F930017D23Rik DOWN in 'Klf1-/-' vs 'wild type'
Tmem107 DOWN in 'Klf1-/-' vs 'wild type'
Spata13 DOWN in 'Klf1-/-' vs 'wild type'
Fam111a DOWN in 'Klf1-/-' vs 'wild type'
Cyp3a16 DOWN in 'Klf1-/-' vs 'wild type'
Slc25a21 DOWN in 'Klf1-/-' vs 'wild type'
Gm17275 DOWN in 'Klf1-/-' vs 'wild type'
Gm26623 DOWN in 'Klf1-/-' vs 'wild type'
Zdhhc15 DOWN in 'Klf1-/-' vs 'wild type'
Arhgap21 DOWN in 'Klf1-/-' vs 'wild type'
Ppp1r15a DOWN in 'Klf1-/-' vs 'wild type'
RP23-362B7.1 DOWN in 'Klf1-/-' vs 'wild type'
Asb17os DOWN in 'Klf1-/-' vs 'wild type'
Carhsp1 DOWN in 'Klf1-/-' vs 'wild type'
Hist3h2ba DOWN in 'Klf1-/-' vs 'wild type'
Gm13881 DOWN in 'Klf1-/-' vs 'wild type'
Dnajb13 DOWN in 'Klf1-/-' vs 'wild type'
Gm16538 DOWN in 'Klf1-/-' vs 'wild type'
Ccdc109b DOWN in 'Klf1-/-' vs 'wild type'
AI182371 DOWN in 'Klf1-/-' vs 'wild type'
Sh3glb1 DOWN in 'Klf1-/-' vs 'wild type'
Gm10603 DOWN in 'Klf1-/-' vs 'wild type'
2900011O08Rik UP in 'Klf1-/-' vs 'wild type'
Smpdl3a DOWN in 'Klf1-/-' vs 'wild type'
9630028B13Rik UP in 'Klf1-/-' vs 'wild type'
(Sample) developmental stage/embryonic day 14.5
Plekhg5 DOWN in 'Klf1-/-' vs 'wild type'
Ranbp10 DOWN in 'Klf1-/-' vs 'wild type'
Hist1h1b DOWN in 'Klf1-/-' vs 'wild type'
1700063H04Rik UP in 'Klf1-/-' vs 'wild type'
1700034H15Rik UP in 'Klf1-/-' vs 'wild type'
Osbpl1a DOWN in 'Klf1-/-' vs 'wild type'
1300017J02Rik DOWN in 'Klf1-/-' vs 'wild type'
2600006K01Rik DOWN in 'Klf1-/-' vs 'wild type'
D1Ertd622e DOWN in 'Klf1-/-' vs 'wild type'
Slc26a1 DOWN in 'Klf1-/-' vs 'wild type'
Ccdc74a DOWN in 'Klf1-/-' vs 'wild type'
RP24-330E8.1 UP in 'Klf1-/-' vs 'wild type'
4930455G09Rik DOWN in 'Klf1-/-' vs 'wild type'
Ccdc103 DOWN in 'Klf1-/-' vs 'wild type'
Runx1t1 DOWN in 'Klf1-/-' vs 'wild type'
Sertad2 DOWN in 'Klf1-/-' vs 'wild type'
Gm20603 DOWN in 'Klf1-/-' vs 'wild type'
Fam210b DOWN in 'Klf1-/-' vs 'wild type'
Zdhhc14 DOWN in 'Klf1-/-' vs 'wild type'
E130308A19Rik UP in 'Klf1-/-' vs 'wild type'
Slc22a4 DOWN in 'Klf1-/-' vs 'wild type'
Tmem134 DOWN in 'Klf1-/-' vs 'wild type'
Rnaseh2b DOWN in 'Klf1-/-' vs 'wild type'
Dpy19l1 DOWN in 'Klf1-/-' vs 'wild type'
Gabarapl2 DOWN in 'Klf1-/-' vs 'wild type'
Pla2g12a DOWN in 'Klf1-/-' vs 'wild type'
Pacsin2 DOWN in 'Klf1-/-' vs 'wild type'
5830415F09Rik DOWN in 'Klf1-/-' vs 'wild type'
Serpina1c DOWN in 'Klf1-/-' vs 'wild type'
Pla2g4c DOWN in 'Klf1-/-' vs 'wild type'
Ccdc71l DOWN in 'Klf1-/-' vs 'wild type'
E130309D14Rik UP in 'Klf1-/-' vs 'wild type'
Pla2g16 DOWN in 'Klf1-/-' vs 'wild type'
RP24-217M7.2 DOWN in 'Klf1-/-' vs 'wild type'
2810417H13Rik DOWN in 'Klf1-/-' vs 'wild type'
Cdc42ep4 DOWN in 'Klf1-/-' vs 'wild type'
Tinagl1 DOWN in 'Klf1-/-' vs 'wild type'
Fam220a DOWN in 'Klf1-/-' vs 'wild type'
Slc25a38 DOWN in 'Klf1-/-' vs 'wild type'
D030025P21Rik UP in 'Klf1-/-' vs 'wild type'
Rundc3a DOWN in 'Klf1-/-' vs 'wild type'
Slc29a1 DOWN in 'Klf1-/-' vs 'wild type'
Gm12866 DOWN in 'Klf1-/-' vs 'wild type'
Tnfrsf21 DOWN in 'Klf1-/-' vs 'wild type'
Fam132b DOWN in 'Klf1-/-' vs 'wild type'
Klf1 (formerly known as Eklf) regulates the development of erythroid cells from bi-potent progenitor cells via the transcriptional activation of a diverse set of genes. Mice lacking Klf1 die in utero prior to E15 from severe anemia due to the inadequate expression of genes controlling hemoglobin production, cell membrane and cytoskeletal integrity, and the cell cycle and proliferation. We have recently described the full repertoire of Klf1 binding sites in vivo by performing Klf1 ChIP-seq in primary erythroid tissue (E14.5 fetal liver). Here we describe the Klf1-dependent erythroid transcriptome by comparing mRNA-seq from Klf1+/+ and Klf1-/- erythroid tissue. This has revealed novel target genes not previously obtainable by traditional microarray technology and provided novel insights into the function of Klf1 as a transcriptional activator such as interactions with Gata1, Scl/Tal1 and p300. We also describe a set of erythroid specific promoters not previously identified that drive high level expression of otherwise ubiquitously expressed genes in erythroid cells. Additionally, our study has identified for the first time two novel lnc-RNAs that are dynamically expressed during erythroid differentiation as well as a role for Klf1 in directing apoptotic gene expression to drive the terminal stages of erythroid maturation. Examination of mRNA expression in 3 Klf1-/- and 3 Klf1+/+ fetal livers This submission represents mRNA-Seq component of study.
Dnttip1 DOWN in 'Klf1-/-' vs 'wild type'
(Factor value) GENOTYPE/wild type genotype
Slc25a39 DOWN in 'Klf1-/-' vs 'wild type'
Rab3il1 DOWN in 'Klf1-/-' vs 'wild type'
Ankrd54 DOWN in 'Klf1-/-' vs 'wild type'
Trp53inp1 UP in 'Klf1-/-' vs 'wild type'
4930524J08Rik DOWN in 'Klf1-/-' vs 'wild type'
Slc16a1 DOWN in 'Klf1-/-' vs 'wild type'
2700046G09Rik UP in 'Klf1-/-' vs 'wild type'
Gm20033 DOWN in 'Klf1-/-' vs 'wild type'
Gm15774 DOWN in 'Klf1-/-' vs 'wild type'
D930028M14Rik DOWN in 'Klf1-/-' vs 'wild type'
Galnt10 DOWN in 'Klf1-/-' vs 'wild type'
A330094K24Rik UP in 'Klf1-/-' vs 'wild type'
Ammecr1 DOWN in 'Klf1-/-' vs 'wild type'
Slc43a1 DOWN in 'Klf1-/-' vs 'wild type'
Ranbp17 DOWN in 'Klf1-/-' vs 'wild type'
2610027K06Rik DOWN in 'Klf1-/-' vs 'wild type'
Slc35g1 DOWN in 'Klf1-/-' vs 'wild type'
AV099323 DOWN in 'Klf1-/-' vs 'wild type'
Itpripl1 DOWN in 'Klf1-/-' vs 'wild type'
C430049B03Rik UP in 'Klf1-/-' vs 'wild type'
3300005D01Rik UP in 'Klf1-/-' vs 'wild type'
Rab11fip1 UP in 'Klf1-/-' vs 'wild type'
G430095P16Rik DOWN in 'Klf1-/-' vs 'wild type'
Tcp11l2 DOWN in 'Klf1-/-' vs 'wild type'
Pip4k2c DOWN in 'Klf1-/-' vs 'wild type'
Tnfrsf25 DOWN in 'Klf1-/-' vs 'wild type'
Fam161a DOWN in 'Klf1-/-' vs 'wild type'
Mid1ip1 DOWN in 'Klf1-/-' vs 'wild type'
Slc22a21 DOWN in 'Klf1-/-' vs 'wild type'
Camsap1 DOWN in 'Klf1-/-' vs 'wild type'
5730508B09Rik DOWN in 'Klf1-/-' vs 'wild type'
Gm17080 DOWN in 'Klf1-/-' vs 'wild type'
4930430F08Rik DOWN in 'Klf1-/-' vs 'wild type'
Sertad3 DOWN in 'Klf1-/-' vs 'wild type'
Slc6a20a DOWN in 'Klf1-/-' vs 'wild type'
Ehbp1l1 DOWN in 'Klf1-/-' vs 'wild type'
4930523C07Rik UP in 'Klf1-/-' vs 'wild type'
2010001A14Rik UP in 'Klf1-/-' vs 'wild type'
3110057O12Rik UP in 'Klf1-/-' vs 'wild type'
Hist1h2ak DOWN in 'Klf1-/-' vs 'wild type'
Dnajc11 DOWN in 'Klf1-/-' vs 'wild type'
St3gal5 DOWN in 'Klf1-/-' vs 'wild type'
Slc16a10 DOWN in 'Klf1-/-' vs 'wild type'
Cacna1g DOWN in 'Klf1-/-' vs 'wild type'
4632428N05Rik DOWN in 'Klf1-/-' vs 'wild type'
Sec14l2 DOWN in 'Klf1-/-' vs 'wild type'
Ndufa12 DOWN in 'Klf1-/-' vs 'wild type'
Ldlrad4 DOWN in 'Klf1-/-' vs 'wild type'
1700001L05Rik DOWN in 'Klf1-/-' vs 'wild type'
Apol11b DOWN in 'Klf1-/-' vs 'wild type'
Phospho1 DOWN in 'Klf1-/-' vs 'wild type'
Tnfaip8l1 UP in 'Klf1-/-' vs 'wild type'
3110083C13Rik UP in 'Klf1-/-' vs 'wild type'
Hspa12b DOWN in 'Klf1-/-' vs 'wild type'
Slc25a37 DOWN in 'Klf1-/-' vs 'wild type'
Arhgef12 DOWN in 'Klf1-/-' vs 'wild type'
Gm14198 DOWN in 'Klf1-/-' vs 'wild type'