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notes: This model is taken from the Peer-reviewed publication>Husam M. Abu-Soud et al. Biochemistry 1999, 38, 12446-12451. This model shows kinetic binding of NMArginine to neuronal nitric oxide synthase. Model shows the substrate (NM-Arginine) binding to nNOS in a two-step reversible fashion. First there is rapid binding equilibrium between Im-nNOS and NM-Arginine to form an intermediate that contains bound imidazole and NM-arginine. This is followed by a slower conformational change in the Im-enzyme-substrate complex that is associated with release of bound imidazole and generation of a modified enzyme-substrate complex which is detected due to spectral change. Rates approximated based on data in Table 2. The rates for first reaction are not exact since only Kd known and appropriate graphs do not exist for matching the profile. This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2011 The BioModels.net Team. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not.. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.
substance units: 411908
is described by: http://identifiers.org/pubmed/10493814
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compartment: 020773
name: Abu-Soud1999_NMArginine
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reaction: 020933
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type: Reaction
kinetic law: 412018
product: 412005
reactant: 411993
reactant: 411981
notes: Binding of NM-Arginine to Imidazole bound rat brain neuronal Nitric Oxide Synthase. Only Kd is known, Kf and Kb need to be approximated accordingly to match kinetic profiles. Due to lack of appropriate graphs the choice of Kf and Kb here is arbitrary and matches only the Kd. Kd = 1.7mM = 1700uM Abu-Soud HM et al. Biochemistry. 1999 21;38(38):12446-51
fast: false
reversible: true

412018
type: KineticLaw
local parameters: 170740
local parameters: 170739
notes: kf*NM_minus_Arginine*Im_minus_nNOS-kb*Im_minus_nNOS_minus_NM_minus_Arginine

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notes: Release of Imidazole from complex of NM-Arginine and Imidazole bound rat brain neuronal Nitric Oxide Synthase. Abu-Soud HM et al. Biochemistry. 1999 21;38(38):12446-51
fast: false
reversible: true

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type: KineticLaw
local parameters: 170738
local parameters: 170737
notes: kf*Im_minus_nNOS_minus_NM_minus_Arginine-kb*nNOS_minus_NM_minus_Arginine*Imidazole

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