BIOMD0000000353
type: SBMLModel
reaction: G5 degradation
units def: 498119
units def: 498339
compartment: 032811
parameter: 033079
parameter: 033040
species: CHK
parameter: DPE2 KMA
parameter: DPE2 KMB
species: DPE1
parameter: 033059
parameter: 032940
parameter: DPE2 KMP
parameter: CGP KiA
parameter: 033072
species: 032822
is version of: GO:0005983
parameter: 033064
parameter: CGP Keq
compartment: 032810
parameter: CGP KMQ
length unit: length
time units: metaid_0000075
parameter: DPE2 KiQ
species: 032834
parameter: 033052
units def: 498375
parameter: DPE1 Keq
parameter: 032947
reaction: G3 formation
parameter: 033071
units def: 498071
species: 032846
parameter: 033008
compartment: 032805
parameter: CGP KMP
compartment: 032806
species: 032817
parameter: 033047
units def: 498205
rule: 032926
parameter: 033051
species: 032850
name: Nag2011_ChloroplasticStarchDegradation
parameter: 033034
occurs in: http://identifiers.org/taxonomy/3701
notes: This model is from the article: Kinetic modeling and exploratory numerical simulation of chloroplastic starch degradation. Nag A, Lunacek M, Graf PA, Chang CH. BMC Syst Biol. 2011 Jun 18;5:94. 21682905 , Abstract: BACKGROUND: Higher plants and algae are able to fix atmospheric carbon dioxide through photosynthesis and store this fixed carbon in large quantities as starch, which can be hydrolyzed into sugars serving as feedstock for fermentation to biofuels and precursors. Rational engineering of carbon flow in plant cells requires a greater understanding of how starch breakdown fluxes respond to variations in enzyme concentrations, kinetic parameters, and metabolite concentrations. We have therefore developed and simulated a detailed kinetic ordinary differential equation model of the degradation pathways for starch synthesized in plants and green algae, which to our knowledge is the most complete such model reported to date. RESULTS: Simulation with 9 internal metabolites and 8 external metabolites, the concentrations of the latter fixed at reasonable biochemical values, leads to a single reference solution showing β-amylase activity to be the rate-limiting step in carbon flow from starch degradation. Additionally, the response coefficients for stromal glucose to the glucose transporter kcat and KM are substantial, whereas those for cytosolic glucose are not, consistent with a kinetic bottleneck due to transport. Response coefficient norms show stromal maltopentaose and cytosolic glucosylated arabinogalactan to be the most and least globally sensitive metabolites, respectively, and β-amylase kcat and KM for starch to be the kinetic parameters with the largest aggregate effect on metabolite concentrations as a whole. The latter kinetic parameters, together with those for glucose transport, have the greatest effect on stromal glucose, which is a precursor for biofuel synthetic pathways. Exploration of the steady-state solution space with respect to concentrations of …
parameter: CGP KMB
parameter: DPE1 KMP
reaction: G2 transport
parameter: DPE1 KMQ
species: 032826
parameter: 032939
species: 032838
is: MODEL1106030000
reaction: DPE1 RXN
species: pGlcT
parameter: 033039
units def: 498302
species: 032830
compartment: 032814
units def: 498143
species: MEX
species: DPE2
reaction: CGP RXN
parameter: 033044
species: 032842
parameter: 033082
parameter: 033083
species: 032823
area units: 498119
parameter: 032944
reaction: G2 formation
species: 032818
species: 032835
compartment: 032804
parameter: CGP KMA
parameter: 033076
parameter: 033068

G5 degradation
type: Reaction
modifier: 498644
product: 498632
is: SBO:0000179
reversible: false
product: 498620
reactant: 498608
fast: false
name: Maltopentaose degradation
kinetic law: 498656

498644
type: ModifierSpeciesReference
species: beta amylase
name: beta amylase

beta amylase
type: Species
in compartment: 032814
boundary condition: false
name: Beta amylase
is: http://identifiers.org/ec-code/3.2.1.2
initial amount: NaN
substance units: gram
constant: false
is: SBO:0000014
has only substance units: false
initial concentration: 0.00783

032814
type: Compartment
is version of: GO:0009570
is: SBO:0000290
constant: true
units: 498095
size: 1.0
spatial dimensions: 3.0
name: Chloroplast Stroma

498107
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: LITRE

498632
type: SpeciesReference
stoichiometry: 1984.13
species: 032823
name: Maltotriose

032823
type: Species
constant: false
has only substance units: false
is: SBO:0000247
is part of: C01835
boundary condition: false
initial concentration: 100.0
is part of: CHEBI:61993
name: Maltotriose
in compartment: 032814
substance units: 498143
initial amount: NaN

498155
type: SBMLUnit
exponent: 1.0
scale: -6
multipler: 1.0
kind: MOLE

498620
type: SpeciesReference
stoichiometry: 2923.98
species: 032822
name: Maltose

032822
type: Species
in compartment: 032814
initial concentration: 10.0
initial amount: NaN
boundary condition: false
constant: false
has only substance units: false
is version of: C00208
substance units: 498143
is: SBO:0000247
name: Maltose
is version of: CHEBI:17306

498608
type: SpeciesReference
stoichiometry: 1207.73
species: 032826
name: Maltopentaose

032826
type: Species
boundary condition: false
is version of: CHEBI:62006
in compartment: 032814
has only substance units: false
constant: false
initial amount: NaN
substance units: 498143
name: Maltopentaose
is: SBO:0000247
initial concentration: 1000.0

498131
type: SBMLUnit
exponent: 2.0
scale: -6
multipler: 1.0
kind: METRE

498363
type: SBMLUnit
exponent: -2.0
scale: 0
multipler: 1.0
kind: LITRE

498351
type: SBMLUnit
exponent: 2.0
scale: 0
multipler: 1.0
kind: GRAM

032811
type: Compartment
is version of: GO:0009706
is: SBO:0000290
constant: true
units: 498119
size: 1000.0
spatial dimensions: 2.0
name: Chloroplast Inner Membrane

033079
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 10.0
name: Hexokinase Ki(G6P),2

498192
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: LITRE

498180
type: SBMLUnit
exponent: 1.0
scale: -6
multipler: 1.0
kind: MOLE

033040
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 19300.0
name: Glucose transporter (pGlcT) KM

CHK
type: Species
boundary condition: false
initial concentration: 10.0
has only substance units: false
constant: false
is: http://identifiers.org/ec-code/2.7.1.1
initial amount: NaN
in compartment: 032805
substance units: 498143
is: SBO:0000014
name: Hexokinase

032805
type: Compartment
is version of: GO:0005829
is: SBO:0000290
constant: false
units: 498095
size: 1.0
spatial dimensions: 3.0
name: Cytosol

DPE2 KMA
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 4600.0
name: DPE2 Maltose KM

DPE2 KMB
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 1100.0
name: DPE2 AG KM

DPE1
type: Species
is: http://identifiers.org/ec-code/2.4.1.25
initial concentration: 2.0
boundary condition: false
constant: false
has only substance units: false
initial amount: NaN
in compartment: 032814
name: Disproportionating enzyme 1(DPE1)
substance units: 498143
is: SBO:0000014

033059
type: Parameter
is: SBO:0000321
constant: false
units: 498071
value: NaN
name: Hexokinase kcat,reverse

498083
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND

032940
type: Parameter
is: SBO:0000504
constant: true
units: 498375
value: 8.28E-4
name: Maltopentaose Mol. Wt.

498399
type: SBMLUnit
exponent: -1.0
scale: -6
multipler: 1.0
kind: MOLE

498387
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: GRAM

DPE2 KMP
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 11700.0
name: DPE2 Glc KM

CGP KiA
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 3800.0
name: Cytosolic glucan phosphorylase Glucosyl Arabinogalactan Ki

033072
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 47.0
name: Hexokinase Ki(G6P)

033064
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 1000.0
name: Hexokinase Ki(MgATP)

CGP Keq
type: Parameter
is: SBO:0000281
constant: true
units: dimensionless
value: 6.15E-4
name: Cytosolic glucan phosphorylase Keq

032810
type: Compartment
is version of: GO:0031972
is: SBO:0000290
constant: true
units: 498095
size: 1.0
spatial dimensions: 3.0
name: Chloroplast Intermembrane Space

CGP KMQ
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 3800.0
name: Cytosolic glucan phosphorylase Arabinogalactan KM

DPE2 KiQ
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 1000.0
name: DPE2 GlcAG Ki

032834
type: Species
is version of: MOD:00726
is version of: C00569
initial amount: NaN
boundary condition: false
in compartment: 032805
constant: false
name: Glucosyl Arabinogalactan (GlcAG)
substance units: 498143
is: SBO:0000247
has only substance units: false
is version of: CHEBI:27569
initial concentration: 10000.0

033052
type: Parameter
is: SBO:0000025
constant: true
units: 498071
value: 0.0913
name: kcat_betaamylase_Maltopentaose

DPE1 Keq
type: Parameter
is: SBO:0000281
constant: true
units: dimensionless
value: 1.0
name: DPE1 Keq

032947
type: Parameter
is: SBO:0000025
constant: true
units: 498071
value: 50.0
name: DPE1 kcat

G3 formation
type: Reaction
fast: false
modifier: 498583
product: 498546
is: SBO:0000393
reactant: 498534
reversible: false
modifier: 498571
kinetic law: 498595
name: Maltotriose formation from Starch
modifier: 498559

498583
type: ModifierSpeciesReference
species: 032818
name: Starch DB

032818
type: Species
has only substance units: false
is version of: CHEBI:28017
initial concentration: 0.0
is version of: C00369
constant: false
is version of: MOD:00726
in compartment: 032814
initial amount: NaN
name: Starch exposed to Beta Amylase due to action of Isoamylase (Starch DB)
boundary condition: false
is: SBO:0000247
substance units: gram

498546
type: SpeciesReference
stoichiometry: 1984.13
species: 032823
name: Maltotriose

498534
type: SpeciesReference
stoichiometry: 18518.5
species: 032817
name: Starch Glucosyl unit

032817
type: Species
substance units: 498143
in compartment: 032814
has only substance units: false
is: SBO:0000247
constant: false
initial amount: NaN
is version of: C00369
is version of: CHEBI:28017
is version of: MOD:00726
boundary condition: true
name: Starch Glucosyl unit
initial concentration: 1000.0

498571
type: ModifierSpeciesReference
species: 032816
name: Starch

032816
type: Species
is: SBO:0000247
is version of: CHEBI:28017
constant: false
substance units: gram
initial amount: NaN
name: Starch
is version of: C00369
initial concentration: NaN
in compartment: 032814
boundary condition: false
has only substance units: false

498559
type: ModifierSpeciesReference
species: beta amylase
name: beta amylase

033071
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 47.0
name: Hexokinase KM(G6P)

032846
type: Species
initial concentration: 10000.0
is version of: CHEBI:19324
constant: false
substance units: 498143
initial amount: NaN
is: SBO:0000247
name: 2,3-Bisphosphoglycerate pool
has only substance units: false
boundary condition: false
is version of: C03339
in compartment: 032805

033008
type: Parameter
is: SBO:0000025
constant: true
units: 498071
value: 50.0
name: Cytosolic glucan phosphorylase kcat

CGP KMP
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 2000.0
name: Cytosolic glucan phosphorylase G1P KM

032806
type: Compartment
is version of: GO:0009507
is: SBO:0000290
constant: false
units: 498095
size: 1.0
spatial dimensions: 3.0
name: Chloroplast

033047
type: Parameter
is: SBO:0000027
constant: true
units: 498339
value: 4.19
name: Beta Amylase KM for Maltose condensation

498241
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND

498229
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: LITRE

498217
type: SBMLUnit
exponent: 1.0
scale: -6
multipler: 1.0
kind: MOLE

033051
type: Parameter
is: SBO:0000025
constant: true
units: 498071
value: 0.073
name: kcat_betaamylase_Starch

032850
type: Species
initial concentration: 10000.0
name: G6P pool
is: SBO:0000247
is version of: C00668
is version of: CHEBI:17665
substance units: 498143
initial amount: NaN
boundary condition: true
constant: false
in compartment: 032805
has only substance units: false

033034
type: Parameter
is: SBO:0000025
constant: true
units: 498071
value: 5.963
name: Maltose exporter (MEX) kcat

CGP KMB
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 5900.0
name: Cytosolic glucan phosphorylase Pi KM

DPE1 KMP
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 11700.0
name: DPE1 Glc KM

G2 transport
type: Reaction
kinetic law: 498705
modifier: 498692
product: 498680
reactant: 498668
is: SBO:0000185
fast: false
reversible: false
name: Maltose exporter

498692
type: ModifierSpeciesReference
species: MEX
name: Maltose exporter(MEX)

MEX
type: Species
is: 2.A.84.1.2
boundary condition: false
substance units: 498143
has only substance units: false
in compartment: 032810
name: Maltose exporter (MEX)
initial amount: NaN
is: SBO:0000284
initial concentration: 2.0
constant: false

498680
type: SpeciesReference
stoichiometry: 1.0
species: 032819
name: Maltose

032819
type: Species
is version of: C00208
boundary condition: false
constant: false
is: SBO:0000247
substance units: 498143
name: Maltose
is version of: CHEBI:17306
initial amount: NaN
in compartment: 032805
initial concentration: 10.0
has only substance units: false

498668
type: SpeciesReference
stoichiometry: 1.0
species: 032822
name: Maltose

DPE1 KMQ
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 210.0
name: DPE1 G5 KM

032939
type: Parameter
is: SBO:0000504
constant: true
units: 498375
value: 0.206
name: Beta amylase molecular weight

032838
type: Species
is version of: C00008
name: ADP pool
is version of: CHEBI:16761
in compartment: 032805
has only substance units: false
constant: false
boundary condition: true
substance units: 498143
is: SBO:0000247
initial concentration: 10000.0
initial amount: NaN

DPE1 RXN
type: Reaction
reactant: 499033
modifier: 499069
name: DPE1
is: SBO:0000559
reversible: true
product: 499045
product: 499057
kinetic law: 499081
fast: false

499033
type: SpeciesReference
stoichiometry: 2.0
species: 032823
name: Maltotriose

499069
type: ModifierSpeciesReference
species: DPE1
name: DPE1

499045
type: SpeciesReference
stoichiometry: 1.0
species: 032827
name: D-Glucose

032827
type: Species
in compartment: 032814
has only substance units: false
constant: false
substance units: 498143
is version of: CHEBI:17634
name: (D)-Glucose
boundary condition: false
initial amount: NaN
is: SBO:0000247
initial concentration: 10.0

499057
type: SpeciesReference
stoichiometry: 1.0
species: 032826
name: Maltopentaose

pGlcT
type: Species
initial amount: NaN
boundary condition: false
substance units: 498143
name: Glucose transporter (pGlcT)
is: 2.A.1.1.17
has only substance units: false
in compartment: 032810
is: SBO:0000284
initial concentration: 20.0
constant: false

033039
type: Parameter
is: SBO:0000025
constant: true
units: 498071
value: 240.278
name: Glucose transporter (pGlcT) kcat

498327
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: LITRE

498314
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: GRAM

032830
type: Species
boundary condition: false
initial concentration: 10.0
in compartment: 032805
is version of: CHEBI:17634
is version of: C00031
is: SBO:0000247
name: (D)-Glucose
has only substance units: false
substance units: 498143
constant: false
initial amount: NaN

DPE2
type: Species
constant: false
has only substance units: false
initial amount: NaN
initial concentration: 2.0
name: Disproportionating enzyme 2(DPE2)
boundary condition: false
is: SBO:0000014
in compartment: 032805
is: http://identifiers.org/ec-code/2.4.1.25
substance units: 498143

CGP RXN
type: Reaction
reactant: 498900
reactant: 498888
modifier: 498936
fast: false
product: 498912
kinetic law: 498948
reversible: true
is: SBO:0000559
product: 498924
name: Cytosolic glucan phosphorylase

498900
type: SpeciesReference
stoichiometry: 1.0
species: 032839
name: HPi

032839
type: Species
boundary condition: true
initial amount: NaN
is version of: CHEBI:18367
name: Orthophosphate(HPi) pool
constant: false
is version of: C00009
substance units: 498143
in compartment: 032805
has only substance units: false
initial concentration: 10000.0
is: SBO:0000247

498888
type: SpeciesReference
stoichiometry: 1.0
species: 032834
name: Glucosyl Arabinogalactan

498936
type: ModifierSpeciesReference
species: CGP
name: Cytosolic glucan phosphorylase

CGP
type: Species
has only substance units: false
constant: false
substance units: 498143
boundary condition: false
in compartment: 032805
is: http://identifiers.org/ec-code/2.4.1.1
name: Cytosolic Glucan phosphorylase
initial concentration: 2.0
initial amount: NaN
is: SBO:0000014

498912
type: SpeciesReference
stoichiometry: 1.0
species: 032847
name: G1P pool

032847
type: Species
is version of: C11450
initial amount: NaN
is: SBO:0000247
name: G1P pool
has only substance units: false
initial concentration: 10000.0
is version of: CHEBI:29042
in compartment: 032805
substance units: 498143
boundary condition: true
constant: false

498924
type: SpeciesReference
stoichiometry: 1.0
species: 032831
name: Arabinogalactan

032831
type: Species
constant: false
boundary condition: false
name: Arabinogalactan (AG)
initial amount: NaN
has only substance units: false
is version of: C00569
is version of: CHEBI:27569
in compartment: 032805
substance units: 498143
is: SBO:0000247
initial concentration: 10000.0

033044
type: Parameter
is: SBO:0000027
constant: true
units: 498302
value: 1.46
name: Beta Amylase KM for Maltopentaose

032842
type: Species
initial amount: NaN
is version of: CHEBI:16856
is version of: C00051
boundary condition: false
is: SBO:0000247
has only substance units: false
substance units: 498143
constant: false
initial concentration: 1000.0
name: Glutathione (reduced)
in compartment: 032805

033082
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 4000.0
name: Hexokinase Ki(2,3-bisphosphoglycerate)

033083
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 30.0
name: Hexokinase Ki(glucose 1,6-bisphosphate

032944
type: Parameter
is: SBO:0000504
constant: true
units: 498375
value: 3.42E-4
name: Maltose Mol. Wt.

G2 formation
type: Reaction
reactant: 498459
name: Maltose formation from Starch
modifier: 498496
product: 498472
is: SBO:0000393
modifier: 498509
kinetic law: 498521
modifier: 498484
fast: false
reversible: true

498459
type: SpeciesReference
stoichiometry: 12345.7
species: 032817
name: Starch Glucosyl unit

498496
type: ModifierSpeciesReference
species: 032816
name: Starch

498472
type: SpeciesReference
stoichiometry: 2923.98
species: 032822
name: Maltose

498509
type: ModifierSpeciesReference
species: 032818
name: Starch DB

498484
type: ModifierSpeciesReference
species: beta amylase
name: beta amylase

032835
type: Species
boundary condition: true
substance units: 498143
initial amount: NaN
name: ATP pool
is version of: C00002
initial concentration: 10000.0
constant: false
is: SBO:0000247
in compartment: 032805
is version of: CHEBI:15422
has only substance units: false

032804
type: Compartment
is version of: GO:0005623
is: SBO:0000290
constant: true
units: 498095
size: 3.534E-12
spatial dimensions: 3.0
name: Cell

CGP KMA
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 2100.0
name: Cytosolic glucan phosphorylase Glucosyl Arabinogalactan KM

033076
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 1000.0
name: Hexokinase Ki(MgADP)

033068
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 47.0
name: Hexokinase Ki(glucose)

032931
type: Parameter
is: SBO:0000540
constant: true
units: dimensionless
value: 0.582
name: Fraction of Starch amenable to degradation by Beta Amylase only

033063
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 1000.0
name: Hexokinase KM(MgATP)

032932
type: Parameter
is: SBO:0000540
constant: true
units: dimensionless
value: 0.87
name: Fraction of starch degraded to Maltose

DPE2 KiA
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 2190.476
name: DPE2 Maltose Ki

DPE1 KiP
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 5571.429
name: DPE1 Glc Ki

DPE1 KiQ
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 100.0
name: DPE1 G5 Ki

DPE1 KMA
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 3300.0
name: DPE1 G3 KM

498290
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND

498278
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: LITRE

498266
type: SBMLUnit
exponent: -1.0
scale: -6
multipler: 1.0
kind: MOLE

DPE2 KiP
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 5571.429
name: DPE2 Glc Ki

033056
type: Parameter
is: SBO:0000320
constant: true
units: 498071
value: 180.0
name: Hexokinase kcat,forward

033075
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 1000.0
name: Hexokinase KM(MgADP)

CGP KiP
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 3100.0
name: Cytosolic glucan phosphorylase G1P Ki

iso amylase
type: Species
in compartment: 032814
substance units: gram
constant: false
initial concentration: 0.0118
has only substance units: false
initial amount: NaN
boundary condition: false
is: http://identifiers.org/ec-code/3.2.1.68
name: Isoamylase
is: SBO:0000014

032815
type: Species
has only substance units: false
initial concentration: 0.1
is version of: CHEBI:24636
constant: false
initial amount: NaN
boundary condition: true
is version of: C00080
name: H+
is: SBO:0000327
substance units: 498143
in compartment: 032805

DPE2 KiB
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 1000.0
name: DPE2 AG Ki

W3 CDTF: Tue Jan 31 13:37:23 GMT 2012

HK RXN
type: Reaction
kinetic law: 498876
modifier: 498864
fast: false
modifier: 498852
product: 498815
name: Hexokinase
product: 498791
reversible: true
is: SBO:0000559
product: 498803
reactant: 498766
modifier: 498839
notes: Kinetics reversible random sequential bi-bi.
reactant: 498778
modifier: 498827

498864
type: ModifierSpeciesReference
species: 032846
name: 2,3-Bisphosphoglycerate pool

498852
type: ModifierSpeciesReference
species: 032843
name: (D)-Glucose-1,6-bisphosphate pool

032843
type: Species
substance units: 498143
is: SBO:0000247
is version of: CHEBI:17680
has only substance units: false
constant: false
in compartment: 032805
is version of: C00660
initial amount: NaN
initial concentration: 10000.0
boundary condition: false
name: (D)-Glucose-1,6-bisphosphate pool

498815
type: SpeciesReference
stoichiometry: 1.0
species: 032815
name: H+

498791
type: SpeciesReference
stoichiometry: 1.0
species: 032850
name: G6P pool

498803
type: SpeciesReference
stoichiometry: 1.0
species: 032838
name: ADP pool

498766
type: SpeciesReference
stoichiometry: 1.0
species: 032835
name: ATP pool

498839
type: ModifierSpeciesReference
species: 032842
name: Reduced glutathione

498778
type: SpeciesReference
stoichiometry: 1.0
species: 032830
name: Glucose

498827
type: ModifierSpeciesReference
species: CHK
name: Hexokinase (cytosolic)

033055
type: Parameter
is: SBO:0000025
constant: true
units: 498071
value: 0.0198
name: Isoamylase kcat

DPE2 KMQ
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 1100.0
name: DPE2 GlcAG KM

MEX KM
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 4000.0
name: Maltose exporter (MEX) KM

033060
type: Parameter
is: SBO:0000281
constant: true
units: dimensionless
value: 155.0
name: Hexokinase Keq

032930
type: Parameter
is: SBO:0000503
constant: true
units: dimensionless
value: 1667.0
name: Number of Glucosyl units in a Starch molecule

032973
type: Parameter
is: SBO:0000025
constant: true
units: 498071
value: 50.0
name: DPE2 kcat

DPE2 Keq
type: Parameter
is: SBO:0000281
constant: true
units: dimensionless
value: 1.0
name: DPE2 Keq

032943
type: Parameter
is: SBO:0000504
constant: true
units: 498375
value: 5.04E-4
name: Maltotriose Mol. Wt.

DPE1 KiA
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 746.42
name: DPE1 G3 Ki

DPE2 RXN
type: Reaction
is: SBO:0000559
modifier: 499008
reactant: 498972
fast: false
name: DPE2
reactant: 498960
product: 498996
reversible: true
product: 498984
kinetic law: 499021

499008
type: ModifierSpeciesReference
species: DPE2
name: DPE2

498972
type: SpeciesReference
stoichiometry: 1.0
species: 032831
name: AG

498960
type: SpeciesReference
stoichiometry: 1.0
species: 032819
name: Maltose

498996
type: SpeciesReference
stoichiometry: 1.0
species: 032834
name: GlcAG

498984
type: SpeciesReference
stoichiometry: 1.0
species: 032830
name: Glucose

ORG:
  orgname: National Renewable Energy Laboratory
n:
  given: Ambarish
  family: Nag

033043
type: Parameter
is: SBO:0000027
constant: true
units: 498302
value: 0.5
name: Beta Amylase KM for Starch

033048
type: Parameter
is: SBO:0000281
constant: true
units: 498302
value: 18800.0
name: Keq for Maltotetraose degradation

032929
type: Parameter
is: SBO:0000504
constant: true
units: 498375
value: 0.27
name: Starch Mol. Wt.

033086
type: Parameter
is: SBO:0000009
constant: true
units: 498168
value: 3000.0
name: Hexokinase Ki(glutathione)

G transport
type: Reaction
kinetic law: 498754
modifier: 498742
product: 498729
reactant: 498717
is: SBO:0000185
fast: false
reversible: false
name: Plastidic Glucose transporter

498742
type: ModifierSpeciesReference
species: pGlcT
name: Glucose transporter(pGlcT)

498729
type: SpeciesReference
stoichiometry: 1.0
species: 032830
name: Glucose

498717
type: SpeciesReference
stoichiometry: 1.0
species: 032827
name: Glucose

032807
type: Compartment
is version of: GO:0009707
is: SBO:0000290
constant: true
units: 498119
size: 1000.0
spatial dimensions: 2.0
name: Chloroplast Outer Membrane

032935
type: Parameter
is: SBO:0000540
constant: true
units: dimensionless
value: 0.13
name: Fraction of starch degraded to Maltotriose

033067
type: Parameter
is: SBO:0000027
constant: true
units: 498168
value: 47.0
name: Hexokinase KM(glucose)

032936
type: Parameter
is: SBO:0000565
constant: true
units: 498375
value: 1.0
name: Unit conversion factor to meet SBML standards

ORG:
  orgname: EMBL-EBI
n:
  given: Vijayalakshmi
  family: Chelliah

W3 CDTF: Mon Nov 01 00:00:00 GMT 2010