Itgax UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.0679254531571
p value: 0.0185529006464801
refers to: Itgax
refers to: 1380077_at
t statistic: 4.32731970928082

(Sample) disease/diet-induced obesity
type: EFO_0001073
property type: disease
property value: diet-induced obesity

E-GEOD-8700
type: microarray 1colour mrna differential
RO_0002162: Rattus norvegicus
description: Analysis of gene expression profiles of epididymal fat from DIO rats; We applied a comparative functional genomics approach to evaluate diet-induced obese (DIO) rats as an obesity model Experiment Overall Design: Gene expression profiles from DIO and lean rats were generated and compared. A private colony of male Long-Evans rats was set up at Harlan (Harlan-Sprague Dawley, Indianapolis, IN). The animals had been on either a high fat/high sucrose diet (TD95217, Harlan Takled, Madison, WI) or a regular chow (Purina 5008; Ralston-Purina, St. Louis, MO) since weaning. Rats were shipped at 14-weeks of age to our facility and maintained on a 12:12-hour light-dark photoperiod (light on 10:00 PM). They were individually housed throughout the study at ambient temperature, and had free access to diets and water. After 2 weeks acclimation to the facility, body weight of the rats and weight of food consumed in the last 7 days were measured once a week. Body fat mass was analyzed by nuclear magnetic resonance (NMR) using an Echo Medical System (Houston, TX) instrument 3 times throughout the study. Fat free mass is the difference between body mass and fat mass. All animal studies were conducted in compliance with approved institutional animal care and use protocols according to NIH guidelines (NIH publication No. 86-23, 1985). OGTT was conducted after an overnight fasting when animals were 21 weeks of age. After a baseline blood collection (time 0), animals were dosed orally with 1.5 gram/kg glucose. Subsequence blood samples were collected at 30, 60, and 120 minutes post glucose challenge for the determination of plasma glucose and insulin levels. At 25 weeks of age, blood was collected by tail bleeding after overnight fasting. Rats were killed a week later at 09:00am by rapid decapitation. Trunk blood was collected and epididymal fat tissue excised. Various circulating metabolic parameters were measured under fasted (week 25) and fed conditions (week 26). Total RNA from rat epi…
uses data from: http://identifiers.org/pubmed/18239588

Fabp5 UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.11448122874917
p value: 0.00869214075210637
refers to: Fabp5
refers to: 1370281_at
t statistic: 6.43141775523011

Spp1
type: EnsemblDatabaseReference

(Factor value) DISEASE/diet-induced obesity
type: EFO_0001073
property type: DISEASE
property value: diet-induced obesity

(Assay) control lean rat A5
type: Assay
identifier: control lean rat A5
has participant: A-AFFY-43

Ccl9
type: EnsemblDatabaseReference

(Sample) age/16 to 26
type: EFO_0000001
property type: age
property value: 16 to 26

(Assay) control lean rat A2
type: Assay
identifier: control lean rat A2
has participant: A-AFFY-43

(Sample) disease/lean control
type: EFO_0000001
property type: disease
property value: lean control

(Sample) organism part/epididymal fat
type: EFO_0000001
property type: organism part
property value: epididymal fat

Dnase1l3
type: EnsemblDatabaseReference

(Assay) control lean rat A7
type: Assay
identifier: control lean rat A7
has participant: A-AFFY-43

Scd1
type: EnsemblDatabaseReference

(Assay) Diet Induced Obese rat C6
type: Assay
identifier: Diet Induced Obese rat C6
has participant: A-AFFY-43

Fabp5
type: EnsemblDatabaseReference

1387189_at
type: EFO_0005072
part of: A-AFFY-43

Ccl9 UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.34537497689992
p value: 0.0140093449519682
refers to: Ccl9
refers to: 1392172_at
t statistic: 4.83030970378562

Plin2 UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.04371426184298
p value: 0.0111043418782167
refers to: Plin2
refers to: 1390383_at
t statistic: 5.68424036268578

Fabp5
type: EnsemblDatabaseReference

Krt79 UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.09582139976743
p value: 0.0117055209526133
refers to: Krt79
refers to: 1377246_at
t statistic: 5.34206127011608

Cd5l UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 2.0289249109832
p value: 0.02126813148102
refers to: 1385635_at
refers to: Cd5l
t statistic: 4.06739945907386

Atp1b1
type: EnsemblDatabaseReference

A-AFFY-43
type: EFO_0000269

1368187_at
type: EFO_0005072
part of: A-AFFY-43

1385635_at
type: EFO_0005072
part of: A-AFFY-43

(Assay) control lean rat A1
type: Assay
identifier: control lean rat A1
has participant: A-AFFY-43

(Assay) control lean rat A6
type: Assay
identifier: control lean rat A6
has participant: A-AFFY-43

(Assay) Diet Induced Obese rat C7
type: Assay
identifier: Diet Induced Obese rat C7
has participant: A-AFFY-43

Spp1
type: EnsemblDatabaseReference

Igfbp3
type: EnsemblDatabaseReference

1392172_at
type: EFO_0005072
part of: A-AFFY-43

1370281_at
type: EFO_0005072
part of: A-AFFY-43

Igfbp3
type: EnsemblDatabaseReference

1377246_at
type: EFO_0005072
part of: A-AFFY-43

(Factor value) DISEASE/lean control
type: EFO_0000001
property type: DISEASE
property value: lean control

Plet1 UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.11492830816914
p value: 0.0259299344027239
refers to: 1374594_at
refers to: Plet1
t statistic: 3.834545166957

Gpnmb UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.90635849275947
p value: 0.0111043418782167
refers to: Gpnmb
refers to: 1368187_at
t statistic: 5.78202135516643

Gpnmb
type: EnsemblDatabaseReference

(Sample) organism/Rattus norvegicus
type: Rattus norvegicus
property type: organism
property value: Rattus norvegicus

(Assay) Diet Induced Obese rat C5
type: Assay
identifier: Diet Induced Obese rat C5
has participant: A-AFFY-43

(Assay) Diet Induced Obese rat C8
type: Assay
identifier: Diet Induced Obese rat C8
has participant: A-AFFY-43

Timp1
type: EnsemblDatabaseReference

(Assay) Diet Induced Obese rat C1
type: Assay
identifier: Diet Induced Obese rat C1
has participant: A-AFFY-43

Spp1 UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.21736306040839
p value: 0.0139132421447475
refers to: 1367581_a_at
refers to: Spp1
t statistic: 5.11338728586259

Gpnmb
type: EnsemblDatabaseReference

Atp1b1 UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.21585047034558
p value: 0.0172988509005098
refers to: Atp1b1
refers to: 1367814_at
t statistic: 4.42779812202126

1390383_at
type: EFO_0005072
part of: A-AFFY-43

Analysis of 'diet induced obesity' vs 'lean control'
type: MicroarrayDifferentialExpressionAnalysis

Atp1b1
type: EnsemblDatabaseReference

Cd5l
type: EnsemblDatabaseReference

1374594_at
type: EFO_0005072
part of: A-AFFY-43

Plet1
type: EnsemblDatabaseReference

Slc22a3
type: EnsemblDatabaseReference

Plin2
type: EnsemblDatabaseReference

1367581_a_at
type: EFO_0005072
part of: A-AFFY-43

1367814_at
type: EFO_0005072
part of: A-AFFY-43

1368294_at
type: EFO_0005072
part of: A-AFFY-43

Itgax
type: EnsemblDatabaseReference

1380077_at
type: EFO_0005072
part of: A-AFFY-43

(Assay) control lean rat A4
type: Assay
identifier: control lean rat A4
has participant: A-AFFY-43

Plin2
type: EnsemblDatabaseReference

Itgax
type: EnsemblDatabaseReference

(Assay) control lean rat A8
type: Assay
identifier: control lean rat A8
has participant: A-AFFY-43

(Sample) strain/Long-Evans
type: EFO_0000001
property type: strain
property value: Long-Evans

Slc22a3 DOWN in 'diet induced obesity' vs 'lean control'
type: DecreasedDifferentialExpressionRatio
fold change: -1.18253889845402
p value: 0.0172988509005098
refers to: 1387189_at
refers to: Slc22a3
t statistic: -4.46839668225839

Ccl9
type: EnsemblDatabaseReference

Timp1 UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.02077104439056
p value: 0.0139132421447475
refers to: 1367712_at
refers to: Timp1
t statistic: 5.0228885988204

Timp1
type: EnsemblDatabaseReference

1367652_at
type: EFO_0005072
part of: A-AFFY-43

Dnase1l3
type: EnsemblDatabaseReference

Igfbp3 UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.10103644959285
p value: 0.0161435836475071
refers to: Igfbp3
refers to: 1367652_at
t statistic: 4.61953844209411

(Assay) control lean rat A3
type: Assay
identifier: control lean rat A3
has participant: A-AFFY-43

Scd1
type: EnsemblDatabaseReference

Scd1 UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 2.58278768250673
p value: 0.0111043418782167
refers to: Scd1
refers to: 1370355_at
t statistic: 5.60024096606593

Rattus norvegicus
type: OBI_0100026

Krt79
type: EnsemblDatabaseReference

(Assay) Diet Induced Obese rat C3
type: Assay
identifier: Diet Induced Obese rat C3
has participant: A-AFFY-43

(Assay) Diet Induced Obese rat C2
type: Assay
identifier: Diet Induced Obese rat C2
has participant: A-AFFY-43

1370355_at
type: EFO_0005072
part of: A-AFFY-43

Cd5l
type: EnsemblDatabaseReference

Dnase1l3 UP in 'diet induced obesity' vs 'lean control'
type: IncreasedDifferentialExpressionRatio
fold change: 1.04686001242522
p value: 0.0155623478976827
refers to: Dnase1l3
refers to: 1368294_at
t statistic: 4.67294065663189

Krt79
type: EnsemblDatabaseReference

1367712_at
type: EFO_0005072
part of: A-AFFY-43

Plet1
type: EnsemblDatabaseReference

Slc22a3
type: EnsemblDatabaseReference