AT4G27652 DOWN in 'cys-c1 mutant' vs 'wild type'
AT4G10270 UP in 'cys-c1 mutant' vs 'wild type'
XTH26 DOWN in 'cys-c1 mutant' vs 'wild type'
PLP2 DOWN in 'cys-c1 mutant' vs 'wild type'
MDHAR UP in 'cys-c1 mutant' vs 'wild type'
HRE1 UP in 'cys-c1 mutant' vs 'wild type'
S-ACP-DES6 UP in 'cys-c1 mutant' vs 'wild type'
AT4G22610 UP in 'cys-c1 mutant' vs 'wild type'
E12A11 UP in 'cys-c1 mutant' vs 'wild type'
XTR9 DOWN in 'cys-c1 mutant' vs 'wild type'
atnudt21 DOWN in 'cys-c1 mutant' vs 'wild type'
AT1G59850 DOWN in 'cys-c1 mutant' vs 'wild type'
CBF2 DOWN in 'cys-c1 mutant' vs 'wild type'
AT2G06390 DOWN in 'cys-c1 mutant' vs 'wild type'
AT1G15010 DOWN in 'cys-c1 mutant' vs 'wild type'
PMZ DOWN in 'cys-c1 mutant' vs 'wild type'
RHS16 DOWN in 'cys-c1 mutant' vs 'wild type'
BCAT7 UP in 'cys-c1 mutant' vs 'wild type'
pEARLI 1 UP in 'cys-c1 mutant' vs 'wild type'
ACS6 DOWN in 'cys-c1 mutant' vs 'wild type'
AT4G27657 DOWN in 'cys-c1 mutant' vs 'wild type'
AT3G59710 UP in 'cys-c1 mutant' vs 'wild type'
MRH6 DOWN in 'cys-c1 mutant' vs 'wild type'
ARD UP in 'cys-c1 mutant' vs 'wild type'
AT2G23270 DOWN in 'cys-c1 mutant' vs 'wild type'
AT4G33720 UP in 'cys-c1 mutant' vs 'wild type'
PER44 DOWN in 'cys-c1 mutant' vs 'wild type'
CBF1 DOWN in 'cys-c1 mutant' vs 'wild type'
Iqd4 DOWN in 'cys-c1 mutant' vs 'wild type'
AT1G61340 DOWN in 'cys-c1 mutant' vs 'wild type'
RHS13 DOWN in 'cys-c1 mutant' vs 'wild type'
AT1G21120 DOWN in 'cys-c1 mutant' vs 'wild type'
AT3G10040 UP in 'cys-c1 mutant' vs 'wild type'
PER10 UP in 'cys-c1 mutant' vs 'wild type'
Cyanide is stoichiometrically produced as a co-product of the ethylene biosynthesis pathway, and it is detoxified by the b-cyanoalanine synthase enzyme. The molecular and phenotypical analysis of T-DNA insertional mutants of the mitochondrial b-cyanoalanine synthase CYS-C1 suggests that discrete accumulation of cyanide is not toxic for the plant and does not alter mitochondrial respiration rates, but does act as a strong inhibitor of root hair development. The cys-c1 null allele is defective in root hair formation and accumulates cyanide in root tissues. The root hair defect is phenocopied in wild type plants by the exogenous addition of cyanide to the growth medium and is reversed by the addition of hydroxocobalamin. Hydroxocobalamin not only recovers the root phenotype of the mutant, but also the formation of ROS at the initial step of the root hair tip. Transcriptional profile analysis of the cys-c1 mutant reveals that cyanide accumulation acts as a repressor signal for several genes encoding enzymes involved in cell wall rebuilding and the formation of the root hair tip, as well as genes involved in ethylene signaling and metabolism. Our results demonstrate that mitochondrial b-cyanoalanine synthase activity is essential to maintain a low level of cyanide for proper root hair development. Using Affymetrix ATH1 GeneChips, we performed a comparative transcriptomic analysis of roots of the cys-c1 and wild type plants. Total RNA was extracted from roots of 14-days-old plants grown under identical conditions on MS medium (three biological replicates for each genotype), and these samples were used to prepare complementary RNA and and analyzed using the Affymetrix-Arabidopsis ATH1GeneChip array.
CYP81F2 DOWN in 'cys-c1 mutant' vs 'wild type'
PER59 UP in 'cys-c1 mutant' vs 'wild type'
CML40 DOWN in 'cys-c1 mutant' vs 'wild type'
BCAT4 UP in 'cys-c1 mutant' vs 'wild type'
STZ DOWN in 'cys-c1 mutant' vs 'wild type'
AT4G27654 DOWN in 'cys-c1 mutant' vs 'wild type'
AT1G55450 DOWN in 'cys-c1 mutant' vs 'wild type'
AT4G29780 DOWN in 'cys-c1 mutant' vs 'wild type'
ORA47 DOWN in 'cys-c1 mutant' vs 'wild type'
ATMRP8 UP in 'cys-c1 mutant' vs 'wild type'
AT1G61080 DOWN in 'cys-c1 mutant' vs 'wild type'
AtOCT1 UP in 'cys-c1 mutant' vs 'wild type'
PCR5 DOWN in 'cys-c1 mutant' vs 'wild type'
SUS4 UP in 'cys-c1 mutant' vs 'wild type'
RHS12 DOWN in 'cys-c1 mutant' vs 'wild type'
WRKY40 DOWN in 'cys-c1 mutant' vs 'wild type'
RRTF1 DOWN in 'cys-c1 mutant' vs 'wild type'
HAK5 UP in 'cys-c1 mutant' vs 'wild type'
ATERF6 DOWN in 'cys-c1 mutant' vs 'wild type'
CCOAMT DOWN in 'cys-c1 mutant' vs 'wild type'
anac042 DOWN in 'cys-c1 mutant' vs 'wild type'
AT2G25510 DOWN in 'cys-c1 mutant' vs 'wild type'
ZAT11 DOWN in 'cys-c1 mutant' vs 'wild type'
PRP3 DOWN in 'cys-c1 mutant' vs 'wild type'
AT2G25240 DOWN in 'cys-c1 mutant' vs 'wild type'
AT1G18740 DOWN in 'cys-c1 mutant' vs 'wild type'
AT3G02840 DOWN in 'cys-c1 mutant' vs 'wild type'
AT1G51920 DOWN in 'cys-c1 mutant' vs 'wild type'
AT3G27220 UP in 'cys-c1 mutant' vs 'wild type'
IRT2 DOWN in 'cys-c1 mutant' vs 'wild type'
AGP3 DOWN in 'cys-c1 mutant' vs 'wild type'
CDEF1 UP in 'cys-c1 mutant' vs 'wild type'
GRF11 DOWN in 'cys-c1 mutant' vs 'wild type'
Analysis of 'cys-c1 mutant' vs 'wild type'
AGD11 DOWN in 'cys-c1 mutant' vs 'wild type'
ATCYSC1 DOWN in 'cys-c1 mutant' vs 'wild type'
ATTI1 UP in 'cys-c1 mutant' vs 'wild type'
RHS15 DOWN in 'cys-c1 mutant' vs 'wild type'
XTH12 DOWN in 'cys-c1 mutant' vs 'wild type'
SEN1 UP in 'cys-c1 mutant' vs 'wild type'
DIC2 DOWN in 'cys-c1 mutant' vs 'wild type'
ECS1 DOWN in 'cys-c1 mutant' vs 'wild type'
ATGSTU12 DOWN in 'cys-c1 mutant' vs 'wild type'
AT2G30670 UP in 'cys-c1 mutant' vs 'wild type'
COPT2 DOWN in 'cys-c1 mutant' vs 'wild type'
GLB1 UP in 'cys-c1 mutant' vs 'wild type'
AT3G29970 UP in 'cys-c1 mutant' vs 'wild type'
SHV3 DOWN in 'cys-c1 mutant' vs 'wild type'
RNS1 UP in 'cys-c1 mutant' vs 'wild type'
AT4G25790 DOWN in 'cys-c1 mutant' vs 'wild type'
AT2G41730 DOWN in 'cys-c1 mutant' vs 'wild type'
COW1 DOWN in 'cys-c1 mutant' vs 'wild type'
CAF1-9 DOWN in 'cys-c1 mutant' vs 'wild type'
ERF017 DOWN in 'cys-c1 mutant' vs 'wild type'
PER8 DOWN in 'cys-c1 mutant' vs 'wild type'
STR17 UP in 'cys-c1 mutant' vs 'wild type'
ATNAS2 UP in 'cys-c1 mutant' vs 'wild type'
CYP81D8 DOWN in 'cys-c1 mutant' vs 'wild type'
AT4G33070 UP in 'cys-c1 mutant' vs 'wild type'
TAT3 UP in 'cys-c1 mutant' vs 'wild type'
AT5G23840 UP in 'cys-c1 mutant' vs 'wild type'
CASP5 DOWN in 'cys-c1 mutant' vs 'wild type'
AT4G33730 DOWN in 'cys-c1 mutant' vs 'wild type'
FLA6 DOWN in 'cys-c1 mutant' vs 'wild type'
AT1G26380 DOWN in 'cys-c1 mutant' vs 'wild type'
BAP1 DOWN in 'cys-c1 mutant' vs 'wild type'
AT4G30320 DOWN in 'cys-c1 mutant' vs 'wild type'
PUB34 DOWN in 'cys-c1 mutant' vs 'wild type'
AT4G33560 UP in 'cys-c1 mutant' vs 'wild type'
AT4G27280 DOWN in 'cys-c1 mutant' vs 'wild type'
AT3G54580 DOWN in 'cys-c1 mutant' vs 'wild type'
AT1G21130 DOWN in 'cys-c1 mutant' vs 'wild type'
MYB15 DOWN in 'cys-c1 mutant' vs 'wild type'
AT1G03660 DOWN in 'cys-c1 mutant' vs 'wild type'
CBL8 DOWN in 'cys-c1 mutant' vs 'wild type'
(Factor value) GENOTYPE/wild type genotype