931154
kinetic law: 202247
product: 202235
reactant: 202223
reactant: 202211
notes: Hasler et al FASEB J 6:2734-2741 1992 say Kd =1e-7M for type II, 5.6e-8 M for type I. Take mean which comes to 2e-13 #/cell Smith et al PNAS USA 78:3 1591-1595 1981 have better data. First kf/kb=2.1e7/M = 3.5e-5 (#/cell). Ogreid and Doskeland Febs Lett 129:2 287-292 1981 have figs suggesting time course of complete assoc is < 1 min.
fast: false
reversible: true
type: Reaction

203278
local parameters: 298042
local parameters: 298040
notes: kcat*AC_slash_Gs_dot_AC*AC_slash_ATP/(Km+AC_slash_ATP)
type: KineticLaw

202699
stoichiometry: 1.0
species: 931054
type: SpeciesReference

931574
kinetic law: 203229
product: 203217
reactant: 203205
fast: false
reversible: false
type: Reaction

202272
stoichiometry: 1.0
species: 930754
type: SpeciesReference

297983
units: 202053
value: 3.3E-11
type: LocalParameter

298036
units: 202139
value: 5.04E-12
type: LocalParameter

297993
units: 202053
value: 1.0E-12
type: LocalParameter

298026
units: 202053
value: 1.0E-13
type: LocalParameter

298006
units: 202090
value: 0.0
type: LocalParameter

930634
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

931554
kinetic law: 203193
product: 203181
reactant: 203169
reactant: 203157
notes: This form has about twice the activity of the unphosphorylated form. See Sette et al JBC 269:28 18271-18274 1994. We\'ll ignore cGMP effects for now.
fast: false
reversible: true
type: Reaction

298016
units: 202139
value: 4.99998E-12
type: LocalParameter

202832
stoichiometry: 1.0
species: 931134
type: SpeciesReference

298035
units: 202053
value: 1.0E-11
type: LocalParameter

297982
units: 202139
value: 5.4E-11
type: LocalParameter

297992
units: 202139
value: 6.0E-11
type: LocalParameter

931534
kinetic law: 203145
product: 203133
reactant: 203121
fast: false
reversible: false
type: Reaction

931514
kinetic law: 203109
product: 203097
reactant: 203085
reactant: 203073
notes: Best rates are from Conti et al Biochem 34 7979-7987 1995. Though these are for the Sertoli cell form, it looks like they carry nicely into alternatively spliced brain form. See Sette et al JBC 269:28 18271-18274 Km ~2 uM, Vmax est ~ 10 umol/min/mg for pure form. Brain protein is 93 kD but this was 67. So k3 ~10, k2 ~40, k1 ~4.2e-6
fast: false
reversible: true
type: Reaction

202369
stoichiometry: 1.0
species: 930674
type: SpeciesReference

203229
local parameters: 298038
notes: k3*AC_slash_cAMP_minus_PDE_star__slash_PDE_star__slash_PDE_star__cplx
type: KineticLaw

202976
stoichiometry: 1.0
species: 930814
type: SpeciesReference

202771
stoichiometry: 1.0
species: 930994
type: SpeciesReference

297981
units: 202053
value: 3.3E-11
type: LocalParameter

298038
units: 202053
value: 2.0E-11
type: LocalParameter

298024
units: 202139
value: 1.00002E-11
type: LocalParameter

202808
stoichiometry: 1.0
species: 931034
type: SpeciesReference

202284
stoichiometry: 1.0
species: 930654
type: SpeciesReference

202687
stoichiometry: 1.0
species: 931014
type: SpeciesReference

297995
units: 202053
value: 0.0
type: LocalParameter

202783
local parameters: 298010
local parameters: 298008
notes: kf*Gs_slash_GDP_dot_Ga*Gs_slash_Gbg-kb*Gs_slash_GDP_dot_Gabc
type: KineticLaw

202199
exponent: 1.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

931474
kinetic law: 203012
product: 203000
reactant: 202988
reactant: 202976
notes: See Bramson et al CRC crit rev Biochem 15:2 93-124. The rates there are for peptide substrates and too fast. Scaled down by a factor of 3 as per Cohen et al FEBS Lett 76:182-86 (1977).
fast: false
reversible: true
type: Reaction

298018
units: 202053
value: 1.0E-13
type: LocalParameter

298004
units: 202053
value: 2.5E-14
type: LocalParameter

202441
local parameters: 297989
local parameters: 297988
notes: kf*PKA_slash_cAMP4_dot_R2C2-kb*PKA_minus_active*PKA_slash_cAMP4_dot_R2C
type: KineticLaw

930674
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

298037
units: 202053
value: 8.0E-11
type: LocalParameter

202102
exponent: 3.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

930536
constant: true
size: 1.0E-18
spatial dimensions: 3.0
type: Compartment

202247
local parameters: 297981
local parameters: 297980
notes: kf*PKA_slash_R2C2*cAMP-kb*PKA_slash_cAMP_dot_R2C2
type: KineticLaw

297980
units: 202139
value: 5.4E-11
type: LocalParameter

202453
stoichiometry: 1.0
species: 930714
type: SpeciesReference

203000
stoichiometry: 1.0
species: 930794
type: SpeciesReference

202820
local parameters: 298014
local parameters: 298012
notes: kf*GTP_dot_Ga-kb*Gs_slash_GDP_dot_Ga
type: KineticLaw

297994
units: 202053
value: 1.0E-13
type: LocalParameter

202649
stoichiometry: 1.0
species: 931134
type: SpeciesReference

202308
stoichiometry: 1.0
species: 930654
type: SpeciesReference

202988
stoichiometry: 1.0
species: 930774
type: SpeciesReference

930814
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.5
initial amount: NaN
in compartment: 930554
type: Species

203061
local parameters: 298032
notes: k3*PKA_minus_active_slash_phosph_minus_PDE_slash_phosph_minus_PDE_cplx
type: KineticLaw

203169
stoichiometry: 1.0
species: 930854
type: SpeciesReference

931114
notes: Assumed buffered to 1 mM. Value is irrelevant to simulation.
constant: true
boundary condition: true
has only substance units: false
initial concentration: 1000.0
initial amount: NaN
in compartment: 930554
type: Species

203109
local parameters: 298034
local parameters: 298033
notes: k1*cAMP*AC_slash_cAMP_minus_PDE-k2*AC_slash_cAMP_minus_PDE_slash_PDE_slash_PDE_cplx
type: KineticLaw

930774
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

202357
stoichiometry: 1.0
species: 930754
type: SpeciesReference

297987
units: 202053
value: 3.25E-11
type: LocalParameter

202163
exponent: -1.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

202904
stoichiometry: 1.0
species: 931074
type: SpeciesReference

298012
units: 202053
value: 6.6667E-14
type: LocalParameter

202880
stoichiometry: 1.0
species: 930994
type: SpeciesReference

931234
kinetic law: 202441
product: 202429
product: 202417
reactant: 202405
notes: This has to be fast, as the activation of PKA by cAMP is also fast. kf was 10
fast: false
reversible: true
type: Reaction

202795
stoichiometry: 1.0
species: 930934
type: SpeciesReference

202417
stoichiometry: 1.0
species: 930774
type: SpeciesReference

202868
local parameters: 298018
local parameters: 298016
notes: kf*Gs_slash_L*Gs_slash_R_dot_GDP_dot_Gabc-kb*Gs_slash_L_dot_R_dot_GDP_dot_Gabc
type: KineticLaw

298002
units: 202053
value: 1.0E-13
type: LocalParameter

297986
units: 202139
value: 7.5E-11
type: LocalParameter

931274
kinetic law: 202539
product: 202526
reactant: 202514
reactant: 202502
notes: This has to be set to zero for matching the expts in vitro. In vivo we need to consider the inhibition though.
fast: false
reversible: true
type: Reaction

202345
local parameters: 297985
local parameters: 297984
notes: kf*PKA_slash_cAMP2_dot_R2C2*cAMP-kb*PKA_slash_cAMP3_dot_R2C2
type: KineticLaw

931294
kinetic law: 202575
product: 202563
reactant: 202551
notes: The rates for this are poorly constrained. In adipocytes (probably a different PDE) the dephosphorylation is complete within 15 min, but there are no intermediate time points so it could be much faster. Identity of phosphatase etc is still unknown.
fast: false
reversible: true
type: Reaction

202952
stoichiometry: 1.0
species: 931014
type: SpeciesReference

203073
stoichiometry: 1.0
species: 930754
type: SpeciesReference

203157
stoichiometry: 1.0
species: 930754
type: SpeciesReference

297996
units: 202139
value: 4.99998E-10
type: LocalParameter

202856
stoichiometry: 1.0
species: 931014
type: SpeciesReference

930854
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

931254
kinetic law: 202489
product: 202477
product: 202465
reactant: 202453
fast: false
reversible: true
type: Reaction

202502
stoichiometry: 1.0
species: 930774
type: SpeciesReference

202175
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

930994
constant: false
boundary condition: false
has only substance units: false
initial concentration: 1.0
initial amount: NaN
in compartment: 930554
type: Species

297985
units: 202053
value: 1.1E-10
type: LocalParameter

298028
units: 202139
value: 6.0E-12
type: LocalParameter

203097
stoichiometry: 1.0
species: 930834
type: SpeciesReference

930614
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

298014
units: 202053
value: 0.0
type: LocalParameter

203145
local parameters: 298035
notes: k3*AC_slash_cAMP_minus_PDE_slash_PDE_slash_PDE_cplx
type: KineticLaw

298000
units: 202139
value: 1.00002E-13
type: LocalParameter

202892
stoichiometry: 1.0
species: 930954
type: SpeciesReference

202260
stoichiometry: 1.0
species: 930634
type: SpeciesReference

202405
stoichiometry: 1.0
species: 930694
type: SpeciesReference

202964
local parameters: 298026
local parameters: 298024
notes: kf*Gs_slash_GDP_dot_Gabc*Gs_slash_L_dot_R-kb*Gs_slash_L_dot_R_dot_GDP_dot_Gabc
type: KineticLaw

202078
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

930714
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

930914
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.015
initial amount: NaN
in compartment: 930554
type: Species

203085
stoichiometry: 1.0
species: 930814
type: SpeciesReference

297984
units: 202139
value: 7.5E-11
type: LocalParameter

297998
units: 202053
value: 1.0E-12
type: LocalParameter

931594
kinetic law: 203278
modifier: 203266
product: 203254
reactant: 203241
notes: See Feinstein et al PNAS 88:10173-10177, Jacobowitz et al JBC 286(6):3829-3832 Smigel, JBC 261(4):1976-1982 (1986)
fast: false
reversible: false
type: Reaction

202333
stoichiometry: 1.0
species: 930674
type: SpeciesReference

930954
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0833333333333333
initial amount: NaN
in compartment: 930554
type: Species

931214
kinetic law: 202393
product: 202381
reactant: 202369
reactant: 202357
fast: false
reversible: true
type: Reaction

202844
stoichiometry: 1.0
species: 931074
type: SpeciesReference

202489
local parameters: 297991
local parameters: 297990
notes: kf*PKA_slash_cAMP4_dot_R2C-kb*PKA_minus_active*PKA_slash_cAMP4_dot_R2
type: KineticLaw

MODEL9079740062
reaction: 931514
volume units: 679943
units def: 202139
species: 930734
reaction: 931334
reaction: 931234
reaction: 931374
reaction: 931474
reaction: 931274
species: 930774
reaction: 931574
species: 930594
time units: 131570
name: Bhalla2004_PKA_2003
reaction: 931534
species: 930914
reaction: 931354
reaction: 931154
reaction: 931394
species: 930674
units def: 202090
notes: This model consists of receptor-ligand interaction, G-protein activation, Adenylyl cyclase mediated formation of cAMP and activation of PKA in the neuron. Demonstration programs using this model described in Bhalla US. (2004) Biophys J. 87(2):733-44 to generate a dose-response curve using stochastic calculations are available here. This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2011 The BioModels.net Team. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not.. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.
reaction: 931314
species: 930794
units def: 202016
length unit: length
species: 931094
substance units: 202187
compartment: 930554
species: 931114
species: 930854
reaction: 931254
species: 931014
is described by: http://identifiers.org/pubmed/15298882
species: 930634
species: 931054
species: 930974
reaction: 931194
reaction: 931434
units def: 202053
species: 931074
species: 930694
type: SBMLModel
reaction: 931214
species: 930754
species: 930574
reaction: 931294
species: 930934
species: 930834
species: 931134
compartment: 930536
species: 930874
species: 930814
units def: 202187
species: 930894
reaction: 931174
reaction: 931414
species: 931034
species: 930654
species: 930954
species: 930714
species: 930994
area units: area
reaction: 931594
reaction: 931554
species: 930614
reaction: 931494
reaction: 931454
curated: false

202526
stoichiometry: 1.0
species: 930614
type: SpeciesReference

202223
stoichiometry: 1.0
species: 930754
type: SpeciesReference

202759
stoichiometry: 1.0
species: 931054
type: SpeciesReference

202321
stoichiometry: 1.0
species: 930754
type: SpeciesReference

203193
local parameters: 298037
local parameters: 298036
notes: k1*cAMP*AC_slash_cAMP_minus_PDE_star_-k2*AC_slash_cAMP_minus_PDE_star__slash_PDE_star__slash_PDE_star__cplx
type: KineticLaw

930654
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

202429
stoichiometry: 1.0
species: 930714
type: SpeciesReference

202588
stoichiometry: 1.0
species: 930914
type: SpeciesReference

203121
stoichiometry: 1.0
species: 930834
type: SpeciesReference

202916
local parameters: 298022
local parameters: 298020
notes: kf*Gs_slash_GDP_dot_Gabc*Gs_slash_R-kb*Gs_slash_R_dot_GDP_dot_Gabc
type: KineticLaw

203048
stoichiometry: 1.0
species: 930774
type: SpeciesReference

202612
stoichiometry: 1.0
species: 930894
type: SpeciesReference

203241
stoichiometry: 1.0
species: 931094
type: SpeciesReference

202514
stoichiometry: 1.0
species: 930594
type: SpeciesReference

931434
kinetic law: 202916
product: 202904
reactant: 202892
reactant: 202880
notes: Receptor binding to Gs. Scale it to the same slow rates described by Fay et al for L.R to L.R.G. From detailed balance, Kd is 50.
fast: false
reversible: true
type: Reaction

931414
kinetic law: 202868
product: 202856
reactant: 202844
reactant: 202832
notes: From Seifert et al Mol. Pharmacol 56:348-358 (1999) The EC50 for ISO is about 20 nM.
fast: false
reversible: true
type: Reaction

202575
local parameters: 297995
local parameters: 297994
notes: kf*AC_slash_cAMP_minus_PDE_star_-kb*AC_slash_cAMP_minus_PDE
type: KineticLaw

202028
exponent: -1.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

202940
stoichiometry: 1.0
species: 930974
type: SpeciesReference

202235
stoichiometry: 1.0
species: 930634
type: SpeciesReference

297989
units: 202139
value: 1.8E-11
type: LocalParameter

202066
exponent: 1.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

203181
stoichiometry: 1.0
species: 930874
type: SpeciesReference

202114
exponent: -2.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

931494
kinetic law: 203061
product: 203048
product: 203036
reactant: 203024
fast: false
reversible: false
type: Reaction

931314
kinetic law: 202624
product: 202612
reactant: 202600
reactant: 202588
notes: Half-max at around 3nM = kb/kf from fig 5 in Feinstein et al PNAS USA 88 10173-10177 1991 kf = kb/1800 = 5.56e-4 kb Ofer Jacobowitz thesis (Mount Sinai 1995) data indicates it is more like 2 nM.
fast: false
reversible: true
type: Reaction

203133
stoichiometry: 1.0
species: 930814
type: SpeciesReference

202928
stoichiometry: 1.0
species: 930994
type: SpeciesReference

298010
units: 202053
value: 0.0
type: LocalParameter

930754
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

202127
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

930694
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

202151
exponent: 2.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

298040
units: 202053
value: 1.8E-11
type: LocalParameter

202600
stoichiometry: 1.0
species: 930934
type: SpeciesReference

930794
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

931134
notes: This ligand could be any of several which bind to the beta adrenergic receptor and other G-protein coupled receptors which activate AC. For the sake of argument, call it isoproterenol.
constant: true
boundary condition: true
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

203036
stoichiometry: 1.0
species: 930854
type: SpeciesReference

297988
units: 202053
value: 6.0E-11
type: LocalParameter

930734
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

298030
units: 202053
value: 3.6E-11
type: LocalParameter

930834
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

203205
stoichiometry: 1.0
species: 930874
type: SpeciesReference

931394
kinetic law: 202820
product: 202808
reactant: 202795
notes: From Brandt and Ross JBC 261(4):1656-1664, rate is 4/min and is agonist independent. I treat this as irreversible.
fast: false
reversible: true
type: Reaction

202637
stoichiometry: 1.0
species: 930954
type: SpeciesReference

202040
exponent: 1.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

202735
local parameters: 298006
local parameters: 298004
notes: kf*Gs_slash_L_dot_R_dot_GDP_dot_Gabc-kb*Gs_slash_Gbg*Gs_slash_L_dot_R*GTP_dot_Ga
type: KineticLaw

298020
units: 202139
value: 1.99998E-13
type: LocalParameter

202465
stoichiometry: 1.0
species: 930774
type: SpeciesReference

930934
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

202563
stoichiometry: 1.0
species: 930814
type: SpeciesReference

203024
stoichiometry: 1.0
species: 930794
type: SpeciesReference

931374
kinetic law: 202783
product: 202771
reactant: 202759
reactant: 202747
notes: Negligible back-reaction.
fast: false
reversible: true
type: Reaction

202381
stoichiometry: 1.0
species: 930694
type: SpeciesReference

297990
units: 202053
value: 6.0E-11
type: LocalParameter

202662
stoichiometry: 1.0
species: 930974
type: SpeciesReference

930894
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

930554
constant: true
size: 1.0E-12
spatial dimensions: 3.0
type: Compartment

931034
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

931334
kinetic law: 202675
product: 202662
reactant: 202649
reactant: 202637
notes: Ligand binding to receptor. From Gether et al JBC 270:28268-28275 (1995) the binding to the purified receptor is at about 1 uM, but the conformational change only happens at 30 uM. We\'ll take 1 uM for this, since it is already much weaker binding than to the R.Gs complex. The time-course from this paper appears remarkably slow, based on physiological data I estimate more like 10 sec.
fast: false
reversible: true
type: Reaction

931454
kinetic law: 202964
product: 202952
reactant: 202940
reactant: 202928
notes: See Fay et al Biochem 30 5066-5075 1991. kf is 0.01/sec but does not account for Gs levels. kb is 0.0001/sec. The fraction of RG is about 50%, so we can estimate Kd at about the same as for Gs basal levels. This rate has to be faster since it has to feed GTP.Ga into the system faster than the GTPase. Waldhoer et al Mol Pharmacol 53:808-818 1988 say affinity for A1adenosine/Gi is 10 nM.
fast: false
reversible: true
type: Reaction

931354
reactant: 202687
kinetic law: 202735
notes: This step combines several stages in GTP.Galpha release. From Berstein et al activation is at .35 - 0.7/min From Fay et al Biochem 30 5066-5075 1991 kf = .01/sec. From Brandt and Ross JBC 261(4):1656-1664 (1986) and Ransan et al Biochem J 283(2):519-524 (1992) rates around 2.5/min to 1.5/min are better.
reversible: true
product: 202711
product: 202699
fast: false
type: Reaction
product: 202723

930874
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

298022
units: 202053
value: 1.0E-13
type: LocalParameter

202296
local parameters: 297983
local parameters: 297982
notes: kf*PKA_slash_cAMP_dot_R2C2*cAMP-kb*PKA_slash_cAMP2_dot_R2C2
type: KineticLaw

931054
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

298042
units: 202016
value: 20.0
type: LocalParameter

202723
stoichiometry: 1.0
species: 930934
type: SpeciesReference

298032
units: 202053
value: 9.0E-12
type: LocalParameter

297991
units: 202139
value: 1.8E-11
type: LocalParameter

202539
local parameters: 297993
local parameters: 297992
notes: kf*PKA_minus_active*PKA_slash_PKA_minus_inhibitor-kb*PKA_slash_inhibited_minus_PKA
type: KineticLaw

298008
units: 202139
value: 6.0E-12
type: LocalParameter

931014
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

202211
stoichiometry: 1.0
species: 930574
type: SpeciesReference

930974
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

202747
stoichiometry: 1.0
species: 931034
type: SpeciesReference

930594
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.25
initial amount: NaN
in compartment: 930554
type: Species

203254
stoichiometry: 1.0
species: 930754
type: SpeciesReference

203217
stoichiometry: 1.0
species: 930854
type: SpeciesReference

203012
local parameters: 298030
local parameters: 298028
notes: k1*AC_slash_cAMP_minus_PDE*PKA_minus_active-k2*PKA_minus_active_slash_phosph_minus_PDE_slash_phosph_minus_PDE_cplx
type: KineticLaw

202393
local parameters: 297987
local parameters: 297986
notes: kf*cAMP*PKA_slash_cAMP3_dot_R2C2-kb*PKA_slash_cAMP4_dot_R2C2
type: KineticLaw

930574
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.5
initial amount: NaN
in compartment: 930554
type: Species

202477
stoichiometry: 1.0
species: 930734
type: SpeciesReference

298033
units: 202139
value: 2.52E-12
type: LocalParameter

202551
stoichiometry: 1.0
species: 930854
type: SpeciesReference

931194
kinetic law: 202345
product: 202333
reactant: 202321
reactant: 202308
fast: false
reversible: true
type: Reaction

931094
notes: ATP is present in all cells between 2 and 10 mM. See Lehninger. It is assumed buffered since the metabolic activity will take care of its levels.
constant: true
boundary condition: true
has only substance units: false
initial concentration: 5000.0
initial amount: NaN
in compartment: 930554
type: Species

202624
local parameters: 297998
local parameters: 297996
notes: kf*AC_slash_AC*GTP_dot_Ga-kb*AC_slash_Gs_dot_AC
type: KineticLaw

202711
stoichiometry: 1.0
species: 930974
type: SpeciesReference

931074
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 930554
type: Species

931174
kinetic law: 202296
product: 202284
reactant: 202272
reactant: 202260
notes: For now let us set this to the same Km (1e-7M) as site B. This gives kf/kb = .7e-7M * 1e6 / (6e5^2) : 1/(6e5^2) = 2e-13:2.77e-12 Smith et al have better values. They say that this is cooperative, so the consts are now kf/kb =8.3e-4
fast: false
reversible: true
type: Reaction

202675
local parameters: 298002
local parameters: 298000
notes: kf*Gs_slash_R*Gs_slash_L-kb*Gs_slash_L_dot_R
type: KineticLaw

298034
units: 202053
value: 4.0E-11
type: LocalParameter