CDMT00029
stoichiometry: 1.0
species: s25
type: SpeciesReference

substance
unit: CDMT00005
name: substance
type: SBMLUnitsDef

CDMT00042
stoichiometry: 1.0
species: s8
type: SpeciesReference

CDMT00015
exponent: 1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

khydB4T
constant: true
units: ps
value: 2.0
type: Parameter

re59
kinetic law: CDMT00173
product: CDMT00004
reactant: CDMT00003
reactant: CDMT00002
fast: false
reversible: true
name: R3: Rev. SPB-association of Cdc5-phosphorylated Bfa1
type: Reaction

CDMT00102
stoichiometry: 1.0
species: s3
type: SpeciesReference

W3 CDTF: Tue Mar 06 17:01:30 GMT 2012

re19
kinetic law: CDMT00145
product: CDMT00047
reactant: CDMT00046
reactant: CDMT00045
fast: false
reversible: true
name: R10: Rev. association of Tem1GTP with SPB-bound Bfa1
type: Reaction

CDMT00181
persistant: true
initial value: true
type: Trigger

re29
kinetic law: CDMT00153
product: CDMT00069
reactant: CDMT00068
reactant: CDMT00067
fast: false
reversible: true
name: R19: Rev. association of Tem1GDP with Bfa1
type: Reaction

s8
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 4.91E-8
initial amount: NaN
in compartment: c2
name: Tem1GTP
type: Species

CDMT00001
exponent: 1.0
scale: 0
multipler: 1.0
kind: DIMENSIONLESS
type: SBMLUnit

s41
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1P4-Tem1GDP
type: Species

CDMT00116
stoichiometry: 1.0
species: s65
type: SpeciesReference

CDMT00112
stoichiometry: 1.0
species: s22
type: SpeciesReference

CDMT00056
stoichiometry: 1.0
species: s1
type: SpeciesReference

CDMT00041
stoichiometry: 1.0
species: s56
type: SpeciesReference

konT
constant: true
units: pmps
value: 1900000.0
type: Parameter

CDMT00016
stoichiometry: 1.0
species: s10
type: SpeciesReference

total Bfa1 SPB
constant: false
units: molecules
value: 0.0
name: Total Bfa1 at the SPB
type: Parameter

CDMT00069
stoichiometry: 1.0
species: s59
type: SpeciesReference

CDMT00103
stoichiometry: 1.0
species: s4
type: SpeciesReference

W3 CDTF: Thu Feb 09 13:50:30 GMT 2012

s37
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1-Tem1GDP
type: Species

CDMT00180
exponent: -1.0
scale: 0
multipler: 1.0
kind: MOLE
type: SBMLUnit

s7
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1P5
type: Species

CDMT00002
stoichiometry: 1.0
species: s7
type: SpeciesReference

CDMT00055
stoichiometry: 1.0
species: s62
type: SpeciesReference

total Tem1 SPB
constant: false
units: molecules
value: 0.0
name: Total Tem1 at the SPB
type: Parameter

CDMT00113
stoichiometry: 1.0
species: s19
type: SpeciesReference

CDMT00117
stoichiometry: 1.0
species: s59
type: SpeciesReference

c2
constant: true
units: litre
size: 1.0E-13
spatial dimensions: 3.0
name: Cytosol
type: Compartment

konB
constant: true
units: pmps
value: 1250000.0
type: Parameter

CDMT00027
stoichiometry: 1.0
species: s1
type: SpeciesReference

CDMT00040
stoichiometry: 1.0
species: s8
type: SpeciesReference

CDMT00118
stoichiometry: 1.0
species: s59
type: SpeciesReference

ORG:
  orgname: Deutsches Krebsforschungszentrum Heidelberg
n:
  given: Maiko
  family: Lohel

re8
kinetic law: CDMT00135
product: CDMT00019
reactant: CDMT00018
fast: false
reversible: false
name: R27: Phosphorylation of SPB-bound Bfa1 by Cdc5
type: Reaction

konB4T
constant: true
units: pmps
value: 3.65E7
type: Parameter

s10
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 8.33E-5
initial amount: NaN
in compartment: c3
name: B-Bfa1
type: Species

CDMT00017
stoichiometry: 1.0
species: s13
type: SpeciesReference

CDMT00030
stoichiometry: 1.0
species: s29
type: SpeciesReference

re57
kinetic law: CDMT00171
product: CDMT00125
reactant: CDMT00124
fast: false
reversible: false
name: R43: GAP-accelerated GTP-hydrolysis
type: Reaction

CDMT00068
stoichiometry: 1.0
species: s9
type: SpeciesReference

CDMT00128
exponent: -1.0
scale: 0
multipler: 1.0
kind: MOLE
type: SBMLUnit

s53
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1P4-Tem1GTP
type: Species

CDMT00100
stoichiometry: 1.0
species: s62
type: SpeciesReference

CDMT00058
stoichiometry: 1.0
species: s59
type: SpeciesReference

CDMT00114
stoichiometry: 1.0
species: s19
type: SpeciesReference

CDMT00028
stoichiometry: 1.0
species: s33
type: SpeciesReference

knex
constant: true
units: ps
value: 0.0136
type: Parameter

CDMT00119
stoichiometry: 1.0
species: s62
type: SpeciesReference

re9
kinetic law: CDMT00136
product: CDMT00022
reactant: CDMT00021
reactant: CDMT00020
fast: false
reversible: true
name: R5: Rev. SPB-association of Kin4-phosphorylated Bfa1-Tem1GTP complex
type: Reaction

re30
kinetic law: CDMT00154
product: CDMT00072
reactant: CDMT00071
reactant: CDMT00070
fast: false
reversible: true
name: R21: Rev. association of Tem1GDP with Cdc5-phosphorylated Bfa1
type: Reaction

CDMT00129
exponent: 1.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

total Tem1 Cytosol
constant: false
units: molecules
value: 0.0
name: Total Tem1 in the Cytosol
type: Parameter

CDMT00099
stoichiometry: 1.0
species: s53
type: SpeciesReference

re58
kinetic law: CDMT00172
product: CDMT00127
reactant: CDMT00126
fast: false
reversible: false
name: R45: GTP-hydrolysis with intrinisc GTPase activity
type: Reaction

CDMT00018
stoichiometry: 1.0
species: s10
type: SpeciesReference

re18
kinetic law: CDMT00144
product: CDMT00044
reactant: CDMT00043
reactant: CDMT00042
fast: false
reversible: true
name: R11: Rev. association of Tem1GTP with SPB-bound Kin4-phosphorylated Bfa1
type: Reaction

CDMT00079
stoichiometry: 1.0
species: s41
type: SpeciesReference

active Bfa1 SPB
constant: false
units: molecules
value: 0.0
name: Active Bfa1 at the SPB
type: Parameter

ps
unit: CDMT00131
name: per second
type: SBMLUnitsDef

pmole
unit: CDMT00180
name: per mole
type: SBMLUnitsDef

CDMT00067
stoichiometry: 1.0
species: s3
type: SpeciesReference

krKin4
constant: true
units: ps
value: 0.0251
type: Parameter

CDMT00101
stoichiometry: 1.0
species: s59
type: SpeciesReference

alpha
constant: true
units: dimensionless
value: 1.0
name: alpha
type: Parameter

s9
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 7.99E-9
initial amount: NaN
in compartment: c2
name: Tem1GDP
type: Species

re44
kinetic law: CDMT00161
product: CDMT00094
reactant: CDMT00093
reactant: CDMT00092
fast: false
reversible: true
name: R23: Rev. SPB-association of Tem1GDP
type: Reaction

CDMT00057
stoichiometry: 1.0
species: s41
type: SpeciesReference

CDMT00115
stoichiometry: 1.0
species: s22
type: SpeciesReference

s62
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1P4-Tem1GDP
type: Species

s25
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1-Tem1GTP
type: Species

CDMT00046
stoichiometry: 1.0
species: s10
type: SpeciesReference

re55
kinetic law: CDMT00169
product: CDMT00115
reactant: CDMT00114
fast: false
reversible: false
name: R41: GTP-hydrolysis with intrinisc GTPase activity
type: Reaction

CDMT00032
stoichiometry: 1.0
species: s33
type: SpeciesReference

CDMT00011
stoichiometry: 1.0
species: s3
type: SpeciesReference

CDMT00025
stoichiometry: 1.0
species: s25
type: SpeciesReference

re65
kinetic law: CDMT00177
product: CDMT00101
reactant: CDMT00100
fast: false
reversible: false
name: R35: Dephosphorylation of Bfa1 by a yet unidentified phosphatase counteracting Kin4
type: Reaction

s3
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 2.03E-8
initial amount: NaN
in compartment: c2
name: Bfa1
type: Species

s65
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1P5-Tem1GDP
type: Species

CDMT00088
stoichiometry: 1.0
species: s25
type: SpeciesReference

re15
kinetic law: CDMT00142
product: CDMT00038
reactant: CDMT00037
reactant: CDMT00036
fast: false
reversible: true
name: R16: Rev. association of Tem1GTP with Bfa1
type: Reaction

active Tem1 Cytosol
constant: false
units: molecules
value: 0.0
name: Active Tem1 in the cytosol
type: Parameter

s22
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: T-Tem1GDP
type: Species

konB4
constant: true
units: pmps
value: 20000.0
type: Parameter

CDMT00005
exponent: 1.0
scale: 0
multipler: 1.0
kind: MOLE
type: SBMLUnit

CDMT00131
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

CDMT00052
stoichiometry: 1.0
species: s41
type: SpeciesReference

re35
kinetic law: CDMT00156
product: CDMT00082
reactant: CDMT00081
reactant: CDMT00080
fast: false
reversible: true
name: R13: Rev. association of Tem1GDP with SPB-bound Bfa1
type: Reaction

CDMT00066
stoichiometry: 1.0
species: s62
type: SpeciesReference

re25
kinetic law: CDMT00150
product: CDMT00060
reactant: CDMT00059
reactant: CDMT00058
fast: false
reversible: true
name: R7: Rev. SPB-association of Bfa1-Tem1GDP complex
type: Reaction

CDMT00078
stoichiometry: 1.0
species: s13
type: SpeciesReference

re56
kinetic law: CDMT00170
product: CDMT00123
reactant: CDMT00122
fast: false
reversible: false
name: R44: GAP-accelerated GTP-hydrolysis
type: Reaction

CDMT00045
stoichiometry: 1.0
species: s8
type: SpeciesReference

CDMT00031
stoichiometry: 1.0
species: s25
type: SpeciesReference

s50
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1-Tem1GTP
type: Species

s13
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1P4
type: Species

CDMT00012
stoichiometry: 1.0
species: s10
type: SpeciesReference

length
unit: CDMT00014
name: length
type: SBMLUnitsDef

MODEL1202090003
reaction: re65
parameter: inactive Tem1 Cytosol
reaction: re50
units def: area
rule: CDMT00186
reaction: re14
species: s1
parameter: koffB
time units: time
reaction: re44
length unit: length
reaction: re63
reaction: re36
notes: This model is from the article: A dynamical model of the spindle position checkpoint Ayse Koca Caydasi, Maiko Lohel, Gerd Grünert, Peter Dittrich, Gislene Pereira & Bashar Ibrahim Molecular Systems Biology 2012; 582 PMEDID , doi: 10.1038/msb.2012.15 Abstract: The orientation of the mitotic spindle with respect to the polarity axis is crucial for the accuracy of asymmetric cell division. In budding yeast, a surveillance mechanism called the spindle position checkpoint (SPOC) prevents exit from mitosis when the mitotic spindle fails to align along the mother-to-daughter polarity axis. SPOC arrest relies upon inhibition of the GTPase Tem1 by the GTPase-activating protein (GAP) complex Bfa1–Bub2. Importantly, reactions signaling mitotic exit take place at yeast centrosomes (named spindle pole bodies, SPBs) and the GAP complex also promotes SPB localization of Tem1. Yet, whether the regulation of Tem1 by Bfa1–Bub2 takes place only at the SPBs remains elusive. Here, we present a quantitative analysis of Bfa1–Bub2 and Tem1 localization at the SPBs. Based on the measured SPB-bound protein levels, we introduce a dynamical model of the SPOC that describes the regulation of Bfa1 and Tem1. Our model suggests that Bfa1 interacts with Tem1 in the cytoplasm as well as at the SPBs to provide efficient Tem1 inhibition. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanc…
species: s16
species: s7
parameter: total Tem1 Cytosol
species: s33
substance units: substance
rule: CDMT00192
rule: CDMT00194
parameter: khydBT
parameter: krCdc5
parameter: konB
units def: pmole
species: s50
volume units: volume
parameter: krKin4
reaction: re34
species: s10
curated: false
species: s65
reaction: re4
reaction: re8
parameter: khydB4T
parameter: koffBT
reaction: re18
units def: substance
parameter: kfKin4Cyto
compartment: c2
reaction: re10
compartment: default
rule: CDMT00191
reaction: re54
species: s41
reaction: re23
parameter: konBT
species: s37
reaction: re60
parameter: active Bfa1 SPB
reaction: re67
reaction: re52
parameter: NA
reaction: re59
species: s3
reaction: re16
species: s2
parameter: knex
units def: ps
species: s8
species: s13
species: s59
units def: time
reaction: re57
parameter: total Tem1 SPB
reaction: re26
rule: CDMT00193
parameter: koffB4
parameter: konT
species: s45
parameter: inactive Bfa1 SPB
units def: molar
parameter: inactive Tem1 SPB
reaction: re30
event: spindle alignment
parameter: total Bfa1 SPB
parameter: konB4T
reaction: re12
reaction: re46
parameter: konB5T

CDMT00026
stoichiometry: 1.0
species: s56
type: SpeciesReference

re66
kinetic law: CDMT00178
product: CDMT00117
reactant: CDMT00116
fast: false
reversible: false
name: R38: Dephosphorylation of Cdc5-phosphorylated Bfa1 presumably by PP2A
type: Reaction

s2
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 1.66E-4
initial amount: NaN
in compartment: c3
name: T
type: Species

re16
kinetic law: CDMT00143
product: CDMT00041
reactant: CDMT00040
reactant: CDMT00039
fast: false
reversible: true
name: R18: Rev. association of Tem1GTP with Cdc5-phosphorylated Bfa1
type: Reaction

CDMT00130
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

re46
kinetic law: CDMT00162
product: CDMT00097
reactant: CDMT00096
reactant: CDMT00095
fast: false
reversible: true
name: R22: Rev. SPB-association of Tem1GTP
type: Reaction

CDMT00065
stoichiometry: 1.0
species: s9
type: SpeciesReference

CDMT00006
exponent: 1.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

CDMT00051
stoichiometry: 1.0
species: s37
type: SpeciesReference

s33
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1P5-Tem1GTP
type: Species

inactive Bfa1 SPB
constant: false
units: molecules
value: 0.0
name: Inactive Bfa1 at the SPB
type: Parameter

re26
kinetic law: CDMT00151
product: CDMT00063
reactant: CDMT00062
reactant: CDMT00061
fast: false
reversible: true
name: R9: Rev. SPB-association of Cdc5-phosphorylated Bfa1-Tem1GDP complex
type: Reaction

CDMT00077
stoichiometry: 1.0
species: s9
type: SpeciesReference

re36
kinetic law: CDMT00157
product: CDMT00085
reactant: CDMT00084
reactant: CDMT00083
fast: false
reversible: true
name: R15: Rev. association of Tem1GDP with SPB-bound Cdc5-phosphorylated Bfa1
type: Reaction

CDMT00087
stoichiometry: 1.0
species: s41
type: SpeciesReference

CDMT00013
exponent: 2.0
scale: 0
multipler: 1.0
kind: METRE
type: SBMLUnit

CDMT00044
stoichiometry: 1.0
species: s29
type: SpeciesReference

total Bfa1 Cytosol
constant: false
units: molecules
value: 0.0
name: Total Bfa1 in the Cytosol
type: Parameter

CDMT00034
stoichiometry: 1.0
species: s8
type: SpeciesReference

re67
kinetic law: CDMT00179
product: CDMT00119
reactant: CDMT00118
fast: false
reversible: false
name: R32: Phosphorylation of Bfa1:Tem1GDP by cytosolic Kin4
type: Reaction

CDMT00023
stoichiometry: 1.0
species: s50
type: SpeciesReference

CDMT00064
stoichiometry: 1.0
species: s4
type: SpeciesReference

re13
kinetic law: CDMT00140
product: CDMT00032
reactant: CDMT00031
fast: false
reversible: false
name: R28: Phosphorylation of SPB-bound Bfa1:Tem1GTP by Cdc5
type: Reaction

CDMT00110
stoichiometry: 1.0
species: s8
type: SpeciesReference

CDMT00076
stoichiometry: 1.0
species: s50
type: SpeciesReference

CDMT00003
stoichiometry: 1.0
species: s1
type: SpeciesReference

volume
unit: CDMT00006
name: volume
type: SBMLUnitsDef

re53
kinetic law: CDMT00167
product: CDMT00111
reactant: CDMT00110
fast: false
reversible: false
name: R39: GTP-hydrolysis with intrinisc GTPase activity
type: Reaction

CDMT00054
stoichiometry: 1.0
species: s45
type: SpeciesReference

CDMT00014
exponent: 1.0
scale: 0
multipler: 1.0
kind: METRE
type: SBMLUnit

inactive Bfa1 Cytosol
constant: false
units: molecules
value: 0.0
name: Inactive Bfa1 in the cytosol
type: Parameter

re54
kinetic law: CDMT00168
product: CDMT00113
reactant: CDMT00112
fast: false
reversible: false
name: R42: GDP- for GTP nucleotide exchange
type: Reaction

CDMT00033
stoichiometry: 1.0
species: s4
type: SpeciesReference

s4
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1P4
type: Species

CDMT00043
stoichiometry: 1.0
species: s13
type: SpeciesReference

CDMT00024
stoichiometry: 1.0
species: s1
type: SpeciesReference

ORG:
  orgname: Deutsches Krebsforschungszentrum Heidelberg
n:
  given: Maiko
  family: Lohel

CDMT00063
stoichiometry: 1.0
species: s45
type: SpeciesReference

CDMT00111
stoichiometry: 1.0
species: s9
type: SpeciesReference

CDMT00004
stoichiometry: 1.0
species: s16
type: SpeciesReference

re14
kinetic law: CDMT00141
product: CDMT00035
reactant: CDMT00034
reactant: CDMT00033
fast: false
reversible: true
name: R17: Rev. association of Tem1GTP with Kin4-phosphorylated Bfa1
type: Reaction

CDMT00089
stoichiometry: 1.0
species: s37
type: SpeciesReference

krCdc5
constant: true
units: ps
value: 0.01
type: Parameter

CDMT00075
stoichiometry: 1.0
species: s56
type: SpeciesReference

re28
kinetic law: CDMT00152
product: CDMT00066
reactant: CDMT00065
reactant: CDMT00064
fast: false
reversible: true
name: R20: Rev. association of Tem1GDP with Kin4-phosphorylated Bfa1
type: Reaction

re34
kinetic law: CDMT00155
product: CDMT00079
reactant: CDMT00078
reactant: CDMT00077
fast: false
reversible: true
name: R14: Rev. association of Tem1GDP with SPB-bound Kin4-phosphorylated Bfa1
type: Reaction

CDMT00053
stoichiometry: 1.0
species: s37
type: SpeciesReference

CDMT00083
stoichiometry: 1.0
species: s9
type: SpeciesReference

re51
kinetic law: CDMT00165
product: CDMT00107
reactant: CDMT00106
fast: false
reversible: false
name: R36: Dephosphorylation of Cdc5-phosphorylated Bfa1 presumably by PP2A
type: Reaction

re22
kinetic law: CDMT00147
product: CDMT00052
reactant: CDMT00051
fast: false
reversible: false
name: R26: Phosphorylation of SPB-bound Bfa1:Tem1GDP by Kin4
type: Reaction

inactive Tem1 Cytosol
constant: false
units: molecules
value: 0.0
name: Inactive Tem1 in the cytosol
type: Parameter

active Tem1 SPB
constant: false
units: molecules
value: 0.0
name: Active Tem1 at the SPB
type: Parameter

re12
kinetic law: CDMT00139
product: CDMT00030
reactant: CDMT00029
fast: false
reversible: false
name: R25: Phosphorylation of SPB-bound Bfa1:Tem1GTP by Kin4
type: Reaction

re61
kinetic law: CDMT00175
product: CDMT00076
reactant: CDMT00075
fast: false
reversible: false
name: R37: Dephosphorylation of Cdc5-phosphorylated Bfa1 presumably by PP2A
type: Reaction

CDMT00061
stoichiometry: 1.0
species: s65
type: SpeciesReference

CDMT00073
stoichiometry: 1.0
species: s53
type: SpeciesReference

CDMT00121
exponent: -1.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

CDMT00109
stoichiometry: 1.0
species: s8
type: SpeciesReference

default
constant: true
units: litre
size: 1.0
spatial dimensions: 3.0
type: Compartment

CDMT00093
stoichiometry: 1.0
species: s2
type: SpeciesReference

CDMT00036
stoichiometry: 1.0
species: s3
type: SpeciesReference

CDMT00021
stoichiometry: 1.0
species: s1
type: SpeciesReference

re39
kinetic law: CDMT00159
product: CDMT00089
reactant: CDMT00088
fast: false
reversible: false
name: R46: GAP-accelerated GTP-hydrolysis
type: Reaction

CDMT00084
stoichiometry: 1.0
species: s16
type: SpeciesReference

CDMT00120
exponent: 1.0
scale: 0
multipler: 1.0
kind: MOLE
type: SBMLUnit

re21
kinetic law: CDMT00146
product: CDMT00050
reactant: CDMT00049
reactant: CDMT00048
fast: false
reversible: true
name: R12: Rev. association of Tem1GTP with SPB-bound Cdc5-phosphorylated Bfa1
type: Reaction

re52
kinetic law: CDMT00166
product: CDMT00109
reactant: CDMT00108
fast: false
reversible: false
name: R40: GDP- for GTP nucleotide exchange
type: Reaction

CDMT00062
stoichiometry: 1.0
species: s1
type: SpeciesReference

re11
kinetic law: CDMT00138
product: CDMT00028
reactant: CDMT00027
reactant: CDMT00026
fast: false
reversible: true
name: R6: Rev. SPB-association of Cdc5-phosphorylated Bfa1-Tem1GTP complex
type: Reaction

CDMT00074
stoichiometry: 1.0
species: s50
type: SpeciesReference

re49
kinetic law: CDMT00163
product: CDMT00103
reactant: CDMT00102
fast: false
reversible: false
name: R30: Phosphorylation of Bfa1 by cytosolic Kin4
type: Reaction

CDMT00108
stoichiometry: 1.0
species: s9
type: SpeciesReference

CDMT00094
stoichiometry: 1.0
species: s22
type: SpeciesReference

CDMT00022
stoichiometry: 1.0
species: s29
type: SpeciesReference

s16
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1P5
type: Species

molecules
unit: CDMT00001
name: molecules
type: SBMLUnitsDef

CDMT00035
stoichiometry: 1.0
species: s53
type: SpeciesReference

molar
unit: CDMT00121
unit: CDMT00120
name: molar
type: SBMLUnitsDef

CDMT00049
stoichiometry: 1.0
species: s16
type: SpeciesReference

re24
kinetic law: CDMT00149
product: CDMT00057
reactant: CDMT00056
reactant: CDMT00055
fast: false
reversible: true
name: R8: Rev. SPB-association of Kin4 phosphorylated Bfa1-Tem1GDP complex
type: Reaction

CDMT00085
stoichiometry: 1.0
species: s45
type: SpeciesReference

re38
kinetic law: CDMT00158
product: CDMT00087
reactant: CDMT00086
fast: false
reversible: false
name: R47: GAP-accelerated GTP-hydrolysis
type: Reaction

CDMT00123
stoichiometry: 1.0
species: s62
type: SpeciesReference

CDMT00090
stoichiometry: 1.0
species: s33
type: SpeciesReference

area
unit: CDMT00013
name: area
type: SBMLUnitsDef

CDMT00071
stoichiometry: 1.0
species: s9
type: SpeciesReference

konBT
constant: true
units: pmps
value: 3.65E7
type: Parameter

re63
kinetic law: CDMT00176
product: CDMT00099
reactant: CDMT00098
fast: false
reversible: false
name: R31: Phosphorylation of Bfa1:Tem1GTP by cytosolic Kin4
type: Reaction

konB5T
constant: true
units: pmps
value: 7000000.0
type: Parameter

re10
kinetic law: CDMT00137
product: CDMT00025
reactant: CDMT00024
reactant: CDMT00023
fast: false
reversible: true
name: R4: Rev. SPB-association of Bfa1-Tem1GTP complex
type: Reaction

s19
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: T-Tem1GTP
type: Species

s1
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 8.33E-5
initial amount: NaN
in compartment: c3
name: B
type: Species

CDMT00038
stoichiometry: 1.0
species: s50
type: SpeciesReference

kfKin4
constant: true
units: ps
value: 1000.0
type: Parameter

CDMT00091
stoichiometry: 1.0
species: s45
type: SpeciesReference

time
unit: CDMT00015
name: time
type: SBMLUnitsDef

CDMT00048
stoichiometry: 1.0
species: s8
type: SpeciesReference

active Bfa1 Cytosol
constant: false
units: molecules
value: 0.0
name: Active Bfa1 in the cytosol
type: Parameter

re50
kinetic law: CDMT00164
product: CDMT00105
reactant: CDMT00104
fast: false
reversible: false
name: R33: Dephosphorylation of Bfa1 by a yet unidentified phosphatase counteracting Kin4
type: Reaction

re23
kinetic law: CDMT00148
product: CDMT00054
reactant: CDMT00053
fast: false
reversible: false
name: R29: Phosphorylation of SPB-bound Bfa1:Tem1GDP by Cdc5
type: Reaction

W3 CDTF: Thu May 10 14:51:07 BST 2012

CDMT00086
stoichiometry: 1.0
species: s29
type: SpeciesReference

CDMT00050
stoichiometry: 1.0
species: s33
type: SpeciesReference

CDMT00122
stoichiometry: 1.0
species: s53
type: SpeciesReference

CDMT00060
stoichiometry: 1.0
species: s37
type: SpeciesReference

CDMT00072
stoichiometry: 1.0
species: s65
type: SpeciesReference

CDMT00092
stoichiometry: 1.0
species: s9
type: SpeciesReference

CDMT00010
stoichiometry: 1.0
species: s1
type: SpeciesReference

CDMT00037
stoichiometry: 1.0
species: s8
type: SpeciesReference

inactive Tem1 SPB
constant: false
units: molecules
value: 0.0
name: Inactive Tem1 at the SPB
type: Parameter

CDMT00020
stoichiometry: 1.0
species: s53
type: SpeciesReference

CDMT00047
stoichiometry: 1.0
species: s25
type: SpeciesReference

http://identifiers.org/biomodels.db/MODEL1202090003#u
constant: false
units: dimensionless
value: 1.0
type: Parameter

CDMT00125
stoichiometry: 1.0
species: s59
type: SpeciesReference

s45
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1P5-Tem1GDP
type: Species

CDMT00105
stoichiometry: 1.0
species: s3
type: SpeciesReference

koffBT
constant: true
units: ps
value: 0.183
type: Parameter

CDMT00008
stoichiometry: 1.0
species: s1
type: SpeciesReference

s59
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1-Tem1GDP
type: Species

kfKin4Cyto
constant: true
units: ps
value: 0.09
type: Parameter

koffT
constant: true
units: ps
value: 0.183
type: Parameter

CDMT00097
stoichiometry: 1.0
species: s19
type: SpeciesReference

koffB
constant: true
units: ps
value: 0.0012
type: Parameter

CDMT00070
stoichiometry: 1.0
species: s7
type: SpeciesReference

CDMT00059
stoichiometry: 1.0
species: s1
type: SpeciesReference

CDMT00104
stoichiometry: 1.0
species: s4
type: SpeciesReference

CDMT00124
stoichiometry: 1.0
species: s50
type: SpeciesReference

spindle alignment
event assignment: CDMT00182
trigger: CDMT00181
use values from trigger time: true
name: SPOC deactivation upon correct spindle alignment
type: Event

CDMT00080
stoichiometry: 1.0
species: s9
type: SpeciesReference

khydBT
constant: true
units: ps
value: 0.00224
type: Parameter

pmps
unit: CDMT00130
unit: CDMT00129
unit: CDMT00128
name: per molar per second
type: SBMLUnitsDef

CDMT00007
stoichiometry: 1.0
species: s4
type: SpeciesReference

NA
constant: true
units: pmole
value: 6.022E23
name: Avogadro's number
type: Parameter

CDMT00039
stoichiometry: 1.0
species: s7
type: SpeciesReference

re7
kinetic law: CDMT00134
product: CDMT00017
reactant: CDMT00016
fast: false
reversible: false
name: R24: Phosphorylation of SPB-bound Bfa1 by Kin4
type: Reaction

CDMT00098
stoichiometry: 1.0
species: s50
type: SpeciesReference

c3
constant: true
units: litre
size: 3.0E-18
spatial dimensions: 3.0
name: SPB
type: Compartment

CDMT00127
stoichiometry: 1.0
species: s65
type: SpeciesReference

CDMT00081
stoichiometry: 1.0
species: s10
type: SpeciesReference

s29
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c3
name: B-Bfa1P4-Tem1GTP
type: Species

re4
kinetic law: CDMT00132
product: CDMT00009
reactant: CDMT00008
reactant: CDMT00007
fast: false
reversible: true
name: R2: Rev. SPB-association of Kin4-phosphorylated Bfa1
type: Reaction

CDMT00095
stoichiometry: 1.0
species: s8
type: SpeciesReference

CDMT00107
stoichiometry: 1.0
species: s3
type: SpeciesReference

CDMT00019
stoichiometry: 1.0
species: s16
type: SpeciesReference

re60
kinetic law: CDMT00174
product: CDMT00074
reactant: CDMT00073
fast: false
reversible: false
name: R34: Dephosphorylation of Bfa1 by a yet unidentified phosphatase counteracting Kin4
type: Reaction

koffB4
constant: true
units: ps
value: 0.0365
type: Parameter

CDMT00106
stoichiometry: 1.0
species: s7
type: SpeciesReference

kfCdc5
constant: true
units: ps
value: 1.0
type: Parameter

CDMT00126
stoichiometry: 1.0
species: s56
type: SpeciesReference

s56
constant: false
boundary condition: false
has only substance units: false
substance units: substance
initial concentration: 0.0
initial amount: NaN
in compartment: c2
name: Bfa1P5-Tem1GTP
type: Species

CDMT00082
stoichiometry: 1.0
species: s37
type: SpeciesReference

CDMT00009
stoichiometry: 1.0
species: s13
type: SpeciesReference

khyd
constant: true
units: ps
value: 0.00224
type: Parameter

re5
kinetic law: CDMT00133
product: CDMT00012
reactant: CDMT00011
reactant: CDMT00010
fast: false
reversible: true
name: R1: Rev. SPB-association of Bfa1
type: Reaction

CDMT00096
stoichiometry: 1.0
species: s2
type: SpeciesReference

re40
kinetic law: CDMT00160
product: CDMT00091
reactant: CDMT00090
fast: false
reversible: false
name: R48: GTP-hydrolysis with intrinisc GTPase activity
type: Reaction

ORG:
  orgname: Deutsches Krebsforschungszentrum Heidelberg
n:
  given: Maiko
  family: Lohel

W3 CDTF: Thu May 10 14:51:07 BST 2012