1633130_at
type: EFO_0005072
part of: A-AFFY-35

1628052_at
type: EFO_0005072
part of: A-AFFY-35

l(2)efl
type: EnsemblDatabaseReference

1638862_at
type: EFO_0005072
part of: A-AFFY-35

E-GEOD-12054
type: microarray 1colour mrna differential
RO_0002162: Drosophila melanogaster
description: Drosophila males double transcription of their single X chromosome to equalize X-linked gene expression with females, which carry two X chromosomes. Increased transcription requires the Male-Specific Lethal (MSL) complex. The MSL2 protein is essential component of the MSL complex. MSL2 is the only protein that is strictly limited to males. MSL2 together with MSL1 forms the core of the MSL complex. The global effect of msl2 mutation on gene expression was measured by microarray analysis. We found that expression of the X chromosome was decreased in msl21 male larvae, supporting the involvement of MSL2 in the up-regulation of X-linked genes. However, there was no change in expression of 4th chromosomal and heterochromatic genes. This finding is broadly comparable to reports of reduced X chromosome expression following msl2 RNAi knockdown in S2 cells. Hence MSL2 is required for up-regulation of male X chromosome where as it is not necessary for normal expression of 4th chromosomal genes and heterochromatic genes in Drosophila melanogaster males. Experiment Overall Design: Total RNA was prepared from groups of 50 (or more in case of smaller larvae) third instar larvae by TRIzol (Invitrogen) extraction and purified using the RNeasy kit (Qiagen). Three independent RNA preparations for each genotype served as templates for probe synthesis. Affymetrix Drosophila Genome 2.0 chips were hybridized to these probes (Santa Clara, CA). Affymetrix Gene expression data was background corrected, normalized and summarized into a one expression value per sample and probeset using the RMA (robust multi-array average) algorithm. Changes in gene expression (log2 fold changes) were determined by comparing the mean RMA expression values in the msl21 sample to the mean RMA expression values in the msl21/+ samples.
uses data from: http://identifiers.org/pubmed/19307603

to
type: EnsemblDatabaseReference

(Sample) sex/male
type: EFO_0001266
property type: sex
property value: male

Cyp6a17 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -4.45554793981408
p value: 0.0106537771622911
refers to: Cyp6a17
refers to: 1628052_at
t statistic: -9.21194873021554

Rac2
type: EnsemblDatabaseReference

lectin-24A
type: EnsemblDatabaseReference

1630740_at
type: EFO_0005072
part of: A-AFFY-35

Amyrel
type: EnsemblDatabaseReference

CG10140
type: EnsemblDatabaseReference

1631806_at
type: EFO_0005072
part of: A-AFFY-35

TepI
type: EnsemblDatabaseReference

1637367_at
type: EFO_0005072
part of: A-AFFY-35

ImpE1 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.79575389325874
p value: 0.01871229844898
refers to: 1625625_at
refers to: ImpE1
t statistic: 7.2911088586975

Cht4 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 2.17084155218079
p value: 0.0127904520937539
refers to: 1631472_at
refers to: Cht4
t statistic: 8.14812486493468

to DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.51452010423156
p value: 0.0106537771622911
refers to: 1635636_at
refers to: to
t statistic: -9.6647738023741

CG8420 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.80718561682939
p value: 0.0489156577282589
refers to: CG8420
refers to: 1625738_at
t statistic: 5.04758515429226

Drosophila melanogaster
type: OBI_0100026

CG8736 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.49734836221362
p value: 0.0382091772899182
refers to: CG8736
refers to: 1637483_at
t statistic: -5.48511816736141

LysP
type: EnsemblDatabaseReference

1632705_at
type: EFO_0005072
part of: A-AFFY-35

Cht4
type: EnsemblDatabaseReference

Cyp6d5
type: EnsemblDatabaseReference

CG11893
type: EnsemblDatabaseReference

1639401_at
type: EFO_0005072
part of: A-AFFY-35

1634239_at
type: EFO_0005072
part of: A-AFFY-35

CG40486
type: EnsemblDatabaseReference

1637734_at
type: EFO_0005072
part of: A-AFFY-35

Mur89F
type: EnsemblDatabaseReference

Mal-A4
type: EnsemblDatabaseReference

CG8420
type: EnsemblDatabaseReference

CG8736
type: EnsemblDatabaseReference

Mal-A1
type: EnsemblDatabaseReference

1631446_at
type: EFO_0005072
part of: A-AFFY-35

CG14777
type: EnsemblDatabaseReference

1623775_at
type: EFO_0005072
part of: A-AFFY-35

tobi
type: EnsemblDatabaseReference

(Sample) genotype/yw/Yy+;msl21/msl21
type: EFO_0000001
property type: genotype
property value: yw/Yy+;msl21/msl21

CG31089
type: EnsemblDatabaseReference

Cht9 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.30037615508054
p value: 0.0123404106790209
refers to: Cht9
refers to: 1631446_at
t statistic: 8.49020361380334

tobi UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 2.52775976587814
p value: 0.0140845220492684
refers to: tobi
refers to: 1623521_at
t statistic: 7.72121358521185

Mal-A6 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 3.19617625730844
p value: 0.0106685794274067
refers to: 1637567_at
refers to: Mal-A6
t statistic: 8.993610905269

CG10799
type: EnsemblDatabaseReference

1635661_at
type: EFO_0005072
part of: A-AFFY-35

Rac2 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.53528840162662
p value: 0.00327140098343885
refers to: 1624321_at
refers to: Rac2
t statistic: -13.4575945544194

1637647_at
type: EFO_0005072
part of: A-AFFY-35

1636681_at
type: EFO_0005072
part of: A-AFFY-35

1629182_at
type: EFO_0005072
part of: A-AFFY-35

ImpE1
type: EnsemblDatabaseReference

CG3191
type: EnsemblDatabaseReference

Mal-A1 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 3.06027399026001
p value: 0.0317103445122805
refers to: 1639401_at
refers to: Mal-A1
t statistic: 6.02963744473234

Mal-A4 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.74506906186074
p value: 0.0489156577282589
refers to: 1631806_at
refers to: Mal-A4
t statistic: 5.05554997099731

Cyp6a17
type: EnsemblDatabaseReference

CG40486 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.85762462159414
p value: 0.00950472656626898
refers to: 1628272_a_at
refers to: CG40486
t statistic: -10.0718754533963

mey
type: EnsemblDatabaseReference

1629343_at
type: EFO_0005072
part of: A-AFFY-35

Cyp4p2
type: EnsemblDatabaseReference

l(2)efl DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -2.08838254708681
p value: 0.0137916379042444
refers to: l(2)efl
refers to: 1635952_at
t statistic: -7.79551720742278

CG10154
type: EnsemblDatabaseReference

CG13461 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.62497688713669
p value: 0.0485074411167779
refers to: CG13461
refers to: 1635959_at
t statistic: 5.10402640651906

Mal-A8
type: EnsemblDatabaseReference

CG42640
type: EnsemblDatabaseReference

CG11893 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -5.05132735616859
p value: 0.0017186004847576
refers to: CG11893
refers to: 1635512_at
t statistic: -17.4092529389113

CG14205 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 2.83552029371112
p value: 0.0359918638888583
refers to: CG14205
refers to: 1634239_at
t statistic: 5.64671165142479

Rac2
type: EnsemblDatabaseReference

to
type: EnsemblDatabaseReference

alphaPS4
type: EnsemblDatabaseReference

1625625_at
type: EFO_0005072
part of: A-AFFY-35

CG15553
type: EnsemblDatabaseReference

lectin-24A
type: EnsemblDatabaseReference

Amyrel
type: EnsemblDatabaseReference

1624914_at
type: EFO_0005072
part of: A-AFFY-35

1637708_a_at
type: EFO_0005072
part of: A-AFFY-35

1636767_at
type: EFO_0005072
part of: A-AFFY-35

neo
type: EnsemblDatabaseReference

CG14621
type: EnsemblDatabaseReference

CG31676
type: EnsemblDatabaseReference

CG42640 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -2.01181984442358
p value: 0.0259660463401229
refers to: CG42640
refers to: 1628298_at
t statistic: -6.64482545239631

CG17672 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.32563906496128
p value: 0.0433987230912876
refers to: CG17672
refers to: 1637708_a_at
t statistic: 5.27367705554584

Cht4
type: EnsemblDatabaseReference

1630288_a_at
type: EFO_0005072
part of: A-AFFY-35

neo UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 2.38764543678314
p value: 0.0317103445122805
refers to: neo
refers to: 1638610_at
t statistic: 6.04492643447943

1630255_at
type: EFO_0005072
part of: A-AFFY-35

CG42822
type: EnsemblDatabaseReference

1623743_at
type: EFO_0005072
part of: A-AFFY-35

Mur89F
type: EnsemblDatabaseReference

1637857_at
type: EFO_0005072
part of: A-AFFY-35

CG3588
type: EnsemblDatabaseReference

CG8736
type: EnsemblDatabaseReference

Mal-A1
type: EnsemblDatabaseReference

(Sample) genotype/yw/Yy+;msl21/+
type: EFO_0000001
property type: genotype
property value: yw/Yy+;msl21/+

CG14777
type: EnsemblDatabaseReference

ninaD
type: EnsemblDatabaseReference

1628272_a_at
type: EFO_0005072
part of: A-AFFY-35

1625738_at
type: EFO_0005072
part of: A-AFFY-35

CG31089
type: EnsemblDatabaseReference

1623226_at
type: EFO_0005072
part of: A-AFFY-35

Zip3 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.28702103703924
p value: 0.0150080142279083
refers to: 1637577_at
refers to: Zip3
t statistic: 7.5976195552061

CG10799
type: EnsemblDatabaseReference

CG17672
type: EnsemblDatabaseReference

TepI DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -2.63612495123137
p value: 0.026228756448235
refers to: 1637734_at
refers to: TepI
t statistic: -6.43666126972273

1638562_a_at
type: EFO_0005072
part of: A-AFFY-35

mey UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.53681300229703
p value: 0.038973454408234
refers to: mey
refers to: 1624990_at
t statistic: 5.43915600472069

CG3191
type: EnsemblDatabaseReference

CG3588 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.01294983774395
p value: 0.0489156577282589
refers to: CG3588
refers to: 1630288_a_at
t statistic: 5.05793789284705

Mur89F UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.53681410889837
p value: 0.0123404106790209
refers to: 1629343_at
refers to: Mur89F
t statistic: 8.28531861281321

CR33318
type: EnsemblDatabaseReference

(Factor value) GENOTYPE/msl2 mutant
type: EFO_0000001
property type: GENOTYPE
property value: msl2 mutant

CG4363
type: EnsemblDatabaseReference

1631472_at
type: EFO_0005072
part of: A-AFFY-35

Cyp4p2
type: EnsemblDatabaseReference

TwdlF UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 3.93035874128231
p value: 0.0485773141478375
refers to: TwdlF
refers to: 1634284_at
t statistic: 5.09270669396381

mey
type: EnsemblDatabaseReference

Mal-A6
type: EnsemblDatabaseReference

1640566_at
type: EFO_0005072
part of: A-AFFY-35

TwdlF
type: EnsemblDatabaseReference

CG42640
type: EnsemblDatabaseReference

1637567_at
type: EFO_0005072
part of: A-AFFY-35

CG10799 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.01304189688113
p value: 0.026228756448235
refers to: CG10799
refers to: 1637367_at
t statistic: -6.35986782268338

lcs
type: EnsemblDatabaseReference

1628298_at
type: EFO_0005072
part of: A-AFFY-35

CG31148
type: EnsemblDatabaseReference

alphaPS4
type: EnsemblDatabaseReference

(Assay) larvae_msl2_mutant_3
type: Assay
has participant: A-AFFY-35

CG15553
type: EnsemblDatabaseReference

1632299_at
type: EFO_0005072
part of: A-AFFY-35

CG14621
type: EnsemblDatabaseReference

neo
type: EnsemblDatabaseReference

CR33318 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -2.25003835174877
p value: 0.0359918638888583
refers to: CR33318
refers to: 1638885_at
t statistic: -5.65926340513357

CG31676
type: EnsemblDatabaseReference

Cyp6d5 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.24328859220878
p value: 0.026228756448235
refers to: Cyp6d5
refers to: 1638562_a_at
t statistic: 6.35826499638422

CG31089 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 2.10438640138463
p value: 0.0123404106790209
refers to: CG31089
refers to: 1623775_at
t statistic: 8.28773410884611

lectin-24A DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.56597019164238
p value: 0.026228756448235
refers to: 1637857_at
refers to: lectin-24A
t statistic: -6.33980770865203

CG14205
type: EnsemblDatabaseReference

Zip3
type: EnsemblDatabaseReference

CG10140 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.0825604048444
p value: 0.0106537771622911
refers to: CG10140
refers to: 1629182_at
t statistic: -9.23315549543399

CG42822
type: EnsemblDatabaseReference

(Assay) larvae_wt_control_1
type: Assay
has participant: A-AFFY-35

1634284_at
type: EFO_0005072
part of: A-AFFY-35

CG13461
type: EnsemblDatabaseReference

CG3588
type: EnsemblDatabaseReference

1635952_at
type: EFO_0005072
part of: A-AFFY-35

ninaD
type: EnsemblDatabaseReference

1637577_at
type: EFO_0005072
part of: A-AFFY-35

1635959_at
type: EFO_0005072
part of: A-AFFY-35

Cht9
type: EnsemblDatabaseReference

CG4363 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.77487720490694
p value: 0.0323976811200344
refers to: CG4363
refers to: 1630740_at
t statistic: 5.9603433495403

l(2)efl
type: EnsemblDatabaseReference

1623521_at
type: EFO_0005072
part of: A-AFFY-35

ninaD DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.33668002583691
p value: 0.0259660463401229
refers to: 1632705_at
refers to: ninaD
t statistic: -6.61848631509664

CG14621 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.47312271754497
p value: 0.0123404106790209
refers to: CG14621
refers to: 1636681_at
t statistic: -8.53273019189893

CG14777 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.07033217715622
p value: 0.0382091772899182
refers to: CG14777
refers to: 1635661_at
t statistic: -5.52667283605536

(Assay) larvae_wt_control_3
type: Assay
has participant: A-AFFY-35

CG17672
type: EnsemblDatabaseReference

1637483_at
type: EFO_0005072
part of: A-AFFY-35

CG10140
type: EnsemblDatabaseReference

TepI
type: EnsemblDatabaseReference

Analysis of 'msl2 mutant' vs 'wild type genotype'
type: MicroarrayDifferentialExpressionAnalysis

CR33318
type: EnsemblDatabaseReference

CG4363
type: EnsemblDatabaseReference

(Assay) larvae_msl2_mutant_2
type: Assay
has participant: A-AFFY-35

Mal-A8 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 3.07133608485118
p value: 0.026228756448235
refers to: Mal-A8
refers to: 1624914_at
t statistic: 6.52291328089147

CG31148 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.7066230881946
p value: 0.0135784619488682
refers to: CG31148
refers to: 1637647_at
t statistic: 7.8656202676066

CG3191 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.05735550287394
p value: 0.0190412443366292
refers to: 1623743_at
refers to: CG3191
t statistic: -7.215200434809

Mal-A6
type: EnsemblDatabaseReference

LysP
type: EnsemblDatabaseReference

TwdlF
type: EnsemblDatabaseReference

Cyp6d5
type: EnsemblDatabaseReference

CG11893
type: EnsemblDatabaseReference

1638885_at
type: EFO_0005072
part of: A-AFFY-35

CG42822 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.48821202092457
p value: 0.0123404106790209
refers to: 1633130_at
refers to: CG42822
t statistic: 8.3975401784181

LysP UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.41971877301126
p value: 0.0135784619488682
refers to: LysP
refers to: 1636767_at
t statistic: 7.89234596846825

(Sample) organism/Drosophila melanogaster
type: Drosophila melanogaster
property type: organism
property value: Drosophila melanogaster

CG40486
type: EnsemblDatabaseReference

lcs
type: EnsemblDatabaseReference

CG8420
type: EnsemblDatabaseReference

Mal-A4
type: EnsemblDatabaseReference

CG31148
type: EnsemblDatabaseReference

Amyrel UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 2.24593939807025
p value: 0.0327595857416063
refers to: Amyrel
refers to: 1623601_at
t statistic: 5.81368985699139

lcs UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 2.27182364830835
p value: 0.026228756448235
refers to: 1641567_at
refers to: lcs
t statistic: 6.35497405639626

tobi
type: EnsemblDatabaseReference

CG10154 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.40676906495681
p value: 0.0349336759518268
refers to: 1623226_at
refers to: CG10154
t statistic: -5.71972182261576

(Assay) larvae_msl2_mutant_1
type: Assay
has participant: A-AFFY-35

(Sample) developmental stage/3rd instar larva
type: EFO_0002682
property type: developmental stage
property value: 3rd instar larva

CG15553 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.27005884032092
p value: 0.0296432283853904
refers to: CG15553
refers to: 1638862_at
t statistic: -6.16813569745265

Cyp4p2 UP in 'msl2 mutant' vs 'wild type genotype'
type: IncreasedDifferentialExpressionRatio
fold change: 1.02658818053511
p value: 0.026228756448235
refers to: Cyp4p2
refers to: 1640566_at
t statistic: 6.47107649972649

1635636_at
type: EFO_0005072
part of: A-AFFY-35

1638610_at
type: EFO_0005072
part of: A-AFFY-35

1623601_at
type: EFO_0005072
part of: A-AFFY-35

1641567_at
type: EFO_0005072
part of: A-AFFY-35

CG14205
type: EnsemblDatabaseReference

Zip3
type: EnsemblDatabaseReference

ImpE1
type: EnsemblDatabaseReference

alphaPS4 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.48918965556627
p value: 0.026228756448235
refers to: alphaPS4
refers to: 1632299_at
t statistic: -6.4320167391973

CG31676 DOWN in 'msl2 mutant' vs 'wild type genotype'
type: DecreasedDifferentialExpressionRatio
fold change: -1.98148681110127
p value: 0.00327140098343885
refers to: CG31676
refers to: 1630255_at
t statistic: -12.9042020056564

1624321_at
type: EFO_0005072
part of: A-AFFY-35

CG13461
type: EnsemblDatabaseReference

A-AFFY-35
type: EFO_0000269

(Factor value) GENOTYPE/wild type genotype
type: EFO_0000001
property type: GENOTYPE
property value: wild type genotype

Cyp6a17
type: EnsemblDatabaseReference

(Assay) larvae_wt_control_2
type: Assay
has participant: A-AFFY-35

1624990_at
type: EFO_0005072
part of: A-AFFY-35

1635512_at
type: EFO_0005072
part of: A-AFFY-35

CG10154
type: EnsemblDatabaseReference

Mal-A8
type: EnsemblDatabaseReference

Cht9
type: EnsemblDatabaseReference