AT5G62280 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
ACD6 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
LEA18 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
COR15A DOWN in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
ATCKX6 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
ECS1 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
LUP1 DOWN in 'sav3-2' vs 'wild type' in 'continuous white light'
AT4G27280 UP in 'sav1-1' vs 'wild type' in 'continuous white light'
AT1G12080 DOWN in 'sav1-1' vs 'wild type' in 'continuous white light'
CDF5 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
GA5 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
IAA29 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
RHS13 UP in 'sav1-1' vs 'wild type' in 'continuous white light'
AT5G03350 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
GH3.3 UP in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
IAA6 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
IAA2 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
SAUR68 DOWN in 'sav3-2' vs 'wild type' in 'continuous white light'
IAA5 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G32560 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G26390 UP in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
AT4G23680 DOWN in 'sav1-1' vs 'wild type' in 'continuous white light'
AT5G24210 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
LURP1 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
QQS UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G32560 DOWN in 'sav3-2' vs 'wild type' in 'continuous white light'
CML10 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
LURP1 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT3G50340 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
LEA18 DOWN in 'sav3-2' vs 'wild type' in 'continuous white light'
AT1G32560 DOWN in 'sav1-1' vs 'wild type' in 'continuous white light'
CYP85A2 UP in 'sav1-1' vs 'wild type' in 'continuous white light'
LECRK44 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
QQS UP in 'sav3-2' vs 'wild type' in 'continuous white light'
CYP71A12 UP in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
AT1G73805 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
ACD6 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT3G18200 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
PUB25 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
BHLH039 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
RGF6 DOWN in 'sav3-2' vs 'wild type' in 'continuous white light'
AT1G12080 DOWN in 'sav3-2' vs 'wild type' in 'continuous white light'
(Factor value) GROWTH_CONDITION/simulated shade for 1 hour
BHLH32 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
ATXTH17 DOWN in 'sav1-1' vs 'wild type' in 'continuous white light'
AT1G31690 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
ACS4 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G52690 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G29460 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
ECS1 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
Plants grown at high densities perceive a decrease in the red to far-red (R:FR) ratio of incoming light, resulting from absorption of red light by canopy leaves and reflection of far-red light from neighboring plants. These changes in light quality trigger a series of responses known collectively as the shade avoidance syndrome. During shade avoidance, stems elongate at the expense of leaf and storage organ expansion, there is reduced branching, and flowering is accelerated. We identified several loci in Arabidopsis, mutations in which lead to plants defective in multiple shade avoidance outputs. Here we describe SAV3, an aminotransferase, and show that SAV3 catalyzes the formation of indole-3-pyruvic acid (IPA) from L-tryptophan (L-Trp), the first step in a previously proposed, but uncharacterized, auxin biosynthetic pathway. This pathway can be rapidly deployed to biosynthesize auxin at the high levels required to initiate the multiple changes in body plan associated with shade avoidance. Experiment Overall Design: Wild type Col-0, sav3-2 and sav1-1 seedlings were used here. They were treated with or without 1hr of low R:FR light (R:FR ratio=0.7). Three independent biological replicates were used.
ICL DOWN in 'sav3-2' vs 'wild type' in 'continuous white light'
LUP1 DOWN in 'sav1-1' vs 'wild type' in 'continuous white light'
AT3G05170 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
AT2G23200 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
PSBB DOWN in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
AT5G03350 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
BAS1 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
Analysis of 'sav1-1' vs 'wild type' in 'continuous white light'
AT3G03830 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT4G29270 DOWN in 'sav1-1' vs 'wild type' in 'continuous white light'
AT2G25510 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
STZ UP in 'sav1-1' vs 'wild type' in 'continuous white light'
AT2G32560 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
IAA19 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT3G48970 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
WRKY70 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
ATPER1 DOWN in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
AT2G23200 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
GH3.3 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
RFK1 UP in 'sav1-1' vs 'wild type' in 'continuous white light'
LUP1 DOWN in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
WAK1 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT3G03840 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G52690 DOWN in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
SAUR_AC1 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT5G18060 DOWN in 'sav3-2' vs 'wild type' in 'continuous white light'
AT2G18010 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
(Factor value) GENOTYPE/wild type genotype
Analysis of 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
AT3G04210 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
CORI3 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
IAA2 DOWN in 'sav3-2' vs 'wild type' in 'continuous white light'
AT3G49960 UP in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
ADS1 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
WRKY70 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT3G04210 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G73805 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
ACS11 UP in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
RGF6 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
GH3.5 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
ACS4 DOWN in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
AT1G47400 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G47400 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
Analysis of 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
SAUR68 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
DIC2 UP in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
ARGOS DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
WAK2 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G35710 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
WAK1 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
IAA1 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT4G23680 DOWN in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
AT3G42800 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT5G62280 DOWN in 'sav3-2' vs 'wild type' in 'continuous white light'
AT5G24210 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
Analysis of 'sav3-2' vs 'wild type' in 'continuous white light'
ATERF6 UP in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
AT1G32560 DOWN in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
AT5G02760 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
LEA18 DOWN in 'sav1-1' vs 'wild type' in 'continuous white light'
CML10 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G52690 DOWN in 'sav1-1' vs 'wild type' in 'continuous white light'
AtCYS2 DOWN in 'sav1-1' vs 'wild type' in 'continuous white light'
AT2G46640 DOWN in 'sav1-1' vs 'wild type' in 'continuous white light'
STZ UP in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
AT1G29500 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G29440 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
(Factor value) GROWTH_CONDITION/continuous white light
AT1G19960 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
SIB1 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT3G28420 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G52690 DOWN in 'sav3-2' vs 'wild type' in 'continuous white light'
LUP1 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT5G41400 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT5G18060 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
YUC9 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G19960 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G78450 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT2G37030 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
ACS11 DOWN in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
AT1G35710 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
WRKY54 UP in 'sav3-2' vs 'wild type' in 'simulated shade for 1 hour'
LEA18 DOWN in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
AT4G14100 DOWN in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
AT2G25510 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
CYP81F2 UP in 'sav1-1' vs 'wild type' in 'simulated shade for 1 hour'
WRKY54 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
SIB1 UP in 'sav3-2' vs 'wild type' in 'continuous white light'
AT5G02760 DOWN in 'sav3-2' vs 'wild type' in 'continuous white light'