Km - 2 Vmax - 3.87 Thru personal Correspondence with Shinya Kuroda... rates initially from Turko et al., 1998, Biochem J, 329, 505-510
This model of sGC is based on the paper byKuroda S. et al. J Neurosci. (2001) 21(15):5693-702 . This models features the activation of sGC by NO, synthesis of cGMP, activity of PKG and PP2A in the synapse. The rates and concentrations have been taken from literature. It contains only the part described in figure 1B of the article. This model originates from BioModels Database: A Database of Annotated Published Models. It is copyright (c) 2005-2011 The BioModels.net Team. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not.. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.
Guanylyl Cyclase ... Intracellular concentration is around 3 uM, as sGC is enrcihed in the Purkinje cells (Shinya Kuroda, personal corresspondence).. Conc assumed on the basis of reported data in Ariano et al., PNAS,79:297-300.
Kd 250 nM (Stone and Marletta, 1996).. kf - 0.01 kb - 0.0025 Shinya Kuroda, J.Neurosci, 2001, ...
Kd - 0.27 uM Shinya Kuroda, J.Neurosci, 21(15):5693-5702.
kf - 0.0001/sec Shinya Kuroda, J.Neurosci, 2001, 21(15):5693-5702 ... and thru personal ...
degradation of NO ... kf - 0.673 / sec .... Shinya Kuroda, J.Neurosci, 2001, 21(15):5693-5702