(Factor value) CLINICAL_INFORMATION/sham surgery
AABR06014093.1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
LOC308990 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Pik3ip1 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Rgs5 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Mme UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
(Sample) organism part/knee articular cartilage
Ifitm3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
MGC109340 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Crlf1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Fndc1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Gstp1 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Fabp5 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
F3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Crispld2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Nrtn DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Dclk1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cdh13 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Lpar6 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Adhfe1 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Marcks UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Col4a1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Lrrc17 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Kitlg UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Perp DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Herc6 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Anpep UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Abracl UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cenpf UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Hmox1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Medag UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tppp3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Panx3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Aspn UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
(Factor value) CLINICAL_INFORMATION/contralateral osteoarthritis induction
Nr1d1 DOWN in 'contralateral osteoarthritis induction' vs 'sham surgery'
Ccbl2 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Olfml1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Aspm UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Moxd1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Nfkbiz UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Pmepa1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Ccl2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Ogn UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cxcr4 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Mepe UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Mrc1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Thbs4 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Reln UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Lect1 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Kctd4 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Fabp4 UP in 'contralateral osteoarthritis induction' vs 'sham surgery'
Igf1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cidea DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Ptprc UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
LOC100911833 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Ccl7 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Arntl UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Aqp3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
OBJECTIVE: To analyze genome-wide changes in chondrocyte gene expression in a surgically induced model of early osteoarthritis (OA) in rats, to assess the similarity of this model to human OA, and to identify genes and mechanisms leading to OA pathogenesis. METHODS: OA was surgically induced in 5 rats by anterior cruciate ligament transection and partial medial meniscectomy. Sham surgery was performed in 5 additional animals, which were used as controls. Both groups underwent 4 weeks of forced mobilization, 3 times per week. RNA was extracted directly from articular chondrocytes in the OA (operated), contralateral, and sham-operated knees. Affymetrix GeneChip expression arrays were used to assess genome-wide changes in gene expression. Expression patterns of selected dysregulated genes, including Col2a1, Mmp13, Adamts5, Ctsc, Ptges, and Cxcr4, were validated by real-time polymerase chain reaction, immunofluorescence, or immunohistochemistry 2, 4, and 8 weeks after surgery. RESULTS: After normalization, comparison of OA and sham-operated samples showed 1,619 differentially expressed probe sets with changes in their levels of expression >/=1.5-fold, 722 with changes >/=2-fold, 135 with changes >/=4-fold, and 20 with changes of 8-fold. Dysregulated genes known to be involved in human OA included Mmp13, Adamts5, and Ptgs2, among others. Several dysregulated genes (e.g., Reln, Phex, and Ltbp2) had been identified in our earlier microarray study of hypertrophic chondrocyte differentiation. Other genes involved in cytokine and chemokine signaling, including Cxcr4 and Ccl2, were identified. Changes in gene expression were also observed in the contralateral knee, validating the sham operation as the appropriate control. CONCLUSION: Our results demonstrate that the animal model mimics gene expression changes seen in human OA, supporting the relevance of newly identified genes and pathways to early human OA. We propose new avenues for OA pathogenesis research and potential targets for novel OA treatments, including cathepsins and cytokine, chemokine, and growth factor signaling pathways, in addition to factors controlling the progression of chondrocyte differentiation. Experiment Overall Design: OA was surgically induced in 5 rats by anterior cruciate ligament transection and partial medial meniscectomy. Sham surgery was performed in 5 additional animals, which were used as controls. Both groups underwent 4 weeks of forced mobilization, 3 times per week. RNA was extracted directly from articular chondrocytes in the OA (operated), contralateral, and sham-operated knees, 28 days post-surgery. Affymetrix GeneChip expression arrays were used to assess genome-wide changes in gene expression.
Aqp1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Hist3h2a DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Gprc5a UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Ifit2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Ccnb2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Per2 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Dgat2 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tagln UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tnnc2 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Il1r2 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Plscr2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Sox17 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Rab32 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Robo2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Lgals1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Enpp3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Gucy1b3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cfd UP in 'contralateral osteoarthritis induction' vs 'sham surgery'
Mall UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Ramp3 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Hspb1 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Csrp2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Mylpf DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Ptges UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Lamb1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Penk DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
LOC100359539 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
P4ha3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Serping1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cthrc1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Ctsc UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tmem35 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Clec4a3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Pdlim1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
LOC171161 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tmem100 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tst DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Kdr UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Acta1 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Fcer1g UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Slc35f2 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Sdc1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
LOC290595 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Lypd6b DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Sfrp4 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Per3 DOWN in 'contralateral osteoarthritis induction' vs 'sham surgery'
Ifitm1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Aldh6a1 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Mmp13 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Col14a1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Rit2 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Etv1 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Dbp DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Postn UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Rtn1 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Arntl UP in 'contralateral osteoarthritis induction' vs 'sham surgery'
Ctss UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cd74 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Analysis of 'ipsilateral osteoarthritic induction' vs 'sham surgery'
C1s UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
C1qtnf3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Col12a1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Slfn3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cd53 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Rnd3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Rasl12 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
(Factor value) CLINICAL_INFORMATION/ipsilateral osteoarthritic induction
S1pr1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cd44 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Akr1b7 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Chi3l1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Medag UP in 'contralateral osteoarthritis induction' vs 'sham surgery'
Col1a1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Dbp DOWN in 'contralateral osteoarthritis induction' vs 'sham surgery'
AABR06022945.1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Mmp14 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tnc UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Wisp2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Klf15 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Ccna2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tmem255b DOWN in 'contralateral osteoarthritis induction' vs 'sham surgery'
Scn1a DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Fam110c DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Inhba UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Rgs4 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Myl1 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Phex UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Mmp12 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Crip1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cldn1 UP in 'contralateral osteoarthritis induction' vs 'sham surgery'
Npas2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
RT1-Db1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cubn DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tubb6 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Lsamp DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Podnl1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
(Sample) clinical information/contralateral osteoarthritis induction
Fam198b UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Fam134b UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cpxm2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Dpysl3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tnfrsf12a UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Gadd45a UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Nr1d1 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cd93 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Arhgap11a UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Chsy1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Plaur UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Angpt2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cystm1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Bcat2 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Hbegf UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Gpm6b UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Mettl7a DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Gimap5 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tro DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
RGD1306739 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
RT1-T24-4 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Hs3st1 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Pxdn UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Myoc DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
C1qtnf7 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Ptpn22 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Id4 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Dhtkd1 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Nbl1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Lbp UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Arsi UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
(Sample) clinical information/ipsilateral osteoarthritic induction
Ano1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Ednra UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Adamts4 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Igfbp3 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Prrx2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Spon1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tcn2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Mgst1 UP in 'contralateral osteoarthritis induction' vs 'sham surgery'
LOC100911874 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cybb UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Ccdc109b UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
MAMDC2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Gap43 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Hey2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Analysis of 'contralateral osteoarthritis induction' vs 'sham surgery'
Fat4 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cth DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Rnf144b UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Per3 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tfrc UP in 'contralateral osteoarthritis induction' vs 'sham surgery'
Tgfa UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
(Sample) clinical information/sham surgery
Depdc1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Cd1d1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Kif23 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
C2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
B3gnt5 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Mpv17l DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Serpina1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Pof1b DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Serpinf1 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tnfaip6 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tnn UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Prss23 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Pappa2 DOWN in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Emcn UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Tfpi2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Filip1l UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
LOC100911902 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Gbp2 UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'
Stk17b UP in 'ipsilateral osteoarthritic induction' vs 'sham surgery'