221119
local parameters: 053416
local parameters: 053414
notes: k1*CaMKII_slash_CaMKII_minus_thr286*PP1_minus_active-k2*PP1_minus_active_slash_Deph_thr286b_slash_Deph_thr286b_cplx
type: KineticLaw

053318
units: 218846
value: 5.0E-12
type: LocalParameter

221530
stoichiometry: 1.0
species: 952782
type: SpeciesReference

221434
stoichiometry: 1.0
species: 952842
type: SpeciesReference

220228
stoichiometry: 1.0
species: 952222
type: SpeciesReference

220414
stoichiometry: 1.0
species: 953122
type: SpeciesReference

220527
stoichiometry: 1.0
species: 952442
type: SpeciesReference

220252
stoichiometry: 1.0
species: 952222
type: SpeciesReference

053328
units: 218882
value: 4.65E-13
type: LocalParameter

219780
stoichiometry: 1.0
species: 952922
type: SpeciesReference

953662
kinetic law: 220635
product: 220623
reactant: 220611
reactant: 220599
fast: false
reversible: true
type: Reaction

220314
stoichiometry: 1.0
species: 953122
type: SpeciesReference

221264
local parameters: 053422
notes: k3*CaM_po_Ca_pc_n_minus_CaNAB_slash_dephosph_neurogranin_slash_dephosph_neurogranin_cplx
type: KineticLaw

053338
units: 218932
value: 3.6E-12
type: LocalParameter

219442
stoichiometry: 2.0
species: 953102
type: SpeciesReference

220466
stoichiometry: 1.0
species: 952322
type: SpeciesReference

219101
exponent: -5.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

220167
stoichiometry: 1.0
species: 952642
type: SpeciesReference

220829
local parameters: 053401
local parameters: 053399
notes: k1*CaMKII_slash_CaMKII_star__star__star_*PP1_minus_active-k2*PP1_minus_active_slash_Deph_minus_thr305_slash_Deph_minus_thr305_cplx
type: KineticLaw

053319
units: 218882
value: 1.0002E-9
type: LocalParameter

053329
units: 218846
value: 1.0E-11
type: LocalParameter

218858
exponent: 1.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

220889
stoichiometry: 1.0
species: 952382
type: SpeciesReference

220913
stoichiometry: 1.0
species: 952542
type: SpeciesReference

220453
stoichiometry: 1.0
species: 952422
type: SpeciesReference

220216
stoichiometry: 1.0
species: 953082
type: SpeciesReference

220264
stoichiometry: 1.0
species: 952802
type: SpeciesReference

220539
local parameters: 053386
local parameters: 053384
notes: k1*CaMKII_slash_CaMKII_minus_thr286*CaMKII_slash_tot_autonomous_CaMKII-k2*CaMKII_slash_tot_autonomous_CaMKII_slash_auton_305_slash_auton_305_cplx
type: KineticLaw

220302
stoichiometry: 1.0
species: 952362
type: SpeciesReference

219611
local parameters: 053331
local parameters: 053330
notes: kf*CaM_slash_neurogranin*CaM_slash_CaM-kb*CaM_slash_neurogranin_minus_CaM
type: KineticLaw

219260
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

221203
stoichiometry: 1.0
species: 952722
type: SpeciesReference

220732
local parameters: 053396
local parameters: 053394
notes: k1*CaMKII_slash_CaMKII_minus_thr286_star__minus_CaM*PP1_minus_active-k2*PP1_minus_active_slash_Deph_minus_thr286_slash_Deph_minus_thr286_cplx
type: KineticLaw

221446
stoichiometry: 1.0
species: 953162
type: SpeciesReference

221107
stoichiometry: 1.0
species: 952582
type: SpeciesReference

219696
local parameters: 053335
local parameters: 053334
notes: kf*PP1_slash_I1_star_*PP1_minus_active-kb*PP1_slash_PP1_minus_I1_star_
type: KineticLaw

053336
units: 218846
value: 1.0E-12
type: LocalParameter

053317
units: 218882
value: 4.99998E-11
type: LocalParameter

219683
stoichiometry: 1.0
species: 952822
type: SpeciesReference

220427
stoichiometry: 1.0
species: 952422
type: SpeciesReference

220877
local parameters: 053402
notes: k3*PP1_minus_active_slash_Deph_minus_thr305_slash_Deph_minus_thr305_cplx
type: KineticLaw

218944
exponent: 3.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

218992
exponent: 4.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

953002
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

220179
stoichiometry: 1.0
species: 953062
type: SpeciesReference

953022
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

219455
stoichiometry: 1.0
species: 952702
type: SpeciesReference

221252
stoichiometry: 1.0
species: 953162
type: SpeciesReference

220925
local parameters: 053406
local parameters: 053404
notes: k1*CaMKII_slash_CaMK_minus_thr306*PP1_minus_active-k2*PP1_minus_active_slash_Deph_minus_thr306_slash_Deph_minus_thr306_cplx
type: KineticLaw

053337
units: 218882
value: 0.0
type: LocalParameter

221059
stoichiometry: 1.0
species: 952482
type: SpeciesReference

053324
units: 218846
value: 7.2E-11
type: LocalParameter

953821
kinetic law: 221362
product: 221350
product: 221338
reactant: 221325
fast: false
reversible: false
type: Reaction

952642
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

952782
constant: false
boundary condition: false
has only substance units: false
initial concentration: 1.8
initial amount: NaN
in compartment: 952162
type: Species

221410
local parameters: 053431
local parameters: 053429
notes: k1*PP1_slash_PP1_minus_I1_star_*CaM_po_Ca_pc_n_minus_CaNAB-k2*CaM_po_Ca_pc_n_minus_CaNAB_slash_dephosph_minus_PP1_minus_I_star__slash_dephosph_minus_PP1_minus_I_star__cplx
type: KineticLaw

220338
local parameters: 053376
local parameters: 053375
notes: k1*CaMKII_slash_CaMKII_minus_thr286*CaMKII_slash_tot_CaM_CaMKII-k2*CaMKII_slash_tot_CaM_CaMKII_slash_CaM_act_305_slash_CaM_act_305_cplx
type: KineticLaw

221215
local parameters: 053421
local parameters: 053419
notes: k1*CaM_slash_neurogranin_star_*CaM_po_Ca_pc_n_minus_CaNAB-k2*CaM_po_Ca_pc_n_minus_CaNAB_slash_dephosph_neurogranin_slash_dephosph_neurogranin_cplx
type: KineticLaw

220937
stoichiometry: 1.0
species: 952542
type: SpeciesReference

219539
stoichiometry: 1.0
species: 953102
type: SpeciesReference

053325
units: 218882
value: 3.6E-12
type: LocalParameter

953622
kinetic law: 220490
product: 220478
product: 220466
reactant: 220453
fast: false
reversible: false
type: Reaction

219467
local parameters: 053324
local parameters: 053323
notes: kf*CaM_slash_CaM*Ca^2-kb*CaM_minus_TR2_minus_Ca2
type: KineticLaw

219138
exponent: 7.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

220792
stoichiometry: 1.0
species: 952342
type: SpeciesReference

219671
stoichiometry: 1.0
species: 952482
type: SpeciesReference

952442
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

218834
exponent: 9.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

953621
kinetic law: 220440
product: 220427
reactant: 220414
reactant: 220401
fast: false
reversible: true
type: Reaction

220277
stoichiometry: 1.0
species: 953082
type: SpeciesReference

220401
stoichiometry: 1.0
species: 952302
type: SpeciesReference

219926
stoichiometry: 1.0
species: 953002
type: SpeciesReference

953762
kinetic law: 221119
product: 221107
reactant: 221095
reactant: 221083
fast: false
reversible: true
type: Reaction

219901
stoichiometry: 1.0
species: 952682
type: SpeciesReference

219113
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

953342
kinetic law: 219563
product: 219551
reactant: 219539
reactant: 219527
notes: Use K3 = 21.5 uM here from Stemmer and Klee table 3. kb/kf =21.5 * 6e5 so kf = 7.75e-7, kb = 10
fast: false
reversible: true
type: Reaction

952182
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

218920
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

220440
local parameters: 053381
local parameters: 053379
notes: k1*CaMKII_slash_CaMKII_minus_CaM*CaMKII_slash_tot_CaM_CaMKII-k2*CaMKII_slash_tot_CaM_CaMKII_slash_CaM_act_286_slash_CaM_act_286_cplx
type: KineticLaw

952702
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

953142
constant: false
boundary condition: true
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

220490
local parameters: 053382
notes: k3*CaMKII_slash_tot_CaM_CaMKII_slash_CaM_act_286_slash_CaM_act_286_cplx
type: KineticLaw

221227
stoichiometry: 1.0
species: 952722
type: SpeciesReference

953961
kinetic law: 221749
product: 221737
product: 221725
reactant: 221712
fast: false
reversible: false
type: Reaction

953162
constant: false
boundary condition: true
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

220326
stoichiometry: 1.0
species: 952402
type: SpeciesReference

953362
kinetic law: 219611
product: 219599
reactant: 219587
reactant: 219575
notes: Surprisingly, no direct info on rates from neurogranin at this time. These rates are based on GAP-43 binding studies. As GAP-43 and neurogranin share near identity in the CaM/PKC binding regions, and also similarity in phosph and dephosph rates, I am borrowing GAP-43 kinetic info. See Alexander et al JBC 262:13 6108-6113 1987
fast: false
reversible: true
type: Reaction

221422
stoichiometry: 1.0
species: 952762
type: SpeciesReference

952162
constant: true
size: 1.0E-12
spatial dimensions: 3.0
type: Compartment

953861
kinetic law: 221506
product: 221494
reactant: 221482
reactant: 221470
notes: PP2A does most of the dephosph of I1 at basal Ca levels. See the review by Cohen in Ann Rev Biochem 1989. For now, lets halve Km. k1 was 3.3e-6, now 6.6e-6
fast: false
reversible: true
type: Reaction

053327
units: 218846
value: 1.0E-11
type: LocalParameter

219479
stoichiometry: 1.0
species: 952702
type: SpeciesReference

953761
kinetic law: 221071
product: 221059
product: 221047
reactant: 221035
fast: false
reversible: false
type: Reaction

220949
stoichiometry: 1.0
species: 952282
type: SpeciesReference

220071
stoichiometry: 1.0
species: 953062
type: SpeciesReference

221591
stoichiometry: 1.0
species: 952902
type: SpeciesReference

219914
stoichiometry: 1.0
species: 952922
type: SpeciesReference

218822
exponent: -9.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

220780
local parameters: 053397
notes: k3*PP1_minus_active_slash_Deph_minus_thr286_slash_Deph_minus_thr286_cplx
type: KineticLaw

219041
exponent: 5.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

952742
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

952842
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

953682
kinetic law: 220732
product: 220719
reactant: 220707
reactant: 220695
notes: The rates are from Stralfors et al Eur J Biochem 149 295-303 giving Vmax = 5.7 umol/min giving k3 = 3.5/sec and k2 = 14. Foulkes et al Eur J Biochem 132 309-313 1983 give Km = 5.1 uM so k1 becomes 5.72e-6 Simonelli 1984 (Grad Thesis, CUNY) showed that other substrates are about 1/10 rate of phosphorylase a, so we reduce k1,k2,k3 by 10 to 5.72e-7, 1.4, 0.35
fast: false
reversible: true
type: Reaction

219053
exponent: -4.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

952902
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

219732
stoichiometry: 1.0
species: 952782
type: SpeciesReference

218870
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

221191
stoichiometry: 1.0
species: 953162
type: SpeciesReference

221567
stoichiometry: 1.0
species: 952822
type: SpeciesReference

953502
kinetic law: 219987
product: 219975
reactant: 219963
reactant: 219950
fast: false
reversible: true
type: Reaction

220599
stoichiometry: 1.0
species: 952302
type: SpeciesReference

219575
stoichiometry: 1.0
species: 952662
type: SpeciesReference

219515
local parameters: 053327
local parameters: 053325
notes: kf*CaM_minus_TR2_minus_Ca2*Ca-kb*CaM_minus_Ca3
type: KineticLaw

219382
local parameters: 053320
local parameters: 053319
notes: kf*CaMKII_slash_CaMKII_minus_thr286*CaM_minus_Ca4-kb*CaMKII_slash_CaMKII_minus_thr286_star__minus_CaM
type: KineticLaw

221239
stoichiometry: 1.0
species: 952662
type: SpeciesReference

220683
local parameters: 053392
notes: k3*CaMKII_slash_tot_autonomous_CaMKII_slash_auton_286_slash_auton_286_cplx
type: KineticLaw

953482
kinetic law: 219889
product: 219877
reactant: 219865
reactant: 219853
notes: Disabled. See notes for PP2B7.g
fast: false
reversible: true
type: Reaction

952882
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

053444
units: 218882
value: 3.42E-14
type: LocalParameter

952862
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.12
initial amount: NaN
in compartment: 952162
type: Species

221035
stoichiometry: 1.0
species: 952562
type: SpeciesReference

221131
stoichiometry: 1.0
species: 952582
type: SpeciesReference

952522
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

221579
stoichiometry: 1.0
species: 952862
type: SpeciesReference

053431
units: 218846
value: 1.36E-12
type: LocalParameter

219065
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

053441
units: 218846
value: 2.5E-11
type: LocalParameter

220131
local parameters: 053370
local parameters: 053369
notes: k1*CaM_slash_neurogranin_minus_CaM*PKC_minus_active-k2*PKC_minus_active_slash_PKC_minus_phosph_minus_ng_minus_CaM_slash_PKC_minus_phosph_minus_ng_minus_CaM_cplx
type: KineticLaw

219744
local parameters: 053337
local parameters: 053336
notes: kf*PP1_slash_PP1_minus_I1-kb*PP1_minus_active*PP1_slash_I1
type: KineticLaw

219889
local parameters: 053344
local parameters: 053343
notes: kf*CaNAB_minus_Ca4*CaM_minus_TR2_minus_Ca2-kb*PP2B_slash_CaMCa2_minus_CANAB
type: KineticLaw

219587
stoichiometry: 1.0
species: 952602
type: SpeciesReference

219527
stoichiometry: 1.0
species: 952682
type: SpeciesReference

220587
local parameters: 053387
notes: k3*CaMKII_slash_tot_autonomous_CaMKII_slash_auton_305_slash_auton_305_cplx
type: KineticLaw

952502
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

220671
stoichiometry: 1.0
species: 953142
type: SpeciesReference

953681
kinetic law: 220683
product: 220671
product: 220659
reactant: 220647
fast: false
reversible: false
type: Reaction

053432
units: 218846
value: 3.4E-13
type: LocalParameter

053442
units: 218846
value: 6.0E-12
type: LocalParameter

219394
stoichiometry: 1.0
species: 952282
type: SpeciesReference

953501
kinetic law: 219938
product: 219926
reactant: 219914
reactant: 219901
fast: false
reversible: true
type: Reaction

218639
exponent: -4.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

221047
stoichiometry: 1.0
species: 952382
type: SpeciesReference

053447
units: 218846
value: 3.4E-14
type: LocalParameter

220575
stoichiometry: 1.0
species: 953142
type: SpeciesReference

953801
kinetic law: 221264
product: 221252
product: 221239
reactant: 221227
fast: false
reversible: false
type: Reaction

952582
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

219599
stoichiometry: 1.0
species: 952622
type: SpeciesReference

220805
stoichiometry: 1.0
species: 952482
type: SpeciesReference

952222
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

218688
exponent: 5.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

220143
stoichiometry: 1.0
species: 952202
type: SpeciesReference

952202
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

220514
stoichiometry: 1.0
species: 953142
type: SpeciesReference

218894
exponent: 2.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

218956
exponent: -2.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

953602
kinetic law: 220388
product: 220375
product: 220362
reactant: 220350
fast: false
reversible: false
type: Reaction

221543
stoichiometry: 1.0
species: 952862
type: SpeciesReference

218603
exponent: -3.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

952242
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

221011
stoichiometry: 1.0
species: 952562
type: SpeciesReference

953802
kinetic law: 221313
product: 221301
reactant: 221289
reactant: 221276
fast: false
reversible: true
type: Reaction

219406
stoichiometry: 1.0
species: 952362
type: SpeciesReference

953262
kinetic law: 219382
product: 219370
reactant: 219358
reactant: 219346
notes: Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3
fast: false
reversible: true
type: Reaction

952282
constant: false
boundary condition: false
has only substance units: false
initial concentration: 70.0
initial amount: NaN
in compartment: 952162
type: Species

219503
stoichiometry: 1.0
species: 952682
type: SpeciesReference

952262
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

953282
kinetic law: 219418
product: 219406
reactant: 219394
notes: This reaction represents one of the big unknowns in CaMK-II biochemistry: what maintains the basal level of phosphorylation on thr 286 ? See Hanson and Schulman Ann Rev Biochem 1992 61:559-601, specially pg 580, for review. I have not been able to find any compelling mechanism in the literature, but fortunately the level of basal activity is well documented.
fast: false
reversible: true
type: Reaction

953601
kinetic law: 220338
product: 220326
reactant: 220314
reactant: 220302
notes: Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. Jan 1 1998: Speed up 12x to match fig 5.
fast: false
reversible: true
type: Reaction

218530
exponent: -1.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

220817
stoichiometry: 1.0
species: 952522
type: SpeciesReference

953521
kinetic law: 220035
product: 220023
reactant: 220011
reactant: 219999
notes: Rates from Huang et al ABB 305:2 570-580 1993
fast: false
reversible: true
type: Reaction

953582
kinetic law: 220290
product: 220277
product: 220264
reactant: 220252
fast: false
reversible: false
type: Reaction

220563
stoichiometry: 1.0
species: 952342
type: SpeciesReference

219708
stoichiometry: 1.0
species: 952842
type: SpeciesReference

220203
stoichiometry: 1.0
species: 952782
type: SpeciesReference

953562
kinetic law: 220191
product: 220179
product: 220167
product: 220155
reactant: 220143
fast: false
reversible: false
type: Reaction

218968
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

219089
exponent: 6.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

221555
local parameters: 053437
notes: k3*PP2A_slash_PP2A_minus_dephosph_minus_I1_slash_PP2A_minus_dephosph_minus_I1_cplx
type: KineticLaw

220155
stoichiometry: 1.0
species: 952602
type: SpeciesReference

219720
stoichiometry: 1.0
species: 952482
type: SpeciesReference

953881
kinetic law: 221555
product: 221543
product: 221530
reactant: 221518
fast: false
reversible: false
type: Reaction

220502
stoichiometry: 1.0
species: 952362
type: SpeciesReference

219418
local parameters: 053322
local parameters: 053321
notes: kf*CaMKII_slash_CaMKII-kb*CaMKII_slash_CaMKII_minus_thr286
type: KineticLaw

220478
stoichiometry: 1.0
species: 953122
type: SpeciesReference

053446
units: 218846
value: 1.36E-13
type: LocalParameter

953522
kinetic law: 220083
product: 220071
product: 220059
reactant: 220047
fast: false
reversible: false
type: Reaction

953581
kinetic law: 220240
product: 220228
reactant: 220216
reactant: 220203
notes: #s from Bramson et al CRC crit rev Biochem 15:2 93-124. They have a huge list of peptide substrates and I have chosen high-ish rates. These consts give too much PKA activity, so lower Vmax 1/3. Now, k1 = 3e-5, k2 = 36, k3 = 9 (still pretty fast). Also lower Km 1/3 so k1 = 1e-5 Cohen et al FEBS Lett 76:182-86 1977 say rate =30% PKA act on phosphokinase beta.
fast: false
reversible: true
type: Reaction

218615
exponent: 3.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

221023
local parameters: 053411
local parameters: 053409
notes: k1*CaMKII_slash_CaMKII_star__star__star_*PP1_minus_active-k2*PP1_minus_active_slash_Deph_minus_thr286c_slash_Deph_minus_thr286c_cplx
type: KineticLaw

952722
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

219792
local parameters: 053340
local parameters: 053338
notes: kf*Ca^2*PP2B_slash_CaNAB_minus_Ca2-kb*CaNAB_minus_Ca4
type: KineticLaw

952822
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

218542
exponent: 1.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

219370
stoichiometry: 1.0
species: 952322
type: SpeciesReference

953542
kinetic law: 220131
product: 220119
reactant: 220107
reactant: 220095
notes: Rates are 60% those of PKC-phosph-neurogranin. See Huang et al ABB 305:2 570-580 1993
fast: false
reversible: true
type: Reaction

220240
local parameters: 053373
local parameters: 053372
notes: k1*PP1_slash_I1*PKA_minus_active-k2*PKA_minus_active_slash_PKA_minus_phosph_minus_I1_slash_PKA_minus_phosph_minus_I1_cplx
type: KineticLaw

953721
kinetic law: 220877
product: 220865
product: 220853
reactant: 220841
fast: false
reversible: false
type: Reaction

219768
stoichiometry: 1.0
species: 952982
type: SpeciesReference

219358
stoichiometry: 1.0
species: 952262
type: SpeciesReference

953102
constant: true
boundary condition: true
has only substance units: false
initial concentration: 0.08
initial amount: NaN
in compartment: 952162
type: Species

219805
stoichiometry: 1.0
species: 952962
type: SpeciesReference

053412
units: 218846
value: 3.5E-13
type: LocalParameter

953701
kinetic law: 220780
product: 220768
product: 220756
reactant: 220744
fast: false
reversible: false
type: Reaction

053426
units: 218846
value: 1.36E-12
type: LocalParameter

219199
exponent: -7.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

220986
stoichiometry: 1.0
species: 952342
type: SpeciesReference

219963
stoichiometry: 1.0
species: 952922
type: SpeciesReference

219285
exponent: 1.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

219865
stoichiometry: 1.0
species: 952702
type: SpeciesReference

053411
units: 218846
value: 1.4E-12
type: LocalParameter

221603
local parameters: 053441
local parameters: 053439
notes: k1*PP1_slash_PP1_minus_I1_star_*PP2A-k2*PP2A_slash_PP2A_minus_dephosph_minus_PP1_minus_I_star__slash_PP2A_minus_dephosph_minus_PP1_minus_I_star__cplx
type: KineticLaw

221167
local parameters: 053417
notes: k3*PP1_minus_active_slash_Deph_thr286b_slash_Deph_thr286b_cplx
type: KineticLaw

053439
units: 218882
value: 3.96E-12
type: LocalParameter

219950
stoichiometry: 1.0
species: 952262
type: SpeciesReference

221518
stoichiometry: 1.0
species: 952882
type: SpeciesReference

220999
stoichiometry: 1.0
species: 952482
type: SpeciesReference

218652
exponent: 4.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

218579
exponent: 2.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

220623
stoichiometry: 1.0
species: 952462
type: SpeciesReference

MODEL9080747936
reaction: 953801
units def: 219175
units def: 218738
species: 952242
species: 952482
species: 953082
species: 952902
units def: 218932
units def: 218701
reaction: 953581
reaction: 953422
type: SBMLModel
reaction: 953662
species: 952182
species: 952642
reaction: 953721
species: 952462
units def: 219029
species: 953062
species: 953122
reaction: 953821
reaction: 953782
species: 952342
reaction: 953762
units def: 218518
species: 952522
species: 952922
reaction: 953442
units def: 218554
reaction: 953262
units def: 218980
species: 952422
reaction: 953501
species: 952542
species: 952782
reaction: 953402
reaction: 953742
reaction: 953562
units def: 218591
reaction: 953861
species: 953142
species: 952962
units def: 218627
species: 952302
reaction: 953242
species: 952862
species: 953022
reaction: 953482
reaction: 953521
compartment: 952162
units def: 218882
species: 952582
species: 952262
species: 952942
species: 952282
reaction: 953841
reaction: 953582
reaction: 953382
units def: 218810
reaction: 953921
reaction: 953702
reaction: 953641
species: 952742
curated: false
units def: 218846
species: 952202
species: 953042
name: Bhalla2004_CaMKII_2003
species: 953102
species: 952362
species: 952722
units def: 218664
reaction: 953642
reaction: 953621
reaction: 953941
reaction: 953681
reaction: 953722
species: 952682
time units: 131570
units def: 219273

219817
stoichiometry: 2.0
species: 953102
type: SpeciesReference

952482
constant: false
boundary condition: false
has only substance units: false
initial concentration: 1.8
initial amount: NaN
in compartment: 952162
type: Species

053368
units: 218846
value: 5.8E-13
type: LocalParameter

952402
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

953462
kinetic law: 219841
product: 219829
reactant: 219817
reactant: 219805
notes: going on the experience with CaM, we put the fast (high affinity) sites first. We only know (Stemmer and Klee) that the affinity is < 70 nM. Assuming 10 nM at first, we get kf = 2.78e-8, kb = 1. Try 20 nM. kf = 7e-9, kb = 1
fast: false
reversible: true
type: Reaction

219877
stoichiometry: 1.0
species: 953022
type: SpeciesReference

952302
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

219187
exponent: 8.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

953422
kinetic law: 219744
product: 219732
product: 219720
reactant: 219708
notes: Let us assume that the equil in this case is very far over to the right. This is probably safe.
fast: false
reversible: true
type: Reaction

053389
units: 218882
value: 1.4286E-14
type: LocalParameter

220350
stoichiometry: 1.0
species: 952402
type: SpeciesReference

221179
stoichiometry: 1.0
species: 952642
type: SpeciesReference

952922
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

219309
stoichiometry: 1.0
species: 952282
type: SpeciesReference

219756
stoichiometry: 2.0
species: 953102
type: SpeciesReference

053369
units: 218882
value: 6.12E-14
type: LocalParameter

053437
units: 218846
value: 6.0E-12
type: LocalParameter

220551
stoichiometry: 1.0
species: 952442
type: SpeciesReference

053427
units: 218846
value: 3.4E-13
type: LocalParameter

221458
local parameters: 053432
notes: k3*CaM_po_Ca_pc_n_minus_CaNAB_slash_dephosph_minus_PP1_minus_I_star__slash_dephosph_minus_PP1_minus_I_star__cplx
type: KineticLaw

220611
stoichiometry: 1.0
species: 953142
type: SpeciesReference

221506
local parameters: 053436
local parameters: 053434
notes: k1*PP1_slash_I1_star_*PP2A-k2*PP2A_slash_PP2A_minus_dephosph_minus_I1_slash_PP2A_minus_dephosph_minus_I1_cplx
type: KineticLaw

952602
constant: false
boundary condition: false
has only substance units: false
initial concentration: 20.0
initial amount: NaN
in compartment: 952162
type: Species

053416
units: 218846
value: 1.4E-12
type: LocalParameter

220962
stoichiometry: 1.0
species: 952482
type: SpeciesReference

220023
stoichiometry: 1.0
species: 952182
type: SpeciesReference

218566
exponent: -2.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

953402
kinetic law: 219696
product: 219683
reactant: 219671
reactant: 219659
notes: K inhib = 1nM from Cohen Ann Rev Bioch 1989, 4 nM from Foukes et al Assume 2 nM. kf /kb = 8.333e-4
fast: false
reversible: true
type: Reaction

053402
units: 218846
value: 3.5E-13
type: LocalParameter

219841
local parameters: 053342
local parameters: 053341
notes: kf*PP2B_slash_CaNAB*Ca^2-kb*PP2B_slash_CaNAB_minus_Ca2
type: KineticLaw

952382
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

220695
stoichiometry: 1.0
species: 952322
type: SpeciesReference

053366
units: 218882
value: 1.02E-13
type: LocalParameter

952362
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

221712
stoichiometry: 1.0
species: 952942
type: SpeciesReference

952682
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

053422
units: 218846
value: 6.7E-13
type: LocalParameter

053376
units: 218846
value: 2.4E-11
type: LocalParameter

053436
units: 218846
value: 2.5E-11
type: LocalParameter

219829
stoichiometry: 1.0
species: 952982
type: SpeciesReference

218762
exponent: 7.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

952322
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

952942
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

953322
kinetic law: 219515
product: 219503
reactant: 219491
reactant: 219479
notes: K3 = 21.5, K4 = 2.8. Assuming that the K4 step happens first, we get kb/kf = 2.8 uM = 1.68e6 so kf =6e-6 assuming kb = 10
fast: false
reversible: true
type: Reaction

053401
units: 218846
value: 1.4E-12
type: LocalParameter

219563
local parameters: 053329
local parameters: 053328
notes: kf*CaM_minus_Ca3*Ca-kb*CaM_minus_Ca4
type: KineticLaw

952982
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

221688
stoichiometry: 1.0
species: 952942
type: SpeciesReference

219491
stoichiometry: 1.0
species: 953102
type: SpeciesReference

218676
exponent: -5.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

220647
stoichiometry: 1.0
species: 952462
type: SpeciesReference

053367
units: 218846
value: 2.34E-12
type: LocalParameter

953302
kinetic law: 219467
product: 219455
reactant: 219442
reactant: 219430
notes: Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !)....
fast: false
reversible: true
type: Reaction

952342
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

221143
stoichiometry: 1.0
species: 952282
type: SpeciesReference

053377
units: 218846
value: 6.0E-12
type: LocalParameter

053421
units: 218846
value: 2.67E-12
type: LocalParameter

221627
stoichiometry: 1.0
species: 952842
type: SpeciesReference

053414
units: 218882
value: 3.432E-13
type: LocalParameter

219853
stoichiometry: 1.0
species: 952922
type: SpeciesReference

219297
stoichiometry: 1.0
species: 952262
type: SpeciesReference

053404
units: 218882
value: 3.432E-13
type: LocalParameter

220974
local parameters: 053407
notes: k3*PP1_minus_active_slash_Deph_minus_thr306_slash_Deph_minus_thr306_cplx
type: KineticLaw

220011
stoichiometry: 1.0
species: 953062
type: SpeciesReference

219212
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

221700
local parameters: 053446
local parameters: 053444
notes: k1*PP1_slash_I1_star_*CaNAB_minus_Ca4-k2*CaNAB_minus_Ca4_slash_dephosph_inhib1_noCaM_slash_dephosph_inhib1_noCaM_cplx
type: KineticLaw

953921
kinetic law: 221652
product: 221640
product: 221627
reactant: 221615
fast: false
reversible: false
type: Reaction

053364
units: 218846
value: 1.0E-12
type: LocalParameter

053424
units: 218882
value: 3.42E-13
type: LocalParameter

053434
units: 218882
value: 3.96E-12
type: LocalParameter

219551
stoichiometry: 1.0
species: 952262
type: SpeciesReference

221676
stoichiometry: 1.0
species: 952922
type: SpeciesReference

221350
stoichiometry: 1.0
species: 953162
type: SpeciesReference

220635
local parameters: 053391
local parameters: 053389
notes: k1*CaMKII_slash_CaMKII_minus_CaM*CaMKII_slash_tot_autonomous_CaMKII-k2*CaMKII_slash_tot_autonomous_CaMKII_slash_auton_286_slash_auton_286_cplx
type: KineticLaw

220375
stoichiometry: 1.0
species: 953122
type: SpeciesReference

218750
exponent: -7.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

220388
local parameters: 053377
notes: k3*CaMKII_slash_tot_CaM_CaMKII_slash_CaM_act_305_slash_CaM_act_305_cplx
type: KineticLaw

953722
kinetic law: 220925
product: 220913
reactant: 220901
reactant: 220889
notes: See Cohen et al
fast: false
reversible: true
type: Reaction

221155
stoichiometry: 1.0
species: 952482
type: SpeciesReference

221615
stoichiometry: 1.0
species: 952902
type: SpeciesReference

053379
units: 218882
value: 2.20068E-14
type: LocalParameter

221494
stoichiometry: 1.0
species: 952882
type: SpeciesReference

053343
units: 218882
value: 2.4E-13
type: LocalParameter

221482
stoichiometry: 1.0
species: 952862
type: SpeciesReference

053372
units: 218882
value: 6.0E-12
type: LocalParameter

053391
units: 218846
value: 2.0E-12
type: LocalParameter

952802
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.001
initial amount: NaN
in compartment: 952162
type: Species

219938
local parameters: 053346
local parameters: 053345
notes: kf*CaM_minus_Ca3*CaNAB_minus_Ca4-kb*PP2B_slash_CaMCa3_minus_CaNAB
type: KineticLaw

953042
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

219999
stoichiometry: 1.0
species: 952662
type: SpeciesReference

221725
stoichiometry: 1.0
species: 952782
type: SpeciesReference

053381
units: 218846
value: 2.0E-12
type: LocalParameter

221095
stoichiometry: 1.0
species: 952482
type: SpeciesReference

953062
constant: true
boundary condition: true
has only substance units: false
initial concentration: 0.09
initial amount: NaN
in compartment: 952162
type: Species

053406
units: 218846
value: 1.4E-12
type: LocalParameter

053333
units: 218846
value: 0.0
type: LocalParameter

053382
units: 218846
value: 5.0E-13
type: LocalParameter

221338
stoichiometry: 1.0
species: 952782
type: SpeciesReference

218786
exponent: -8.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

220095
stoichiometry: 1.0
species: 952622
type: SpeciesReference

221664
stoichiometry: 1.0
species: 952802
type: SpeciesReference

053323
units: 218932
value: 7.2E-11
type: LocalParameter

221362
local parameters: 053427
notes: k3*CaM_po_Ca_pc_n_minus_CaNAB_slash_dephosph_inhib1_slash_dephosph_inhib1_cplx
type: KineticLaw

220853
stoichiometry: 1.0
species: 952362
type: SpeciesReference

953781
kinetic law: 221167
product: 221155
product: 221143
reactant: 221131
fast: false
reversible: false
type: Reaction

221301
stoichiometry: 1.0
species: 952742
type: SpeciesReference

053342
units: 218846
value: 1.0E-12
type: LocalParameter

053373
units: 218846
value: 3.6E-11
type: LocalParameter

953382
kinetic law: 219647
product: 219635
reactant: 219623
notes: This is put in to keep the basal levels of neurogranin* experimentally reasonable. From various papers, specially Ramakers et al JBC 270:23 1995 13892-13898, it looks like the basal level of phosph is between 20 and 40%. I est around 25 % The kf of 0.005 gives around this level at basal PKC activity levels of 0.1 uM active PKC.
fast: false
reversible: true
type: Reaction

053392
units: 218846
value: 5.0E-13
type: LocalParameter

219635
stoichiometry: 1.0
species: 952662
type: SpeciesReference

220047
stoichiometry: 1.0
species: 952182
type: SpeciesReference

220191
local parameters: 053371
notes: k3*PKC_minus_active_slash_PKC_minus_phosph_minus_ng_minus_CaM_slash_PKC_minus_phosph_minus_ng_minus_CaM_cplx
type: KineticLaw

053332
units: 218846
value: 5.0E-15
type: LocalParameter

220756
stoichiometry: 1.0
species: 952302
type: SpeciesReference

219151
exponent: -6.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

221398
stoichiometry: 1.0
species: 952762
type: SpeciesReference

053419
units: 218882
value: 3.336E-13
type: LocalParameter

953082
constant: true
boundary condition: true
has only substance units: false
initial concentration: 0.015
initial amount: NaN
in compartment: 952162
type: Species

953122
constant: false
boundary condition: true
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

053322
units: 218846
value: 0.0
type: LocalParameter

953782
kinetic law: 221215
product: 221203
reactant: 221191
reactant: 221179
notes: From Seki et al ABB 316(2):673-679
fast: false
reversible: true
type: Reaction

053341
units: 218932
value: 1.0008E-8
type: LocalParameter

221737
stoichiometry: 1.0
species: 952922
type: SpeciesReference

053374
units: 218846
value: 9.0E-12
type: LocalParameter

221470
stoichiometry: 1.0
species: 952802
type: SpeciesReference

220707
stoichiometry: 1.0
species: 952482
type: SpeciesReference

219321
stoichiometry: 1.0
species: 952302
type: SpeciesReference

952462
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

220083
local parameters: 053368
notes: k3*PKC_minus_active_slash_PKC_minus_phosph_minus_neurogranin_slash_PKC_minus_phosph_minus_neurogranin_cplx
type: KineticLaw

218726
exponent: 6.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

221313
local parameters: 053426
local parameters: 053424
notes: k1*PP1_slash_I1_star_*CaM_po_Ca_pc_n_minus_CaNAB-k2*CaM_po_Ca_pc_n_minus_CaNAB_slash_dephosph_inhib1_slash_dephosph_inhib1_cplx
type: KineticLaw

220659
stoichiometry: 1.0
species: 952322
type: SpeciesReference

221652
local parameters: 053442
notes: k3*PP2A_slash_PP2A_minus_dephosph_minus_PP1_minus_I_star__slash_PP2A_minus_dephosph_minus_PP1_minus_I_star__cplx
type: KineticLaw

220865
stoichiometry: 1.0
species: 952482
type: SpeciesReference

953442
kinetic law: 219792
product: 219780
reactant: 219768
reactant: 219756
notes: This process is probably much more complicated and involves CaM. However, as I can\'t find detailed info I am bundling this into a single step. Based on Steemer and Klee pg 6863, the Kact is 0.5 uM. kf/kb = 1/(0.5 * 6e5)^2 = 1.11e-11
fast: false
reversible: true
type: Reaction

053321
units: 218846
value: 3.0E-15
type: LocalParameter

053394
units: 218882
value: 3.432E-13
type: LocalParameter

220362
stoichiometry: 1.0
species: 952342
type: SpeciesReference

221083
stoichiometry: 1.0
species: 952362
type: SpeciesReference

053335
units: 218846
value: 1.0E-13
type: LocalParameter

053375
units: 218882
value: 2.64084E-13
type: LocalParameter

053340
units: 218846
value: 1.0E-12
type: LocalParameter

219236
exponent: 9.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

952562
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

218907
exponent: -1.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

053407
units: 218846
value: 3.5E-13
type: LocalParameter

053334
units: 218882
value: 4.9998E-10
type: LocalParameter

221325
stoichiometry: 1.0
species: 952742
type: SpeciesReference

952542
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

219623
stoichiometry: 1.0
species: 952642
type: SpeciesReference

218713
exponent: -6.0
scale: 0
multipler: 1.0
kind: LITRE
type: SBMLUnit

220744
stoichiometry: 1.0
species: 952502
type: SpeciesReference

220035
local parameters: 053367
local parameters: 053366
notes: k1*CaM_slash_neurogranin*PKC_minus_active-k2*PKC_minus_active_slash_PKC_minus_phosph_minus_neurogranin_slash_PKC_minus_phosph_minus_neurogranin_cplx
type: KineticLaw

221386
stoichiometry: 1.0
species: 953162
type: SpeciesReference

053320
units: 218846
value: 1.0E-13
type: LocalParameter

053417
units: 218846
value: 3.5E-13
type: LocalParameter

952145
constant: true
size: 1.0E-18
spatial dimensions: 3.0
type: Compartment

221071
local parameters: 053412
notes: k3*PP1_minus_active_slash_Deph_minus_thr286c_slash_Deph_minus_thr286c_cplx
type: KineticLaw

053347
units: 218882
value: 6.0E-10
type: LocalParameter

952622
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

220901
stoichiometry: 1.0
species: 952482
type: SpeciesReference

221640
stoichiometry: 1.0
species: 952862
type: SpeciesReference

220719
stoichiometry: 1.0
species: 952502
type: SpeciesReference

053396
units: 218846
value: 1.4E-12
type: LocalParameter

953641
kinetic law: 220539
product: 220527
reactant: 220514
reactant: 220502
notes: See Hanson and Schulman again, for afterburst rates of phosph.
fast: false
reversible: true
type: Reaction

220290
local parameters: 053374
notes: k3*PKA_minus_active_slash_PKA_minus_phosph_minus_I1_slash_PKA_minus_phosph_minus_I1_cplx
type: KineticLaw

952422
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

053386
units: 218846
value: 2.4E-11
type: LocalParameter

219659
stoichiometry: 1.0
species: 952802
type: SpeciesReference

221749
local parameters: 053447
notes: k3*CaNAB_minus_Ca4_slash_dephosph_inhib1_noCaM_slash_dephosph_inhib1_noCaM_cplx
type: KineticLaw

219430
stoichiometry: 1.0
species: 952602
type: SpeciesReference

053409
units: 218882
value: 3.432E-13
type: LocalParameter

053429
units: 218882
value: 3.42E-13
type: LocalParameter

053346
units: 218846
value: 1.0E-12
type: LocalParameter

221374
stoichiometry: 1.0
species: 952822
type: SpeciesReference

952662
constant: false
boundary condition: false
has only substance units: false
initial concentration: 10.0
initial amount: NaN
in compartment: 952162
type: Species

219987
local parameters: 053364
local parameters: 053347
notes: kf*CaM_minus_Ca4*CaNAB_minus_Ca4-kb*PP2B_slash_CaMCa4_minus_CaNAB
type: KineticLaw

952962
constant: false
boundary condition: false
has only substance units: false
initial concentration: 1.0
initial amount: NaN
in compartment: 952162
type: Species

220107
stoichiometry: 1.0
species: 953062
type: SpeciesReference

219004
exponent: -3.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

219334
local parameters: 053318
local parameters: 053317
notes: kf*CaM_minus_Ca4*CaMKII_slash_CaMKII-kb*CaMKII_slash_CaMKII_minus_CaM
type: KineticLaw

221276
stoichiometry: 1.0
species: 952802
type: SpeciesReference

053397
units: 218846
value: 3.5E-13
type: LocalParameter

053387
units: 218846
value: 6.0E-12
type: LocalParameter

952762
constant: false
boundary condition: false
has only substance units: false
initial concentration: 0.0
initial amount: NaN
in compartment: 952162
type: Species

219248
exponent: -8.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

220841
stoichiometry: 1.0
species: 952522
type: SpeciesReference

953741
kinetic law: 220974
product: 220962
product: 220949
reactant: 220937
fast: false
reversible: false
type: Reaction

218798
exponent: 8.0
scale: -6
multipler: 1.0
kind: MOLE
type: SBMLUnit

053345
units: 218882
value: 2.238E-12
type: LocalParameter

953841
kinetic law: 221410
product: 221398
reactant: 221386
reactant: 221374
fast: false
reversible: true
type: Reaction

953842
kinetic law: 221458
product: 221446
product: 221434
reactant: 221422
fast: false
reversible: false
type: Reaction

219647
local parameters: 053333
local parameters: 053332
notes: kf*CaM_slash_neurogranin_star_-kb*CaM_slash_neurogranin
type: KineticLaw

053384
units: 218882
value: 1.71426E-13
type: LocalParameter

053331
units: 218846
value: 1.0E-12
type: LocalParameter

953941
kinetic law: 221700
product: 221688
reactant: 221676
reactant: 221664
notes: The rates here are so slow I do not know if we should even bother with this enz reacn. These numbers are from Liu and Storm. Other refs suggest that the Km stays the same but the Vmax goes to 10% of the CaM stim levels. Prev: k1=2.2e-9, k2 = 0.0052, k3 = 0.0013 New : k1=5.7e-8, k2=.136, k3=.034
fast: false
reversible: true
type: Reaction

219017
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

221289
stoichiometry: 1.0
species: 953162
type: SpeciesReference

219975
stoichiometry: 1.0
species: 953042
type: SpeciesReference

953702
kinetic law: 220829
product: 220817
reactant: 220805
reactant: 220792
fast: false
reversible: true
type: Reaction

220119
stoichiometry: 1.0
species: 952202
type: SpeciesReference

953742
kinetic law: 221023
product: 221011
reactant: 220999
reactant: 220986
fast: false
reversible: true
type: Reaction

953242
kinetic law: 219334
product: 219321
reactant: 219309
reactant: 219297
notes: This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kb/Kf = 6e4 #/cell. Rate is fast (see Hanson et al Neuron 12 943-956 1994) so lets say kb = 10. This gives kf = 1.6667e-4 H&S AnnRev Biochem 92 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol.
fast: false
reversible: true
type: Reaction

220059
stoichiometry: 1.0
species: 952642
type: SpeciesReference

953901
kinetic law: 221603
product: 221591
reactant: 221579
reactant: 221567
notes: k1 changed from 3.3e-6 to 6.6e-6
fast: false
reversible: true
type: Reaction

053371
units: 218846
value: 3.5E-13
type: LocalParameter

219163
exponent: -1.0
scale: 0
multipler: 1.0
kind: SECOND
type: SBMLUnit

220768
stoichiometry: 1.0
species: 952482
type: SpeciesReference

053344
units: 218846
value: 1.0E-12
type: LocalParameter

953642
kinetic law: 220587
product: 220575
product: 220563
reactant: 220551
fast: false
reversible: false
type: Reaction

053399
units: 218882
value: 3.432E-13
type: LocalParameter

053370
units: 218846
value: 1.4E-12
type: LocalParameter

219346
stoichiometry: 1.0
species: 952362
type: SpeciesReference

053330
units: 218882
value: 3.0E-13
type: LocalParameter