BIOMD0000000186
type: SBMLModel
created:
  W3 CDTF: Tue Sep 02 16:06:57 BST 2008
species: metaid_0000324
time units: metaid_0000054
event: metaid_0000031
reaction: metaid_0000023
curated: true
length unit: length
species: metaid_0000021
is described by: http://identifiers.org/pubmed/18253502
creator:
  ORG:
    orgname: German Cancer Research Center, Heidelberg
  n:
    given: Bashar
    family: Ibrahim
parameter: metaid_0000012
parameter: metaid_0000043
species: metaid_0000015
species: metaid_0000016
species: metaid_0000019
parameter: metaid_0000006
creator:
  ORG:
    orgname: Fritz Lipmann Institute, Jena, Germany
  n:
    given: Eberhard
    family: Schmitt
is: MODEL6655615431
reaction: metaid_0000223
parameter: metaid_0000011
reaction: metaid_0000027
is version of: GO:0007094
reaction: metaid_0000026
occurs in: http://identifiers.org/taxonomy/9606
parameter: metaid_0000612
species: metaid_0000322
creator:
  ORG:
    orgname: Friedrich-Schiller-University Jena, Germany
  n:
    given: Peter
    family: Dittrich
volume units: metaid_0000004
parameter: metaid_0000009
notes: Ibrahim2008 - Mitotic Spindle Assembly Checkpoint - Dissociation variant The Mitotic Spindle Assembly Checkpoint ((M)SAC) is an evolutionary conserved mechanism. This model incorporates the perspectives of three central control pathways, namely Mad1/Mad2 induced Cdc20 sequestering based on the Template Model, MCC formation, and APC inhibition. MCC:APC dissociation is described by two alternatives models, namely the "Dissociation" and the "Convey" model variants. Both these model are available in BioModels Database. This model corresponds to the "Dissociation" variant. This model is described in the article: In-silico modeling of the mitotic spindle assembly checkpoint. Ibrahim B, Diekmann S, Schmitt E, Dittrich P PLoS One. 2008 Feb 6;3(2):e1555. Abstract: BACKGROUND: The Mitotic Spindle Assembly Checkpoint ((M)SAC) is an evolutionary conserved mechanism that ensures the correct segregation of chromosomes by restraining cell cycle progression from entering anaphase until all chromosomes have made proper bipolar attachments to the mitotic spindle. Its malfunction can lead to cancer. PRINCIPLE FINDINGS: We have constructed and validated for the human (M)SAC mechanism an in silico dynamical model, integrating 11 proteins and complexes. The model incorporates the perspectives of three central control pathways, namely Mad1/Mad2 induced Cdc20 sequestering based on the Template Model, MCC formation, and APC inhibition. Originating from the biochemical reactions for the underlying molecular processes, non-linear ordinary differential equations for the concentrations of 11 proteins and complexes of the (M)SAC are derived. Most of the kinetic constants are taken from literature, the remaining four unknown parameters are derived by an evolutionary optimization procedure for an objective function describing the dynamics of the APC:Cdc20 complex. MCC:APC dissociation is described by two alternatives, namely the "Dissociation" and the "Convey" model variants. The attachment of the…
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units def: metaid_0000041
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name: Ibrahim2008 - Mitotic Spindle Assembly Checkpoint - Dissociation variant
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parameter: metaid_0000004
species: metaid_0000022
substance units: metaid_0000053
modified:
  W3 CDTF: Tue Jan 08 15:43:49 GMT 2013
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is version of: REACT 2137.2
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creator:
  ORG:
    orgname: EMBL-EBI
  n:
    given: Lukas
    family: Endler
parameter: metaid_0000812
reaction: metaid_0000225
parameter: metaid_1000007
creator:
  ORG:
    orgname: Fritz Lipmann Institute, Jena, Germany
  n:
    given: Stephan
    family: Diekmann
reaction: metaid_0000224
parameter: metaid_0000512

metaid_0000324
type: Species
constant: false
has part: Q9UM13
name: APC:Cdc20
initial amount: NaN
substance units: mole
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has part: Q9NYG5
has part: Q9UJX3
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boundary condition: false
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initial concentration: 0.0

metaid_0000014
type: Compartment
is: GO:0005737
constant: true
size: 1.0
spatial dimensions: 3.0
name: Cytoplasm

metaid_0000031
type: Event
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event assignment: 725871
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use values from trigger time: true
name: Microtubule attachment

metaid_1000007
type: Parameter
notes: u = 0 : kinetochore unattached u = 1 : kinetochore attached
constant: false
units: dimensionless
value: 0.0

metaid_0000007
type: Parameter
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units: dimensionless
value: 1.0

725859
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persistant: true
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metaid_0000016
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notes: Open conformation of Mad2
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name: O-Mad2
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725415
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name: Mad1:C-Mad2
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substance units: mole
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constant: false
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boundary condition: false
name: Mad1:C-Mad2:O-Mad2*
initial amount: NaN

metaid_0000021
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name: MCC
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is: GO:0033597
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constant: true
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value: 0.02

metaid_0000040
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name: per second

725365
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exponent: -1.0
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type: Parameter
constant: true
units: dimensionless
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metaid_0000019
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boundary condition: false
substance units: mole
has part: Q13257
initial concentration: 0.0
name: Cdc20:C-Mad2
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constant: false
has part: Q12834
initial amount: NaN

metaid_0000006
type: Parameter
constant: true
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value: 0.2

metaid_0000223
type: Reaction
reactant: 725727
fast: false
is version of: GO:0043623
reversible: false
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is version of: GO:0051436
kinetic law: 725751

725727
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metaid_0000322
type: Species
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has part: Q9UJX3
initial concentration: 9.0E-8
has only substance units: false
is version of: GO:0005680
in compartment: metaid_0000014
has part: P30260
has part: Q13042
has part: Q9UJX2
name: APC
has part: Q8NHZ8
has part: Q9BS18
substance units: mole

725715
type: SpeciesReference
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725739
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metaid_0000323
type: Species
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name: MCC:APC
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has part: GO:0033597
substance units: mole
has part: Q9UJX2
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constant: false
has part: Q9NYG5

metaid_0000011
type: Parameter
constant: true
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value: 1.0E7

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unit: 725379
name: liter per mole per second

725403
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kind: SECOND

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kind: LITRE

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reversible: true
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name: Cdc20
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value: 1.0E8

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constant: true
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value: 5000000.0

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constant: true
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value: 1.0E8