BIOMD0000000211
type: SBMLModel
parameter: metaid_0000070
reaction: vGT
reaction: vPGT
reaction: vPFK
species: species 12
parameter: metaid_0000071
name: Rate Law for atp utilisation
species: species 8
reaction: vPT
time units: metaid_0000067
species: species 21
species: species 22
name: Rate Law for pyruvate kinase
name: Henri-Michaelis-Menten (irreversible)
species: species 15
species: species 7
reaction: vGPDH
is: MODEL1511155915
compartment: compartment 2
reaction: vPGI
name: Rate Law for aldolase
area units: metaid_0000005
species: species 4
function def: metaid_0000056
name: Reversible Michaelis-Menten
name: Rate Law for hexokinase
function def: metaid_0000060
is version of: GO:0006096
species: species 25
species: species 11
species: species 3
length unit: length
species: species 26
reaction: vAU
parameter: metaid_0000072
notes: This model is from the article: Experimental and in silico analyses of glycolytic flux control in bloodstream form Trypanosoma brucei. Albert MA, Haanstra JR, Hannaert V, Van Roy J, Opperdoes FR, Bakker BM, Michels PA. J Biol Chem 2005 Aug 5;280(31):28306-15. 15955817 , Abstract: A mathematical model of glycolysis in bloodstream form Trypanosoma brucei was developed previously on the basis of all available enzyme kinetic data (Bakker, B. M., Michels, P. A. M., Opperdoes, F. R., and Westerhoff, H. V. (1997) J. Biol. Chem. 272, 3207-3215). The model predicted correctly the fluxes and cellular metabolite concentrations as measured in non-growing trypanosomes and the major contribution to the flux control exerted by the plasma membrane glucose transporter. Surprisingly, a large overcapacity was predicted for hexokinase (HXK), phosphofructokinase (PFK), and pyruvate kinase (PYK). Here, we present our further analysis of the control of glycolytic flux in bloodstream form T. brucei. First, the model was optimized and extended with recent information about the kinetics of enzymes and their activities as measured in lysates of in vitro cultured growing trypanosomes. Second, the concentrations of five glycolytic enzymes (HXK, PFK, phosphoglycerate mutase, enolase, and PYK) in trypanosomes were changed by RNA interference. The effects of the knockdown of these enzymes on the growth, activities, and levels of various enzymes and glycolytic flux were studied and compared with model predictions. Data thus obtained support the conclusion from the in silico analysis that HXK, PFK, and PYK are in excess, albeit less than predicted. Interestingly, depletion of PFK and enolase had an effect on the activity (but not, or to a lesser extent, expression) of some other glycolytic enzymes. Enzymes located both in the glycosomes (the peroxisome-like organelles harboring the first seven enzymes of the glycolytic pathway of trypanosomes) and in the cytosol were affected. These data suggest the…
reaction: vGlyT
reaction: vPK
species: species 10
parameter: metaid_0000073
reaction: vAKg
species: species 18
species: species 6
species: species 19
name: Rate Law for phosphoglycerate kinase
units def: metaid_0000067
reaction: vANTI
name: Rate Law for glyceraldehyde3phosphatedehydrogenase
occurs in: http://identifiers.org/taxonomy/5691
is: tbr00010
species: species 20
function def: metaid_0000064
species: species 27
name: Albert2005_Glycolysis
compartment: compartment 3
name: Rate Law for adenylate kinase
function def: metaid_0000062
function def: metaid_0000061
function def: metaid_0000057
curated: true
is homolog to: REACT 1383
species: species 14
species: species 2
reaction: vPGM
name: Rate Law for phophofructokinase
reaction: vTPI
reaction: vENO
name: Rate Law for glucose transport
reaction: vGPO
units def: metaid_0000068
species: species 23
species: species 1
species: species 24
reaction: vGK
species: species 17
species: species 9
reaction: vHK
substance units: metaid_0000068
parameter: metaid_0000074

metaid_0000070
type: Parameter
constant: true
value: 1.0
name: RaHXK

vGT
type: Reaction
kinetic law: 671233
product: 671221
reactant: 671209
is version of: GO:0046323
is homolog to: REACT 2092
fast: false
reversible: true
name: glucose transport

047944
type: LocalParameter
value: 0.75

047942
type: LocalParameter
value: 1.0

047940
type: LocalParameter
value: 108.9

671221
type: SpeciesReference
stoichiometry: 1.0
species: species 10

species 10
type: Species
name: glucose
is: CHEBI:4167
initial amount: NaN
is: C00031
in compartment: compartment 2
is: http://identifiers.org/pubchem.substance/3587
has only substance units: false
constant: false
initial concentration: 0.0
boundary condition: false

compartment 2
type: Compartment
is: GO:0020015
constant: true
size: 1.0
spatial dimensions: 3.0
name: glycosome

671209
type: SpeciesReference
stoichiometry: 1.0
species: species 25

species 25
type: Species
initial amount: NaN
is: C00031
constant: true
initial concentration: 5.0
name: glucose external
boundary condition: true
in compartment: compartment 3
has only substance units: false
is: CHEBI:4167

compartment 3
type: Compartment
is: GO:0005576
constant: true
size: 1.0
spatial dimensions: 3.0
name: extracellular

vPGT
type: Reaction
kinetic law: 672125
product: 672113
reactant: 672101
is version of: GO:0015120
fast: false
reversible: true
name: 3phosphoglycerate transport

048082
type: LocalParameter
value: 1000000.0

048080
type: LocalParameter
value: 1000000.0

672113
type: SpeciesReference
stoichiometry: 1.0
species: species 7

species 7
type: Species
is: C00197
in compartment: compartment 1
is: http://identifiers.org/pubchem.substance/3497
initial amount: NaN
boundary condition: false
is: CHEBI:17794
has only substance units: false
initial concentration: 0.0
is: CHEBI:16108
constant: false
name: 3phosphoglycerate cytosol

compartment 1
type: Compartment
is: GO:0005829
constant: true
size: 1.0
spatial dimensions: 3.0
name: cytosol

672101
type: SpeciesReference
stoichiometry: 1.0
species: species 23

species 23
type: Species
is: C00197
initial amount: NaN
is: http://identifiers.org/pubchem.substance/3497
name: 3phosphoglycerate
is: CHEBI:17794
constant: false
boundary condition: false
in compartment: compartment 2
has only substance units: false
initial concentration: 0.0

vPFK
type: Reaction
is homolog to: REACT 736
is: R00756
name: phophofructokinase
reversible: true
reactant: 671341
kinetic law: 671389
is version of: http://identifiers.org/ec-code/2.7.1.11
product: 671377
is version of: GO:0003872
reactant: 671353
fast: false
product: 671365

671341
type: SpeciesReference
stoichiometry: 1.0
species: species 15

species 15
type: Species
constant: false
is: http://identifiers.org/pubchem.substance/7723
is: CHEBI:16084
in compartment: compartment 2
initial amount: NaN
has only substance units: false
is: C05345
name: fructose6phosphate
initial concentration: 0.5
boundary condition: false

047972
type: LocalParameter
value: 10.7

047970
type: LocalParameter
value: 0.026

047968
type: LocalParameter
value: 0.82

047966
type: LocalParameter
value: 15.8

047964
type: LocalParameter
value: 1708.0

671377
type: SpeciesReference
stoichiometry: 1.0
species: species 12

species 12
type: Species
constant: false
has only substance units: false
is: CHEBI:16761
name: adpg
is: C00008
is: http://identifiers.org/pubchem.substance/3310
boundary condition: false
is: http://identifiers.org/cas/20398-34-9
initial amount: NaN
initial concentration: 1.51899628498
in compartment: compartment 2

671353
type: SpeciesReference
stoichiometry: 1.0
species: species 11

species 11
type: Species
initial amount: NaN
is: C00002
initial concentration: 0.240501857508
constant: false
boundary condition: false
is: http://identifiers.org/cas/56-65-5
has only substance units: false
name: atpg
in compartment: compartment 2
is: CHEBI:15422
is: http://identifiers.org/pubchem.substance/3304

671365
type: SpeciesReference
stoichiometry: 1.0
species: species 16

species 16
type: Species
initial amount: NaN
boundary condition: false
is: C05378
is: CHEBI:28013
has only substance units: false
in compartment: compartment 2
initial concentration: 10.0
constant: false
is: http://identifiers.org/pubchem.substance/7752
name: fructose16bisphosphate

metaid_0000071
type: Parameter
constant: true
value: 1.0
name: RaPFK

species 8
type: Species
boundary condition: false
initial concentration: 2.23134594788
is: CHEBI:16108
initial amount: NaN
is: C00111
is: http://identifiers.org/pubchem.substance/3411
has only substance units: false
name: dihydroxyacetonephosphate cytosol
constant: false
in compartment: compartment 1
is: B00029

vPT
type: Reaction
kinetic law: 671701
product: 671689
reactant: 671677
is version of: GO:0006849
is version of: GO:0050833
fast: false
reversible: false
name: pyruvate transport

048022
type: LocalParameter
value: 200.0

048020
type: LocalParameter
value: 1.96

671689
type: SpeciesReference
stoichiometry: 1.0
species: species 26

species 26
type: Species
is version of: CHEBI:32816
initial amount: NaN
is version of: C00022
is version of: http://identifiers.org/pubchem.substance/3324
name: pyruvate external
is version of: http://identifiers.org/cas/127-17-3
constant: true
boundary condition: true
initial concentration: 0.0
in compartment: compartment 3
has only substance units: false
is version of: B00006

671677
type: SpeciesReference
stoichiometry: 1.0
species: species 1

species 1
type: Species
boundary condition: false
initial concentration: 10.0
in compartment: compartment 1
is version of: B00006
initial amount: NaN
is version of: CHEBI:32816
name: pyruvate
is version of: http://identifiers.org/cas/127-17-3
is version of: C00022
constant: false
has only substance units: false
is version of: http://identifiers.org/pubchem.substance/3324

671173
type: SBMLUnit
exponent: 1.0
scale: 0
multipler: 1.0
kind: SECOND

species 21
type: Species
has only substance units: false
initial concentration: 0.5
constant: false
name: bisphosphoglycerate
is: CHEBI:16001
is: C00236
is: http://identifiers.org/cas/38168-82-0
initial amount: NaN
is: http://identifiers.org/pubchem.substance/3535
boundary condition: false
in compartment: compartment 2

species 22
type: Species
boundary condition: false
is: C00093
name: glycerol3phosphate
is: CHEBI:15978
is: http://identifiers.org/cas/57-03-4
in compartment: compartment 2
initial amount: NaN
initial concentration: 10.5208853981
is: http://identifiers.org/pubchem.substance/3393
constant: false
has only substance units: false

vGPDH
type: Reaction
is version of: http://identifiers.org/ec-code/3.1.3.21
kinetic law: 671629
is: R00841
is homolog to: REACT 1146
reactant: 671593
fast: false
reversible: true
product: 671605
name: glycerol3phosphatedehydrogenase
reactant: 671581
product: 671617
is version of: GO:0004367

048014
type: LocalParameter
value: 2.0

048012
type: LocalParameter
value: 0.4

048010
type: LocalParameter
value: 0.28

048008
type: LocalParameter
value: 0.01

048006
type: LocalParameter
value: 0.1

048004
type: LocalParameter
value: 465.0

671593
type: SpeciesReference
stoichiometry: 1.0
species: species 20

species 20
type: Species
has only substance units: false
initial concentration: 2.0
is: http://identifiers.org/pubchem.substance/3306
constant: false
is: C00004
boundary condition: false
in compartment: compartment 2
initial amount: NaN
name: nadh
is: CHEBI:16908

671605
type: SpeciesReference
stoichiometry: 1.0
species: species 19

species 19
type: Species
initial amount: NaN
is: http://identifiers.org/cas/53-84-9
boundary condition: false
in compartment: compartment 2
constant: false
is: http://identifiers.org/pubchem.substance/3305
name: nad
has only substance units: false
is: C00003
is: CHEBI:15846
initial concentration: 2.0

671581
type: SpeciesReference
stoichiometry: 1.0
species: species 17

species 17
type: Species
is: CHEBI:16108
initial amount: NaN
in compartment: compartment 2
name: dihydroxyacetonephosphate
initial concentration: 8.47911460193
is: C00111
is: http://identifiers.org/pubchem.substance/3411
has only substance units: false
is: B00029
constant: false
boundary condition: false

671617
type: SpeciesReference
stoichiometry: 1.0
species: species 22

vPGI
type: Reaction
is homolog to: REACT 1164
is version of: http://identifiers.org/ec-code/5.3.1.9
reactant: 671305
fast: false
reversible: true
kinetic law: 671329
is: R00771
is version of: GO:0004347
name: phosphoglycerate isomerase
product: 671317

671305
type: SpeciesReference
stoichiometry: 1.0
species: species 14

species 14
type: Species
is: CHEBI:4170
name: glucose6phosphate
is: C00668
has only substance units: false
initial amount: NaN
boundary condition: false
initial concentration: 0.5
is: http://identifiers.org/pubchem.substance/3937
in compartment: compartment 2
constant: false

047962
type: LocalParameter
value: 1305.0

047960
type: LocalParameter
value: 1305.0

047958
type: LocalParameter
value: 0.12

047956
type: LocalParameter
value: 0.4

671317
type: SpeciesReference
stoichiometry: 1.0
species: species 15

species 4
type: Species
name: phosphoenolpyruvate
is: http://identifiers.org/pubchem.substance/3374
in compartment: compartment 1
initial amount: NaN
is: C00074
constant: false
is: B00019
boundary condition: false
has only substance units: false
is: http://identifiers.org/cas/138-08-9
initial concentration: 0.0

species 3
type: Species
name: atpc
initial amount: NaN
constant: false
is: C00002
in compartment: compartment 1
has only substance units: false
is: http://identifiers.org/pubchem.substance/3304
is: http://identifiers.org/cas/56-65-5
boundary condition: false
initial concentration: 0.341738611875
is: CHEBI:15422

vAU
type: Reaction
kinetic law: 671860
product: 671848
reactant: 671836
is version of: GO:0006200
fast: false
reversible: false
name: atp utilisation

048042
type: LocalParameter
value: 50.0

671848
type: SpeciesReference
stoichiometry: 1.0
species: species 2

species 2
type: Species
in compartment: compartment 1
is: CHEBI:16761
is: http://identifiers.org/pubchem.substance/3310
initial amount: NaN
is: http://identifiers.org/cas/20398-34-9
constant: false
boundary condition: false
is: C00008
has only substance units: false
name: adpc
initial concentration: 1.31652277625

671836
type: SpeciesReference
stoichiometry: 1.0
species: species 3

metaid_0000072
type: Parameter
constant: true
value: 1.0
name: RaPYK

vGlyT
type: Reaction
kinetic law: 672221
product: 672209
reactant: 672197
is version of: GO:0015793
fast: false
reversible: true
name: glycerol transport

048090
type: LocalParameter
value: 1000000.0

048088
type: LocalParameter
value: 1000000.0

672209
type: SpeciesReference
stoichiometry: 1.0
species: species 27

species 27
type: Species
in compartment: compartment 3
is: B00032
name: glycerol external
boundary condition: true
is: CHEBI:17754
initial amount: NaN
constant: true
is: C00116
has only substance units: false
is: http://identifiers.org/pubchem.substance/3416
is: http://identifiers.org/cas/56-81-5
initial concentration: 0.0

672197
type: SpeciesReference
stoichiometry: 1.0
species: species 24

species 24
type: Species
is: CHEBI:17754
name: glycerol
initial amount: NaN
is: http://identifiers.org/cas/56-81-5
boundary condition: false
in compartment: compartment 2
initial concentration: 0.0
is: C00116
constant: false
is: B00032
is: http://identifiers.org/pubchem.substance/3416
has only substance units: false

vPK
type: Reaction
fast: false
is homolog to: REACT 1911
product: 671799
reactant: 671774
product: 671811
is version of: http://identifiers.org/ec-code/2.7.1.40
name: pyruvate kinase
reactant: 671787
is version of: GO:0004743
reversible: true
kinetic law: 671823
is: R00200

671799
type: SpeciesReference
stoichiometry: 1.0
species: species 1

671774
type: SpeciesReference
stoichiometry: 1.0
species: species 4

671811
type: SpeciesReference
stoichiometry: 1.0
species: species 3

671787
type: SpeciesReference
stoichiometry: 1.0
species: species 2

048040
type: LocalParameter
value: 0.114

048038
type: LocalParameter
value: 2.5

048036
type: LocalParameter
value: 1020.0

metaid_0000073
type: Parameter
constant: true
value: 1.0
name: RaPGAM

vAKg
type: Reaction
reactant: 672053
is version of: GO:0004017
is: R00127
reactant: 672065
kinetic law: 672089
name: adenylate kinase glycosome
is version of: http://identifiers.org/ec-code/2.7.4.3
reversible: true
product: 672077
fast: false
is homolog to: REACT 643

672053
type: SpeciesReference
stoichiometry: 1.0
species: species 11

672065
type: SpeciesReference
stoichiometry: 1.0
species: species 13

species 13
type: Species
name: ampg
constant: false
initial concentration: 4.24050185751
is: C00020
boundary condition: false
is: CHEBI:16027
in compartment: compartment 2
has only substance units: false
is: http://identifiers.org/pubchem.substance/3322
initial amount: NaN
is: http://identifiers.org/cas/61-19-8

048078
type: LocalParameter
value: 0.442

048076
type: LocalParameter
value: 1000000.0

672077
type: SpeciesReference
stoichiometry: 2.0
species: species 12

species 18
type: Species
boundary condition: false
is: http://identifiers.org/cas/591-57-1
has only substance units: false
initial concentration: 2.5
name: glyceraldehyde3phosphate
is: http://identifiers.org/pubchem.substance/3418
in compartment: compartment 2
constant: false
initial amount: NaN
is: CHEBI:29052
is: C00118

species 6
type: Species
boundary condition: false
constant: false
is: CHEBI:16027
is: http://identifiers.org/cas/61-19-8
is: C00020
initial concentration: 2.24173861188
initial amount: NaN
has only substance units: false
in compartment: compartment 1
is: http://identifiers.org/pubchem.substance/3322
name: ampc

vANTI
type: Reaction
kinetic law: 672185
reactant: 672149
reversible: true
fast: false
is version of: GO:0015169
product: 672173
name: gly3p dhap antiporter
reactant: 672137
product: 672161

048086
type: LocalParameter
value: 1000000.0

048084
type: LocalParameter
value: 1000000.0

672149
type: SpeciesReference
stoichiometry: 1.0
species: species 8

672173
type: SpeciesReference
stoichiometry: 1.0
species: species 17

672137
type: SpeciesReference
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species: species 22

672161
type: SpeciesReference
stoichiometry: 1.0
species: species 9

species 9
type: Species
initial amount: NaN
is: http://identifiers.org/cas/57-03-4
is: C00093
in compartment: compartment 1
is: http://identifiers.org/pubchem.substance/3393
initial concentration: 2.76865405212
boundary condition: false
name: glycerol3phosphate cytosol
is: CHEBI:15978
has only substance units: false
constant: false

vPGM
type: Reaction
reactant: 671933
is: R01518
is version of: GO:0004619
is homolog to: REACT 576
reversible: true
kinetic law: 671957
product: 671945
is version of: http://identifiers.org/ec-code/5.4.2.1
fast: false
name: phosphoglycerate mutase

671933
type: SpeciesReference
stoichiometry: 1.0
species: species 7

048062
type: LocalParameter
value: 495.0

048060
type: LocalParameter
value: 225.0

048058
type: LocalParameter
value: 0.11

048056
type: LocalParameter
value: 0.27

671945
type: SpeciesReference
stoichiometry: 1.0
species: species 5

species 5
type: Species
is: CHEBI:17835
initial concentration: 0.0
in compartment: compartment 1
has only substance units: false
name: 2phosphoglycerate
initial amount: NaN
boundary condition: false
is: http://identifiers.org/pubchem.substance/3904
constant: false
is: C00631

vTPI
type: Reaction
kinetic law: 671509
is version of: http://identifiers.org/ec-code/5.3.1.1
is version of: GO:0004807
reversible: true
fast: false
product: 671497
name: triosephosphate isomerase
reactant: 671485
is homolog to: REACT 775
is: R01015

047990
type: LocalParameter
value: 5696.01

047988
type: LocalParameter
value: 999.3

047986
type: LocalParameter
value: 0.25

047984
type: LocalParameter
value: 1.2

671497
type: SpeciesReference
stoichiometry: 1.0
species: species 18

671485
type: SpeciesReference
stoichiometry: 1.0
species: species 17

vENO
type: Reaction
is version of: GO:0004634
product: 671981
reversible: true
kinetic law: 671993
name: enolase
is version of: http://identifiers.org/ec-code/4.2.1.11
fast: false
is: R00658
reactant: 671969
is homolog to: REACT 1400

671981
type: SpeciesReference
stoichiometry: 1.0
species: species 4

048070
type: LocalParameter
value: 394.68

048068
type: LocalParameter
value: 598.0

048066
type: LocalParameter
value: 0.24

048064
type: LocalParameter
value: 0.054

671969
type: SpeciesReference
stoichiometry: 1.0
species: species 5

vGPO
type: Reaction
fast: false
is version of: GO:0004369
reversible: false
is: R00846
product: 671653
kinetic law: 671665
is version of: http://identifiers.org/ec-code/1.1.3.21
reactant: 671641
name: glycerol3phosphate oxidase

671653
type: SpeciesReference
stoichiometry: 1.0
species: species 8

048018
type: LocalParameter
value: 368.0

048016
type: LocalParameter
value: 1.7

671641
type: SpeciesReference
stoichiometry: 1.0
species: species 9

671185
type: SBMLUnit
exponent: 1.0
scale: -3
multipler: 1.0
kind: MOLE

vGK
type: Reaction
product: 671909
product: 671897
name: glycerol kinase
is: R00847
is homolog to: REACT 724
reactant: 671885
is version of: http://identifiers.org/ec-code/2.7.1.30
kinetic law: 671921
is version of: GO:0004370
fast: false
reactant: 671872
reversible: true

671909
type: SpeciesReference
stoichiometry: 1.0
species: species 11

671897
type: SpeciesReference
stoichiometry: 1.0
species: species 24

671885
type: SpeciesReference
stoichiometry: 1.0
species: species 12

048054
type: LocalParameter
value: 0.24

048052
type: LocalParameter
value: 0.44

048050
type: LocalParameter
value: 60.86

048048
type: LocalParameter
value: 0.56

048046
type: LocalParameter
value: 3.83

048044
type: LocalParameter
value: 200.0

671872
type: SpeciesReference
stoichiometry: 1.0
species: species 22

vHK
type: Reaction
reactant: 671257
fast: false
product: 671269
is version of: http://identifiers.org/ec-code/2.7.1.2
is: R00299
reactant: 671245
is version of: GO:0004396
reversible: true
product: 671281
kinetic law: 671293
name: hexokinase
is homolog to: REACT 1318

671257
type: SpeciesReference
stoichiometry: 1.0
species: species 11

671269
type: SpeciesReference
stoichiometry: 1.0
species: species 14

671245
type: SpeciesReference
stoichiometry: 1.0
species: species 10

671281
type: SpeciesReference
stoichiometry: 1.0
species: species 12

047954
type: LocalParameter
value: 12.0

047952
type: LocalParameter
value: 0.126

047950
type: LocalParameter
value: 0.116

047948
type: LocalParameter
value: 0.1

047946
type: LocalParameter
value: 1929.0

metaid_0000074
type: Parameter
constant: true
value: 1.0
name: RaENO

671197
type: SBMLUnit
exponent: 1.0
scale: -3
multipler: 1.0
kind: LITRE

vPGK
type: Reaction
is homolog to: REACT 1186
kinetic law: 671762
name: phosphoglycerate kinase
fast: false
product: 671750
product: 671738
is version of: http://identifiers.org/ec-code/2.7.2.3
reversible: true
reactant: 671726
reactant: 671713
is version of: GO:0004618
is: R01512

048034
type: LocalParameter
value: 0.29

048032
type: LocalParameter
value: 1.62

048030
type: LocalParameter
value: 0.47

048028
type: LocalParameter
value: 0.1

048026
type: LocalParameter
value: 0.003

048024
type: LocalParameter
value: 2862.0

671750
type: SpeciesReference
stoichiometry: 1.0
species: species 11

671738
type: SpeciesReference
stoichiometry: 1.0
species: species 23

671726
type: SpeciesReference
stoichiometry: 1.0
species: species 12

671713
type: SpeciesReference
stoichiometry: 1.0
species: species 21

ORG:
  orgname: Humboldt University Berlin
n:
  given: Marvin
  family: Schulz

vAKc
type: Reaction
fast: false
kinetic law: 672041
name: adenylate kinase cytosol
reversible: true
is version of: http://identifiers.org/ec-code/2.7.4.3
product: 672029
reactant: 672017
reactant: 672005
is homolog to: REACT 643
is: R00127
is version of: GO:0004017

048074
type: LocalParameter
value: 0.442

048072
type: LocalParameter
value: 1000000.0

672029
type: SpeciesReference
stoichiometry: 2.0
species: species 2

672017
type: SpeciesReference
stoichiometry: 1.0
species: species 6

672005
type: SpeciesReference
stoichiometry: 1.0
species: species 3

vALD
type: Reaction
modifier: 671437
fast: false
is version of: GO:0004332
name: aldolase
is homolog to: REACT 1602
product: 671413
reactant: 671401
reversible: true
is: R01070
is version of: http://identifiers.org/ec-code/4.1.2.13
product: 671425
kinetic law: 671473
modifier: 671449
modifier: 671461

671413
type: SpeciesReference
stoichiometry: 1.0
species: species 17

671401
type: SpeciesReference
stoichiometry: 1.0
species: species 16

671425
type: SpeciesReference
stoichiometry: 1.0
species: species 18

047982
type: LocalParameter
value: 0.098

047980
type: LocalParameter
value: 0.067

047978
type: LocalParameter
value: 1.19

047976
type: LocalParameter
value: 0.069

047974
type: LocalParameter
value: 560.0

vGAPDH
type: Reaction
kinetic law: 671569
product: 671545
reactant: 671533
fast: false
reactant: 671521
product: 671557
is: R01061
is version of: GO:0043878
name: glyceraldehyde3phosphatedehydrogenase
is version of: http://identifiers.org/ec-code/1.2.1.12
reversible: true
is homolog to: REACT 1847

048002
type: LocalParameter
value: 0.02

048000
type: LocalParameter
value: 0.1

047998
type: LocalParameter
value: 0.67

047996
type: LocalParameter
value: 0.45

047994
type: LocalParameter
value: 0.15

047992
type: LocalParameter
value: 720.9

671545
type: SpeciesReference
stoichiometry: 1.0
species: species 21

671533
type: SpeciesReference
stoichiometry: 1.0
species: species 19

671521
type: SpeciesReference
stoichiometry: 1.0
species: species 18

671557
type: SpeciesReference
stoichiometry: 1.0
species: species 20

W3 CDTF: Tue Jan 27 14:07:41 GMT 2009

ORG:
  orgname: EMBL-EBI
n:
  given: Vijayalakshmi
  family: Chelliah

ORG:
  orgname: Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology, Belgium.
n:
  given: Paul A. M.
  family: Michels

W3 CDTF: Tue May 15 22:59:43 BST 2012