Activation of Ras by GAP (i.e NF1) Kf = 418 /sec Kb = 5.5 /sec Table 3, Phillips RA et al 2003 Biochemistry 3956-3965
First step in hydrolysis of GTP bound to Ras complexed with NF1 - a mammalian GAP Kf = 19.5 /sec Kb = 0.22 /sec Table 3, Phillips RA et al 2003 Biochemistry 3956-3965
Ras is an important regulator of cell growth in all eukaryotic cells. The model represent hydrolysis of active Ras-bound GTP to give inactive Ras-bound GDP catalyzed by GTPase activating proteins i.e NF1. The inactive Ras-bound GDP turns signalling off. This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2011 The BioModels.net Team. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not.. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.
Binding of NF1 to Ras.GTP NF1 is a mammalian GAP Kd = 5.3 uM Table 3, Phillips RA et al 2003 Biochemistry 3956-3965
Dissociation of NF1 from Ras.GDP NF1 is a mammalian GAP Kf = 46.5 /sec Kb = 1.2 /sec/uM Table 3, Phillips RA et al 2003 Biochemistry 3956-3965
Second step in hydrolysis of GTP bound to Ras is complexed with NF1 - a mammalian GAP Kf = 40 /sec Kb = 108 /M/sec = 1.08e-04 /uM/sec Phillips RA et al 2003 Biochemistry 3956-3965