This is a modification by Condorelli et al., 2001, Biophys J of the recent binding scheme of Zhao et al., to include the NO dissociation from fully active complex of sGC... The dissociation is characterised by the respective first order rate constants depicted in the model... Rates are obtained at 4 degree C...
Rates used directly from Condorelli et al., 2001, Biophysical J, 80:2110-2119 .... Rates originally from Zhao et al., 1999, PNAS, 96:14753-14758 from stopped flow spectroscopy experiments performed 4 degree C.
Rates at 4 degree C... used directly from paper by Condorelli P, 2001, Biophysical J, 80:2110-2119, which in turn have been obtained mainly from Zhao et al., PNAS,96:14753-14758.
This model features the observations of Condorelli P, George SC. Biophys J. (2001) 80(5):2110-9. They propose a in vivo mechanism for the binding of free NO to sGC based on in vitro data within both pulmonary and vascular smooth muscle. All the parameters used have been obtained from reported results based on experiments. One important conclusion they make is that control of sGC in vivo is most likely to be ultra-sensitive and that activation in vivo occurs at lower NO concentrations than reported. This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2011 The BioModels.net Team. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not.. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.
Rates from stopped flow experiments performed at 4 degree C from Zhao et al., 1999, PNAS,96:14753-14758. Same rates used by Condorelli P, for simulations...
Km values for the basal and fully activated forms of sGC have been determined as 85-120 uM and 58 uM, respectively ... (Ignarro et al., 1982, BBA, 718:49-59)..and the same has been used by Condorelli et al., 2001... Vmax values are around 10-100 nmol/min/mg (without NO) and 10-40 umol/min/mg (with NO) ..... thru personal correspondence with T Bellamy,