R2: Rev. SPB-association of Kin4-phosphorylated Bfa1
Deutsches Krebsforschungszentrum Heidelberg
SPOC deactivation upon correct spindle alignment
R21: Rev. association of Tem1GDP with Cdc5-phosphorylated Bfa1
R33: Dephosphorylation of Bfa1 by a yet unidentified phosphatase counteracting Kin4
R4: Rev. SPB-association of Bfa1-Tem1GTP complex
R32: Phosphorylation of Bfa1:Tem1GDP by cytosolic Kin4
This model is from the article: A dynamical model of the spindle position checkpoint Ayse Koca Caydasi, Maiko Lohel, Gerd Grünert, Peter Dittrich, Gislene Pereira & Bashar Ibrahim Molecular Systems Biology 2012; 582 PMEDID , doi: 10.1038/msb.2012.15 Abstract: The orientation of the mitotic spindle with respect to the polarity axis is crucial for the accuracy of asymmetric cell division. In budding yeast, a surveillance mechanism called the spindle position checkpoint (SPOC) prevents exit from mitosis when the mitotic spindle fails to align along the mother-to-daughter polarity axis. SPOC arrest relies upon inhibition of the GTPase Tem1 by the GTPase-activating protein (GAP) complex Bfa1–Bub2. Importantly, reactions signaling mitotic exit take place at yeast centrosomes (named spindle pole bodies, SPBs) and the GAP complex also promotes SPB localization of Tem1. Yet, whether the regulation of Tem1 by Bfa1–Bub2 takes place only at the SPBs remains elusive. Here, we present a quantitative analysis of Bfa1–Bub2 and Tem1 localization at the SPBs. Based on the measured SPB-bound protein levels, we introduce a dynamical model of the SPOC that describes the regulation of Bfa1 and Tem1. Our model suggests that Bfa1 interacts with Tem1 in the cytoplasm as well as at the SPBs to provide efficient Tem1 inhibition. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information. In summary, you are entitled to use this encoded model in absolutely any manner you deem suitable, verbatim, or with modification, alone or embedded it in a larger context, redistribute it, commercially or not, in a restricted way or not. To cite BioModels Database, please use: Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Le Novère N, Laibe C (2010) BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol., 4:92.
R6: Rev. SPB-association of Cdc5-phosphorylated Bfa1-Tem1GTP complex
R36: Dephosphorylation of Cdc5-phosphorylated Bfa1 presumably by PP2A
R25: Phosphorylation of SPB-bound Bfa1:Tem1GTP by Kin4
R19: Rev. association of Tem1GDP with Bfa1
R39: GTP-hydrolysis with intrinisc GTPase activity
R28: Phosphorylation of SPB-bound Bfa1:Tem1GTP by Cdc5
R20: Rev. association of Tem1GDP with Kin4-phosphorylated Bfa1
R17: Rev. association of Tem1GTP with Kin4-phosphorylated Bfa1
R48: GTP-hydrolysis with intrinisc GTPase activity
R15: Rev. association of Tem1GDP with SPB-bound Cdc5-phosphorylated Bfa1
R16: Rev. association of Tem1GTP with Bfa1
R9: Rev. SPB-association of Cdc5-phosphorylated Bfa1-Tem1GDP complex
R5: Rev. SPB-association of Kin4-phosphorylated Bfa1-Tem1GTP complex
R13: Rev. association of Tem1GDP with SPB-bound Bfa1
R31: Phosphorylation of Bfa1:Tem1GTP by cytosolic Kin4
R18: Rev. association of Tem1GTP with Cdc5-phosphorylated Bfa1
R7: Rev. SPB-association of Bfa1-Tem1GDP complex
R41: GTP-hydrolysis with intrinisc GTPase activity
R8: Rev. SPB-association of Kin4 phosphorylated Bfa1-Tem1GDP complex
R38: Dephosphorylation of Cdc5-phosphorylated Bfa1 presumably by PP2A
R45: GTP-hydrolysis with intrinisc GTPase activity
R11: Rev. association of Tem1GTP with SPB-bound Kin4-phosphorylated Bfa1
R29: Phosphorylation of SPB-bound Bfa1:Tem1GDP by Cdc5
R35: Dephosphorylation of Bfa1 by a yet unidentified phosphatase counteracting Kin4
R26: Phosphorylation of SPB-bound Bfa1:Tem1GDP by Kin4
R10: Rev. association of Tem1GTP with SPB-bound Bfa1
R34: Dephosphorylation of Bfa1 by a yet unidentified phosphatase counteracting Kin4
R12: Rev. association of Tem1GTP with SPB-bound Cdc5-phosphorylated Bfa1
R3: Rev. SPB-association of Cdc5-phosphorylated Bfa1
R30: Phosphorylation of Bfa1 by cytosolic Kin4
R14: Rev. association of Tem1GDP with SPB-bound Kin4-phosphorylated Bfa1
R24: Phosphorylation of SPB-bound Bfa1 by Kin4
R37: Dephosphorylation of Cdc5-phosphorylated Bfa1 presumably by PP2A
R27: Phosphorylation of SPB-bound Bfa1 by Cdc5