BIOMD0000000247
type: SBMLModel
reaction: 574388
species: metaid F6P
reaction: metaid vSUC
is: MODEL1003300000
species: metaid Erythrose4P
species: metaid GLCo
reaction: metaid vENO
species: metaid PEP
reaction: metaid vALD
reaction: metaid vPFK
parameter: metaid SUMAXP
reaction: 574390
parameter: 574632
species: metaid G6P
species: metaid NAD
reaction: metaid vPGM
species: metaid P3G
species: metaid Ribulose5P
name: Ralser2007_Carbohydrate_Rerouting_ROS
species: metaid Xyl5P
has version: sce00030
has version: GO:0006739
units def: 905511
has version: sce00010
species: metaid GLCi
species: metaid NADH
compartment: metaid extracellular
reaction: 574386
is version of: GO:0034599
reaction: 574393
species: metaid NADPH
units def: 905463
reaction: metaid vPYK
reaction: metaid vATP
species: metaid Seduhept7P
curated: true
area units: metaid_0000005
species: metaid DHAP
species: metaid F26BP
reaction: metaid vPGI
species: metaid D6PGluconate
species: metaid D6PGluconoLactone
volume units: 845199
parameter: 574633
units def: 905439
has version: GO:0006096
species: metaid Ribose5P
reaction: 574389
reaction: 574391
units def: 905415
parameter: 574634
notes: This is the model with unfitted parameters described in the article Dynamic rerouting of the carbohydrate flux is key to counteracting oxidative stress Markus Ralser, Mirjam M Wamelink, Axel Kowald, Birgit Gerisch, Gino Heeren, Eduard A Struys, Edda Klipp, Cornelis Jakobs, Michael Breitenbach, Hans Lehrach and Sylvia Krobitsch, J Biol 2007 6(4):10; PMID: 18154684 , doi: 10.1186/jbiol61 Abstract: BACKGROUND: Eukaryotic cells have evolved various response mechanisms to counteract the deleterious consequences of oxidative stress. Among these processes, metabolic alterations seem to play an important role. RESULTS: We recently discovered that yeast cells with reduced activity of the key glycolytic enzyme triosephosphate isomerase exhibit an increased resistance to the thiol-oxidizing reagent diamide. Here we show that this phenotype is conserved in Caenorhabditis elegans and that the underlying mechanism is based on a redirection of the metabolic flux from glycolysis to the pentose phosphate pathway, altering the redox equilibrium of the cytoplasmic NADP(H) pool. Remarkably, another key glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), is known to be inactivated in response to various oxidant treatments, and we show that this provokes a similar redirection of the metabolic flux. CONCLUSION: The naturally occurring inactivation of GAPDH functions as a metabolic switch for rerouting the carbohydrate flux to counteract oxidative stress. As a consequence, altering the homoeostasis of cytoplasmic metabolites is a fundamental mechanism for balancing the redox state of eukaryotic cells under stress conditions. Different realtive enzyme velocities can be simulated by varying the parameters k_rel_TPI and k_rel_GAPDH . This model originates from BioModels Database: A Database of Annotated Published Models. It is copyright (c) 2005-2010 The BioModels Team. For more information see the terms of use . To cite BioModels Database, please use Le Novère N., Bornstein …
reaction: 574394
reaction: metaid vPGK
species: metaid P
species: metaid ETOH
length unit: length
reaction: metaid vG3PDH
species: metaid GA3P
species: metaid CO2
reaction: metaid vADH
reaction: 574384
reaction: metaid vGLT
reaction: metaid vGLK
species: metaid NADP
has version: GO:0006098
reaction: metaid vGAPDH
species: 574230
compartment: metaid cytoplasm
reaction: metaid vPDC
species: metaid SUCC
reaction: 574383
species: metaid PYR
substance units: 905415
species: metaid GLY
species: metaid P2G
occurs in: http://identifiers.org/taxonomy/4932
time units: 905439
species: metaid F16P

574388
type: Reaction
name: vPPI
product: 906485
fast: false
reactant: 906473
is: SBO:0000377
kinetic law: 906497
reversible: true
is version of: GO:0004751
is version of: R01056

906485
type: SpeciesReference
stoichiometry: 1.0
species: metaid Ribose5P

metaid Ribose5P
type: Species
is: CHEBI:17797
boundary condition: false
is: SBO:0000247
initial concentration: 0.1
in compartment: metaid cytoplasm
constant: false
initial amount: NaN
name: ribose 5-phosphate
has only substance units: false

metaid cytoplasm
type: Compartment
is: GO:0005829
constant: true
size: 1.0
spatial dimensions: 3.0
name: cytoplasm

906473
type: SpeciesReference
stoichiometry: 1.0
species: metaid Ribulose5P

metaid Ribulose5P
type: Species
name: ribulose 5-phosphate
has only substance units: false
initial amount: NaN
is: SBO:0000247
is: CHEBI:17363
constant: false
initial concentration: 0.1
in compartment: metaid cytoplasm
boundary condition: false

574594
type: LocalParameter
units: 905511
value: 1.6
name: KmRibo5P

905511
type: SBMLUnitsDef
unit: 905535
unit: 905523
name: mM

905535
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: LITRE

905523
type: SBMLUnit
exponent: 1.0
scale: -3
multipler: 1.0
kind: MOLE

574593
type: LocalParameter
units: 905511
value: 1.6
name: KmRibu5P

574592
type: LocalParameter
units: 905463
value: 3458.0
name: VmPPIr

905463
type: SBMLUnitsDef
unit: 905499
unit: 905487
unit: 905475
name: mMpermin

905499
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 60.0
kind: SECOND

905487
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 1.0
kind: LITRE

905475
type: SBMLUnit
exponent: 1.0
scale: -3
multipler: 1.0
kind: MOLE

574590
type: LocalParameter
units: 905463
value: 3458.0
name: VmPPIf

metaid F6P
type: Species
has only substance units: false
initial concentration: 0.28
boundary condition: false
initial amount: NaN
is: SBO:0000247
in compartment: metaid cytoplasm
is: CHEBI:15946
constant: false
name: Fructose 6 Phosphate
is: C05345

metaid vSUC
type: Reaction
reactant: 906100
is version of: GO:0006105
reversible: true
reactant: 906112
name: Succinate synthesis
product: 906136
product: 906124
is: SBO:0000205
kinetic law: 906148
fast: false

906100
type: SpeciesReference
stoichiometry: 2.0
species: metaid ACE

metaid ACE
type: Species
constant: false
name: Acetaldehyde
in compartment: metaid cytoplasm
initial concentration: 0.04
is: CHEBI:15343
initial amount: NaN
has only substance units: false
is: SBO:0000247
boundary condition: false
is: C00084

906112
type: SpeciesReference
stoichiometry: 3.0
species: metaid NAD

metaid NAD
type: Species
constant: false
is: CHEBI:15846
initial concentration: 1.2
initial amount: NaN
has only substance units: false
boundary condition: false
in compartment: metaid cytoplasm
is: SBO:0000247
is: C00003
name: NAD

906136
type: SpeciesReference
stoichiometry: 1.0
species: metaid SUCC

metaid SUCC
type: Species
is: SBO:0000247
constant: false
boundary condition: true
name: Succinate
has only substance units: false
is: CHEBI:30031
initial concentration: 0.1
initial amount: NaN
in compartment: metaid extracellular

metaid extracellular
type: Compartment
is: GO:0005576
is: SBO:0000290
constant: true
size: 1.0
spatial dimensions: 3.0
name: extracellular

906124
type: SpeciesReference
stoichiometry: 3.0
species: metaid NADH

metaid NADH
type: Species
is: C00004
initial concentration: 0.39
in compartment: metaid cytoplasm
is: CHEBI:16908
has only substance units: false
is: SBO:0000247
constant: false
initial amount: NaN
name: NADH
boundary condition: false

574556
type: LocalParameter
units: 905548
value: 21.4

905548
type: SBMLUnitsDef
unit: 905560
name: permin

905560
type: SBMLUnit
exponent: -1.0
scale: 0
multipler: 60.0
kind: SECOND

metaid Erythrose4P
type: Species
has only substance units: false
constant: false
name: erythrose 4-phosphate
is: SBO:0000247
initial amount: NaN
in compartment: metaid cytoplasm
is: CHEBI:48153
boundary condition: false
initial concentration: 0.0

metaid GLCo
type: Species
boundary condition: true
has only substance units: false
in compartment: metaid extracellular
initial amount: NaN
is: C00293
initial concentration: 50.0
constant: false
is: SBO:0000247
is: CHEBI:17234
name: Extracellular Glucose

metaid vENO
type: Reaction
is: R00658
is: SBO:0000211
reactant: 905968
kinetic law: 905992
product: 905980
reversible: true
fast: false
is: http://identifiers.org/ec-code/4.2.1.11
is homolog to: REACT 1400
name: Enolase

905968
type: SpeciesReference
stoichiometry: 1.0
species: metaid P2G

metaid P2G
type: Species
is: SBO:0000247
boundary condition: false
name: 2-phosphoglycerate
initial amount: NaN
in compartment: metaid cytoplasm
has only substance units: false
is: CHEBI:17835
constant: false
initial concentration: 0.1
is: C00631

574544
type: LocalParameter
units: 905511
value: 0.5

574543
type: LocalParameter
units: dimensionless
value: 6.7

574542
type: LocalParameter
units: 905511
value: 0.04

574540
type: LocalParameter
units: 905463
value: 365.806

905980
type: SpeciesReference
stoichiometry: 1.0
species: metaid PEP

metaid PEP
type: Species
boundary condition: false
initial amount: NaN
constant: false
name: Phosphoenolpyruvate
is: CHEBI:18021
has only substance units: false
initial concentration: 0.1
in compartment: metaid cytoplasm
is: C00074
is: SBO:0000247

metaid vALD
type: Reaction
product: 905740
is homolog to: REACT 1602
reactant: 905716
is: SBO:0000182
fast: false
product: 905728
is: http://identifiers.org/ec-code/4.1.2.13
kinetic law: 905752
reversible: true
is: R01070
name: Aldolase

905740
type: SpeciesReference
stoichiometry: 1.0
species: metaid GA3P

metaid GA3P
type: Species
has only substance units: false
constant: false
name: glyceraldehyde 3-phosphate
is: CHEBI:17138
is: SBO:0000247
initial concentration: 0.05
boundary condition: false
in compartment: metaid cytoplasm
initial amount: NaN

905716
type: SpeciesReference
stoichiometry: 1.0
species: metaid F16P

metaid F16P
type: Species
in compartment: metaid cytoplasm
boundary condition: false
initial amount: NaN
constant: false
is: CHEBI:16905
is: C00354
has only substance units: false
initial concentration: 0.1
is: SBO:0000247
name: Fructose-1,6 bisphosphate

905728
type: SpeciesReference
stoichiometry: 1.0
species: metaid DHAP

metaid DHAP
type: Species
name: dihydroxyacetone phosphate
constant: false
in compartment: metaid cytoplasm
initial amount: NaN
is: SBO:0000247
is: CHEBI:16108
has only substance units: false
initial concentration: 1.0
boundary condition: false

574504
type: LocalParameter
units: 905511
value: 10.0

574503
type: LocalParameter
units: 905511
value: 2.0

574502
type: LocalParameter
units: 905511
value: 2.4

574501
type: LocalParameter
units: 905511
value: 0.3

574499
type: LocalParameter
units: dimensionless
value: 0.069

574498
type: LocalParameter
units: dimensionless
value: 0.045

574497
type: LocalParameter
units: 905463
value: 322.258

metaid vPFK
type: Reaction
kinetic law: 905704
reactant: 905668
product: 905680
is: SBO:0000216
is: http://identifiers.org/ec-code/2.7.1.11
reactant: 905656
modifier: 905692
reversible: true
name: Phosphofructokinase
is: R00756
is homolog to: REACT 736
fast: false

574479
type: LocalParameter
units: dimensionless
value: 5.12

574493
type: LocalParameter
units: 905511
value: 0.0995

574488
type: LocalParameter
units: 905511
value: 6.82E-4

574496
type: LocalParameter
units: dimensionless
value: 3.0

574495
type: LocalParameter
units: 905511
value: 0.65

574489
type: LocalParameter
units: dimensionless
value: 0.397

574481
type: LocalParameter
units: dimensionless
value: 0.45

574491
type: LocalParameter
units: dimensionless
value: 0.0845

574486
type: LocalParameter
units: dimensionless
value: 0.0174

574494
type: LocalParameter
units: dimensionless
value: 100.0

574490
type: LocalParameter
units: 905511
value: 0.111

574485
type: LocalParameter
units: dimensionless
value: 0.66

574484
type: LocalParameter
units: 905511
value: 0.71

574480
type: LocalParameter
units: 905463
value: 182.903

574483
type: LocalParameter
units: 905511
value: 0.1

905668
type: SpeciesReference
stoichiometry: 1.0
species: metaid P

metaid P
type: Species
has only substance units: false
initial amount: NaN
in compartment: metaid cytoplasm
has part: C00008
initial concentration: 5.0
name: High energy phosphates
has part: CHEBI:15422
is: SBO:0000247
has part: CHEBI:16761
has part: C00002
constant: false
boundary condition: false

905680
type: SpeciesReference
stoichiometry: 1.0
species: metaid F16P

905656
type: SpeciesReference
stoichiometry: 1.0
species: metaid F6P

metaid F26BP
type: Species
in compartment: metaid cytoplasm
boundary condition: true
initial amount: NaN
is: CHEBI:28602
is: SBO:0000247
is: C00665
has only substance units: false
initial concentration: 0.02
name: F2,6P
constant: false

metaid SUMAXP
type: Parameter
has part: CHEBI:16761
has part: C00008
has part: CHEBI:15422
name: sum of AXP conc
value: 4.1
has part: CHEBI:16027
constant: true
has part: C00020
has part: C00002

574390
type: Reaction
kinetic law: 906594
product: 906582
reactant: 906570
is: SBO:0000377
fast: false
reversible: true
name: vR5PI

574607
type: LocalParameter
units: 905511
value: 1.5
name: KmXyl

574606
type: LocalParameter
units: 905511
value: 1.5
name: KmRibu5P

574605
type: LocalParameter
units: 905463
value: 1039.0
name: VmR5PIr

574603
type: LocalParameter
units: 905463
value: 1039.0
name: VmR5PIf

906582
type: SpeciesReference
stoichiometry: 1.0
species: metaid Xyl5P

metaid Xyl5P
type: Species
in compartment: metaid cytoplasm
initial amount: NaN
constant: false
is: CHEBI:16332
initial concentration: 0.1
boundary condition: false
is: SBO:0000247
name: xylulose 5-phosphate
has only substance units: false

906570
type: SpeciesReference
stoichiometry: 1.0
species: metaid Ribulose5P

574632
type: Parameter
constant: true
units: dimensionless
value: 1.0
name: k_rel_TPI

metaid G6P
type: Species
name: Glucose 6 Phosphate
initial amount: NaN
boundary condition: false
in compartment: metaid cytoplasm
constant: false
has only substance units: false
initial concentration: 1.39
is: C00668
is: CHEBI:17665
is: SBO:0000247

metaid vPGM
type: Reaction
is: R01518
name: Phosphoglycerate mutase
kinetic law: 905956
is: SBO:0000377
product: 905944
is homolog to: REACT 576
is: http://identifiers.org/ec-code/5.4.2.1
reversible: true
reactant: 905932
fast: false

574538
type: LocalParameter
units: 905511
value: 0.08

574537
type: LocalParameter
units: dimensionless
value: 0.19

574535
type: LocalParameter
units: 905511
value: 1.2

574534
type: LocalParameter
units: 905463
value: 2525.81

905944
type: SpeciesReference
stoichiometry: 1.0
species: metaid P2G

905932
type: SpeciesReference
stoichiometry: 1.0
species: metaid P3G

metaid P3G
type: Species
initial concentration: 0.1
initial amount: NaN
has only substance units: false
is: CHEBI:17794
in compartment: metaid cytoplasm
is: SBO:0000247
name: 3-phosphoglycerate
is: C00197
boundary condition: false
constant: false

metaid GLCi
type: Species
in compartment: metaid cytoplasm
initial concentration: 0.087
name: Glucose in Cytosol
is: SBO:0000247
constant: false
initial amount: NaN
is: C00293
is: CHEBI:17234
boundary condition: false
has only substance units: false

574386
type: Reaction
fast: false
name: vGluDH
is version of: R01528
product: 906437
is version of: GO:0004616
reactant: 906413
is: SBO:0000200
reversible: false
product: 906425
modifier: 906449
kinetic law: 906461
reactant: 906400

906437
type: SpeciesReference
stoichiometry: 1.0
species: metaid NADPH

metaid NADPH
type: Species
is: CHEBI:16474
initial concentration: 1.6
initial amount: NaN
in compartment: metaid cytoplasm
boundary condition: false
has only substance units: false
name: NADPH
is: SBO:0000247
constant: false

906413
type: SpeciesReference
stoichiometry: 1.0
species: metaid NADP

metaid NADP
type: Species
has only substance units: false
boundary condition: false
is: CHEBI:18009
initial amount: NaN
constant: false
in compartment: metaid cytoplasm
name: NADP+
is: SBO:0000247
initial concentration: 0.4

906425
type: SpeciesReference
stoichiometry: 1.0
species: metaid Ribulose5P

574588
type: LocalParameter
units: 905511
value: 0.03
name: KiNADPH

574586
type: LocalParameter
units: 905511
value: 0.03
name: KmNADP

574585
type: LocalParameter
units: 905511
value: 0.02
name: KmGluconate

574584
type: LocalParameter
units: 905463
value: 4.0
name: VmGluDH

906400
type: SpeciesReference
stoichiometry: 1.0
species: metaid D6PGluconate

metaid D6PGluconate
type: Species
boundary condition: false
in compartment: metaid cytoplasm
name: 6-phosphogluconate
constant: false
has only substance units: false
is: CHEBI:16863
initial amount: NaN
initial concentration: 0.1
is: SBO:0000247

574393
type: Reaction
reactant: 906678
fast: false
name: vTransk2
reactant: 906666
is version of: GO:0004802
product: 906690
reversible: true
kinetic law: 906714
product: 906702
is: SBO:0000182
is version of: R01830

906678
type: SpeciesReference
stoichiometry: 1.0
species: metaid Erythrose4P

906666
type: SpeciesReference
stoichiometry: 1.0
species: metaid Xyl5P

906690
type: SpeciesReference
stoichiometry: 1.0
species: metaid GA3P

574621
type: LocalParameter
units: 905511
value: 2.1
name: KmGA3P

574620
type: LocalParameter
units: 905511
value: 1.1
name: KmF6P

574619
type: LocalParameter
units: 905511
value: 0.09
name: KmEry4P

574617
type: LocalParameter
units: 905511
value: 0.16
name: KmXyl5P

574616
type: LocalParameter
units: 905463
value: 43.0
name: VmTransk2r

574615
type: LocalParameter
units: 905463
value: 3.2
name: VmTransk2F

906702
type: SpeciesReference
stoichiometry: 1.0
species: metaid F6P

metaid vPYK
type: Reaction
is: http://identifiers.org/ec-code/2.7.1.40
is: R00200
product: 906016
is: SBO:0000216
reversible: true
fast: false
is homolog to: REACT 1911
reactant: 906004
product: 906028
name: Pyruvate kinase
kinetic law: 906040

906016
type: SpeciesReference
stoichiometry: 1.0
species: metaid PYR

metaid PYR
type: Species
name: Pyruvate
initial concentration: 3.36
constant: false
is: C00022
initial amount: NaN
is: SBO:0000247
boundary condition: false
in compartment: metaid cytoplasm
has only substance units: false
is: CHEBI:15361

906004
type: SpeciesReference
stoichiometry: 1.0
species: metaid PEP

906028
type: SpeciesReference
stoichiometry: 1.0
species: metaid P

574552
type: LocalParameter
units: 905511
value: 1.5

574551
type: LocalParameter
units: 905511
value: 21.0

574550
type: LocalParameter
units: dimensionless
value: 6500.0

574548
type: LocalParameter
units: dimensionless
value: 0.45

574547
type: LocalParameter
units: 905511
value: 0.53

574546
type: LocalParameter
units: 905511
value: 0.14

574545
type: LocalParameter
units: 905463
value: 1088.71

metaid vATP
type: Reaction
kinetic law: 906244
is version of: GO:0016887
reversible: true
is: SBO:0000376
reactant: 906220
is version of: http://identifiers.org/ec-code/3.6.1.3
product: 906232
name: ATPase activity
fast: false

574571
type: LocalParameter
units: dimensionless
value: 0.45

574570
type: LocalParameter
units: 905548
value: 39.5

906220
type: SpeciesReference
stoichiometry: 1.0
species: metaid P

906232
type: SpeciesReference
stoichiometry: 1.0
species: 574230

574230
type: Species
is: SBO:0000291
constant: false
boundary condition: true
has only substance units: false
initial concentration: 0.1
initial amount: NaN
in compartment: metaid cytoplasm
name: X

metaid Seduhept7P
type: Species
initial concentration: 0.1
name: sedoheptulose 7-phosphate
initial amount: NaN
boundary condition: false
constant: false
is: CHEBI:15721
is: SBO:0000247
in compartment: metaid cytoplasm
has only substance units: false

metaid vPGI
type: Reaction
product: 905632
reversible: true
is: SBO:0000377
reactant: 905620
name: Glucose-6-phosphate isomerase
is: R00771
fast: false
is: http://identifiers.org/ec-code/5.3.1.9
kinetic law: 905644
is homolog to: REACT 116

905632
type: SpeciesReference
stoichiometry: 1.0
species: metaid F6P

905620
type: SpeciesReference
stoichiometry: 1.0
species: metaid G6P

574477
type: LocalParameter
units: 905511
value: 0.3

574446
type: LocalParameter
units: dimensionless
value: 0.314

574445
type: LocalParameter
units: 905511
value: 1.4

574444
type: LocalParameter
units: 905463
value: 339.677

metaid D6PGluconoLactone
type: Species
boundary condition: false
in compartment: metaid cytoplasm
is: SBO:0000247
has only substance units: false
is: CHEBI:16938
initial amount: NaN
initial concentration: 0.1
constant: false
name: D-6-phosphoglucono-delta-lactone

574633
type: Parameter
constant: true
units: dimensionless
value: 1.0
name: k_rel_GAPDH

905439
type: SBMLUnitsDef
unit: 905451
name: minute

905451
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 60.0
kind: SECOND

574389
type: Reaction
kinetic law: 906558
is version of: R01641
product: 906534
product: 906546
is version of: GO:0004802
reversible: true
name: vTransk1
fast: false
reactant: 906522
reactant: 906509
is: SBO:0000182

574601
type: LocalParameter
units: 905511
value: 0.15
name: KmSeduhept

574600
type: LocalParameter
units: 905511
value: 0.1
name: KmGA3P

574598
type: LocalParameter
units: 905511
value: 0.15
name: KmXyl5P

574597
type: LocalParameter
units: 905511
value: 0.1
name: KmRibose5P

574596
type: LocalParameter
units: 905463
value: 2.0
name: VmTransk1r

574595
type: LocalParameter
units: 905463
value: 4.0
name: VmTransk1f

906534
type: SpeciesReference
stoichiometry: 1.0
species: metaid GA3P

906546
type: SpeciesReference
stoichiometry: 1.0
species: metaid Seduhept7P

906522
type: SpeciesReference
stoichiometry: 1.0
species: metaid Xyl5P

906509
type: SpeciesReference
stoichiometry: 1.0
species: metaid Ribose5P

574391
type: Reaction
fast: false
product: 906642
reactant: 906606
is version of: GO:0004801
reversible: true
kinetic law: 906654
is version of: R01827
product: 906630
is: SBO:0000182
name: vTransald
reactant: 906618

906642
type: SpeciesReference
stoichiometry: 1.0
species: metaid Erythrose4P

906606
type: SpeciesReference
stoichiometry: 1.0
species: metaid Seduhept7P

574614
type: LocalParameter
units: 905511
value: 0.018
name: KmEry4P

574613
type: LocalParameter
units: 905511
value: 0.32
name: KmF6P

574612
type: LocalParameter
units: 905511
value: 0.18
name: KmSeduhept

574610
type: LocalParameter
units: 905511
value: 0.22
name: KmGA3P

574609
type: LocalParameter
units: 905463
value: 10.0
name: VmTransaldr

574608
type: LocalParameter
units: 905463
value: 55.0
name: VmTransaldf

906630
type: SpeciesReference
stoichiometry: 1.0
species: metaid F6P

906618
type: SpeciesReference
stoichiometry: 1.0
species: metaid GA3P

905415
type: SBMLUnitsDef
unit: 905427
name: millimole

905427
type: SBMLUnit
exponent: 1.0
scale: -3
multipler: 1.0
kind: MOLE

574634
type: Parameter
constant: false
units: dimensionless
value: NaN
name: ratio_NADPH_NADP

574394
type: Reaction
kinetic law: 906751
product: 906738
reactant: 906726
is version of: GO:0070995
is: SBO:0000201
fast: false
reversible: false
name: vNADPH

574622
type: LocalParameter
units: 905548
value: 2.0
name: kNADPH

906738
type: SpeciesReference
stoichiometry: 1.0
species: metaid NADP

906726
type: SpeciesReference
stoichiometry: 1.0
species: metaid NADPH

metaid vPGK
type: Reaction
product: 905896
is homolog to: REACT 1771
reactant: 905884
is: SBO:0000216
product: 905908
reversible: true
kinetic law: 905920
name: Phosphoglycerate kinase
is: http://identifiers.org/ec-code/2.7.2.3
is: R01512
fast: false

905896
type: SpeciesReference
stoichiometry: 1.0
species: metaid P3G

905884
type: SpeciesReference
stoichiometry: 1.0
species: metaid BPG

metaid BPG
type: Species
constant: false
initial amount: NaN
is: SBO:0000247
initial concentration: 0.1
is: C00236
is: CHEBI:16001
boundary condition: false
has only substance units: false
name: 1,3-bisphosphoglycerate
in compartment: metaid cytoplasm

905908
type: SpeciesReference
stoichiometry: 1.0
species: metaid P

574532
type: LocalParameter
units: 905511
value: 0.003

574530
type: LocalParameter
units: 905511
value: 0.2

574529
type: LocalParameter
units: 905511
value: 0.53

574528
type: LocalParameter
units: 905511
value: 0.3

574527
type: LocalParameter
units: dimensionless
value: 0.45

574525
type: LocalParameter
units: dimensionless
value: 3200.0

574524
type: LocalParameter
units: 905463
value: 1306.45

metaid ETOH
type: Species
initial amount: NaN
initial concentration: 50.0
is: SBO:0000247
name: Ethanol
is: C00469
constant: false
boundary condition: true
is: CHEBI:16236
has only substance units: false
in compartment: metaid extracellular

metaid vG3PDH
type: Reaction
reactant: 905764
reactant: 905776
fast: false
is: SBO:0000200
product: 905800
name: Glycerol 3-phosphate dehydrogenase
is version of: http://identifiers.org/ec-code/1.1.1.8
product: 905788
kinetic law: 905812
reversible: true

905764
type: SpeciesReference
stoichiometry: 1.0
species: metaid DHAP

905776
type: SpeciesReference
stoichiometry: 1.0
species: metaid NADH

905800
type: SpeciesReference
stoichiometry: 1.0
species: metaid NAD

905788
type: SpeciesReference
stoichiometry: 1.0
species: metaid GLY

metaid GLY
type: Species
is: SBO:0000247
is: C00116
boundary condition: true
constant: false
initial amount: NaN
is: CHEBI:17754
has only substance units: false
in compartment: metaid extracellular
name: Glycerol
initial concentration: 0.15

574512
type: LocalParameter
units: 905511
value: 1.0

574511
type: LocalParameter
units: 905511
value: 0.93

574510
type: LocalParameter
units: 905511
value: 0.023

574509
type: LocalParameter
units: 905511
value: 0.4

574507
type: LocalParameter
units: dimensionless
value: 0.045

574506
type: LocalParameter
units: dimensionless
value: 4300.0

574505
type: LocalParameter
units: 905463
value: 70.15

metaid CO2
type: Species
constant: false
boundary condition: true
initial amount: NaN
initial concentration: 1.0
is: SBO:0000247
has only substance units: false
is: CHEBI:16526
is: C00011
name: CO2
in compartment: metaid extracellular

metaid vADH
type: Reaction
reactant: 906172
is version of: R00746
is: SBO:0000200
name: Alcohol dehydrogenase
is homolog to: REACT 799
is version of: http://identifiers.org/ec-code/1.1.1.2
kinetic law: 906208
reversible: true
reactant: 906160
fast: false
product: 906196
product: 906184

906172
type: SpeciesReference
stoichiometry: 1.0
species: metaid NADH

574558
type: LocalParameter
units: 905463
value: 810.0

574567
type: LocalParameter
units: 905511
value: 1.11

574564
type: LocalParameter
units: 905511
value: 0.11

574562
type: LocalParameter
units: dimensionless
value: 6.9E-5

574559
type: LocalParameter
units: 905511
value: 0.92

574561
type: LocalParameter
units: 905511
value: 17.0

574563
type: LocalParameter
units: 905511
value: 0.17

574568
type: LocalParameter
units: 905511
value: 1.1

574566
type: LocalParameter
units: 905511
value: 0.031

574569
type: LocalParameter
units: 905511
value: 90.0

906160
type: SpeciesReference
stoichiometry: 1.0
species: metaid ACE

906196
type: SpeciesReference
stoichiometry: 1.0
species: metaid NAD

906184
type: SpeciesReference
stoichiometry: 1.0
species: metaid ETOH

574384
type: Reaction
fast: false
name: vG6PDH
product: 906328
is version of: R00835
reactant: 906292
kinetic law: 906352
reversible: false
modifier: 906340
is version of: http://identifiers.org/ec-code/1.1.1.49
is version of: GO:0004345
product: 906316
reactant: 906304
is: SBO:0000200

906328
type: SpeciesReference
stoichiometry: 1.0
species: metaid NADPH

906292
type: SpeciesReference
stoichiometry: 1.0
species: metaid G6P

574580
type: LocalParameter
units: 905511
value: 0.017
name: KiNADPH

574578
type: LocalParameter
units: 905511
value: 0.02
name: KmNADP

574577
type: LocalParameter
units: 905511
value: 0.04
name: KmG6P

574576
type: LocalParameter
units: 905463
value: 4.0
name: VmG6PDH

906316
type: SpeciesReference
stoichiometry: 1.0
species: metaid D6PGluconoLactone

906304
type: SpeciesReference
stoichiometry: 1.0
species: metaid NADP

metaid vGLT
type: Reaction
kinetic law: 906788
fast: false
name: Glucose transport
is: SBO:0000185
reactant: 906763
reversible: true
is version of: GO:0046323
product: 906776
is homolog to: REACT 2092

574628
type: LocalParameter
units: 905511
value: 1.1918
name: KmGLTGLCi

574627
type: LocalParameter
units: 905511
value: 1.1918
name: KmGLTGLCo

574626
type: LocalParameter
units: 905511
value: 1.0
name: KeqGLT

574624
type: LocalParameter
units: 905463
value: 97.264
name: VmGLT

906763
type: SpeciesReference
stoichiometry: 1.0
species: metaid GLCo

906776
type: SpeciesReference
stoichiometry: 1.0
species: metaid GLCi

metaid vGLK
type: Reaction
kinetic law: 905608
name: Hexokinase
reactant: 905584
is homolog to: REACT 1318
reactant: 905572
fast: false
reversible: true
product: 905596
is: R00299
is: http://identifiers.org/ec-code/2.7.1.2
is: SBO:0000216

574431
type: LocalParameter
units: 905511
value: 0.23

574408
type: LocalParameter
units: 905511
value: 30.0

574406
type: LocalParameter
units: 905511
value: 0.08

574405
type: LocalParameter
units: 905511
value: 0.15

574404
type: LocalParameter
units: dimensionless
value: 3800.0

574403
type: LocalParameter
units: dimensionless
value: 0.45

574401
type: LocalParameter
units: 905463
value: 226.452

905584
type: SpeciesReference
stoichiometry: 1.0
species: metaid P

905572
type: SpeciesReference
stoichiometry: 1.0
species: metaid GLCi

905596
type: SpeciesReference
stoichiometry: 1.0
species: metaid G6P

metaid vGAPDH
type: Reaction
is: http://identifiers.org/ec-code/1.2.1.12
reactant: 905836
fast: false
is homolog to: REACT 1847
product: 905860
product: 905848
is: SBO:0000200
reactant: 905824
name: Glyceraldehyde 3-phosphate dehydrogenase
kinetic law: 905872
reversible: true
is: R01061

905836
type: SpeciesReference
stoichiometry: 1.0
species: metaid NAD

905860
type: SpeciesReference
stoichiometry: 1.0
species: metaid NADH

905848
type: SpeciesReference
stoichiometry: 1.0
species: metaid BPG

905824
type: SpeciesReference
stoichiometry: 1.0
species: metaid GA3P

574522
type: LocalParameter
units: dimensionless
value: 0.005
name: KeqGAPDH

574520
type: LocalParameter
units: 905511
value: 0.09

574519
type: LocalParameter
units: 905511
value: 0.21

574518
type: LocalParameter
units: 905463
value: 1184.52

574517
type: LocalParameter
units: dimensionless
value: 0.045

574515
type: LocalParameter
units: 905511
value: 0.06

574514
type: LocalParameter
units: 905511
value: 0.0098

574513
type: LocalParameter
units: 905463
value: 6549.68

metaid vPDC
type: Reaction
product: 906076
kinetic law: 906088
is: R00224
fast: false
reactant: 906052
product: 906064
name: Pyruvate decarboxylase
is: SBO:0000399
is: http://identifiers.org/ec-code/4.1.1.1
reversible: true

906076
type: SpeciesReference
stoichiometry: 1.0
species: metaid CO2

574555
type: LocalParameter
units: 905511
value: 4.33

574554
type: LocalParameter
units: dimensionless
value: 1.9

574553
type: LocalParameter
units: 905463
value: 174.194

906052
type: SpeciesReference
stoichiometry: 1.0
species: metaid PYR

906064
type: SpeciesReference
stoichiometry: 1.0
species: metaid ACE

574383
type: Reaction
reversible: true
reactant: 906256
kinetic law: 906280
name: vTPI
is version of: R01015
is version of: GO:0004807
product: 906268
is version of: http://identifiers.org/ec-code/5.3.1.1
is: SBO:0000377
fast: false

906256
type: SpeciesReference
stoichiometry: 1.0
species: metaid GA3P

574575
type: LocalParameter
units: 905463
value: 555.0
name: VmGA3P

574574
type: LocalParameter
units: 905463
value: 10900.0
name: VmDHAP

574573
type: LocalParameter
units: 905511
value: 1.23
name: KmDHAP

574572
type: LocalParameter
units: 905511
value: 1.27
name: KmGA3P

906268
type: SpeciesReference
stoichiometry: 1.0
species: metaid DHAP

574385
type: Reaction
is version of: R02035
fast: false
is version of: GO:0017057
reactant: 906364
name: v6PGL
is: SBO:0000376
kinetic law: 906388
reversible: false
product: 906376

906364
type: SpeciesReference
stoichiometry: 1.0
species: metaid D6PGluconoLactone

574583
type: LocalParameter
units: 905511
value: 0.8
name: Km6PGL

574582
type: LocalParameter
units: 905463
value: 4.0
name: Vm6PGL

906376
type: SpeciesReference
stoichiometry: 1.0
species: metaid D6PGluconate

W3 CDTF: Wed Apr 11 12:09:00 BST 2007

W3 CDTF: Tue May 15 22:59:06 BST 2012

ORG:
  orgname: Stellenbosh University
n:
  given: Jacky L
  family: Snoep

ORG:
  orgname: University of Manchester
n:
  given: Kieran
  family: Smallbone