ATP:D-fructose-6-phosphate 1-phosphotransferase
5,10-Methylenetetrahydrofolate:NADP+ oxidoreductase
D-Glyceraldehyde-3-phosphate:NAD+ oxidoreductase(phosphorylating)
2-Deoxy-D-ribose-5-phosphate acetaldehyde-lyase
GENE ASSOCIATION: MPN494 and MPN495 and MPN496
UTP:alpha-D-glucose-1-phosphate uridylyltransferase
ATP:pantetheine-4'-phosphate adenylyltransferase
UMP:pyrophosphate phosphoribosyltransferase
GENE ASSOCIATION: MPN322 and MPN323 and MPN324
dUMP + H2O >=< Deoxyuridine + Orthophosphate
L-leucine reversible transport via proton symport (R)
GTP:3-phospho-D-glycerate 1-phosphotransferase
Adenosine 3',5'-bisphosphate 3'(2')-phosphohydrolase
GENE ASSOCIATION: MPN434 and MPN002 and MPN120
ATP:D-ribose-5-phosphate pyrophosphotransferase
ATP:deoxyadenosine 5'-phosphotransferase
2'-Deoxycytidine 5'-monophosphate phosphohydrolase
Sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphateglycolaldehyde transferase
GENE ASSOCIATION: MPN236 and MPN237 and MPN238
Wodke2013 - Genome-scale constraint-based model of M.pneumoniae energy metabolism (iJW145) A new genome-scale metabolic reconstruction of M.pneumoniae is used in combination with external metabolite measurement and protein abundance measurements to quantitatively explore the energy metabolism of this genome-reduce human pathogen. This model is described in the article: Dissecting the energy metabolism in Mycoplasma pneumoniae through genome-scale metabolic modeling. Wodke JA, Puchałka J, Lluch-Senar M, Marcos J, Yus E, Godinho M, Gutiérrez-Gallego R, dos Santos VA, Serrano L, Klipp E, Maier T. Mol Syst Biol. 2013;9:653. Abstract: Mycoplasma pneumoniae, a threatening pathogen with a minimal genome, is a model organism for bacterial systems biology for which substantial experimental information is available. With the goal of understanding the complex interactions underlying its metabolism, we analyzed and characterized the metabolic network of M. pneumoniae in great detail, integrating data from different omics analyses under a range of conditions into a constraint-based model backbone. Iterating model predictions, hypothesis generation, experimental testing, and model refinement, we accurately curated the network and quantitatively explored the energy metabolism. In contrast to other bacteria, M. pneumoniae uses most of its energy for maintenance tasks instead of growth. We show that in highly linear networks the prediction of flux distributions for different growth times allows analysis of time-dependent changes, albeit using a static model. By performing an in silico knock-out study as well as analyzing flux distributions in single and double mutant phenotypes, we demonstrated that the model accurately represents the metabolism of M. pneumoniae. The experimentally validated model provides a solid basis for understanding its metabolic regulatory mechanisms. This model is hosted on BioModels Database and identified by: MODEL1301290000 . To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models . To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.
Wodke2013 - Genome-scale constraint-based model of M.pneumoniae energy metabolism (iJW145)
Carbamoyl-phosphate:L-ornithine carbamoyltransferase
Glycerone phosphate phosphohydrolase (alkaline optimum)
Deoxyadenosine:orthophosphate ribosyltransferase
Acetyl-CoA:dihydrolipoamide S-acetyltransferase
ATP:nicotinamide-nucleotide adenylyltransferase
Acetyl-CoA:orthophosphate acetyltransferase
5-Formyltetrahydrofolate cyclo-ligase (ADP-forming)
D-Mannitol-1-phosphate:NAD+ 5-oxidoreductase
sn-Glycerol-3-phosphate:oxygen 2-oxidoreductase
Thymidine:orthophosphate deoxyribosyltransferase
Uridine 5'-monophosphate phosphohydrolase
GENE ASSOCIATION: (MPN304 and MPN305) or MPN560
Nicotinate D-ribonucleotide:pyrophosphate phosphoribosyltransferase
ATP:thiamin-phosphate phosphotransferase
Guanosine:orthophosphate ribosyltransferase
L-tyrosine reversible transport via proton symport (R)
Adenosine 5'-monophosphate phosphohydrolase
L-asparagine reversible transport via proton symport (R)
2-oxoglutarate dehydrogenase, lipoamidedehydrogenase compone
1-acyl-sn-glycerol-3-phosphate acyltransferase
Deamino-NAD+:ammonia ligase (AMP-forming)
Adenosine:orthophosphate ribosyltransferase
D-Fructose 1-phosphate D-glyceraldehyde-3-phosphate-lyase
ATP:L-methione S-adenosyltransferase (R)
Pyruvate:lipoamide 2-oxidoreductase(decarboxylating andacceptor-acetylating)
L-histidine reversible transport via proton symport (R)
2-Deoxy-D-ribose 1-phosphate 1,5-phosphomutase
3-keto-L-gulonate 6-phosphate decarboxylase
GENE ASSOCIATION: MPN258 and MPN259 and MPN260
Guanosine 5'-monophosphate phosphohydrolase
Uridine:orthophosphate ribosyltransferase
GENE ASSOCIATION: MPN609 and MPN610 and MPN611
ATP:3-phospho-D-glycerate 1-phosphotransferase
glycerol-3-phosphate O-acyltransferase (Mpn)
AMP:pyrophosphate phosphoribosyltransferase
GENE ASSOCIATION: MPN133 and MPN134 and MPN135 and MPN136
GMP:pyrophosphate phosphoribosyltransferase
GENE ASSOCIATION: MPN597 and MPN598 and MPN599 and MPN600 and MPN601 and MPN602 and MPN603 and MPN604
Cytidine-5'-monophosphate phosphohydrolase
dATP:3-phospho-D-glycerate 1-phosphotransferase
5,6,7,8-Tetrahydrofolate:NAD+ oxidoreductase
Deoxyguanosine:orthophosphate ribosyltransferase
Acetaldehyde:NAD+ oxidoreductase (CoA-acetylating)
D-Glyceraldehyde-3-phosphate ketol-isomerase
5,10-Methylenetetrahydrofolate:dUMP C-methyltransferase
CTP:choline-phosphate cytidylyltransferase
glycine reversible transport via proton symport (R)
5,10-Methenyltetrahydrofolate 5-hydrolase(decyclizing)
ATP:deoxyguanosine 5'-phosphotransferase
S-Aminomethyldihydrolipoylprotein:(6S)-tetrahydrofolateaminomethyltransferase (ammonia-forming) (R)
sn-Glycero-3-phosphocholine glycerophosphohydrolase
cytidine transport in via proton symport, reversible
Deoxyuridine:orthophosphate ribosyltransferase
L-tryptophan reversible transport via proton symport (R)
5,10-Methylenetetrahydrofolate:glycine hydroxymethyltransferase
D-Fructose-1,6-bisphosphate D-glyceraldehyde-3-phosphate-lyase
Formate:tetrahydrofolate ligase (ADP-forming)
Phosphatidylcholine ABC transporter (Mpn)
dGTP:3-phospho-D-glycerate 1-phosphotransferase