MODEL1202090002
type: SBMLModel
reaction: re23
parameter: inactive Tem1 Cytosol
reaction: re35
units def: time
units def: pmps
reaction: re39
compartment: default
reaction: re14
parameter: total Bfa1 Cytosol
parameter: konB4
parameter: total Tem1 SPB
units def: molecules
reaction: re18
species: s19
reaction: re58
rule: CDMT00190
species: s8
species: s4
time units: time
rule: CDMT00189
reaction: re7
units def: substance
reaction: re12
parameter: koffB
reaction: re16
species: s62
reaction: re22
reaction: re4
reaction: re56
species: s37
rule: CDMT00194
parameter: kfCdc5
reaction: re61
reaction: re9
compartment: c2
parameter: alpha
is described by: msb.2012.15
units def: ps
parameter: inactive Bfa1 SPB
reaction: re24
reaction: re54
reaction: re28
reaction: re66
species: s53
parameter: krCdc5
units def: area
parameter: koffT
substance units: substance
parameter: inactive Tem1 SPB
rule: CDMT00187
units def: length
units def: volume
reaction: re52
parameter: knex
species: s9
reaction: re8
species: s16
reaction: re13
rule: CDMT00184
species: s2
species: s1
reaction: re57
notes: This model is from the article: A dynamical model of the spindle position checkpoint Ayse Koca Caydasi, Maiko Lohel, Gerd Grünert, Peter Dittrich, Gislene Pereira & Bashar Ibrahim Molecular Systems Biology 2012; 582 PMEDID , doi: 10.1038/msb.2012.15 Abstract: The orientation of the mitotic spindle with respect to the polarity axis is crucial for the accuracy of asymmetric cell division. In budding yeast, a surveillance mechanism called the spindle position checkpoint (SPOC) prevents exit from mitosis when the mitotic spindle fails to align along the mother-to-daughter polarity axis. SPOC arrest relies upon inhibition of the GTPase Tem1 by the GTPase-activating protein (GAP) complex Bfa1–Bub2. Importantly, reactions signaling mitotic exit take place at yeast centrosomes (named spindle pole bodies, SPBs) and the GAP complex also promotes SPB localization of Tem1. Yet, whether the regulation of Tem1 by Bfa1–Bub2 takes place only at the SPBs remains elusive. Here, we present a quantitative analysis of Bfa1–Bub2 and Tem1 localization at the SPBs. Based on the measured SPB-bound protein levels, we introduce a dynamical model of the SPOC that describes the regulation of Bfa1 and Tem1. Our model suggests that Bfa1 interacts with Tem1 in the cytoplasm as well as at the SPBs to provide efficient Tem1 inhibition. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanc…
area units: area
parameter: konB5T
rule: CDMT00191
parameter: active Tem1 SPB
parameter: inactive Bfa1 Cytosol
species: s7
reaction: re11
event: spindle alignment
reaction: re15
reaction: re55
reaction: re21
parameter: NA
parameter: khydBT
reaction: re67
parameter: konT
reaction: re34

re23
type: Reaction
kinetic law: CDMT00148
product: CDMT00054
reactant: CDMT00053
fast: false
reversible: false
name: R29: Phosphorylation of SPB-bound Bfa1:Tem1GDP by Cdc5

CDMT00054
type: SpeciesReference
stoichiometry: 1.0
species: s45

s45
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1P5-Tem1GDP

substance
type: SBMLUnitsDef
unit: CDMT00005
name: substance

CDMT00005
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: MOLE

c3
type: Compartment
constant: true
units: litre
size: 3.0E-18
spatial dimensions: 3.0
name: SPB

CDMT00053
type: SpeciesReference
stoichiometry: 1.0
species: s37

s37
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1-Tem1GDP

inactive Tem1 Cytosol
type: Parameter
constant: false
units: molecules
value: 0.0
name: Inactive Tem1 in the cytosol

molecules
type: SBMLUnitsDef
unit: CDMT00001
name: molecules

CDMT00001
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: DIMENSIONLESS

re35
type: Reaction
kinetic law: CDMT00156
product: CDMT00082
reactant: CDMT00081
reactant: CDMT00080
fast: false
reversible: true
name: R13: Rev. association of Tem1GDP with SPB-bound Bfa1

CDMT00082
type: SpeciesReference
stoichiometry: 1.0
species: s37

CDMT00081
type: SpeciesReference
stoichiometry: 1.0
species: s10

s10
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 8.33E-5
initial amount: NaN
in compartment: c3
name: B-Bfa1

CDMT00080
type: SpeciesReference
stoichiometry: 1.0
species: s9

s9
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 7.99E-9
initial amount: NaN
in compartment: c2
name: Tem1GDP

c2
type: Compartment
constant: true
units: litre
size: 1.0E-13
spatial dimensions: 3.0
name: Cytosol

time
type: SBMLUnitsDef
unit: CDMT00015
name: time

CDMT00015
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: SECOND

pmps
type: SBMLUnitsDef
unit: CDMT00130
unit: CDMT00129
unit: CDMT00128
name: per molar per second

CDMT00130
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND

CDMT00129
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: LITRE

CDMT00128
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: MOLE

re39
type: Reaction
kinetic law: CDMT00159
product: CDMT00089
reactant: CDMT00088
fast: false
reversible: false
name: R46: GAP-accelerated GTP-hydrolysis

CDMT00089
type: SpeciesReference
stoichiometry: 1.0
species: s37

CDMT00088
type: SpeciesReference
stoichiometry: 1.0
species: s25

s25
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1-Tem1GTP

default
type: Compartment
constant: true
units: litre
size: 1.0
spatial dimensions: 3.0

re14
type: Reaction
kinetic law: CDMT00141
product: CDMT00035
reactant: CDMT00034
reactant: CDMT00033
fast: false
reversible: true
name: R17: Rev. association of Tem1GTP with Kin4-phosphorylated Bfa1

CDMT00035
type: SpeciesReference
stoichiometry: 1.0
species: s53

s53
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1P4-Tem1GTP

CDMT00034
type: SpeciesReference
stoichiometry: 1.0
species: s8

s8
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 4.91E-8
initial amount: NaN
in compartment: c2
name: Tem1GTP

CDMT00033
type: SpeciesReference
stoichiometry: 1.0
species: s4

s4
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1P4

total Bfa1 Cytosol
type: Parameter
constant: false
units: molecules
value: 0.0
name: Total Bfa1 in the Cytosol

konB4
type: Parameter
constant: true
units: pmps
value: 20000.0

total Tem1 SPB
type: Parameter
constant: false
units: molecules
value: 0.0
name: Total Tem1 at the SPB

re18
type: Reaction
kinetic law: CDMT00144
product: CDMT00044
reactant: CDMT00043
reactant: CDMT00042
fast: false
reversible: true
name: R11: Rev. association of Tem1GTP with SPB-bound Kin4-phosphorylated Bfa1

CDMT00044
type: SpeciesReference
stoichiometry: 1.0
species: s29

s29
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1P4-Tem1GTP

CDMT00043
type: SpeciesReference
stoichiometry: 1.0
species: s13

s13
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1P4

CDMT00042
type: SpeciesReference
stoichiometry: 1.0
species: s8

s19
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: T-Tem1GTP

re58
type: Reaction
kinetic law: CDMT00172
product: CDMT00127
reactant: CDMT00126
fast: false
reversible: false
name: R45: GTP-hydrolysis with intrinisc GTPase activity

CDMT00127
type: SpeciesReference
stoichiometry: 1.0
species: s65

s65
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1P5-Tem1GDP

CDMT00126
type: SpeciesReference
stoichiometry: 1.0
species: s56

s56
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1P5-Tem1GTP

re7
type: Reaction
kinetic law: CDMT00134
product: CDMT00017
reactant: CDMT00016
fast: false
reversible: false
name: R24: Phosphorylation of SPB-bound Bfa1 by Kin4

CDMT00017
type: SpeciesReference
stoichiometry: 1.0
species: s13

CDMT00016
type: SpeciesReference
stoichiometry: 1.0
species: s10

re12
type: Reaction
kinetic law: CDMT00139
product: CDMT00030
reactant: CDMT00029
fast: false
reversible: false
name: R25: Phosphorylation of SPB-bound Bfa1:Tem1GTP by Kin4

CDMT00030
type: SpeciesReference
stoichiometry: 1.0
species: s29

CDMT00029
type: SpeciesReference
stoichiometry: 1.0
species: s25

koffB
type: Parameter
constant: true
units: ps
value: 0.0012

ps
type: SBMLUnitsDef
unit: CDMT00131
name: per second

CDMT00131
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND

re16
type: Reaction
kinetic law: CDMT00143
product: CDMT00041
reactant: CDMT00040
reactant: CDMT00039
fast: false
reversible: true
name: R18: Rev. association of Tem1GTP with Cdc5-phosphorylated Bfa1

CDMT00041
type: SpeciesReference
stoichiometry: 1.0
species: s56

CDMT00040
type: SpeciesReference
stoichiometry: 1.0
species: s8

CDMT00039
type: SpeciesReference
stoichiometry: 1.0
species: s7

s7
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1P5

s62
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1P4-Tem1GDP

re22
type: Reaction
kinetic law: CDMT00147
product: CDMT00052
reactant: CDMT00051
fast: false
reversible: false
name: R26: Phosphorylation of SPB-bound Bfa1:Tem1GDP by Kin4

CDMT00052
type: SpeciesReference
stoichiometry: 1.0
species: s41

s41
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1P4-Tem1GDP

CDMT00051
type: SpeciesReference
stoichiometry: 1.0
species: s37

re4
type: Reaction
kinetic law: CDMT00132
product: CDMT00009
reactant: CDMT00008
reactant: CDMT00007
fast: false
reversible: true
name: R2: Rev. SPB-association of Kin4-phosphorylated Bfa1

CDMT00009
type: SpeciesReference
stoichiometry: 1.0
species: s13

CDMT00008
type: SpeciesReference
stoichiometry: 1.0
species: s1

s1
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 8.33E-5
initial amount: NaN
in compartment: c3
name: B

CDMT00007
type: SpeciesReference
stoichiometry: 1.0
species: s4

re56
type: Reaction
kinetic law: CDMT00170
product: CDMT00123
reactant: CDMT00122
fast: false
reversible: false
name: R44: GAP-accelerated GTP-hydrolysis

CDMT00123
type: SpeciesReference
stoichiometry: 1.0
species: s62

CDMT00122
type: SpeciesReference
stoichiometry: 1.0
species: s53

kfCdc5
type: Parameter
constant: true
units: ps
value: 1.0

re61
type: Reaction
kinetic law: CDMT00175
product: CDMT00076
reactant: CDMT00075
fast: false
reversible: false
name: R37: Dephosphorylation of Cdc5-phosphorylated Bfa1 presumably by PP2A

CDMT00076
type: SpeciesReference
stoichiometry: 1.0
species: s50

s50
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1-Tem1GTP

CDMT00075
type: SpeciesReference
stoichiometry: 1.0
species: s56

re9
type: Reaction
kinetic law: CDMT00136
product: CDMT00022
reactant: CDMT00021
reactant: CDMT00020
fast: false
reversible: true
name: R5: Rev. SPB-association of Kin4-phosphorylated Bfa1-Tem1GTP complex

CDMT00022
type: SpeciesReference
stoichiometry: 1.0
species: s29

CDMT00021
type: SpeciesReference
stoichiometry: 1.0
species: s1

CDMT00020
type: SpeciesReference
stoichiometry: 1.0
species: s53

alpha
type: Parameter
constant: true
units: dimensionless
value: 1.0
name: alpha

inactive Bfa1 SPB
type: Parameter
constant: false
units: molecules
value: 0.0
name: Inactive Bfa1 at the SPB

re24
type: Reaction
kinetic law: CDMT00149
product: CDMT00057
reactant: CDMT00056
reactant: CDMT00055
fast: false
reversible: true
name: R8: Rev. SPB-association of Kin4 phosphorylated Bfa1-Tem1GDP complex

CDMT00057
type: SpeciesReference
stoichiometry: 1.0
species: s41

CDMT00056
type: SpeciesReference
stoichiometry: 1.0
species: s1

CDMT00055
type: SpeciesReference
stoichiometry: 1.0
species: s62

re54
type: Reaction
kinetic law: CDMT00168
product: CDMT00113
reactant: CDMT00112
fast: false
reversible: false
name: R42: GDP- for GTP nucleotide exchange

CDMT00113
type: SpeciesReference
stoichiometry: 1.0
species: s19

CDMT00112
type: SpeciesReference
stoichiometry: 1.0
species: s22

s22
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: T-Tem1GDP

re28
type: Reaction
kinetic law: CDMT00152
product: CDMT00066
reactant: CDMT00065
reactant: CDMT00064
fast: false
reversible: true
name: R20: Rev. association of Tem1GDP with Kin4-phosphorylated Bfa1

CDMT00066
type: SpeciesReference
stoichiometry: 1.0
species: s62

CDMT00065
type: SpeciesReference
stoichiometry: 1.0
species: s9

CDMT00064
type: SpeciesReference
stoichiometry: 1.0
species: s4

re66
type: Reaction
kinetic law: CDMT00178
product: CDMT00117
reactant: CDMT00116
fast: false
reversible: false
name: R38: Dephosphorylation of Cdc5-phosphorylated Bfa1 presumably by PP2A

CDMT00117
type: SpeciesReference
stoichiometry: 1.0
species: s59

s59
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1-Tem1GDP

CDMT00116
type: SpeciesReference
stoichiometry: 1.0
species: s65

krCdc5
type: Parameter
constant: true
units: ps
value: 0.01

area
type: SBMLUnitsDef
unit: CDMT00013
name: area

CDMT00013
type: SBMLUnit
exponent: 2.0
scale: 0
multipler: 1.0
kind: METRE

koffT
type: Parameter
constant: true
units: ps
value: 0.183

inactive Tem1 SPB
type: Parameter
constant: false
units: molecules
value: 0.0
name: Inactive Tem1 at the SPB

length
type: SBMLUnitsDef
unit: CDMT00014
name: length

CDMT00014
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: METRE

volume
type: SBMLUnitsDef
unit: CDMT00006
name: volume

CDMT00006
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: LITRE

re52
type: Reaction
kinetic law: CDMT00166
product: CDMT00109
reactant: CDMT00108
fast: false
reversible: false
name: R40: GDP- for GTP nucleotide exchange

CDMT00109
type: SpeciesReference
stoichiometry: 1.0
species: s8

CDMT00108
type: SpeciesReference
stoichiometry: 1.0
species: s9

knex
type: Parameter
constant: true
units: ps
value: 0.0136

re8
type: Reaction
kinetic law: CDMT00135
product: CDMT00019
reactant: CDMT00018
fast: false
reversible: false
name: R27: Phosphorylation of SPB-bound Bfa1 by Cdc5

CDMT00019
type: SpeciesReference
stoichiometry: 1.0
species: s16

s16
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1P5

CDMT00018
type: SpeciesReference
stoichiometry: 1.0
species: s10

re13
type: Reaction
kinetic law: CDMT00140
product: CDMT00032
reactant: CDMT00031
fast: false
reversible: false
name: R28: Phosphorylation of SPB-bound Bfa1:Tem1GTP by Cdc5

CDMT00032
type: SpeciesReference
stoichiometry: 1.0
species: s33

s33
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1P5-Tem1GTP

CDMT00031
type: SpeciesReference
stoichiometry: 1.0
species: s25

s2
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 1.66E-4
initial amount: NaN
in compartment: c3
name: T

re57
type: Reaction
kinetic law: CDMT00171
product: CDMT00125
reactant: CDMT00124
fast: false
reversible: false
name: R43: GAP-accelerated GTP-hydrolysis

CDMT00125
type: SpeciesReference
stoichiometry: 1.0
species: s59

CDMT00124
type: SpeciesReference
stoichiometry: 1.0
species: s50

konB5T
type: Parameter
constant: true
units: pmps
value: 7000000.0

active Tem1 SPB
type: Parameter
constant: false
units: molecules
value: 0.0
name: Active Tem1 at the SPB

inactive Bfa1 Cytosol
type: Parameter
constant: false
units: molecules
value: 0.0
name: Inactive Bfa1 in the cytosol

re11
type: Reaction
kinetic law: CDMT00138
product: CDMT00028
reactant: CDMT00027
reactant: CDMT00026
fast: false
reversible: true
name: R6: Rev. SPB-association of Cdc5-phosphorylated Bfa1-Tem1GTP complex

CDMT00028
type: SpeciesReference
stoichiometry: 1.0
species: s33

CDMT00027
type: SpeciesReference
stoichiometry: 1.0
species: s1

CDMT00026
type: SpeciesReference
stoichiometry: 1.0
species: s56

spindle alignment
type: Event
event assignment: CDMT00182
trigger: CDMT00181
use values from trigger time: true
name: SPOC deactivation upon correct spindle alignment

http://identifiers.org/biomodels.db/MODEL1202090002#u
type: Parameter
constant: false
units: dimensionless
value: 1.0

CDMT00181
type: Trigger
persistant: true
initial value: true

re15
type: Reaction
kinetic law: CDMT00142
product: CDMT00038
reactant: CDMT00037
reactant: CDMT00036
fast: false
reversible: true
name: R16: Rev. association of Tem1GTP with Bfa1

CDMT00038
type: SpeciesReference
stoichiometry: 1.0
species: s50

CDMT00037
type: SpeciesReference
stoichiometry: 1.0
species: s8

CDMT00036
type: SpeciesReference
stoichiometry: 1.0
species: s3

s3
type: Species
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 2.03E-8
initial amount: NaN
in compartment: c2
name: Bfa1

re55
type: Reaction
kinetic law: CDMT00169
product: CDMT00115
reactant: CDMT00114
fast: false
reversible: false
name: R41: GTP-hydrolysis with intrinisc GTPase activity

CDMT00115
type: SpeciesReference
stoichiometry: 1.0
species: s22

CDMT00114
type: SpeciesReference
stoichiometry: 1.0
species: s19

re21
type: Reaction
kinetic law: CDMT00146
product: CDMT00050
reactant: CDMT00049
reactant: CDMT00048
fast: false
reversible: true
name: R12: Rev. association of Tem1GTP with SPB-bound Cdc5-phosphorylated Bfa1

CDMT00050
type: SpeciesReference
stoichiometry: 1.0
species: s33

CDMT00049
type: SpeciesReference
stoichiometry: 1.0
species: s16

CDMT00048
type: SpeciesReference
stoichiometry: 1.0
species: s8

NA
type: Parameter
constant: true
units: pmole
value: 6.022E23
name: Avogadro's number

pmole
type: SBMLUnitsDef
unit: CDMT00180
name: per mole

CDMT00180
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: MOLE

khydBT
type: Parameter
constant: true
units: ps
value: 2.0

re67
type: Reaction
kinetic law: CDMT00179
product: CDMT00119
reactant: CDMT00118
fast: false
reversible: false
name: R32: Phosphorylation of Bfa1:Tem1GDP by cytosolic Kin4

CDMT00119
type: SpeciesReference
stoichiometry: 1.0
species: s62

CDMT00118
type: SpeciesReference
stoichiometry: 1.0
species: s59

konT
type: Parameter
constant: true
units: pmps
value: 1900000.0

re34
type: Reaction
kinetic law: CDMT00155
product: CDMT00079
reactant: CDMT00078
reactant: CDMT00077
fast: false
reversible: true
name: R14: Rev. association of Tem1GDP with SPB-bound Kin4-phosphorylated Bfa1

CDMT00079
type: SpeciesReference
stoichiometry: 1.0
species: s41

CDMT00078
type: SpeciesReference
stoichiometry: 1.0
species: s13

CDMT00077
type: SpeciesReference
stoichiometry: 1.0
species: s9

re26
type: Reaction
kinetic law: CDMT00151
product: CDMT00063
reactant: CDMT00062
reactant: CDMT00061
fast: false
reversible: true
name: R9: Rev. SPB-association of Cdc5-phosphorylated Bfa1-Tem1GDP complex

CDMT00063
type: SpeciesReference
stoichiometry: 1.0
species: s45

CDMT00062
type: SpeciesReference
stoichiometry: 1.0
species: s1

CDMT00061
type: SpeciesReference
stoichiometry: 1.0
species: s65

kfKin4
type: Parameter
constant: true
units: ps
value: 1000.0

re60
type: Reaction
kinetic law: CDMT00174
product: CDMT00074
reactant: CDMT00073
fast: false
reversible: false
name: R34: Dephosphorylation of Bfa1 by a yet unidentified phosphatase counteracting Kin4

CDMT00074
type: SpeciesReference
stoichiometry: 1.0
species: s50

CDMT00073
type: SpeciesReference
stoichiometry: 1.0
species: s53

konBT
type: Parameter
constant: true
units: pmps
value: 3.65E7

koffBT
type: Parameter
constant: true
units: ps
value: 0.183

W3 CDTF: Thu May 10 14:50:01 BST 2012

re53
type: Reaction
kinetic law: CDMT00167
product: CDMT00111
reactant: CDMT00110
fast: false
reversible: false
name: R39: GTP-hydrolysis with intrinisc GTPase activity

CDMT00111
type: SpeciesReference
stoichiometry: 1.0
species: s9

CDMT00110
type: SpeciesReference
stoichiometry: 1.0
species: s8

re65
type: Reaction
kinetic law: CDMT00177
product: CDMT00101
reactant: CDMT00100
fast: false
reversible: false
name: R35: Dephosphorylation of Bfa1 by a yet unidentified phosphatase counteracting Kin4

CDMT00101
type: SpeciesReference
stoichiometry: 1.0
species: s59

CDMT00100
type: SpeciesReference
stoichiometry: 1.0
species: s62

khydB4T
type: Parameter
constant: true
units: ps
value: 2.0

re10
type: Reaction
kinetic law: CDMT00137
product: CDMT00025
reactant: CDMT00024
reactant: CDMT00023
fast: false
reversible: true
name: R4: Rev. SPB-association of Bfa1-Tem1GTP complex

CDMT00025
type: SpeciesReference
stoichiometry: 1.0
species: s25

CDMT00024
type: SpeciesReference
stoichiometry: 1.0
species: s1

CDMT00023
type: SpeciesReference
stoichiometry: 1.0
species: s50

re36
type: Reaction
kinetic law: CDMT00157
product: CDMT00085
reactant: CDMT00084
reactant: CDMT00083
fast: false
reversible: true
name: R15: Rev. association of Tem1GDP with SPB-bound Cdc5-phosphorylated Bfa1

CDMT00085
type: SpeciesReference
stoichiometry: 1.0
species: s45

CDMT00084
type: SpeciesReference
stoichiometry: 1.0
species: s16

CDMT00083
type: SpeciesReference
stoichiometry: 1.0
species: s9

re46
type: Reaction
kinetic law: CDMT00162
product: CDMT00097
reactant: CDMT00096
reactant: CDMT00095
fast: false
reversible: true
name: R22: Rev. SPB-association of Tem1GTP

CDMT00097
type: SpeciesReference
stoichiometry: 1.0
species: s19

CDMT00096
type: SpeciesReference
stoichiometry: 1.0
species: s2

CDMT00095
type: SpeciesReference
stoichiometry: 1.0
species: s8

re51
type: Reaction
kinetic law: CDMT00165
product: CDMT00107
reactant: CDMT00106
fast: false
reversible: false
name: R36: Dephosphorylation of Cdc5-phosphorylated Bfa1 presumably by PP2A

CDMT00107
type: SpeciesReference
stoichiometry: 1.0
species: s3

CDMT00106
type: SpeciesReference
stoichiometry: 1.0
species: s7

re63
type: Reaction
kinetic law: CDMT00176
product: CDMT00099
reactant: CDMT00098
fast: false
reversible: false
name: R31: Phosphorylation of Bfa1:Tem1GTP by cytosolic Kin4

CDMT00099
type: SpeciesReference
stoichiometry: 1.0
species: s53

CDMT00098
type: SpeciesReference
stoichiometry: 1.0
species: s50

active Bfa1 Cytosol
type: Parameter
constant: false
units: molecules
value: 0.0
name: Active Bfa1 in the cytosol

total Tem1 Cytosol
type: Parameter
constant: false
units: molecules
value: 0.0
name: Total Tem1 in the Cytosol

re30
type: Reaction
kinetic law: CDMT00154
product: CDMT00072
reactant: CDMT00071
reactant: CDMT00070
fast: false
reversible: true
name: R21: Rev. association of Tem1GDP with Cdc5-phosphorylated Bfa1

CDMT00072
type: SpeciesReference
stoichiometry: 1.0
species: s65

CDMT00071
type: SpeciesReference
stoichiometry: 1.0
species: s9

CDMT00070
type: SpeciesReference
stoichiometry: 1.0
species: s7

re44
type: Reaction
kinetic law: CDMT00161
product: CDMT00094
reactant: CDMT00093
reactant: CDMT00092
fast: false
reversible: true
name: R23: Rev. SPB-association of Tem1GDP

CDMT00094
type: SpeciesReference
stoichiometry: 1.0
species: s22

CDMT00093
type: SpeciesReference
stoichiometry: 1.0
species: s2

CDMT00092
type: SpeciesReference
stoichiometry: 1.0
species: s9

krKin4
type: Parameter
constant: true
units: ps
value: 0.0251

re49
type: Reaction
kinetic law: CDMT00163
product: CDMT00103
reactant: CDMT00102
fast: false
reversible: false
name: R30: Phosphorylation of Bfa1 by cytosolic Kin4

CDMT00103
type: SpeciesReference
stoichiometry: 1.0
species: s4

CDMT00102
type: SpeciesReference
stoichiometry: 1.0
species: s3

re5
type: Reaction
kinetic law: CDMT00133
product: CDMT00012
reactant: CDMT00011
reactant: CDMT00010
fast: false
reversible: true
name: R1: Rev. SPB-association of Bfa1

CDMT00012
type: SpeciesReference
stoichiometry: 1.0
species: s10

CDMT00011
type: SpeciesReference
stoichiometry: 1.0
species: s3

CDMT00010
type: SpeciesReference
stoichiometry: 1.0
species: s1

molar
type: SBMLUnitsDef
unit: CDMT00121
unit: CDMT00120
name: molar

CDMT00121
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: LITRE

CDMT00120
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: MOLE

re50
type: Reaction
kinetic law: CDMT00164
product: CDMT00105
reactant: CDMT00104
fast: false
reversible: false
name: R33: Dephosphorylation of Bfa1 by a yet unidentified phosphatase counteracting Kin4

CDMT00105
type: SpeciesReference
stoichiometry: 1.0
species: s3

CDMT00104
type: SpeciesReference
stoichiometry: 1.0
species: s4

koffB4
type: Parameter
constant: true
units: ps
value: 0.0365

re19
type: Reaction
kinetic law: CDMT00145
product: CDMT00047
reactant: CDMT00046
reactant: CDMT00045
fast: false
reversible: true
name: R10: Rev. association of Tem1GTP with SPB-bound Bfa1

CDMT00047
type: SpeciesReference
stoichiometry: 1.0
species: s25

CDMT00046
type: SpeciesReference
stoichiometry: 1.0
species: s10

CDMT00045
type: SpeciesReference
stoichiometry: 1.0
species: s8

active Tem1 Cytosol
type: Parameter
constant: false
units: molecules
value: 0.0
name: Active Tem1 in the cytosol

kfKin4Cyto
type: Parameter
constant: true
units: ps
value: 0.09

re25
type: Reaction
kinetic law: CDMT00150
product: CDMT00060
reactant: CDMT00059
reactant: CDMT00058
fast: false
reversible: true
name: R7: Rev. SPB-association of Bfa1-Tem1GDP complex

CDMT00060
type: SpeciesReference
stoichiometry: 1.0
species: s37

CDMT00059
type: SpeciesReference
stoichiometry: 1.0
species: s1

CDMT00058
type: SpeciesReference
stoichiometry: 1.0
species: s59

re59
type: Reaction
kinetic law: CDMT00173
product: CDMT00004
reactant: CDMT00003
reactant: CDMT00002
fast: false
reversible: true
name: R3: Rev. SPB-association of Cdc5-phosphorylated Bfa1

CDMT00004
type: SpeciesReference
stoichiometry: 1.0
species: s16

CDMT00003
type: SpeciesReference
stoichiometry: 1.0
species: s1

CDMT00002
type: SpeciesReference
stoichiometry: 1.0
species: s7

re29
type: Reaction
kinetic law: CDMT00153
product: CDMT00069
reactant: CDMT00068
reactant: CDMT00067
fast: false
reversible: true
name: R19: Rev. association of Tem1GDP with Bfa1

CDMT00069
type: SpeciesReference
stoichiometry: 1.0
species: s59

CDMT00068
type: SpeciesReference
stoichiometry: 1.0
species: s9

CDMT00067
type: SpeciesReference
stoichiometry: 1.0
species: s3

konB
type: Parameter
constant: true
units: pmps
value: 1250000.0

re38
type: Reaction
kinetic law: CDMT00158
product: CDMT00087
reactant: CDMT00086
fast: false
reversible: false
name: R47: GAP-accelerated GTP-hydrolysis

CDMT00087
type: SpeciesReference
stoichiometry: 1.0
species: s41

CDMT00086
type: SpeciesReference
stoichiometry: 1.0
species: s29

re40
type: Reaction
kinetic law: CDMT00160
product: CDMT00091
reactant: CDMT00090
fast: false
reversible: false
name: R48: GTP-hydrolysis with intrinisc GTPase activity

CDMT00091
type: SpeciesReference
stoichiometry: 1.0
species: s45

CDMT00090
type: SpeciesReference
stoichiometry: 1.0
species: s33

active Bfa1 SPB
type: Parameter
constant: false
units: molecules
value: 0.0
name: Active Bfa1 at the SPB

khyd
type: Parameter
constant: true
units: ps
value: 0.00224

total Bfa1 SPB
type: Parameter
constant: false
units: molecules
value: 0.0
name: Total Bfa1 at the SPB

konB4T
type: Parameter
constant: true
units: pmps
value: 3.65E7

W3 CDTF: Thu Feb 09 13:48:54 GMT 2012

ORG:
  orgname: Deutsches Krebsforschungszentrum Heidelberg
n:
  given: Maiko
  family: Lohel

W3 CDTF: Thu May 10 14:50:01 BST 2012

ORG:
  orgname: Deutsches Krebsforschungszentrum Heidelberg
n:
  given: Maiko
  family: Lohel

W3 CDTF: Tue Mar 06 17:02:12 GMT 2012

ORG:
  orgname: Deutsches Krebsforschungszentrum Heidelberg
n:
  given: Maiko
  family: Lohel