db: UniProt

UniProt:G3HM56 Notch3
type: ProteinReference
name: Notch3
name: I79_011804
xref: UnificationXref36
organism: Cricetulus griseus

http://identifiers.org/pubmed/12963821
type: PublicationXref
id: 12963821
author: Brown, MS
author: Adams, CM
author: Goldstein, JL
db: Pubmed
title: Cholesterol-induced conformational change in SCAP enhanced by Insig proteins and mimicked by cationic amphiphiles
source: Proc Natl Acad Sci U S A 100:10647-52
year: 2003

UnificationXref139
type: UnificationXref
id: REACT_148424
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148424.1

UnificationXref138
type: UnificationXref
id: 1655751
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655751

UnificationXref137
type: UnificationXref
id: REACT_148491
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148491.1

UnificationXref136
type: UnificationXref
id: 1655750
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655750

UnificationXref135
type: UnificationXref
id: REACT_148180
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148180.2

UnificationXref134
type: UnificationXref
id: 1655747
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655747

UnificationXref133
type: UnificationXref
id: REACT_148034
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148034.1

UnificationXref132
type: UnificationXref
id: 1655768
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655768

UnificationXref141
type: UnificationXref
id: REACT_148336
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148336.1

UnificationXref142
type: UnificationXref
id: 1655840
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655840

UnificationXref140
type: UnificationXref
id: 1655759
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655759

UnificationXref129
type: UnificationXref
id: Q9QVY3
db: UniProt

UnificationXref126
type: UnificationXref
id: Q01405
db: UniProt

UnificationXref125
type: UnificationXref
id: 10090
db: NCBI Taxonomy

UnificationXref128
type: UnificationXref
id: REACT_148081
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148081.1

UnificationXref127
type: UnificationXref
id: 1655767
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655767

UnificationXref122
type: UnificationXref
id: P53992
db: UniProt

UnificationXref121
type: UnificationXref
id: REACT_147840
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_147840.1

UnificationXref124
type: UnificationXref
id: REACT_12899
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_12899.2

UnificationXref123
type: UnificationXref
id: 203987
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=203987

UnificationXref130
type: UnificationXref
id: 1655715
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655715

UnificationXref131
type: UnificationXref
id: REACT_147932
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_147932.1

UnificationXref119
type: UnificationXref
id: REACT_147975
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_147975.1

UnificationXref118
type: UnificationXref
id: 1655727
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655727

UnificationXref113
type: UnificationXref
id: REACT_148091
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148091.2

UnificationXref112
type: UnificationXref
id: 1655730
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655730

UnificationXref111
type: UnificationXref
id: REACT_148171
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148171.2

UnificationXref110
type: UnificationXref
id: 1655761
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655761

UnificationXref117
type: UnificationXref
id: REACT_148574
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148574.1

UnificationXref116
type: UnificationXref
id: 1655758
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655758

UnificationXref115
type: UnificationXref
id: REACT_148327
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148327.1

UnificationXref114
type: UnificationXref
id: 2326883
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2326883

UnificationXref120
type: UnificationXref
id: 2317526
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2317526

UnificationXref108
type: UnificationXref
id: REACT_148375
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148375.2

UnificationXref107
type: UnificationXref
id: 1655757
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655757

UnificationXref109
type: UnificationXref
id: P36956-3
db: UniProt

http://identifiers.org/pubmed/11524432
type: PublicationXref
id: 11524432
author: Shi, S
author: Shao, L
author: Wang, Y
author: Harris, RJ
author: Stanley, P
author: Spellman, MW
author: Haltiwanger, RS
db: Pubmed
title: Modification of epidermal growth factor-like repeats with O-fucose. Molecular cloning and expression of a novel GDP-fucose protein O-fucosyltransferase
source: J Biol Chem 276:40338-45
year: 2001

UnificationXref100
type: UnificationXref
id: REACT_11015
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_11015.1

UnificationXref102
type: UnificationXref
id: 1655735
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655735

UnificationXref101
type: UnificationXref
id: O15503
db: UniProt

UnificationXref104
type: UnificationXref
id: 1655749
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655749

UnificationXref103
type: UnificationXref
id: REACT_148184
db: Reactome
id version: 2
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148184.2

UnificationXref106
type: UnificationXref
id: P36956-1
db: UniProt

UnificationXref105
type: UnificationXref
id: REACT_148176
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148176.1

endoplasmic reticulum membrane
type: CellularLocationVocabulary
xref: UnificationXref1

endoplasmic reticulum lumen
type: CellularLocationVocabulary
xref: UnificationXref144

CMP [ChEBI:17361]
type: SmallMoleculeReference
name: Cytidine-5'-monophosphate
name: Cytidylic acid
name: CMP
xref: UnificationXref72

http://identifiers.org/pubmed/21454655
type: PublicationXref
id: 21454655
author: Wang, ML
author: Brown, MS
author: Zhang, Y
author: Kwon, HJ
author: Motamed, M
author: Goldstein, JL
author: Seemann, J
db: Pubmed
title: Identification of luminal Loop 1 of Scap protein as the sterol sensor that maintains cholesterol homeostasis
source: J Biol Chem 286:18002-12
year: 2011

http://identifiers.org/pubmed/17428919
type: PublicationXref
id: 17428919
author: Sun, LP
author: Brown, MS
author: Goldstein, JL
author: Seemann, J
db: Pubmed
title: Sterol-regulated transport of SREBPs from endoplasmic reticulum to Golgi: Insig renders sorting signal in Scap inaccessible to COPII proteins
source: Proc Natl Acad Sci U S A 104:6519-26
year: 2007

http://identifiers.org/pubmed/10935626
type: PublicationXref
id: 10935626
author: Shao, L
author: Wang, Y
author: Johnston, SH
author: Panin, VM
author: Moloney, DJ
author: Vogt, TF
author: Wilson, R
author: Stanley, P
author: Irvine, KD
author: Chen, J
author: Haltiwanger, RS
db: Pubmed
title: Fringe is a glycosyltransferase that modifies Notch
source: Nature 406:369-75
year: 2000

http://identifiers.org/pubmed/15452130
type: PublicationXref
id: 15452130
author: Brown, MS
author: Reitz, J
author: Adams, CM
author: Feramisco, JD
author: De Brabander, JK
author: Goldstein, JL
author: Li, L
db: Pubmed
title: Cholesterol and 25-hydroxycholesterol inhibit activation of SREBPs by different mechanisms, both involving SCAP and Insigs
source: J Biol Chem 279:52772-80
year: 2004

Golgi lumen
type: CellularLocationVocabulary
xref: UnificationXref44

http://identifiers.org/pubmed/19041766
type: PublicationXref
id: 19041766
author: Brown, MS
author: Goldstein, JL
author: McDonald, JG
author: Radhakrishnan, A
db: Pubmed
title: Switch-like control of SREBP-2 transport triggered by small changes in ER cholesterol: a delicate balance
source: Cell Metab 8:512-21
year: 2008

SequenceSite177
type: SequenceSite
position status: EQUAL
sequence position: 158

http://identifiers.org/pubmed/17428920
type: PublicationXref
id: 17428920
author: Brown, MS
author: Kwon, Hyock Joo
author: Radhakrishnan, Arun
author: Ikeda, Yukio
author: Goldstein, JL
db: Pubmed
title: Sterol-regulated transport of SREBPs from endoplasmic reticulum to Golgi: oxysterols block transport by binding to Insig
source: Proc. Natl. Acad. Sci. U.S.A. 104:6511-8
year: 2007

SequenceSite176
type: SequenceSite
position status: EQUAL
sequence position: 120

SequenceSite179
type: SequenceSite
position status: EQUAL
sequence position: 233

Same Catalyst Activity
type: RelationshipTypeVocabulary

SequenceSite178
type: SequenceSite
position status: EQUAL
sequence position: 197

SequenceSite173
type: SequenceSite
position status: EQUAL
sequence position: 19

SequenceSite172
type: SequenceSite
position status: EQUAL
sequence position: 1402

SequenceSite175
type: SequenceSite
position status: EQUAL
sequence position: 78

SequenceSite174
type: SequenceSite
position status: EQUAL
sequence position: 2531

SequenceSite171
type: SequenceSite
position status: EQUAL
sequence position: 1362

SequenceSite170
type: SequenceSite
position status: EQUAL
sequence position: 1243

Sec23
type: ProteinReference
name: UniProt:Q01405 Sec23a
name: Sec23r
name: Sec23a
xref: UnificationXref126
comment: SUBUNIT COPII is composed of at least five proteins: the Sec23/24 complex, the Sec13/31 complex and Sar1. Interacts with SEC23IP. Interacts with HTR4. Interacts with SEC16A (By similarity). Interacts with SLC6A4.TISSUE SPECIFICITY High levels in brain and fibroblasts.SIMILARITY Belongs to the SEC23/SEC24 family. SEC23 subfamily.
organism: Mus musculus

UniProt:G3I6Z6 Notch1
type: ProteinReference
name: Notch1
name: I79_019276
xref: UnificationXref27
organism: Cricetulus griseus

RelationshipXref5
type: RelationshipXref
id: GO:0046922
db: GENE ONTOLOGY
relationship type: gene ontology term for cellular function

RelationshipXref6
type: RelationshipXref
id: 1986586
db: Reactome Database ID Release 45
relationship type: Same Catalyst Activity
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1986586

RelationshipXref3
type: RelationshipXref
id: GO:0008373
db: GENE ONTOLOGY
relationship type: gene ontology term for cellular function

RelationshipXref4
type: RelationshipXref
id: 2033002
db: Reactome Database ID Release 45
relationship type: Same Catalyst Activity
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2033002

SequenceSite169
type: SequenceSite
position status: EQUAL
sequence position: 1197

RelationshipXref2
type: RelationshipXref
id: 2029554
db: Reactome Database ID Release 45
relationship type: Same Catalyst Activity
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=2029554

SequenceSite168
type: SequenceSite
position status: EQUAL
sequence position: 1159

RelationshipXref1
type: RelationshipXref
id: GO:0003831
db: GENE ONTOLOGY
relationship type: gene ontology term for cellular function

SequenceSite167
type: SequenceSite
position status: EQUAL
sequence position: 1035

SequenceSite166
type: SequenceSite
position status: EQUAL
sequence position: 997

SequenceSite165
type: SequenceSite
position status: EQUAL
sequence position: 921

SequenceSite164
type: SequenceSite
position status: EQUAL
sequence position: 883

SequenceSite163
type: SequenceSite
position status: EQUAL
sequence position: 805

UniProt:O35516 Notch2
type: ProteinReference
name: Notch2
xref: UnificationXref151
comment: FUNCTION Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation.SUBUNIT Heterodimer of a C-terminal fragment N(TM) and an N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH2. Interacts with RELA/p65. Interacts with HIF1AN.TISSUE SPECIFICITY Expressed in the brain, liver, kidney, neuroepithelia, somites, optic vesicles and branchial arches, but not heart.DEVELOPMENTAL STAGE Expressed in the embryonic ventricular zone, the postnatal ependymal cells, and the choroid plexus throughout embryonic and postnatal development.PTM Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.PTM Hydroxylated by HIF1AN.SIMILARITY Belongs to the NOTCH family.SIMILARITY Contains 6 ANK re…
organism: Mus musculus

SequenceSite162
type: SequenceSite
position status: EQUAL
sequence position: 767

SequenceSite161
type: SequenceSite
position status: EQUAL
sequence position: 692

SequenceSite160
type: SequenceSite
position status: EQUAL
sequence position: 617

SequenceSite155
type: SequenceSite
position status: EQUAL
sequence position: 116

SequenceSite154
type: SequenceSite
position status: EQUAL
sequence position: 73

SequenceSite157
type: SequenceSite
position status: EQUAL
sequence position: 311

SequenceSite156
type: SequenceSite
position status: EQUAL
sequence position: 232

http://identifiers.org/pubmed/11707585
type: PublicationXref
id: 11707585
author: Moloney, DJ
author: Stanley, P
author: Chen, J
db: Pubmed
title: Fringe modulation of Jagged1-induced Notch signaling requires the action of beta 4galactosyltransferase-1
source: Proc Natl Acad Sci U S A 98:13716-21
year: 2001

SequenceSite159
type: SequenceSite
position status: EQUAL
sequence position: 466

SequenceSite158
type: SequenceSite
position status: EQUAL
sequence position: 349

SequenceSite151
type: SequenceSite
position status: EQUAL
sequence position: 2318

SequenceSite150
type: SequenceSite
position status: EQUAL
sequence position: 40

SequenceSite153
type: SequenceSite
position status: EQUAL
sequence position: 1964

SequenceSite152
type: SequenceSite
position status: EQUAL
sequence position: 21

SCAP tetramer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry1
component: SCAP_CRIGR
xref: UnificationXref6
xref: UnificationXref7
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1655722

INSIG2:oxysterol
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry4
component stoichiometry: Stoichiometry5
component: INSI2_HUMAN
component: oxysterol
xref: UnificationXref21
xref: UnificationXref20
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 2317521

SCAP:SREBF2
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry3
component stoichiometry: Stoichiometry2
component: SCAP tetramer
component: SRBP2_HUMAN
xref: UnificationXref12
xref: UnificationXref13
cellular location: endoplasmic reticulum membrane
display name: SCAP:SREBP2
comment: Reactome DB_ID: 2317524

SREBF1A/1C/2:SCAP
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry13
component stoichiometry: Stoichiometry14
component: SCAP tetramer
component: SREBP1A,1C,2
xref: UnificationXref115
xref: UnificationXref114
cellular location: endoplasmic reticulum membrane
display name: SREBP1A/1C/2:SCAP
comment: Reactome DB_ID: 2326883

SREBP1A/1C/2:SCAP:cholesterol:INSIG
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry16
component stoichiometry: Stoichiometry15
component stoichiometry: Stoichiometry19
component: SREBP1A,1C,2
component: SCAP:cholesterol
component: INSIG
xref: UnificationXref118
xref: UnificationXref119
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1655727

SREBF2:SCAP:INSIG2:oxysterol
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry9
component stoichiometry: Stoichiometry8
component stoichiometry: Stoichiometry7
component: SCAP tetramer
component: INSIG2:oxysterol
component: SRBP2_HUMAN
xref: UnificationXref23
xref: UnificationXref22
cellular location: endoplasmic reticulum membrane
display name: SREBP2:SCAP:INSIG2:oxysterol
comment: Reactome DB_ID: 2317523

B4galt1 homodimer
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry10
component: B4galt1
xref: UnificationXref65
xref: UnificationXref64
cellular location: Golgi membrane
comment: Reactome DB_ID: 2023202

SCAP:cholesterol
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry17
component stoichiometry: Stoichiometry18
component: SCAP tetramer
component: Cholesterol
xref: UnificationXref117
xref: UnificationXref116
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1655758

SREBP2:SCAP:CopII Coat
type: Complex
data source: Reactome
component stoichiometry: Stoichiometry22
component stoichiometry: Stoichiometry24
component stoichiometry: Stoichiometry23
component stoichiometry: Stoichiometry20
component stoichiometry: Stoichiometry21
component: SC23A_MOUSE
component: SCAP tetramer
component: S24C_HUMAN
component: SAR1B_CRIGR
component: SRBP2_HUMAN
xref: UnificationXref133
xref: UnificationXref132
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1655768

SequenceSite146
type: SequenceSite
position status: EQUAL
sequence position: 19

SequenceSite145
type: SequenceSite
position status: EQUAL
sequence position: 1276

SequenceSite144
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite143
type: SequenceSite
position status: EQUAL
sequence position: 1141

SAR1
type: ProteinReference
name: SARA2
name: SAR1B
name: UniProt:Q9QVY3 SAR1B
xref: UnificationXref129
comment: FUNCTION Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex.SUBUNIT Homodimer. Binds PREB. Part of the COPII coat complex. Binds to the cytoplasmic tails of target proteins in the endoplasmic reticulum.SIMILARITY Belongs to the small GTPase superfamily. SAR1 family.
organism: Cricetulus griseus

SequenceSite149
type: SequenceSite
position status: EQUAL
sequence position: 2470

SequenceSite148
type: SequenceSite
position status: EQUAL
sequence position: 26

SequenceSite147
type: SequenceSite
position status: EQUAL
sequence position: 2531

UniProt:Q80WK9 St3gal3
type: ProteinReference
name: St3gal3
xref: UnificationXref85
organism: Cricetulus griseus

SequenceSite142
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite141
type: SequenceSite
position status: EQUAL
sequence position: 198

SequenceSite140
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite129
type: SequenceSite
position status: EQUAL
sequence position: 320

UniProt:Q80WL0 St3gal4
type: ProteinReference
name: St3gal4
xref: UnificationXref88
organism: Cricetulus griseus

SequenceSite130
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite131
type: SequenceSite
position status: EQUAL
sequence position: 277

http://identifiers.org/pubmed/21464368
type: PublicationXref
id: 21464368
author: Lowe, JB
author: Wang, W
author: Yao, D
author: Xin, W
author: Gerson, S
author: Wei, L
author: Yan, Q
author: Huang, Y
author: Stanley, P
author: Zhou, L
author: Huang, X
db: Pubmed
title: Protein O-fucosyltransferase 1 (Pofut1) regulates lymphoid and myeloid homeostasis through modulation of Notch receptor ligand interactions
source: Blood 117:5652-62
year: 2011

SequenceSite136
type: SequenceSite
position status: EQUAL
sequence position: 1

Notch1
type: ProteinReference
name: UniProt:Q01705 Notch1
name: Motch
xref: UnificationXref148
comment: FUNCTION Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A.TISSUE SPECIFICITY Highly expressed in the brain, lung and thymus. Expressed at lower levels in the spleen, bone-marrow, spinal cord, eyes, mammary gland, liver, intestine, skeletal muscle, kidney and heart. In the hair follicle, highly expressed exclusively in the epithelial compartment.DEVELOPMENTAL STAGE First detected in the mesoderm at 7.5 dpc By 8.5 dpc highly expressed in presomitic mesoderm, mesenchyme and endothelial cells, while much lower levels are seen in the neuroepithelium. Between 9.5-10.5 dpc expressed at high levels in the neuroepithelium. At 13.5 dpc expressed in the surface ectoderm, eye and developing whisker follicles. Hair follicle matrix cells expression starts as different cell types become distinguishable in the developing follicle. Expression persists throughout the growth phase of the follicle and maintains the same expression profile in the second hair cycle. The cells in the follicle that undergo a phase of high level expr…
organism: Mus musculus

SequenceSite137
type: SequenceSite
position status: EQUAL
sequence position: 1094

SequenceSite138
type: SequenceSite
position status: EQUAL
sequence position: 1

http://identifiers.org/pubmed/12842885
type: PublicationXref
id: 12842885
author: Brown, MS
author: Dobrosotskaya, IY
author: Rawson, RB
author: Goldstein, JL
db: Pubmed
title: Reconstitution of sterol-regulated endoplasmic reticulum-to-Golgi transport of SREBP-2 in insect cells by co-expression of mammalian SCAP and Insigs
source: J Biol Chem 278:35837-43
year: 2003

SequenceSite139
type: SequenceSite
position status: EQUAL
sequence position: 765

SequenceSite132
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite133
type: SequenceSite
position status: EQUAL
sequence position: 1147

SequenceSite134
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite135
type: SequenceSite
position status: EQUAL
sequence position: 1147

http://identifiers.org/pubmed/12193656
type: PublicationXref
id: 12193656
author: Espenshade, PJ
author: Li, WP
author: Yabe, D
db: Pubmed
title: Sterols block binding of COPII proteins to SCAP, thereby controlling SCAP sorting in ER
source: Proc Natl Acad Sci U S A 99:11694-9
year: 2002

SequenceSite119
type: SequenceSite
position status: EQUAL
sequence position: 1521

SequenceSite118
type: SequenceSite
position status: EQUAL
sequence position: 1

http://identifiers.org/pubmed/11530204
type: PublicationXref
id: 11530204
author: Delannoy, P
author: Julien, S
author: Vallejo-Ruiz, V
author: Krzewinski-Recchi, MA
author: Harduin-Lepers, A
author: Samyn-Petit, B
db: Pubmed
title: The human sialyltransferase family
source: Biochimie 83:727-37
year: 2001

SequenceSite120
type: SequenceSite
position status: EQUAL
sequence position: 1

http://identifiers.org/pubmed/18347015
type: PublicationXref
id: 18347015
author: Shi, S
author: Stahl, M
author: Ge, C
author: Uemura, K
author: Stanley, P
author: Tashima, Y
db: Pubmed
title: Roles of Pofut1 and O-fucose in mammalian Notch signaling
source: J Biol Chem 283:13638-51
year: 2008

SequenceSite127
type: SequenceSite
position status: EQUAL
sequence position: 334

SequenceSite128
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite125
type: SequenceSite
position status: EQUAL
sequence position: 170

SequenceSite126
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite123
type: SequenceSite
position status: EQUAL
sequence position: 1797

SequenceSite124
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite121
type: SequenceSite
position status: EQUAL
sequence position: 2181

SequenceSite122
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite108
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite107
type: SequenceSite
position status: EQUAL
sequence position: 2412

SequenceSite109
type: SequenceSite
position status: EQUAL
sequence position: 1521

SequenceSite110
type: SequenceSite
position status: EQUAL
sequence position: 1

http://identifiers.org/pubmed/15728349
type: PublicationXref
id: 15728349
author: Brown, MS
author: Reitz, J
author: Feramisco, JD
author: Goldstein, JL
author: Ikeda, Y
author: Radhakrishnan, A
db: Pubmed
title: Intramembrane aspartic acid in SCAP protein governs cholesterol-induced conformational change
source: Proc Natl Acad Sci U S A 102:3242-7
year: 2005

SequenceSite111
type: SequenceSite
position status: EQUAL
sequence position: 2181

SequenceSite112
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite113
type: SequenceSite
position status: EQUAL
sequence position: 1797

SequenceSite114
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite115
type: SequenceSite
position status: EQUAL
sequence position: 393

SequenceSite116
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite117
type: SequenceSite
position status: EQUAL
sequence position: 2412

SequenceSite101
type: SequenceSite
position status: EQUAL
sequence position: 696

SequenceSite102
type: SequenceSite
position status: EQUAL
sequence position: 734

SequenceSite100
type: SequenceSite
position status: EQUAL
sequence position: 594

SequenceSite105
type: SequenceSite
position status: EQUAL
sequence position: 1797

SequenceSite106
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite103
type: SequenceSite
position status: EQUAL
sequence position: 1004

SequenceSite104
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite85
type: SequenceSite
position status: EQUAL
sequence position: 248

SequenceSite84
type: SequenceSite
position status: EQUAL
sequence position: 210

SequenceSite87
type: SequenceSite
position status: EQUAL
sequence position: 330

SequenceSite86
type: SequenceSite
position status: EQUAL
sequence position: 289

SequenceSite81
type: SequenceSite
position status: EQUAL
sequence position: 2181

SequenceSite80
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite83
type: SequenceSite
position status: EQUAL
sequence position: 79

SequenceSite82
type: SequenceSite
position status: EQUAL
sequence position: 40

Notch4
type: ProteinReference
name: UniProt:G3HZC0 Notch4
name: I79_016419
xref: UnificationXref39
organism: Cricetulus griseus

SequenceSite89
type: SequenceSite
position status: EQUAL
sequence position: 450

SequenceSite88
type: SequenceSite
position status: EQUAL
sequence position: 368

SequenceSite98
type: SequenceSite
position status: EQUAL
sequence position: 480

SequenceSite97
type: SequenceSite
position status: EQUAL
sequence position: 1097

SequenceSite96
type: SequenceSite
position status: EQUAL
sequence position: 1012

SequenceSite95
type: SequenceSite
position status: EQUAL
sequence position: 942

SequenceSite94
type: SequenceSite
position status: EQUAL
sequence position: 819

SequenceSite93
type: SequenceSite
position status: EQUAL
sequence position: 742

SequenceSite92
type: SequenceSite
position status: EQUAL
sequence position: 704

SequenceSite91
type: SequenceSite
position status: EQUAL
sequence position: 602

SequenceSite99
type: SequenceSite
position status: EQUAL
sequence position: 556

SequenceSite90
type: SequenceSite
position status: EQUAL
sequence position: 488

SequenceSite61
type: SequenceSite
position status: EQUAL
sequence position: 750

SequenceSite60
type: SequenceSite
position status: EQUAL
sequence position: 673

SequenceSite63
type: SequenceSite
position status: EQUAL
sequence position: 940

SequenceSite62
type: SequenceSite
position status: EQUAL
sequence position: 865

SequenceSite65
type: SequenceSite
position status: EQUAL
sequence position: 1138

SequenceSite64
type: SequenceSite
position status: EQUAL
sequence position: 1100

SequenceSite67
type: SequenceSite
position status: EQUAL
sequence position: 86

SequenceSite66
type: SequenceSite
position status: EQUAL
sequence position: 1183

SequenceSite69
type: SequenceSite
position status: EQUAL
sequence position: 359

SequenceSite68
type: SequenceSite
position status: EQUAL
sequence position: 127

Stoichiometry30
type: Stoichiometry
physical entity: S24C_HUMAN
stoichiometric coefficient: 4.0

Stoichiometry31
type: Stoichiometry
physical entity: SAR1B_CRIGR
stoichiometric coefficient: 4.0

Stoichiometry32
type: Stoichiometry
physical entity: SC23A_MOUSE
stoichiometric coefficient: 4.0

SequenceSite72
type: SequenceSite
position status: EQUAL
sequence position: 515

SequenceSite71
type: SequenceSite
position status: EQUAL
sequence position: 477

SequenceSite70
type: SequenceSite
position status: EQUAL
sequence position: 439

SequenceSite76
type: SequenceSite
position status: EQUAL
sequence position: 742

SequenceSite75
type: SequenceSite
position status: EQUAL
sequence position: 665

SequenceSite74
type: SequenceSite
position status: EQUAL
sequence position: 628

SequenceSite73
type: SequenceSite
position status: EQUAL
sequence position: 590

SequenceSite79
type: SequenceSite
position status: EQUAL
sequence position: 1130

SequenceSite78
type: SequenceSite
position status: EQUAL
sequence position: 970

SequenceSite77
type: SequenceSite
position status: EQUAL
sequence position: 895

oxysterol
type: SmallMoleculeReference
name: oxysterols
name: oxysterol [ChEBI:53030]
xref: UnificationXref14

SequenceSite48
type: SequenceSite
position status: EQUAL
sequence position: 369

SequenceSite49
type: SequenceSite
position status: EQUAL
sequence position: 82

SequenceSite44
type: SequenceSite
position status: EQUAL
sequence position: 90

SequenceSite45
type: SequenceSite
position status: EQUAL
sequence position: 128

SequenceSite46
type: SequenceSite
position status: EQUAL
sequence position: 214

SequenceSite47
type: SequenceSite
position status: EQUAL
sequence position: 252

SequenceSite40
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite41
type: SequenceSite
position status: EQUAL
sequence position: 2412

SequenceSite42
type: SequenceSite
position status: EQUAL
sequence position: 14

SequenceSite43
type: SequenceSite
position status: EQUAL
sequence position: 52

St3gal3/4/6
type: Protein
data source: Reactome
member physical entity: St3gal3
member physical entity: St3gal4
member physical entity: St3gal6
xref: UnificationXref94
xref: UnificationXref95
cellular location: Golgi membrane
comment: Reactome DB_ID: 2033000
comment: Converted from EntitySet in Reactome

SREBP1
type: ProteinReference
name: BHLHD1
name: SREBF1
name: UniProt:P36956-3 SREBF1
xref: UnificationXref109
comment: FUNCTION Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').SUBUNIT Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA.TISSUE SPECIFICITY Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.PTM At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.SIMILARITY Belongs to the SREBP family.SIMILARITY Contains 1 bHLH (basic helix-loop-helix) domain.
organism: Homo sapiens

St3gal3
type: Protein
entity reference: UniProt:Q80WK9 St3gal3
data source: Reactome
xref: UnificationXref86
xref: UnificationXref87
feature: FragmentFeature17
cellular location: Golgi membrane
comment: Reactome DB_ID: 2032998

St3gal4
type: Protein
entity reference: UniProt:Q80WL0 St3gal4
data source: Reactome
xref: UnificationXref90
xref: UnificationXref89
feature: FragmentFeature18
cellular location: Golgi membrane
comment: Reactome DB_ID: 2032999

SREBP1
type: ProteinReference
name: BHLHD1
name: SREBF1
name: UniProt:P36956-1 SREBF1
xref: UnificationXref106
comment: FUNCTION Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').SUBUNIT Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA.TISSUE SPECIFICITY Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.PTM At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.SIMILARITY Belongs to the SREBP family.SIMILARITY Contains 1 bHLH (basic helix-loop-helix) domain.
organism: Homo sapiens

St3gal6
type: Protein
entity reference: I79_018123
data source: Reactome
xref: UnificationXref93
xref: UnificationXref92
feature: FragmentFeature19
cellular location: Golgi membrane
comment: Reactome DB_ID: 2032997

INSIG
type: Protein
data source: Reactome
member physical entity: INSI1_HUMAN
member physical entity: INSI2_HUMAN
xref: UnificationXref104
xref: UnificationXref105
cellular location: endoplasmic reticulum membrane
comment: Converted from EntitySet in Reactome
comment: Reactome DB_ID: 1655749

INSI1_HUMAN
type: Protein
entity reference: INSIG1
data source: Reactome
name: Insulin-induced gene 1 protein
xref: UnificationXref103
xref: UnificationXref102
feature: FragmentFeature20
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1655735
display name: INSIG1

SREBP1A,1C,2
type: Protein
data source: Reactome
member physical entity: SRBP1_HUMAN
member physical entity: SRBP2_HUMAN
xref: UnificationXref112
xref: UnificationXref113
cellular location: endoplasmic reticulum membrane
comment: Converted from EntitySet in Reactome
comment: Reactome DB_ID: 1655730
display name: SREBF1A,1C,2

SRBP1_HUMAN
type: Protein
entity reference: SREBP1
data source: Reactome
name: SREBF1 isoform 1
name: SREBF1-1
name: Sterol regulatory element-binding protein 1A
name: SREBP1A
xref: UnificationXref107
xref: UnificationXref108
feature: FragmentFeature21
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1655757
display name: SREBF1A

SRBP1_HUMAN
type: Protein
entity reference: SREBP1
data source: Reactome
name: Sterol regulatory element-binding protein 1C
name: SREBF1 isoform 3
name: SREBP1C
name: SREBF1-3
xref: UnificationXref110
xref: UnificationXref111
feature: FragmentFeature22
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1655761
display name: SREBF1C

S24C_HUMAN
type: Protein
entity reference: KIAA0079
data source: Reactome
name: Protein transport protein Sec24C
xref: UnificationXref124
xref: UnificationXref123
feature: FragmentFeature23
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 203987
display name: SEC24C

gene ontology term for cellular function
type: RelationshipTypeVocabulary
xref: UnificationXref66

SequenceSite59
type: SequenceSite
position status: EQUAL
sequence position: 447

SequenceSite57
type: SequenceSite
position status: EQUAL
sequence position: 292

SequenceSite58
type: SequenceSite
position status: EQUAL
sequence position: 330

SequenceSite55
type: SequenceSite
position status: EQUAL
sequence position: 213

SequenceSite56
type: SequenceSite
position status: EQUAL
sequence position: 252

SequenceSite53
type: SequenceSite
position status: EQUAL
sequence position: 1521

SequenceSite54
type: SequenceSite
position status: EQUAL
sequence position: 175

SequenceSite51
type: SequenceSite
position status: EQUAL
sequence position: 321

SequenceSite52
type: SequenceSite
position status: EQUAL
sequence position: 1

SequenceSite50
type: SequenceSite
position status: EQUAL
sequence position: 206

Golgi membrane
type: CellularLocationVocabulary
xref: UnificationXref26

SRBP2_HUMAN
type: Protein
entity reference: SREBP2
data source: Reactome
name: SREBP2
name: Sterol regulatory element-binding protein 2
name: SREBF2(1-1141)
xref: UnificationXref134
xref: UnificationXref135
feature: FragmentFeature26
cellular location: Golgi membrane
comment: Reactome DB_ID: 1655747
display name: SREBF2

SCAP_CRIGR
type: Protein
entity reference: UniProt:P97260 SCAP
data source: Reactome
xref: UnificationXref137
xref: UnificationXref136
feature: FragmentFeature27
cellular location: Golgi membrane
comment: Reactome DB_ID: 1655750
display name: SCAP

UnificationXref9
type: UnificationXref
id: Q12772
db: UniProt

SC23A_MOUSE
type: Protein
entity reference: Sec23
data source: Reactome
name: Protein transport protein Sec23A
xref: UnificationXref128
xref: UnificationXref127
feature: FragmentFeature24
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1655767
display name: Sec23a

UnificationXref8
type: UnificationXref
id: 9606
db: NCBI Taxonomy

SAR1B_CRIGR
type: Protein
entity reference: SAR1
data source: Reactome
xref: UnificationXref130
xref: UnificationXref131
feature: FragmentFeature25
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1655715
display name: SAR1B

Pre-Notch2
type: Protein
entity reference: UniProt:O35516 Notch2
data source: Reactome
xref: UnificationXref153
xref: UnificationXref152
feature: FragmentFeature29
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1984029

Pre-Notch3
type: Protein
entity reference: Notch3
data source: Reactome
xref: UnificationXref155
xref: UnificationXref156
feature: FragmentFeature30
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1984032

Noth precursors
type: Protein
data source: Reactome
member physical entity: Pre-Notch3
member physical entity: Pre-Notch2
member physical entity: Pre-Notch4
member physical entity: Pre-Notch1
xref: UnificationXref161
xref: UnificationXref160
cellular location: endoplasmic reticulum membrane
comment: Converted from EntitySet in Reactome
comment: Reactome DB_ID: 1984033
display name: Pre-Notch

Pre-Notch1
type: Protein
entity reference: Notch1
data source: Reactome
xref: UnificationXref150
xref: UnificationXref149
feature: FragmentFeature28
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1984030

UnificationXref3
type: UnificationXref
id: P97260
db: UniProt

UnificationXref2
type: UnificationXref
id: 10029
db: NCBI Taxonomy

UnificationXref1
type: UnificationXref
id: GO:0005789
db: GENE ONTOLOGY

Pre-Notch4
type: Protein
entity reference: Notch4
data source: Reactome
xref: UnificationXref159
xref: UnificationXref158
feature: FragmentFeature31
cellular location: endoplasmic reticulum membrane
comment: Reactome DB_ID: 1984031

Fucosylated Notch precursors
type: Protein
data source: Reactome
member physical entity: Fucosylated Notch3 precursor
member physical entity: Fucosylated Notch4 precursor
member physical entity: Fucosylated Notch1 precursor
member physical entity: Fucosylated Notch2 precursor
xref: UnificationXref170
xref: UnificationXref171
cellular location: endoplasmic reticulum membrane
comment: Converted from EntitySet in Reactome
comment: Reactome DB_ID: 1986582
display name: Fuc-Pre-Notch

UnificationXref7
type: UnificationXref
id: REACT_148244
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148244.1

UnificationXref6
type: UnificationXref
id: 1655722
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655722

UnificationXref5
type: UnificationXref
id: REACT_148293
db: Reactome
id version: 1
comment: Reactome stable identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser_st_id?ST_ID=REACT_148293.1

UnificationXref4
type: UnificationXref
id: 1655763
db: Reactome Database ID Release 45
comment: Database identifier. Use this URL to connect to the web page of this instance in Reactome: http://www.reactome.org/cgi-bin/eventbrowser?DB=gk_current&ID=1655763

SequenceSite22
type: SequenceSite
position status: EQUAL
sequence position: 1155

SequenceSite23
type: SequenceSite
position status: EQUAL
sequence position: 1201

UniProt:G3HP78 Notch2
type: ProteinReference
name: I79_012584
name: Notch2
xref: UnificationXref33
organism: Cricetulus griseus

SequenceSite24
type: SequenceSite
position status: EQUAL
sequence position: 1320

SequenceSite25
type: SequenceSite
position status: EQUAL
sequence position: 1360

SequenceSite26
type: SequenceSite
position status: EQUAL
sequence position: 104

SequenceSite27
type: SequenceSite
position status: EQUAL
sequence position: 336

SequenceSite28
type: SequenceSite
position status: EQUAL
sequence position: 416

SequenceSite29
type: SequenceSite
position status: EQUAL
sequence position: 454

UDP
type: SmallMoleculeReference
name: uridine 5'-diphosphate
name: uridine 5'-diphosphate [ChEBI:17659]
xref: UnificationXref58

SequenceSite20
type: SequenceSite
position status: EQUAL
sequence position: 993

SequenceSite21
type: SequenceSite
position status: EQUAL
sequence position: 1117

SequenceSite19
type: SequenceSite
position status: EQUAL
sequence position: 955

SequenceSite35
type: SequenceSite
position status: EQUAL
sequence position: 755

SequenceSite36
type: SequenceSite
position status: EQUAL
sequence position: 985

SequenceSite33
type: SequenceSite
position status: EQUAL
sequence position: 680

SequenceSite34
type: SequenceSite
position status: EQUAL
sequence position: 717

SequenceSite39
type: SequenceSite
position status: EQUAL
sequence position: 1231

SequenceSite37
type: SequenceSite
position status: EQUAL
sequence position: 1023

SequenceSite38
type: SequenceSite
position status: EQUAL
sequence position: 1147

SequenceSite31
type: SequenceSite
position status: EQUAL
sequence position: 567

SequenceSite32
type: SequenceSite
position status: EQUAL
sequence position: 605

SequenceSite30
type: SequenceSite
position status: EQUAL
sequence position: 492

B4galt1
type: Protein
entity reference: Beta-1,4-galactosyltransferase 1
data source: Reactome
xref: UnificationXref62
xref: UnificationXref63
feature: FragmentFeature12
cellular location: Golgi membrane
comment: Reactome DB_ID: 2023200

Glc,Sia-Gal-GlcNAc-Fuc-Pre-Notch
type: Protein
data source: Reactome
member physical entity: Glc,Sia-Gal-GlcNAc-Fuc-Pre-Notch2
member physical entity: Glc,Sia-Gal-GlcNAc-Fuc-Pre-Notch4
member physical entity: Glc,Sia-Gal-GlcNAc-Fuc-Pre-Notch3
member physical entity: Glc,Sia-Gal-GlcNAc-Fuc-Pre-Notch1
xref: UnificationXref84
xref: UnificationXref83
cellular location: Golgi lumen
comment: Reactome DB_ID: 2032991
comment: Converted from EntitySet in Reactome

Glc,Gal-GlcNAc-Fuc-Pre-Notch3
type: Protein
entity reference: UniProt:G3HM56 Notch3
data source: Reactome
xref: UnificationXref53
xref: UnificationXref52
feature: ModificationFeature67
feature: ModificationFeature69
feature: ModificationFeature52
feature: ModificationFeature60
feature: ModificationFeature51
feature: ModificationFeature66
feature: ModificationFeature57
feature: ModificationFeature64
feature: ModificationFeature54
feature: FragmentFeature10
feature: ModificationFeature61
feature: ModificationFeature98
feature: ModificationFeature49
feature: ModificationFeature50
feature: ModificationFeature65
feature: ModificationFeature68
feature: ModificationFeature70
feature: ModificationFeature48
feature: ModificationFeature47
feature: ModificationFeature62
feature: ModificationFeature55
feature: ModificationFeature46
feature: ModificationFeature58
feature: ModificationFeature71
feature: ModificationFeature56
feature: ModificationFeature59
feature: ModificationFeature63
feature: ModificationFeature53
cellular location: Golgi membrane
comment: Reactome DB_ID: 2032274

Glc,Gal-GlcNAc-Fuc-Pre-Notch4
type: Protein
entity reference: Notch4
data source: Reactome
xref: UnificationXref54
xref: UnificationXref55
feature: ModificationFeature83
feature: ModificationFeature89
feature: ModificationFeature84
feature: ModificationFeature81
feature: ModificationFeature93
feature: ModificationFeature79
feature: ModificationFeature87
feature: ModificationFeature88
feature: ModificationFeature99
feature: ModificationFeature94
feature: ModificationFeature74
feature: ModificationFeature77
feature: ModificationFeature91
feature: ModificationFeature86
feature: ModificationFeature90
feature: ModificationFeature78
feature: ModificationFeature80
feature: ModificationFeature76
feature: ModificationFeature82
feature: ModificationFeature73
feature: ModificationFeature85
feature: ModificationFeature92
feature: ModificationFeature75
feature: FragmentFeature11
cellular location: Golgi membrane
comment: Reactome DB_ID: 2032275

Glc,Gal-GlcNAc-Fuc-Pre-Notch1
type: Protein
entity reference: UniProt:G3I6Z6 Notch1
data source: Reactome
xref: UnificationXref48
xref: UnificationXref49
feature: ModificationFeature29
feature: ModificationFeature32
feature: ModificationFeature9
feature: ModificationFeature20
feature: ModificationFeature33
feature: ModificationFeature21
feature: ModificationFeature16
feature: ModificationFeature31
feature: ModificationFeature26
feature: ModificationFeature24
feature: ModificationFeature8
feature: FragmentFeature8
feature: ModificationFeature25
feature: ModificationFeature96
feature: ModificationFeature4
feature: ModificationFeature17
feature: ModificationFeature19
feature: ModificationFeature2
feature: ModificationFeature6
feature: ModificationFeature10
feature: ModificationFeature5
feature: ModificationFeature30
feature: ModificationFeature15
feature: ModificationFeature13
feature: ModificationFeature3
feature: ModificationFeature22
feature: ModificationFeature14
feature: ModificationFeature7
feature: ModificationFeature11
feature: ModificationFeature23
feature: ModificationFeature27
feature: ModificationFeature28
feature: ModificationFeature12
feature: ModificationFeature1
feature: ModificationFeature18
cellular location: Golgi membrane
comment: Reactome DB_ID: 2032269

Glc,Gal-GlcNAc-Fuc-Pre-Notch2
type: Protein
entity reference: UniProt:G3HP78 Notch2
data source: Reactome
xref: UnificationXref50
xref: UnificationXref51
feature: ModificationFeature36
feature: ModificationFeature35
feature: ModificationFeature44
feature: ModificationFeature43
feature: ModificationFeature42
feature: ModificationFeature38
feature: ModificationFeature39
feature: FragmentFeature9
feature: ModificationFeature37
feature: ModificationFeature40
feature: ModificationFeature41
feature: ModificationFeature97
cellular location: Golgi membrane
comment: Reactome DB_ID: 2032271

Glc,Sia-Gal-GlcNAc-Fuc-Pre-Notch3
type: Protein
entity reference: UniProt:G3HM56 Notch3
data source: Reactome
xref: UnificationXref79
xref: UnificationXref80
feature: ModificationFeature67
feature: ModificationFeature102
feature: ModificationFeature69
feature: ModificationFeature52
feature: ModificationFeature60
feature: ModificationFeature51
feature: ModificationFeature66
feature: ModificationFeature57
feature: ModificationFeature64
feature: ModificationFeature54
feature: ModificationFeature61
feature: ModificationFeature49
feature: ModificationFeature50
feature: ModificationFeature65
feature: ModificationFeature68
feature: ModificationFeature70
feature: ModificationFeature48
feature: ModificationFeature47
feature: ModificationFeature62
feature: ModificationFeature55
feature: ModificationFeature46
feature: ModificationFeature58
feature: ModificationFeature71
feature: ModificationFeature56
feature: ModificationFeature59
feature: ModificationFeature63
feature: FragmentFeature15
feature: ModificationFeature53
cellular location: Golgi lumen
comment: Reactome DB_ID: 2032987

Glc,Sia-Gal-GlcNAc-Fuc-Pre-Notch4
type: Protein
entity reference: Notch4
data source: Reactome
xref: UnificationXref82
xref: UnificationXref81
feature: ModificationFeature83
feature: ModificationFeature89
feature: ModificationFeature84
feature: ModificationFeature81
feature: ModificationFeature93
feature: ModificationFeature79
feature: ModificationFeature87
feature: ModificationFeature88
feature: ModificationFeature94
feature: ModificationFeature74
feature: ModificationFeature77
feature: FragmentFeature16
feature: ModificationFeature91
feature: ModificationFeature86
feature: ModificationFeature90
feature: ModificationFeature78
feature: ModificationFeature80
feature: ModificationFeature76
feature: ModificationFeature82
feature: ModificationFeature103
feature: ModificationFeature73
feature: ModificationFeature85
feature: ModificationFeature92
feature: ModificationFeature75
cellular location: Golgi lumen
comment: Reactome DB_ID: 2032989

Glc,Sia-Gal-GlcNAc-Fuc-Pre-Notch1
type: Protein
entity reference: UniProt:G3I6Z6 Notch1
data source: Reactome
xref: UnificationXref75
xref: UnificationXref76
feature: ModificationFeature29
feature: ModificationFeature32
feature: ModificationFeature9
feature: ModificationFeature20
feature: ModificationFeature33
feature: ModificationFeature21
feature: ModificationFeature16
feature: ModificationFeature31
feature: ModificationFeature26
feature: ModificationFeature24
feature: ModificationFeature8
feature: ModificationFeature100
feature: ModificationFeature25
feature: ModificationFeature4
feature: ModificationFeature17
feature: ModificationFeature19
feature: ModificationFeature2
feature: ModificationFeature6
feature: ModificationFeature10
feature: ModificationFeature5
feature: ModificationFeature30
feature: ModificationFeature15
feature: FragmentFeature13
feature: ModificationFeature13
feature: ModificationFeature3
feature: ModificationFeature22
feature: ModificationFeature14
feature: ModificationFeature7
feature: ModificationFeature11
feature: ModificationFeature23
feature: ModificationFeature27
feature: ModificationFeature28
feature: ModificationFeature12
feature: ModificationFeature1
feature: ModificationFeature18
cellular location: Golgi lumen
comment: Reactome DB_ID: 2032984

Glc,Sia-Gal-GlcNAc-Fuc-Pre-Notch2
type: Protein
entity reference: UniProt:G3HP78 Notch2
data source: Reactome
xref: UnificationXref78
xref: UnificationXref77
feature: ModificationFeature101
feature: ModificationFeature36
feature: ModificationFeature35
feature: ModificationFeature44
feature: ModificationFeature43
feature: ModificationFeature42
feature: ModificationFeature38
feature: ModificationFeature39
feature: ModificationFeature37
feature: ModificationFeature40
feature: ModificationFeature41
feature: FragmentFeature14
cellular location: Golgi lumen
comment: Reactome DB_ID: 2032981