245788
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.5
initial amount: NaN
in compartment: 245787
type: Species

078553
stoichiometry: 1.0
species: 245927
type: SpeciesReference

078010
stoichiometry: 1.0
species: 245807
type: SpeciesReference

078046
units: 077827
value: 2.31666666666667E-17
type: LocalParameter

078469
units: 077827
value: 4.75E-17
type: LocalParameter

245908
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787
type: Species

078169
stoichiometry: 1.0
species: 245867
type: SpeciesReference

077974
local parameters: 077998
local parameters: 077986
notes: kf*NOS_slash_nNOS*NOS_slash_CaM_minus_Ca4-kb*NOS_slash_Ca_minus_CaMnNOS
type: KineticLaw

078409
units: 077827
value: 1.9E-16
type: LocalParameter

077998
units: 077827
value: 8.33333333333333E-20
type: LocalParameter

245947
notes: CaMKIIalpha caused the most rapid phosphorylation of nNOS, with half-maximal phosphorylation apparent at 3 min and plateau level at 10 min. (Hayashi et al., 1999,JBC,274(29):20597-20602). The Maximal Phosphorylation of nNOS was observed at ~0.4 mol of 32p/mol of nNOS under their conditions.
constant: true
boundary condition: true
has only substance units: false
initial concentration: 1.0
initial amount: NaN
in compartment: 245787
type: Species

078361
stoichiometry: 1.0
species: 245947
type: SpeciesReference

246147
kinetic law: 078589
product: 078577
product: 078565
reactant: 078553
fast: false
reversible: false
type: Reaction

078144
stoichiometry: 1.0
species: 245847
type: SpeciesReference

078445
stoichiometry: 1.0
species: 245947
type: SpeciesReference

246107
kinetic law: 078457
product: 078445
product: 078433
reactant: 078421
fast: false
reversible: false
type: Reaction

245928
notes: Activity is similar to CaMKIalpha, with ~0.7 mol of 32p/mol of nNOS. (Hayashi et 1999,JBC,274(29):20597-20602)
constant: true
boundary condition: true
has only substance units: false
initial concentration: 1.0
initial amount: NaN
in compartment: 245787
type: Species

245907
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787
type: Species

245787
constant: true
size: 1.6667E-18
spatial dimensions: 3.0
type: Compartment

245887
constant: false
boundary condition: false
has only substance units: false
initial concentration: 100.0
initial amount: NaN
in compartment: 245787
type: Species

246067
kinetic law: 078253
product: 078241
reactant: 078229
reactant: 078217
notes: Hayashi et al., 1999, JBC,274(29):20597-20602. and other reported data from sources.
fast: false
reversible: true
type: Reaction

077887
exponent: -1.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

078217
stoichiometry: 1.0
species: 245788
type: SpeciesReference

078107
local parameters: 078132
local parameters: 078120
notes: k1*NOS_slash_Larg*NOS_slash_Ca_minus_CaMnNOS-k2*NOS_slash_Ca_minus_CaMnNOS_slash_kenz_slash_kenz_cplx
type: KineticLaw

078589
local parameters: 078601
notes: k3*NOS_slash_CaMKIalpha_slash_kenz_slash_kenz_cplx
type: KineticLaw

078289
stoichiometry: 1.0
species: 245907
type: SpeciesReference

245968
notes: Phosphorylates nNOS, but not as effective as CaMKIIalpha. (Hayashi et al., 1999,JBC,274(29):20597-20602.) Report of Plateau Level in their phosphorylation plots reaching after 100 min. -- ~0.4 mol of 32p/mol of nNOS.
constant: true
boundary condition: true
has only substance units: false
initial concentration: 1.0
initial amount: NaN
in compartment: 245787
type: Species

077961
stoichiometry: 1.0
species: 245827
type: SpeciesReference

078313
stoichiometry: 1.0
species: 245928
type: SpeciesReference

246047
kinetic law: 078193
product: 078181
product: 078169
product: 078156
reactant: 078144
fast: false
reversible: false
type: Reaction

078193
local parameters: 078205
notes: k3*NOS_slash_Ca_minus_CaMnNOS_slash_kenz_slash_kenz_cplx
type: KineticLaw

078481
stoichiometry: 1.0
species: 245788
type: SpeciesReference

078517
local parameters: 078541
local parameters: 078529
notes: k1*NOS_slash_nNOS*NOS_slash_CaMKIalpha-k2*NOS_slash_CaMKIalpha_slash_kenz_slash_kenz_cplx
type: KineticLaw

245849
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787
type: Species

078120
units: 077863
value: 1.38891666666667E-17
type: LocalParameter

078265
units: 077863
value: 3.0E-17
type: LocalParameter

245927
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787
type: Species

078094
stoichiometry: 1.0
species: 245847
type: SpeciesReference

245867
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787
type: Species

078337
units: 077827
value: 3.0E-17
type: LocalParameter

077936
stoichiometry: 1.0
species: 245788
type: SpeciesReference

078565
stoichiometry: 1.0
species: 245807
type: SpeciesReference

245772
constant: true
size: 1.0E-18
spatial dimensions: 3.0
type: Compartment

077924
exponent: 1.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

078070
stoichiometry: 1.0
species: 245887
type: SpeciesReference

077986
units: 077863
value: 5.41666666666667E-18
type: LocalParameter

078601
units: 077827
value: 2.83333333333333E-17
type: LocalParameter

078349
stoichiometry: 1.0
species: 245788
type: SpeciesReference

078034
local parameters: 078058
local parameters: 078046
notes: kf*NOS_slash_NOS_star_-kb*NOS_slash_nNOS
type: KineticLaw

078541
units: 077827
value: 1.13333333333333E-16
type: LocalParameter

078529
units: 077863
value: 2.83333333333333E-17
type: LocalParameter

078022
stoichiometry: 1.0
species: 245788
type: SpeciesReference

245987
constant: true
boundary condition: true
has only substance units: false
initial concentration: 20.0
initial amount: NaN
in compartment: 245787
type: Species

078457
local parameters: 078469
notes: k3*NOS_slash_CaMKIIalpha_slash_kenz_slash_kenz_cplx
type: KineticLaw

078253
local parameters: 078277
local parameters: 078265
notes: k1*NOS_slash_nNOS*NOS_slash_CaMKIV-k2*NOS_slash_CaMKIV_slash_kenz_slash_kenz_cplx
type: KineticLaw

MODEL4780784080
species: 245849
substance units: 077912
species: 245907
reaction: 246087
species: 245807
units def: 077912
is described by: http://identifiers.org/pubmed/10400690
reaction: 246090
species: 245968
reaction: 246007
length unit: length
compartment: 245772
species: 245788
volume units: metaid_0000217
species: 245827
reaction: 246127
units def: 077863
compartment: 245787
species: 245947
reaction: 245988
reaction: 246147
name: Hayashi1999_NOSynth_Phospho
species: 245847
time units: 013121
species: 245867
reaction: 246027
species: 245927
species: 245987
species: 245887
reaction: 246067
area units: area
reaction: 246047
curated: false
species: 245908
units def: 077827
species: 245928
reaction: 246107
notes: This model features the phosphorylation of rat brain neuronal NOS expressed in E. coli or Sf9 cells, which leads to a decrease in Vmax of the phosphorylated enzyme, with little change of both the Km for L-arginine and Kact for CaM. This is based on Hayashi Y. et al. J Biol Chem. (1999) 274(29):20597-602. They report of phosphorylatin being carried out by CaM kinases I alpha, II alpha and IV. The rates used have been obtained from their paper and from other reported data. This model originates from BioModels Database: A Database of Annotated Published Models (http://www.ebi.ac.uk/biomodels/). It is copyright (c) 2005-2011 The BioModels.net Team. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not.. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.
type: SBMLModel

245807
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787
type: Species

078205
units: 077827
value: 2.77783333333333E-17
type: LocalParameter

077899
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

246090
kinetic law: 078385
product: 078373
reactant: 078361
reactant: 078349
notes: Hayashi et al., 1999, JBC,274(29):20597-20602. and from various other datas.
fast: false
reversible: true
type: Reaction

078397
units: 077863
value: 4.75E-17
type: LocalParameter

077949
stoichiometry: 1.0
species: 245987
type: SpeciesReference

078433
stoichiometry: 1.0
species: 245807
type: SpeciesReference

078058
units: 077827
value: 0.0
type: LocalParameter

078156
stoichiometry: 1.0
species: 245849
type: SpeciesReference

078373
stoichiometry: 1.0
species: 245908
type: SpeciesReference

246087
kinetic law: 078325
product: 078313
product: 078301
reactant: 078289
fast: false
reversible: false
type: Reaction

078277
units: 077827
value: 1.2E-16
type: LocalParameter

078301
stoichiometry: 1.0
species: 245807
type: SpeciesReference

245827
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787
type: Species

078385
local parameters: 078409
local parameters: 078397
notes: k1*NOS_slash_nNOS*NOS_slash_CaMKIIalpha-k2*NOS_slash_CaMKIIalpha_slash_kenz_slash_kenz_cplx
type: KineticLaw

246027
kinetic law: 078107
product: 078094
reactant: 078082
reactant: 078070
notes: Km for purified NOS is estimated between 1 - 10 uM. (Prog in Neurobiology, 2001, 64: 365-391) Vmax for unphosporylated NOS, the active form, is 500-1500 nmol/nmol/min (Montellano et al., 1998, JBC,26(12): 1185-1189). Hayashi et al., JBC, 1999, 274(29):20597-20602 report Vmax (nmol/min/mg) of nNOS Unphosporylated at 95.7 (+-) 4.2
fast: false
reversible: true
type: Reaction

078421
stoichiometry: 1.0
species: 245908
type: SpeciesReference

078493
stoichiometry: 1.0
species: 245968
type: SpeciesReference

246127
kinetic law: 078517
product: 078505
reactant: 078493
reactant: 078481
notes: enzyme parameters used from different literature. Hayashi et al., JBC,274(29):20597-20602.
fast: false
reversible: true
type: Reaction

077875
exponent: 2.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

245988
kinetic law: 077974
product: 077961
reactant: 077949
reactant: 077936
notes: Those binding CaM have a high Kd, including nNOS, ~<=10nM. The binding of CaM to nNOS has been demonstrated to act as the trigger necessary for electron transfer and catalytic activity. (Marletta, 1997;36:12337-12345).
fast: false
reversible: true
type: Reaction

078229
stoichiometry: 1.0
species: 245928
type: SpeciesReference

078577
stoichiometry: 1.0
species: 245968
type: SpeciesReference

078132
units: 077827
value: 1.11113333333333E-16
type: LocalParameter

078325
local parameters: 078337
notes: k3*NOS_slash_CaMKIV_slash_kenz_slash_kenz_cplx
type: KineticLaw

078241
stoichiometry: 1.0
species: 245907
type: SpeciesReference

078082
stoichiometry: 1.0
species: 245827
type: SpeciesReference

078505
stoichiometry: 1.0
species: 245927
type: SpeciesReference

245847
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 245787
type: Species

078181
stoichiometry: 1.0
species: 245827
type: SpeciesReference

077851
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

246007
kinetic law: 078034
product: 078022
reactant: 078010
notes: kf -13.9 These rates used to keep the basal level of nNOS at reasonable levels.
fast: false
reversible: true
type: Reaction

077839
exponent: 1.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit