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type: SBMLModel
length unit: length
created:
  W3 CDTF: Thu Mar 31 05:12:08 BST 2011
notes: This is the model of the minmal 2 feedback switch described in the article: Synthetic conversion of a graded receptor signal into a tunable, reversible switch. Santhosh Palani and Casim A. Sarkar, 2011, Molecular Systems Biology 7:480; doi: 10.1038/msb.2011.13 The ability to engineer an all-or-none cellular response to a given signaling ligand is important in applications ranging from biosensing to tissue engineering. However, synthetic gene network switches have been limited in their applicability and tunability due to their reliance on specific components to function. Here, we present a strategy for reversible switch design that instead relies only on a robust, easily constructed network topology with two positive feedback loops and we apply the method to create highly ultrasensitive (nH420), bistable cellular responses to a synthetic ligand/receptor complex. Independent modulation of the two feedback strengths enables rational tuning and some decoupling of steady-state (ultrasensitivity, signal amplitude, switching threshold, and bistability) and kinetic (rates of system activation and deactivation) response properties. Our integrated computational and synthetic biology approach elucidates design rules for building cellular switches with desired properties, which may be of utility in engineering signal-transduction pathways. This model is parametrised for a transcription factor and receptor feedback strength of 3, TFs = 3 and Rs = 3. To reproduce figure 1 E, the parameters TFs and Rs have to be varied accordingly. Nomenclature for the model: L : Ligand R : Receptor C : Ligand-Receptor Complex I : Inactive Transcription Factor X : C bound to I A : Active Transcription Factor This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2011 The BioModels.net Team. For more information see the terms of use . To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, D…
creator:
  ORG:
    orgname: University of Pennsylvania
  n:
    given: Santhosh
    family: Palani
name: Palini2011_Minimal_2_Feedback_Model
parameter: kdegR meta
modified:
  W3 CDTF: Tue Nov 29 12:07:14 GMT 2011
occurs in: http://identifiers.org/taxonomy/4932
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parameter: kdegA meta
reaction: I degradation meta
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species: X meta
parameter: k2 meta
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is described by: http://identifiers.org/pubmed/21451590
creator:
  ORG:
    orgname: EMBL-EBI
  n:
    given: Lukas
    family: Endler
is: MODEL1102160000
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