MODEL4780784080
type: SBMLModel
species: 245849
substance units: 077912
species: 245907
reaction: 246087
species: 245807
units def: 077912
is described by: http://identifiers.org/pubmed/10400690
reaction: 246090
species: 245968
reaction: 246007
compartment: 245772
species: 245788
volume units: metaid_0000217
species: 245827
reaction: 246127
units def: 077863
compartment: 245787
species: 245947
reaction: 245988
reaction: 246147
length unit: length
name: Hayashi1999_NOSynth_Phospho
species: 245847
time units: 013121
species: 245867
area units: area
reaction: 246027
species: 245927
species: 245987
species: 245887
reaction: 246067
reaction: 246047
curated: false
species: 245908
units def: 077827
species: 245928
reaction: 246107
notes: This model features the phosphorylation of rat brain neuronal NOS expressed in E. coli or Sf9 cells, which leads to a decrease in Vmax of the phosphorylated enzyme, with little change of both the Km for L-arginine and Kact for CaM. This is based on Hayashi Y. et al. J Biol Chem. (1999) 274(29):20597-602. They report of phosphorylatin being carried out by CaM kinases I alpha, II alpha and IV. The rates used have been obtained from their paper and from other reported data. This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2011 The BioModels.net Team. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not.. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.

245849
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787

245787
type: Compartment
constant: true
size: 1.6667E-18
spatial dimensions: 3.0

077924
type: SBMLUnit
exponent: 1.0
scale: -6
multipler: 1.0
kind: MOLE

245907
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787

246087
type: Reaction
kinetic law: 078325
product: 078313
product: 078301
reactant: 078289
fast: false
reversible: false

078325
type: KineticLaw
local parameters: 078337
notes: k3*NOS_slash_CaMKIV_slash_kenz_slash_kenz_cplx

078337
type: LocalParameter
units: 077827
value: 3.0E-17

077851
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND

077839
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: LITRE

078313
type: SpeciesReference
stoichiometry: 1.0
species: 245928

245928
type: Species
notes: Activity is similar to CaMKIalpha, with ~0.7 mol of 32p/mol of nNOS. (Hayashi et 1999,JBC,274(29):20597-20602)
constant: true
boundary condition: true
has only substance units: false
initial concentration: 1.0
initial amount: NaN
in compartment: 245787

078301
type: SpeciesReference
stoichiometry: 1.0
species: 245807

245807
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787

078289
type: SpeciesReference
stoichiometry: 1.0
species: 245907

246090
type: Reaction
kinetic law: 078385
product: 078373
reactant: 078361
reactant: 078349
notes: Hayashi et al., 1999, JBC,274(29):20597-20602. and from various other datas.
fast: false
reversible: true

078385
type: KineticLaw
local parameters: 078409
local parameters: 078397
notes: k1*NOS_slash_nNOS*NOS_slash_CaMKIIalpha-k2*NOS_slash_CaMKIIalpha_slash_kenz_slash_kenz_cplx

078409
type: LocalParameter
units: 077827
value: 1.9E-16

078397
type: LocalParameter
units: 077863
value: 4.75E-17

077899
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND

077887
type: SBMLUnit
exponent: -1.0
scale: -6
multipler: 1.0
kind: MOLE

077875
type: SBMLUnit
exponent: 2.0
scale: 0
multipler: 1.0
kind: LITRE

078373
type: SpeciesReference
stoichiometry: 1.0
species: 245908

245908
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787

078361
type: SpeciesReference
stoichiometry: 1.0
species: 245947

245947
type: Species
notes: CaMKIIalpha caused the most rapid phosphorylation of nNOS, with half-maximal phosphorylation apparent at 3 min and plateau level at 10 min. (Hayashi et al., 1999,JBC,274(29):20597-20602). The Maximal Phosphorylation of nNOS was observed at ~0.4 mol of 32p/mol of nNOS under their conditions.
constant: true
boundary condition: true
has only substance units: false
initial concentration: 1.0
initial amount: NaN
in compartment: 245787

078349
type: SpeciesReference
stoichiometry: 1.0
species: 245788

245788
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.5
initial amount: NaN
in compartment: 245787

245968
type: Species
notes: Phosphorylates nNOS, but not as effective as CaMKIIalpha. (Hayashi et al., 1999,JBC,274(29):20597-20602.) Report of Plateau Level in their phosphorylation plots reaching after 100 min. -- ~0.4 mol of 32p/mol of nNOS.
constant: true
boundary condition: true
has only substance units: false
initial concentration: 1.0
initial amount: NaN
in compartment: 245787

246007
type: Reaction
kinetic law: 078034
product: 078022
reactant: 078010
notes: kf -13.9 These rates used to keep the basal level of nNOS at reasonable levels.
fast: false
reversible: true

078034
type: KineticLaw
local parameters: 078058
local parameters: 078046
notes: kf*NOS_slash_NOS_star_-kb*NOS_slash_nNOS

078058
type: LocalParameter
units: 077827
value: 0.0

078046
type: LocalParameter
units: 077827
value: 2.31666666666667E-17

078022
type: SpeciesReference
stoichiometry: 1.0
species: 245788

078010
type: SpeciesReference
stoichiometry: 1.0
species: 245807

245772
type: Compartment
constant: true
size: 1.0E-18
spatial dimensions: 3.0

245827
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787

246127
type: Reaction
kinetic law: 078517
product: 078505
reactant: 078493
reactant: 078481
notes: enzyme parameters used from different literature. Hayashi et al., JBC,274(29):20597-20602.
fast: false
reversible: true

078517
type: KineticLaw
local parameters: 078541
local parameters: 078529
notes: k1*NOS_slash_nNOS*NOS_slash_CaMKIalpha-k2*NOS_slash_CaMKIalpha_slash_kenz_slash_kenz_cplx

078541
type: LocalParameter
units: 077827
value: 1.13333333333333E-16

078529
type: LocalParameter
units: 077863
value: 2.83333333333333E-17

078505
type: SpeciesReference
stoichiometry: 1.0
species: 245927

245927
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787

078493
type: SpeciesReference
stoichiometry: 1.0
species: 245968

078481
type: SpeciesReference
stoichiometry: 1.0
species: 245788

245988
type: Reaction
kinetic law: 077974
product: 077961
reactant: 077949
reactant: 077936
notes: Those binding CaM have a high Kd, including nNOS, ~<=10nM. The binding of CaM to nNOS has been demonstrated to act as the trigger necessary for electron transfer and catalytic activity. (Marletta, 1997;36:12337-12345).
fast: false
reversible: true

077974
type: KineticLaw
local parameters: 077998
local parameters: 077986
notes: kf*NOS_slash_nNOS*NOS_slash_CaM_minus_Ca4-kb*NOS_slash_Ca_minus_CaMnNOS

077998
type: LocalParameter
units: 077827
value: 8.33333333333333E-20

077986
type: LocalParameter
units: 077863
value: 5.41666666666667E-18

077961
type: SpeciesReference
stoichiometry: 1.0
species: 245827

077949
type: SpeciesReference
stoichiometry: 1.0
species: 245987

245987
type: Species
constant: true
boundary condition: true
has only substance units: false
initial concentration: 20.0
initial amount: NaN
in compartment: 245787

077936
type: SpeciesReference
stoichiometry: 1.0
species: 245788

246147
type: Reaction
kinetic law: 078589
product: 078577
product: 078565
reactant: 078553
fast: false
reversible: false

078589
type: KineticLaw
local parameters: 078601
notes: k3*NOS_slash_CaMKIalpha_slash_kenz_slash_kenz_cplx

078601
type: LocalParameter
units: 077827
value: 2.83333333333333E-17

078577
type: SpeciesReference
stoichiometry: 1.0
species: 245968

078565
type: SpeciesReference
stoichiometry: 1.0
species: 245807

078553
type: SpeciesReference
stoichiometry: 1.0
species: 245927

245847
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787

245867
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787

246027
type: Reaction
kinetic law: 078107
product: 078094
reactant: 078082
reactant: 078070
notes: Km for purified NOS is estimated between 1 - 10 uM. (Prog in Neurobiology, 2001, 64: 365-391) Vmax for unphosporylated NOS, the active form, is 500-1500 nmol/nmol/min (Montellano et al., 1998, JBC,26(12): 1185-1189). Hayashi et al., JBC, 1999, 274(29):20597-20602 report Vmax (nmol/min/mg) of nNOS Unphosporylated at 95.7 (+-) 4.2
fast: false
reversible: true

078107
type: KineticLaw
local parameters: 078132
local parameters: 078120
notes: k1*NOS_slash_Larg*NOS_slash_Ca_minus_CaMnNOS-k2*NOS_slash_Ca_minus_CaMnNOS_slash_kenz_slash_kenz_cplx

078132
type: LocalParameter
units: 077827
value: 1.11113333333333E-16

078120
type: LocalParameter
units: 077863
value: 1.38891666666667E-17

078094
type: SpeciesReference
stoichiometry: 1.0
species: 245847

078082
type: SpeciesReference
stoichiometry: 1.0
species: 245827

078070
type: SpeciesReference
stoichiometry: 1.0
species: 245887

245887
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 100.0
initial amount: NaN
in compartment: 245787

246067
type: Reaction
kinetic law: 078253
product: 078241
reactant: 078229
reactant: 078217
notes: Hayashi et al., 1999, JBC,274(29):20597-20602. and other reported data from sources.
fast: false
reversible: true

078253
type: KineticLaw
local parameters: 078277
local parameters: 078265
notes: k1*NOS_slash_nNOS*NOS_slash_CaMKIV-k2*NOS_slash_CaMKIV_slash_kenz_slash_kenz_cplx

078277
type: LocalParameter
units: 077827
value: 1.2E-16

078265
type: LocalParameter
units: 077863
value: 3.0E-17

078241
type: SpeciesReference
stoichiometry: 1.0
species: 245907

078229
type: SpeciesReference
stoichiometry: 1.0
species: 245928

078217
type: SpeciesReference
stoichiometry: 1.0
species: 245788

246047
type: Reaction
kinetic law: 078193
product: 078181
product: 078169
product: 078156
reactant: 078144
fast: false
reversible: false

078193
type: KineticLaw
local parameters: 078205
notes: k3*NOS_slash_Ca_minus_CaMnNOS_slash_kenz_slash_kenz_cplx

078205
type: LocalParameter
units: 077827
value: 2.77783333333333E-17

078181
type: SpeciesReference
stoichiometry: 1.0
species: 245827

078169
type: SpeciesReference
stoichiometry: 1.0
species: 245867

078156
type: SpeciesReference
stoichiometry: 1.0
species: 245849

078144
type: SpeciesReference
stoichiometry: 1.0
species: 245847

246107
type: Reaction
kinetic law: 078457
product: 078445
product: 078433
reactant: 078421
fast: false
reversible: false

078457
type: KineticLaw
local parameters: 078469
notes: k3*NOS_slash_CaMKIIalpha_slash_kenz_slash_kenz_cplx

078469
type: LocalParameter
units: 077827
value: 4.75E-17

078445
type: SpeciesReference
stoichiometry: 1.0
species: 245947

078433
type: SpeciesReference
stoichiometry: 1.0
species: 245807

078421
type: SpeciesReference
stoichiometry: 1.0
species: 245908