MODEL1007200000
type: SBMLModel
parameter: 761227
parameter: 761176
reaction: 761041
species: 760929
parameter: 761219
reaction: 760997
parameter: 761117
species: 760963
parameter: 761212
units def: 826144
parameter: 761109
parameter: 761091
parameter: 761188
name: Nijhout2006_Hepatic_Folate_Metab
parameter: 761283
species: 760943
units def: 825927
notes: This is the model described in the article: In silico experimentation with a model of hepatic mitochondrial folate metabolism. H. Frederik Nijhout, Michael C Reed, Shi-Ling Lam, Barry Shane, Jesse F Gregory and Cornelia M Ulrich, Theor Biol Med Model 2006,3:40; PubmedID: 17150100 ; DOI: 10.1186/1742-4682-3-40 ; Abstract: BACKGROUND: In eukaryotes, folate metabolism is compartmentalized and occurs in both the cytosol and the mitochondria. The function of this compartmentalization and the great changes that occur in the mitochondrial compartment during embryonic development and in rapidly growing cancer cells are gradually becoming understood, though many aspects remain puzzling and controversial. APPROACH: We explore the properties of cytosolic and mitochondrial folate metabolism by experimenting with a mathematical model of hepatic one-carbon metabolism. The model is based on known biochemical properties of mitochondrial and cytosolic enzymes. We use the model to study questions about the relative roles of the cytosolic and mitochondrial folate cycles posed in the experimental literature. We investigate: the control of the direction of the mitochondrial and cytosolic serine hydroxymethyltransferase (SHMT) reactions, the role of the mitochondrial bifunctional enzyme, the role of the glycine cleavage system, the effects of variations in serine and glycine inputs, and the effects of methionine and protein loading. CONCLUSION: The model reproduces many experimental findings and gives new insights into the underlying properties of mitochondrial folate metabolism. Particularly interesting is the remarkable stability of formate production in the mitochondria in the face of large changes in serine and glycine input. The model shows that in the presence of the bifunctional enzyme (as in embryonic tissues and cancer cells), the mitochondria primarily support cytosolic purine and pyrimidine synthesis via the export of formate, while in adult tissues the mitochondria produce se…
compartment: 760920
species: 760951
parameter: 761125
parameter: 761160
parameter: 761216
reaction: 761029
parameter: 761152
species: 760939
parameter: 761287
reaction: 761014
species: 760983
species: 760978
parameter: 761137
parameter: 761129
reaction: 760990
units def: 825951
parameter: 761156
species: 760927
species: 760958
species: 760966
parameter: 761231
parameter: 761092
species: 760935
function def: 761081
reaction: 761015
parameter: 761121
reaction: 760994
species: 760946
parameter: 761263
reaction: 761001
parameter: 761153
species: 760923
species: 760970
reaction: 761019
parameter: 761241
parameter: 761267
species: 760950
parameter: 761148
parameter: 761157
units def: 825987
reaction: 761016
reaction: 760988
reaction: 761045
parameter: 761166
parameter: 761244
parameter: 761261
parameter: 761124
reaction: 761049
reaction: 760985
reaction: 761020
parameter: 761248
parameter: 761128
length unit: 821056
parameter: 761203
reaction: 761032
volume units: 290956
reaction: 760989
reaction: 761024
parameter: 761291
parameter: 761120
parameter: 761207

761227
type: Parameter
constant: true
value: 22.0

761176
type: Parameter
constant: true
value: 10000.0

761041
type: Reaction
fast: false
product: 827659
reactant: 827647
product: 827671
reactant: 827635
modifier: 827683
kinetic law: 827696
reversible: true

827659
type: SpeciesReference
stoichiometry: 1.0
species: 760967

760967
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760917
name: S-adenosylhomocysteine

760917
type: Compartment
constant: true
size: 1.0
spatial dimensions: 3.0
name: cytosol

827647
type: SpeciesReference
stoichiometry: 1.0
species: 760962

760962
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760917
name: c_Glycine

827671
type: SpeciesReference
stoichiometry: 1.0
species: 760982

760982
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760921
name: Sarcosine

760921
type: Compartment
constant: true
size: 1.0
spatial dimensions: 3.0
name: whole cell

827635
type: SpeciesReference
stoichiometry: 1.0
species: 760970

760970
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760917
name: S-adenosylmethionine

760943
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760917
name: c_5-methyl-THF

760929
type: Species
constant: false
boundary condition: true
has only substance units: false
initial concentration: 50.0
initial amount: NaN
in compartment: 760917

761219
type: Parameter
constant: true
value: 43.0

760997
type: Reaction
kinetic law: 826486
product: 826474
reactant: 826462
reactant: 826450
fast: false
reversible: true

826474
type: SpeciesReference
stoichiometry: 1.0
species: 760954

760954
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760920
name: m_5-10-methylene-THF

760920
type: Compartment
constant: true
size: 1.0
spatial dimensions: 3.0
name: mitochondrion

826462
type: SpeciesReference
stoichiometry: 1.0
species: 760938

760938
type: Species
constant: false
boundary condition: true
has only substance units: false
initial concentration: 500.0
initial amount: NaN
in compartment: 760921
name: Formaldehyde

826450
type: SpeciesReference
stoichiometry: 1.0
species: 760942

760942
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: NaN
initial amount: NaN
in compartment: 760920

761117
type: Parameter
constant: true
value: 600.0

760963
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760917
name: Homocysteine

761212
type: Parameter
constant: true
value: 520.0

826144
type: SBMLUnitsDef
unit: 826157
name: hours

826157
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 3600.0
kind: SECOND

761109
type: Parameter
constant: true
value: 1050.0

761091
type: Parameter
constant: true
value: 2000.0

761188
type: Parameter
constant: true
value: 10000.0

761283
type: Parameter
constant: true
value: 10.0

825927
type: SBMLUnitsDef
unit: 825939
name: micromole

825939
type: SBMLUnit
exponent: 1.0
scale: -6
multipler: 1.0
kind: MOLE

760951
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760917

761125
type: Parameter
constant: true
value: 2000.0

761160
type: Parameter
constant: true
value: 2.0

761216
type: Parameter
constant: true
value: 3.0

761029
type: Reaction
kinetic law: 827380
product: 827368
product: 827356
reactant: 827344
fast: false
reversible: true

827368
type: SpeciesReference
stoichiometry: 1.0
species: 760929

827356
type: SpeciesReference
stoichiometry: 1.0
species: 760947

760947
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760917
name: c_5-10-methenyl-THF

827344
type: SpeciesReference
stoichiometry: 1.0
species: 760946

760946
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760917
name: c_5-10-methylene-THF

761152
type: Parameter
constant: true
value: 15000.0

760939
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: NaN
initial amount: NaN
in compartment: 760917

761287
type: Parameter
constant: true
value: 1.0

761014
type: Reaction
kinetic law: 826910
product: 826897
product: 826885
reactant: 826873
reactant: 826861
fast: false
reversible: true

826897
type: SpeciesReference
stoichiometry: 1.0
species: 760971

760971
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760917
name: methionine

826885
type: SpeciesReference
stoichiometry: 1.0
species: 760939

826873
type: SpeciesReference
stoichiometry: 1.0
species: 760963

826861
type: SpeciesReference
stoichiometry: 1.0
species: 760943

760983
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760921
name: Dimethylglycine

760978
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760920
name: mit_Glycine

761137
type: Parameter
constant: true
value: 20.0

761129
type: Parameter
constant: true
value: 43.0

760990
type: Reaction
kinetic law: 826328
product: 826316
reactant: 826304
fast: false
reversible: true

826316
type: SpeciesReference
stoichiometry: 1.0
species: 760942

826304
type: SpeciesReference
stoichiometry: 1.0
species: 760958

760958
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760920
name: m_10-formyl-THF

825951
type: SBMLUnitsDef
unit: 825975
unit: 825963
name: uM

825975
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: LITRE

825963
type: SBMLUnit
exponent: 1.0
scale: -6
multipler: 1.0
kind: MOLE

761156
type: Parameter
constant: true
value: 50.0

760927
type: Species
constant: false
boundary condition: true
has only substance units: false
initial concentration: 150.0
initial amount: NaN
in compartment: 760916

760916
type: Compartment
constant: true
size: 1.0
spatial dimensions: 3.0
name: blood

760966
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760917

761231
type: Parameter
constant: true
value: 6.3

761092
type: Parameter
constant: true
value: 150.0

760935
type: Species
constant: false
boundary condition: true
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760920
name: CO2

761015
type: Reaction
kinetic law: 826959
product: 826947
reactant: 826934
reactant: 826922
fast: false
reversible: true

826947
type: SpeciesReference
stoichiometry: 1.0
species: 760939

826934
type: SpeciesReference
stoichiometry: 1.0
species: 760929

826922
type: SpeciesReference
stoichiometry: 1.0
species: 760951

761121
type: Parameter
constant: true
value: 10000.0

760994
type: Reaction
kinetic law: 826437
product: 826425
reactant: 826413
reactant: 826401
fast: false
reversible: true

826425
type: SpeciesReference
stoichiometry: 1.0
species: 760958

826413
type: SpeciesReference
stoichiometry: 1.0
species: 760979

760979
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760920
name: m_formate

826401
type: SpeciesReference
stoichiometry: 1.0
species: 760942

761263
type: Parameter
constant: true
value: 360.0

761001
type: Reaction
kinetic law: 826608
product: 826595
product: 826583
reactant: 826571
reactant: 826559
fast: false
reversible: true

826595
type: SpeciesReference
stoichiometry: 1.0
species: 760978

826583
type: SpeciesReference
stoichiometry: 1.0
species: 760954

826571
type: SpeciesReference
stoichiometry: 1.0
species: 760982

826559
type: SpeciesReference
stoichiometry: 1.0
species: 760942

761153
type: Parameter
constant: true
value: 50.0

760923
type: Species
constant: false
boundary condition: true
has only substance units: false
initial concentration: 300.0
initial amount: NaN
in compartment: 760916

761019
type: Reaction
kinetic law: 827055
product: 827043
product: 827031
reactant: 827019
reactant: 827007
fast: false
reversible: true

827043
type: SpeciesReference
stoichiometry: 1.0
species: 760939

827031
type: SpeciesReference
stoichiometry: 1.0
species: 760959

760959
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760917
name: AICAR

827019
type: SpeciesReference
stoichiometry: 1.0
species: 760928

760928
type: Species
constant: false
boundary condition: true
has only substance units: false
initial concentration: 10.0
initial amount: NaN
in compartment: 760917

827007
type: SpeciesReference
stoichiometry: 1.0
species: 760950

760950
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760917
name: c_10-formyl-THF

761241
type: Parameter
constant: true
value: 80000.0

761267
type: Parameter
constant: true
value: 32.0

761148
type: Parameter
constant: true
value: 50.0

761157
type: Parameter
constant: true
value: 180000.0

825987
type: SBMLUnitsDef
unit: 826023
unit: 826011
unit: 825999
name: uM/h

826023
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 3600.0
kind: SECOND

826011
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: LITRE

825999
type: SBMLUnit
exponent: 1.0
scale: -6
multipler: 1.0
kind: MOLE

761016
type: Reaction
kinetic law: 826995
product: 826983
reactant: 826971
fast: false
reversible: true

826983
type: SpeciesReference
stoichiometry: 1.0
species: 760939

826971
type: SpeciesReference
stoichiometry: 1.0
species: 760950

760988
type: Reaction
kinetic law: 826255
product: 826243
reactant: 826230
fast: false
reversible: true

826243
type: SpeciesReference
stoichiometry: 1.0
species: 760966

826230
type: SpeciesReference
stoichiometry: 1.0
species: 760927

761045
type: Reaction
kinetic law: 827769
product: 827757
reactant: 827745
fast: false
reversible: true

827757
type: SpeciesReference
stoichiometry: 1.0
species: 760963

827745
type: SpeciesReference
stoichiometry: 1.0
species: 760967

761166
type: Parameter
constant: true
value: 790000.0

761244
type: Parameter
constant: true
value: 500000.0

761261
type: Parameter
constant: true
value: 220.0

761124
type: Parameter
constant: true
value: 3200.0

761049
type: Reaction
modifier: 827829
modifier: 827853
modifier: 827865
kinetic law: 827878
reactant: 827817
reactant: 827805
modifier: 827841
fast: false
reversible: true

827817
type: SpeciesReference
stoichiometry: 1.0
species: 760966

827805
type: SpeciesReference
stoichiometry: 1.0
species: 760963

760985
type: Reaction
kinetic law: 826218
product: 826206
reactant: 826194
fast: false
reversible: true

826206
type: SpeciesReference
stoichiometry: 1.0
species: 760962

826194
type: SpeciesReference
stoichiometry: 1.0
species: 760923

761020
type: Reaction
kinetic law: 827104
product: 827092
reactant: 827080
reactant: 827067
fast: false
reversible: true

827092
type: SpeciesReference
stoichiometry: 1.0
species: 760950

827080
type: SpeciesReference
stoichiometry: 1.0
species: 760974

760974
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760917
name: c_formate

827067
type: SpeciesReference
stoichiometry: 1.0
species: 760939

761248
type: Parameter
constant: true
value: 2160.0

761128
type: Parameter
constant: true
value: 3.0

761203
type: Parameter
constant: true
value: 0.5

761032
type: Reaction
kinetic law: 827416
product: 827404
reactant: 827392
fast: false
reversible: true

827404
type: SpeciesReference
stoichiometry: 1.0
species: 760950

827392
type: SpeciesReference
stoichiometry: 1.0
species: 760947

760989
type: Reaction
kinetic law: 826291
product: 826280
reactant: 826267
fast: false
reversible: true

826280
type: SpeciesReference
stoichiometry: 1.0
species: 760971

826267
type: SpeciesReference
stoichiometry: 1.0
species: 760922

760922
type: Species
constant: false
boundary condition: true
has only substance units: false
initial concentration: 30.0
initial amount: NaN
in compartment: 760916

761024
type: Reaction
kinetic law: 827212
product: 827200
reactant: 827188
reactant: 827176
fast: false
reversible: true

827200
type: SpeciesReference
stoichiometry: 1.0
species: 760946

827188
type: SpeciesReference
stoichiometry: 1.0
species: 760938

827176
type: SpeciesReference
stoichiometry: 1.0
species: 760939

761291
type: Parameter
constant: true
value: 1000.0

761120
type: Parameter
constant: true
value: 3.0E7

761207
type: Parameter
constant: true
value: 500.0

761036
type: Reaction
reversible: true
product: 827501
modifier: 827538
kinetic law: 827551
modifier: 827525
reactant: 827489
fast: false
reactant: 827477
product: 827513

827501
type: SpeciesReference
stoichiometry: 1.0
species: 760971

827489
type: SpeciesReference
stoichiometry: 1.0
species: 760930

760930
type: Species
constant: false
boundary condition: true
has only substance units: false
initial concentration: 50.0
initial amount: NaN
in compartment: 760917
name: Betaine

827477
type: SpeciesReference
stoichiometry: 1.0
species: 760963

827513
type: SpeciesReference
stoichiometry: 1.0
species: 760983

760998
type: Reaction
kinetic law: 826547
product: 826535
product: 826523
reactant: 826510
reactant: 826498
fast: false
reversible: true

826535
type: SpeciesReference
stoichiometry: 1.0
species: 760935

826523
type: SpeciesReference
stoichiometry: 1.0
species: 760954

826510
type: SpeciesReference
stoichiometry: 1.0
species: 760978

826498
type: SpeciesReference
stoichiometry: 1.0
species: 760942

761082
type: Parameter
constant: false
value: NaN

761033
type: Reaction
kinetic law: 827465
product: 827452
reactant: 827440
reactant: 827428
fast: false
reversible: true

827452
type: SpeciesReference
stoichiometry: 1.0
species: 760939

827440
type: SpeciesReference
stoichiometry: 1.0
species: 760959

827428
type: SpeciesReference
stoichiometry: 1.0
species: 760950

761005
type: Reaction
kinetic law: 826704
product: 826692
reactant: 826680
fast: false
reversible: true

826692
type: SpeciesReference
stoichiometry: 1.0
species: 760955

760955
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760920
name: m_5-10-methenyl-THF

826680
type: SpeciesReference
stoichiometry: 1.0
species: 760954

761246
type: Parameter
constant: true
value: 5.9

761100
type: Parameter
constant: true
value: 1.0

761037
type: Reaction
kinetic law: 827587
product: 827575
reactant: 827563
fast: false
reversible: true

827575
type: SpeciesReference
stoichiometry: 1.0
species: 760970

827563
type: SpeciesReference
stoichiometry: 1.0
species: 760971

761140
type: Parameter
constant: true
value: 15000.0

760931
type: Species
constant: false
boundary condition: true
has only substance units: false
initial concentration: 20.0
initial amount: NaN
in compartment: 760917
name: dUMP

761009
type: Reaction
kinetic law: 826776
product: 826764
reactant: 826752
fast: false
reversible: true

826764
type: SpeciesReference
stoichiometry: 1.0
species: 760966

826752
type: SpeciesReference
stoichiometry: 3.0
species: 760975

760975
type: Species
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 760920
name: m_Serine

761132
type: Parameter
constant: true
value: 6300.0

761292
type: Parameter
constant: true
value: 2000.0

761284
type: Parameter
constant: true
value: 5000.0

761104
type: Parameter
constant: true
value: 910.0

761192
type: Parameter
constant: true
value: 500.0

761144
type: Parameter
constant: true
value: 3400.0

761095
type: Parameter
constant: true
value: 1.0

761288
type: Parameter
constant: true
value: 402000.0

761108
type: Parameter
constant: true
value: 1.0

761183
type: Parameter
constant: true
value: 100.0

761099
type: Parameter
constant: true
value: 150.0

761196
type: Parameter
constant: true
value: 1.0

761040
type: Reaction
kinetic law: 827623
product: 827611
reactant: 827599
fast: false
reversible: true

827611
type: SpeciesReference
stoichiometry: 1.0
species: 760970

827599
type: SpeciesReference
stoichiometry: 1.0
species: 760971

826035
type: SBMLUnitsDef
unit: 826047
name: 1/h

826047
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 3600.0
kind: SECOND

761084
type: Parameter
constant: true
value: 0.0

760934
type: Species
constant: false
boundary condition: true
has only substance units: false
initial concentration: 20.0
initial amount: NaN
in compartment: 760921
name: Folate

761133
type: Parameter
constant: true
value: 22.0

761010
type: Reaction
kinetic law: 826812
product: 826800
reactant: 826788
fast: false
reversible: true

826800
type: SpeciesReference
stoichiometry: 1.0
species: 760974

826788
type: SpeciesReference
stoichiometry: 3.0
species: 760979

761187
type: Parameter
constant: true
value: 5700.0

761282
type: Parameter
constant: true
value: 5000.0

761096
type: Parameter
constant: true
value: 2700.0

761088
type: Parameter
constant: true
value: 2.71828

761028
type: Reaction
modifier: 827320
modifier: 827308
reactant: 827284
fast: false
product: 827296
kinetic law: 827332
reactant: 827272
reversible: true

827284
type: SpeciesReference
stoichiometry: 1.0
species: 760929

827296
type: SpeciesReference
stoichiometry: 1.0
species: 760943

827272
type: SpeciesReference
stoichiometry: 1.0
species: 760946

761220
type: Parameter
constant: true
value: 5200.0

761011
type: Reaction
kinetic law: 826849
product: 826837
reactant: 826824
fast: false
reversible: true

826837
type: SpeciesReference
stoichiometry: 1.0
species: 760962

826824
type: SpeciesReference
stoichiometry: 3.0
species: 760978

761149
type: Parameter
constant: true
value: 320.0

761245
type: Parameter
constant: true
value: 55000.0

761224
type: Parameter
constant: true
value: 0.03

761251
type: Parameter
constant: true
value: 12.0

761256
type: Parameter
constant: true
value: 0.01

760993
type: Reaction
kinetic law: 826389
product: 826377
product: 826365
reactant: 826353
reactant: 826340
fast: false
reversible: true

826377
type: SpeciesReference
stoichiometry: 1.0
species: 760954

826365
type: SpeciesReference
stoichiometry: 1.0
species: 760978

826353
type: SpeciesReference
stoichiometry: 1.0
species: 760975

826340
type: SpeciesReference
stoichiometry: 1.0
species: 760942

761270
type: Parameter
constant: true
value: 130.0

761167
type: Parameter
constant: true
value: 250.0

761021
type: Reaction
kinetic law: 827164
product: 827152
product: 827140
reactant: 827128
reactant: 827116
fast: false
reversible: true

827152
type: SpeciesReference
stoichiometry: 1.0
species: 760946

827140
type: SpeciesReference
stoichiometry: 1.0
species: 760962

827128
type: SpeciesReference
stoichiometry: 1.0
species: 760939

827116
type: SpeciesReference
stoichiometry: 1.0
species: 760966

761262
type: Parameter
constant: true
value: 300.0

761228
type: Parameter
constant: true
value: 5000.0

826059
type: SBMLUnitsDef
unit: 826095
unit: 826083
unit: 826071
name: 1/(uM*h)

826095
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 3600.0
kind: SECOND

826083
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: LITRE

826071
type: SBMLUnit
exponent: -1.0
scale: -6
multipler: 1.0
kind: MOLE

761191
type: Parameter
constant: true
value: 5700.0

761170
type: Parameter
constant: true
value: 20000.0

761200
type: Parameter
constant: true
value: 2000.0

761240
type: Parameter
constant: true
value: 16.0

761257
type: Parameter
constant: true
value: 260.0

761025
type: Reaction
kinetic law: 827260
product: 827248
reactant: 827236
reactant: 827224
fast: false
reversible: true

827248
type: SpeciesReference
stoichiometry: 1.0
species: 760951

827236
type: SpeciesReference
stoichiometry: 1.0
species: 760946

827224
type: SpeciesReference
stoichiometry: 1.0
species: 760931

761266
type: Parameter
constant: true
value: 245.0

761271
type: Parameter
constant: true
value: 18.0

826108
type: SBMLUnitsDef
unit: 826132
unit: 826120
name: 1/uM

826132
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: LITRE

826120
type: SBMLUnit
exponent: -1.0
scale: -6
multipler: 1.0
kind: MOLE

761195
type: Parameter
constant: true
value: 25.0

761161
type: Parameter
constant: true
value: 600000.0

761252
type: Parameter
constant: true
value: 100.0

761204
type: Parameter
constant: true
value: 4.0

761235
type: Parameter
constant: true
value: 5300.0

761083
type: Parameter
constant: false
value: NaN

761275
type: Parameter
constant: true
value: 180.0

761002
type: Reaction
kinetic law: 826668
product: 826656
product: 826644
reactant: 826632
reactant: 826620
fast: false
reversible: true

826656
type: SpeciesReference
stoichiometry: 1.0
species: 760982

826644
type: SpeciesReference
stoichiometry: 1.0
species: 760954

826632
type: SpeciesReference
stoichiometry: 1.0
species: 760983

826620
type: SpeciesReference
stoichiometry: 1.0
species: 760942

761199
type: Parameter
constant: true
value: 0.01

761112
type: Parameter
constant: true
value: 20.0

761044
type: Reaction
kinetic law: 827732
product: 827720
reactant: 827708
fast: false
reversible: true

827720
type: SpeciesReference
stoichiometry: 1.0
species: 760967

827708
type: SpeciesReference
stoichiometry: 1.0
species: 760970

761165
type: Parameter
constant: true
value: 10.0

761281
type: Parameter
constant: true
value: 1.4

761247
type: Parameter
constant: true
value: 100.0

761208
type: Parameter
constant: true
value: 24300.0

761171
type: Parameter
constant: true
value: 100.0

761087
type: Parameter
constant: true
value: 0.0

761232
type: Parameter
constant: true
value: 14.0

761006
type: Reaction
kinetic law: 826740
product: 826728
reactant: 826716
fast: false
reversible: true

826728
type: SpeciesReference
stoichiometry: 1.0
species: 760958

826716
type: SpeciesReference
stoichiometry: 1.0
species: 760955

761258
type: Parameter
constant: true
value: 41.0

761116
type: Parameter
constant: true
value: 50.0

761048
type: Reaction
kinetic law: 827793
reactant: 827781
fast: false
reversible: true

827781
type: SpeciesReference
stoichiometry: 1.0
species: 760966

761211
type: Parameter
constant: true
value: 4.9

761175
type: Parameter
constant: true
value: 5700.0

761236
type: Parameter
constant: true
value: 50.0

761180
type: Parameter
constant: true
value: 100.0

761141
type: Parameter
constant: true
value: 50.0

761223
type: Parameter
constant: true
value: 1.5E7

761172
type: Parameter
constant: true
value: 10000.0

761215
type: Parameter
constant: true
value: 3900.0

761276
type: Parameter
constant: true
value: 1.4

761113
type: Parameter
constant: true
value: 11440.0

761105
type: Parameter
constant: true
value: 150.0

761179
type: Parameter
constant: true
value: 5700.0

761184
type: Parameter
constant: true
value: 10000.0

761136
type: Parameter
constant: true
value: 0.03

761145
type: Parameter
constant: true
value: 15000.0